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Although modern agriculture needs to be based on accurate real-time data on soil, plant, and environmental conditions, affordable, sufficiently sensitive, and robust monitoring devices for this purpose are currently missing. According to scientists, low-cost sensors based on new smart materials and printing technologies offer a suitable solution. The results of multidisciplinary research thus far and proposals for the development of these devices have been presented by researchers from Czechia, Great Britain, Spain, and the USA in a review article in the prestigious journal <a target="_blank" rel="noopener noreferrer" href="https://www.nature.com/articles/s41467-026-68778-6#Abs1">Nature Communications</a>.“Sensor technologies for monitoring plant health are still in their infancy. Only simple sensors capable of monitoring basic parameters such as pH, soil moisture, or temperature are commonly available on the market. However, if farmers are to face current challenges, including climate change, and ensure sufficient food for humanity, they need much more effective tools that will enable them to monitor, for example, various pathogens, including bacteria and fungi. In our opinion, a suitable solution is a combination of new smart materials and printing for the production of inexpensive, durable, and scalable materials,” said the article’s first author, David Panáček from CATRIN, who worked on the study during his internship at Imperial College London.<img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/CATRIN_Soucasne_senzory_nestaci_Vedci_navrhuji_novou_cestu_ke_sledovani_zdravi_rostlin_b4014952a3.jpg" alt="CATRIN: David Panáček" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_CATRIN_Soucasne_senzory_nestaci_Vedci_navrhuji_novou_cestu_ke_sledovani_zdravi_rostlin_b4014952a3.jpg 208w," sizes="100vw" width="1536" height="1152">Sensors for monitoring plant health, for example, lag behind similar devices used in human medicine. According to the authors, advances in materials science, which will enable the development of scalable sensor technologies, play a key role in this potential shift. “Printing techniques, including inkjet printing, screen printing, aerosol printing, 3D printing, and direct laser writing, offer flexible ways to produce flexible sensors, large-area sensors, and sensors integrated directly into plants. The review article summarizes recent advances in printable low-dimensional materials for agricultural sensors, analyzes their physico-chemical properties in relation to sensor performance, and discusses key challenges and future opportunities requiring interdisciplinary integration,” said one of the corresponding authors, Michal Otyepka.The research brought together materials scientists, chemists, biologists, sensing experts, and printing technology specialists. Materials research and the use of graphene-based smart materials were led by scientists from CATRIN at Palacký University and VŠB-Technical University of Ostrava. Plant biologists from CATRIN contributed their knowledge of plant development, their responses to stress conditions, and the monitoring of their properties. Researchers from Imperial College London took care of the electronics, while representatives from<a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/companies/36"> CEITEC – VUT</a> focused on printing technologies. The team also included leading experts in sensing from the Catalan Institute of Nanoscience and Nanotechnology in Barcelona, and Joseph Wang of the University of California San Diego, the “father of sensing and electrochemistry,” also contributed his extensive knowledge.“This is a groundbreaking study on this topic, which has managed to attract some truly big names in their respective fields. Last but not least, the study demonstrates that interdisciplinarity at CATRIN gives rise to completely new and promising research topics, such as plant sensing and its use for studying plant-environment interactions and smart agriculture,” added another corresponding author, Nuria De Diego.

Current sensors are not good enough. Scientists propose a new way to monitor plant health

In this episode of the Concentrating on Chromatography podcast, host David Oliva interviews Ainslie Chen about her cutting-edge research on hemoglobin variant testing using liquid chromatography-high resolution mass spectrometry (LC-HR-MS). Ainslie discusses her novel method for separating and identifying hemoglobin subunits with mass shifts less than 1 Da, overcoming critical limitations in conventional clinical assays.Hear firsthand how her team developed an LC-HR-MS workflow using a C4 reversed-phase column, tackled technical challenges (including why C18 columns fell short), and achieved unprecedented sensitivity for variant detection in both adult and neonatal samples. The conversation touches on the potential of mass spectrometry to revolutionize clinical diagnostics, the importance of reproducibility, barriers to adoption in routine labs, and how teamwork drives scientific innovation.Perfect for analytical chemists, clinical laboratorians, and anyone interested in the future of protein variant analysis, this episode also features Ainslie’s advice for early-career scientists and insights from her first ACS poster presentation.<h4>Key Highlights:</h4><ul><li>Motivation behind improving hemoglobinopathy testing &nbsp;</li><li>Technical advantages of LC-HR-MS over gene sequencing and CE &nbsp;</li><li>The crucial role of the C4 column in resolving challenging samples &nbsp;</li><li>Critical parameters for reproducible LC-HR-MS workflows &nbsp;</li><li>Barriers and outlook for clinical adoption of advanced mass spectrometry &nbsp;</li><li>The value of teamwork and mentorship in scientific research &nbsp;</li><li>Direct insights from recent ACS conference experiences</li></ul><h3>Video transcription</h3>In this interview, Ainslie Chen discusses her research on improving the detection and identification of hemoglobin variants using liquid chromatography coupled with high-resolution mass spectrometry (LC-HRMS). The work addresses limitations of conventional hemoglobinopathy testing, which often struggles to resolve very small mass differences between closely related hemoglobin subunits. Because even a single amino-acid substitution can significantly alter hemoglobin structure and function, high analytical resolution is essential for accurate diagnosis of conditions such as anemia and sickle cell disease.Chen explains why her team selected LC-HRMS over established clinical techniques like capillary electrophoresis and gene sequencing. While sequencing offers excellent accuracy, it is time-consuming, costly, and requires extensive sample preparation, making it impractical for routine clinical workflows. Capillary electrophoresis enables subunit separation but lacks the resolving power needed to distinguish variants differing by less than one Dalton. In contrast, high-resolution mass spectrometry provides both structural specificity and sensitivity, enabling confident identification of subtle hemoglobin variants with minimal sample preparation.A key methodological insight from the project was the use of a C4 reversed-phase LC column instead of the more common C18 column. Chen describes how the shorter carbon chain and larger pore size of C4 columns improved protein elution and reduced denaturation, leading to more reproducible separations. Critical parameters for reproducibility included consistent mobile-phase composition (0.1% formic acid in water and acetonitrile), controlled temperature (60 °C for LC, ~320 °C capillary temperature), and stable chromatographic conditions.Beyond hemoglobin analysis, Chen highlights the broader potential of high-resolution mass spectrometry in proteoform analysis and clinical diagnostics, including emerging applications in cancer detection. She acknowledges current barriers to clinical adoption—such as energy demands and instrument infrastructure—but points to advances like miniaturized mass spectrometers as promising steps toward wider use. The interview concludes with advice for young scientists: don’t hesitate to reach out to mentors, embrace collaboration, and view analytical chemistry as a versatile tool that bridges medicine, biology, environmental science, and beyond.<blockquote>This text has been automatically transcribed from a video presentation using AI technology. It may contain inaccuracies and is not guaranteed to be 100% correct.</blockquote><blockquote><h4>Concentrating on Chromatography Podcast</h4>Dive into the frontiers of chromatography, mass spectrometry, and sample preparation with host David Oliva. Each episode features candid conversations with leading researchers, industry innovators, and passionate scientists who are shaping the future of analytical chemistry. From decoding PFAS detection challenges to exploring the latest in AI-assisted liquid chromatography, this show uncovers practical workflows, sustainability breakthroughs, and the real-world impact of separation science. Whether you’re a chromatographer, lab professional, or researcher you'll discover inspiring content!You can find Concentrating on Chromatography Podcast in podcast apps:<ul><li><a target="_blank" rel="noopener noreferrer" href="https://open.spotify.com/show/1wZg67rCjoGju4yhqxI3tL">Spotify</a></li><li><a target="_blank" rel="noopener noreferrer" href="https://podcasts.apple.com/tz/podcast/concentrating-on-chromatography/id1788429185">Apple podcast</a></li><li><a target="_blank" rel="noopener noreferrer" href="https://podscan.fm/podcasts/concentrating-on-chromatography">podscan</a></li></ul>and on <a target="_blank" rel="noopener noreferrer" href="https://www.youtube.com/@ChromatographyTalk/videos">YouTube channel</a></blockquote>

Unlocking Hemoglobin Variant Detection: LC-HR-MS Breakthrough with Ainslie Chen

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The Czech Advanced Technology and Research Institute (CATRIN) at Palacký University is a cutting-edge scientific hub dedicated to advancing research in the fields of nanotechnology, biotechnology, and biomedicine. At its core, CATRIN boasts outstanding scientific teams, featuring international researchers. We emphasize interdisciplinarity, foster global collaborations, and work to translate our research findings into practical applications.

CATRIN

Organomation, founded in 1959, designs and manufactures high-quality nitrogen evaporators and extraction systems. Known for innovation and durability, their lab instruments are used globally for efficient sample preparation with strong customer support.

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KNAUER Eurokat H Ion Exclusion/Ligand Exchange Columns (USP L17)

The Eurokat H ionic form is especially designed for the analysis of organic acids and even complex mixtures of acids, sugars and alcohols as well as sugar alcohols.

Organic acids and alcoholsThe Eurokat H ionic form is especially designed for the analysis of organic acids and even complex mixtures of acids, sugars and alcohols as well as sugar alcohols. It is a cation exchange material with 8 % cross linkage of the styrenedivinylbenzene copolymer. Best results are acieved with aqueous eluents and small amounts of anorganic acids, recommended eluent 0.005 M sulfuric acid. The usage of up to 10 % organic content in the mobile phase is possible.<h5>Properties</h5><ul><li>Sulfonated cross-linked styrenedivinylbenzene copolymer phase for the determination of organic acids, carbohydrates and alcohols</li><li>H-form, 8 % cross linkage</li><li>Cation exchanger, extremly long lasting lifetime when correctly handled, best results with aqueous eluents and small amounts of anorganic acids, recommended eluent 0,01 N sulfuric acid, usage of up to 10 % organic content in the mobile phase is possible</li></ul><h5>Technical Data</h5><ul><li>Sulfonated cross-linked styrenedivinylbenzene copolymer with 8 % cross linkage in the ionic form H, available in 10 μm particle size, pressure stable up to maximum 100 bar</li></ul><h5>Recommended application areas</h5><ul><li>Eurokat H is especially designed for the analysis of organic acids and even complex mixtures of acids, sugars and alcohols as well as sugar alcohols. Due to a differing separation process we recommend the use of these type of columns only in 8 mm ID or 4 mm ID.</li></ul><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/KNAUER_Eurokat_H_Chromatogram_e2c6665a1f.webp" alt="KNAUER: Eurokat H Chromatogram" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_KNAUER_Eurokat_H_Chromatogram_e2c6665a1f.webp 245w," sizes="100vw" width="690" height="281"><blockquote>Tip: Eurokat columns are characterized by an extremely long lifetime if handled properly. Especially pressure shocks and organic solvent contents of more than 10 % in the mobile phase should be avoided absolutely.</blockquote><h3>KNAUER Ion Exclusion and Ligand Exchange Columns</h3>Separation of organic acids, carbohydrates, alcohols on sulfonated cross-linked styrenedivinylbenzene copolymer<ul><li>Modifications<ul><li>Eurokat H</li><li>Eurokat Ca</li><li>Eurokat Pb</li><li>Eurokat Na</li></ul></li><li>High Temperature Methods: Up to 90 °C</li><li>Method Development: Optional up to 10 % organic solvent</li><li>Chemically stable: pH 1–12</li><li>Sustainability: Aqueous eluents protect the environment and are cost-effectiv</li><li>User Friendly: Simple and robust methods for daily analysis. Versatile in its use.</li><li>Simplicity: Easy purchase via our online store with fast delivery.&nbsp;</li></ul><h4>Characteristics&nbsp;</h4>Eurokat high performance polymer phases were especially developed for the separation of organic acids, carbohydrates, alcohols and even complex mixtures of these compounds. Eurokat is a sulfonated cross-linked styrenedivinylbenzene copolymer available in several ionic forms (H, Ca and Pb). This particular cation exchanger is characterized by an outstanding density of functional groups, making it the ideal choice for your ion exclusion, size exclusion and ligand exchange chromatography.<h4>Choice of chromatographic conditions&nbsp;</h4>The Eurokat stationary phase is designed to be applicable to a wide range of diverse chromatographic conditions. All Eurokat columns can be used at temperatures up to 90 °C with no organic solvents. The best separation of sugars can be achieved using between 60 to 90 °C. To extend separation performance, it is possible to connect up to three columns of Eurokat in series. Eurokat columns require no organic solvents and thus are environmentally friendly.&nbsp;<h4>Stability&nbsp;</h4>Eurokat polymer columns are extremely stable over the whole pH range. This is one striking advantage compared with silica-based phases which have a limited lifetime at pH extremes, especially in the higher pH range. Most importantly, Eurokat phases show extraordinary column lifetime stability and are not affected by aqueous solvents.&nbsp;<h4>Application areas&nbsp;</h4><ul><li>Carbohydrates and organic acids in softdrinks and fruit juices&nbsp;</li><li>Sugar substitutes</li><li>Food preservatives</li><li>Dairy products</li><li>Urine analytic (Uric acid, Hippuric acid)</li><li>Monitoring of fermentation processes</li></ul><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/KNAUER_Principle_of_ligand_exchange_mechanism_a5b7c21c43.jpg" alt="KNAUER: Eurokat - Principle of ligand exchange mechanism" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_KNAUER_Principle_of_ligand_exchange_mechanism_a5b7c21c43.jpg 245w," sizes="100vw" width="1382" height="570">

KNAUER Eurokat Pb & Ca Ion Exclusion/Ligand Exchange Columns (USP L34 / USP L19)

The ionic forms Ca and Pb are best suited for the analysis of smaller carbohydrates like mono- and disaccharides and sugars up to a degree of polymerization (DP) of 4. 

Analysis of mono- and disaccharidesThe ionic forms Ca and Pb are best suited for the analysis of smaller carbohydrates like mono- and disaccharides and sugars up to a degree of polymerization (DP) of 4. It is a cation exchange material with 6 % cross linkage of the styrenedivinylbenzene copolymer. Best results are achieved with aqueous eluents, recommended eluent is pure deionized water. The usage of up to 10 % organic content in the mobile phase is possible.<h5>Properties</h5><ul><li>Polymer phase for the determination of mono- and disaccharides</li><li>Pb (USP L34) form, 6 % cross linkage</li><li>Cation exchanger, extremely long lasting lifetime when correctly handled, best results with aqueous eluents, recommended eluent is pure deionized water, usage of up to 10 % organic content in the mobile phase is possible</li></ul><h5>Technical Data</h5><ul><li>Sulfonated cross-linked styrenedivinylbenzene copolymer with 6 % cross linkage in the ionic form Pb, available in 10μm particle size, pressure stable up to maximum 100 bar</li></ul><h5>Recommended application areas</h5><ul><li>Especially designed for the analysis of small carbohydrates up to DP 4</li></ul><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/KNAUER_Eurokat_Pb_Ca_Chromatogram_2054f70e49.webp" alt="KNAUER: Eurokat Pb &amp; Ca Chromatogram" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_KNAUER_Eurokat_Pb_Ca_Chromatogram_2054f70e49.webp 245w," sizes="100vw" width="690" height="271"><blockquote>Tip: Eurokat columns are used at high temperatures. During equilibration it is very important to have an eye on the backpressure and to start with very low flow rates and increase them only slowly and stepwise after the column has adapted to the temperature.</blockquote><h3>KNAUER Ion Exclusion and Ligand Exchange Columns</h3>Separation of organic acids, carbohydrates, alcohols on sulfonated cross-linked styrenedivinylbenzene copolymer<ul><li>Modifications<ul><li>Eurokat H</li><li>Eurokat Ca</li><li>Eurokat Pb</li><li>Eurokat Na</li></ul></li><li>High Temperature Methods: Up to 90 °C</li><li>Method Development: Optional up to 10 % organic solvent</li><li>Chemically stable: pH 1–12</li><li>Sustainability: Aqueous eluents protect the environment and are cost-effectiv</li><li>User Friendly: Simple and robust methods for daily analysis. Versatile in its use.</li><li>Simplicity: Easy purchase via our online store with fast delivery.&nbsp;</li></ul><h4>Characteristics&nbsp;</h4>Eurokat high performance polymer phases were especially developed for the separation of organic acids, carbohydrates, alcohols and even complex mixtures of these compounds. Eurokat is a sulfonated cross-linked styrenedivinylbenzene copolymer available in several ionic forms (H, Ca and Pb). This particular cation exchanger is characterized by an outstanding density of functional groups, making it the ideal choice for your ion exclusion, size exclusion and ligand exchange chromatography.<h4>Choice of chromatographic conditions&nbsp;</h4>The Eurokat stationary phase is designed to be applicable to a wide range of diverse chromatographic conditions. All Eurokat columns can be used at temperatures up to 90 °C with no organic solvents. The best separation of sugars can be achieved using between 60 to 90 °C. To extend separation performance, it is possible to connect up to three columns of Eurokat in series. Eurokat columns require no organic solvents and thus are environmentally friendly.&nbsp;<h4>Stability&nbsp;</h4>Eurokat polymer columns are extremely stable over the whole pH range. This is one striking advantage compared with silica-based phases which have a limited lifetime at pH extremes, especially in the higher pH range. Most importantly, Eurokat phases show extraordinary column lifetime stability and are not affected by aqueous solvents.&nbsp;<h4>Application areas&nbsp;</h4><ul><li>Carbohydrates and organic acids in softdrinks and fruit juices&nbsp;</li><li>Sugar substitutes</li><li>Food preservatives</li><li>Dairy products</li><li>Urine analytic (Uric acid, Hippuric acid)</li><li>Monitoring of fermentation processes</li></ul><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/KNAUER_Principle_of_ligand_exchange_mechanism_a5b7c21c43.jpg" alt="KNAUER: Eurokat - Principle of ligand exchange mechanism" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_KNAUER_Principle_of_ligand_exchange_mechanism_a5b7c21c43.jpg 245w," sizes="100vw" width="1382" height="570">

KNAUER Eurokat Na Ion Exclusion/Ligand Exchange Columns

The ionic form Na is best suited for the analysis of sugars oligomers up to a degree of polymerization (DP) of 8. 

Analysis of sugar oligomersThe ionic form Na is best suited for the analysis of sugars oligomers up to a degree of polymerization (DP) of 8. It is a cation exchange material with 6 % cross linkage of the styrenedivinylbenzene copolymer. Best results are achieved with aqueous eluents, recommended eluent is pure deionized water. The usage of up to 10 % organic content in the mobile phase is possible.&nbsp;<h5>Properties</h5><ul><li>Polymer phase for the determination of sugar oligomers</li><li>Na form, 6 % cross linkage</li><li>Cation exchanger, extremly long lasting lifetime when correctly handled, best results with aqueous eluents, recommended eluent is pure deionized water, usage of up to 10 % organic content in the mobile phase is possible</li></ul><h5>Technical Data</h5><ul><li>Sulfonated cross-linked styrenedivinylbenzene copolymer with 6 % cross linkage in the ionic form Na, available in 10 μm particle size, pressure stable up to maximum 100 bar</li></ul><h5>Recommended application areas</h5><ul><li>Especially designed for the analysis of sugar oligomers, separates carbohydrates up to DP 8</li></ul><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/KNAUER_Eurokat_Na_Chromatogram_7d3d81fa52.webp" alt="KNAUER: Eurokat Na Chromatogram" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_KNAUER_Eurokat_Na_Chromatogram_7d3d81fa52.webp 188w," sizes="100vw" width="686" height="568"><blockquote>Tip: Eurokat columns are typically used with 100 % aqueous eluents. For storage, please avoid microbial growth by flushing the Eurokat column with 0.45 µm filtered ultrapure water and storing it at 4 °C.</blockquote><h3>KNAUER Ion Exclusion and Ligand Exchange Columns</h3>Separation of organic acids, carbohydrates, alcohols on sulfonated cross-linked styrenedivinylbenzene copolymer<ul><li>Modifications<ul><li>Eurokat H</li><li>Eurokat Ca</li><li>Eurokat Pb</li><li>Eurokat Na</li></ul></li><li>High Temperature Methods: Up to 90 °C</li><li>Method Development: Optional up to 10 % organic solvent</li><li>Chemically stable: pH 1–12</li><li>Sustainability: Aqueous eluents protect the environment and are cost-effectiv</li><li>User Friendly: Simple and robust methods for daily analysis. Versatile in its use.</li><li>Simplicity: Easy purchase via our online store with fast delivery.&nbsp;</li></ul><h4>Characteristics&nbsp;</h4>Eurokat high performance polymer phases were especially developed for the separation of organic acids, carbohydrates, alcohols and even complex mixtures of these compounds. Eurokat is a sulfonated cross-linked styrenedivinylbenzene copolymer available in several ionic forms (H, Ca and Pb). This particular cation exchanger is characterized by an outstanding density of functional groups, making it the ideal choice for your ion exclusion, size exclusion and ligand exchange chromatography.<h4>Choice of chromatographic conditions&nbsp;</h4>The Eurokat stationary phase is designed to be applicable to a wide range of diverse chromatographic conditions. All Eurokat columns can be used at temperatures up to 90 °C with no organic solvents. The best separation of sugars can be achieved using between 60 to 90 °C. To extend separation performance, it is possible to connect up to three columns of Eurokat in series. Eurokat columns require no organic solvents and thus are environmentally friendly.&nbsp;<h4>Stability&nbsp;</h4>Eurokat polymer columns are extremely stable over the whole pH range. This is one striking advantage compared with silica-based phases which have a limited lifetime at pH extremes, especially in the higher pH range. Most importantly, Eurokat phases show extraordinary column lifetime stability and are not affected by aqueous solvents.&nbsp;<h4>Application areas&nbsp;</h4><ul><li>Carbohydrates and organic acids in softdrinks and fruit juices&nbsp;</li><li>Sugar substitutes</li><li>Food preservatives</li><li>Dairy products</li><li>Urine analytic (Uric acid, Hippuric acid)</li><li>Monitoring of fermentation processes</li></ul><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/KNAUER_Principle_of_ligand_exchange_mechanism_a5b7c21c43.jpg" alt="KNAUER: Eurokat - Principle of ligand exchange mechanism" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_KNAUER_Principle_of_ligand_exchange_mechanism_a5b7c21c43.jpg 245w," sizes="100vw" width="1382" height="570">

Products from category IEX HPLC columns focused on LC columns distributed by KNAUER - page 1

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Unlocking Hemoglobin Variant Detection: LC-HR-MS Breakthrough with Ainslie Chen

Current sensors are not good enough. Scientists propose a new way to monitor plant health

Meeting EU Drinking Water Standards: The Critical Role of Sample Prep in Trace Contaminant Analysis