<p><strong>An international team led by researchers at CEITEC Masaryk University has, for the very first time, discovered that the tick-borne encephalitis virus is not as perfectly arranged as previously thought. Its surface contains areas where part of the protein coat is missing, leaving the viral membrane exposed. This incompleteness revealed that the rearrangement of surface proteins during viral maturation does not start simultaneously across the particle, but begins at a single point and then gradually spreads over the rest of the viral coat. The finding also suggests that the same principles apply to the entire group of flaviviruses, including dengue, Zika, and yellow fever viruses.</strong></p><p>Using <strong>cryo-electron microscopy and electron tomography</strong>, the researchers observed thousands of tick-borne encephalitis virus particles and found that many deviate significantly from the idealized textbook model. <i>“Flaviviruses are often depicted in textbooks as perfectly symmetrical particles. Our data, however, show that many immature and mature viral particles have <strong>exposed regions</strong> where part of the protein coat is missing. These imperfections helped us better understand how these viruses assemble and mature,”</i> says <a target="_blank" rel="noopener noreferrer" href="https://www.ceitec.eu/tibor-fuezik-ph-d/u89386?page=publication"><strong>Tibor Füzik</strong></a><strong> from CEITEC Masaryk University (MUNI), lead investigator of the study</strong>. The arrangement of surface proteins at the edges of these exposed areas indicates that the coat of immature particles assembles step by step from individual proteins. New data further suggest that the rearrangement into a mature structure also occurs gradually, starting from a randomly formed center and expanding by adding individual protein units along its edges.</p><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/CEITEC_Virus_klistove_encefalitidy_vedce_prekvapil_objevili_lysiny_v_jeho_obalu_2_4bdbba825f.jpg" alt="CEITEC/Plevka Lab: Tick-borne Encephalitis Virus Surprises Scientists: “Gaps” Discovered in Its Protein Coat: Immature (left) and mature (right) envelopes of tick-borne encephalitis virus particles contain disordered regions that expose the viral membrane." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_CEITEC_Virus_klistove_encefalitidy_vedce_prekvapil_objevili_lysiny_v_jeho_obalu_2_4bdbba825f.jpg 245w," sizes="100vw" width="1600" height="834"></p><p>Some parts of the viral surface proteins are normally hidden because they fit tightly together, making them difficult for antibodies to access. When part of the virus is not fully covered, these normally hidden protein regions are exposed, allowing antibodies – molecules used by the immune system to recognize and neutralize viruses – to attach. The discovery thus helps explain why some antibodies can effectively neutralize the virus, even though their target sites were previously thought to be inaccessible.</p><p>According to the study’s authors, this principle may not be limited to the tick-borne encephalitis virus. <i>“We believe that similar rules of assembly and maturation may also apply to other flaviviruses, such as dengue, Zika, or yellow fever viruses, which cause millions of infections worldwide each year,”</i> adds<strong> </strong><a target="_blank" rel="noopener noreferrer" href="https://www.ceitec.eu/pavel-plevka-ph-d/u26854"><strong>Pavel Plevka</strong></a><strong>, head of the research group at CEITEC MUNI</strong>.</p><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/CEITEC_Virus_klistove_encefalitidy_vedce_prekvapil_objevili_lysiny_v_jeho_obalu_06e750b1c0.jpg" alt="CEITEC/Plevka Lab: Tick-borne Encephalitis Virus Surprises Scientists: “Gaps” Discovered in Its Protein Coat: Tomographic analysis of tick-borne encephalitis virus particles shows irregularly shaped viral particles." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_CEITEC_Virus_klistove_encefalitidy_vedce_prekvapil_objevili_lysiny_v_jeho_obalu_06e750b1c0.jpg 235w," sizes="100vw" width="1600" height="1067"></p><p>Thanks to these new insights into<strong> how flaviviruses assemble and mature into their infectious form</strong>, scientists can better understand how the immune system recognizes viral particles and use this knowledge to inform the future development of antivirals or vaccines.</p><p>The research was conducted in collaboration with scientific teams from the <strong>University of Helsinki </strong>and the <strong>Faculty of Science at Masaryk University</strong>.</p><p>The study was published in <a target="_blank" rel="noopener noreferrer" href="https://www.science.org/doi/10.1126/sciadv.aee4765"><strong>Science Advances</strong></a>.</p><blockquote><h4><strong>The original article</strong></h4><h4><a target="_blank" rel="noopener noreferrer" href="https://www.science.org/doi/10.1126/sciadv.aee4765"><strong>Structures of asymmetric particles of tick-borne encephalitis virus provide insight into flavivirus assembly and maturation</strong></a></h4><p>Tibor Füzik, Maria Anastasina, Peter Pajtinka, Ausra Domanska, Lauri L. A. Pulkkinen, Lenka Smerdová, Lucie Nepovímová, Petra Formanová-Pokorná, Petra Straková, Jiří Nováček, Daniel Růžek, Robert Vácha, Sarah J. Butcher, Pavel Plevka</p><p><i><strong>Sci. Adv., 2026, 12, eaee4765</strong></i></p><p><a target="_blank" rel="noopener noreferrer" href="https://doi.org/10.1126/sciadv.aee4765"><span>https://doi.org/10.1126/sciadv.aee4765</span></a></p><h6>licensed under <a target="_blank" rel="noopener noreferrer" href="https://creativecommons.org/licenses/by/4.0/"><strong>CC-BY 4.0</strong></a></h6></blockquote><h4><strong>Abstract</strong></h4><p>Flaviviruses are globally distributed human pathogens. However, the mechanisms underlying flavivirus assembly and maturation remain poorly understood. Here, we show that many particles of tick-borne encephalitis virus (TBEV) are asymmetric and lack subsets of surface heterodimers. Immature particles of TBEV contain incomplete spikes, providing evidence that their coats assemble directly from heterodimers of premembrane (prM) and envelope (E) proteins. Exposure of TBEV particles to acidic pH in the Golgi complex promotes maturation. The spikes and herringbone regions in TBEV maturation intermediates are oriented randomly rather than conforming to a common icosahedral symmetry. Consequently, the mature herringbone lattice forms around a randomly oriented nucleation center, expanding by addition of membrane-envelope heterodimers as the spikes disassemble and prMs are cleaved. The observed incompleteness of the protein coats explains, as an alternative to particle breathing, how flaviviruses can be neutralized by antibodies that bind to regions of E proteins normally inaccessible in the spiky or herringbone structures.</p>

 Tick-borne Encephalitis Virus Surprises Scientists: “Gaps” Discovered in Its Protein Coat 

<p><strong>From environmental mandates to pharmaceutical breakthroughs, SPE has become the cornerstone of modern sample preparation.</strong></p><p>Walk into any modern analytical laboratory — pharmaceutical QC, environmental monitoring, food safety, forensics — and you will almost certainly find <a target="_blank" rel="noopener noreferrer" href="https://www.organomation.com/what-is-solid-phase-extraction-spe"><strong>solid phase extraction (SPE) </strong></a>at the heart of the sample preparation workflow. That is not a coincidence. Over the past decade, SPE has grown from a specialized cleanup technique into the dominant extraction method in laboratory science, and the forces accelerating its adoption are only intensifying.</p><p>At Organomation, we work alongside scientists who depend on reliable sample preparation every day. In this article, we explore why SPE has earned its leading position, where the market is heading, and what emerging application areas are putting new demands on SPE technology.</p><p>Responsible for roughly half of all lab extraction, SPE is the primary<a target="_blank" rel="noopener noreferrer" href="https://www.organomation.com/solvent-extraction-techniques"><strong> solvent extraction technique</strong></a> being adopted by laboratories in 2026.</p><h3><strong>The Fundamentals: What Makes SPE So Versatile?</strong></h3><p>Solid phase extraction is a preparative liquid technique that uses a solid stationary phase — typically silica, polymeric resins, or carbon-based sorbents packed into cartridges or columns — to selectively trap analytes of interest from complex sample matrices. Once retained on the sorbent, interferences can be washed away and the target compounds eluted in a concentrated, clean fraction ready for downstream analysis.</p><p>What sets SPE apart from competing techniques is its extraordinary versatility. The same fundamental workflow can be adapted for hydrophobic small molecules, polar pesticides, charged biomolecules, metal ions, and persistent environmental contaminants like PFAS — all by swapping the sorbent chemistry. This flexibility, combined with well-established protocols and vendor support across a mature market, means laboratories can standardize on SPE as a platform and customize it per application.</p><h3><strong>The LC-MS/MS Connection: Protecting Your Most Valuable Instrument</strong></h3><p>No single factor has done more to cement SPE's importance than the explosive growth of liquid chromatography-tandem mass spectrometry (<a target="_blank" rel="noopener noreferrer" href="https://www.organomation.com/preparing-samples-for-lc-ms/ms-analysis"><strong>LC-MS/MS</strong></a>) in pharmaceutical, clinical, and environmental laboratories. Tandem mass spectrometers are extraordinarily sensitive instruments — and extraordinarily sensitive to contamination.</p><p>Matrix components such as phospholipids, salts, proteins, and co-extracted organic matter can suppress ionization, foul ion sources, and produce false positives that invalidate entire analytical runs. SPE addresses this problem directly by leveraging electrostatic, hydrophobic, and hydrogen bonding interactions to purify and pre-concentrate analytes before they ever reach the instrument. The result is cleaner chromatography, better signal-to-noise ratios, and longer instrument lifespans — all of which translate into real cost savings and better data quality.</p><p>For laboratories running high-throughput quantitative assays — therapeutic drug monitoring, environmental contaminant panels, metabolomics studies — SPE is not optional. It is the critical gatekeeper that makes the downstream analysis possible.</p><h3><strong>The Automation Wave: Doing More with Less</strong></h3><p>If the LC-MS/MS partnership explains why SPE became essential, automation explains why it is growing. Laboratory staffing pressures, increasing sample volumes, and zero-tolerance regulatory environments are forcing labs to rethink manual workflows. SPE, historically a hands-on technique, has been at the forefront of this transformation.</p><h4><strong>Online SPE Systems</strong></h4><p>Modern online SPE platforms couple extraction directly with HPLC, eliminating the offline sample preparation step entirely. In these configurations, the SPE column is integrated into the chromatographic flow path, and sample loading, washing, and elution occur automatically within the analytical run cycle. This approach dramatically reduces analyst time per sample, minimizes the risk of sample loss or contamination during transfer, and enables faster turnaround in high-throughput environments.</p><h4><strong>Multi-Channel Automated Systems</strong></h4><p>At the high-throughput end of the market, automated multi-channel SPE systems allow laboratories to process eight samples simultaneously — each channel independently controlled — with positive-pressure delivery that applies consistent, programmable flow rates across every sample. The reproducibility gains are significant: automated systems eliminate the variability introduced by individual analyst technique, which is particularly important in regulated environments where method validation requirements are strict.</p><p>Organomation partner PromoChrom Technologies offers two compelling examples of this category. The <a target="_blank" rel="noopener noreferrer" href="https://blog.organomation.com/blog/promochrom-extraction-and-organomation-evaporation-for-epa-pfas-methods"><strong>SPE-03</strong></a>, PromoChrom's established 8-channel workhorse, uses a patented flow-path-integration valve to control all eight channels through a single compact module — enabling parallel processing, drying the cartridge with nitrogen, solvent mixing, and automated bottle rinsing without the bulk of traditional robotic liquid handlers. The newer Presto system pushes throughput further still, with built-in continuous pumps capable of loading eight one-liter water samples in approximately 20 minutes, making it particularly well suited to large-volume environmental extractions. Both systems run fully unattended after setup, requiring only a few minutes of analyst involvement per batch.</p><h3><strong>The PFAS Mandate: A Regulatory Catalyst for SPE Innovation</strong></h3><p>Perhaps the most consequential near-term driver for SPE technology investment is the global regulatory focus on <a target="_blank" rel="noopener noreferrer" href="https://blog.organomation.com/blog/the-importance-of-detecting-per-and-polyfluoroalkyl-substances-pfas"><strong>per- and polyfluoroalkyl substances</strong></a>, better known as PFAS or "forever chemicals." These synthetic compounds, used in everything from firefighting foam to non-stick cookware, have proven extraordinarily persistent in the environment — and increasingly, regulators are demanding that laboratories detect them at trace levels in complex matrices.</p><h4><strong>The U.S. Regulatory Picture</strong></h4><p>In April 2024, the U.S. Environmental Protection Agency issued the first nationally enforceable<a target="_blank" rel="noopener noreferrer" href="https://www.faegredrinker.com/en/insights/publications/2025/1/year-in-review-2024-federal-pfas-roundup"><strong> drinking water standards for six PFAS compounds</strong></a>, establishing Maximum Contaminant Levels (MCLs) as low as<strong> 4 parts per trillion for PFOA and PFOS</strong>. The rule is expected to reduce PFAS exposure for approximately <strong>100 million Americans</strong>. Simultaneously, the EPA finalized new test methods — including Method 1633, which tests for 40 PFAS across wastewater, groundwater, soil, sediment, and fish tissue — that are already shaping laboratory practice nationwide.</p><p>While the EPA announced in<strong> May 2025</strong> that it intends to retain MCLs for PFOA and PFOS while reconsidering standards for four additional PFAS, the fundamental demand for sensitive PFAS testing infrastructure remains strong. Compliance deadlines for PFOA and PFOS have been extended to 2031, giving water utilities time to invest in testing and treatment — and sustaining demand for high-quality analytical methods throughout that period.</p><h4><strong>What PFAS Analysis Demands of SPE</strong></h4><p>PFAS analysis at parts-per-trillion concentrations in drinking water, wastewater, soil, and food matrices is among the most demanding applications in environmental chemistry. Interference from co-extractants is severe, and background contamination from PFAS in laboratory plasticware and solvents must be meticulously controlled. Vendors have responded by developing specialized SPE media engineered specifically for PFAS capture and cleanup — including stacked cartridge designs that layer multiple sorbent chemistries to handle the full range of PFAS compound classes in a single pass. These application-specific products represent a new tier of SPE innovation driven directly by regulatory pressure.</p><h3><strong>Why more labs will continue to adopt SPE</strong></h3><p>The global SPE market continues to expand, driven by intersecting forces across pharmaceutical, environmental, food safety, and forensic laboratories. Several application trends stand out as particularly important drivers of new SPE adoption and innovation.</p><h4><strong>Pharmaceutical and Biotech Expansion</strong></h4><p>Pharmaceutical and biopharmaceutical QC represent the most mature and demanding SPE application areas. Complex biological matrices — plasma, urine, tissue homogenates — require aggressive cleanup to remove phospholipids, proteins, and endogenous interferences before LC-MS/MS quantitation. Tightening regulatory standards for drug safety and the rapid growth of biotherapeutics, including monoclonal antibodies and cell and gene therapy products, are pushing sorbent development into novel territory with hybrid mixed-mode chemistries and size-selective media gaining ground.</p><h4><strong>Environmental Monitoring Intensification</strong></h4><p>Environmental monitoring is an area of intense growth for SPE. Beyond PFAS, labs are contending with expanding panels of emerging contaminants including microplastics, endocrine disruptors, and pharmaceutical residues in surface and groundwater. Each new contaminant class brings its own matrix challenges — pH sensitivities, competing co-extractants, low log P values — that drive the development of new sorbent chemistries and method-specific SPE products.</p><h4><strong>Food Safety and Forensics</strong></h4><p>Food safety laboratories are under continuous pressure to detect lower levels of mycotoxins, veterinary drug residues, pesticides, and processing contaminants in increasingly complex matrices — grain products, dairy, beverages, seafood. Forensic toxicology, meanwhile, faces growing caseloads driven by opioid testing, workplace drug screening, and poisoning investigations, all of which rely on SPE for the sample cleanup that makes definitive LC-MS/MS confirmation possible.</p><h4><strong>Greener SPE</strong></h4><p>An emerging trend worth watching is the development of more environmentally sustainable SPE consumables. Biodegradable SPE columns designed to reduce plastic waste in single-use applications — responding to mounting pressure from green chemistry initiatives and laboratory sustainability programs. As laboratories face increasing scrutiny of their environmental footprint, sustainable alternatives to conventional plastic cartridges may gain significant traction.</p><h3><strong>How Organomation Supports Your SPE Workflow</strong></h3><p>Organomation has built its reputation on evaporation and concentration instruments that work directly downstream of SPE — precisely the sample preparation workflow where SPE's pre-concentration efficiency is most valuable. After analytes are eluted from SPE cartridges, they typically need to be reconstituted in a small volume of compatible solvent before injection. That is where Organomation's nitrogen evaporators, the <a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/products/2408"><strong>N-EVAP </strong></a>and <a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/products/2417"><strong>MULTIVAP</strong></a> series deliver consistent, reproducible results at the speed modern laboratories demand.</p><p>Whether your laboratory is developing PFAS methods to meet new EPA requirements, processing high-volume pharmaceutical QC samples, or building out an environmental monitoring program, our team understands the complete sample preparation workflow — and is available to help you select the right evaporation and concentration solution for your specific application.&nbsp;</p>

Why Solid Phase Extraction Is Having Its Moment

<p><strong>Our Library never stops expanding. What are the most recent contributions to LabRulezLCMS Library in the week of 10th August 2026? Check out new documents from the field of liquid phase, especially HPLC and LC/MS techniques!</strong></p><blockquote><p><span style="font-size:20px;">👉 </span><a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/library"><span style="font-size:20px;"><strong>SEARCH THE LARGEST REPOSITORY OF DOCUMENTS ABOUT LCMS AND RELATED TECHNIQUES</strong></span></a></p></blockquote><blockquote><p><strong>👉 Need info about different analytical techniques?</strong> Peek into <a target="_blank" rel="noopener noreferrer" href="https://gcms.labrulez.com/library"><strong>LabRulezGCMS</strong></a> or <a target="_blank" rel="noopener noreferrer" href="https://icpms.labrulez.com/library"><strong>LabRulezICPMS</strong></a> libraries.</p></blockquote><p>This week we bring you posters by <strong>Agilent Technologies / ASMS, Thermo Fisher Scientific / ASMS </strong>and<strong> Waters Corporation / ASMS </strong>and application notes by<strong> Metrohm and Shimadzu!</strong></p><h3><strong>1. Agilent Technologies / ASMS: Impurity Monitoring of GLP-1 Therapeutic Peptide RetatrutideUsing a 2D-LC/TOF System</strong></h3><ul><li>Poster</li><li><a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/labrulez-bucket-strapi-h3hsga3/glp_1_impurity_monitoring_2d_lc_tof_asms_2026_mp_388_agilent_eadce3f244.pdf"><strong>Full PDF for download</strong></a></li></ul><p>Retatrutide is a next-generation triple agonist (GLP1/GIP/glucagon) and an emerging candidate among multivalent GLP1–based therapeutics. Its structural complexity and diverse impurity profile challenge conventional one-dimensional LC methods, which often lack sufficient resolving power to separate closely related impurities, sequence variants, and synthetic byproducts.&nbsp;</p><p>In this study, we demonstrate an Agilent multiple heart-cutting 2D-LC platform that enables rapid online solvent exchange and enhanced chromatographic selectivity, allowing targeted isolation of impurity peaks from a TFA-based first-dimension separation. When coupled with the Agilent 6230C Accurate Mass TOF LC/MS system, the workflow delivers fast, sensitive, and accurate mass profiling under MS-friendly second-dimension conditions, providing a robust solution for comprehensive impurity characterization of GLP-1 peptides such as Retatrutide.</p><h4><strong>Experimental</strong></h4><h5><strong>Instrumentation&nbsp;</strong></h5><ul><li><a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/library?instrumentationItems=39&amp;mainauthorItems=10&amp;search=1290+infinity+III+2D-LC"><strong>Agilent 1290 Infinity III 2D-LC system</strong></a><strong> comprised the following modules:&nbsp;</strong><ul><li>1290 Infinity III Bio Multisampler with Thermostat (G7137A)&nbsp;</li><li>1290 Infinity III Multicolumn Thermostat (G7116B)&nbsp;</li><li>1290 Infinity III Diode Array Detector (G7117B) with Max-Light cartridge cell (G7117-60020)&nbsp;</li><li>1260 Infinity III Bio Flexible Pump (G7131C)&nbsp;</li><li>1290 Infinity III Bio High-speed Pump (G7132A)&nbsp;</li><li>1290 Infinity Valve Drive (G1170A) with 2D-LC ASM Valve (G5643B)&nbsp;</li><li>Two 1290 Infinity III Valve Drives (G1170A) with multiple heart-cutting valves (G5642-64000) equipped with 40 µL Bio loops&nbsp;</li></ul></li><li><a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/library?mainauthorItems=10&amp;search=6230C"><strong>Agilent 6230C Accurate Mass TOF LC/MS System</strong></a></li></ul><h4><strong>Conclusions</strong></h4><p>Agilent 2D-LC with multiple heart-cutting effectively bridges TFA-driven chromatographic performance with FA-compatible MS detection, overcoming ion suppression limitations.&nbsp;</p><p>The integrated 2D-LC/Accurate Mass TOF workflow delivers confident impurity identification and robust characterization, supporting peptide analysis in both QA/QC and development environments.</p><h3><strong>2. Metrohm: Vinylene carbonate water determination via coulometric Karl Fischer titration</strong></h3><p><strong>Water determination in vinylene carbonate by Karl Fischer coulometric titration made simple with alcohol-free reagents</strong></p><ul><li>Application note</li><li><a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/labrulez-bucket-strapi-h3hsga3/an_k_076_download_8658665940.pdf"><strong>Full PDF for download</strong></a></li></ul><p>Vinylene carbonate (vc) is commonly used as a battery electrolyte additive in lithium-ion batteries. Its popularity is due to the positive impact it has on anode cyclability and reduction of irreversible capacity. Small amounts of moisture in Li-ion batteries can significantly decrease performance and lifetime. Therefore, moisture levels in electrolyte solutions and their additives are controlled for use in manufacturing. Until recent years, accurate moisture quantification in vinylene carbonate samples was difficult due to side reactions. With the commercial development of alcohol-free coulometric Karl Fischer reagents, accurate determination is now much more accessible to end-users.</p><h4><strong>EXPERIMENTAL</strong></h4><p>This analysis was performed using an <a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/library?mainauthorItems=220&amp;search=852+titrando"><strong>852 Titrando</strong></a> (2.852.0060) instrument, equipped with the electrodes listed in Table 1.</p><h4><strong>CONCLUSION&nbsp;</strong></h4><p>During battery manufacturing, control of all moisture present is important to guarantee adequate performance in the finished product. More easily quantifying the moisture contribution from additives such as vinylene carbonate makes this a much simpler task. The introduction of alcohol-free Karl Fischer reagents accomplishes this effectively, allowing the generation of accurate and repeatable moisture data—even for a difficult sample matrix.</p><h3><strong>3. Shimadzu: Non-Targeted Analysis of Metabolites in Alcoholic Beverages Using LabSolutions Insight Profiler</strong></h3><ul><li>Application note</li><li><a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/labrulez-bucket-strapi-h3hsga3/an_01_01241_en_57b43edb57.pdf"><strong>Full PDF for download</strong></a></li></ul><h5><strong>User Benefits</strong></h5><ul><li>Using an LC-QTOFMS system with LabSolutions Insight Profiler non-targeted analysis software enables comprehensive comparative analysis of metabolites in food samples.&nbsp;</li><li>Principal component analysis and volcano plots can be used to detect characteristic features in each sample.&nbsp;</li><li>A variety of functions, including MS/MS spectral library searching, enable easy qualitative analysis of components.</li></ul><p>Metabolomics, a technique that analyzes metabolites in living organisms, is increasingly being applied in the food industry for product development and quality control. A particularly powerful approach is non-targeted metabolomics, which aims to analyze not only known metabolites but also unknown compounds without prior assumptions to reveal unexpected chemical differences between samples. Quadrupole time-of-flight mass spectrometry (QTOFMS), with its accurate-mass capability, is well suited for this purpose. However, the large volume of generated data creates challenges for feature identification and data comparison.&nbsp;</p><p>This article presents a case study in which LabSolutions Insight Profiler, a software platform for non-targeted analysis, was used to characterize compounds in beer and beer-like beverages. Peak detection, retention-time alignment, and library searching were performed across multiple datasets. Multivariate analysisrevealed compound profiles that likely reflect the differences in raw materials and manufacturing processes, demonstrating the utility of the approach for beverage characterization.</p><h4><strong>LabSolutions Insight Profiler&nbsp;</strong></h4><p><a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/library?mainauthorItems=1&amp;search=LabSolutions+Insight+Profiler"><strong>LabSolutions Insight Profiler </strong></a>is software for batch processing of LC-QTOFMS data that enables non-targeted analysis through an intuitive workflow. It readily supports easy spectral feature detection, alignment, statistical processing, filtering, list screening, MS/MS spectra-based library searching, principal component analysis (PCA), displaying volcano or box plots, and more. Fig. 1 shows the data analysis workflow.</p><h4><strong>Analysis Conditions&nbsp;</strong></h4><p><a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/library?mainauthorItems=1&amp;search=nexera+X3"><strong>Nexera X3 UHPLC</strong></a> and<strong> </strong><a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/library?mainauthorItems=1&amp;search=9030"><strong>LCMS-9030 systems</strong></a> were used as the analytical instruments. The LC method included in the LC/MS/MS Method Package for Primary Metabolites was used as the method. The data-dependent acquisition (DDA) mode was used to simultaneously acquire precursor m/z and MS/MS data. Table 2 shows the analysis conditions.</p><h4><strong>Conclusion&nbsp;</strong></h4><p>In this study, compounds in various beer and beer-like beverages were analyzed by non-targeted analysis using an LCMS-9030 quadrupole time-of-flight mass spectrometer system. Analyzing compounds characteristic of each sample with LabSolutions Insight Profiler software confirmed that the resulting profiles reflected differences in raw materials and manufacturing methods.&nbsp;</p><p>LabSolutions Insight Profiler enables efficient non-targeted analysis by processing LC-QTOFMS data with an intuitive workflow. This workflow allows for objective evaluation of the taste, quality, and nutritional value of food products. Furthermore, this method is applicable not only to the analysis of compounds in beverages but also to non-targeted analysis of other types of samples.</p><h3><strong>4. Thermo Fisher Scientific / ASMS: High throughput targeted proteomic workflows with Adaptive Retention Time on the </strong><a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/products/1959"><strong>Orbitrap Astral Zoom MS</strong></a></h3><ul><li>Poster</li><li><a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/labrulez-bucket-strapi-h3hsga3/po_004699_lsms_art_astral_zoom_proteomics_po004699_asms2026_na_en_78767efdc9.pdf"><strong>Full PDF for download</strong></a></li></ul><p>Advances in MS instrumentation have accelerated the identification of biomarkers, but validating and translating these discoveries into assays for clinical use remains challenging and typically requires robust and accurate targeted approaches. While these often rely on predetermined reference target retention time (RT) windows, analytical column degradation or replacement, aging solvents, sample matrix effects, etc. can cause peptide RT shifts between injections and across longer-term analyses. To accommodate this, targeted MS experiments generally schedule wide target RT windows (&gt;1 min) or rely on heavy labeled peptides to trigger acquisition of endogenous peptides. Both greatly limit the number of quantifiable targets per assay. To overcome this, we utilize adaptive RT (aRT) alignment for live correction of RTs together with the speed and sensitivity of the <a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/products/1959"><strong>Orbitrap Astral Zoom MS</strong></a>. This allows reproducible tracking of &gt;2000 targets with narrow RT windows, high throughputs, such as 300 samples per day (SPD), and hybrid workflows combining targeting, of up to 1000 targets, and discovery.</p><h4><strong>Materials and methods&nbsp;</strong></h4><h5><strong>LC-MS Experiments&nbsp;</strong></h5><p>Experiments were performed using Thermo Scientific Pierce HeLa protein digest resuspended in 0.1% TFA, or individual or pooled human plasma samples. Peptides were separated using a 15 cm Thermo Scientific PepMap analytical columns (150 µm inner diameter) and the <a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/products/398"><strong>Thermo Scientific Vanquish Neo UHPLC system</strong></a> at throughputs of 60 or 300 SPD. The liquid chromatography (LC) system was interfaced with the <a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/products/1959"><strong>Orbitrap Astral Zoom MS.</strong></a> Reference runs using MS1 and/or data-independent-acquisition (DIA) scans were acquired to obtain reference aRT spectra, and to determine target RTs. For aRT alignment, methods consisting of aRT MS1 and/or DIA scans, as well as targeted MS2 scans with RT correction were optimized. For hybrid DIA workflows, we used aRT MS1 scans for reference acquisition and alignment, combined with narrow window DIA and a targeted MS2 experiment. Observed RT shifts were caused by natural column degradation, sample matrix effects or when indicated induced by variation in gradient flow rate and buffer composition.&nbsp;</p><h5><strong>Data analysis&nbsp;</strong></h5><p>Reference DIA data was analyzed using Spectronaut. Targets lists were then generated from the search output by selecting varying number of targets evenly distributed across the RT dimension. Target RTs for smaller scale assays were manually validated and exported from Skyline, while target RTs for large scale assays were exported from Spectronaut. For alignment runs, DIA and PRM data were analyzed using Spectronaut and Skyline respectively.</p><h4><strong>Conclusions&nbsp;</strong></h4><ul><li>Adaptive RT alignment on Orbitrap Astral Zoom MS shows robustness against various sources of RT shifts and has high target recovery even for thousands of targets&nbsp;</li><li>aRT enables the use of target acquisition windows &lt;15 s for robust targeting at high throughput&nbsp;</li><li>aRT Hybrid DIA allows targeting of up to 1000 targets with minimal cost to DIA depth</li></ul><h3><strong>5. Waters Corporation / ASMS: BOOSTING SENSITIVITY IN TOP-DOWN PROTEIN STUDIES USING CYCLIC IMS-ENABLED WIDEBAND ENHANCEMENT</strong></h3><ul><li>Poster</li><li><a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/labrulez-bucket-strapi-h3hsga3/720009471_51f2e8b546.pdf"><strong>Full PDF for download</strong></a></li></ul><div><div><section><div><div><div><div><div><div><p>The poster evaluates <strong>Wideband Enhancement (WBE)</strong> as a way to improve sensitivity in top-down protein mass spectrometry. In top-down workflows, intact proteins are fragmented directly, but the signal is distributed across multiple charge states, isotope envelopes, and numerous product ions, which can limit MS/MS sensitivity. WBE addresses this problem by synchronizing ion packets leaving the ion mobility region with the time-of-flight pusher, thereby increasing the number of ions that reach the detector within a selected <i>m/z</i> range.</p><p>Experiments were performed using a <a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/products/2434"><strong>Waters Cyclic IMS P20 Mass Spectrometer</strong></a> equipped with an <strong>electron capture dissociation cell</strong>. Samples were introduced by direct infusion in positive electrospray ionization mode. Two proteins were studied under denaturing conditions: bovine ubiquitin, approximately 8.5 kDa, and bovine carbonic anhydrase II, approximately 29 kDa. Single-pass and bypass ion mobility measurements were carried out with and without WBE, using both <strong>collision-induced dissociation (CID)</strong> and <strong>electron capture dissociation (ECD)</strong>. Data were acquired in <a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/products/635"><strong>MassLynx</strong></a><strong> v4.2</strong> and processed using <strong>DriftScope</strong> and the <strong>waters_connect UNIFI peptide-mapping workflow</strong>.</p><p>For ubiquitin, WBE substantially increased the intensity of CID- and ECD-derived fragment ions. Improvements of up to tenfold were observed across the c- and z-ion series, while bypass ion mobility mode produced the largest overall sensitivity gain. The results indicate that WBE can enhance fragment detection while retaining the benefits of cyclic ion mobility separation and without sacrificing mass-spectral resolution.</p><p>The larger carbonic anhydrase II protein also showed marked improvements. CID fragment signals increased by up to tenfold for lower-<i>m/z</i> ions and about eightfold for higher-<i>m/z</i> fragments. Because ECD is generally less efficient than CID, the benefit of WBE was especially evident in ECD experiments: sequence coverage increased from <strong>54% without WBE to 70% with WBE</strong> in bypass mode. The authors conclude that combining WBE with complementary CID and ECD fragmentation on the Cyclic IMS P20 enables more sensitive and comprehensive top-down protein characterization.</p></div></div></div></div></div></div></section></div></div>

News from LabRulezLCMS Library - Week 33, 2026

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Waters/Wyatt DAWN™ Multi Angle Light Scattering (MALS) Detector

The world's most advanced light scattering instrument for absolute characterization of proteins, conjugates, macromolecules, and nanoparticles.

<h5><strong>Premier MALS</strong></h5><p>The DAWN instrument is the premier Multi-Angle static Light Scattering (MALS) detector for absolute characterization of the molar mass and size of macromolecules and nanoparticles in solution. It offers the highest sensitivity, the widest range of molecular weight, size, and concentration, and the largest selection of configurations and optional modules for enhanced capabilities.</p><h5><strong>Separation First</strong></h5><p>MALS is most often used in conjunction with a separation system such as Gel Permeation Chromatography (GPC-MALS), Size Exclusion Chromatography (SEC-MALS), or Field-Flow Fractionation (FFF-MALS) to determine distributions of mass, size, and composition, independent of column calibration by reference standards.</p><h5><strong>Supported by ASTRA ™ &nbsp;Software</strong></h5><p>ASTRA Software and its built-in analysis modules support the DAWN MALS instrument, providing the most versatile analysis available for online multi-angle and dynamic light scattering measurements.</p><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Waters_DAWN_MALS_HPLC_detektor_konfigurace_d5b83e46b2.jpg" alt="Waters DAWN™ Multi Angle Light Scattering (MALS) Instrument: ASTRA Software" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Waters_DAWN_MALS_HPLC_detektor_konfigurace_d5b83e46b2.jpg 245w," sizes="100vw" width="1500" height="807"></p><h4><strong>Product description</strong></h4><p>The DAWN and its companion <strong>Optilab ™ &nbsp;dRI detector</strong> are the established benchmarks for MALS analysis, cited in thousands of peer-reviewed publications. Multi-angle light scattering detection is indispensable for use with <strong>GPC</strong> and <strong>HPLC-SEC </strong>in order to obtain reliable molecular mass distributions and information on molecular conformation, branching ratio, fragments and aggregates.</p><p><strong>SEC-MALS</strong> with a <strong>DAWN</strong> and <strong>Optilab</strong> characterizes conformation and branching of synthetic and natural <strong>polymers larger than 10 nm</strong> in rms radius R g . The addition of a <strong>ViscoStar ™&nbsp;</strong> differential viscometer creates a GPC triple-detection system that can analyze molecular conformation and size below the R g &nbsp;limit of 10 nm and determine Mark-Houwink Sakurada coefficients.</p><p>The combination of a UV/Vis absorbance detector with a DAWN instrument and Optilab refractive index detector creates a UV-MALS-RI triple-detection system, for analysis of copolymers and protein conjugates (glycoproteins, PEGylated proteins, surfactant-bound membrane proteins, etc.). With the addition of a <strong>WyattQELS ™ &nbsp;DLS module</strong> for size determination, protein conformation may be analyzed.</p><ul><li><i>M<sub>w</sub></i> &nbsp;from 200 Da to 1000 MDa</li><li><strong>Sensitivity:</strong> 10 ng/mL 100 kDa polystyrene in THF, 0.4 µg/mL BSA (66.4 kDa) in PBS</li><li><i>R<sub>g</sub></i> &nbsp;from 10 to 500 nm, up to 1000 nm with shape-specific models</li><li>Ultra-stable, high-power laser</li></ul><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Waters_DAWN_MALS_HPLC_detektor_konfigurace_1_98811a1e87.jpg" alt="Waters DAWN™ Multi Angle Light Scattering (MALS) Instrument: Accessories" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Waters_DAWN_MALS_HPLC_detektor_konfigurace_1_98811a1e87.jpg 213w," sizes="100vw" width="1000" height="733"></p><p>The DAWN may also be used in batch (off-line) mode to characterize the weight-average molar mass, z-average size and second virial coefficient of unfractionated samples.</p><p>In conjunction with a <strong>Calypso ™ &nbsp;CG-MALS system</strong>, the DAWN characterizes macromolecular interactions, label-free and immobilization-free: affinity and absolute stoichiometry of self- and hetero-association of simple or complex molecular systems. The Calypso system also automates the determination of second virial coefficients, cross virial coefficients, average molar masses and sizes.</p><h5><strong>Ultrasonic Cleaning Included</strong></h5><p>Each DAWN instrument includes an embedded <strong>COMET ™ &nbsp;ultrasonic transducer</strong> that cleans the flow cell automatically by agitating the fluid and loosening particles from the glass surfaces.</p><h5><strong>Temperature-Controlled Models</strong></h5><ul><li>Ambient model</li><li>Ultra-High-Temperature (UHT) model, from ambient up to +210 °C</li><li>Standard Peltier heated/cooled (H/C) model, temperature control from -15 °C to +150 °C</li></ul><h4><strong>Specifications</strong></h4><h5><strong>Measurement Capabilities</strong></h5><ul><li><strong>Molar Mass Range:</strong> From 200 Da to 1 GDa, depending on dn/dc, sample concentration, and chromatography conditions.</li><li><strong>Molecular Size (Radius of Gyration, Rg):</strong> Approximately 10 nm to 500 nm, extendable to 1000 nm with shape-specific models.</li><li><strong>Hydrodynamic Radius (Rh):</strong><ul><li><strong>Flow mode:</strong> 0.5 nm to 300 nm (depending on configuration and flow rate).</li><li><strong>Batch mode:</strong> 0.5 nm to 1 µm (requires WyattQELS™ DLS module or DynaPro™ NanoStar™ with fiber optic connection).</li></ul></li><li><strong>Sensitivity:</strong> Detects as little as 10 ng of 100 kDa polystyrene in THF or 200 ng of BSA in PBS, using a standard 7.8 mm x 300 mm SEC column and no post-column filter.</li></ul><h5><strong>Fluidics and Compatibility</strong></h5><ul><li><strong>Mobile Phase:</strong> Compatible with both aqueous and organic solvents.</li><li><strong>Wetted Materials:</strong> 316 stainless steel, fused silica or F2 glass, and Kalrez.</li></ul><h5><strong>Optical System and Detectors</strong></h5><ul><li><strong>MALS Detectors:</strong> 18 photodiodes with high gain and wide dynamic range for multi-angle light scattering.</li><li><strong>Auxiliary Detectors:</strong><ul><li>Laser monitor for stabilization</li><li>Forward transmission monitor to improve data quality and correct for sample absorption</li></ul></li><li><strong>Dynamic Range:</strong> 3,300,000:1</li><li><strong>Optional DLS Detection:</strong><ul><li>Integrated WyattQELS™ module inside the DAWN</li><li>External fiber optic connection with DynaPro NanoStar or Mobius DLS-ELS instruments</li></ul></li></ul><h5><strong>Laser Specifications</strong></h5><ul><li><strong>Wavelength:</strong> Standard 658 nm ± 4 nm; optional 785 nm for fluorescent samples</li><li><strong>Power Control:</strong> Programmable from 10% to 100%</li></ul><h5><strong>Flow Cell Options</strong></h5><ul><li><strong>Fused Silica:</strong> Ideal for solvents with refractive index &lt; 1.50</li><li><strong>F2 Glass:</strong> Recommended for solvents with refractive index &gt; 1.50</li></ul><h5><strong>Temperature Control Options</strong></h5><ul><li><strong>Ambient operation</strong></li><li><strong>Heated/Cooled (HC):</strong> -15 °C to +150 °C</li><li><strong>Ultra-High Temperature (UHT):</strong> Ambient to +210 °C</li></ul><h5><strong>Electronics and Connectivity</strong></h5><ul><li><strong>Analog Inputs:</strong> 4 differential inputs, 24-bit resolution, range -10 V to +10 V</li><li><strong>Analog Outputs:</strong> 2 outputs from selectable measurement channels, -10 V to +10 V</li><li><strong>I/O Options:</strong> Alarm in/out, auto-inject control, and Orbit™ valve integration</li><li><strong>Computer Interface:</strong> Ethernet</li><li><strong>Display:</strong> 10.1” high-resolution capacitive touchscreen with multi-touch controls</li></ul><h5><strong>Instrument Dimensions</strong></h5><ul><li><strong>Size:</strong> 58 cm (L) x 36 cm (W) x 26 cm (H)</li></ul><h4><strong>Other MALS Instruments</strong></h4><ul><li><a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/products/1115"><strong>miniDAWN™</strong></a> &nbsp;- Second only to the DAWN in sensitivity. Incorporates detectors at 3 angles to determine molar masses from 200 Da to 10 MDa and radii from 10 – 50 nm. Ambient only.</li><li><a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/products/1116"><strong>microDAWN™</strong></a><strong>&nbsp;</strong> - The first MALS detector for UHPLC, with interdetector dispersion as low as 1.5 µL. Incorporates detectors at 3 angles to determine molar masses from 200 Da to 20 MDa and radii from 10 – 50 nm. Ambient only.</li><li><strong>ultraDAWN™</strong> &nbsp;- Measures multi-angle light scattering and reports the results – weight-average molecular weight <i>M<sub>w</sub></i> &nbsp;and rms radius <i>R<sub>g</sub></i> &nbsp;– in real time.</li></ul>

Waters/Wyatt miniDAWN™ Multi Angle Light Scattering (MALS) Detector

The Best in Basic Multi-Angle Light Scattering for Absolute Macromolecular Analysis.

<h4><strong>Overview</strong></h4><h5><strong>Molar Mass</strong></h5><p>The miniDAWN determines absolute molar mass and size to characterize proteins, peptides, small polymers and nanoparticles in solution. Coupled to size exclusion chromatography (SEC or GPC), it eliminates the uncertainty and hassle of column calibration, for true confidence in macromolecular characterization.</p><h5><strong>Superb Sensitivity</strong></h5><p>The miniDAWN not only offers superb sensitivity—it further enhances productivity thanks to greatly enhanced field serviceability and easy data analysis with <strong>One-Click </strong><i><strong>M<sub>w</sub></strong></i><strong>™</strong> .</p><h5><strong>Compatible with ASTRA™&nbsp;</strong></h5><p>The miniDAWN is accompanied by <strong>ASTRA,</strong> the most versatile software package available for analysis of online multi-angle and dynamic light scattering. ASTRA and the miniDAWN incorporate advanced ease-of-use features that help guarantee excellent measurements with a single click.</p><h4><strong>Product Description</strong></h4><h5><strong>Molar mass, absolutely</strong></h5><p>Wyatt's miniDAWN is most often used in conjunction with size exclusion chromatography (SEC-MALS) or field-flow fractionation (FFF-MALS) to determine distributions of molecular weight, size and composition. No need to make assumptions about molecular conformation or ideal column interactions.</p><h5><strong>Characterize:</strong></h5><ul><li>Proteins, small peptides and oligonucleotides</li><li>Synthetic and natural polymers</li><li>Nanoparticles, virus-like particles and vesicles</li></ul><h5><strong>Measurement Range:</strong></h5><ul><li>MW from 200 Da to 10 MDa</li><li><i>r<sub>g</sub></i> &nbsp;from 10 to 50 nm, up to 150 nm with shape-specific models</li><li>sensitivity down to 25 ng of 100 kDa polystyrene in THF, injected on a standard GPC column</li></ul><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Waters_Mini_DAWN_urcuje_molekulovou_hmotnost_nezavisle_na_kalibraci_kolony_Zde_MALS_ukazuje_ze_nejdrive_eluujici_molekula_nema_nejvyssi_molarni_hmotnost_509e5530ea.jpg" alt="Waters: The miniDAWN determines molecular weight independently of column calibration. Here MALS shows that the earliest-eluting molecule does not have the highest molar mass." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Waters_Mini_DAWN_urcuje_molekulovou_hmotnost_nezavisle_na_kalibraci_kolony_Zde_MALS_ukazuje_ze_nejdrive_eluujici_molekula_nema_nejvyssi_molarni_hmotnost_509e5530ea.jpg 245w," sizes="100vw" width="820" height="438"></p><p>The miniDAWN's performance is exceeded only by the <strong>DAWN™</strong> which covers a wider range of size and molecular weight, with even better sensitivity and additional optional modules.</p><h5><strong>Ultrasonic Cleaning Included</strong></h5><p>Each miniDAWN includes an embedded <strong>COMET™ ultrasonic transducer</strong> that cleans the flow cell automatically by agitating the fluid and loosening particles from the glass surfaces.</p><h5><strong>Modularity = Productivity</strong></h5><p>The miniDAWN incorporates a fully modular design. Each module can be tested independently by the software to verify that it is functioning correctly. This imparts nearly complete field serviceability, since malfunctioning modules can be replaced and validated on-site by service engineers with no further calibration or alignment.</p><p>The miniDAWN is also field-upgradeable: the embedded <strong>WyattQELS™ DLS Module </strong>for measuring hydrodynamic radii from 0.5 to 50 nm can be installed on-site as well as in new units shipped from the factory.</p><h5><strong>Not just SEC-MALS...</strong></h5><p>Wyatt's miniDAWN may be used in batch (off-line) mode or in conjunction with a <strong>Calypso™ CG-MALS system</strong> for characterization of average solution properties as well as macromolecular interaction properties including protein-protein binding affinity and complex stoichiometry.</p><h5><strong>Simplicity personified</strong></h5><p>With miniDAWN and <strong>ASTRA™</strong>, SEC-MALS became a whole lot easier. Sample-specific methods are set up in three short steps with ASTRA's Method Wizard, and new capabilities for automatic data processing mean that it takes just one click from your sample to a full report of the molecular weight and size results.</p><p>The miniDAWN's System Ready monitor will even let you know when the system is fully equilibrated with low baseline noise, ready to run.</p><h4><strong>Specifications</strong></h4><h5><strong>Measurement Capabilities</strong></h5><ul><li><strong>Molar Mass Range:</strong><br>200 Da to 10 MDa for proteins; up to 1 MDa for linear polymers (depending on dn/dc, sample concentration, and chromatography conditions).</li><li><strong>Molecular Size (Radius of Gyration, Rg):</strong><br>Approximately 10 nm to 50 nm, extendable up to 150 nm with shape-specific modeling.</li><li><strong>Hydrodynamic Radius (Rh):</strong><ul><li><strong>Flow mode:</strong> 0.5 nm to 50 nm (optimal at flow rates ≤ 0.3 mL/min)</li><li><strong>Batch mode:</strong> 0.5 nm to 1 µm (requires WyattQELS™ module or DynaPro™ NanoStar™ with fiber optic connection)</li></ul></li><li><strong>Sensitivity:</strong><br>Detects as little as 25 ng of 100 kDa polystyrene in THF or 500 ng of BSA in PBS using a standard 7.8 mm x 300 mm SEC column with no post-column filter.</li></ul><h5><strong>Fluidics and Compatibility</strong></h5><ul><li><strong>Mobile Phase Compatibility:</strong><br>Compatible with both aqueous and organic solvents.</li><li><strong>Wetted Materials:</strong><br>316 stainless steel, fused silica, and Kalrez.</li></ul><h5><strong>Optical System and Detectors</strong></h5><ul><li><strong>MALS Detectors:</strong><br>Three high-gain, high dynamic range photodiodes at fixed angles.</li><li><strong>Auxiliary Detectors:</strong><ul><li>Laser monitor for stability control</li><li>Forward transmission monitor for absorbing samples and data quality evaluation</li></ul></li><li><strong>Dynamic Range:</strong><br>3,300,000:1</li><li><strong>Optional DLS Detection:</strong><ul><li>Integrated WyattQELS™ module within the miniDAWN chassis</li><li>External fiber optic connection from DynaPro™ NanoStar or Mobius™ DLS-ELS instruments</li></ul></li></ul><h5><strong>Laser Specifications</strong></h5><ul><li><strong>Wavelength:</strong><br>658 nm ± 4 nm</li><li><strong>Power Control:</strong><br>Programmable from 10% to 100%</li></ul><h5><strong>Temperature Control</strong></h5><ul><li><strong>Sample Temperature:</strong> Ambient</li></ul><h5><strong>Electronics and Connectivity</strong></h5><ul><li><strong>Analog Inputs:</strong><br>2 differential inputs, 24-bit resolution, ±10 V range</li><li><strong>Analog Outputs:</strong><br>1 output from a selectable measurement channel, ±10 V range</li><li><strong>I/O Ports:</strong><br>Alarm in/out, auto-inject control, Orbit™ recycle valve support</li><li><strong>Computer Interface:</strong><br>Ethernet</li><li><strong>Data Rate:</strong><br>Up to 36.6 Hz</li></ul><h5><strong>User Interface and Display</strong></h5><ul><li><strong>Front Panel:</strong><br>7” (178 mm) high-resolution multi-touch display for real-time signal viewing, diagnostics, and settings</li></ul><h5><strong>Instrument Dimensions</strong></h5><ul><li><strong>Size:</strong> 58 cm (L) x 36 cm (W) x 18 cm (H)</li></ul><h4><strong>Other MALS Instruments</strong></h4><ul><li><a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/products/1114"><strong>DAWN™</strong></a> - The most sensitive MALS detector available, anywhere. Incorporates detectors at 18 angles to determine molar masses from 200 Da to 1 GDa and radii from 10 – 500 nm.<ul><li>Standard option: ambient temperature</li><li>Heated/cooled option: -15 °C to +150 °C</li><li>High-temperature option: ambient to +210 °C</li><li>The DAWN offers special options to handle fluorescent samples: fluorescence-blocking filters and an infrared, 785 nm laser.</li></ul></li><li><a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/products/1116"><strong>microDAWN™</strong></a><strong> </strong>- The first MALS detector for UHPLC, with interdetector dispersion as low as 1.5 µL. Incorporates detectors at 3 angles to determine molar masses from 200 Da to 20 MDa and radii from 10 – 50 nm. Ambient only.</li><li><strong>ultraDAWN™</strong> - Measures multi-angle light scattering and reports the results – weight-average molecular weight <i>M<sub>w</sub></i> &nbsp;and rms radius <i>R<sub>g</sub></i> &nbsp;– in real time.</li></ul>

Waters/Wyatt microDAWN™ Multi Angle Light Scattering (MALS) Detector

The microDAWN can be coupled to any UHPLC-SEC system to determine absolute molecular weights and sizes of polymers, peptides, or proteins directly, without resorting to column calibration or reference standards.

<h4><strong>Overview</strong></h4><h5><strong>UHPLC-SEC System</strong></h5><p>The microDAWN can be coupled to any UHPLC-SEC system to determine absolute molecular weights and sizes of polymers, peptides, or proteins directly, without resorting to column calibration or reference standards. The microDAWN produces minimal band broadening in order to maintain the narrow peaks typical of UHPLC, while meeting or exceeding the sensitivity of the <strong>miniDAWN™</strong>.</p><h4><strong>Product Description</strong></h4><p>The <strong>microDAWN MALS detector</strong> performs absolute characterization of macromolecules eluting from UHPLC, determining molar mass and size <i>independently </i>of retention time and molecular reference standards. This instrument offers superb sensitivity over a wide range of molar mass, size and concentrations. In most respects the microDAWN is similar to the well-established <strong>miniDAWN™</strong>, a workhorse MALS instrument used in hundreds of labs around the world for characterizing proteins and other molecules smaller than 50 nm in radius.</p><p>Distinctly from the miniDAWN, the <strong>microDAWN</strong> is uniquely <strong>suitable for use in conjunction with UHPLC Size Exclusion Chromatography (SEC-MALS)</strong> thanks to its small interdetector dispersion. In some cases it may be used with ion-exchange or other types of chromatography. Regardless of the specifics, the microDAWN produces minimal band broadening in order to maintain the narrow peaks typical of UHPLC.</p><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Waters_micro_DAWN_MALS_HPLC_detektor_konfigurace_1_4e888650cf.jpg" alt="Waters microDAWN™ Multi Angle Light Scattering (MALS) Instrument: Experimental setup" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Waters_micro_DAWN_MALS_HPLC_detektor_konfigurace_1_4e888650cf.jpg 229w," sizes="100vw" width="894" height="609"></p><ul><li><i>M<sub>w</sub>&nbsp;</i> from 200 Da to 10 MDa</li><li><strong>Sensitivity:</strong> 1.0 µg/mL BSA (66.4 kDa) in PBS; 50 ng/mL 100 kDa polystyrene in THF.</li><li><i>R<sub>g</sub></i> &nbsp;from 10 to 50 nm</li><li>Ultra-stable laser</li></ul><p>The microDAWN and its companion<strong> microOptilab™ Refractive Index Detector</strong> interface to most standard UHPLC systems. As these detectors are based on the advanced technology of Wyatt's miniDAWN and <strong>Optilab™</strong>, respectively, users may be confident in the underlying capabilities cited in <strong>thousands of peer-reviewed publications</strong>.</p><h5><strong>Ultrasonic Cleaning Included</strong></h5><p>Each microDAWN includes a <strong>COMET™ ultrasonic transducer</strong> that cleans MALS flow cells automatically by agitating the fluid and loosening particles from the glass surfaces.</p><h4><strong>Specifications</strong></h4><h5><strong>Measurement Capabilities</strong></h5><ul><li><strong>Molar Mass Range:</strong><br>From 200 Da to 10 MDa for proteins; up to 1 MDa for linear polymers<br><i>(Typical, depending on dn/dc, sample concentration, and chromatography conditions)</i></li><li><strong>Molecular or Particle Size (Radius of Gyration, Rg):</strong><br>10 nm to 50 nm, extendable up to 150 nm with shape-specific modeling</li><li><strong>Hydrodynamic Radius (Rh):</strong><ul><li><strong>Flow mode:</strong> 0.5 nm to 30 nm</li><li><strong>Batch mode:</strong> 0.5 nm to 1 µm<br><i>(Requires WyattQELS™ DLS module or DynaPro™ NanoStar™ with fiber optic connection)</i></li></ul></li><li><strong>Sensitivity:</strong><br>Detects as little as 3.5 ng of 100 kDa polystyrene in THF or 70 ng of BSA in PBS, assuming use of a 15 cm UHPLC-SEC column with no post-column filter</li></ul><h5><strong>Optical System and Detectors</strong></h5><ul><li><strong>MALS Detectors:</strong><br>Three high-gain photodiodes with broad dynamic range for multi-angle light scattering</li><li><strong>Dynamic Range:</strong><br>3,300,000:1</li><li><strong>Auxiliary Detectors:</strong><ul><li>Laser monitor for stabilization feedback</li><li>Forward transmission monitor for absorbing samples and data quality assessment</li></ul></li><li><strong>Optional DLS Detection:</strong><ul><li>Integrated WyattQELS™ module within the microDAWN chassis</li><li>External DLS connection via fiber optic from DynaPro™ NanoStar or Mobius™ DLS-ELS instruments</li></ul></li></ul><h5><strong>Laser Specifications</strong></h5><ul><li><strong>Wavelength:</strong><br>658 nm ± 4 nm</li><li><strong>Power Control:</strong><br>Programmable from 10% to 100%</li></ul><h5><strong>Sample Temperature and Flow Characteristics</strong></h5><ul><li><strong>Sample Temperature:</strong> Ambient</li><li><strong>Interdetector Band Broadening:</strong><ul><li>Upstream detector to microDAWN: &lt; 1.5 µL</li><li>Upstream to downstream detector: &lt; 1.6 µL</li></ul></li></ul><h5><strong>Material Compatibility</strong></h5><ul><li><strong>Wetted Materials:</strong><br>316 stainless steel, fused silica, PEEK, PTFE, and MP35N (iron-free alloy)</li></ul><h5><strong>Electronics and Connectivity</strong></h5><ul><li><strong>Analog Inputs:</strong><br>2 differential inputs, 24-bit resolution, ±10 V input range</li><li><strong>Analog Outputs:</strong><br>1 output from a user-selectable measurement channel, ±10 V range</li><li><strong>I/O Ports:</strong><br>Alarm in/out, auto-inject control, Orbit™ valve integration</li><li><strong>Computer Interface:</strong><br>Ethernet</li><li><strong>Data Transmission Rate:</strong><br>Up to 73 Hz</li></ul><h5><strong>User Interface</strong></h5><ul><li><strong>Display:</strong><br>7” (178 mm) high-resolution multi-touch screen for signal monitoring, instrument settings, and diagnostics</li></ul><h5><strong>Instrument Dimensions</strong></h5><ul><li><strong>Size:</strong> 58 cm (L) x 36 cm (W) x 18 cm (H)</li></ul><h4><strong>Other MALS Instruments</strong></h4><ul><li><a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/products/1114"><strong>DAWN™</strong></a> - The most sensitive MALS detector available, anywhere. Incorporates detectors at 18 angles to determine molar masses from 200 Da to 1 GDa and radii from 10 – 500 nm.<ul><li>Standard option: ambient temperature</li><li>Heated/cooled option: -15 °C to +150 °C</li><li>High-temperature option: ambient to +210 °C</li><li>The DAWN offers special options to handle fluorescent samples: fluorescence-blocking filters and an infrared, 785 nm laser.</li></ul></li><li><a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/products/1115"><strong>miniDAWN™</strong></a><strong> -</strong> Second only to the DAWN in sensitivity. Incorporates detectors at 3 angles to determine molar masses from 200 Da to 10 MDa and radii from 10 – 50 nm. Ambient only.</li><li><strong>ultraDAWN™</strong> - Measures multi-angle light scattering and reports the results – weight-average molecular weight <i>M<sub>w</sub></i> &nbsp;and rms radius <i>R<sub>g</sub></i> &nbsp;– in real time.</li></ul>

Waters/Wyatt omniDAWN™ MALS detector

Waters omniDAWN is an advanced MALS detector for UPLC/UHPLC, delivering absolute molecular weight and particle size measurements from 200 Da to 1 GDa.

<p><strong>The omniDAWN™ MALS Detector is a next-generation multi-angle light scattering system specifically designed for UPLC™ and UHPLC workflows. It delivers absolute molecular weight and particle size measurements without relying on column calibration or molecular standards, allowing researchers to obtain highly accurate characterization data at true high-performance chromatography speeds.</strong></p><p>Equipped with 18 optimally positioned scattering angles and a flow cell engineered to minimize stray light, omniDAWN measures molar masses from 200 Da to 1 GDa and particle radii from 10 nm to 1 µm. The system is ideally suited for characterization of proteins, antibody-drug conjugates (ADCs), lipid nanoparticles (LNPs), viral vectors, polymers, and other complex macromolecular systems.</p><p>Combined with ASTRA™ software, omniDAWN provides comprehensive characterization of molecular size, molar mass, composition, and stoichiometry. Integrated health monitoring and the COMET™ ultrasonic cleaning module ensure reliable operation, minimal maintenance, and consistent analytical performance.</p><h3><strong>Key Features</strong></h3><ul><li>Purpose-built for UPLC™ and UHPLC systems</li><li>Absolute molecular weight determination without calibration standards</li><li>Absolute particle size characterization</li><li>18 optimized light scattering angles</li><li>Molecular weight range from 200 Da to 1 GDa</li><li>Particle radius range from 10 nm to 1 µm</li><li>High sensitivity with minimal stray light interference</li><li>Up to 4× faster LC-MALS analysis using UPLC</li><li>Reduced sample consumption</li><li>Improved chromatographic resolution</li><li>Advanced workflows for ADCs, LNPs, viral vectors, and polymers</li><li>ASTRA™ software for advanced data analysis</li><li>Integrated System Ready Monitor</li><li>COMET™ ultrasonic in situ cleaning</li><li>Real-time health diagnostics</li><li>Automated system performance monitoring</li></ul><h3><strong>Technical Specifications</strong></h3><h4><strong>Technology</strong></h4><ul><li>Multi-Angle Light Scattering (MALS)</li><li>18 light scattering detectors</li><li>Low stray-light flow cell design</li></ul><h4><strong>Measurement Range</strong></h4><ul><li><strong>Molecular weight:</strong> 200 Da to 1 GDa</li><li><strong>Particle radius: </strong>10 nm to 1 µm</li></ul><h4><strong>Performance</strong></h4><ul><li>UPLC™ and UHPLC compatible</li><li>Up to 4× faster than conventional HPLC-MALS</li><li>Improved chromatographic resolution</li><li>Reduced sample requirements</li></ul><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Waters_Wyatt_omni_DAWN_MALS_photometer_2_61d44b5eca.jpg" alt="Waters/Wyatt: A) Achieve up to 4× faster LC-MALS runs with UPLC. B) Achieve superior separation, revealing fragment peaks not discernible by HPLC." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Waters_Wyatt_omni_DAWN_MALS_photometer_2_61d44b5eca.jpg 106w," sizes="100vw" width="400" height="588"></p><h4><strong>Software</strong></h4><ul><li>ASTRA™ Software</li><li>Molecular weight analysis</li><li>Particle size determination</li><li>Conjugate stoichiometry analysis</li><li>ADC characterization</li><li>LNP characterization</li><li>Viral vector characterization</li><li>Polymer branching analysis</li></ul><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Waters_Wyatt_omni_DAWN_MALS_photometer_3_635f3c2555.jpg" alt="Waters/Wyatt: ASTRA software goes beyond measuring overall molecular weight by: A) uniquely resolving individual components to quantify protein and conjugated species and determine stoichiometry, and B) reporting the total protein fraction." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Waters_Wyatt_omni_DAWN_MALS_photometer_3_635f3c2555.jpg 244w," sizes="100vw" width="511" height="327"></p><h4><strong>Monitoring and Maintenance</strong></h4><ul><li>System Ready Monitor</li><li>Real-Time Health Monitoring</li><li>COMET™ ultrasonic cleaning</li><li>Automated diagnostics</li><li>Continuous system performance verification</li></ul><h4><strong>Typical Applications</strong></h4><ul><li>Monoclonal antibodies</li><li>Antibody-drug conjugates (ADCs)</li><li>Lipid nanoparticles (LNPs)</li><li>Viral vectors</li><li>Proteins and peptides</li><li>Polymer materials</li><li>Conjugated polymers</li><li>Biotherapeutics</li><li>Nanotechnology research</li></ul>

Products from category MALS detectors - page 1

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