Scientists at IOCB Prague are uncovering new details of gene transcription. They have identified a previously unknown molecular mechanism by which the transcription of genetic information from deoxyribonucleic acid (DNA) into ribonucleic acid (RNA) can be initiated. The researchers focused on a specific class of molecules known as alarmones, which are found in cells across a wide range of organisms and whose levels often increase under conditions of cellular stress. The results were published in the prestigious scientific journal Nature Chemical Biology.<img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/UOCHB_Vyzkum_z_UOCHB_odhaluje_dosud_nepoznany_zpusob_prepisu_geneticke_informace_bc2bfffa6c.jpg" alt="IOCB Prague/Tomáš Belloň: Research from IOCB Prague reveals a previously unknown mechanism of genetic transcription: Dr. Hana Cahová, head of the Chemical Biology of Nucleic Acids research group." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_UOCHB_Vyzkum_z_UOCHB_odhaluje_dosud_nepoznany_zpusob_prepisu_geneticke_informace_bc2bfffa6c.jpg 234w," sizes="100vw" width="2000" height="1335"><div>Molecules of ribonucleic acid can carry a variety of chemical modifications at one end, known as caps. In eukaryotic organisms, including human cells, the best-known cap plays an important role in RNA stability and in regulating its subsequent fate. In recent years, however, it has become clear that alternative, non-canonical RNA caps also exist, although their formation and the mechanisms by which they are attached to RNA remain only partially understood. These include alarmone caps, formed by dinucleoside polyphosphate molecules, that protect cellular RNA at moments when the cell is under threat.In their study, <a target="_blank" rel="noopener noreferrer" href="https://cahova.group.uochb.cz/en">Dr. Hana Cahová</a> and colleagues examined bacterial RNA polymerase and investigated how this enzyme can initiate transcription using dinucleoside polyphosphates (NpNs) instead of the standard RNA building blocks. For the first time, the scientists described, at the atomic level, how RNA bearing an alarmone cap can be generated directly at the initiation of gene transcription. They also observed that NpNs bind through a different type of base pairing than is typically observed.The work was significantly shaped by Valentina Serianni from Hana Cahová’s team, who demonstrated the ability of dinucleoside polyphosphates to initiate gene transcription, and by Jana Škerlová, who focused on the structural analysis of RNA polymerase. Using cryogenic electron microscopy data, she showed how dinucleoside polyphosphate molecules bind within the active site of RNA polymerase – that is, the core of the enzyme where genetic information is transcribed. Together, their findings help to elucidate the processes that accompany the transcription of genetic information from DNA to RNA.Hana Cahová adds: “We’re describing something that truly occurs in cells and that we’re now able to observe directly at the level of individual molecules. This allows us to answer fundamental questions about cellular processes, such as how cells adapt to stress. RNA plays a central role in this, as it carries the cascade of information underlying any cellular response – for example, to threatening conditions caused by nutrient deprivation or temperature shock.”<img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/UOCHB_Vyzkum_z_UOCHB_odhaluje_dosud_nepoznany_zpusob_prepisu_geneticke_informace_2_0fe88ff9e9.jpg" alt="IOCB Prague/Tomáš Belloň: Research from IOCB Prague reveals a previously unknown mechanism of genetic transcription: Dr. Tomáš Kouba, head of the Cryogenic Electron Microscopy core facility." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_UOCHB_Vyzkum_z_UOCHB_odhaluje_dosud_nepoznany_zpusob_prepisu_geneticke_informace_2_0fe88ff9e9.jpg 234w," sizes="100vw" width="2000" height="1335"><div>Cryogenic electron microscopy (cryo-EM) was central to the project. This part of the work was led by Dr. Tomáš Kouba, one of the authors of the study. “Cryogenic electron microscopy allows us to freeze biological molecules in a state very close to their natural form and then determine their three-dimensional structure. This makes it possible to look directly into the active centers of enzymes and observe their function down to the atomic level,” he explains.Last year, IOCB Prague opened a new <a target="_blank" rel="noopener noreferrer" href="https://cryoem.group.uochb.cz/en">cryo-EM center</a> that is unique within the Czech research environment. State-of-the-art cryogenic electron microscopes are housed in a specially designed building and enable the study of biological processes with exceptional structural precision.<img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/UOCHB_Vyzkum_z_UOCHB_odhaluje_dosud_nepoznany_zpusob_prepisu_geneticke_informace_3_4d881e7a58.jpg" alt="IOCB Prague: Research from IOCB Prague reveals a previously unknown mechanism of genetic transcription: This work shows how NpnNs initiate bacterial transcription. Cryo-EM reveals how nucleobases pair with the template: one in canonical and one in reverse Watson–Crick mode, providing a structural basis for their function as non-canonical RNA caps." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_UOCHB_Vyzkum_z_UOCHB_odhaluje_dosud_nepoznany_zpusob_prepisu_geneticke_informace_3_4d881e7a58.jpg 245w," sizes="100vw" width="2000" height="1077"><blockquote><h4>The Original article</h4><h4><a target="_blank" rel="noopener noreferrer" href="https://www.nature.com/articles/s41589-025-02134-5">Molecular insight into 5′ RNA capping with NpnNs by bacterial RNA polymerase</a></h4>Valentina M. Serianni, Jana Škerlová, Anna Knopp Dubánková, Anton Škríba, Hana Šváchová, Tereza Vučková, Anatolij Filimoněnko, Milan Fábry, Pavlína Řezáčová, Tomáš Kouba &amp; Hana Cahova&nbsp;Nat. Chem. Biol. 21 (2026)<a target="_blank" rel="noopener noreferrer" href="https://doi.org/10.1038/s41589-025-02134-5">https://doi.org/10.1038/s41589-025-02134-5</a><h6>licensed under <a target="_blank" rel="noopener noreferrer" href="https://creativecommons.org/licenses/by/4.0/">CC-BY 4.0</a></h6></blockquote><h4>Abstract</h4>RNA capped with dinucleoside polyphosphates has been discovered in bacteria and eukaryotes only recently. The likely mechanism of this specific capping involves direct incorporation of dinucleoside polyphosphates by RNA polymerase as noncanonical initiating nucleotides. However, how these compounds bind into the active site of RNA polymerase during transcription initiation is unknown. Here, we explored transcription initiation in vitro, using a series of DNA templates in combination with dinucleoside polyphosphates and model RNA polymerase from Thermus thermophilus. We observed that the transcription start site can vary on the basis of the compatibility of the specific template and dinucleoside polyphosphate. Cryo-electron microscopy structures of transcription initiation complexes with dinucleoside polyphosphates revealed that both nucleobase moieties can pair with the DNA template. The first encoded nucleotide pairs in a canonical Watson–Crick manner, whereas the second nucleobase pairs noncanonically in a reverse Watson–Crick manner. Our work provides a structural explanation of how dinucleoside polyphosphates initiate RNA transcription.</div></div>

Research from IOCB Prague reveals a previously unknown mechanism of genetic transcription

Food adulteration is a phenomenon with a long history that has gained increasing importance in recent years due to the globalization of food supply chains and strong economic pressures. Whether it involves diluting olive oil with cheaper oils, adding sugar to honey, or the hidden use of substitutes in protein supplements, the result is a loss of consumer trust and, in some cases, a potential risk to health. Analytical chemistry plays a key role in this area by providing methods and technologies capable of identifying and quantifying target compounds as well as unwanted impurities in a wide range of food matrices.<img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Watrex_Praha_Falsovani_potravin_a_jak_ho_odhali_analyticka_chemie_6912db5492.jpg" alt="Watrex: Food Adulteration and How It Is Revealed by Analytical Chemistry" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Watrex_Praha_Falsovani_potravin_a_jak_ho_odhali_analyticka_chemie_6912db5492.jpg 245w," sizes="100vw" width="1600" height="900"><h4>The Role of Chromatography in Forensic Food Analysis</h4>Chromatography, particularly liquid chromatography (HPLC), is one of the most effective analytical tools for separating complex mixtures of molecules. It enables:<ul><li>qualitative identification of individual components in a sample,</li><li>quantitative determination of their concentrations,</li><li>detection of impurities and unexpected components that may indicate adulteration.</li></ul>In practice, HPLC is often combined with various types of detectors (UV/VIS, DAD, refractive index detectors) or with other techniques (e.g., mass spectrometry) to increase selectivity and sensitivity.<h4>Isocratic vs. Gradient Elution</h4>In the analysis of food samples, chromatographic methods are selected according to the analytical goal:<ul><li>Isocratic HPLC uses a constant mobile phase composition throughout the analysis. It is simple, robust, and ideal for routine determinations of well-defined target analytes (e.g., sugars or vitamins).</li><li>Gradient HPLC changes the mobile phase composition during separation, enabling better resolution of complex mixtures with a wide range of polarities (e.g., peptides or phenolic compounds).</li></ul>Gradient methods are particularly useful when analytes have very different properties and high separation efficiency is required.<h4>Watrex Praha and the Contribution of Modern Systems to Food Analysis</h4><a target="_blank" rel="noopener noreferrer" href="https://watrex.com/?utm_source=labrulez&amp;utm_medium=propagace&amp;utm_campaign=labrulez_clanky_HPLCsystemy">Watrex Praha, s.r.o.</a> is one of the leading Czech manufacturers and distributors of liquid chromatography systems and instruments, with a long tradition dating back to 1990. The company has successfully transformed from a distributor into a manufacturer of its own HPLC and related technologies, focusing on modular, robust systems suitable even for demanding analytical tasks.Their <a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/products?manufacturerItems=93&amp;search=streamline">Streamline™ product line</a> includes several HPLC system concepts suitable for food analysis:<ul><li>Isocratic HPLC system – a simple yet precise configuration with a single pump, column thermostat, and UV/VIS detector; suitable for routine determination of known analytes (e.g., basic carbohydrates, food additives).</li><li>Binary gradient HPLC system – equipped with two pumps enabling mobile phase gradient formation, which enhances separation performance for complex mixtures (e.g., phenolic profiles of wine, components of aromatic extracts).</li><li>GPC/SEC system (Gel Permeation / Size Exclusion Chromatography) – a specialized solution for the determination of macromolecules and polymers, also relevant for the analysis of high-molecular-weight components in foods (e.g., polysaccharides).</li></ul><blockquote>👉<a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/labrulez-bucket-strapi-h3hsga3/Watrex_catalog_2024_ba97395f92.pdf">Product catalogue HERE</a></blockquote>Thanks to the modular nature of these systems, analysts can easily adapt the configuration to a specific analytical problem, from simple routine analyses to complex characterization of food samples.<h4>Practical Examples of Adulteration Revealed by HPLC</h4><ul><li>Detection of olive oil dilution: HPLC can separate and quantify characteristic triacylglycerols and fatty acids; unusual patterns indicate the presence of cheaper oils.</li><li>Estimation of sugar addition in honey: Isocratic HPLC with a refractive index detector can determine sugar profiles (glucose, fructose, sucrose), revealing dilution or the addition of sugar syrups.</li><li>Identification of aromatic adulterants in wine: Gradient HPLC with a PDA detector can reveal phenolic and aromatic profiles that are altered by the addition of synthetic compounds.</li></ul><h4>Conclusion</h4>Food adulteration remains a current issue that requires reliable and sensitive analytical methods. Liquid chromatography, and in particular modern HPLC systems, represent one of the most powerful tools for detecting unwanted additives, impurities, and unexpected components in complex food samples. Manufacturers such as <a target="_blank" rel="noopener noreferrer" href="https://watrex.com/?utm_source=labrulez&amp;utm_medium=propagace&amp;utm_campaign=labrulez_clanky_HPLCsystemy">Watrex Praha</a> provide flexible chromatographic platforms covering a wide range of analytical tasks from simple isocratic to advanced gradient and GPC/SEC applications thereby supporting analysts in ensuring food quality and consumer protection.<blockquote>Did you find this article interesting? Follow <a target="_blank" rel="noopener noreferrer" href="https://watrex.com/?utm_source=labrulez&amp;utm_medium=propagace&amp;utm_campaign=labrulez_clanky_HPLCsystemy">Watrex Praha</a> on their social media (<a target="_blank" rel="noopener noreferrer" href="https://www.linkedin.com/company/watrex/?viewAsMember=true">LinkedIn</a> and <a target="_blank" rel="noopener noreferrer" href="https://www.facebook.com/WatrexPrague">Facebook</a>) for more engaging topics.</blockquote>

Food Adulteration and How It Is Revealed by Analytical Chemistry

Join us for a fascinating conversation with Dr. Subhoja Chakraborty from the University of Central Florida, whose cutting-edge research targets the global challenge of malaria drug resistance. Dr. Chakraborty shares her journey from structure-based drug design during her PhD to her recent collaborative work repurposing human kinase inhibitors as rapid, selective antimalarials against drug-resistant Plasmodium falciparum strains.<h4>In this episode, you'll learn:</h4><ul><li>How structure-guided approaches reveal potential drug targets, including cysteine proteases like falcipain-2, and kinases implicated in malaria's complex life cycle.</li><li>The power of repurposing kinase inhibitors originally developed for cancer and other diseases, focusing on compound 12, which blocks hemozoin formation and protein kinase 6—key steps in parasite survival.</li><li>Lab techniques such as affinity, gel filtration, and size exclusion chromatography for recombinant protein purification—and how troubleshooting is crucial for high-yield, stable complexes.</li><li>Advances in mass spectrometry for characterizing protein interactions, validating drug targets, and mapping molecular pathways pivotal to malaria therapeutics.</li><li>Tips and lessons for new researchers facing the challenges of sample preparation, complex protocols, and working as part of a collaborative team.</li></ul>Dr. Chakraborty’s insights bridge the gap between basic science and translational drug discovery, offering hope for more effective, targeted malaria treatments in a world where resistance to frontline therapies threatens millions.<h4>Referenced Papers:</h4><ul><li>"Plasmodium falciparum protein kinase 6 and hemozoin formation are inhibited by a type II human kinase inhibitor exhibiting antimalarial activity" (Cell Chemical Biology, 2025)</li><li>"New insights of falcipain 2 structure from Plasmodium falciparum 3D7 strain" (BBRC, 2022)</li><li>"Structure-Based Optimization of Protease−Inhibitor Interactions to Enhance Specificity of Human Stefin‑A against Falcipain‑2 from the Plasmodium falciparum 3D7 Strain" (Biochemistry, 2023)</li></ul>If you’re a student, scientist, or simply passionate about advances in infectious disease research, this episode offers actionable insights, inspiring advice, and a front-row seat to innovation in combating malaria.<h3>Video Transcription</h3><h4>From Structural Biology to Drug Discovery: Chromatography and Mass Spectrometry in Malaria Research</h4><h5>Introduction</h5>In this discussion, Subhoja shares insights into her research journey, focusing on malaria drug discovery and the role of modern analytical techniques in biological and biomedical research. The conversation is aimed primarily at undergraduate students and early-career researchers interested in chemistry, biotechnology, and life sciences.<h5>Research Focus: Malaria Drug Discovery and Kinase Targets</h5>Subhoja is currently involved in malaria drug discovery research at the University of Central Florida, where she has been working since beginning her postdoctoral position two years ago. Her earlier PhD research focused on structure-based drug design in malaria, using a combination of in silico approaches, recombinant protein expression, and X-ray crystallography to determine precise three-dimensional structures of target proteins and their inhibitors.This structural perspective provided a foundation for her current work, which centers on drug discovery through compound screening and repurposing. A recent publication from her laboratory explores the use of repurposed mammalian CDK-like kinase inhibitors as potential antimalarial agents. While such kinase inhibitors are well known in cancer and cardiovascular research, their application to malaria targets represents a novel and promising approach.Kinases are attractive drug targets because of their central role in cell signaling and growth pathways. By targeting specific kinases involved in different stages of the malaria parasite life cycle—whether asexual growth, gametocytogenesis, or differentiation—it may be possible to significantly inhibit parasite growth and progression.<h5>Chromatography in Protein Expression and Purification Workflows</h5>Chromatography plays a critical role throughout Subhoja’s research. Her experience spans from basic chromatography techniques during her early training to advanced protein purification workflows using affinity chromatography, gel filtration (size exclusion chromatography), and ion exchange chromatography.Recombinant proteins are routinely purified using affinity resins such as nickel or cobalt, followed by further purification to remove contaminants. Fast Protein Liquid Chromatography (FPLC) enables compact, stepwise purification workflows suitable for producing proteins for in vitro assays.A recurring challenge in this process is the expression of high-molecular-weight proteins, which often accumulate in insoluble fractions or inclusion bodies. Careful selection of expression vectors, host systems, and induction conditions is therefore essential to maximize yield and solubility.<h5>Integrating Size Exclusion Chromatography into the Workflow</h5>Size exclusion chromatography (SEC) is typically used after affinity purification to further improve protein purity. Following elution with imidazole or glutathione, desalting is required to prevent buffer components from interfering with downstream assays. This is often achieved using desalting columns such as G-25 or PD-10.To remove lower-molecular-weight contaminants, finer SEC media—such as Superdex or Sephacryl columns—are employed. Concentration steps using molecular-weight cut-off filters are then applied to obtain the desired protein concentration for functional studies.These iterative purification and troubleshooting steps are a routine part of protein biochemistry and reflect the importance of methodical optimization and careful validation.<h5>The Role of Mass Spectrometry in Biomolecular Characterization</h5>Mass spectrometry is a key analytical tool in Subhoja’s research pipeline, particularly for target identification and validation. In both malaria and cancer research, MS is used alongside chromatography, immunoprecipitation, and pull-down assays to study protein interactions and molecular recognition events.Affinity-based mass spectrometry enables the identification of binding partners and interactors, whether studying proteins, long non-coding RNAs, or protein complexes. Techniques such as native MS, tandem affinity purification, and proximity labeling (e.g., TurboID-based workflows) provide deeper insights into molecular networks and signaling pathways.These approaches are essential for understanding how candidate drug targets function within complex biological systems.<h5>Sample Preparation Challenges in Biological Research</h5>Sample preparation remains one of the most challenging aspects of biological experimentation. Whole-cell lysates contain proteases, nucleases, and other components that can rapidly degrade target molecules. Preventing degradation requires minimizing preparation time, maintaining low temperatures, and using appropriate inhibitor cocktails.Reproducibility is also critical: biological experiments require multiple replicates, and variability is often unavoidable. Careful handling, protocol optimization, and continuous troubleshooting are essential to ensure reliable results, whether preparing samples for chromatography, mass spectrometry, sequencing, or immunoprecipitation.<h5>Advances in Chromatography and MS for Malaria Research</h5>Modern advances in chromatography and mass spectrometry significantly enhance malaria research by enabling precise target identification, quantification, and functional characterization. Drug discovery workflows begin with construct design and recombinant expression, followed by purification and interaction studies.In malaria research, stage-specific parasite biology requires careful extraction and analysis of proteins from different life cycle stages. Tandem affinity MS, immunoprecipitation, and in vivo validation studies help confirm target relevance and therapeutic potential.Recent work on repurposed kinase inhibitors has shown promising results, including inhibition of hemozoin formation—a key detoxification pathway for the malaria parasite—highlighting the potential of such compounds for further development.<h5>Malaria, Drug Resistance, and Global Impact</h5>Malaria remains a major global health burden, with over 600,000 deaths annually and widespread drug resistance complicating treatment. Artemisinin resistance, particularly associated with mutations in the K13 propeller domain, has driven the search for new targets and therapies.During her PhD, Subhoja focused on falcipain-2, a cysteine protease involved in hemoglobin degradation within the parasite. This pathway is critical for parasite survival and has emerged as a promising drug target. Her work revealed that falcipain-2 operates within a larger protein complex responsible for hemoglobin processing and amino acid release.Understanding these molecular mechanisms is essential for developing robust, stage-specific antimalarial strategies.<h5>Advice for Early-Career Researchers</h5>For researchers just starting out, Subhoja emphasizes the importance of hands-on experience. Working directly with cells, lysates, and analytical tools is essential for truly understanding experimental systems. Failure is an inherent part of research, and perseverance is key.Motivation, teamwork, careful sample handling, and thorough preparation all contribute to long-term success. Reading extensively, critically evaluating one’s own work, and appreciating incremental progress are vital aspects of a sustainable research career.Every successful experiment—no matter how small—represents meaningful progress and contributes to the broader goals of science and medicine.<h5>Closing Remarks</h5>The discussion highlights how interdisciplinary approaches combining chromatography, mass spectrometry, structural biology, and biochemistry are driving advances in malaria drug discovery. Through careful experimental design, analytical rigor, and collaborative teamwork, researchers can address complex biological challenges and contribute to global health solutions.<blockquote>This text has been automatically transcribed from a video presentation using AI technology. It may contain inaccuracies and is not guaranteed to be 100% correct.</blockquote><blockquote><h4>Concentrating on Chromatography Podcast</h4>Dive into the frontiers of chromatography, mass spectrometry, and sample preparation with host David Oliva. Each episode features candid conversations with leading researchers, industry innovators, and passionate scientists who are shaping the future of analytical chemistry. From decoding PFAS detection challenges to exploring the latest in AI-assisted liquid chromatography, this show uncovers practical workflows, sustainability breakthroughs, and the real-world impact of separation science. Whether you’re a chromatographer, lab professional, or researcher you'll discover inspiring content!You can find Concentrating on Chromatography Podcast in podcast apps:<ul><li><a target="_blank" rel="noopener noreferrer" href="https://open.spotify.com/show/1wZg67rCjoGju4yhqxI3tL">Spotify</a></li><li><a target="_blank" rel="noopener noreferrer" href="https://podcasts.apple.com/tz/podcast/concentrating-on-chromatography/id1788429185">Apple podcast</a></li><li><a target="_blank" rel="noopener noreferrer" href="https://podscan.fm/podcasts/concentrating-on-chromatography">podscan</a></li></ul>and on <a target="_blank" rel="noopener noreferrer" href="https://www.youtube.com/@ChromatographyTalk/videos">YouTube channel</a></blockquote>

Affinity, Gel Filtration, Size Exclusion Chromatography: Repurposing Kinase Inhibitors for Malaria

Article

Product

Video

Scientists

Food

Interview

Science and research

The Institute of Organic Chemistry and Biochemistry of the Czech Academy of Sciences (IOCB Prague) is a leading scientific institution in the Czech Republic, recognized internationally. Its primary mission is basic research in the fields of chemical biology and medicinal chemistry, organic and material oriented chemistry, chemistry of natural compounds, biochemistry and molecular biology, physical chemistry, theoretical chemistry, and analytical chemistry. 

Institute of Organic Chemistry and Biochemistry of the Czech Academy of Sciences

Organomation, founded in 1959, designs and manufactures high-quality nitrogen evaporators and extraction systems. Known for innovation and durability, their lab instruments are used globally for efficient sample preparation with strong customer support.

Organomation

WATREX Prague is the distributor and producer of HPLC techniques in the Czech Republic and Slovakia. Main Chromatografic and Related Techniques Supported: LC, GPC, IC, IEC, Automated SPE, LC Columns and special column packings, LC method development, Software development, Laboratory Robots.

Watrex Praha

Watrex Streamline RI1 Refractive index HPLC detector

The Watrex Streamline RI1 Refractive Index Detector is an essential component in high-performance liquid chromatography (HPLC) systems.

Streamline™ RI1 refractive index detector is suitable for detecting compounds with little or no UV activity such as alcohols, sugars, lipids or polymers. This instrument is designed for use in analytical HPLC (high performance liquid chromatography) as well as for GPC (gel permeation chromatography) applications.<h3>FEATURES</h3><ul><li>Advanced temperature control for high sensitivity and reproducibility</li><li>Prime purge valve</li><li>Long-life LED light source</li><li>Pressure resistant flow cell</li></ul><h3>TECHNICAL SPECIFICATION</h3><ul><li>Light Source: Long-life LED</li><li>Refractive index Range: 1.00 - 1.75 RIU</li><li>Baseline Noise: ± 2.5 nRIU</li><li>Baseline Drift: 200 nRIU/h</li><li>Linearity: &gt; 1000 μRIU</li><li>Flow cell pressure resistance: 5 bar</li><li>Flow cell volume: 15 μl</li><li>Time constant: 0.00/0.01/0.02/0.05/0.1/0.2/0.5/1.0/2.0/5.0/10.0 s</li><li>Analog Output: 1x 0-2.5V</li><li>Outputs: Event 1, Start (OUT), Error (OUT), + 5 V, 24 V Valve</li></ul><h3>Ordering information:</h3><h5>PN/Component:</h5><ul><li>SRI101-001 Watrex Streamline™ RI refractive index detector (Price 7 018 € ex VAT)</li></ul>You can also visit this product on the <a target="_blank" rel="noopener noreferrer" href="https://watrex.com/obchod/chromatography/detectors/other-hplc-detectors/streamline-ri1-detector/?utm_source=labrulez&amp;utm_medium=propagace&amp;utm_campaign=labrulez_produkty">Watrex website</a>.

Watrex Streamline VD1 Viscosimetric HPLC detector

 Watrex Streamline™ VD1 viscosimetric detector is designed for detailed characterization of polymer compounds in both aqueous and organic solvents.

Streamline™ VD1 viscosimetric detector measures absolute intrinsic viscosity and is ideal as a detector for online monitoring in any GPC system. VD1 enables measurement of absolute molecular size using Wheatstone bridge operating principle.<h3>FEATURES</h3><ul><li>High sensitivity and stability</li><li>Low-noise</li><li>Open protocol digital data format</li><li>Direct dual analog output</li></ul><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Watrex_Watrex_Streamline_VD_1_Viscosimetric_detector_Universal_calibration_from_VD_1_data_1_149c741933.jpg" alt="Watrex: Watrex Streamline VD1 Viscosimetric detector - Universal calibration from VD1 data 1"><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Watrex_Watrex_Streamline_VD_1_Viscosimetric_detector_Universal_calibration_from_VD_1_data_2_a616c498e8.jpg" alt="Watrex: Watrex Streamline VD1 Viscosimetric detector - Universal calibration from VD1 data 2"><h3>TECHNICAL SPECIFICATION</h3><ul><li>Wavelength Range: 190 – 720 nm</li><li>Baseline Noise: ± 1 x 10-5 AU (@240 nm, 2 sec. risetime)</li><li>Baseline Drift: &lt; 3 x 10-4 AU/h</li><li>Wavelength Accuracy: ± 0.5 nm</li><li>Wavelenghth Reproducibility: ± 0.1 nm</li><li>Mean Pixel Pitch: 2.2 nm</li><li>Resolution (λ FWHM): 7 nm</li><li>Linearity: &gt; 2.5 AU</li><li>Wavelength Program: Programmable, 10 steps</li><li>Analog Output (optional): 1x 1 V</li><li>Options: Dual channel configuration, thermostated flow cell</li></ul><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Watrex_Watrex_Streamline_VD_1_Viscosimetric_detector_Technical_specification_76756715f6.jpg" alt="Watrex: Watrex Streamline VD1 Viscosimetric detector - Technical specification"><h3>Ordering information:</h3><h5>PN/Component:</h5><ul><li>SVD101-001 Watrex Streamline™ VD1 viscosimetric detector (Price 19 741 € ex VAT)</li></ul>You can also visit this product on the <a target="_blank" rel="noopener noreferrer" href="https://watrex.com/obchod/chromatography/detectors/other-hplc-detectors/streamline-vd1-detector/?utm_source=labrulez&amp;utm_medium=propagace&amp;utm_campaign=labrulez_produkty">Watrex website</a>.

SIELC Alltesta Mini UV-Vis Detector

The Alltesta™ UV-Vis Multi-wavelength Mini Detector is a compact and versatile device that can continuously monitor the optical properties of solutions flowing through it.

The Alltesta™ UV-Vis Multi-wavelength Mini Detector is a compact and versatile device that can continuously monitor the optical properties of solutions flowing through it. The Mini Detector is ideal for use in HPLC, Flow Injection Analysis (FIA), and preparative and flash chromatography.
The device comes standard with four different wavelength channels, including 470 nm (Blue), 520 nm (Green), 630 nm (Red), and a choice between 255 nm or 275 nm for the UV channel. The Mini Detector features a simple setup process with a USB interface that allows it to communicate with the <a href="https://lcms.labrulez.com/products/1242">Alltesta™ Gradient Automated Analyzer</a> or <a href="https://lcms.labrulez.com/products/1243">Alltesta™ Cromite Analyzer</a> or integrate with other instrumentation.
Powered solely by its USB port, this mini detector is capable of monitoring multiple analytes simultaneously. It has a low optical cell volume of &lt; 3 µL, which results in minimal dispersion even at low flow rates, and can collect data at rates of up to 20 sps for all four channels, making it suitable for demanding applications. The Mini Detector boasts low noise and linearity over more than 4 orders of magnitude.
In addition to its compact size and versatility, the Mini Detector also has zero warm-up time and exceptional durability. The detector cell volume is available in two sizes: 3 μL and 10 μL.
<h4>Specifications</h4>
<ul>
<li>Dimensions (WHL): 63 x 46 x 116 mm / 2.48 x 1.81 x 4.57 in</li>
<li>Weight: 0.25 kg / 0.55 lb</li>
<li>Sample Cell Volume: &lt; 3µL</li>
<li>Cell Optical Path: 7.0mm</li>
<li>Liquid Ports: 10-32 UNF</li>
<li>Wavelength Range: 250nm &amp; up</li>
<li>Simultaneous Wavelengths: Up to 4</li>
<li>Data Rate: 20 sps</li>
<li>Noise Level: 0.03 mAU</li>
<li>Power: USB Type B</li>
</ul>
<h4>Software</h4>
The Multi-wavelength Detector can be controlled:
<ul>
<li>directly via serial commands (Alltesta™ protocol available upon request, please contact us at support@sielc.com),</li>
<li>directly via our demo software, allowing to learn the whole functionality of the unit (<a href="https://sielc.com/wp-content/uploads/2022/12/Alltesta_Detector_Control_Windows_10.zip">Windows 10, 32/64bit</a>, 5Mb, <a href="https://sielc.com/wp-content/uploads/2022/12/Alltesta_Detector_Control_Windows_7.zip">Windows 7, 32/64bit</a>, 127Mb)</li>
<li>remotely through our cloud software (as a part of <a href="https://lcms.labrulez.com/products/1242">Alltesta™ Gradient Automated Analyzer</a> and <a href="https://lcms.labrulez.com/products/1243">Alltesta™ Cromite Analyzer</a>).</li>
</ul>
Serial Communication Drivers are available <a href="https://ftdichip.com/drivers/vcp-drivers/">here</a>.

Waters/Wyatt microViscoStar viscometer

The Waters microViscoStar incorporates multiple novel technologies to provide the highest sensitivity, stability and solvent compatibility of any available viscometer for UHPLC. 

<h4>Highly Sensitive</h4>A highly sensitive, on-line differential viscometer used with SEC-MALS-IV to determine the size and conformation of polymers, proteins and peptides separated by UHPLC.<h4>Multiple Novel Technologies</h4>The microViscoStar™ incorporates multiple novel technologies to provide the highest sensitivity, stability and solvent compatibility of any available viscometer for UHPLC. Its ease-of-use and serviceability make it the perfect companion for Wyatt's microDAWN™ light scattering and microOptilab™ refractive index detectors for UHPLC.<h4>Another path to polymer and protein characterization: SEC-MALS-IV</h4>When coupled with size-exclusion chromatography and a concentration detector such as the Optilab™ , the microViscoStar derives the intrinsic viscosity (IV) of polymers. An inherently valuable physico-chemical property in itself, IV is best utilized together with molar mass information from multi-angle light scattering (SEC-MALS-IV) to derive the hydrodynamic radius, conformation, and branching ratio of a macromolecule in solution.<img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Waters_Visco_Star_pump_pulse_suppression_with_inset_e8c2fbfc18.jpg" alt="Waters microViscoStar: Patented pulse compensation technology in the microViscoStar eliminates pump fluctuations. At the hardware level, the bridge is impedance-matched for dynamic balance, greatly reducing the effect of pump pulses. An additional layer of software filtering eliminates residual pump pulses." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Waters_Visco_Star_pump_pulse_suppression_with_inset_e8c2fbfc18.jpg 233w," sizes="100vw" width="348" height="233"><h5>Key benefits of the ViscoStar</h5><ul><li>High sensitivity, equivalent to 5 ng of 100 kDa polystyrene in THF at 1.0 mL/min injected onto a standard GPC column</li><li>High dynamic range of over 135,000:1. The full measurement range corresponds to over 13 mg/mL of 100 kDa polystyrene in THF</li><li>Extended solvent compatibility with all-316 stainless steel transducers</li><li>Temperature range from 4 °C to 70 °C for polymers or proteins that require a range of temperatures</li><li>Minimal band broadening thanks to fast pressure transducers</li><li>Pump pulse suppression to below 4 Pa (hardware alone) or 1 Pa (hardware + software) without loss of chromatographic resolution</li><li>Automated capillary bridge tuning with no moving parts, to guarantee a perfectly balanced bridge, every run</li><li>Enhanced thermal stability with drift of &lt;1.25 Pa/hour</li></ul><h4>SEC-IV without MALS?</h4>Yes, as much as we love MALS for optimal polymer analysis, it's true that there are occasions when it just won't fit the bill.Universal calibration is a parallel technique to SEC-MALS for determining molecular weight, commonly used to characterize linear polymers. While universal calibration overcomes some of the problematic issues associated with column calibration by reference molecules, it does not account for non-ideal column behavior caused by chemical solute-column interactions or hyperbranching.Universal calibration is particularly-well suited to replace MALS for:<ul><li>Strongly fluorescent polymers</li><li>Weakly-scattering polymer/solvent systems with very low dn/dc values</li></ul>The Mark-Houwink-Sakurada relationship makes use of empirical relationships between intrinsic viscosity and molar mass, specific to each polymer and solvent, to estimate molar mass from SEC-IV measurements. Of course, the best way to determine MHS coefficients is to measure them using SEC-MALS-IV and ASTRA™<h4>Specifications</h4><h5>Measurement Capabilities – Differential Viscosity</h5><ul><li>Sensitivity: Detects as little as 5 ng of 100 kDa polystyrene in THF at 1 mL/min under typical UHPLC-SEC conditions.</li><li>Differential Pressure Performance:<ul><li>Noise: &lt; 0.05 Pa</li><li>Range: Up to 6.9 kPa, with overpressure protection</li><li>Drift: &lt; 1.25 Pa/hr</li><li>Linearity: Within 0.5% of full scale</li><li>Dynamic Range: 135,000:1</li></ul></li><li>Capillary Bridge Autobalance: Thermal, leak-free mechanism ensures stable baseline performance.</li><li>Sample Shear Rate: Up to 15,000 Hz with water at 1 mL/min for high-resolution viscosity profiling.</li></ul><h5>Fluidics and Compatibility</h5><ul><li>Maximum Flow Rate: Aqueous and THF: up to 1 mL/min (Note: Using an inline UV detector may reduce allowable flow rate.)</li><li>Delay Volume: 5.4 mL</li><li>Maximum Inlet-to-Outlet Pressure: 3000 kPa (435 psi)</li><li>Wetted Materials: 316 stainless steel, PTFE, zirconium silicate, Kalrez®, Inconel, 26-1 stainless steel, and nickel</li></ul><blockquote>Note: Due to the location of wetted components, this instrument is primarily recommended for use with organic solvents. High-ionic-strength buffers may cause corrosion.</blockquote><h5>Temperature Control</h5><ul><li>Operating Range: 4 °C to 70 °C</li><li>Stability: Maintains temperature within &lt; 0.005 °C</li><li>Ambient Conditions: 0 °C to 40 °C; 20% to 70% relative humidity</li></ul><h5>Electronics and Connectivity</h5><ul><li>Digital Output: 20-bit resolution at 10 Hz</li><li>Auxiliary I/O:<ul><li>Inputs: Autoinject contact closure, Alarm In, and 2 analog inputs (16-bit, ±10 V)</li><li>Outputs: Autoinject retransmit, Alarm Out, Recycle Out</li></ul></li><li>Communication Interface: Ethernet</li></ul><h5>User Interface</h5><ul><li>Front Panel Display: 7” (178 mm) high-resolution, capacitive multi-touch screen for real-time monitoring, configuration, and diagnostics</li></ul><h5>Instrument Dimensions</h5><ul><li>Size: 60 cm (L) × 36 cm (W) × 17 cm (H)</li></ul><h4>Other Viscometers</h4><ul><li><a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/products/1117">ViscoStar™</a> - A highly sensitive, on-line differential viscometer used in conjunction with SEC-MALS to determine the size and conformation of all types of biopolymers, synthetic polymers and even proteins and peptides.</li><li>The ViscoStar incorporates multiple novel technologies to provide the highest sensitivity, stability and solvent compatibility of any available viscometer for GPC. Its ease-of-use and serviceability make it the perfect companion for Wyatt's DAWN® light scattering and Optilab® refractive index detectors. Temperature-controlled from 4 °C to 70 °C .</li></ul>

Waters/Wyatt Optilab On-line Differential Refractometer

The Waters Optilab is a unique dRI detector for standard HPLC/GPC with 256 times the detection power and 50 times the dynamic range of every other RI detector for chromatography in existence today, making it a valuable addition to any MALS setup.

<h4>Independent of Chromaphores</h4>Independent of chromaphores or fluorophores, differential Refractive Index (dRI) detectors serve as accurate and versatile concentration detectors in all types of solvents. dRI is part of standard instrumentation for a variety of macromolecular characterization techniques including standard chromatography, SEC-MALS, FFF-MALS and CG-MALS.<h4>Unique dRI Detector</h4>The Optilab is a unique dRI detector for standard HPLC/GPC with 256 times the detection power and 50 times the dynamic range of every other RI detector for chromatography in existence today, making it a valuable addition to any MALS setup.<h4>Product Description</h4>Using a combination of cutting-edge semiconductor photodiode technology and proprietary computer algorithms, the Optilab achieves an unprecedented combination of sensitivity and range. These features mean that it addresses both standard chromatographic applications and some challenges unique to light scattering measurements such as high concentrations, determination of sample refractive increments (dn/dc) and solvent refractive index.<h5>Key Benefits</h5><ul><li>Extraordinary dRI measurement range, -0.0047 to +0.0047 RIU (comparable to 25 mg/mL protein in PBS)</li><li>Highly sensitive, ±7.5 x 10 -10 &nbsp;RIU across the entire measurement range</li><li>Measures solvent absolute refractive index, required for MALS calculations</li><li>Measures at the same wavelength as the MALS detector, for maximum accuracy in MALS calculations</li><li>Band broadening &lt; 20 µL preserves SEC resolution</li><li>Temperature range 4 °C to 65 °C for proteins and polymers that require a range of temperatures</li><li>High-resolution, touch-screen front panel displays data graphs and diagnostics, even with the host computer turned off</li></ul><h5>Unparalleled Features</h5>Even though the Optilab offers a dRI measurement range orders of magnitude beyond that of other dRI detectors, unlike other detectors there are no range or gain settings in the Optilab. The full range of instrument detection is always present, and the full sensitivity exists over the entire range.The Optilab can also do something no other on-line refractive index detector can: it can measure the absolute refractive index of a solution. In addition, it can measure the dn/dc of a solvent at the same wavelength of light as the light scattering instrument. And just to make sure it can't be matched, you can replace the light source in the Optilab for other wavelengths (e.g., 690, 633, 488, etc.) in just minutes!The Optilab can go below ambient temperatures as easily as above ambient. No hassle temperature control can be programmed from 4 °C to 65 °C. Compatible with all HPLC systems.<h4>Specifications</h4><h5>Measurement Capabilities – Differential Refractive Index</h5>Standard Optilab Configuration<ul><li>Measurement Range: –0.0047 to +0.0047 RIU</li><li>Peak-to-Peak Noise: ±7.5 × 10⁻¹⁰ RIU (Measured per ASTM-E1303-95 at 25 °C)</li></ul>High Concentration (HC) Option<ul><li>Measurement Range: –0.0026 to +0.034 RIU</li><li>Peak-to-Peak Noise: ±1.5 × 10⁻⁹ RIU (Optimized for higher concentration samples)</li><li>Response Time: User-selectable from 0.1 to 5 seconds</li><li>Absolute Refractive Index Measurement Range: 1.2 to 1.8 RIU</li><li>Absolute RI Sensitivity: ±0.002 RIU</li></ul>Fluidics and Flow Properties<ul><li>Band Broadening: &lt;12 µL</li><li>Fill Volume (Inlet Tubing + Flow Cell): 51.4 µL</li><li>Outlet Tubing Volume (0.030" ID): 370 µL</li></ul>Pressure Ratings<ul><li>Inlet Back Pressure (0.5 mL/min H₂O @ 20 °C): &lt;22 psi (1.5 bar)</li><li>Max Outlet Back Pressure: 30 psi (2 bar)</li></ul>Temperature Control<ul><li>Sample Temperature Control Range: 4 °C to 65 °C</li><li>Regulation Stability: ±0.005 °C</li></ul>Light Source Options<ul><li>Standard: 660 nm</li><li>HC Model (Optional): 488 nm, 785 nm, or 940 nm</li></ul>Safety Features<ul><li>Built-in vapor and liquid leak sensors for enhanced lab safety</li></ul>Electronics and Connectivity<ul><li>Digital Output: 22+ bit resolution @ 10 Hz</li><li>Analog Output: ±10 V or ±1 V, 18-bit resolution</li><li>Computer Interface: Ethernet</li></ul>Auxiliary I/O Ports<ul><li>Inputs:<ul><li>Analog In (16-bit, ±10 V A/D)</li><li>Zero dRI</li><li>Purge</li><li>Recycle In</li><li>Autoinject In</li><li>Alarm In</li></ul></li><li>Outputs:<ul><li>Autoinject Retransmit</li><li>Alarm Out</li><li>Recycle Out (controls Orbit™ valve switching)</li></ul></li></ul>User Interface<ul><li>Front Panel Display: 7” (178 mm) high-resolution multi-touch screen for viewing signals, diagnostics, and adjusting instrument settings</li></ul>Instrument Dimensions<ul><li>Size: 58 cm (L) × 36 cm (W) × 18 cm (H)</li></ul><h4>Other Refractive Index Detectors</h4><ul><li><a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/products/1269">microOptilab™</a> - The first RI detector specifically designed for use with all UHPLC systems.</li></ul>

Waters/Wyatt microOptilab On-line Differential Refractometer

The Waters microOptilab brings the universal detection capabilities, unsurpassed concentration range, and superb sensitivity and linearity

<h4>Independent of Chromaphores</h4>Independent of chromaphores or fluorophores, differential Refractive Index (dRI) detectors measure concentrations of all types of samples in almost all solvents. The microOptilab brings the universal detection capabilities, unsurpassed concentration range, and superb sensitivity and linearity – familiar from the Optilab™ – to the domain of UHPLC.<h4>SEC-MALS with UHPLC</h4>Like standard SEC, UHPLC-SEC analysis relying on column calibration with molecular standards is unreliable when the standards do not accurately reflect your samples in terms of conformation, non-ideal (e.g., hydrophobic) column interactions or reversible association. SEC-MALS employs a microDAWN™ MALS detector downstream of the SEC column and UV detector, to determine molar mass / molecular weight distributions of macromolecules independently of column calibration.Refractive index detection is an important component of any SEC-MALS system, and necessary for analyzing conjugated proteins or polymers devoid of chromaphores. The microOptilab connects with the microDAWN MALS detector for a complete UHP-SEC-MALS setup that transfers the many benefits of SEC-MALS analysis to the realm of UHPLC.<h5>Key benefits</h5><ul><li>Very low band broadening, &lt; 4 µL, to maintain the resolution of very narrow UHPLC peaks</li><li>Extraordinary dRI measurement range, -4.7 x 10 -3 &nbsp;to +4.7 x 10 -3 &nbsp;RIU (comparable to 25 mg/mL protein in PBS)</li><li>Highly sensitive, ± 1.5 x 10 -9 &nbsp;RIU across the entire measurement range</li><li>Measures solvent refractive index, require for MALS calculations</li><li>Measures at the same wavelength as the MALS detector, for maximum accuracy in MALS calculations</li><li>Temperature range 4 °C to 65 °C for proteins and polymers that require a range of temperatures</li><li>High-resolution, touch-screen front panel displays data graphs and instrument diagnostics, even with the host computer turned off</li></ul><h5>Unparalleled features</h5><ul><li>Considering the extremely small peak volumes of UHPLC, band broadening in the microOptilab is kept to the absolute minimum, at less than 4 µL, as compared to 20 - 40 µL or more for standard RI detectors. As a result, the superior resolution from UHPLC columns and instruments will not be compromised with the microOptilab RI detector.</li><li>Even though the microOptilab offers a dRI measurement range orders of magnitude beyond that of other UHPLC dRI detectors, unlike other detectors there are no range or gain settings in the microOptilab. The full range of instrument detection is always present, and the full sensitivity exists over the entire range. The small flow cell and proprietary temperature regulation enable stable RI baselines and signals which further enhance the detector's sensitivity.</li><li>Unlike all other dRI detectors, the microOptilab also measures absolute refractive index in order to determine solvent RI, necessary in light scattering calculations and useful in assessing the accuracy of mixing of solvent gradients.</li><li>The microOptilab can be operated below ambient temperatures as easily as above ambient. No hassle temperature control can be programmed down to 4 °C or as high as 65 °C.</li><li>The microOptilab is compatible with all UHPLC systems.</li></ul><h4>Specifications</h4><h5>Measurement Capabilities – Differential Refractive Index</h5><ul><li>Differential RI Measurement Range: –0.0047 to +0.0047 RIU</li><li>Peak-to-Peak Noise: ±1.5 × 10⁻⁹ RIU (Measured per ASTM-E1303-95 at 25 °C with a 0.5 sec collection interval)</li><li>Response Time: User-selectable from 0.04 to 5 seconds</li><li>Absolute Refractive Index Range: 1.2 to 1.8 RIU</li><li>Absolute RI Sensitivity: ±0.002 RIU</li></ul>Fluidics and Flow Properties<ul><li>Band Broadening: &lt;4 µL (at 0.3 mL/min)</li><li>Fill Volume (Inlet Tubing + Flow Cell): 13.7 µL</li><li>Outlet Tubing Volume (0.030” ID): 228 µL</li></ul>Pressure Ratings<ul><li>Inlet Back Pressure (0.5 mL/min H₂O @ 20 °C): &lt;320 psi (22 bar)</li><li>Maximum Permissible Outlet Pressure: 30 psi (2 bar)</li></ul>Temperature Control<ul><li>Control Range: 4 °C to 65 °C</li><li>Stability: ±0.005 °C for precise thermal regulation</li></ul>Optical Source and Safety<ul><li>Light Source: 660 nm wavelength</li><li>Safety Sensors: Integrated vapor and liquid leak detection</li></ul>Electronics and Connectivity<ul><li>Digital Output Rate: 25 Hz</li><li>Analog Output: ±10 V or ±1 V, 18-bit resolution</li><li>Computer Interface: Ethernet for seamless data connectivity</li></ul>Auxiliary I/O Ports<ul><li>Inputs:<ul><li>Analog In (16-bit, ±10 V A/D)</li><li>Zero dRI</li><li>Purge</li><li>Recycle In</li><li>Autoinject In</li><li>Alarm In</li></ul></li><li>Outputs:<ul><li>Autoinject Retransmit</li><li>Alarm Out</li><li>Recycle Out (Orbit™ valve switching)</li></ul></li></ul>User Interface<ul><li>Display: 7” (178 mm) high-resolution multi-touch screen for viewing live signals, diagnostics, and configuring instrument settings</li></ul>Instrument Dimensions<ul><li>Size: 58 cm (L) × 36 cm (W) × 18 cm (H)</li></ul><h4>Other Refractive Index Detectors</h4><ul><li><a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/products/1268">Optilab™</a> - A unique on-line differential refractometer for measuring concentration of any macromolecule, regardless of chromophores. Temperature controlled from 4 °C to 65 °C. The high-concentration option accommodates protein concentration up to 180 mg/mL.</li></ul>

ECOM LED20M FIX Dual UV Detector with display

The LED20M FIX Dual UV Detector with a display uses Light Emitting Diodes (LED) as the light source. It is used for flash chromatography.

<div style="display:block;" aria-labelledby="tab_item-0">The LED20M FIX Dual UV Detector with a display uses Light Emitting Diodes (LED) as the light source. It is used for flash chromatography.<h3>Why choose LED20M FIX detectors for your application</h3><ul><li>The LED detectors allow easy and convenient detection in the UV-VIS range. They are manufactured in single or dual channel models with fixed wavelength setup.</li><li>The detectors are widely used in chromatography techniques using gravity glass columns for purification and isolation of organic small molecules and other compounds of interest. Long-lasting and reliable light source is provided by LED.</li><li>The optical flow cell is easily accessible and replaceable, this feature allows flexible choice of detection sensitivity if required. Low cell heating due to low power dissipation of LEDs makes this detector ideal for applications where analytes may be susceptible to degradation due to elevated temperatures. Absorbance information is displayed in detail on the integrated OLED display in numerical and graphical formats.</li><li>The units have convenient USB-C connectivity and power. When the detector is connected to a PC, the recorded data can be stored and processed using the ECOMAC software. ECOMAC basic software license is available with the price of the product without any additional fees. All these features and capabilities allow LED20M FIX to be an affordable, cost-effective, reliable and robust choice of an UV-VIS detector.</li></ul><h4>Wavelengths</h4><ul><li>The dual UV detector version measures at two fixed wavelengths, which are adjusted in production and given by the built-in LEDs.</li><li>By default, diodes with wavelengths of 255 and 280 nm are used. Optionally, the customer can select a wavelength in the range of 255–⁠⁠⁠⁠⁠⁠600 nm in steps of 5 nm.</li></ul><h4>Control</h4><ul><li>The detector can work without any additional SW support, but it is also possible to interconnect it via USB-C cable to a PC with ECOMAC SW, to get a data set for later use or to get reports for unit diagnostics.</li><li>The unit has an innovative PCB board that powers the LEDs, controls the unit, and has many new features, including higher sampling speed.</li></ul><h4>Key features and benefits</h4><ul><li>OLED display</li><li>Numerical and graphical view mode</li><li>Long lifetime of the LED light source</li><li>Low cell heating due to low power losses of the LEDs</li><li>USB C powering and communication port</li><li>ECOMAC Basic software for free</li><li>Autozero function</li><li>Easy flow cell replacement</li><li>Small dimensions</li><li>ECOMAC SW - &nbsp;sophisticated software for data acquisition and for diagnostics</li></ul></div>

ECOM LED20M FIX Single UV Detector with display

The LED20M FIX Single UV Detector with a display uses Light Emitting Diodes (LED) as the light source. It is used for flash chromatography.

<div style="display:block;" aria-labelledby="tab_item-0">The LED20M FIX Single UV Detector with a display uses Light Emitting Diodes (LED) as the light source. It is used for flash chromatography.<h3>Why choose LED20M FIX detectors for your application</h3><ul><li>The LED detectors allow easy and convenient detection in the UV-VIS range. They are manufactured in single or dual channel models with fixed wavelength setup.</li><li>The detectors are widely used in chromatography techniques using gravity glass columns for purification and isolation of organic small molecules and other compounds of interest. Long-lasting and reliable light source is provided by LED.</li><li>The optical flow cell is easily accessible and replaceable, this feature allows flexible choice of detection sensitivity if required. Low cell heating due to low power dissipation of LEDs makes this detector ideal for applications where analytes may be susceptible to degradation due to elevated temperatures. Absorbance information is displayed in detail on the integrated OLED display in numerical and graphical formats.</li><li>The units have convenient USB-C connectivity and power. When the detector is connected to a PC, the recorded data can be stored and processed using the ECOMAC software. ECOMAC basic software license is available with the price of the product without any additional fees. All these features and capabilities allow LED20M FIX to be an affordable, cost-effective, reliable and robust choice of an UV-VIS detector.</li></ul><h3>Wavelengths</h3><ul><li>The single UV detector version measures at 1 fixed wavelength, which is adjusted in production and given by the built-in LEDs.</li><li>By default, diodes with wavelengths of 255 or 280 nm are used. Optionally, customers can select a wavelength within the range of 255–⁠⁠⁠⁠⁠⁠600 nm in steps of 5 nm.</li></ul><h3>Control</h3><ul><li>The detector can work without any additional SW support, but it is also possible to interconnect it via USB-C cable to a PC with ECOMAC SW, to get a data set for later use or to get reports for unit diagnostics.</li><li>The unit has an innovative PCB board that powers the LEDs, controls the unit, and has many new features, including higher sampling speed.</li></ul><h3>Key features and benefits</h3><ul><li>Practical OLED display</li><li>Numerical and graphical view mode</li><li>Long lifetime of the LED light source</li><li>Low cell heating due to low power losses of the LEDs</li><li>USB -C powering and communication port</li><li>Autozero function</li><li>Easy flow cell replacement</li><li>Small dimensions</li><li>ECOMAC SW - &nbsp;sophisticated software for data acquisition and for diagnostics</li></ul>Note: A preparative cell PFC 200 Basic is supplied with the unit. Other flow cells are available at request.</div>

ECOM LED20G Dual UV Detector

LED20G Dual UV detector for preparative and flash chromatography. ECOM's unique patented technology radically improves the detectors´ features. LEDs are used as the source of light.

<div style="display:block;" aria-labelledby="tab_item-0">LED20G Dual UV LED detector for preparative and flash liquid chromatography. LEDs (Light Emitting Diodes) are used as the light source. A unique and patented ECOM technology radically improves the detector characteristics.&nbsp;The LEDs themselves typically have a peak wavelength with a tolerance of +/- 5 nm and a spectral half-width of 10-20 nm, which is unacceptable for some applications, as e.g. for FPLC. This ECOM solution, however, allows the spectral width to be narrowed and, thanks to our patented technology, the exact wavelength to be selected from the LED spectrum.This version of the dual UV detector measures at two fixed wavelengths given by the installed LEDs.The default wavelengths are 254 and 280 nm. Optionally, the customer can select a wavelength in the range 254-600 nm in 1 nm steps.The unit is controlled by an innovative printed circuit board that powers the LEDs, controls the unit, and has many new features including higher sampling speed.<h3>Key features and benefits</h3><ul><li>Sharp, high, and symmetrical peaks thanks to the ECOM patented technology</li><li>Excellent linearity</li><li>Excellent price-performance ratio</li><li>Compact device dimensions</li><li>Low cell heating due to LED low power supply (0.7 W) allows use for peptide analysis or FPLC</li><li>Easy flow cell replacement</li><li>Higher sampling speed</li><li>Sophisticated diagnostic software</li><li>Diodes with wavelengths of 254 and 280 nm and a PFC cell with an optical path length of 2 mm are used as standard.</li><li>Other cells and wavelengths on request.</li></ul><h3>Parameters</h3><ul><li>Number of channels (Signals): Two fix wavelength</li><li>Typical spectral half-width&nbsp;: 4</li><li>Light source: LEDs</li><li>Noise level at test cell (254 nm, TC 0.75 s): ± 10x10-6 AU</li><li>Drift at test cell (254 nm after 1 h): 1x10-3 AU/hr</li><li>Communication: Remote digital I/O</li><li>Power supply: 12-24 V DC</li><li>Power input: 2 W</li><li>Materials in contact with mobile phase: FEP; fused silica, stainless steel (cell)</li><li>Communication interface: USB-C</li><li>Optical cables: no</li><li>Dimensions W x H x D: 193 x 70 x 40 mm (7.60 x 2.76 x 1.57 in)</li><li>Weight: 0.66 kg (1.46 Ibs)</li></ul></div>

ECOM TOY18DAD 600 H Four Channel UV/PDA Detector

Four Channel version of our detector measures at four wavelengths simultaneously with possibility to see current scan and with built-in lamp working hours counter.

<div style="display:block;" aria-labelledby="tab_item-0">TOY18DAD 600 H Four Channel UV Detector is a standalone UV-VIS detector suited for preparative and flash chromatography with diode array (DAD) in range 200 – 600 nm.Four&nbsp;Channel&nbsp;version measures at&nbsp;four wavelengths simultaneously&nbsp;with possibility to see&nbsp;current scan.More wavelengths or scan are used in liquid chromatography to verify purity of analyzed samples or in situations when some peaks absorb on different wavelengths.<h4>Most important features of TOY18DAD H detectors:</h4><ul><li>built-in lamp working hours counter</li><li>easy replacement of flow cell</li><li>measuring at more wavelengths simultaneously</li><li>sophisticated diagnostic software</li></ul><h4>Parameters:</h4><ul><li>Number of channels (Signals): 4</li><li>Scan: Informative 200 – 600 nm</li><li>Number of CCD elements: 256</li><li>Communication: RS232, Ethernet (LAN), USB</li><li>Accuracy of adjustment: ± 1 nm</li><li>Typical spectral half-width: 10 nm</li><li>Reproducibility: ± 0.5 nm</li><li>Noise (Test cell, 254nm, TC 2s, 10 Hz): ± 5 x 10-5 AU</li><li>Drift (Test cell, 254nm): 1 x 10-3 AU/hr</li><li>Time constant: Time constant (T63) 0.5 s, 0.75 s, 1.0 s, 2.0 s, 4.0 s, 8.0 s, 16.0 s, 0.2 s, 0.1</li><li>Communication: RS232,Ethernet (LAN),USB</li><li>Power supply: 100-240V 50/60Hz</li><li>Power input: 100 VA (120 VA for upcoming version)</li><li>Materials in contact with mobile phase: FEP; fused silica, stainless steel, PEEK, KEL-F</li><li>Optical cables: no</li><li>Dimensions W x H x D: 280 x 135 x 463 mm (11.02 x 5.32 x 18.23 in)</li><li>Weight: 7.5 kg (16.53 lb)</li></ul></div>

Products from category HPLC detectors - page 2

Related content

Affinity, Gel Filtration, Size Exclusion Chromatography: Repurposing Kinase Inhibitors for Malaria

Research from IOCB Prague reveals a previously unknown mechanism of genetic transcription

Food Adulteration and How It Is Revealed by Analytical Chemistry