Scientists at IOCB Prague are uncovering new details of gene transcription. They have identified a previously unknown molecular mechanism by which the transcription of genetic information from deoxyribonucleic acid (DNA) into ribonucleic acid (RNA) can be initiated. The researchers focused on a specific class of molecules known as alarmones, which are found in cells across a wide range of organisms and whose levels often increase under conditions of cellular stress. The results were published in the prestigious scientific journal Nature Chemical Biology.<img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/UOCHB_Vyzkum_z_UOCHB_odhaluje_dosud_nepoznany_zpusob_prepisu_geneticke_informace_bc2bfffa6c.jpg" alt="IOCB Prague/Tomáš Belloň: Research from IOCB Prague reveals a previously unknown mechanism of genetic transcription: Dr. Hana Cahová, head of the Chemical Biology of Nucleic Acids research group." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_UOCHB_Vyzkum_z_UOCHB_odhaluje_dosud_nepoznany_zpusob_prepisu_geneticke_informace_bc2bfffa6c.jpg 234w," sizes="100vw" width="2000" height="1335"><div>Molecules of ribonucleic acid can carry a variety of chemical modifications at one end, known as caps. In eukaryotic organisms, including human cells, the best-known cap plays an important role in RNA stability and in regulating its subsequent fate. In recent years, however, it has become clear that alternative, non-canonical RNA caps also exist, although their formation and the mechanisms by which they are attached to RNA remain only partially understood. These include alarmone caps, formed by dinucleoside polyphosphate molecules, that protect cellular RNA at moments when the cell is under threat.In their study, <a target="_blank" rel="noopener noreferrer" href="https://cahova.group.uochb.cz/en">Dr. Hana Cahová</a> and colleagues examined bacterial RNA polymerase and investigated how this enzyme can initiate transcription using dinucleoside polyphosphates (NpNs) instead of the standard RNA building blocks. For the first time, the scientists described, at the atomic level, how RNA bearing an alarmone cap can be generated directly at the initiation of gene transcription. They also observed that NpNs bind through a different type of base pairing than is typically observed.The work was significantly shaped by Valentina Serianni from Hana Cahová’s team, who demonstrated the ability of dinucleoside polyphosphates to initiate gene transcription, and by Jana Škerlová, who focused on the structural analysis of RNA polymerase. Using cryogenic electron microscopy data, she showed how dinucleoside polyphosphate molecules bind within the active site of RNA polymerase – that is, the core of the enzyme where genetic information is transcribed. Together, their findings help to elucidate the processes that accompany the transcription of genetic information from DNA to RNA.Hana Cahová adds: “We’re describing something that truly occurs in cells and that we’re now able to observe directly at the level of individual molecules. This allows us to answer fundamental questions about cellular processes, such as how cells adapt to stress. RNA plays a central role in this, as it carries the cascade of information underlying any cellular response – for example, to threatening conditions caused by nutrient deprivation or temperature shock.”<img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/UOCHB_Vyzkum_z_UOCHB_odhaluje_dosud_nepoznany_zpusob_prepisu_geneticke_informace_2_0fe88ff9e9.jpg" alt="IOCB Prague/Tomáš Belloň: Research from IOCB Prague reveals a previously unknown mechanism of genetic transcription: Dr. Tomáš Kouba, head of the Cryogenic Electron Microscopy core facility." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_UOCHB_Vyzkum_z_UOCHB_odhaluje_dosud_nepoznany_zpusob_prepisu_geneticke_informace_2_0fe88ff9e9.jpg 234w," sizes="100vw" width="2000" height="1335"><div>Cryogenic electron microscopy (cryo-EM) was central to the project. This part of the work was led by Dr. Tomáš Kouba, one of the authors of the study. “Cryogenic electron microscopy allows us to freeze biological molecules in a state very close to their natural form and then determine their three-dimensional structure. This makes it possible to look directly into the active centers of enzymes and observe their function down to the atomic level,” he explains.Last year, IOCB Prague opened a new <a target="_blank" rel="noopener noreferrer" href="https://cryoem.group.uochb.cz/en">cryo-EM center</a> that is unique within the Czech research environment. State-of-the-art cryogenic electron microscopes are housed in a specially designed building and enable the study of biological processes with exceptional structural precision.<img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/UOCHB_Vyzkum_z_UOCHB_odhaluje_dosud_nepoznany_zpusob_prepisu_geneticke_informace_3_4d881e7a58.jpg" alt="IOCB Prague: Research from IOCB Prague reveals a previously unknown mechanism of genetic transcription: This work shows how NpnNs initiate bacterial transcription. Cryo-EM reveals how nucleobases pair with the template: one in canonical and one in reverse Watson–Crick mode, providing a structural basis for their function as non-canonical RNA caps." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_UOCHB_Vyzkum_z_UOCHB_odhaluje_dosud_nepoznany_zpusob_prepisu_geneticke_informace_3_4d881e7a58.jpg 245w," sizes="100vw" width="2000" height="1077"><blockquote><h4>The Original article</h4><h4><a target="_blank" rel="noopener noreferrer" href="https://www.nature.com/articles/s41589-025-02134-5">Molecular insight into 5′ RNA capping with NpnNs by bacterial RNA polymerase</a></h4>Valentina M. Serianni, Jana Škerlová, Anna Knopp Dubánková, Anton Škríba, Hana Šváchová, Tereza Vučková, Anatolij Filimoněnko, Milan Fábry, Pavlína Řezáčová, Tomáš Kouba &amp; Hana Cahova&nbsp;Nat. Chem. Biol. 21 (2026)<a target="_blank" rel="noopener noreferrer" href="https://doi.org/10.1038/s41589-025-02134-5">https://doi.org/10.1038/s41589-025-02134-5</a><h6>licensed under <a target="_blank" rel="noopener noreferrer" href="https://creativecommons.org/licenses/by/4.0/">CC-BY 4.0</a></h6></blockquote><h4>Abstract</h4>RNA capped with dinucleoside polyphosphates has been discovered in bacteria and eukaryotes only recently. The likely mechanism of this specific capping involves direct incorporation of dinucleoside polyphosphates by RNA polymerase as noncanonical initiating nucleotides. However, how these compounds bind into the active site of RNA polymerase during transcription initiation is unknown. Here, we explored transcription initiation in vitro, using a series of DNA templates in combination with dinucleoside polyphosphates and model RNA polymerase from Thermus thermophilus. We observed that the transcription start site can vary on the basis of the compatibility of the specific template and dinucleoside polyphosphate. Cryo-electron microscopy structures of transcription initiation complexes with dinucleoside polyphosphates revealed that both nucleobase moieties can pair with the DNA template. The first encoded nucleotide pairs in a canonical Watson–Crick manner, whereas the second nucleobase pairs noncanonically in a reverse Watson–Crick manner. Our work provides a structural explanation of how dinucleoside polyphosphates initiate RNA transcription.</div></div>

Research from IOCB Prague reveals a previously unknown mechanism of genetic transcription

Food adulteration is a phenomenon with a long history that has gained increasing importance in recent years due to the globalization of food supply chains and strong economic pressures. Whether it involves diluting olive oil with cheaper oils, adding sugar to honey, or the hidden use of substitutes in protein supplements, the result is a loss of consumer trust and, in some cases, a potential risk to health. Analytical chemistry plays a key role in this area by providing methods and technologies capable of identifying and quantifying target compounds as well as unwanted impurities in a wide range of food matrices.<img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Watrex_Praha_Falsovani_potravin_a_jak_ho_odhali_analyticka_chemie_6912db5492.jpg" alt="Watrex: Food Adulteration and How It Is Revealed by Analytical Chemistry" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Watrex_Praha_Falsovani_potravin_a_jak_ho_odhali_analyticka_chemie_6912db5492.jpg 245w," sizes="100vw" width="1600" height="900"><h4>The Role of Chromatography in Forensic Food Analysis</h4>Chromatography, particularly liquid chromatography (HPLC), is one of the most effective analytical tools for separating complex mixtures of molecules. It enables:<ul><li>qualitative identification of individual components in a sample,</li><li>quantitative determination of their concentrations,</li><li>detection of impurities and unexpected components that may indicate adulteration.</li></ul>In practice, HPLC is often combined with various types of detectors (UV/VIS, DAD, refractive index detectors) or with other techniques (e.g., mass spectrometry) to increase selectivity and sensitivity.<h4>Isocratic vs. Gradient Elution</h4>In the analysis of food samples, chromatographic methods are selected according to the analytical goal:<ul><li>Isocratic HPLC uses a constant mobile phase composition throughout the analysis. It is simple, robust, and ideal for routine determinations of well-defined target analytes (e.g., sugars or vitamins).</li><li>Gradient HPLC changes the mobile phase composition during separation, enabling better resolution of complex mixtures with a wide range of polarities (e.g., peptides or phenolic compounds).</li></ul>Gradient methods are particularly useful when analytes have very different properties and high separation efficiency is required.<h4>Watrex Praha and the Contribution of Modern Systems to Food Analysis</h4><a target="_blank" rel="noopener noreferrer" href="https://watrex.com/?utm_source=labrulez&amp;utm_medium=propagace&amp;utm_campaign=labrulez_clanky_HPLCsystemy">Watrex Praha, s.r.o.</a> is one of the leading Czech manufacturers and distributors of liquid chromatography systems and instruments, with a long tradition dating back to 1990. The company has successfully transformed from a distributor into a manufacturer of its own HPLC and related technologies, focusing on modular, robust systems suitable even for demanding analytical tasks.Their <a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/products?manufacturerItems=93&amp;search=streamline">Streamline™ product line</a> includes several HPLC system concepts suitable for food analysis:<ul><li>Isocratic HPLC system – a simple yet precise configuration with a single pump, column thermostat, and UV/VIS detector; suitable for routine determination of known analytes (e.g., basic carbohydrates, food additives).</li><li>Binary gradient HPLC system – equipped with two pumps enabling mobile phase gradient formation, which enhances separation performance for complex mixtures (e.g., phenolic profiles of wine, components of aromatic extracts).</li><li>GPC/SEC system (Gel Permeation / Size Exclusion Chromatography) – a specialized solution for the determination of macromolecules and polymers, also relevant for the analysis of high-molecular-weight components in foods (e.g., polysaccharides).</li></ul><blockquote>👉<a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/labrulez-bucket-strapi-h3hsga3/Watrex_catalog_2024_ba97395f92.pdf">Product catalogue HERE</a></blockquote>Thanks to the modular nature of these systems, analysts can easily adapt the configuration to a specific analytical problem, from simple routine analyses to complex characterization of food samples.<h4>Practical Examples of Adulteration Revealed by HPLC</h4><ul><li>Detection of olive oil dilution: HPLC can separate and quantify characteristic triacylglycerols and fatty acids; unusual patterns indicate the presence of cheaper oils.</li><li>Estimation of sugar addition in honey: Isocratic HPLC with a refractive index detector can determine sugar profiles (glucose, fructose, sucrose), revealing dilution or the addition of sugar syrups.</li><li>Identification of aromatic adulterants in wine: Gradient HPLC with a PDA detector can reveal phenolic and aromatic profiles that are altered by the addition of synthetic compounds.</li></ul><h4>Conclusion</h4>Food adulteration remains a current issue that requires reliable and sensitive analytical methods. Liquid chromatography, and in particular modern HPLC systems, represent one of the most powerful tools for detecting unwanted additives, impurities, and unexpected components in complex food samples. Manufacturers such as <a target="_blank" rel="noopener noreferrer" href="https://watrex.com/?utm_source=labrulez&amp;utm_medium=propagace&amp;utm_campaign=labrulez_clanky_HPLCsystemy">Watrex Praha</a> provide flexible chromatographic platforms covering a wide range of analytical tasks from simple isocratic to advanced gradient and GPC/SEC applications thereby supporting analysts in ensuring food quality and consumer protection.<blockquote>Did you find this article interesting? Follow <a target="_blank" rel="noopener noreferrer" href="https://watrex.com/?utm_source=labrulez&amp;utm_medium=propagace&amp;utm_campaign=labrulez_clanky_HPLCsystemy">Watrex Praha</a> on their social media (<a target="_blank" rel="noopener noreferrer" href="https://www.linkedin.com/company/watrex/?viewAsMember=true">LinkedIn</a> and <a target="_blank" rel="noopener noreferrer" href="https://www.facebook.com/WatrexPrague">Facebook</a>) for more engaging topics.</blockquote>

Food Adulteration and How It Is Revealed by Analytical Chemistry

Join us for a fascinating conversation with Dr. Subhoja Chakraborty from the University of Central Florida, whose cutting-edge research targets the global challenge of malaria drug resistance. Dr. Chakraborty shares her journey from structure-based drug design during her PhD to her recent collaborative work repurposing human kinase inhibitors as rapid, selective antimalarials against drug-resistant Plasmodium falciparum strains.<h4>In this episode, you'll learn:</h4><ul><li>How structure-guided approaches reveal potential drug targets, including cysteine proteases like falcipain-2, and kinases implicated in malaria's complex life cycle.</li><li>The power of repurposing kinase inhibitors originally developed for cancer and other diseases, focusing on compound 12, which blocks hemozoin formation and protein kinase 6—key steps in parasite survival.</li><li>Lab techniques such as affinity, gel filtration, and size exclusion chromatography for recombinant protein purification—and how troubleshooting is crucial for high-yield, stable complexes.</li><li>Advances in mass spectrometry for characterizing protein interactions, validating drug targets, and mapping molecular pathways pivotal to malaria therapeutics.</li><li>Tips and lessons for new researchers facing the challenges of sample preparation, complex protocols, and working as part of a collaborative team.</li></ul>Dr. Chakraborty’s insights bridge the gap between basic science and translational drug discovery, offering hope for more effective, targeted malaria treatments in a world where resistance to frontline therapies threatens millions.<h4>Referenced Papers:</h4><ul><li>"Plasmodium falciparum protein kinase 6 and hemozoin formation are inhibited by a type II human kinase inhibitor exhibiting antimalarial activity" (Cell Chemical Biology, 2025)</li><li>"New insights of falcipain 2 structure from Plasmodium falciparum 3D7 strain" (BBRC, 2022)</li><li>"Structure-Based Optimization of Protease−Inhibitor Interactions to Enhance Specificity of Human Stefin‑A against Falcipain‑2 from the Plasmodium falciparum 3D7 Strain" (Biochemistry, 2023)</li></ul>If you’re a student, scientist, or simply passionate about advances in infectious disease research, this episode offers actionable insights, inspiring advice, and a front-row seat to innovation in combating malaria.<h3>Video Transcription</h3><h4>From Structural Biology to Drug Discovery: Chromatography and Mass Spectrometry in Malaria Research</h4><h5>Introduction</h5>In this discussion, Subhoja shares insights into her research journey, focusing on malaria drug discovery and the role of modern analytical techniques in biological and biomedical research. The conversation is aimed primarily at undergraduate students and early-career researchers interested in chemistry, biotechnology, and life sciences.<h5>Research Focus: Malaria Drug Discovery and Kinase Targets</h5>Subhoja is currently involved in malaria drug discovery research at the University of Central Florida, where she has been working since beginning her postdoctoral position two years ago. Her earlier PhD research focused on structure-based drug design in malaria, using a combination of in silico approaches, recombinant protein expression, and X-ray crystallography to determine precise three-dimensional structures of target proteins and their inhibitors.This structural perspective provided a foundation for her current work, which centers on drug discovery through compound screening and repurposing. A recent publication from her laboratory explores the use of repurposed mammalian CDK-like kinase inhibitors as potential antimalarial agents. While such kinase inhibitors are well known in cancer and cardiovascular research, their application to malaria targets represents a novel and promising approach.Kinases are attractive drug targets because of their central role in cell signaling and growth pathways. By targeting specific kinases involved in different stages of the malaria parasite life cycle—whether asexual growth, gametocytogenesis, or differentiation—it may be possible to significantly inhibit parasite growth and progression.<h5>Chromatography in Protein Expression and Purification Workflows</h5>Chromatography plays a critical role throughout Subhoja’s research. Her experience spans from basic chromatography techniques during her early training to advanced protein purification workflows using affinity chromatography, gel filtration (size exclusion chromatography), and ion exchange chromatography.Recombinant proteins are routinely purified using affinity resins such as nickel or cobalt, followed by further purification to remove contaminants. Fast Protein Liquid Chromatography (FPLC) enables compact, stepwise purification workflows suitable for producing proteins for in vitro assays.A recurring challenge in this process is the expression of high-molecular-weight proteins, which often accumulate in insoluble fractions or inclusion bodies. Careful selection of expression vectors, host systems, and induction conditions is therefore essential to maximize yield and solubility.<h5>Integrating Size Exclusion Chromatography into the Workflow</h5>Size exclusion chromatography (SEC) is typically used after affinity purification to further improve protein purity. Following elution with imidazole or glutathione, desalting is required to prevent buffer components from interfering with downstream assays. This is often achieved using desalting columns such as G-25 or PD-10.To remove lower-molecular-weight contaminants, finer SEC media—such as Superdex or Sephacryl columns—are employed. Concentration steps using molecular-weight cut-off filters are then applied to obtain the desired protein concentration for functional studies.These iterative purification and troubleshooting steps are a routine part of protein biochemistry and reflect the importance of methodical optimization and careful validation.<h5>The Role of Mass Spectrometry in Biomolecular Characterization</h5>Mass spectrometry is a key analytical tool in Subhoja’s research pipeline, particularly for target identification and validation. In both malaria and cancer research, MS is used alongside chromatography, immunoprecipitation, and pull-down assays to study protein interactions and molecular recognition events.Affinity-based mass spectrometry enables the identification of binding partners and interactors, whether studying proteins, long non-coding RNAs, or protein complexes. Techniques such as native MS, tandem affinity purification, and proximity labeling (e.g., TurboID-based workflows) provide deeper insights into molecular networks and signaling pathways.These approaches are essential for understanding how candidate drug targets function within complex biological systems.<h5>Sample Preparation Challenges in Biological Research</h5>Sample preparation remains one of the most challenging aspects of biological experimentation. Whole-cell lysates contain proteases, nucleases, and other components that can rapidly degrade target molecules. Preventing degradation requires minimizing preparation time, maintaining low temperatures, and using appropriate inhibitor cocktails.Reproducibility is also critical: biological experiments require multiple replicates, and variability is often unavoidable. Careful handling, protocol optimization, and continuous troubleshooting are essential to ensure reliable results, whether preparing samples for chromatography, mass spectrometry, sequencing, or immunoprecipitation.<h5>Advances in Chromatography and MS for Malaria Research</h5>Modern advances in chromatography and mass spectrometry significantly enhance malaria research by enabling precise target identification, quantification, and functional characterization. Drug discovery workflows begin with construct design and recombinant expression, followed by purification and interaction studies.In malaria research, stage-specific parasite biology requires careful extraction and analysis of proteins from different life cycle stages. Tandem affinity MS, immunoprecipitation, and in vivo validation studies help confirm target relevance and therapeutic potential.Recent work on repurposed kinase inhibitors has shown promising results, including inhibition of hemozoin formation—a key detoxification pathway for the malaria parasite—highlighting the potential of such compounds for further development.<h5>Malaria, Drug Resistance, and Global Impact</h5>Malaria remains a major global health burden, with over 600,000 deaths annually and widespread drug resistance complicating treatment. Artemisinin resistance, particularly associated with mutations in the K13 propeller domain, has driven the search for new targets and therapies.During her PhD, Subhoja focused on falcipain-2, a cysteine protease involved in hemoglobin degradation within the parasite. This pathway is critical for parasite survival and has emerged as a promising drug target. Her work revealed that falcipain-2 operates within a larger protein complex responsible for hemoglobin processing and amino acid release.Understanding these molecular mechanisms is essential for developing robust, stage-specific antimalarial strategies.<h5>Advice for Early-Career Researchers</h5>For researchers just starting out, Subhoja emphasizes the importance of hands-on experience. Working directly with cells, lysates, and analytical tools is essential for truly understanding experimental systems. Failure is an inherent part of research, and perseverance is key.Motivation, teamwork, careful sample handling, and thorough preparation all contribute to long-term success. Reading extensively, critically evaluating one’s own work, and appreciating incremental progress are vital aspects of a sustainable research career.Every successful experiment—no matter how small—represents meaningful progress and contributes to the broader goals of science and medicine.<h5>Closing Remarks</h5>The discussion highlights how interdisciplinary approaches combining chromatography, mass spectrometry, structural biology, and biochemistry are driving advances in malaria drug discovery. Through careful experimental design, analytical rigor, and collaborative teamwork, researchers can address complex biological challenges and contribute to global health solutions.<blockquote>This text has been automatically transcribed from a video presentation using AI technology. It may contain inaccuracies and is not guaranteed to be 100% correct.</blockquote><blockquote><h4>Concentrating on Chromatography Podcast</h4>Dive into the frontiers of chromatography, mass spectrometry, and sample preparation with host David Oliva. Each episode features candid conversations with leading researchers, industry innovators, and passionate scientists who are shaping the future of analytical chemistry. From decoding PFAS detection challenges to exploring the latest in AI-assisted liquid chromatography, this show uncovers practical workflows, sustainability breakthroughs, and the real-world impact of separation science. Whether you’re a chromatographer, lab professional, or researcher you'll discover inspiring content!You can find Concentrating on Chromatography Podcast in podcast apps:<ul><li><a target="_blank" rel="noopener noreferrer" href="https://open.spotify.com/show/1wZg67rCjoGju4yhqxI3tL">Spotify</a></li><li><a target="_blank" rel="noopener noreferrer" href="https://podcasts.apple.com/tz/podcast/concentrating-on-chromatography/id1788429185">Apple podcast</a></li><li><a target="_blank" rel="noopener noreferrer" href="https://podscan.fm/podcasts/concentrating-on-chromatography">podscan</a></li></ul>and on <a target="_blank" rel="noopener noreferrer" href="https://www.youtube.com/@ChromatographyTalk/videos">YouTube channel</a></blockquote>

Affinity, Gel Filtration, Size Exclusion Chromatography: Repurposing Kinase Inhibitors for Malaria

Article

Product

Video

Scientists

Food

Interview

Science and research

The Institute of Organic Chemistry and Biochemistry of the Czech Academy of Sciences (IOCB Prague) is a leading scientific institution in the Czech Republic, recognized internationally. Its primary mission is basic research in the fields of chemical biology and medicinal chemistry, organic and material oriented chemistry, chemistry of natural compounds, biochemistry and molecular biology, physical chemistry, theoretical chemistry, and analytical chemistry. 

Institute of Organic Chemistry and Biochemistry of the Czech Academy of Sciences

Organomation, founded in 1959, designs and manufactures high-quality nitrogen evaporators and extraction systems. Known for innovation and durability, their lab instruments are used globally for efficient sample preparation with strong customer support.

Organomation

WATREX Prague is the distributor and producer of HPLC techniques in the Czech Republic and Slovakia. Main Chromatografic and Related Techniques Supported: LC, GPC, IC, IEC, Automated SPE, LC Columns and special column packings, LC method development, Software development, Laboratory Robots.

Watrex Praha

SIELC Alltesta Mini HPLC Autosampler

The Alltesta™ Mini Autosampler is an affordable and dependable solution for automated sample handling in various analytical and liquid handling applications, including HPLC.

The Alltesta™ Mini Autosampler is an affordable and dependable solution for automated sample handling in various analytical and liquid handling applications, including HPLC. With its proprietary alpha/beta mechanical design, this compact autosampler offers direct and comprehensive control of its features, making integration quick and easy. Users can tailor the mini autosampler to their needs with customizable valve configurations, tray designs, and syringe volumes and pressures. Additionally, a built-in shaking feature enables sample mixing before injection and/or simple sample extraction within the vial.
This compact autosampler comes designed with original equipment manufacturer (OEM) customization in mind. Custom shape, color, and communication interfaces can be adapted to integrate with any system. Comprehensive software integration support is provided. Other options include metal-free sampling and reagent addition and mixing capabilities.
<ul>
<li>FRACTION COLLECTION CAPABILITY: A simple software modification can quickly convert this small autosampler into a fraction collector.
</li>
<li>REACTOR PROBE SAMPLER CAPABILITY: A simple software modification can quickly convert this small autosampler into a reactor probe sampler.
</li>
</ul>
This miniature autosampler can also be used as a component of our <a href="https://lcms.labrulez.com/products/1242">Alltesta™ Gradient Automated Analyzer</a> and <a href="https://lcms.labrulez.com/products/1243">Alltesta™ Cromite Analyzer</a>.
<blockquote>
<h4>The price of the Alltesta™ Mini Autosampler varies depending on the specific configuration requested.</h4>
</blockquote>
<h4>Specifications</h4>
<ul>
<li>Dimensions (WHL): 160 x 145 x 195 mm / 6.3 x 5.71 x 7.68 in</li>
<li>Weight: 3 kg / 6.6 lb</li>
<li>Power: 24 V</li>
<li>Capacity: 48 vials or 96-well plate</li>
<li>Pressure: 5000 psi</li>
<li>Needle Wash: Inside and outside</li>
<li>Syringe Capacity: 120µL high-pressure / 4000µL low-pressure stainless / 4000µL low-pressure ceramic</li>
<li>Valve options: 6×2, 7×6 and 10×9 (more details here).</li>
<li>Volume Accuracy: 0.1µL</li>
<li>Communication: RS232 Virtual COM port via USB</li>
</ul>
<img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/SIELC_Alltesta_Mini_Autosampler_A_very_simple_schematic_operation_of_the_Alltesta_Autosampler_b79b0ad187.jpg" alt="SIELC: Alltesta™ Mini Autosampler - A very simple schematic operation of the Alltesta™ Autosampler."/>
<h4>Software</h4>
The Alltesta™ Autosampler can be controlled:
<ul>
<li>directly via serial commands (Alltesta™ protocol available upon request, please contact us at support@sielc.com),</li>
<li>directly via our demo software, allowing to learn the whole functionality of the unit (<a href="https://sielc.com/wp-content/uploads/2022/12/Alltesta_Autosampler_Control_Windows_10.zip">Windows 10, 32/64bit</a>, 5Mb; <a href="https://sielc.com/wp-content/uploads/2022/12/Alltesta_Autosampler_Control_Windows_7.zip">Windows 7, 32/64bit</a>, 126Mb).</li>
<li>remotely through our cloud software (as a part of Alltesta™ Analyzer or Alltesta™ Mini Analyzer),</li>
</ul>
Serial communication drivers are available <a href="https://ftdichip.com/drivers/vcp-drivers/">here</a>.

KNAUER LH 8.1 - Modular analytical XYZ Autosampler

KNAUER Liquid Handler offers finally an alternative in the world of robotic autosamplers. In-house design and development for a new level of simplicity and speed.

KNAUER Liquid Handler offers finally an alternative in the world of robotic autosamplers. In-house design and development for a new level of simplicity and speed, namely by:<h4>Performance</h4><ul><li>Highest repeatability of peak area in full loop or sandwich injection mode</li><li>Zero sample loss and high precision via Sandwich injection mode&nbsp;</li><li>Lowest carry over with active Fast wash station that cleans the injection syringe and needle on the in- and outside by delivering an adjustable cleaning solvent flow</li><li>User-friendly control via pre programmed injection parameters in Chromatography Software packages</li></ul><h4>Versatility</h4><ul><li>Modular design and wide selection of accessories</li><li>Flexible injection volumes by a variety of exchangeable injection syringes&nbsp;</li><li>Customizable lengths of the rail from standard &nbsp;557 or 887 mm up to 1200mm to increase the amount of modules</li></ul><h4>High throughput</h4><ul><li>Up to 390 x 1.5 mL vials or 6 well plates per Robotic Cooler</li><li>Maximum 1560 x 1.5 ml vials or 24 well plates per Liquid Handler</li><li>Short cycle times via fast and precise movements and the software option "overlapped injections" that allows for preparing the next injection while the previous is still running</li></ul><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Knauer_Liquid_Handler_LH_8_1_AZURA_system1_7ad2b43571.jpg" alt="Knauer: Liquid Handler LH 8.1 with AZURA system" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Knauer_Liquid_Handler_LH_8_1_AZURA_system1_7ad2b43571.jpg 244w," sizes="100vw" width="1390" height="889"><h3>Great variety of modules available</h3>KNAUER offers a range of active and passive modules for easy customisation of your LH 8.1.<ul><li>Want to have more samples and need a cooling option? Use the Robotic Cooler!</li><li>Want a lower carry over? Install the Fast Wash Station with two different solvents for in- and outside syringe and needle cleaning!</li><li>Want to change the solvent in your syringe? Use a passive solvent option to draw liquid during the injection process!</li></ul>There are a variety of active and passive options available. Just mount the module on the sampler, configure it in your software and you are ready to go.<h4>Injection Valve</h4>The injection valve consists of a valve drive and a 6 Port 2-Position valve with a special injection port.<img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Knauer_Liquid_Handler_LH_8_1_standalone1_5f21514cca.jpg" alt="Knauer: Liquid Handler LH 8.1 - standalone" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Knauer_Liquid_Handler_LH_8_1_standalone1_5f21514cca.jpg 179w," sizes="100vw" width="1293" height="1127"><h4>Tray Holder</h4>The manual tray holder can store up to 130 standard HPLC vials and is recommended whenever no sample cooling is needed.<h4>Robotic Cooler</h4>The Robotic Cooler can store your samples at 4 - 40°C. You can insert up to 3 Sample Racks for 130 vials each, what means you can store up to 390 sample vials per Robotic Cooler.<img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Knauer_Liquid_Handler_LH_8_1_cooled_tray_2_c6fd0672e9.jpg" alt="Knauer: Liquid Handler LH 8.1 - cooled tray" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Knauer_Liquid_Handler_LH_8_1_cooled_tray_2_c6fd0672e9.jpg 239w," sizes="100vw" width="1595" height="1043"><h4>Fast Wash Station</h4>With the fast wash station, the injection syringe is cleaned on the in- and outside. It is always recommended, because it significantly reduces carry-over and offers a very user-friendly handling.&nbsp;<h4>Manual Wash Station</h4>Manual wash stations are an alternative to the fast wash station when carry-over is not the major concern. A manual wash station can be integrated in the manual tray holder with 5 x 10 ml vials or used as an additional module with 6 x 10 ml vials or 2 x 60 ml vials.<h4>Sample Trays</h4>Different sample trays can be used either in the manual rack holder or in the Robotic Cooler. Depending on your needs, they are made for analytical standard 1.5 ml vials or well plates.<h4>Syringes</h4>The most common syringes are implemented in the chromatography software packages. All optimized parameters are preconfigured to get quickly started.<h3>Special features</h3><h4>Injection modes</h4><h5>Sandwich loop: Zero sample loss with excellent reproducibility</h5>Tired of choosing between sample loss and high performance? The new injection mode offers both.<ul><li>KNAUER Liquid Handler LH 8.1 has a special injection mode. Next to standard injections like full loop and partial loop it has the opportunity to use the sandwich injection mode.</li><li>The sample is prepared inside a gastight syringe and then transferred into the HPLC System. In sandwich mode the sample is trapped between small gaps of air. This injection method only takes up the sample volume it needs and the air gaps prevent any peak broadening. Furthermore the pressure increase during the injection process will reduce the sample plug even more leading ot much narrower peak shapes and higher injection precision. &nbsp;</li><li>Ths injection method combines the zero sample loss of µl pickup and injection precision of partial loop injection.</li><li>Only the amount of sample that is injected is aspired by the syringe</li><li>Minimized sample widenening during injection process</li><li>Very high precision</li><li>No loss of sample</li></ul><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Knauer_Liquid_Handler_LH_8_1_Sandwich_Injection_Mode_Big_fb713e65a0.jpg" alt="Knauer - Liquid Handler LH 8.1 - Sandwich Injection Mode" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Knauer_Liquid_Handler_LH_8_1_Sandwich_Injection_Mode_Big_fb713e65a0.jpg 179w," sizes="100vw" width="2832" height="2475"><h5>Full loop</h5><ul><li>Sample loop is completely filled with the sample</li><li>Maximum reproducibility is achieved</li><li>Injection volume equals the loop volume</li><li>The sample loop will be overfilled to ensure a complete and reproducible filling</li></ul><h5>Partial loop</h5><ul><li>Sample loop is partially filled with programmed amount of sample&nbsp;</li><li>Injection mode is recommended for preparative tasks</li><li>Maximum injection volume equals 50 % of the loop volume</li><li>No sample loss</li></ul><h3>Large number of samples for high-throughput applications</h3>The Robotic Cooler offers space for up to 390 x 1.5 ml standard sample vials while maintaining a stable temperature.&nbsp;<ul><li>The Robotic Cooler compartment offers 6 places for sample racks. Two per drawer with a variety of options. One place can be used by a 60 x 1.5 ml vial rack, or 384 well plates or 96 well plates. Furthermore a double rack can be used to offer 130 x 1.5 mL vial positions, taking the total sample capacity to 390 x 1.5 ml vials.</li><li>Depending on the rail length a multitude of Robotic Coolers can be installed and used seperatly. Up to 4 Robotic Coolers on the longest rail.</li><li>The Robotic Cooler is software controlled, which enables a higher response speed and only open the doors so the injection syringe can reach the sample, to protect other samples from temperature fluctuations.&nbsp;</li></ul><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Knauer_Liquid_Handler_LH_8_1_cooled_tray_1_d2a6edf265.jpg" alt="Knauer: Liquid Handler LH 8.1 - high throughtput" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Knauer_Liquid_Handler_LH_8_1_cooled_tray_1_d2a6edf265.jpg 245w," sizes="100vw" width="1722" height="1040"><h3>Speed up your injection</h3>The LH 8.1 is optimized to save time during injection and movement.<ul><li>To safe time during the movement, the opening of the Robotic Cooler is software controlled and happens automatically.</li><li>To further speed up the injection, the sampler can apply the "overlapped injections" function, where the sample is already prepared inside the syringe.</li><li>Then the sample can be injected directly after the end of the previous run.&nbsp;</li></ul><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Knauer_Liquid_Handler_LH_8_1_Method_overlapped_2_e94a888086.jpg" alt="Knauer: Liquid Handler LH 8.1 - Method_overlapped" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Knauer_Liquid_Handler_LH_8_1_Method_overlapped_2_e94a888086.jpg 245w," sizes="100vw" width="1121" height="310"><h3>Easy control via HPLC software&nbsp;</h3><ul><li>The LH 8.1 can be used just as comfortable as any HPLC Autosampler.</li><li>All parameters for recommended syringes, like injection speed, delay and sandwich build-up are already implemented and extensively tested in our Chromatography Software packages, so that you are ready to go!</li></ul><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Knauer_Liquid_Handler_LH_8_1_Method_overlapped_af91cd2ba5.jpg" alt="Knauer: Liquid Handler LH 8.1 - SW control" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Knauer_Liquid_Handler_LH_8_1_Method_overlapped_af91cd2ba5.jpg 188w," sizes="100vw" width="928" height="771">

ECOM AS96 HPLC Autosampler

A reliable and precise autosampler for a wide range of applications in HPLC and LC-MS with/without cooling and heating, in a compact design. 

A reliable and precise autosampler for a wide range of applications in HPLC and LC-MS with/without cooling and heating, in a compact design.AS96 Autosampler is a generic autosampler using state-of-the-art injection technology with fast injection and wash cycles. Efficient, multi-solvent needle wash virtually eliminates carry-over. AS96 enables excellent reagent addition and mix capabilities for derivatization, dilution, and internal standard addition. Our Pressure Assisted Sample Aspiration (PASA™) concept avoids sample-syringe contact and air bubbles and has proven its robustness in more than 30,000 autosamplers.AS96 Autosampler handles well plates and sample vials, either open or sealed. Its compact, stackable design and unrivaled performance make the AS96 the best fit for any analytical system. The autosampler can also be equipped with a sample cooling module. The Peltier module with forced air cooling provides true sample cooling down to 4 °C in the sample compartment.<h4>Features</h4><ul><li>Metal-free sampling with silica-coated steel needle and PEEK valve (option)</li><li>Reagent addition and mix capabilities for derivatization, dilution, internal standard addition</li><li>Optional 6-port solvent selection valve (SVV) for extended selection of reagents for needle wash and reagent addition (option)</li><li>Integrated Stream Switching (ISS): optional extra high-pressure 6-port switching valve for column switching or other stream switching applications up to 18,000 Psi</li><li>Quick-fit injection valve for fast service</li></ul><h4>Parameters</h4><ul><li>PN: ASA96000</li><li>Injection modes: Full-loop, Partial loop-fill , µL-pick-up</li><li>Injection volume: Programmable from 0 µl – 9999 µL 1 µL increments</li><li>Injection precision: Full-loop injection ‹ 0.3% RSD; Partial loop-fill ‹ 0.5% RSD; µL -pick-up ‹ 1.0% RSD</li><li>Sample viscosity: 0.1 – 5 cP</li><li>Injections per vial/well: Max 9</li><li>Syringe volume: 500 µL standard; 250, 1000 and 2500 µL optional</li><li>Needle wash - Inside and outside needle wash with drying. Wash can be programmed between injections and between vials/wells: 1 solvent, 5 additional wash solvents</li><li>Injection cycle time: 60 seconds</li><li>Valve switching time: 60 msec</li><li>Wetted parts: SS316, PTFE, TEFZEL, VESPEL, glass, For Bio-kit option: PEEK and coated-steel (needle) instead of SS316</li><li>Loop volumes: Programmable from 0 µl – 9999 µL 1 µL increments&nbsp;</li><li>Carry-over: ‹ 0.05% with standard wash, Typically ‹ 0.01% with extra wash</li><li>Sample capacity: Microtiter plates: 2; Vials: 2x 48 (1.5 mL) or 2x 12 (10 mL) - optional</li><li>Maximum vial/MTP height: 47 mm</li><li>Sample cooling - Factory installed option (Peltier technology): Minimum: 4°C ± 2°C Maximum: ambient temp -3°C</li></ul>

Watrex Streamline™ Manual Injection Valve Kit

Steinless steel manual injector with integrated inlet port for Hamilton needle (0.7 mm OD), with “No-Flow-Interruption (NFI)” technology to prevent pressure pulse during valve rotation. 

Steinless steel manual injector with integrated inlet port for Hamilton needle (0.7 mm OD), with “No-Flow-Interruption (NFI)” technology to prevent pressure pulse during valve rotation.&nbsp;<blockquote>Price: 992<bdi>&nbsp;€ ex VAT</bdi></blockquote>It comes with the bracket, 20 ul loop (other volumes on request) and Hamilton type syringe.<img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Watrex_Streamline_Manual_Injection_Valve_Kit_965e688a66.jpg" alt="Watrex: Streamline™ Manual Injection Valve Kit" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Watrex_Streamline_Manual_Injection_Valve_Kit_965e688a66.jpg 208w," sizes="100vw" width="1024" height="768">You can also visit this product on the <a target="_blank" rel="noopener noreferrer" href="https://watrex.com/obchod/chromatography/sampling-devices/manual-injectors/streamline-manual-injection-valve-kit/?utm_source=labrulez&amp;utm_medium=propagace&amp;utm_campaign=labrulez_produkty">Watrex website</a>.

Watrex Streamline™ Manual Injection Valve Kit, PEEK

PEEK manual injector with integrated inlet port for Hamilton needle (0.7 mm OD), with “No-Flow-Interruption (NFI)” technology to prevent pressure pulse during valve rotation.

PEEK manual injector with integrated inlet port for Hamilton needle (0.7 mm OD), with “No-Flow-Interruption (NFI)” technology to prevent pressure pulse during valve rotation.<blockquote>Price: 1 100<bdi>&nbsp;€ ex VAT</bdi></blockquote>It comes with the bracket, 20 ul loop (other volumes on request) and Hamilton type syringe.You can also visit this product on the <a target="_blank" rel="noopener noreferrer" href="https://watrex.com/obchod/chromatography/sampling-devices/manual-injectors/streamline-manual-injection-valve-kit-2/?utm_source=labrulez&amp;utm_medium=propagace&amp;utm_campaign=labrulez_produkty">Watrex website</a>.<figure class="image"><img style="aspect-ratio:800/600;" src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Watrex_Streamline_Manual_Injection_Valve_Kit_PEEK_0b4aaf2ff2.jpg" alt="Watrex: Streamline™ Manual Injection Valve Kit, PEEK" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Watrex_Streamline_Manual_Injection_Valve_Kit_PEEK_0b4aaf2ff2.jpg 208w," sizes="100vw" width="800" height="600"></figure>

Watrex Streamline™ MIM Manual Injector Module

Streamline instrument module for manual sampling with both full and partially filled loop method. The MIM includes a six-port two position valve with a 20 µl loop and syringe port, housed in a standard Streamline cabinet.

Streamline instrument module for manual sampling with both full and partially filled loop method. The MIM includes a six-port two position valve with a 20 µl loop and syringe port, housed in a standard Streamline cabinet.<blockquote>Price: 1 510<bdi>&nbsp;€ ex VAT</bdi></blockquote>The valve is equipped with a position sensor to automatically synchronize injection with the start of data collection and with the feature. The valve architecture allows uninterrupted flow as the valve switches from LOAD to INJECT. The mobile phase continuously flows through both the rotor seal groove and the special passage until rotation stops.<img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Watrex_Streamline_MIM_Manual_Injector_Module_df092ebbaf.jpg" alt="Watrex: Streamline™ MIM Manual Injector Module" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Watrex_Streamline_MIM_Manual_Injector_Module_df092ebbaf.jpg 208w," sizes="100vw" width="1024" height="768">You can also visit this product on the <a target="_blank" rel="noopener noreferrer" href="https://watrex.com/obchod/chromatography/sampling-devices/manual-injectors/streamline-mim-manual-injector-module/?utm_source=labrulez&amp;utm_medium=propagace&amp;utm_campaign=labrulez_produkty">Watrex website</a>.

Watrex Streamline™ MIM Manual Injector Module, PEEK

Streamline instrument module for manual sampling with both full and partially filled loop method. The MIM includes a PEEK six-port two position valve with a 20 µl loop and syringe port, housed in a standard Streamline cabinet.

Streamline instrument module for manual sampling with both full and partially filled loop method. The MIM includes a PEEK six-port two position valve with a 20 µl loop and syringe port, housed in a standard Streamline cabinet.<blockquote>Price: 1 510<bdi>&nbsp;€ ex VAT</bdi></blockquote>The valve is equipped with a position sensor to automatically synchronize injection with the start of data collection and with the feature. The valve architecture allows uninterrupted flow as the valve switches from LOAD to INJECT. The mobile phase continuously flows through both the rotor seal groove and the special passage until rotation stops.<img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Watrex_Streamline_MIM_Manual_Injector_Module_df092ebbaf.jpg" alt="Watrex: Streamline™ MIM Manual Injector Module. PEEK" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Watrex_Streamline_MIM_Manual_Injector_Module_df092ebbaf.jpg 208w," sizes="100vw" width="1024" height="768">You can also visit this product on the <a target="_blank" rel="noopener noreferrer" href="https://watrex.com/obchod/chromatography/sampling-devices/manual-injectors/streamline-mim-manual-injector-module-peek/?utm_source=labrulez&amp;utm_medium=propagace&amp;utm_campaign=labrulez_produkty">Watrex website</a>.

Watrex Streamline™ HPLC AS1 Autosampler 

The AS1 autosampler with a sample capacity of 60 standard (12 x 32 mm) vials or 96 with a microtiter plate, is the smallest of the two autosamplers of the Streamline HPLC range.

The AS1 autosampler with a sample capacity of 60 standard (12 x 32 mm) vials or 96 with a microtiter plate, is the smallest of the two autosamplers of the Streamline HPLC range.<blockquote>Price: 7 100<bdi>&nbsp;€ ex VAT</bdi></blockquote>The small footprint of only 310 x 210 x 478 mm (HxWxD) in combination with excellent specifications and almost maintenance free mechanics makes this autosampler an ideal partner for automating not only Streamline HPLC systems but virtually any HPLC system that needs to be upgraded with an autosampler.&nbsp;A speciality of the AS1 autosampler is the double needle injection technique by which the outer needle or “air needle” pierces the vial septum before the sample needle enters the vial. This is a unique approach avoiding blocked injection needles and/or blocked injection valves. This allows the use of any type of vial septums and by which it adds to improved reliability of the chromatography system as a whole.You can also visit this product on the <a target="_blank" rel="noopener noreferrer" href="https://watrex.com/obchod/chromatography/sampling-devices/autosamplers/streamline-as1-autosampler/?utm_source=labrulez&amp;utm_medium=propagace&amp;utm_campaign=labrulez_produkty">Watrex website</a>.

Watrex Streamline™ HPLC AS2 Autosampler 

The AS2 autosampler with a sample capacity of 120 standard (12 x 32 mm) vials or 192 samples with microtiter plates. The excellent specifications and almost maintenance free mechanics makes this autosampler an ideal partner.

The AS2 autosampler with a sample capacity of 120 standard (12 x 32 mm) vials or 192 samples with microtiter plates. The excellent specifications and almost maintenance free mechanics makes this autosampler an ideal partner for automating not only Streamline HPLC systems but virtually any HPLC system that needs to be upgraded with an autosampler.<blockquote>Price: 7 097<bdi>&nbsp;€ ex VAT</bdi></blockquote>A speciality of the Streamline autosampler is the double needle injection technique by which the outer needle or “air needle” pierces the vial septum before the sample needle enters the vial. This is a unique approach avoiding blocked injection needles and/or blocked injection valves. This allows the use of any type of vial septums and by which it adds to improved reliability of the chromatography system as a whole.You can also visit this product on the <a target="_blank" rel="noopener noreferrer" href="https://watrex.com/obchod/chromatography/sampling-devices/autosamplers/streamline-as2-autosampler/?utm_source=labrulez&amp;utm_medium=propagace&amp;utm_campaign=labrulez_produkty">Watrex website</a>.

ECOM L3320 HPLC Autosampler

An automatic autosampler with excellent accuracy and linearity of sample injection. Innovated needle design reduces sample contamination and µl pick-up mode achieves zero sample loss.

Autosampler L3320 is an automatic autosampler with excellent accuracy and linearity of sample injection. Innovated needle design significantly reduces sample residue contamination. µl pick-up mode achieves zero sample loss.The autosampler is designed as a&nbsp;standalone unit.The user-friendly unit features computer control and shows information about the actual status on the unit display. Automated self-checking simplifies the usage of the autosampler, there is no need for calibration or needle position setting.Rheodyne valve secures the reliable operation of the device.The unit is supported by the <a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/products/999">Clarity&nbsp;software</a>.<h4>Parameters</h4><ul><li>Communication: RS232,USB</li><li>Syringe volume: Standard 250μl; 100μl optional</li><li>Reproducibility: Full loop injection: RSD≤0.3%, Partial loopfill injection: RSD≤0.5%, μl pickup injection: RSD≤1%</li><li>Control:&nbsp;PC (Clarity SW)</li><li>Loop volumes: Standard 100μl; 10μl, 20μl and 50μl optional</li><li>Injection Volume: Full loop injection = loop volume, Partial loop injection = ½ of loop volume, μl pickup injection = (loop volume – 3 x needle volume)/2</li><li>Sample Capacity: 2 standard 48-vial tray (1.5ml vials)</li><li>Pressure limit: 6000 PSI</li><li>Cross Contamination: &lt; 0.02% after programmed needle wash</li><li>Weight: 17kg</li><li>Dimensions W x H x D: 453 x 359 x 244 mm (17.8 x 14.1 x 9.6”)</li><li>Linearity: 0.9999</li><li>Power supply: 100-240VAC, 50-60Hz, 110W&nbsp;</li></ul>

Products from category HPLC Autosamplers - page 1

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