KNAUER Eurokat Pb & Ca Ion Exclusion/Ligand Exchange Columns (USP L34 / USP L19)

Analysis of mono- and disaccharides

The ionic forms Ca and Pb are best suited for the analysis of smaller carbohydrates like mono- and disaccharides and sugars up to a degree of polymerization (DP) of 4. It is a cation exchange material with 6 % cross linkage of the styrenedivinylbenzene copolymer. Best results are achieved with aqueous eluents, recommended eluent is pure deionized water. The usage of up to 10 % organic content in the mobile phase is possible.

Properties

• Polymer phase for the determination of mono- and disaccharides
• Pb (USP L34) form, 6 % cross linkage
• Cation exchanger, extremely long lasting lifetime when correctly handled, best results with aqueous eluents, recommended eluent is pure deionized water, usage of up to 10 % organic content in the mobile phase is possible

Technical Data

• Sulfonated cross-linked styrenedivinylbenzene copolymer with 6 % cross linkage in the ionic form Pb, available in 10μm particle size, pressure stable up to maximum 100 bar

Recommended application areas

• Especially designed for the analysis of small carbohydrates up to DP 4

KNAUER: Eurokat Pb & Ca Chromatogram

Tip: Eurokat columns are used at high temperatures. During equilibration it is very important to have an eye on the backpressure and to start with very low flow rates and increase them only slowly and stepwise after the column has adapted to the temperature.

KNAUER Ion Exclusion and Ligand Exchange Columns

Separation of organic acids, carbohydrates, alcohols on sulfonated cross-linked styrenedivinylbenzene copolymer

• Modifications
  • Eurokat H
  • Eurokat Ca
  • Eurokat Pb
  • Eurokat Na
• High Temperature Methods: Up to 90 °C
• Method Development: Optional up to 10 % organic solvent
• Chemically stable: pH 1–12
• Sustainability: Aqueous eluents protect the environment and are cost-effectiv
• User Friendly: Simple and robust methods for daily analysis. Versatile in its use.
• Simplicity: Easy purchase via our online store with fast delivery. 

Characteristics 

Eurokat high performance polymer phases were especially developed for the separation of organic acids, carbohydrates, alcohols and even complex mixtures of these compounds. Eurokat is a sulfonated cross-linked styrenedivinylbenzene copolymer available in several ionic forms (H, Ca and Pb). This particular cation exchanger is characterized by an outstanding density of functional groups, making it the ideal choice for your ion exclusion, size exclusion and ligand exchange chromatography.

Choice of chromatographic conditions 

The Eurokat stationary phase is designed to be applicable to a wide range of diverse chromatographic conditions. All Eurokat columns can be used at temperatures up to 90 °C with no organic solvents. The best separation of sugars can be achieved using between 60 to 90 °C. To extend separation performance, it is possible to connect up to three columns of Eurokat in series. Eurokat columns require no organic solvents and thus are environmentally friendly. 

Stability 

Eurokat polymer columns are extremely stable over the whole pH range. This is one striking advantage compared with silica-based phases which have a limited lifetime at pH extremes, especially in the higher pH range. Most importantly, Eurokat phases show extraordinary column lifetime stability and are not affected by aqueous solvents. 

Application areas 

• Carbohydrates and organic acids in softdrinks and fruit juices 
• Sugar substitutes
• Food preservatives
• Dairy products
• Urine analytic (Uric acid, Hippuric acid)
• Monitoring of fermentation processes

KNAUER: Eurokat - Principle of ligand exchange mechanism