FPLC media based on cross-linked agarose beads with a mean diameter of 100 µm. The FPLC media is functionalized with NTA ligands for His-Tag.
KNAUER: FPLC Column for Tag-Affinity Chromatography
Sepapure® Affinity media for recombinantly tagged proteins is designed to be used with most aqueous buffer systems. It is long lasting when correctly handled and compatible with common CIP strategies. All Sepapure® media is stored in 20 % ethanol upon delivery. Available as prepacked 1 ml or 5 ml cartridge or as bulk material.
Agarose beads with typical loading ranges of 1–40 mg/l (Ni-NTA). The maximum operating pressure the Sepapure® columns should be used at is 3 bar, while the recommended flowrate is 1 CV/ml. Available as prepacked 1 ml or 5 ml cartridge or as bulk material.
Typically used in the first step („capture“) of an FPLC purification procedure.
In protein purification, a combination of different methods is needed for a successful separation. The purity of the wanted biomolecule is increased in three steps: Capture - Intermediate - Polishing
In the “capture step” the crude biomolecule is extracted from major side products. In the “intermediate step” further contaminations are removed, and the highly pure biomolecule is gained in the “polishing step”. For each step a different method and therefore different columns are used.
FPLC Sepapure® columns are dedicated for purification of biomolecules. Sepapure® media for affinity chromatography (AC) and Ion-Exchange chromatography (IEX) is based on Agarose and depending on the specific purification mode functionalized with ligands e.g. Protein A, Ni-NTA or quaternary ammonium (Q). These FPLC media are available packed in 1 ml and 5 ml cartridges or as bulk media.
KNAUER: FPLC Sepapure® columns
Sepapure® Desalting columns are based on Dextran with an exclusion limit of 5 kDa and available in 1 ml and 5 ml cartridges.
