KNAUER Eurokat H Ion Exclusion/Ligand Exchange Columns (USP L17)

Organic acids and alcohols

The Eurokat H ionic form is especially designed for the analysis of organic acids and even complex mixtures of acids, sugars and alcohols as well as sugar alcohols. It is a cation exchange material with 8 % cross linkage of the styrenedivinylbenzene copolymer. Best results are acieved with aqueous eluents and small amounts of anorganic acids, recommended eluent 0.005 M sulfuric acid. The usage of up to 10 % organic content in the mobile phase is possible.

Properties

• Sulfonated cross-linked styrenedivinylbenzene copolymer phase for the determination of organic acids, carbohydrates and alcohols
• H-form, 8 % cross linkage
• Cation exchanger, extremly long lasting lifetime when correctly handled, best results with aqueous eluents and small amounts of anorganic acids, recommended eluent 0,01 N sulfuric acid, usage of up to 10 % organic content in the mobile phase is possible

Technical Data

• Sulfonated cross-linked styrenedivinylbenzene copolymer with 8 % cross linkage in the ionic form H, available in 10 μm particle size, pressure stable up to maximum 100 bar

Recommended application areas

• Eurokat H is especially designed for the analysis of organic acids and even complex mixtures of acids, sugars and alcohols as well as sugar alcohols. Due to a differing separation process we recommend the use of these type of columns only in 8 mm ID or 4 mm ID.

KNAUER: Eurokat H Chromatogram

Tip: Eurokat columns are characterized by an extremely long lifetime if handled properly. Especially pressure shocks and organic solvent contents of more than 10 % in the mobile phase should be avoided absolutely.

KNAUER Ion Exclusion and Ligand Exchange Columns

Separation of organic acids, carbohydrates, alcohols on sulfonated cross-linked styrenedivinylbenzene copolymer

• Modifications
  • Eurokat H
  • Eurokat Ca
  • Eurokat Pb
  • Eurokat Na
• High Temperature Methods: Up to 90 °C
• Method Development: Optional up to 10 % organic solvent
• Chemically stable: pH 1–12
• Sustainability: Aqueous eluents protect the environment and are cost-effectiv
• User Friendly: Simple and robust methods for daily analysis. Versatile in its use.
• Simplicity: Easy purchase via our online store with fast delivery. 

Characteristics 

Eurokat high performance polymer phases were especially developed for the separation of organic acids, carbohydrates, alcohols and even complex mixtures of these compounds. Eurokat is a sulfonated cross-linked styrenedivinylbenzene copolymer available in several ionic forms (H, Ca and Pb). This particular cation exchanger is characterized by an outstanding density of functional groups, making it the ideal choice for your ion exclusion, size exclusion and ligand exchange chromatography.

Choice of chromatographic conditions 

The Eurokat stationary phase is designed to be applicable to a wide range of diverse chromatographic conditions. All Eurokat columns can be used at temperatures up to 90 °C with no organic solvents. The best separation of sugars can be achieved using between 60 to 90 °C. To extend separation performance, it is possible to connect up to three columns of Eurokat in series. Eurokat columns require no organic solvents and thus are environmentally friendly. 

Stability 

Eurokat polymer columns are extremely stable over the whole pH range. This is one striking advantage compared with silica-based phases which have a limited lifetime at pH extremes, especially in the higher pH range. Most importantly, Eurokat phases show extraordinary column lifetime stability and are not affected by aqueous solvents. 

Application areas 

• Carbohydrates and organic acids in softdrinks and fruit juices 
• Sugar substitutes
• Food preservatives
• Dairy products
• Urine analytic (Uric acid, Hippuric acid)
• Monitoring of fermentation processes

KNAUER: Eurokat - Principle of ligand exchange mechanism