Scientists at IOCB Prague are uncovering new details of gene transcription. They have identified a previously unknown molecular mechanism by which the transcription of genetic information from deoxyribonucleic acid (DNA) into ribonucleic acid (RNA) can be initiated. The researchers focused on a specific class of molecules known as alarmones, which are found in cells across a wide range of organisms and whose levels often increase under conditions of cellular stress. The results were published in the prestigious scientific journal Nature Chemical Biology.<img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/UOCHB_Vyzkum_z_UOCHB_odhaluje_dosud_nepoznany_zpusob_prepisu_geneticke_informace_bc2bfffa6c.jpg" alt="IOCB Prague/Tomáš Belloň: Research from IOCB Prague reveals a previously unknown mechanism of genetic transcription: Dr. Hana Cahová, head of the Chemical Biology of Nucleic Acids research group." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_UOCHB_Vyzkum_z_UOCHB_odhaluje_dosud_nepoznany_zpusob_prepisu_geneticke_informace_bc2bfffa6c.jpg 234w," sizes="100vw" width="2000" height="1335"><div>Molecules of ribonucleic acid can carry a variety of chemical modifications at one end, known as caps. In eukaryotic organisms, including human cells, the best-known cap plays an important role in RNA stability and in regulating its subsequent fate. In recent years, however, it has become clear that alternative, non-canonical RNA caps also exist, although their formation and the mechanisms by which they are attached to RNA remain only partially understood. These include alarmone caps, formed by dinucleoside polyphosphate molecules, that protect cellular RNA at moments when the cell is under threat.In their study, <a target="_blank" rel="noopener noreferrer" href="https://cahova.group.uochb.cz/en">Dr. Hana Cahová</a> and colleagues examined bacterial RNA polymerase and investigated how this enzyme can initiate transcription using dinucleoside polyphosphates (NpNs) instead of the standard RNA building blocks. For the first time, the scientists described, at the atomic level, how RNA bearing an alarmone cap can be generated directly at the initiation of gene transcription. They also observed that NpNs bind through a different type of base pairing than is typically observed.The work was significantly shaped by Valentina Serianni from Hana Cahová’s team, who demonstrated the ability of dinucleoside polyphosphates to initiate gene transcription, and by Jana Škerlová, who focused on the structural analysis of RNA polymerase. Using cryogenic electron microscopy data, she showed how dinucleoside polyphosphate molecules bind within the active site of RNA polymerase – that is, the core of the enzyme where genetic information is transcribed. Together, their findings help to elucidate the processes that accompany the transcription of genetic information from DNA to RNA.Hana Cahová adds: “We’re describing something that truly occurs in cells and that we’re now able to observe directly at the level of individual molecules. This allows us to answer fundamental questions about cellular processes, such as how cells adapt to stress. RNA plays a central role in this, as it carries the cascade of information underlying any cellular response – for example, to threatening conditions caused by nutrient deprivation or temperature shock.”<img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/UOCHB_Vyzkum_z_UOCHB_odhaluje_dosud_nepoznany_zpusob_prepisu_geneticke_informace_2_0fe88ff9e9.jpg" alt="IOCB Prague/Tomáš Belloň: Research from IOCB Prague reveals a previously unknown mechanism of genetic transcription: Dr. Tomáš Kouba, head of the Cryogenic Electron Microscopy core facility." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_UOCHB_Vyzkum_z_UOCHB_odhaluje_dosud_nepoznany_zpusob_prepisu_geneticke_informace_2_0fe88ff9e9.jpg 234w," sizes="100vw" width="2000" height="1335"><div>Cryogenic electron microscopy (cryo-EM) was central to the project. This part of the work was led by Dr. Tomáš Kouba, one of the authors of the study. “Cryogenic electron microscopy allows us to freeze biological molecules in a state very close to their natural form and then determine their three-dimensional structure. This makes it possible to look directly into the active centers of enzymes and observe their function down to the atomic level,” he explains.Last year, IOCB Prague opened a new <a target="_blank" rel="noopener noreferrer" href="https://cryoem.group.uochb.cz/en">cryo-EM center</a> that is unique within the Czech research environment. State-of-the-art cryogenic electron microscopes are housed in a specially designed building and enable the study of biological processes with exceptional structural precision.<img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/UOCHB_Vyzkum_z_UOCHB_odhaluje_dosud_nepoznany_zpusob_prepisu_geneticke_informace_3_4d881e7a58.jpg" alt="IOCB Prague: Research from IOCB Prague reveals a previously unknown mechanism of genetic transcription: This work shows how NpnNs initiate bacterial transcription. Cryo-EM reveals how nucleobases pair with the template: one in canonical and one in reverse Watson–Crick mode, providing a structural basis for their function as non-canonical RNA caps." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_UOCHB_Vyzkum_z_UOCHB_odhaluje_dosud_nepoznany_zpusob_prepisu_geneticke_informace_3_4d881e7a58.jpg 245w," sizes="100vw" width="2000" height="1077"><blockquote><h4>The Original article</h4><h4><a target="_blank" rel="noopener noreferrer" href="https://www.nature.com/articles/s41589-025-02134-5">Molecular insight into 5′ RNA capping with NpnNs by bacterial RNA polymerase</a></h4>Valentina M. Serianni, Jana Škerlová, Anna Knopp Dubánková, Anton Škríba, Hana Šváchová, Tereza Vučková, Anatolij Filimoněnko, Milan Fábry, Pavlína Řezáčová, Tomáš Kouba &amp; Hana Cahova&nbsp;Nat. Chem. Biol. 21 (2026)<a target="_blank" rel="noopener noreferrer" href="https://doi.org/10.1038/s41589-025-02134-5">https://doi.org/10.1038/s41589-025-02134-5</a><h6>licensed under <a target="_blank" rel="noopener noreferrer" href="https://creativecommons.org/licenses/by/4.0/">CC-BY 4.0</a></h6></blockquote><h4>Abstract</h4>RNA capped with dinucleoside polyphosphates has been discovered in bacteria and eukaryotes only recently. The likely mechanism of this specific capping involves direct incorporation of dinucleoside polyphosphates by RNA polymerase as noncanonical initiating nucleotides. However, how these compounds bind into the active site of RNA polymerase during transcription initiation is unknown. Here, we explored transcription initiation in vitro, using a series of DNA templates in combination with dinucleoside polyphosphates and model RNA polymerase from Thermus thermophilus. We observed that the transcription start site can vary on the basis of the compatibility of the specific template and dinucleoside polyphosphate. Cryo-electron microscopy structures of transcription initiation complexes with dinucleoside polyphosphates revealed that both nucleobase moieties can pair with the DNA template. The first encoded nucleotide pairs in a canonical Watson–Crick manner, whereas the second nucleobase pairs noncanonically in a reverse Watson–Crick manner. Our work provides a structural explanation of how dinucleoside polyphosphates initiate RNA transcription.</div></div>

Research from IOCB Prague reveals a previously unknown mechanism of genetic transcription

Food adulteration is a phenomenon with a long history that has gained increasing importance in recent years due to the globalization of food supply chains and strong economic pressures. Whether it involves diluting olive oil with cheaper oils, adding sugar to honey, or the hidden use of substitutes in protein supplements, the result is a loss of consumer trust and, in some cases, a potential risk to health. Analytical chemistry plays a key role in this area by providing methods and technologies capable of identifying and quantifying target compounds as well as unwanted impurities in a wide range of food matrices.<img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Watrex_Praha_Falsovani_potravin_a_jak_ho_odhali_analyticka_chemie_6912db5492.jpg" alt="Watrex: Food Adulteration and How It Is Revealed by Analytical Chemistry" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Watrex_Praha_Falsovani_potravin_a_jak_ho_odhali_analyticka_chemie_6912db5492.jpg 245w," sizes="100vw" width="1600" height="900"><h4>The Role of Chromatography in Forensic Food Analysis</h4>Chromatography, particularly liquid chromatography (HPLC), is one of the most effective analytical tools for separating complex mixtures of molecules. It enables:<ul><li>qualitative identification of individual components in a sample,</li><li>quantitative determination of their concentrations,</li><li>detection of impurities and unexpected components that may indicate adulteration.</li></ul>In practice, HPLC is often combined with various types of detectors (UV/VIS, DAD, refractive index detectors) or with other techniques (e.g., mass spectrometry) to increase selectivity and sensitivity.<h4>Isocratic vs. Gradient Elution</h4>In the analysis of food samples, chromatographic methods are selected according to the analytical goal:<ul><li>Isocratic HPLC uses a constant mobile phase composition throughout the analysis. It is simple, robust, and ideal for routine determinations of well-defined target analytes (e.g., sugars or vitamins).</li><li>Gradient HPLC changes the mobile phase composition during separation, enabling better resolution of complex mixtures with a wide range of polarities (e.g., peptides or phenolic compounds).</li></ul>Gradient methods are particularly useful when analytes have very different properties and high separation efficiency is required.<h4>Watrex Praha and the Contribution of Modern Systems to Food Analysis</h4><a target="_blank" rel="noopener noreferrer" href="https://watrex.com/?utm_source=labrulez&amp;utm_medium=propagace&amp;utm_campaign=labrulez_clanky_HPLCsystemy">Watrex Praha, s.r.o.</a> is one of the leading Czech manufacturers and distributors of liquid chromatography systems and instruments, with a long tradition dating back to 1990. The company has successfully transformed from a distributor into a manufacturer of its own HPLC and related technologies, focusing on modular, robust systems suitable even for demanding analytical tasks.Their <a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/products?manufacturerItems=93&amp;search=streamline">Streamline™ product line</a> includes several HPLC system concepts suitable for food analysis:<ul><li>Isocratic HPLC system – a simple yet precise configuration with a single pump, column thermostat, and UV/VIS detector; suitable for routine determination of known analytes (e.g., basic carbohydrates, food additives).</li><li>Binary gradient HPLC system – equipped with two pumps enabling mobile phase gradient formation, which enhances separation performance for complex mixtures (e.g., phenolic profiles of wine, components of aromatic extracts).</li><li>GPC/SEC system (Gel Permeation / Size Exclusion Chromatography) – a specialized solution for the determination of macromolecules and polymers, also relevant for the analysis of high-molecular-weight components in foods (e.g., polysaccharides).</li></ul><blockquote>👉<a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/labrulez-bucket-strapi-h3hsga3/Watrex_catalog_2024_ba97395f92.pdf">Product catalogue HERE</a></blockquote>Thanks to the modular nature of these systems, analysts can easily adapt the configuration to a specific analytical problem, from simple routine analyses to complex characterization of food samples.<h4>Practical Examples of Adulteration Revealed by HPLC</h4><ul><li>Detection of olive oil dilution: HPLC can separate and quantify characteristic triacylglycerols and fatty acids; unusual patterns indicate the presence of cheaper oils.</li><li>Estimation of sugar addition in honey: Isocratic HPLC with a refractive index detector can determine sugar profiles (glucose, fructose, sucrose), revealing dilution or the addition of sugar syrups.</li><li>Identification of aromatic adulterants in wine: Gradient HPLC with a PDA detector can reveal phenolic and aromatic profiles that are altered by the addition of synthetic compounds.</li></ul><h4>Conclusion</h4>Food adulteration remains a current issue that requires reliable and sensitive analytical methods. Liquid chromatography, and in particular modern HPLC systems, represent one of the most powerful tools for detecting unwanted additives, impurities, and unexpected components in complex food samples. Manufacturers such as <a target="_blank" rel="noopener noreferrer" href="https://watrex.com/?utm_source=labrulez&amp;utm_medium=propagace&amp;utm_campaign=labrulez_clanky_HPLCsystemy">Watrex Praha</a> provide flexible chromatographic platforms covering a wide range of analytical tasks from simple isocratic to advanced gradient and GPC/SEC applications thereby supporting analysts in ensuring food quality and consumer protection.<blockquote>Did you find this article interesting? Follow <a target="_blank" rel="noopener noreferrer" href="https://watrex.com/?utm_source=labrulez&amp;utm_medium=propagace&amp;utm_campaign=labrulez_clanky_HPLCsystemy">Watrex Praha</a> on their social media (<a target="_blank" rel="noopener noreferrer" href="https://www.linkedin.com/company/watrex/?viewAsMember=true">LinkedIn</a> and <a target="_blank" rel="noopener noreferrer" href="https://www.facebook.com/WatrexPrague">Facebook</a>) for more engaging topics.</blockquote>

Food Adulteration and How It Is Revealed by Analytical Chemistry

Join us for a fascinating conversation with Dr. Subhoja Chakraborty from the University of Central Florida, whose cutting-edge research targets the global challenge of malaria drug resistance. Dr. Chakraborty shares her journey from structure-based drug design during her PhD to her recent collaborative work repurposing human kinase inhibitors as rapid, selective antimalarials against drug-resistant Plasmodium falciparum strains.<h4>In this episode, you'll learn:</h4><ul><li>How structure-guided approaches reveal potential drug targets, including cysteine proteases like falcipain-2, and kinases implicated in malaria's complex life cycle.</li><li>The power of repurposing kinase inhibitors originally developed for cancer and other diseases, focusing on compound 12, which blocks hemozoin formation and protein kinase 6—key steps in parasite survival.</li><li>Lab techniques such as affinity, gel filtration, and size exclusion chromatography for recombinant protein purification—and how troubleshooting is crucial for high-yield, stable complexes.</li><li>Advances in mass spectrometry for characterizing protein interactions, validating drug targets, and mapping molecular pathways pivotal to malaria therapeutics.</li><li>Tips and lessons for new researchers facing the challenges of sample preparation, complex protocols, and working as part of a collaborative team.</li></ul>Dr. Chakraborty’s insights bridge the gap between basic science and translational drug discovery, offering hope for more effective, targeted malaria treatments in a world where resistance to frontline therapies threatens millions.<h4>Referenced Papers:</h4><ul><li>"Plasmodium falciparum protein kinase 6 and hemozoin formation are inhibited by a type II human kinase inhibitor exhibiting antimalarial activity" (Cell Chemical Biology, 2025)</li><li>"New insights of falcipain 2 structure from Plasmodium falciparum 3D7 strain" (BBRC, 2022)</li><li>"Structure-Based Optimization of Protease−Inhibitor Interactions to Enhance Specificity of Human Stefin‑A against Falcipain‑2 from the Plasmodium falciparum 3D7 Strain" (Biochemistry, 2023)</li></ul>If you’re a student, scientist, or simply passionate about advances in infectious disease research, this episode offers actionable insights, inspiring advice, and a front-row seat to innovation in combating malaria.<h3>Video Transcription</h3><h4>From Structural Biology to Drug Discovery: Chromatography and Mass Spectrometry in Malaria Research</h4><h5>Introduction</h5>In this discussion, Subhoja shares insights into her research journey, focusing on malaria drug discovery and the role of modern analytical techniques in biological and biomedical research. The conversation is aimed primarily at undergraduate students and early-career researchers interested in chemistry, biotechnology, and life sciences.<h5>Research Focus: Malaria Drug Discovery and Kinase Targets</h5>Subhoja is currently involved in malaria drug discovery research at the University of Central Florida, where she has been working since beginning her postdoctoral position two years ago. Her earlier PhD research focused on structure-based drug design in malaria, using a combination of in silico approaches, recombinant protein expression, and X-ray crystallography to determine precise three-dimensional structures of target proteins and their inhibitors.This structural perspective provided a foundation for her current work, which centers on drug discovery through compound screening and repurposing. A recent publication from her laboratory explores the use of repurposed mammalian CDK-like kinase inhibitors as potential antimalarial agents. While such kinase inhibitors are well known in cancer and cardiovascular research, their application to malaria targets represents a novel and promising approach.Kinases are attractive drug targets because of their central role in cell signaling and growth pathways. By targeting specific kinases involved in different stages of the malaria parasite life cycle—whether asexual growth, gametocytogenesis, or differentiation—it may be possible to significantly inhibit parasite growth and progression.<h5>Chromatography in Protein Expression and Purification Workflows</h5>Chromatography plays a critical role throughout Subhoja’s research. Her experience spans from basic chromatography techniques during her early training to advanced protein purification workflows using affinity chromatography, gel filtration (size exclusion chromatography), and ion exchange chromatography.Recombinant proteins are routinely purified using affinity resins such as nickel or cobalt, followed by further purification to remove contaminants. Fast Protein Liquid Chromatography (FPLC) enables compact, stepwise purification workflows suitable for producing proteins for in vitro assays.A recurring challenge in this process is the expression of high-molecular-weight proteins, which often accumulate in insoluble fractions or inclusion bodies. Careful selection of expression vectors, host systems, and induction conditions is therefore essential to maximize yield and solubility.<h5>Integrating Size Exclusion Chromatography into the Workflow</h5>Size exclusion chromatography (SEC) is typically used after affinity purification to further improve protein purity. Following elution with imidazole or glutathione, desalting is required to prevent buffer components from interfering with downstream assays. This is often achieved using desalting columns such as G-25 or PD-10.To remove lower-molecular-weight contaminants, finer SEC media—such as Superdex or Sephacryl columns—are employed. Concentration steps using molecular-weight cut-off filters are then applied to obtain the desired protein concentration for functional studies.These iterative purification and troubleshooting steps are a routine part of protein biochemistry and reflect the importance of methodical optimization and careful validation.<h5>The Role of Mass Spectrometry in Biomolecular Characterization</h5>Mass spectrometry is a key analytical tool in Subhoja’s research pipeline, particularly for target identification and validation. In both malaria and cancer research, MS is used alongside chromatography, immunoprecipitation, and pull-down assays to study protein interactions and molecular recognition events.Affinity-based mass spectrometry enables the identification of binding partners and interactors, whether studying proteins, long non-coding RNAs, or protein complexes. Techniques such as native MS, tandem affinity purification, and proximity labeling (e.g., TurboID-based workflows) provide deeper insights into molecular networks and signaling pathways.These approaches are essential for understanding how candidate drug targets function within complex biological systems.<h5>Sample Preparation Challenges in Biological Research</h5>Sample preparation remains one of the most challenging aspects of biological experimentation. Whole-cell lysates contain proteases, nucleases, and other components that can rapidly degrade target molecules. Preventing degradation requires minimizing preparation time, maintaining low temperatures, and using appropriate inhibitor cocktails.Reproducibility is also critical: biological experiments require multiple replicates, and variability is often unavoidable. Careful handling, protocol optimization, and continuous troubleshooting are essential to ensure reliable results, whether preparing samples for chromatography, mass spectrometry, sequencing, or immunoprecipitation.<h5>Advances in Chromatography and MS for Malaria Research</h5>Modern advances in chromatography and mass spectrometry significantly enhance malaria research by enabling precise target identification, quantification, and functional characterization. Drug discovery workflows begin with construct design and recombinant expression, followed by purification and interaction studies.In malaria research, stage-specific parasite biology requires careful extraction and analysis of proteins from different life cycle stages. Tandem affinity MS, immunoprecipitation, and in vivo validation studies help confirm target relevance and therapeutic potential.Recent work on repurposed kinase inhibitors has shown promising results, including inhibition of hemozoin formation—a key detoxification pathway for the malaria parasite—highlighting the potential of such compounds for further development.<h5>Malaria, Drug Resistance, and Global Impact</h5>Malaria remains a major global health burden, with over 600,000 deaths annually and widespread drug resistance complicating treatment. Artemisinin resistance, particularly associated with mutations in the K13 propeller domain, has driven the search for new targets and therapies.During her PhD, Subhoja focused on falcipain-2, a cysteine protease involved in hemoglobin degradation within the parasite. This pathway is critical for parasite survival and has emerged as a promising drug target. Her work revealed that falcipain-2 operates within a larger protein complex responsible for hemoglobin processing and amino acid release.Understanding these molecular mechanisms is essential for developing robust, stage-specific antimalarial strategies.<h5>Advice for Early-Career Researchers</h5>For researchers just starting out, Subhoja emphasizes the importance of hands-on experience. Working directly with cells, lysates, and analytical tools is essential for truly understanding experimental systems. Failure is an inherent part of research, and perseverance is key.Motivation, teamwork, careful sample handling, and thorough preparation all contribute to long-term success. Reading extensively, critically evaluating one’s own work, and appreciating incremental progress are vital aspects of a sustainable research career.Every successful experiment—no matter how small—represents meaningful progress and contributes to the broader goals of science and medicine.<h5>Closing Remarks</h5>The discussion highlights how interdisciplinary approaches combining chromatography, mass spectrometry, structural biology, and biochemistry are driving advances in malaria drug discovery. Through careful experimental design, analytical rigor, and collaborative teamwork, researchers can address complex biological challenges and contribute to global health solutions.<blockquote>This text has been automatically transcribed from a video presentation using AI technology. It may contain inaccuracies and is not guaranteed to be 100% correct.</blockquote><blockquote><h4>Concentrating on Chromatography Podcast</h4>Dive into the frontiers of chromatography, mass spectrometry, and sample preparation with host David Oliva. Each episode features candid conversations with leading researchers, industry innovators, and passionate scientists who are shaping the future of analytical chemistry. From decoding PFAS detection challenges to exploring the latest in AI-assisted liquid chromatography, this show uncovers practical workflows, sustainability breakthroughs, and the real-world impact of separation science. Whether you’re a chromatographer, lab professional, or researcher you'll discover inspiring content!You can find Concentrating on Chromatography Podcast in podcast apps:<ul><li><a target="_blank" rel="noopener noreferrer" href="https://open.spotify.com/show/1wZg67rCjoGju4yhqxI3tL">Spotify</a></li><li><a target="_blank" rel="noopener noreferrer" href="https://podcasts.apple.com/tz/podcast/concentrating-on-chromatography/id1788429185">Apple podcast</a></li><li><a target="_blank" rel="noopener noreferrer" href="https://podscan.fm/podcasts/concentrating-on-chromatography">podscan</a></li></ul>and on <a target="_blank" rel="noopener noreferrer" href="https://www.youtube.com/@ChromatographyTalk/videos">YouTube channel</a></blockquote>

Affinity, Gel Filtration, Size Exclusion Chromatography: Repurposing Kinase Inhibitors for Malaria

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The Institute of Organic Chemistry and Biochemistry of the Czech Academy of Sciences (IOCB Prague) is a leading scientific institution in the Czech Republic, recognized internationally. Its primary mission is basic research in the fields of chemical biology and medicinal chemistry, organic and material oriented chemistry, chemistry of natural compounds, biochemistry and molecular biology, physical chemistry, theoretical chemistry, and analytical chemistry. 

Institute of Organic Chemistry and Biochemistry of the Czech Academy of Sciences

Organomation, founded in 1959, designs and manufactures high-quality nitrogen evaporators and extraction systems. Known for innovation and durability, their lab instruments are used globally for efficient sample preparation with strong customer support.

Organomation

WATREX Prague is the distributor and producer of HPLC techniques in the Czech Republic and Slovakia. Main Chromatografic and Related Techniques Supported: LC, GPC, IC, IEC, Automated SPE, LC Columns and special column packings, LC method development, Software development, Laboratory Robots.

Watrex Praha

KNAUER AZURA® Pilot Prep HPLC System

Choose the Pilot Prep LC system if you want to increase your productivity even more. As for the AZURA Lab Prep LC system you can freely build up your system. Flow rates up to 1 000 ml/min and loads up to several grams are possible.

AZURA® Preparative HPLC Customized purificationAZURA® preparative systems are the perfect solution for frequently changing separation tasks – from milligram to kilogram scale. Design your AZURA preparative system to your needs and combine flexibility and reliability.<h5>Customaze your system</h5>AZURA® Prep systems are tailor-made for you. Configure your system from injection to detection and choose between different materials, flow rates, valves, and detectors.<h5>Easy scale up from compact to pilot systems</h5>Due to the flexible design of our devices, you can easily change parts like pump heads or flow cells and integrate all components of the compact into the pilot-scale system.<h5>Stacked injections, peak &amp; solvent recycling</h5>AZURA® Prep systems can be used for special separation modes like peak recycling and stacked injections. We help you to configure your system and choose the best software for you.<h3>Flexibility and performance</h3><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/KNAUER_AZURA_Prep_HPLC_System_90fef641c3.jpg" alt="KNAUER AZURA® Prep HPLC System." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_KNAUER_AZURA_Prep_HPLC_System_90fef641c3.jpg 245w," sizes="100vw" width="1920" height="1059"><h2>AZURA® Pilot Prep HPLC System</h2>Choose the Pilot Prep LC system if you want to increase your productivity even more. As for the AZURA Lab Prep LC system you can freely build up your system. Flow rates up to 1 000 ml/min and loads up to several grams are possible. Optional peak and solvent recycling can be set up to increase separation power and reduce separation costs significantly<img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/KNAUER_AZURA_Pilot_Prep_HPLC_System_5_f41d299a7c.jpg" alt="KNAUER: AZURA® Pilot Prep HPLC System." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_KNAUER_AZURA_Pilot_Prep_HPLC_System_5_f41d299a7c.jpg 245w," sizes="100vw" width="1492" height="597"><ul><li data-start="1517" data-end="1580">Pilot Prep HPLC system with binary high-pressure gradient</li><li data-start="1581" data-end="1630">Binary or ternary HPG/LPG, isocratic flow modes</li><li data-start="1631" data-end="1661">Flow rates up to 1000 ml/min</li><li data-start="1662" data-end="1695">20-150 mm recommended column ID</li><li data-start="1696" data-end="1799">Injection system: Sample pump with injection valve for automated handling of large sample volumes</li><li data-start="1800" data-end="1931">Detection system: Supports UVD (VWD, MWD, DAD), RID, FLD, ELSD, MS with split-free UV detection and various flow cell designs</li><li data-start="1932" data-end="2040">Fraction collection: 12-port fractionation valve for automated collection of small and large fractions</li><li data-start="2041" data-end="2080">Stacked injection for high-throughput</li><li data-start="2081" data-end="2156">Software: Intuitive PurityChrom® for direct control and visualization</li><li data-start="2157" data-end="2259">Regulatory compliance: GMP (21 CFR part 11) compliant software and certificates for wetted parts</li><li data-start="2260" data-end="2381">Efficiency features: Solvent recycling to reduce costs and environmental impact, peak-recycling for high resolution</li><li data-start="2382" data-end="2486">High-throughput applications: Liquid handler enables purification of peptides and oligonucleotides</li></ul><h3>Improved purity by combining online SPE with preparative LC</h3>Steviol glycosides are the main sweetening compounds in Stevia rebaudiana and are often used as natural sugar substitutes. To enable commercial usage, the plant extracts need to be purified. In this work, preparative online SPE (solid phase extraction) with the AZURA Pilot Prep LC was investigated for improving overall purity by reducing matrix contamination.The steviol glycoside rebaudioside A is the main compound of interest as it is the sweetest and least bitter compound of the extract. Often, Stevia products contain a mixture of rebaudioside A and stevioside. Developing a purification method with a high yield of rebaudioside A, minimal stevioside impurities, and high throughput increases the economic output of Stevia production.<img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/KNAUER_AZURA_Pilot_Prep_HPLC_System_87addb89e7.jpg" alt="KNAUER: AZURA® Pilot Prep HPLC System." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_KNAUER_AZURA_Pilot_Prep_HPLC_System_87addb89e7.jpg 159w," sizes="100vw" width="1689" height="1656"><h3>Results</h3>Fig. 1 shows the batch LC without online SPE. The matrix peak (1-5 min) negatively affects the separation abilities.&nbsp;<img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/KNAUER_AZURA_Pilot_Prep_HPLC_System_2_625c29b0f1.jpg" alt="KNAUER: AZURA® Pilot Prep HPLC System : Overload experiments on preparative column, 200 μL (red), 500 μL (blue), 2000μL (green); 1) rebaudioside A, 2) stevioside, blue bars – matrix, 25°C, 22 ml/min." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_KNAUER_AZURA_Pilot_Prep_HPLC_System_2_625c29b0f1.jpg 243w," sizes="100vw" width="893" height="573">In comparison, Fig. 2 shows that the automated SPE process significantly decreased the matrix. The fraction analysis revealed that only a small part of the overlapping peak contained nearly pure rebaudioside A; fractions 3-5, approximately 15 mL, had &gt;90% rebaudioside A and &lt;10% stevioside (Fig. 3B).&nbsp;<img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/KNAUER_AZURA_Pilot_Prep_HPLC_System_3_3aa3c283b2.jpg" alt="KNAUER: AZURA® Pilot Prep HPLC System: Preparative online SPE, 10 mL loading; 1) rebaudioside A, 2) stevioside, blue bars – matrix, 25°C, 22 mL/min." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_KNAUER_AZURA_Pilot_Prep_HPLC_System_3_3aa3c283b2.jpg 243w," sizes="100vw" width="867" height="557">The later fractions contained high amounts of stevioside but also still rebaudioside A (Fig. 3C). The results showed that purification of highly pure rebaudioside A is possible with an additional online SPE.<img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/KNAUER_AZURA_Pilot_Prep_HPLC_System_4_e0816cbf59.jpg" alt="KNAUER: AZURA® Pilot Prep HPLC System: Fraction analysis of preparative online-SPE purification (Fig.2) of rebaudioside A (1) and stevioside (2); a) F3 (blue), F4 (red), F5 (green), F6 (light blue); b) F7 (red dashed), F10 (blue dashed), F12 (green sashed), F15 (light blue dashed); c) frac- tionation of target peak, 5 mL fractions." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_KNAUER_AZURA_Pilot_Prep_HPLC_System_4_e0816cbf59.jpg 245w," sizes="100vw" width="916" height="550">

KNAUER AZURA® Bio Compact AC FPLC System

The AZURA Compact AC system qualifies for fast and reliable affinity chromatography. 

KNAUER protein purification: The flexible FPLC platformAZURA® Bio purification systems&nbsp;complete solutions for FPLC on a minimum footprint: AZURA FPLC systems combine flexibility and reliability. The biocompatible/metal-free FPLC is the perfect choice for your protein purification task.<img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/KNAUER_AZURA_Bio_Compact_AC_FPLC_System_6_a874551078.jpg" alt="KNAUER: AZURA® Bio Compact AC FPLC System." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_KNAUER_AZURA_Bio_Compact_AC_FPLC_System_6_a874551078.jpg 245w," sizes="100vw" width="1506" height="600">Design your AZURA Bio purification system to fit your needs. Multiple functionalities such as automatic sample injection via autosampler, column switching, buffer and sample selection, and fraction collection enable automation of the purification process.A wide range of detectors ensures the visibility of target molecules. Various flow rates and compatibility with columns from all vendors provide maximum flexibility. The intuitive PurityChrom® software integrates all the advantages of a versatile purification system.<h3>AZURA® Bio Compact AC FPLC System</h3><h4>For affinity chromatography</h4>The AZURA Compact AC system qualifies for fast and reliable affinity chromatography. Select your sample, washing, and elution buffer using the selection valve. Your proteins of interest are detected by UV and automatically collected via the fractionation valve.<img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/KNAUER_AZURA_Bio_Compact_AC_FPLC_System_4_bef2309e7d.jpg" alt="KNAUER: AZURA® Bio Compact AC FPLC System." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_KNAUER_AZURA_Bio_Compact_AC_FPLC_System_4_bef2309e7d.jpg 245w," sizes="100vw" width="1666" height="513"><h3>Key features</h3><ul><li data-start="93" data-end="153">Flow rate: 0.01–50 ml/min (recommended: 1–40 ml/min)</li><li data-start="154" data-end="193">Maximum system pressure: 50 bar</li><li data-start="194" data-end="278">Sample and buffer selection: Automatic valve for up to 12 buffers or samples</li><li data-start="279" data-end="349">Detection: Variable single wavelength UV detector (190–500 nm)</li><li data-start="350" data-end="429">Column compatibility: Compatible with affinity columns from all vendors</li><li data-start="430" data-end="574">Fraction collection: 16-port fractionation valve, capable of collecting up to 16 fractions for flow-through, wash, and elution fractions</li><li data-start="575" data-end="663">Software: Intuitive and powerful PurityChrom® software, 21 CFR Part 11 compliant</li><li data-start="664" data-end="737">Affinity purification: Designed for tagged proteins or antibodies</li><li data-start="738" data-end="867">Compact system: User-friendly and cost-efficient, with the ability to upgrade for more complex protein purification tasks</li></ul><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/KNAUER_AZURA_Bio_Compact_AC_FPLC_System_5_17f4f13731.jpg" alt="KNAUER AZURA® Bio Compact AC FPLC System: Protein purification based on high affinity Chromatogram &amp; Legend." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_KNAUER_AZURA_Bio_Compact_AC_FPLC_System_5_17f4f13731.jpg 209w," sizes="100vw" width="1024" height="766">&nbsp;

KNAUER AZURA® Bio Compact SEC FPLC System

AZURA Compact SEC systems handle time-consuming SEC methods in your lab without occupying your valuable FPLC system.

KNAUER protein purification: The flexible FPLC platformAZURA® Bio purification systems&nbsp;complete solutions for FPLC on a minimum footprint: AZURA FPLC systems combine flexibility and reliability. The biocompatible/metal-free FPLC is the perfect choice for your protein purification task.<img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/KNAUER_AZURA_Bio_Compact_AC_FPLC_System_6_a874551078.jpg" alt="KNAUER: AZURA® Bio Compact AC FPLC System." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_KNAUER_AZURA_Bio_Compact_AC_FPLC_System_6_a874551078.jpg 245w," sizes="100vw" width="1506" height="600">Design your AZURA Bio purification system to fit your needs. Multiple functionalities such as automatic sample injection via autosampler, column switching, buffer and sample selection, and fraction collection enable automation of the purification process.A wide range of detectors ensures the visibility of target molecules. Various flow rates and compatibility with columns from all vendors provide maximum flexibility. The intuitive PurityChrom® software integrates all the advantages of a versatile purification system.<h3>AZURA® Bio Compact SEC FPLC System</h3><h4>For Size Exclusion Chromatography (SEC)</h4>AZURA Compact SEC systems take over time-consuming SEC methods in your lab without blocking your valuable FPLC system. Due to its compact design and intuitive FPLC software PurityChrom®, the system offers outstanding performance and ease of use.&nbsp;Pre-designed methods are included in the software for the designated SEC system and can be easily adapted by changing the column volume. AZURA Compact SEC supports all columns available on the market.<img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/KNAUER_AZURA_Bio_Compact_SEC_FPLC_System_4_f5efb08061.jpg" alt="KNAUER: AZURA® Bio Compact SEC FPLC System " srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_KNAUER_AZURA_Bio_Compact_SEC_FPLC_System_4_f5efb08061.jpg 245w," sizes="100vw" width="1358" height="540"><h3>Key features</h3><ul><li data-start="93" data-end="157">Flow rate: 0.001–10 ml/min (recommended: 0.1–8.0 ml/min)</li><li data-start="158" data-end="197">Maximum system pressure: 50 bar</li><li data-start="198" data-end="276">Injection system: Injection valve for sample injection via sample loop</li><li data-start="277" data-end="347">Detection: Variable single wavelength UV detector (190–500 nm)</li><li data-start="348" data-end="423">Fraction collection: Fraction collector for automated fractionation</li><li data-start="424" data-end="522">Column compatibility: Supports columns from all vendors, including third-party SEC columns</li><li data-start="523" data-end="611">Software: Intuitive and powerful PurityChrom® software, 21 CFR Part 11 compliant</li><li data-start="612" data-end="780">System design:<ul data-start="637" data-end="780"><li data-start="637" data-end="666">Isocratic buffer delivery</li><li data-start="669" data-end="687">Loop injection</li><li data-start="690" data-end="734">Wide range of collection racks available</li><li data-start="737" data-end="780">Specially designed for SEC applications</li></ul></li></ul><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/KNAUER_AZURA_Bio_Compact_SEC_FPLC_System_3_7a4bc1f60b.jpg" alt="KNAUER: AZURA® Bio Compact SEC FPLC System " srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_KNAUER_AZURA_Bio_Compact_SEC_FPLC_System_3_7a4bc1f60b.jpg 245w," sizes="100vw" width="1920" height="1152">Don't use your valuable gradient FPLC system for boring and time-consuming size exclusion separations. Choose your dedicated AZURA SEC system.<figure class="image"><img style="aspect-ratio:690/490;" src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/KNAUER_AZURA_Bio_Compact_SEC_FPLC_System_45d5079064.webp" alt="KNAUER: AZURA® Bio Compact SEC FPLC System." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_KNAUER_AZURA_Bio_Compact_SEC_FPLC_System_45d5079064.webp 220w," sizes="100vw" width="690" height="490"></figure>With its compact design and intuitive software, the system offers excellent performance, ease of use and supports all SEC columns available on the market.​

KNAUER AZURA® Bio Lab FPLC System

AZURA Bio Lab allows you to create FPLC systems with highest independence. Just pick your modules and build-up the system yourself. The intuitive software PurityChrom will be flexibly adjusted to your system.

KNAUER protein purification: The flexible FPLC platformAZURA® Bio purification systems&nbsp;complete solutions for FPLC on a minimum footprint: AZURA FPLC systems combine flexibility and reliability. The biocompatible/metal-free FPLC is the perfect choice for your protein purification task.<img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/KNAUER_AZURA_Bio_Compact_AC_FPLC_System_6_a874551078.jpg" alt="KNAUER: AZURA® Bio Compact AC FPLC System." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_KNAUER_AZURA_Bio_Compact_AC_FPLC_System_6_a874551078.jpg 245w," sizes="100vw" width="1506" height="600">Design your AZURA Bio purification system to fit your needs. Multiple functionalities such as automatic sample injection via autosampler, column switching, buffer and sample selection, and fraction collection enable automation of the purification process.A wide range of detectors ensures the visibility of target molecules. Various flow rates and compatibility with columns from all vendors provide maximum flexibility. The intuitive PurityChrom® software integrates all the advantages of a versatile purification system.<h3>AZURA® Bio Lab FPLC System</h3><h4>R&amp;D-scale gradient system for complex purifications of bio molecules</h4>AZURA Bio Lab allows you to create FPLC systems with highest independence. Just pick your modules and build-up the system yourself. The intuitive software PurityChrom will be flexibly adjusted to your system.<img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/KNAUER_AZURA_Bio_Lab_FPLC_System_3_7b22a3e099.jpg" alt="KNAUER: AZURA® Bio Lab FPLC System." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_KNAUER_AZURA_Bio_Lab_FPLC_System_3_7b22a3e099.jpg 245w," sizes="100vw" width="1645" height="671"><h3>Key features</h3><ul><li data-start="93" data-end="145">Flow rate: 0.001–50 ml/min (maximum 240 bar)</li><li data-start="146" data-end="224">Injection system: Sample injections via sample loop and/or sample pump</li><li data-start="225" data-end="295">Detection: Variable single wavelength UV detector (190–500 nm)</li><li data-start="296" data-end="386">Fraction collection: XY fraction collector for large volumes or numerous fractions</li><li data-start="387" data-end="493">Column compatibility: Supports up to 7 columns for method development – all FPLC columns supported</li><li data-start="494" data-end="584">Suitable for Ion-exchange, HILIC, affinity, SEC, and RP</li><li data-start="585" data-end="640">Reproducible and automated protein purification</li><li data-start="641" data-end="695">System usability: Suitable for cold room usage</li><li data-start="696" data-end="820">Software:<ul data-start="716" data-end="820"><li data-start="716" data-end="780">Intuitive and powerful PurityChrom® software (basic license)</li><li data-start="783" data-end="820">21 CFR Part 11 compliant software</li></ul></li><li data-start="821" data-end="924">Additional features:<ul data-start="852" data-end="924"><li data-start="852" data-end="924">Conductivity and pH-monitoring, pressure, and air sensors (optional)</li></ul></li></ul><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/KNAUER_AZURA_Bio_Lab_FPLC_System_4740938659.jpg" alt="KNAUER: AZURA® Bio Lab FPLC System." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_KNAUER_AZURA_Bio_Lab_FPLC_System_4740938659.jpg 235w," sizes="100vw" width="1920" height="1279">From simple to advanced, from lab to process scale – configure your FPLC with our modular design according to your purification needs.Designed and optimized for all FPLC applications such as affinity, ion exchange, hydrophobic interaction and size exclusion chromatography. All system modules can be expanded with additional modules.

KNAUER AZURA® Bio Lab Advanced FPLC System

Gradient-capable FPLC system for a wide range of purifications including automated two-step applications

KNAUER protein purification: The flexible FPLC platformAZURA® Bio purification systems&nbsp;complete solutions for FPLC on a minimum footprint: AZURA FPLC systems combine flexibility and reliability. The biocompatible/metal-free FPLC is the perfect choice for your protein purification task.<img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/KNAUER_AZURA_Bio_Compact_AC_FPLC_System_6_a874551078.jpg" alt="KNAUER: AZURA® Bio Compact AC FPLC System." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_KNAUER_AZURA_Bio_Compact_AC_FPLC_System_6_a874551078.jpg 245w," sizes="100vw" width="1506" height="600">Design your AZURA Bio purification system to fit your needs. Multiple functionalities such as automatic sample injection via autosampler, column switching, buffer and sample selection, and fraction collection enable automation of the purification process.A wide range of detectors ensures the visibility of target molecules. Various flow rates and compatibility with columns from all vendors provide maximum flexibility. The intuitive PurityChrom® software integrates all the advantages of a versatile purification system.<h3>AZURA® Bio Lab Advanced FPLC System</h3><h4>Gradient-capable FPLC system for a wide range of purifications including automated two-step applications</h4>Protein purification involves most of the times two to three steps:<ol><li>capture step</li><li>optional intermediated step</li><li>polishing step</li></ol>The transition from one to another step generally involves manual interaction and thus is time-consuming. Automation by combining these steps increases the efficiency and optimizes the workflow.&nbsp;The quick and automated linkage of multiple chromatographic purification steps into one method eliminates manual sample handling and minimizes time spent between steps. This automation strategy can be easily adapted to each purification task.<img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/KNAUER_AZURA_Bio_Lab_FPLC_System_3_7b22a3e099.jpg" alt="KNAUER: AZURA® Bio Lab Advanced FPLC System." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_KNAUER_AZURA_Bio_Lab_FPLC_System_3_7b22a3e099.jpg 245w," sizes="100vw" width="1645" height="671"><h3>Key features</h3><ul data-start="93" data-end="1077"><li data-start="93" data-end="144">Flow rate: 0.001–50 ml/min (maximum 50 bar)</li><li data-start="145" data-end="263">Injection system: Sample injection via loop or sample pump, with automated storage and reinjection of proteins</li><li data-start="264" data-end="433">Detection system:<ul data-start="292" data-end="433"><li data-start="292" data-end="320">Multichannel UV detector</li><li data-start="323" data-end="405">Highly sensitive multi-wavelength detector for proteins, DNA/RNA, and peptides</li><li data-start="408" data-end="433">Conductivity detector</li></ul></li><li data-start="434" data-end="566">Fraction collection: XY fraction collector with outlet valve for automated collection of large volumes or numerous fractions</li><li data-start="567" data-end="673">Column compatibility: Supports up to 7 columns for method development – all FPLC columns supported</li><li data-start="674" data-end="764">Suitable for Ion-exchange, HILIC, affinity, SEC, and RP</li><li data-start="765" data-end="864">Automation: Enables challenging protein purification tasks with minimal manual intervention</li><li data-start="865" data-end="973">Software:<ul data-start="885" data-end="973"><li data-start="885" data-end="933">Intuitive and powerful PurityChrom® software</li><li data-start="936" data-end="973">21 CFR Part 11 compliant software</li></ul></li><li data-start="974" data-end="1077">Additional features:<ul data-start="1005" data-end="1077"><li data-start="1005" data-end="1077">Conductivity and pH-monitoring, pressure, and air sensors (optional)</li></ul></li></ul><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/KNAUER_AZURA_Bio_Lab_Advanced_FPLC_System_3_9cf517ceaf.webp" alt="KNAUER: AZURA® Bio Lab Advanced FPLC System." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_KNAUER_AZURA_Bio_Lab_Advanced_FPLC_System_3_9cf517ceaf.webp 234w," sizes="100vw" width="690" height="460"><h3>Two-step purification: Special multi-column chromatography solutions</h3>Designed and optimized for all FPLC applications such as affinity, ion exchange, hydrophobic interaction and size exclusion chromatography. In addition, you can perform dedicated twoSTEP applications.<img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/KNAUER_AZURA_Bio_Lab_Advanced_FPLC_System_44bff80cfc.webp" alt="KNAUER: AZURA® Bio Lab Advanced FPLC System." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_KNAUER_AZURA_Bio_Lab_Advanced_FPLC_System_44bff80cfc.webp 209w," sizes="100vw" width="1920" height="1434"><h3>Automated two-step purification of mouse IgG antibodies</h3><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/KNAUER_AZURA_Bio_Lab_Advanced_FPLC_System_4_c86d2177ce.jpg" alt="KNAUER: AZURA® Bio Lab Advanced FPLC System: The affinity chromatography step was automatically combined with a gel filtration step to exchange the buffer of the purified mouse IgG antibodies; Phase 1: Column equilibration, Phase 2: Sample injection and washing, Phase 3: Elution of IgG from protein A column, Phase 4: Desalting of IgG." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_KNAUER_AZURA_Bio_Lab_Advanced_FPLC_System_4_c86d2177ce.jpg 245w," sizes="100vw" width="1085" height="605">

KNAUER AZURA® Bio Lab Pilot FPLC System

Configure your FPLC system based on your purification requirements. The Pilot system is designed for scale-up and process applications. All system modules can be expanded with additional modules.

KNAUER protein purification: The flexible FPLC platformAZURA® Bio purification systems&nbsp;complete solutions for FPLC on a minimum footprint: AZURA FPLC systems combine flexibility and reliability. The biocompatible/metal-free FPLC is the perfect choice for your protein purification task.<img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/KNAUER_AZURA_Bio_Compact_AC_FPLC_System_6_a874551078.jpg" alt="KNAUER: AZURA® Bio Compact AC FPLC System." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_KNAUER_AZURA_Bio_Compact_AC_FPLC_System_6_a874551078.jpg 245w," sizes="100vw" width="1506" height="600">Design your AZURA Bio purification system to fit your needs. Multiple functionalities such as automatic sample injection via autosampler, column switching, buffer and sample selection, and fraction collection enable automation of the purification process.A wide range of detectors ensures the visibility of target molecules. Various flow rates and compatibility with columns from all vendors provide maximum flexibility. The intuitive PurityChrom® software integrates all the advantages of a versatile purification system.<h3>AZURA® Bio Lab Pilot FPLC System</h3><h4>High Flow Bio Purification FPLC System – up to 1000 ml/min</h4>Configure your FPLC system based on your purification requirements. The Pilot system is designed for scale-up and process applications. All system modules can be expanded with additional modules.<img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/KNAUER_AZURA_Bio_Lab_Pilot_FPLC_System_e208baf41d.jpg" alt="KNAUER: AZURA® Bio Lab Pilot FPLC System." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_KNAUER_AZURA_Bio_Lab_Pilot_FPLC_System_e208baf41d.jpg 156w," sizes="100vw" width="1000" height="1000"><h3>Key features</h3><ul data-start="93" data-end="1069"><li data-start="93" data-end="129">Flow rate: 0.001–1000 ml/min</li><li data-start="130" data-end="192">Injection system: Sample pump for large sample volumes</li><li data-start="193" data-end="351">Detection system:<ul data-start="221" data-end="351"><li data-start="221" data-end="276">Variable single wavelength UV detector (190–500 nm)</li><li data-start="279" data-end="351">Conductivity and pH-monitoring, pressure, and air sensors (optional)</li></ul></li><li data-start="352" data-end="465">Fraction collection: XY fraction collector for reliable collection of large volumes or numerous fractions</li><li data-start="466" data-end="559">Suitable for SEC, AC, IEX, and HIC, with upscaling capabilities</li><li data-start="560" data-end="651">Automation: High-flow buffer delivery and mixing for large-scale purification tasks</li><li data-start="652" data-end="739">System design: Same footprint as <a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/products/1625">AZURA Lab FPLC systems</a> for compact scalability</li><li data-start="740" data-end="871">Software:<ul data-start="760" data-end="871"><li data-start="760" data-end="831">Intuitive and powerful PurityChrom® software in basic configuration</li><li data-start="834" data-end="871">21 CFR Part 11 compliant software</li></ul></li><li data-start="872" data-end="1069">Scalability:<ul data-start="895" data-end="1069"><li data-start="895" data-end="965">Configure your FPLC system based on your purification requirements</li><li data-start="968" data-end="1069">Scale-up is possible with the same flexibility and software while maintaining a minimal footprint</li></ul></li></ul>

KNAUER AZURA® SEC Compact System

The AZURA SEC Compact systems provide excellent price-to-performance value, ideal for labs entering GPC/SEC, process control, or those with limited budgets and educational needs.

<h3>Compact, versatile system for SEC and GPC applications&nbsp;</h3>The AZURA SEC Compact systems offer an outstanding price-to-performance ratio and are the perfect solution for every laboratory entering the analytical world of GPC/SEC, for process control applications, or for limited-budget and educational laboratories.The heart of the systems is the Assistant ASM 2.2L is a docking station for three compact devices. Valves, pumps and UV detectors can be combined in one housing. Routine maintenance work e.g. replacing the lamp of a detector is easily performed by the user.Thanks to its modular concept the systems can be extended with additional components at any time later.<img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/KNAUER_AZURA_SEC_Compact_System_3_313d153f48.jpg" alt="KNAUER: AZURA® SEC Compact System." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_KNAUER_AZURA_SEC_Compact_System_3_313d153f48.jpg 237w," sizes="100vw" width="1009" height="664"><h3 data-start="857" data-end="879">Key Features</h3><ul data-start="880" data-end="1168"><li data-start="880" data-end="914">Flow rate: 0.001–10 ml/min</li><li data-start="915" data-end="946">Pressure: Up to 400 bar</li><li data-start="947" data-end="997">Intuitive operation with maximum precision</li><li>Injection via valve drive for minimal pressure spikes</li><li data-start="1043" data-end="1095">Supports a wide range of SEC and GPC columns</li><li data-start="1096" data-end="1128">Dedicated SEC/GPC ClarityChrom® add-on for in-depth data evaluation</li><li data-start="1129" data-end="1168">Expandable, space-saving design</li><li data-start="1129" data-end="1168">Suitable for organic and aqueous eluents</li></ul><h3 data-start="1175" data-end="1219">System Components &amp; Configurations</h3><ul data-start="1220" data-end="1486"><li data-start="1220" data-end="1242">Isocratic pump</li><li data-start="1243" data-end="1270">UV and RI detectors</li><li data-start="1271" data-end="1296">Column thermostat</li><li data-start="1297" data-end="1335">Automatic injection (optional)</li><li data-start="1336" data-end="1393">ClarityChrom® add-on for in-depth data evaluation</li><li data-start="1394" data-end="1486">Choose a hardware version for aqueous SEC of biomolecules or organic GPC of polymers</li></ul><h3 data-start="1493" data-end="1520">Detection Options</h3><ul data-start="1521" data-end="1555"><li data-start="1521" data-end="1555">RI, UV, ELSD, PDA, FLD, MS</li></ul><h3 data-start="1562" data-end="1587">Upgrade Options</h3><ul data-start="1588" data-end="1745"><li data-start="1588" data-end="1649">Automatic solvent recycle valve (cost-saving feature)</li><li data-start="1650" data-end="1666">Degasser</li><li data-start="1667" data-end="1686">Autosampler</li><li data-start="1687" data-end="1745">Additional detectors (RI, UV, ELSD, PDA, FLD, ECD)</li></ul>&nbsp;<figure class="image"><img style="aspect-ratio:1886/1452;" src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/KNAUER_AZURA_SEC_Compact_System_3c56c6482f.webp" alt="KNAUER: AZURA® SEC Compact System." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_KNAUER_AZURA_SEC_Compact_System_3c56c6482f.webp 203w," sizes="100vw" width="1886" height="1452"></figure>

KNAUER AZURA® SEC Lab System

The AZURA SEC Lab systems are ideal for labs requiring top analytical performance, offering unmatched flow precision and ultra-low pulsation for exceptional retention volume accuracy and reproducibility.

<h3>Highest analytical performance and robustness for SEC and GPC applications</h3>The AZURA SEC Lab systems are the perfect choice for laboratories that demands highest analytical performance. The system shows unsurpassed flow precision paired with ultra-low pulsation for outstanding retention volume precision and reproducibility.The autosampler can inject from up to 768 positions via microtiter plates or 108 standard 1.5 ml sample vials and is therefore the perfect choice for larger sample amount as well as for high sample throughput laboratories. Thanks to the reliable and robust design it is the workhorse in any laboratory.<img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/KNAUER_AZURA_SEC_Lab_System_e161ecef84.webp" alt="KNAUER: AZURA® SEC Lab System." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_KNAUER_AZURA_SEC_Lab_System_e161ecef84.webp 203w," sizes="100vw" width="1886" height="1452"><h3>Key Features</h3><ul><li>Injection volume: 1 –1000 µl&nbsp;</li><li>Easy maintenance</li><li>Great selection of SEC/GPC columns</li><li>Unsurpassed flow precision paired with ultra-low pulsation for</li><li>Highest analytical performance</li><li>Suitable for organic and aqueous eluents</li><li>Highest level of automation</li><li>Choose the matching hardware version for aqueous SEC of biomolecules or organic GPC of polymers</li></ul><h3>Upgrade Options</h3><ul><li>Save operating costs with an automatic solvent recycle valve</li><li>Degasser</li><li>Detector (RI, UV, ELSD, PDA, FLD, ECD)</li></ul><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/KNAUER_AZURA_SEC_Lab_System_2_2cbe13a95d.webp" alt="KNAUER: AZURA® SEC Lab System." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_KNAUER_AZURA_SEC_Lab_System_2_2cbe13a95d.webp 234w," sizes="100vw" width="953" height="635"> All wetted parts are available in either bioinert materials or for standard phase solvents.

KNAUER AZURA® SEC Semi-Prep System

The AZURA SEC Semi-prep systems enable fractionation of specific molecular weight ranges or purification tasks, with a pump delivering up to 50 ml/min, making it ideal for all semi-preparative GPC/SEC columns.

<h3>Automated system for semi-preparative SEC and GPC</h3>The AZURA SEC Semi-prep systems offer the possibility of fractionating specific molecular weight ranges or purification tasks. The pump can deliver flow rates of up to 50 ml/min and is therefore suitable for every semi-preparative GPC/SEC column.Thanks to the modular concept of the AZURA platform and the dedicated PurityChrom® software, the systems can be highly adapted to any separation task.<img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/KNAUER_AZURA_SEC_Semi_Prep_System_12215b2eda.webp" alt="KNAUER: AZURA® SEC Semi-Prep System." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_KNAUER_AZURA_SEC_Semi_Prep_System_12215b2eda.webp 203w," sizes="100vw" width="1886" height="1452"><h3>Fraction Collection</h3>Collect large quantities or large numbers of fractions:<ul style="list-style-type:disc;"><li style="tab-stops:list 36.0pt;">Manually – collection by direct control</li><li style="tab-stops:list 36.0pt;">Volume-based – collection at defined volumes</li><li style="tab-stops:list 36.0pt;">Peak-based – collection according to detector signal</li><li style="tab-stops:list 36.0pt;">From switching valve to fraction collector solutions</li></ul><h3>Key Features</h3><ul><li data-start="70" data-end="134">Modular concept for any separation and purification task</li><li data-start="135" data-end="169">Flow rates: 0.01–50 ml/min</li><li data-start="170" data-end="249">Injection options: Manual or autosampler (up to 10 ml injection volume)</li><li data-start="250" data-end="320">Eluent compatibility: Suitable for organic and aqueous eluents</li><li data-start="321" data-end="390">Column selection: Wide range of SEC and GPC columns available</li><li data-start="563" data-end="649">Efficiency features: Automatic solvent recycle valve to reduce operating costs</li><li data-start="650" data-end="757">Hardware options: Choose a version for aqueous SEC (biomolecules) or organic GPC (polymers)</li><li data-start="758" data-end="813">Software options: ClarityChrom® or PurityChrom®</li></ul><h3>Available Detectors for Semi-prep</h3>The AZURA SEC Semi-prep systems can be equipped with a dedicated high-flow RI detector that can be used up to 10 ml/min as well as with the UV Detectors UVD 2.1S and UVD 2.1L.

KNAUER AZURA® GPC Cleanup System

Do not waste time and labor with manual sample preparation. The AZURA GPC Cleanup system automates work-intensive and time-consuming cleanup tasks based on gel permeation chromatography (GPC).

<h3>Save time with automatic sample preparation</h3>Do not waste time and labor with manual sample preparation. The AZURA GPC Cleanup system automates work-intensive and time-consuming cleanup tasks based on gel permeation chromatography (GPC). Further, reproducibility and quality of the cleanup are improved.After sample loading, the dedicated system fully automated processes up to 15 samples while requiring minimal bench space. Based on the versatile AZURA device platform the system can be customized to fulfill individual requirements. The cost-effective software Mobile Control provides a touch-optimized user interface for intuitive device control directly at the system.<img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/KNAUER_AZURA_GPC_Cleanup_System_224a241335.webp" alt="KNAUER: AZURA® GPC Cleanup System." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_KNAUER_AZURA_GPC_Cleanup_System_224a241335.webp 203w," sizes="100vw" width="1886" height="1452"><h3>Key Features</h3><ul><li data-start="69" data-end="102">Flow rate: 0.01–10 ml/min</li><li data-start="103" data-end="169">Enhanced reproducibility – consistent and reliable results</li><li data-start="170" data-end="214">Handles viscous and complex matrices</li><li data-start="215" data-end="269">Fully automated processing of up to 15 samples</li><li data-start="270" data-end="320">Supports AOAC, EPA, and European Standards</li><li data-start="321" data-end="365">Adaptable to specific lab procedures</li><li data-start="366" data-end="405">Compact, space-efficient system</li><li data-start="406" data-end="466">Touch-optimized Mobile Control for device management</li><li data-start="467" data-end="551">Automated workflows for streamlined sample preparation and higher efficiency</li><li data-start="552" data-end="606">Optimized cleanup tasks for better performance</li><li data-start="607" data-end="658">Easily adaptable for different applications</li></ul><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/KNAUER_AZURA_GPC_Cleanup_System_2_27867ae999.jpg" alt="KNAUER: AZURA® GPC Cleanup System: Chromatogram of US EPA method 3640A calibration standard containing 1) Corn oil matrix 2) Bis-(2-ethylhexyl)phalate 3) Methoxychlor 4) Perylene." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_KNAUER_AZURA_GPC_Cleanup_System_2_27867ae999.jpg 245w," sizes="100vw" width="1247" height="626">In accordance with established methods to determine pesticide residues<ul><li>Method 984.21 – AOAC international</li><li>EPA SW-846 Method 3640A – US Environmental Protection Agency</li><li>AEN 12393 and EN 1528 – European Standard</li><li>L 00.00-34 Method in accordance with §64 LFGB (formerly § 35 LMBG)</li></ul>

Products from category Liquid chromatographs from KNAUER - page 1

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