KNAUER Sepapure® FPLC Columns for Ion-Exchange Chromatography

FPLC media based on cross-linked agarose beads with a mean diameter of 100 µm.  The FPLC media is functionalized with different linkers ranging from strong anion exchange ligands to weak cation exchange ligands. 

KNAUER: Sepapure FPLC Columns for Ion Exchange Chromatography

Properties:

Sepapure® Ion-Exchange media is designed to be used with most aqueous buffer systems. It is long lasting when correctly handled and is compatible with common CIP strategies. All Sepapure® media is stored in 20 % ethanol upon delivery.  

Technical data:

The agarose beads are functionalized with a quaternary ammonium for the strong anion exchanger (Q) and with diethylaminoethyl for the weak anion exchanger (DEAE). The strong cation exchanger is functionalized with sulphopropyl (SP) and the weak cation exchanger with carboxymethyl (CM). 

The maximum operating pressure the Sepapure® columns should be used at is 3 bar, while the recommended flowrate is 1 CV/ml. 

Available as prepacked 1 ml or 5 ml cartridge or as bulk material. 

Recommended application areas:

Typically used in the intermediate step of an FPLC purification procedure.

KNAUER Sepapure® 

In protein purification, a combination of different methods is needed for a successful separation. The purity of the wanted biomolecule is increased in three steps: Capture - Intermediate - Polishing

In the “capture step” the crude biomolecule is extracted from major side products. In the “intermediate step” further contaminations are removed, and the highly pure biomolecule is gained in the “polishing step”. For each step a different method and therefore different columns are used. 

FPLC Sepapure® columns are dedicated for purification of biomolecules. Sepapure® media for affinity chromatography (AC) and Ion-Exchange chromatography (IEX) is based on Agarose and depending on the specific purification mode functionalized with ligands e.g. Protein A, Ni-NTA or quaternary ammonium (Q). These FPLC media are available packed in 1 ml and 5 ml cartridges or as bulk media.

KNAUER: FPLC Sepapure® columns

Physical properties FPLC Sepapure® columns

• Resin 100 µm Agarose particles with 4 % or 6 % crosslinking
• Flowrate Recommended: 1 CV/ml
• pH Stability 3 – 9 long term
• Max. pressure 3 bar

Sepapure® Desalting columns are based on Dextran with an exclusion limit of 5 kDa and available in 1 ml and 5 ml cartridges. 

Physical properties Sepapure® Desalting columns

• Resin 20 – 50 µm Dextran particles
• Flowrate Recommended: 1 CV/ml
• Exclusion limit 5 kDa
• Max. pressure 3 bar