Waters ACQUITY UPLC H-Class PLUS System

• Get the flexibility of multi-solvent blending - Blend up to nine solvents with integrated solvent-select valve with the Quaternary Solvent Manager 
• Deliver precise and accurate binary solvent blending - Blend up to four solvents with integrated solvent-select valve with the Binary Solvent Manager
• Get high-precision injections with direct inject sampling - The needle-in-flow path design ensures an accurate needle seal at high pressure
• Get accurate temperature control across the entire column - Configurable for 1 to 6 columns with active preheating
• Automate the formulation of mobile phases - Significantly reduce the time you spend in the prep lab with  Auto•Blend Plus Technology
• Transfer methods more easily - Easily adjust for system volume differences when transferring methods from an alternative LC system without alteration to the method table using Gradient SmartStart 
• Wide software support – ACQUITY UPLC H-Class PLUS System is supported by  Empower, MassLynx, UNIFI, and several 3rd party software packages

Recommended Use

Improving laboratory productivity by transitioning methods to UPLC technology.

Waters ACQUITY UPLC H-Class PLUS System: A highly reliable and robust LC-MS system without any compromise in speed, resolution, or sensitivity, especially in everyday settings. The ACQUITY H-Class PLUS Binary System combines an optimized flow path resistant to clogging, stable high-pressure gradient formation, and support for sub-2-µm to 5-µm particle columns to deliver the ideal choice for reproducible everyday LC-MS analyses with any tandem quad or Q-ToF.

Discover the UPLC difference

The ACQUITY UPLC H-Class PLUS System is the next evolution of ultra-performance instrumentation, providing the high resolution of a quaternary or binary liquid chromatography (LC) system.

Achieving true UPLC performance requires that system dispersion (or extra-column band spread) be matched to the peak widths associated with narrow bore columns packed with sub-2-μm particles. While other systems might be designed for high pressure, it doesn’t mean they're designed to deliver the sharp peaks of UPLC performance and the ACQUITY UPLC H-Class PLUS System.

Waters ACQUITY UPLC H-Class PLUS System: Comparison of the same method with the same column on a low-dispersion ACQUITY UPLC H-Class PLUS System versus a higher-dispersion UHPLC system.

Take your methods to the next level

Leverage the benefits of UPLC technology to improve your existing HPLC methods. By scaling your methods to UPLC, your laboratory will benefit from the combination of increased resolution, sensitivity, and speed that comes with modern small particle chemistries.

Designed to accommodate all of your LC method needs, the ACQUITY UPLC H-Class PLUS System is ideally suited to future-proof your laboratory, enabling the support of existing HPLC methods now, and provide significant productivity and cost benefit when utilized for UPLC methods.

Waters ACQUITY UPLC H-Class PLUS System: The USP analysis of diclazuril and organic impurities was scaled from HPLC to UHPLC to UPLC. The UPLC method resulted in a 4x reduction in run time and a 10x reduction in solvent consumption resulting in improved sample throughput and reduced cost per analysis.

Discover the best method for the best results

Get the versatility to discover the best set of chromatographic conditions for your sample, providing a more robust method. The flexibility of the ACQUITY UPLC H-Class PLUS Quaternary System allows you to automatically screen important method parameters, including:

• Up to six columns in independently temperature-controlled compartments
• pH and ionic strength with Auto•Blend Plus Technology
• Optional and integrated solvent selection valve expands access to six additional mobile phase lines 
• 11 unique gradient profiles

Waters ACQUITY UPLC H-Class PLUS System: (A) Auto·Blend Plus method gradient table for trastuzumab pH optimization. (B) pH optimization on trastuzumab charge variants separation. Gradient: 20 mM MES buffer, 50–155 mM NaCl in 15 min.

Deliver greater speed and precision for routine analyses

The ACQUITY UPLC H-Class PLUS Binary System combines the proven reliability and performance of the H-Class PLUS System with the precision and lowest system volume offered by our high-pressure mixing binary solvent manager, allowing you to:

• Accommodate the most challenging sample matrices
• Tackle applications with long shallow gradients
• Improve the throughput of your methods

Combine the capabilities of the ACQUITY UPLC H-Class PLUS Binary System with Waters Tandem quadruple technology for routine quantitative UPLC-MS/MS applications, so you can confidently quantify and trace components in the most complex of samples.

Waters ACQUITY UPLC H-Class PLUS System: Direct transfer without a gradient delay offers the opportunity for method improvement with the analysis time going from 5 minutes to 3 minutes representing a 40% reduction in runtime.

Specifications

• Maximum operating pressure: 15,000 psi
• pH range: 1–12.5 (For specific test conditions, contact your Waters sales representative)
• Operating flow rate range: Up to 2.2 mL/min
• Sample flow path: Stainless-steel
• Sample carryover: ≤0.002% (Caffeine) under UV conditions
• Sample compartment: 4–40 °C
• Pump options: Binary, Quaternary
• Solvent conditioning: Active Pre-heating
• Column tracking: eCord Technology tracks column information and archives column usage
• Column capacity: Up to 6 columns of 150 mm length and up to 4.6 mm I.D.
• Maximum sample capacity: Up to 20 plates (with Sample Organizer)
• Detector options: 
  • PDA Detector
  • TUV Detector
  • FLR Detector
  • RI Detector
  • ELS Detector
  • Mass Spectrometers
• External control: 
  • Empower Software
  • MassLynx Software
  • waters_connect
  • UNIFI Scientific Information System
• Physical specifications (QSM or BSM, SM-FTN, CHA):
  • Width: 34.3 cm (13.5 in.)
  • Height: 71.1 cm (28.0 in.)
  • Depth: 71.2 cm (28.0 in.)