FPLC media based on dextran beads with a mean diameter of 20–50 µm. Size exclusion chromatography (SEC) separates molecules on the basis of size differences. KNAUER offers desalting columns based on dextran for group separations of large molecular weight biomolecules from small molecular weight compounds and salts.
KNAUER: Sepapure FPLC - Desalting or Buffer Exchange
Sepapure® Desalting media is designed to be used for removal of small molecules below the exclusion limit and for covenient rebuffering. It is long lasting when correctly handled and compatible with common CIP strategies. All Sepapure® media is stored in 20 % ethanol upon delivery. Available as prepacked 5 ml cartridge or as bulk material.
The Sepapure® dextran beads have an exclusion limit of 5 kDa. The maximum operating pressure of the Sepapure® columns is 3 bar, while the recommended flowrate is 1 CV/ml.
Typically used in the final step of an FPLC purification procedure or inbetween steps for fast buffer exchange.
In protein purification, a combination of different methods is needed for a successful separation. The purity of the wanted biomolecule is increased in three steps: Capture - Intermediate - Polishing
In the “capture step” the crude biomolecule is extracted from major side products. In the “intermediate step” further contaminations are removed, and the highly pure biomolecule is gained in the “polishing step”. For each step a different method and therefore different columns are used.
FPLC Sepapure® columns are dedicated for purification of biomolecules. Sepapure® media for affinity chromatography (AC) and Ion-Exchange chromatography (IEX) is based on Agarose and depending on the specific purification mode functionalized with ligands e.g. Protein A, Ni-NTA or quaternary ammonium (Q). These FPLC media are available packed in 1 ml and 5 ml cartridges or as bulk media.
KNAUER: FPLC Sepapure® columns
Sepapure® Desalting columns are based on Dextran with an exclusion limit of 5 kDa and available in 1 ml and 5 ml cartridges.
