Struggling to accurately measure semi-volatile analytes? Stricter temperature control with HPLC-CAD analysis just might be the solution you need.<img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Thermo_Fisher_Scientific_How_to_Get_More_Accurate_HPLC_Quantitation_of_Semi_Volatiles_1_f2eefd5901.jpeg" alt="Thermo Fisher Scientific How to Get More Accurate HPLC Quantitation of Semi-Volatiles" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Thermo_Fisher_Scientific_How_to_Get_More_Accurate_HPLC_Quantitation_of_Semi_Volatiles_1_f2eefd5901.jpeg 245w," sizes="100vw" width="1024" height="512">The charged aerosol detector (CAD) is cited as one of the most capable near-universal HPLC detectors available because the response is solely based on particle charge and fully independent of chemical structure.One caveat to know is your mobile phase components must be less volatile than the analytes in solution for detection by the CAD. And while all non-volatiles give a similar uniform response, <a target="_blank" rel="noopener noreferrer" href="https://www.thermofisher.com/content/lifetech/global/en/home/industrial/chromatography/chromatography-learning-center/liquid-chromatography-information/hplc-system-components/how-hplc-detectors-work/charged-aerosol-detection/charged-aerosol-detection-cad-performance-tips.html">the response of semi-volatiles</a> depends on volatility and consequently is less predictable.So, the question then becomes is there a way to address this performance difference and make semi-volatile detection more predictable? One approach for charged semi-volatile analytes is to add a volatile ion-pairing agent of the opposite charge in the mobile phase to force non-volatile behavior.However, this method is analyte specific and may also react with impurities in your mobile phase, leading to higher noise and poorer signal-to-noise (S/N) ratio. What’s really needed is a more global approach to improve the accuracy for measuring semi-volatiles.This approach is now achievable with Temperature Coupling Mode (TCM) in the <a target="_blank" rel="noopener noreferrer" href="https://discover-pharma.com/thermo-fisher-upgrades-charged-aerosol-detection-tech-to-improve-complex-drug-analysis/">Thermo Scientific Vanquish CAD P Series</a>.<h3>Why stricter temperature control matters for measuring semi-volatiles</h3>Before we get into the specifics, you need to understand why temperature control in the CAD is critical to quantitative accuracy, especially for semi-volatiles (Figure 1).<img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Thermo_Fisher_Scientific_How_to_Get_More_Accurate_HPLC_Quantitation_of_Semi_Volatiles_2_e33cff4cac.jpeg" alt="Thermo Fisher Scientific: How to Get More Accurate HPLC Quantitation of Semi-Volatiles: Figure 1. Illustration showing how the CAD works." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Thermo_Fisher_Scientific_How_to_Get_More_Accurate_HPLC_Quantitation_of_Semi_Volatiles_2_e33cff4cac.jpeg 245w," sizes="100vw" width="1024" height="512">The two <a target="_blank" rel="noopener noreferrer" href="https://www.thermofisher.com/content/lifetech/global/en/home/industrial/chromatography/chromatography-learning-center/liquid-chromatography-information/hplc-system-components/how-hplc-detectors-work/charged-aerosol-detection.html">most important functional components in the CAD that guide sensitivity</a> are the evaporation tube temperature (EvapT) and the charging detection module (CDM).<ul><li>EvapT plays a crucial role in determining the performance of the detector, and changing this temperature may alter analyte “selectivity”</li><li>The CDM is where particle charge and measurement happen</li></ul>In previous CAD models, the EvapT is adjustable, but the temperature of the CDM is permanently set at 40 °C. This fixed temperature difference between the EvapT and CDM could negatively impact the measurement of semi-volatile compounds that require a lower EvapT.Adding TCM resolves this issue, by linking the EvapT and CDM temperatures, thereby improving the quantitation of semi-volatile species.<h3>Using TCM to help improve quantification of semi-volatiles, like fatty acids</h3>To demonstrate the effectiveness of this new temperature coupling feature, we anlazyed a working solution of four individual fatty acids (FAs) and compared the CAD repsonse with TCM turned on and turned off (Figure 2).<img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Thermo_Fisher_Scientific_How_to_Get_More_Accurate_HPLC_Quantitation_of_Semi_Volatiles_3_da6cf5006c.jpeg" alt="Thermo Fisher Scientific: How to Get More Accurate HPLC Quantitation of Semi-Volatiles: Figure 2. The mean S/N ratio of the FAs analyzed with TCM turned on and off." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Thermo_Fisher_Scientific_How_to_Get_More_Accurate_HPLC_Quantitation_of_Semi_Volatiles_3_da6cf5006c.jpeg 245w," sizes="100vw" width="700" height="410">Notably, we observed 46.8% increase in the S/N at the optimal EvapT of 25 °C and with the TCM switched on for lauric acid, the most semi-volatile compound!Interestingly, the observed S/N improvement matches the respective analyte volatility—lauric acid (lowest MW) shows the greatest improvement, and stearic acid (highest MW) the least.Taken together, these findings show how stricter temperature control in the CAD can allow you to extend the range and improve quantification of semi-volatiles.<h3>Extending this application to impurity analysis of polysorbates</h3>Now that we’ve seen TCM is useful for analyzing fatty acids, let’s consider more complex solutions, like polysorbates.Given the CAD is well-established for determining <a target="_blank" rel="noopener noreferrer" href="https://www.sciencedirect.com/science/article/pii/S0021967324010240?via%3Dihub">polysorbate impurities</a>, and fatty acid impurities are quite abundant in polysorbates, and a logical next step is to use this technique for analyzing these solutions.Afterall, free acids in polysorbates <a target="_blank" rel="noopener noreferrer" href="https://www.thermofisher.com/blog/analyteguru/review-of-analytical-methods-for-quantifying-amp-profiling/">may form insoluble particles</a> that can seriously compromise product quality, efficacy, and patient safety. Thus, your ability to accurately measure these acids in raw materials and finished goods is of the upmost importance.Download our paper to see how the <a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/labrulez-bucket-strapi-h3hsga3/an_003867_hplc_vanquish_cad_gradient_optimized_evap_temp_an003867_na_en_b64411eb47.pdf">Vanquish CAD P Series</a> can help your lab measure fatty acids with greater confidence today.<img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Thermo_Fisher_Scientific_How_to_Get_More_Accurate_HPLC_Quantitation_of_Semi_Volatiles_4_70473e3696.jpeg" alt="Thermo Fisher Scientific: How to Get More Accurate HPLC Quantitation of Semi-Volatiles: Figure 3. Thermo Scientific Vanquish CAD P Series" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Thermo_Fisher_Scientific_How_to_Get_More_Accurate_HPLC_Quantitation_of_Semi_Volatiles_4_70473e3696.jpeg 245w," sizes="100vw" width="700" height="410"><h3>FAQs</h3><ol><li>What are the advantages of using a charged aerosol detector for HPLC analysis?</li></ol>The <a target="_blank" rel="noopener noreferrer" href="https://www.thermofisher.com/content/lifetech/global/en/home/c/b/vanquish-charged-aerosol-detector-p-series.html">charged aerosol detector</a> (CAD) is now recognized as the most capable “universal” detector available and offers several advantages over other UHPLC-based detectors including:<ul><li>Uniform response for all non-volatile analytes (ideal for quantitation when certified standards are unavailable)</li><li>Universal response independent of chemical structure (analyte does not have to form gas phase ions or possess a chromophore)</li><li>High sensitivity at high pg to low ng on column (ideal for drug impurity analysis)</li><li>Wide linear and dynamic range (measure API and impurities in one run)</li><li>Simple calibration curves (no need to deal with complex, unpredictable sigmoidal calibration curves)</li><li>Gradient compatible (inverse gradient available to ensure consistent response throughout the gradient)</li></ul><ol start="2"><li>Why should I use CAD for measuring fatty acid impurities in polysorbates?</li></ol>Fatty acids don’t contain strong chromophores, so HPLC-CAD analysis is the <a target="_blank" rel="noopener noreferrer" href="https://www.thermofisher.com/blog/analyteguru/cad-vs-elsd-which-hplc-detector-is-your-better-option/">ideal choice</a> since the detector response depends on particle charge and is independent of analyte structure.<ol start="3"><li>How does changing the evaporation tube temperature affect the CAD response for semi-volatiles?</li></ol>A high EvapT can <a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/labrulez-bucket-strapi-h3hsga3/tn_72806_uhplc_charged_aerosol_detection_tn72806_en_5979117651/tn-72806-uhplc-charged-aerosol-detection-tn72806-en.pdf">improve sensitivity</a> for non-volatiles by reducing noise but may negatively impact measurement of semi-volatile species. Conversely, a lower EvapT can improve the detection of semi-volatile species, but sensitivity for all analytes may be adversely impacted due to increased noise.Visit us on&nbsp;<a target="_blank" rel="noopener noreferrer" href="https://www.linkedin.com/showcase/72003132/admin/dashboard/">LinkedIn:</a>&nbsp;#HPLC #CAD #biopharma<section><blockquote><h5>Dr. Kimberly M. Lincoln</h5>Dr. Kimberly M. Lincoln is a marketing specialist in the HPLC division at Thermo Fisher Scientific. She holds a Ph.D. in bioinorganic chemistry and a B.S. in chemistry, with professional experience in digital marketing, medical device manufacturing, and chemical synthesis. Kimberly enjoys writing educational chemical content as a guide to help customers solve analytical problems. She is passionate about living a clean lifestyle and keeping the planet healthy for future generations. In her spare time, Kimberly loves to cook, cycle, attend rock concerts, and spend time with her pit bulls.</blockquote></section>

How to Get More Accurate HPLC Quantitation of Semi-Volatiles

We are pleased to announce that a maintenance release is now available for KnowItAll 2026. Maintenance releases are an important way to introduce improvements and bug fixes between major releases to make sure users get the most out of KnowItAll.In Fall 2025, we announced the KnowItAll 2026 major release featuring a new principal component analysis tool, Trendfinder, and various advances for spectral analysis workflows. This is the first update available after that release.<h5>Updates in the 2026.1 maintenance release include:</h5><ul><li>Continued to LC-MS deconvolution improvement</li><li>Updated the DEA regulations rulebase that’s used to automatically categorize structures in databases you build in MineIt according to current Drug Enforcement Agency controlled substance regulations.</li><li>Various bug fixes</li></ul>We encourage users to update now to the most recent version of the software to ensure the best performance.<ul><li><a target="_blank" rel="noopener noreferrer" href="https://get.knowitall.com/Download?_gl=1*18h0vjr*_gcl_au*Mjk3ODY0Mzg1LjE3MTMyNzcyMDEuMTkwNDkyNDI4NS4xNzEzMzY3NDc0LjE3MTMzNjc0Nzk.">Download the most recent version here</a></li><li><a target="_blank" rel="noopener noreferrer" href="https://sciencesolutions.wiley.com/whats-new-in-knowitall-2026/">What's New in KnowItAll 2026</a></li><li><a target="_blank" rel="noopener noreferrer" href="https://sciencesolutions.wiley.com/knowitall-version-history/">KnowItAll version history</a></li></ul><h4>About KnowItAll</h4>KnowItAll Software offers solutions to analyze and manage multiple types of spectral and chemical data in multiple file and instrument formats. KnowItAll's integrated toolsets eliminate the need for multiple software packages and increase overall lab efficiency and staff proficiency. Combined with Wiley’s comprehensive, high-quality spectral reference libraries, it provides an unparalleled solution for fast, reliable spectral analysis. Learn more.<h4>About <a target="_blank" rel="noopener noreferrer" href="https://www.wiley.com/en-ie/">Wiley</a></h4>Wiley (NYSE: WLY) is a global leader in authoritative content and research intelligence for the advancement of scientific discovery, innovation, and learning. With more than 200 years at the center of the scholarly ecosystem, Wiley combines trusted publishing heritage with AI-powered platforms to transform how knowledge is discovered, accessed, and applied. From individual researchers and students to Fortune 500 R&amp;D teams, Wiley enables the transformation of scientific breakthroughs into real-world impact. From knowledge to impact—Wiley is redefining what's possible in science and learning. Visit us at Wiley.com and Investors.Wiley.com. Follow us on Facebook, X, LinkedIn and Instagram.

KnowItAll 2026.1 maintenance release now available - Upgrade for the latest features and fixes

<h3>Introduction</h3>Modern analytical laboratories often operate multiple chromatographic instruments simultaneously, which places high demands on software usability and workspace navigation. <a target="_blank" rel="noopener noreferrer" href="https://gcms.labrulez.com/products/999">Clarity Chromatography Software</a> developed by DataApex provides a structured, instrument-oriented user interface designed to simplify work even in complex multi-instrument environments. This article explains how to efficiently move between windows in Clarity.<h3>Concept of Instrument-Centric Workspace Organization</h3><h4>Instrument-Specific Color Schemes</h4>Each instrument in Clarity is visually distinguished by its own color scheme. This design choice allows users to:<ul><li>Instantly recognize which windows belong to the same instrument</li><li>Avoid confusion when multiple instruments are open simultaneously</li><li>Maintain orientation within complex analytical workflows</li></ul>Color coding is consistent across all windows associated with a given instrument.<h3>Types of Windows in Clarity</h3>Within a single instrument context, Clarity typically provides access to several interconnected windows, including:<ul><li>Instrument Window – the central control and overview window</li><li>Chromatogram Window – displays acquired chromatographic data</li><li>Sequence Window – manages sample sequences and runs</li></ul>Efficient navigation between these windows is essential for smooth operation.<h3>Navigation Methods Between Windows</h3>Clarity offers multiple complementary ways to switch between related windows. Users can choose the method that best fits their workflow preferences.<h4>1. Instrument Window Tiles</h4>Within the Instrument Window, large tiles represent related windows.Example workflow:<ul><li>Select the large chromatogram tile to switch directly from the Instrument Window to the Chromatogram Window</li></ul>This tile-based approach is intuitive and visually guided.<h4>2. Toolbar Icons</h4>Each instrument window contains a toolbar with window icons that provide direct access to other related windows.Key characteristics:<ul><li>The same set of window icons is available across all instrument-related windows</li><li>Enables quick transitions, for example:<ul><li>From Chromatogram Window → Sequence Window</li></ul></li><li>Ideal for experienced users who prefer icon-based navigation</li></ul><h4>3. Window Menu</h4>For users who prefer menu-driven control, Clarity includes a Window menu.Typical use case:<ul><li>Select Main from the Window menu to return to the Clarity main window</li></ul>This method is particularly useful when:<ul><li>Navigating back to the global workspace</li><li>Managing multiple instruments at once</li></ul><h3>Practical Navigation Workflow Example</h3>A typical navigation sequence demonstrated in the video follows this logic:<ol><li>Start in the Instrument Window</li><li>Click the Chromatogram tile to open chromatographic data</li><li>Use the toolbar icon to switch to the Sequence Window</li><li>Open the Window menu and select Main to return to the Clarity main interface</li></ol>This combination of tiles, icons, and menus ensures flexible and efficient workspace control.<h3>Advantages of Clarity’s Navigation Design</h3>The navigation system in Clarity provides several practical benefits:<ul><li>Consistency – identical navigation tools across instrument windows</li><li>Efficiency – rapid switching without losing context</li><li>Scalability – effective even with many instruments open</li><li>User preference flexibility – toolbar or menu-based workflows</li></ul>These features are especially valuable in high-throughput laboratories and 24/7 operational environments.<h3>Conclusion</h3>Clarity Chromatography Software uses a well-structured, instrument-oriented window system that simplifies navigation even in complex multi-instrument setups. By combining color-coded instruments, tile-based access, universal toolbar icons, and traditional menus, Clarity allows users to move quickly and confidently between windows.Mastering these navigation techniques significantly improves workflow efficiency, reduces errors, and enhances overall user experience—making Clarity a robust platform for modern chromatographic laboratories.For additional tutorials and practical demonstrations, users are encouraged to explore further educational resources provided by DataApex.

How to switch between the Clarity Windows

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Bioanalytic Development of LC-MS/MS method

We take an individual and factual approach to developing a new method and performing analyses.

<h4>An experienced partner in the LC-MS/MS analysis industry</h4>If you are interested in performing quantitative, qualitative or mixed analysis using LC-MS/MS analytical methods but lack such capabilities due to lack of equipment, “know-how” or are looking for a team to perform analysis for a project - you have come to the right place. We work on <a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/companies/315">equipment</a> with high sensitivity, speed and specificity. Our team is a group of specialists with comprehensive experience in the industry. The LabExperts <a target="_blank" rel="noopener noreferrer" href="https://labexperts.com.pl/zespol/">team</a> has participated in many scientific projects, R&amp;D and commercial implementations. We take an individual and factual approach to developing a new method and performing analyses:<ul><li>In the first stage, we get to know your requirements - to align expectations with capabilities;</li><li>Next, we will form and approve the framework for cooperation, ensuring the observance of confidentiality by signing a non-disclosure agreement (NDA);</li><li>We will plan together and, for our part, meet the deadlines for the completion of the set tasks;</li><li>We will perform preliminary studies, after which we will decide together whether and to what extent we continue to develop the methods;</li><li>We will handle the development of the sample preparation procedure;</li><li>We will select the best parameters for the LC-MS/MS analytical method;</li><li>The analytical method along with the sample preparation procedure will be validated according to generally accepted guidelines and standards, or we will establish the required acceptance criteria and ranges for your needs;</li><li>We will perform LC-MS/MS analyses of a series of samples;</li><li>We will provide results in the form of easy-to-read reports at each required stage;</li><li>We will evaluate the feasibility and assist in the transfer of the method to your laboratory.</li></ul><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Bioanalytic_Bioanalytic_Development_of_LC_MS_MS_method_2594c9fd54.jpg" alt="Bioanalytic: Bioanalytic Development of LC-MS-MS method." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Bioanalytic_Bioanalytic_Development_of_LC_MS_MS_method_2594c9fd54.jpg 234w," sizes="100vw" width="1536" height="1024">Pricing and the framework for cooperation are always determined individually depending on the scope of the study. This is influenced by the amount of work and the complexity of the development of the sample preparation procedure and analytical method and routine analytics. As a rule, the final price consists of three separately priced elements: the cost of preliminary studies, method validation, and the cost of analysis of one sample within a series of analyses.Don't beat your head against the wall - we will help you achieve your goal!We look forward to working with you!

Bioanalytic Individual LC-MS/MS training

We are ready to prepare customized LC-MS/MS training for the needs of participants.

<h4>BASIC INFORMATION</h4><ul><li>TITLE: Customized LC-MS/MS Training</li><li>CATEGORY: LC-MS/MS training</li><li>LOCATION: Lodz, PL or other</li><li>TRAINING TIME: 1-5 days or more</li><li>NUMBER OF PARTICIPANTS: 4-8 people</li><li>EQUIPMENT: SCIEX QTRAP/QTOF LC-MS/MS</li></ul><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Bioanalytic_Bioanalytic_Individual_LC_MS_MS_training_8a73408c56.jpg" alt="Bioanalytic: Bioanalytic Individual LC-MS-MS training." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Bioanalytic_Bioanalytic_Individual_LC_MS_MS_training_8a73408c56.jpg 245w," sizes="100vw" width="1536" height="930"><h4>TRAINING DESCRIPTION</h4>Customized LC-MS/MS training we are ready to prepare for the needs of participants, discussing:<ul><li>theoretical basics of chromatography, mass spectrometry,</li><li>practical operation of the LC-MS/MS system,</li><li>qualitative and quantitative analysis,</li><li>DDA &amp; DIA screening methods,</li><li>“omics” analyses,</li><li>validation of analytical methods,</li><li>solving basic methodological and technical problems,</li><li>software,</li><li>calibration,</li><li>“tips&amp;tricks”,</li><li>etc...</li></ul>We offer training for both novices starting the fascinating adventure of LC-MS/MS analytics, as well as for those who are LC-MS/MS users and are interested in expanding and consolidating their knowledge.<h4>Framework training program</h4><ul><li>individual LC-MS/MS training is developed according to the customer's demand</li><li>conducting theoretical and/or practical training at the customer's workplace</li><li>carrying out theoretical and/or practical training in a laboratory in Lodz</li></ul>

Bioanalytic Basic LC-MS/MS training

Basic LC-MS/MS training covers the theoretical basics of mass spectrometry and familiarization with the basic operation of the instrument.

<h4>BASIC INFORMATION</h4><ul><li>TITLE: Basic LC-MS/MS training</li><li>CATEGORY: LC-MS/MS training</li><li>LOCATION: Lodz, PL</li><li>TRAINING TIME: 3 days</li><li>NUMBER OF PARTICIPANTS: up to 5 people</li><li>EQUIPMENT: SCIEX QTRAP &amp; QTOF LC-MS/MS</li></ul><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Bioanalytic_Bioanalytic_Basic_LC_MS_MS_training_fb1382a55b.jpg" alt="Bioanalytic: Bioanalytic Basic LC-MS-MS training." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Bioanalytic_Bioanalytic_Basic_LC_MS_MS_training_fb1382a55b.jpg 205w," sizes="100vw" width="1293" height="987"><h4>TRAINING DESCRIPTION</h4>Basic LC-MS/MS training covers the theoretical basics of mass spectrometry and familiarization with the basic operation of the instrument. Basic LC-MS/MS training is primarily aimed at those who are new to LC-MS/MS equipment or who wish to become familiar with this analytical technique. During the training, the following will be presented:<ul><li>principles of developing and optimizing quantitative and qualitative methods on low (triple quadrupole, QTRAP) and high resolution (QTOF) instruments,</li><li>basic functionality of the software,</li><li>procedures and objectives of instrument calibration,</li><li>principles of maintaining the cleanliness of mass spectrometer operation.</li></ul><h5>Basic LC-MS/MS training - the program</h5>Day ITheoretical classes:<ul><li>Introduction to LC</li><li>Introduction to mass spectrometry (basics)</li><li>Quantitative analysis.</li></ul>Practical part:QTRAP:<ul><li>QTRAP scanning modes on the instrument - Q1, , MS2, MRM, demonstration and discussion on the example of a selected pesticide of the fragmentation spectrum and its fragmentation pathway.</li><li>MRM pair formation (manual, semi-automatic, automatic).</li><li>optimization of ion source parameters -T-conector</li><li>explanation of the main parameters related to LC (column selection, gradient vs isocrat, autosampler)</li><li>LC-MS/MS method setup and optimization</li></ul>QTOF:<ul><li>manual and automatic optimization of MRMHR</li><li>ion source and LC parameters (transfer from method developed above)</li></ul>Sequence of quantitative analysis on both instruments.Day IIQuantitative analysis:<ul><li>data processing and analysis,</li><li>reporting - Sciex OS (QTRAP, QTOF).</li><li>Discussion of types of integration algorithms (MQ4, autopeak) and integration parameters.</li><li>independent selection of integration parameters and plotting of master curves.</li><li>independent data analysis and generation of analysis report.</li></ul>Qualitative analysis:<ul><li>Theoretical introduction - introduction to qualitative analysis:</li><li>qwadrupole modes (Prec/NL),</li><li>trap modes (EMS, EPI, MS3),</li><li>QTOF modes (TOF MS, TOF MS/MS).</li><li>mixed methods,</li><li>targeted vs. untargeted analysis,</li><li>DDA (IDA) - discussion of method development,</li></ul>Practical part:<ul><li>creation of qualitative methods on QTRAP (Prec-IDA, MRM-IDA), other parameters (LC, ion source) as the previous day,</li><li>LightSight - discussion of operation, creation of MRM-IDA multimethod for selected pesticide and analysis of samples (night).</li><li>QTOF - creation of IDA method, other parameters (LC, ion source) as the previous day.</li></ul>Sequence of qualitative analysis overnight.Day IIIQualitative data analysis:QTRAP:<ul><li>Discussion of Prec-IDA, MRM-IDA results - what we see in different types of MS and MS/MS scans. Manual review of data and via IDA Explorer (SciexOS).</li><li>Analysis of results in LightSight, comparison with Prec-IDA, for example - advantages/disadvantages</li></ul>QTOF:<ul><li>search for potentially present compounds in the sample,</li><li>manual comparison of blank vs. test sample,</li><li>manual by XIC extraction (by sum formula),</li><li>Formula finder - derivation of sum formula on TOF MS data and verification of fragmentation on TOF MS/MS data</li><li>search in IDA Explorer mode - application of filters: similarity, isotopic distribution, mass defect,</li><li>database searching,</li><li>Molecule Profiler - demonstration of software capabilities on selected pesticide example,</li></ul>Instrument calibration:<ul><li>resolution, mass-to-charge ratio, intensity,</li><li>Q1 and Q3, LIT (MS and MS/MS), TOF MS and TOF-MS/MS</li><li>Automation vs. manual calibration,</li></ul>LC-MS/MS application examplesSummaryDiscussion

Bioanalytic Quantitative LC-MS/MS analysis training

The goal of the training is to introduce users to the procedures and capabilities of the LC-MS/MS system and software to achieve sensitivity, selectivity, precision and reproducibility in quantitative analysis.

<h4>BASIC INFORMATION</h4><ul><li>TITLE: Quantitative LC-MS/MS analysis training</li><li>CATEGORY: LC-MS/MS training</li><li>LOCATION: Lodz, PL</li><li>TRAINING TIME: 3 days</li><li>NUMBER OF PARTICIPANTS: up to 5 people</li><li>EQUIPMENT: SCIEX QTRAP LC-MS/MS</li></ul><figure class="image"><img style="aspect-ratio:1200/675;" src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Bioanalytic_Bioanalytic_Quantitative_LC_MS_MS_analysis_training_d13b5d8127.jpg" alt="Bioanalytic: Bioanalytic Quantitative LC-MS-MS analysis training." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Bioanalytic_Bioanalytic_Quantitative_LC_MS_MS_analysis_training_d13b5d8127.jpg 245w," sizes="100vw" width="1200" height="675"></figure><h4>TRAINING DESCRIPTION</h4>Quantitative LC-MS/MS analysis - the training is designed for laboratory personnel using LC-MS/MS systems for quantitative analysis. The following topics are covered during the training:<ul><li>Triple quadrupole spectrometer calibration</li><li>MRM pair formation and optimization</li><li>Optimization of ion source parameters</li><li>Optimization of chromatographic parameters</li><li>Sample preparation using the example of blood drawn on <a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/products/1283">Mitra®</a> samplers (VAMS)</li><li>Matrix effect, internal standards (ISTD), matrix curves</li><li>Validation of the quantitative method</li><li>Reporting of results</li></ul>The goal of the training is to introduce users to the procedures and capabilities of the LC-MS/MS system and software to achieve sensitivity, selectivity, precision and reproducibility in quantitative analysis while covering the widest possible linear range of concentrations.<h3>Training program</h3><h4>Day I</h4><h5>Theoretical part:</h5>Triple quadrupole structure, ionization, MRM and sMRM scanning, chromatography, multimethods<h5>Practical part:</h5><ul><li>Q1/Q3 calibration (10 Da/s), MS/MS - resolution, m/z, intensity.</li><li>MRM pair formation and optimization (manual, semi-automatic, automatic).</li><li>Ion source optimization - T-concector and FIA</li><li>Gradient vs isocrat, dosing, column selection, temperature selection, other important parameters</li><li>Establishment and optimization of LC-MS/MS method.</li><li>How to take care of MS cleanliness - ionization time programming, multi-way valve, cleaning of ion source and instrument front end</li></ul>Sample preparation in pure solvent (standard curve, QC, solvent, solvent+ISTD, test samples), overnight analysis sequence.<h4>Day II</h4><h5>Practical part - preparation of whole blood samples (Mitra®):</h5><ul><li>Preparation of matrix standard curves</li><li>Preparation of quality control (QC) samples in three concentrations</li><li>Preparation of blank samples (without ISTD and with ISTD).</li><li>Preparation of samples for recovery determination (“pre-spiked” and “post-spiked”)</li><li>Preparation of test samples</li></ul><h5>Theoretical part:</h5><ul><li>Samples in the matrix - discussion of the matrix effect and ways to level it.</li><li>Optimization of sample preparation method and LC-MS/MS method</li></ul><h5>Practical part:</h5><ul><li>Sample extraction from Mitra® samplers for LC-MS/MS analysis.</li><li>Sequence of analysis of prepared samples overnight</li></ul><h4>Day III</h4><h5>Theoretical part:</h5><ul><li>Validation of the quantitative LC-MS/MS method</li></ul><h5>Practical part:</h5><ul><li>Software - data integration algorithms, signal smoothing, weighing.</li><li>Matrix curves vs curves in solvent, influence of ISTD (Sciex OS).</li><li>Method validation - validation criteria, determination of LOD, LLOQ, ULOQ, linearity, working range of the method, repeatability, recovery.</li><li>Preparation of summary validation reports</li><li>Reporting of results from test samples - report vs export of data to Excel.</li><li>Summary and discussion.</li></ul>

Bioanalytic QTRAP vs QTOF Quality analysis training

The training includes a presentation of the basic differences in application and a comparison of the capabilities of QTRAP and QTOF instruments in qualitative analysis.

<h4>BASIC INFORMATION</h4><ul><li>TITLE: QTRAP vs QTOF Quality analysis training</li><li>CATEGORY: LC-MS/MS training</li><li>LOCATION: Lodz, PL</li><li>TRAINING TIME: 3 days</li><li>NUMBER OF PARTICIPANTS: up to 5 people</li><li>EQUIPMENT: SCIEX QTRAP &amp; QTOF LC-MS/MS</li></ul><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Bioanalytic_Bioanalytic_QTRAP_vs_QTOF_Quality_analysis_training_18ca66163f.jpg" alt="Bioanalytic: Bioanalytic QTRAP vs QTOF Quality analysis training." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Bioanalytic_Bioanalytic_QTRAP_vs_QTOF_Quality_analysis_training_18ca66163f.jpg 221w," sizes="100vw" width="1000" height="707"><h4>TRAINING COURSE DESCRIPTION</h4>QTRAP vs QTOF qualitative analysis - the training is aimed at people who perform analyses related to substance identification or metabolite search in their research work. The training includes a presentation of the basic differences in application and a comparison of the capabilities of QTRAP and QTOF instruments in qualitative analysis. In addition to the theoretical session, participants will undergo a course in the development and optimization of DDA (IDA) (QTRAP &amp; QTOF) and DIA (SWATH) (QTOF) methods. Analysis and comparison of results will be conducted using <a target="_blank" rel="noopener noreferrer" href="https://sciex.com/products/software/sciex-os-software">SciexOS</a>, <a target="_blank" rel="noopener noreferrer" href="https://sciex.com/products/software/markerview-software">MarkerView</a>, <a target="_blank" rel="noopener noreferrer" href="https://sciex.com/products/software/lightsight-software">LightSight</a> and <a target="_blank" rel="noopener noreferrer" href="https://sciex.com/products/software/molecule-profiler-software">MoleculeProfiler</a> software.<h5>Training program</h5><h4>Day I</h4><h5>Theoretical part</h5><ul><li>Principle of operation of MS/MS systems</li><li>Differences and capabilities of QTRAP and QTOF</li><li>Comparison of typical analytical procedures and their application</li><li>DDA (IDA) vs DIA (SWATH) methodology.</li><li>Supporting software - LightSight, Molecule Profiler, ACD Labs, MarkerView</li><li>General principles of compound fragmentation</li></ul><h4>Day II</h4>Objective - to develop methods to search for metabolites of a known substance.<h5>QTRAP</h5><ul><li>Development of IDA screening methods (pMRM, Prec/NL, EMS)</li><li>Optimization of LC-MS/MS IDA method</li><li>Data acquisition</li></ul><h5>QTOF</h5><ul><li>Development and optimization of LC-MS/MS IDA and SWATH methods</li><li>Data acquisition</li></ul><h4>Day III</h4>Objective - identification and profiling of metabolites of a known substance<h5>QTRAP data analysis</h5><ul><li>Manual search and identification</li><li>LightSight, MarkerView</li></ul><h5>QTOF data analysis</h5><ul><li>Search and data analysis methods</li><li>Manual search and identification</li><li>IDA vs SWATH</li><li>Automated search and identification - SciexOS, databases</li><li>Molecule Profiler, MarkerView</li></ul>Summary and discussion &nbsp;

Bioanalytic QTOF quantitative and qualitative analysis training

The purpose of the training is to introduce users to the capabilities of the LC-HR-MS/MS (QTOF) system in quantitative and qualitative analysis.

<h4>BASIC INFORMATION</h4><ul><li>TITLE:&nbsp; QTOF quantitative and qualitative analysis training</li><li>CATEGORY: LC-MS/MS training</li><li>LOCATION: Lodz, PL</li><li>TRAINING TIME: 3 days</li><li>NUMBER OF PARTICIPANTS: up to 5 people</li><li>EQUIPMENT: SCIEX QTOF LC-MS/MS</li></ul><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Bioanalytic_Bioanalytic_QTOF_quantitative_and_qualitative_analysis_training_af1620ae8c.jpg" alt="Bioanalytic: Bioanalytic QTOF quantitative and qualitative analysis training." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Bioanalytic_Bioanalytic_QTOF_quantitative_and_qualitative_analysis_training_af1620ae8c.jpg 221w," sizes="100vw" width="1000" height="707"><h4>TRAINING COURSE DESCRIPTION</h4>Qualitative and quantitative analysis of QTOF - the training is designed for laboratory personnel using <a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/library?instrumentationItems=30&amp;mainauthorItems=55">high-resolution (HR) LC-MS/MS</a> systems for qualitative and quantitative analysis. The following topics are covered during the training:<ul><li>QTOF spectrometer calibration</li><li>Targeted and untargeted methods</li><li>Quantitative analysis (TOF MS, MRMhr)</li><li>Qualitative and quantitative analysis - DDA (IDA) vs DIA (SWATH) methodology</li><li>Software and its use in high-resolution data analysis</li><li>Reporting of results</li></ul>The purpose of the training is to introduce users to the capabilities of the LC-HR-MS/MS (QTOF) system in quantitative and qualitative analysis. To point out the advantages of high resolution in identifying or confirming the presence of substances in a sample and also the strengths of modern QTOF systems in parallel quantitative analysis.<h3>Training program</h3><h4>Day I</h4><h5>Theoretical part</h5><ul><li>The principle of operation and capabilities of the QTOF system</li><li>Comparison of typical analytical procedures and their application</li><li>Targeted and quantitative analysis (TOF MS vs MRMhr)</li><li>DDA (IDA) vs DIA (SWATH) methodology.</li><li>Supporting software - Molecule Profiler, ACD Labs, MarkerView</li><li>General principles of compound fragmentation</li></ul><h4>Day II</h4>Objective - to develop methods for quantitative analysis, confirmation or search for metabolites of a known substance, general characterization of the sample.<h5>Practical part:</h5><ul><li>Creation and optimization of the MRMhr targeted method</li><li>Creation and optimization of a non-targeted method IDA</li><li>Creation and optimization of the non-targeted method SWATH</li><li>Data acquisition (overnight)</li></ul><h4>Day III</h4>Objective - quantitative analysis, confirmation of the presence or identification of metabolites of a known substance, general characterization of the sample (identification of compounds present in the sample), profiling.<h5>Practical part:</h5><ul><li>Quantitative analysis - TOF MS vs MRMhr</li><li>Methods for searching and analyzing data</li><li>IDA vs SWATH data</li><li>Manual search and identification</li><li>Automated search and identification - SciexOS, databases</li><li>Molecule Profiler, MarkerView</li><li>Summary and discussion</li></ul>

Products from category Services focused on LC/MS/MS distributed by Bioanalytic - page 1

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