<p><strong>🎤Dr. Christopher Reid (Professor of Chemistry and Biochemistry at Bryant University in Rhode Island)</strong></p><p><strong>In this episode of Concentrating on Chromatography, host Dave Oliva sits down with Chris Reid to explore how chemical biology and LC-MS are being used to tackle one of the most urgent global challenges: antimicrobial resistance.</strong></p><p>As antibiotic resistance continues to rise—and new drug development struggles to keep pace—researchers are looking beyond traditional approaches. Instead of targeting how bacteria build their cell walls, Chris’s work focuses on how they break them down, opening the door to entirely new antimicrobial strategies.</p><h4><strong>What You’ll Learn in This Episode:</strong></h4><ul><li>Why antimicrobial resistance is accelerating globally</li><li>What peptidoglycan is and why it’s essential to bacterial survival</li><li>How targeting cell wall degradation differs from traditional antibiotics</li><li>The strategy behind designing small molecule probes and inhibitors</li><li>How LC-MS is used to analyze peptidoglycan fragments and enzyme activity</li><li>Key analytical challenges: complex mixtures, low-abundance species, and structural similarity</li><li>The importance of sample preparation and reproducibility in LC-MS workflows</li><li>How this research could lead to new antibiotic targets</li></ul><h3><strong>Video Transcription</strong></h3><h4><strong>The Growing Challenge of Antibiotic Resistance</strong></h4><p>According to Reid, antibiotic resistance is increasing across a broad spectrum of bacterial pathogens that were once readily treated with existing therapies. In some cases, clinicians must now rely on only a handful of last-line antibiotics, while resistance is also rising in diseases such as tuberculosis.</p><p>One of the major obstacles is the limited commercial incentive to develop entirely new antibiotics. Because bacterial resistance can emerge relatively quickly after a drug reaches the market, pharmaceutical companies often favor modifying existing antibiotics or combining them with resistance-modifying agents rather than investing in completely new drug classes. As a result, genuinely novel antibacterial targets remain urgently needed.</p><h4><strong>Why the Bacterial Cell Wall Remains an Attractive Target</strong></h4><p>The bacterial cell wall consists primarily of <strong>peptidoglycan</strong>, a highly cross-linked polymer that provides structural integrity, maintains cell shape, and enables bacterial growth and division.</p><p>Many widely used antibiotics—including the β-lactam antibiotics such as penicillins—target enzymes responsible for synthesizing or cross-linking peptidoglycan. These pathways have proven highly successful therapeutic targets because peptidoglycan is unique to bacteria and absent from human cells, minimizing unwanted effects on the host.</p><p>However, most existing antibiotics target only specific stages of peptidoglycan biosynthesis. Reid's research explores another part of this complex pathway: enzymes responsible for degrading the carbohydrate backbone of peptidoglycan during normal bacterial growth and remodeling. These enzymes may represent an underexplored class of antimicrobial targets capable of overcoming existing resistance mechanisms.</p><h4><strong>Targeting the Glycan Backbone</strong></h4><p>A key advantage of focusing on the carbohydrate backbone is its remarkable conservation across both Gram-positive and Gram-negative bacteria.</p><p>Whereas the peptide components of peptidoglycan vary considerably between bacterial species—and frequently acquire mutations associated with antibiotic resistance—the glycan backbone remains highly conserved and tolerates relatively few structural modifications.</p><p>This biological constraint makes enzymes acting on the glycan backbone particularly attractive targets. Reid's group developed a series of <strong>small-molecule probes and inhibitors</strong> designed to recognize enzymes known as <strong>N-acetylglucosaminidases</strong>, which cleave glycosidic bonds within peptidoglycan and generate soluble degradation products.</p><p>Rather than beginning with purified enzymes, the researchers adopted a <strong>top-down phenotypic screening strategy</strong>. Candidate compounds were first evaluated for their ability to inhibit bacterial growth before progressively narrowing the list of potential molecular targets through genetic studies, microscopy, enzyme assays, and biochemical characterization. This iterative workflow ultimately identified compounds capable of inhibiting the desired enzyme class while simultaneously providing valuable chemical probes for studying bacterial cell wall biology.</p><h4><strong>LC-MS as a Central Analytical Tool</strong></h4><p>Liquid chromatography coupled with mass spectrometry plays a central role throughout the project by enabling detailed characterization of structural changes occurring within peptidoglycan after bacterial cells are treated with candidate inhibitors.</p><p>Because intact peptidoglycan is an insoluble polymer, sample preparation begins with isolation of the bacterial cell wall through detergent extraction and differential centrifugation. Additional purification steps remove proteins, nucleic acids, and teichoic acids before enzymatic digestion converts the insoluble material into soluble fragments suitable for chromatographic analysis.</p><p>The resulting digest is analyzed by LC-MS to examine changes in the distribution of peptidoglycan fragments. Chromatographic retention times provide an immediate indication of changes in cross-linking, with larger oligomers such as trimers and tetramers generally eluting later than monomeric fragments. Mass spectrometry then confirms the identity of these fragments by measuring their intact molecular masses, while tandem MS experiments can provide further structural information when required.</p><h4><strong>Sample Preparation Determines Data Quality</strong></h4><p>Reid emphasized that sample preparation represents one of the most critical—and challenging—stages of the entire workflow.</p><p>Because peptidoglycan analysis involves multiple purification, digestion, and reduction steps, even minor procedural differences between samples can introduce analytical artifacts that complicate biological interpretation.</p><p>Maintaining strict consistency throughout sample preparation is therefore essential. Appropriate controls must accompany every experiment, ensuring that observed chromatographic and mass spectrometric differences truly reflect biological responses rather than experimental variability. The complexity of bacterial cell wall composition further increases the analytical challenge, as subtle differences in amino acid substitutions and low-abundance cross-linked species can complicate LC-MS data interpretation.</p><h4><strong>Chemical Biology for Future Antibiotic Discovery</strong></h4><p>Although the identified compounds may eventually serve as starting points for antibiotic development, Reid views them primarily as <strong>chemical biology tools</strong>.</p><p>By selectively inhibiting individual enzymes, these probes allow researchers to investigate previously unexplored aspects of bacterial cell wall remodeling that cannot easily be studied using classical genetic approaches alone.</p><p>The research team is now expanding this strategy by combining their probes with <strong>proximity-labeling proteomics and mass spectrometry</strong>, aiming to map protein interaction networks associated with bacterial cell wall degradation. Such studies may reveal entirely new antibacterial targets and provide deeper insight into the biological processes governing bacterial growth and division.</p><p>Ultimately, the work illustrates how integrating synthetic chemistry, microbiology, molecular biology, and advanced LC-MS can accelerate the search for innovative therapeutic strategies against antimicrobial resistance. As existing antibiotics become progressively less effective, multidisciplinary approaches such as these may play an increasingly important role in identifying the next generation of antibacterial targets.</p><blockquote><p><strong>This text has been automatically transcribed from a video presentation using AI technology. It may contain inaccuracies and is not guaranteed to be 100% correct.</strong></p></blockquote><blockquote><h4><strong>Concentrating on Chromatography Podcast</strong></h4><p>Dive into the frontiers of chromatography, mass spectrometry, and sample preparation with host David Oliva. Each episode features candid conversations with leading researchers, industry innovators, and passionate scientists who are shaping the future of analytical chemistry. From decoding PFAS detection challenges to exploring the latest in AI-assisted liquid chromatography, this show uncovers practical workflows, sustainability breakthroughs, and the real-world impact of separation science. Whether you’re a chromatographer, lab professional, or researcher you'll discover inspiring content!</p><p>You can find <strong>Concentrating on Chromatography Podcast </strong>in podcast apps:</p><ul><li><a target="_blank" rel="noopener noreferrer" href="https://open.spotify.com/show/1wZg67rCjoGju4yhqxI3tL"><strong>Spotify</strong></a></li><li><a target="_blank" rel="noopener noreferrer" href="https://podcasts.apple.com/tz/podcast/concentrating-on-chromatography/id1788429185"><strong>Apple podcast</strong></a></li><li><a target="_blank" rel="noopener noreferrer" href="https://podscan.fm/podcasts/concentrating-on-chromatography"><strong>podscan</strong></a></li></ul><p>and on <a target="_blank" rel="noopener noreferrer" href="https://www.youtube.com/@ChromatographyTalk/videos"><strong>YouTube channel</strong></a></p></blockquote>

 Fighting Antibiotic Resistance with LC-MS: Targeting Bacterial Cell Walls

<p><strong>This study compares a commercial ELISA assay with a validated UHPLC-MS/MS method for determining total ergot alkaloids in wheat and durum samples. In addition to method performance, the authors investigated cross-reactivity and matrix effects to explain differences between immunoassay and chromatographic results.</strong></p><p>While ELISA demonstrated good repeatability and is well suited for high-throughput screening, systematic biases were observed due to compound- and matrix-dependent cross-reactivity. The findings support ELISA as an effective screening tool, whereas UHPLC-MS/MS remains the preferred method for accurate quantification and regulatory confirmation of ergot alkaloids.</p><blockquote><h4><strong>The original article</strong></h4><h4><a target="_blank" rel="noopener noreferrer" href="https://pubs.acs.org/doi/10.1021/acs.jafc.6c05234"><strong>Comparative Evaluation of Total Ergot Alkaloids Determination by ELISA and UHPLC-MS/MS in Wheat: Implications of Cross-Reactivity and Matrix Effects</strong></a></h4><p>Chamali Kodikara*, Dainna Drul, Nandika Bandara, and Sheryl A. Tittlemier*</p><p>J. Agric. Food Chem. 2026, 74, 25, 20015–20027</p><p><a target="_blank" rel="noopener noreferrer" href="https://doi.org/10.1021/acs.jafc.6c05234">https://doi.org/10.1021/acs.jafc.6c05234</a></p><h6>licensed under <a target="_blank" rel="noopener noreferrer" href="https://creativecommons.org/licenses/by/4.0/"><strong>CC-BY 4.0</strong></a></h6></blockquote><p><strong>Selected sections from the article follow. Formats and hyperlinks were adapted from the original.</strong></p><div><p>Ergot alkaloids (EAs) are a structurally diverse group of indole-derived mycotoxins produced primarily by phytopathogenic fungi of the genus <i>Claviceps</i>, most notably <i>Claviceps purpurea</i>. These fungi infect the flowering heads of cereal crops, replacing the developing grain with hardened fungal structures (sclerotia) that contain ergot alkaloids. Cereals such as rye, wheat, barley, oats, and triticale are particularly susceptible, with infection severity strongly influenced by environmental conditions during flowering, including rainfall and humidity. <a href="javascript:void(0);">(1−3)</a> Although improvements in agronomic practices and grain cleaning have reduced the prevalence of visible ergot sclerotia, ergot alkaloids can persist in cereals and cereal-based foods even in the absence of detectable sclerotia. This may occur because sclerotia can fragment during harvesting, handling, cleaning, or milling, producing small particles that are not visually detected but may still contribute ergot alkaloids to grain or processed products, continuing to pose food and feed safety concerns. <a href="javascript:void(0);">(4)</a> <a href="javascript:void(0);">(5)</a> Ergot alkaloids share a common tetracyclic ergoline backbone and occur as structurally related compounds, including ergometrine, ergotamine, ergosine, ergocristine, ergocryptine, and ergocornine, together with their corresponding C-8 epimers. These epimers occur as <i>R-</i> and <i>S-</i>configurations, commonly referred to as -ine and -inine forms, respectively. Epimerization at the C-8 position can occur during fungal metabolism and during sample extraction, storage, and analysis, complicating accurate quantification. <a href="javascript:void(0);">(6,7)</a> Although S-epimers were historically considered biologically inactive, recent evidence indicates that they may contribute to overall exposure and toxicity, supporting their inclusion in analytical and regulatory frameworks. <a href="javascript:void(0);">(3)</a></p><p>Dietary exposure to elevated levels of ergot alkaloids has been associated with a range of adverse health effects, collectively referred to as ergotism, including vasoconstriction, ischemia, neurological disturbances, and hallucinations. <a href="javascript:void(0);">(3)</a> These effects arise primarily from interactions with adrenergic, serotonergic, and dopaminergic receptors. <a href="javascript:void(0);">(8−10)</a> In response, regulatory limits for ergot alkaloids in food have been introduced or revised in several jurisdictions. In the European Union, maximum levels have been established for the sum of 12 ergot alkaloids in cereal milling products under Regulation (EU) 2021/1399, which was subsequently consolidated under Regulation (EU) 2023/915. This sum includes ergometrine, ergosine, ergotamine, ergocornine, ergocryptine, and ergocristine together with their corresponding -inine epimers, increasing the demand for reliable analytical methods to support routine monitoring and enforcement. <a href="javascript:void(0);">(1,11)</a></p><p>Liquid chromatography-tandem mass spectrometry (LC-MS/MS), particularly ultrahigh performance liquid chromatography-tandem mass spectrometry (UHPLC-MS/MS), is widely regarded as the reference technique for confirmatory determination of ergot alkaloids due to its sensitivity, selectivity, and ability to resolve epimeric forms. Recent methods routinely achieve low microgram-per-kilogram limits of quantification across a range of cereal matrices. <a href="javascript:void(0);">(12−17)</a> However, LC-MS/MS approaches are resource-intensive and typically confined to centralized laboratories, limiting their suitability for high-throughput screening or rapid decision-making in grain-handling and quality-control settings. Consequently, rapid screening methods, most notably enzyme-linked immunosorbent assays (ELISAs), are commonly employed as complementary tools to chromatographic analysis. <a href="javascript:void(0);">(18−21)</a> ELISAs offer reduced analysis time, simplified workflows, and lower per-sample costs, making them attractive for preliminary screening and sample triage. <a href="javascript:void(0);">(22−24)</a></p><p>In contrast to other regulated mycotoxins, however, the commercial availability of ELISAs for ergot alkaloids is limited. While multiple kits have been evaluated, performance has varied substantially across assays and matrices. <a href="javascript:void(0);">(1)</a> At present, the Randox Food Diagnostics Ergot Alkaloids ELISA is the only widely marketed microplate ELISA that quantifies the sum of 12 major ergot alkaloids, comprising ergometrine, ergosine, ergotamine, ergocornine, ergocryptine, and ergocristine together with their corresponding -inine epimers, consistent with the analyte scope used for EU maximum levels for ergot alkaloids in specified cereal products. <a href="javascript:void(0);">(25)</a></p><p>In addition, cereal matrix effects may influence antibody binding and signal generation, potentially resulting in biased quantification relative to LC-MS/MS reference methods. These limitations are particularly relevant given the compositional variability of ergot alkaloids across cereal species, geographic regions, and growing conditions. <a href="javascript:void(0);">(12)</a> Recent reviews emphasize the need for systematic, matrix-specific evaluation of immunoassay performance before such methods can be confidently integrated into regulatory or industrial monitoring programs. <a href="javascript:void(0);">(1,6,11)</a> However, independent evaluations of commercially available ergot alkaloid ELISAs, particularly those benchmarked against validated UHPLC-MS/MS methods using naturally infested wheat samples, remain limited.</p><p>In this context, we hypothesized that measurements of total ergot alkaloids obtained using a commercially available ELISA are influenced by matrix effects and antibody cross-reactivity, resulting in systematic differences compared to UHPLC-MS/MS, which provides compound-specific quantification. To test this, the objective of the present study was to conduct a comprehensive performance evaluation of a commercially available ELISA for the detection of ergot alkaloids in wheat, using UHPLC-MS/MS as a reference method. Specific aims included assessment of repeatability and intermediate precision, extraction efficiency and extract stability, the influence of test portion mass, method accuracy relative to UHPLC-MS/MS, and characterization of antibody cross-reactivity toward individual ergot alkaloids in both solvent and wheat matrix. The results provide data-driven guidance on the appropriate use of ELISA-based screening within tiered analytical strategies for regulatory monitoring and food safety decision-making.</p><h4><strong>2. Materials and Methods</strong></h4><h5><strong>2.3. UHPLC-MS/MS Reference Method</strong></h5><p>UHPLC-MS/MS analysis of ergot alkaloids was performed using the validated Canadian Grain Commission (CGC) method described by Tittlemier et al. (2015). Briefly, ground grain (10 g) was extracted with 50 mL of acetonitrile/aqueous ammonium carbonate (84:16, v/v; 3.03 mM ammonium carbonate) to provide alkaline extraction conditions, then shaken for 30 min and centrifuged at 2450<i>g</i> for 10 min at room temperature. An aliquot of extract (464 μL) was combined with internal standard solution (dihydroergotamine; 20 μL of 4 ng/μL) and aqueous ammonium carbonate (1536 μL), vortex-mixed, and filtered through a 0.45 μm polytetrafluoroethylene syringe filter before injection into the UHPLC-MS/MS system. As in the validated reference method, dihydroergotamine was added after extraction to correct for postextraction analytical variability; extraction recovery was evaluated during method validation. Chromatographic separation was achieved using a reversed-phase <strong>BEH C18 column</strong> (2.1 × 100 mm, 1.7 μm; Waters, Milford, MA, USA) with the autosampler maintained at 15 °C and the column at 30 °C. Mobile phases consisted of (A) 3.03 mM aqueous ammonium carbonate and (B) acetonitrile. The gradient program was as follows: initial, 75% A/25% B; 1.00 min, 60% A/40% B; 5.00 min, 40% A/60% B; 8.00 min, 22% A/78% B; 10.50–11.00 min, 10% A/90% B; and 13.00–16.00 min, 75% A/25% B, with a total run time of 16 min. Detection was performed using positive electrospray ionization in multiple reaction monitoring mode on a <a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/products/554"><strong>Waters Xevo triple quadrupole mass spectrometer</strong></a> (Waters, Milford, MA, USA). Under the validated chromatographic conditions, dihydroergotamine did not coelute with ergotamine, ergotaminine, or the other target ergot alkaloids. Identification and quantification were based on retention-time criteria and compound-specific MRM transitions, thereby minimizing potential isotopic or coelution interference. Precautions to minimize epimerization, including controlled handling of standards and extracts and reduced light exposure, were followed as described in the reference method. UHPLC-MS/MS quantification was performed using analyte-specific, matrix-matched calibration curves prepared according to the validated CGC reference method, with calibration levels of 0.046, 0.2, 1, 2, 10, and 20 μg/kg. Dihydroergotamine was used as the internal standard, and concentrations were calculated from internal-standard-normalized analyte responses with method dilution factors applied. Total ergot alkaloid concentration was calculated as the arithmetic sum of all quantified ergot alkaloids and epimers included in the validated method. <a href="javascript:void(0);">(26)</a></p><h4><strong>3. Results and Discussion</strong></h4><h5><strong>3.4. Comparison of ELISA and UHPLC-MS/MS Results</strong></h5><p>For method comparison, UHPLC-MS/MS was used as the chemical reference method, providing compound-specific quantification of individual ergot alkaloids and their epimers. A total ergot alkaloid concentration was calculated as the arithmetic sum of all quantified alkaloids included in the method. This summed value represents a chemically defined total based on compound-specific quantification of each analyte, rather than an immunoreactivity-weighted response governed by antibody affinity. As such, the UHPLC-MS/MS total serves as a chemically defined comparator against which the immunoreactivity-weighted ELISA response can be interpreted. Agreement between the commercial ELISA and the validated UHPLC-MS/MS reference method was evaluated using wheat and durum wheat cargo samples (<i>n</i> = 102) collected over multiple crop years (<a target="_blank" rel="noopener noreferrer" href="https://pubs.acs.org/doi/10.1021/acs.jafc.6c05234#fig5">Figure 5</a>). Linear regression analysis demonstrated a positive association between ELISA and UHPLC-MS/MS results across the full concentration range, indicating that the ELISA was responsive to increasing ergot alkaloid levels. However, the regression slope deviated substantially from unity, with ELISA results systematically lower than UHPLC-MS/MS values, indicating an approximate 2-fold underestimation.&nbsp;</p><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/J_Agric_Food_Chem_2026_74_25_20015_20027_Figure_5_Linear_regression_analysis_comparing_total_ergot_alkaloid_concentrations_determined_by_ELISA_and_UHPLC_MS_MS_5d7b5c5c61.jpeg" alt="J. Agric. Food Chem. 2026, 74, 25, 20015–20027: Figure 5. Linear regression analysis comparing total ergot alkaloid concentrations determined by ELISA and UHPLC-MS/MS for wheat and durum wheat cargo samples (n = 102). Each point represents the mean of duplicate measurements (extracted from the same test portion) ELISA determinations for an individual cargo sample, plotted against the corresponding UHPLC-MS/MS result. Statistical analysis was performed using linear regression." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_J_Agric_Food_Chem_2026_74_25_20015_20027_Figure_5_Linear_regression_analysis_comparing_total_ergot_alkaloid_concentrations_determined_by_ELISA_and_UHPLC_MS_MS_5d7b5c5c61.jpeg 197w," sizes="100vw" width="992" height="784"></p><p>Bland-Altman analysis confirmed this proportional bias, showing a consistent negative mean difference between ELISA and UHPLC-MS/MS results across the concentration range (<a target="_blank" rel="noopener noreferrer" href="https://pubs.acs.org/doi/10.1021/acs.jafc.6c05234#fig6">Figure 6</a>). Importantly, the magnitude of disagreement did not increase randomly with concentration; rather, it reflected a systematic offset, indicating that the discrepancy was not driven by analytical imprecision or outliers but by fundamental differences in method response characteristics. This pattern indicates method-level bias rather than random analytical variability, which may arise from combined workflow effects including extraction, matrix response, and immunochemical cross-reactivity. The magnitude of the ELISA-UHPLC-MS/MS bias was substantially greater than the intermediate precision observed for the ELISA quality-control material, supporting the interpretation that the disagreement reflects systematic differences in method response rather than insufficient ELISA repeatability. Such behavior is well documented when immunoassays are compared with chromatographic methods for complex mycotoxin mixtures, including ergot alkaloids and other structurally diverse mycotoxins in cereals and feed. <a href="javascript:void(0);">(28,30,33)</a> These studies consistently report matrix- and profile-dependent bias in ELISA relative to LC-MS/MS, arising from differential antibody cross-reactivity and the summed immunoreactivity response of the assay, because ELISA produces an immunoreactivity-weighted total that depends on antibody affinity toward individual alkaloids and their relative abundance in the sample, whereas UHPLC-MS/MS quantifies compound-specific concentrations of each alkaloid and its epimers using compound-specific transitions and response factors, and total ergot alkaloid concentration is calculated as the sum of these targeted analytes. In contrast, ELISA produces an immunoreactivity-weighted total, determined by antibody affinity toward individual alkaloids and their competitive binding behavior within the assay format. Consequently, agreement between ELISA and UHPLC-MS/MS depends not only on total concentration, but also on the relative distribution of individual alkaloids within each sample. Although differences in extraction solvent composition and pH may influence ergot alkaloid recovery, stability, and epimeric distribution, each method was applied according to its intended analytical workflow. The ELISA was performed using the manufacturer-recommended acidic methanol/water extraction, whereas UHPLC-MS/MS was performed using the validated acetonitrile/aqueous ammonium carbonate extraction procedure. Therefore, the comparison reflects the performance of the complete methods as used in practice, rather than an isolated comparison of extraction solvents. The sequential extraction experiment further showed that repeating the ELISA extraction under the same kit conditions did not substantially increase the ELISA-measured response, supporting the adequacy of the kit extraction within the ELISA workflow while recognizing that it does not directly compare acidic and ammonium carbonate-based extraction conditions. Previous ELISA-LC-MS/MS comparison studies for ergot alkaloids have reported both under- and overestimation depending on assay design and matrix, including differences in alkaloid profile, cereal type, and antibody cross-reactivity. Turner et al. observed substantial overestimation of ergot alkaloids by ELISA relative to HPLC in rye field samples, attributed to strong antibody cross-reactivity toward alkaloids prevalent in rye matrices. Similar profile-dependent bias has also been reported in interlaboratory and method-comparison studies, <a href="javascript:void(0);">(33)</a> demonstrating that this behavior is not unique to a single data set.</p><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/J_Agric_Food_Chem_2026_74_25_20015_20027_Figure_6_Bland_Altman_plot_comparing_ELISA_and_UHPLC_MS_MS_measurements_of_total_ergot_alkaloids_1be40cce50.jpeg" alt="J. Agric. Food Chem. 2026, 74, 25, 20015–20027: Figure 6. Bland-Altman plot comparing ELISA and UHPLC-MS/MS measurements of total ergot alkaloids for wheat and durum wheat cargo samples (n = 102)." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_J_Agric_Food_Chem_2026_74_25_20015_20027_Figure_6_Bland_Altman_plot_comparing_ELISA_and_UHPLC_MS_MS_measurements_of_total_ergot_alkaloids_1be40cce50.jpeg 230w," sizes="100vw" width="1437" height="977"></p><p>This study provides a comprehensive evaluation of a commercially available ELISA for total ergot alkaloids, using wheat samples as the primary application matrix, and integrates performance metrics with mechanistic insights into method behavior. While the assay demonstrated strong repeatability, calibration stability, and robustness to procedural factors such as test portion mass and extraction conditions, systematic differences relative to UHPLC-MS/MS were consistently observed. By linking these differences to compound-specific antibody cross-reactivity and matrix-dependent signal modulation, this work clarifies that ELISA-derived results reflect an immunochemically weighted response rather than a stoichiometric total. This distinction is critical for the appropriate interpretation of ELISA data and supports its use within tiered analytical strategies as a rapid screening tool, complemented by UHPLC-MS/MS for confirmatory quantification and regulatory decision-making.</p></div>

Comparative Evaluation of Total Ergot Alkaloids Determination by ELISA and UHPLC-MS/MS in Wheat: Implications of Cross-Reactivity and Matrix Effects

<p><strong>In the week of July 6, the following webinars await you in the field of liquid phase and especially HPLC and LC/MS.</strong></p><blockquote><h4><a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/webinars"><strong>👉 DETAILS AND REGISTRATION IN THE WEBINARS SECTION</strong></a></h4><p><strong>👉 Search and filter in the largest global database</strong> with over <strong>5 500 GC, LC, MS and spectroscopy webinars</strong>.</p></blockquote><h5><strong>1. Waters Corporation: Characterizing Next-Generation Antibody Therapeutics: LC Strategies for Multispecifics, ADCs &amp; AOCs</strong></h5><ul><li>Tu, 7.7.2026 11:00 CEST</li><li><a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/webinar/6317">Registration</a></li></ul><p>In this webinar, we share a pragmatic characterization roadmap using LC-based methods across intact, subunit, and peptide-mapping workflows.</p><h5><strong>2. Separation Science: Advances in Antibiotic Extraction from Water: An Automated SPE Approach</strong></h5><ul><li>We, 8.7.2026 09:00 CEST</li><li><a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/webinar/6319">Registration</a></li></ul><p>Streamline water analysis workflows and gain practical insights for modern environmental monitoring.</p><h5><strong>3. SCIEX: Analysis of per- and polyfluoroalkyl substances (PFAS) in food substances using LC-MS/MS solutions</strong></h5><ul><li>We, 8.7.2026 15:00 CEST</li><li><a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/webinar/6322">Registration</a></li></ul><p>Master PFAS analysis in food safety. Learn how SCIEX LC-MS/MS solutions and QTRAP workflows overcome analytical challenges to ensure accurate and compliant result confirmation.</p><h5><strong>4. Wiley: From liquids to solids: expand what your UV-Vis-NIR Spectrometer can do</strong></h5><ul><li>We, 8.7.2026 16:00 CEST</li><li><a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/webinar/6367">Registration</a></li></ul><p>Discover how the Agilent Cary UV-Vis-NIR spectrometer, together with Specac accessories, enables reliable analysis of liquids, powders, films, pellets, and highly scattering solid samples.</p><h5><strong>5. Thermo Fisher Scientific: Advanced tools to maximize your sample throughput with Chromeleon Software and Vanquish UHPLC</strong></h5><ul><li>Th, 9.7.2026 11:00 CEST</li><li><a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/webinar/6326">Registration</a></li></ul><p>Learn how Chromeleon software and the Vanquish Duo UHPLC system streamline sample analysis, automate routine workflows, increase throughput, and reduce errors through practical demonstrations.</p><h5><strong>6. Bruker: 4D-Metabolomics™ in Action: Real-World Strategies for Deep Discovery and Confident Annotation</strong></h5><ul><li>Th, 9.7.2026 17:00 CEST</li><li><a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/webinar/6328">Registration</a></li></ul><p>Explore how next-generation mass spectrometry technologies, ion mobility, and computational tools are helping researchers move from complex datasets to confident insight.</p><h5><strong>7. Waters Corporation: Cracking the Code on Nitrogen Methods</strong></h5><ul><li>Th, 9.7.2026 17:00 CEST</li><li><a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/webinar/6327">Registration</a></li></ul><p>Learn the fundamentals of nitrogen analysis for wastewater testing. Explore analytical methods, QA/QC, sample handling, compliance reporting, and troubleshooting for accurate environmental monitoring.</p><h5><strong>8. SelectScience: Next-generation metabolomics: From annotation to discovery at scale</strong></h5><ul><li>Th, 9.7.2026 17:00 CEST</li><li><a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/webinar/6281">Registration</a></li></ul><p>Explore how next-generation mass spectrometry, ion mobility, and advanced computational tools accelerate confident small-molecule annotation and discovery in metabolomics.</p><h5><strong>9. SCIEX: LC-MS/HRMS Approaches for Monitoring Organic Micropollutants in Wastewater and Environmental Systems</strong></h5><ul><li>Fr, 10.7.2026 15:00 CEST</li><li><a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/webinar/6370">Registration</a></li></ul><p>Discover how advanced LC-MS/HRMS workflows enable trace-level detection of organic micropollutants, suspect and non-target screening, and environmental monitoring of wastewater systems.</p>

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Thermo Scientific™ GENESYS™ 30 Vis Spectrophotometer

Perform both basic and complex analyses with the Thermo Scientific™ GENESYS™ 30 Visible Spectrophotometer, an intuitive, reliable solution for education or in the lab.

<h3><strong>Designed for Users, Designed for Results</strong></h3><p><strong>The Thermo Scientific ™ GENESYS ™ 30 is the most thoughtfully designed spectrophotometer available today. Its high resolution color display, large sample compartment, and data export options meet modern expectations for students, educators and lab technicians. The GENESYS 30 blends the simplicity to satisfy novice students with the capabilities that labs need for accurate quantitative analysis.</strong></p><h4><strong>System Benefits:</strong></h4><ul><li>Large sample compartment with front access simplifies sample handling</li><li>High resolution color display</li><li>Outstanding durability in challenging environments thanks to:</li><li>Waterproof screen</li><li>Sealed, rubberized keypad</li><li>Removable, washable sample compartment</li><li>Sloping surfaces that shed spills</li><li>Chemical resistant PC-ABS covers</li><li>3A linear measurement range for concentrated solutions</li><li>Multi-point quant offers higher measurement confidence</li><li>Traditional monochromator paired with advanced electronics delivers outstanding stability</li><li>Scan mode for spectral analysis and interpretation</li><li>Data any way you want it - excellent snap-on thermal printer or USB data export</li><li>Expanded capabilities with optional VISIONlite software</li><li>2-year warranty</li></ul><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Thermo_Scientific_GENESYS_30_Vis_Spectrophotometer_4_4442fcccc5.jpg" alt="Thermo Scientific™ GENESYS™ 30 Vis Spectrophotometer." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Thermo_Scientific_GENESYS_30_Vis_Spectrophotometer_4_4442fcccc5.jpg 195w," sizes="100vw" width="2103" height="1685"></p><h3><strong>Modern Power meets Traditional Simplicity</strong></h3><h4><strong>Live Display and Concentration Mode</strong></h4><p>Replicate the familiar display of absorbance or transmittance, and single standard concentration measurements of prior generation instruments in the GENESYS 30 Live Display mode. All your parameters are set and kept in view on a single screen as the calculated concentration updates every two seconds.</p><h4><strong>Fixed Mode</strong></h4><p>Apply a factor to an absorbance measurement at one wavelength, or measure at two wavelengths with two factors and perform addition, subtraction or division of the values. Display results for up ten samples then print, save or clear your data before going on to the next sample series.</p><h4><strong>Quantitative Analysis with up to Six Standards</strong></h4><p>Go beyond the basic spectrophotometer by recording and storing up to six standards to calibrate your method. Choose a linear fit with an intercept or forced through zero. Name and save your method on the instrument or back it up to a USB memory device. All data are recorded with an index number (S1 – S10) and a time stamp. The result table accommodates up to ten samples for you to print or save before going on to the next sample series or analysis.</p><h4><strong>Go negative!</strong></h4><p>Some methods call for blanking the instrument on a dark standard and reading absorbance in the negative range. Most single beam instruments cannot measure below -0.3A. GENESYS 30 uses advanced signal processing to deliver stable, reliable results down to -3A.</p><h4><strong>Simple to Sophisticated:</strong></h4><p>The GENESYS 30 has the flexibility to perform routine analysis one minute and complex analytical methods the next. The custom Analyzer mode enables you to build your formula with up to six wavelengths, six factors, standard arithmetic operators and absorbance values raised to the 2nd or 3rd power. Use the included Windows® application to design your method. Transfer the method to your GENESYS 30 and run it with the touch of a button. Analyzer mode shows your method name and a continuous display of your results. Technicians can write the result down or print it with a time stamp using the printer accessory.</p><h4><strong>Smarter by Design:&nbsp;</strong></h4><p>GENESYS 30 offers the convenient SmartStart™ feature that has delighted GENESYS 10S users since its introduction. The SmartStart menu gives you direct access to your<strong> 12 favorite methods</strong>. Allowing you to work smarter, not harder.</p><h4><strong>Snap on Printer:&nbsp;</strong></h4><p>Fast and clear printing when you need it. Add printing capability to your instrument. The snap-on thermal printer installs<br>in moments.&nbsp;</p><h4><strong>Quick and Simple Lamp Replacement:&nbsp;</strong></h4><p>No need to remove the cover, just loosen two screws, slide out the old lamp fitting and slide a new one back in. Everything is pre-aligned, just let the new lamp warm up and go back to work.</p><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Thermo_Scientific_GENESYS_30_Vis_Spectrophotometer_3_82082740d3.jpg" alt="Thermo Scientific™ GENESYS™ 30 Vis Spectrophotometer: Quick and simple lamp replacement." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Thermo_Scientific_GENESYS_30_Vis_Spectrophotometer_3_82082740d3.jpg 197w," sizes="100vw" width="1737" height="1376"></p><h3><strong>At Home in the Instructional Laboratory</strong></h3><p><strong>GENESYS spectrophotometers are core teaching instruments in chemistry and biology laboratories around the world. The Thermo Scientific™ GENESYS™ 30 allows students and teachers to focus on examining scientific data and learning scientific concepts instead of learning instrumentation.</strong></p><h4><strong>Easy to Teach, Easy to Learn&nbsp;</strong></h4><p>Clearly labeled, uniquely colored keys simplify instrument operation. New users require little or no training.</p><h4><strong>Get Working – Faster!</strong></h4><p>Large, color digital screen and numeric keypad help you set up methods faster. Simplified software interface gives users maximum control and efficiency.</p><h4><strong>Designed for Today, Built for Tomorrow – Inside and Out!</strong></h4><p>On the inside, GENESYS 30 uses advanced optics, electronics and signal processing to deliver superior measurement performance for its class. On the outside, GENESYS 30 presents a tough exterior built to last for years in the most demanding environments.</p><ul><li>Resistant to spills</li><li>Removable and washable sample compartment</li><li>The sample compartment lid<ul><li>stays where you put it - open or closed</li><li>has a magnetic latch to hold it closed during measurements</li><li>is customer-replaceable should it get broken through abuse</li></ul></li></ul><h4><strong>Simple and Familiar</strong></h4><p>Open the Live Display application with a single button-press to monitor the absorbance or transmittance live – just like a GENESYS 20 or SPECTRONIC 20. The spectrum indicator shows the color of light being absorbed.</p><h4><strong>Special support for OD 600 and test-tube measurements</strong></h4><p>For measurements in test-tubes, the test-tube holder accessory accommodates 100mm x 13mm test-tubes with the lid closed. Or add the tall test-tube adaptor to use larger diameter, taller tubes (up to 150mm, 6 inches) with the lid open. Special OD600 mode for cell growth measurements. Select it from the Home menu and go! No method setup required.</p><h4><strong>Scanning: Simple and Fast</strong></h4><p>Find peaks in the spectrum of your sample by scanning across the spectrum in any range from 325nm to 1100nm with 1nm precision and noise-free peaks up to 3A. Use the cursor and zoom controls to identify λ<sub>max</sub>.</p><h4><strong>Data you can use</strong></h4><ul><li>Print your scan and stick it into a lab notebook using the printer accessory and optional self-stick printer paper.</li><li>Save the data points of your scan to a USB memory device and load the data into a spreadsheet or graphing program for optimal reporting.</li></ul><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Pragolab_Thermo_Scientific_Genesys_30_USB_stick_A_c45fd86787.jpg" alt="Thermo Scientific™ GENESYS™ 30 Vis Spectrophotometer: USB stick." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Pragolab_Thermo_Scientific_Genesys_30_USB_stick_A_c45fd86787.jpg 181w," sizes="100vw" width="543" height="469"></p><h3><strong>VISIONlite Software</strong></h3><p><strong>For users who need the power of computerized data analysis, optional Thermo Scientific™ VISIONlite™ software pairs a simple user</strong><br><strong>interface with a wider range of options for experiment design and data analysis.</strong></p><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Thermo_Scientific_Genesys_spectrophotometers_ae282fe726.jpg" alt="Thermo Scientific - Genesys spectrophotometers: VISIONlite Software." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Thermo_Scientific_Genesys_spectrophotometers_ae282fe726.jpg 239w," sizes="100vw" width="1422" height="928"></p><h4><strong>Scan Application:</strong></h4><ul><li>Automated peak pick and label</li><li>Overlay spectra and print the results</li></ul><h4><strong>Kinetics Application:</strong></h4><ul><li>Data intervals as low as 1 second</li><li>Enzyme rate calculation with factor</li></ul><h4><strong>Quantitative Analysis:</strong></h4><ul><li>Up to 100 standards</li><li>Linear, quadratic or cubic data fits</li></ul><h4><strong>Performance Verification:</strong></h4><ul><li>Full suite of tests to verify performance of your instrument</li><li>Pre-loaded pass/fail limits specific to your instrument</li></ul><h3><strong>GENESYS 30 Accessories</strong></h3><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Thermo_Scientific_GENESYS_30_Vis_Spectrophotometer_59d912ddd3.jpg" alt="Thermo Scientific™ GENESYS™ 30 Vis Spectrophotometer: GENESYS 30 Accessories." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Thermo_Scientific_GENESYS_30_Vis_Spectrophotometer_59d912ddd3.jpg 243w," sizes="100vw" width="1444" height="929"></p><h3><strong>GENESYS 30 Vis Spectrophotometer Specifications</strong></h3><ul><li><strong>Baseline Flatness:</strong> &lt;0.003 A, 325 to 1000 nm smoothed</li><li><strong>Compartments:</strong>&nbsp;<ul><li>Accessible from top, front or side</li><li>Able to accommodate cells up to 100mm pathlength</li><li>Available accessory for test-tubes up to 25mm diameter and 150mm height</li><li>Removable, washable sample compartment liner with magnetic placement and hold-down</li></ul></li><li><strong>Connections:</strong> USB-A, USB-B</li><li><strong>Description:</strong> GENESYS 30 VIS Spectrophotometer with EU Power Cable</li><li><strong>Dimensions (L x W x H):</strong> 35.5 x 38.5 x 19.5 cm</li><li><strong>Drift:</strong> &lt;0.002 A/hr. (At 500 nm after 2 hr. warmup.) Typical &lt;0.004 A/hr. after 1 hr. warmup</li><li><strong>Electrical Requirements:</strong> External AC to DC converter. Voltage and Frequency (Hz) selected automatically, 100/250 V, 50/60 Hz</li><li><strong>Keypad:</strong> Tactile rubber 23 keys with numeric keypad</li><li><strong>Lamp Life:</strong> 1000 hrs.</li><li><strong>Noise:</strong> (RMS at 500nm. 60 consecutive measurements)<ul><li>≤0.001A at 0A</li><li>≤0.001A at 1A</li><li>≤0.002A at 2A</li></ul></li><li><strong>Optical Design:</strong> Single Beam</li><li><strong>Photometric Accuracy:</strong> ±0.002 A (0 to 0.3 A), 0.5% of ABS reading (0.301 A to 2.5 A)</li><li><strong>Photometric Display:</strong> -3A to +3A, 0 to 200 000 %T, 0 to 9 999 999 C</li><li><strong>Photometric Range:</strong> -3A to +3A</li><li><strong>Photometric Repeatability:</strong> ± 0.002 A (Measured at 1.0 A at 546 nm)</li><li><strong>Printer Included:</strong> Thermal printer accessory with 80 mm width paper</li><li><strong>Scan Speed:</strong> Automatic – up to 1200 nm/min.</li><li><strong>Stray Light:</strong> &lt;0.1%T at 340 nm and 400 nm</li><li>Measured using glass cutoff filters or NaNO₂ at 340 nm</li><li><strong>Warranty:</strong> 2 years standard with 1 year extension upon registration of the instrument with Thermo Fisher Scientific within 6 months of purchase</li><li><strong>Wavelength Accuracy:</strong> ±2 nm</li><li><strong>Wavelength Data Interval:</strong> 1 nm</li><li><strong>Wavelength Repeatability:</strong> &lt; ±1 nm</li><li><strong>Weight (Metric):</strong> 7.5 kg</li><li><strong>Detector Type:</strong> Silicon Photodiode</li><li><strong>Display:</strong> 5 in. 32-bit Color Display, 800 x 480 pixels</li><li><strong>Languages:</strong> English, French, German, Spanish, Italian, Portuguese, Russian, Chinese, Japanese, Korean, Thai, Arabic</li><li><strong>Wavelength Range:</strong> 325 to 1100 nm</li><li><strong>Unit Size:</strong> Each</li></ul>

Thermo Scientific™ GENESYS™ 40 Vis Spectrophotometer

Perform routine measurements for quality control or teaching in the chemistry classroom with rugged Thermo Scientific™ GENESYS 40 Vis Spectrophotometer.

<h4><strong>GENESYS - developed for users, designed for results</strong></h4><p>Built on the foundation of 60 years of spectroscopy experience, the next generation Thermo Scientific™ GENESYS™ family of Vis/UV-Vis spectrophotometers provides exceptional performance with a contemporary design.</p><ul><li>Start taking measurements immediately with the simple-to-use, high-resolution, color-display start menu</li><li>Exchange samples with ease with large, accessible sample compartment</li><li>Save your data fast any way you want it with multiple data-export options</li></ul><p>There is a GENESYS spectrophotometer you can count on to provide accurate, reproducible results for your application requirements.</p><h3><strong>GENESYS 40 Vis Spectrophotometer</strong></h3><p><strong>For teaching labs, research labs and industrial QC requiring robust construction, ease-of-use and software capability.</strong></p><h5><strong>Advanced usability, modern functionality</strong></h5><ul><li>7-inch, high-resolution touchscreen interface</li><li>Local control increases speed and reliability for routine analysis</li><li>Large sample compartment with front access simplifies sample handling</li><li>Wi-Fi ready - save data and methods, print results from on-board control</li></ul><h5><strong>Designed for</strong></h5><ul><li>Chemistry labs</li><li>Routine measurements</li><li>Moderate throughput requirements</li></ul><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Pragolab_Thermo_Scientific_Genesys_40_Kyveta_A_7955a6a01a.jpg" alt="Thermo Scientific™ GENESYS™ 40 Vis Spectrophotometer" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Pragolab_Thermo_Scientific_Genesys_40_Kyveta_A_7955a6a01a.jpg 234w," sizes="100vw" width="1536" height="1024"></p><h3><strong>Walk-up convenience</strong></h3><p><strong>Simple-to-use, touchscreen user interface features the methods and applications most important to you.</strong></p><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Thermo_Scientific_Genesys_spectrophotometers_ae282fe726.jpg" alt="Thermo Scientific GENESYS Spectrophotometers" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Thermo_Scientific_Genesys_spectrophotometers_ae282fe726.jpg 239w," sizes="100vw" width="1422" height="928"></p><h5><strong>Flexible computer control</strong></h5><p>Optional VISIONlite software offers complete control from a Microsoft ® Windows ® computer providing applications for:</p><ul><li>Scanning</li><li>Quantitative analysis</li><li>Kinetics</li><li>Fixed wavelength measurements</li></ul><h3><strong>GENESYS 40 Vis Spectrophotometer Specifications</strong></h3><ul><li><strong>Baseline Flatness:</strong> ±0.005 A</li><li><strong>Compartments:</strong>&nbsp;<ul><li>Accessible from top, front or side</li><li>Able to accommodate cells up to 100mm cells</li><li>Available accessory for test tubes up to 25 mm diameter and 150 mm height</li><li>Removable, washable sample compartment liner with magnetic placement and hold-down</li></ul></li><li><strong>Connections:</strong>&nbsp;<ul><li>Single USB-A</li><li>Duplex USB-A</li><li>Export data to network or PC via USB, Ethernet, or Wi-Fi USB adaptor, available&nbsp;</li><li>Print via USB, Ethernet or Wi-Fi USB adaptor available</li></ul></li><li><strong>Dimensions (L x W x H):</strong> 35.5 x 38.5 x 19.5 cm</li><li><strong>Drift:</strong> (At 500 nm after 1 hour warmup) &lt;0.0010 A/hr.</li><li><strong>Electrical Requirements:</strong> External AC to DC converter. Voltage and Frequency (Hz) selected automatically, 100/240 V, 50/60 Hz</li><li><strong>Includes:</strong> GENESYS 40 Spectrophotometer</li><li><strong>Keypad:</strong> Touchscreen</li><li><strong>Lamp Life:</strong> &gt;1000 hrs.</li><li><strong>Noise:</strong> (RMS at 500 nm 60 consecutive measurements)<ul><li>≤0.00020A at 0A at 260 and 500 nm</li><li>≤0.00030A at 1A at 260 and 500 nm</li><li>≤0.00040A at 2A at 260 and 500 nm</li></ul></li><li><strong>Optical Design:</strong> Dual Beam</li><li><strong>Photometric Accuracy:</strong> ±0.002 A at 0.5 A, ±0.004 A at 1.0 A, ±0.008 A at 2.0 A</li><li><strong>Photometric Display:</strong> -3A to +5A</li><li><strong>Photometric Range:</strong> –3A to +3.5A</li><li><strong>Photometric Repeatability:</strong> ±0.001A at 1A measured at 1.0A at 546 nm</li><li><strong>Printer Included:</strong> Snap-on printer available</li><li><strong>Scan Speed:</strong> Automatic – up to 1800 nm/min.</li><li><strong>Stray Light:</strong> &lt;0.05%T at 340 nm and 400 nm</li><li><strong>Warranty:</strong> 2 yrs std + 1 yr ext upon reg</li><li><strong>Wavelength Accuracy:</strong> ±0.5 nm</li><li><strong>Wavelength Data Interval:</strong> 0.2 nm, 0.5 nm, 1 nm, 2 nm, 5 nm</li><li><strong>Wavelength Repeatability:</strong> &lt; ±0.2 nm</li><li><strong>Weight (Metric):</strong> 7.5 kg</li><li><strong>Detector Type:</strong> Dual Silicon Photodiodes</li><li><strong>Display:</strong> 7 in. High Definition Color Touchscreen, Fixed, 800 x 1280 pixels</li><li><strong>Light Source:</strong> Tungsten Halogen</li><li><strong>Spectral Bandwidth:</strong> 5 nm</li><li><strong>Wavelength Range:</strong> 325 to 1100 nm</li><li><strong>Unit Size:</strong> Each</li></ul>

Thermo Scientific™ GENESYS™ 140 Vis Spectrophotometer

Perform quantitative measurements in routine industrial QA/QC or university chemistry labs with the automated and network-capable Thermo Scientific™ GENESYS 140 Vis Spectrophotometer.

<h4><strong>GENESYS - developed for users, designed for results</strong></h4><p>Built on the foundation of 60 years of spectroscopy experience, the next generation Thermo Scientific™ GENESYS™ family of Vis/UV-Vis spectrophotometers provides exceptional performance with a contemporary design.</p><ul><li>Start taking measurements immediately with the simple-to-use, high-resolution, color-display start menu</li><li>Exchange samples with ease with large, accessible sample compartment</li><li>Save your data fast any way you want it with multiple data-export options</li></ul><p>There is a GENESYS spectrophotometer you can count on to provide accurate, reproducible results for your application requirements.</p><h3><strong>GENESYS 140 Vis Spectrophotometer</strong></h3><p><strong>For advanced teaching labs, R&amp;D, research and life science applications requiring higher throughput or temperature control.</strong></p><h5><strong>Elite performance, enhanced capabilities</strong></h5><ul><li>7-inch, high-resolution touchscreen interface</li><li>Local control increases speed and reliability for routine analysis</li><li>Large sample compartment with front access simplifies sample handling</li><li>Wi-Fi ready - save data and methods, print results from on-board control</li></ul><h5><strong>Designed for</strong></h5><ul><li>Chemistry labs</li><li>Routine measurements</li><li>Moderate throughput requirements</li></ul><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Pragolab_Thermo_Scientific_vis_spektrofotometr_genesys_140_titul_B_bd2a19cbcb.jpg" alt="Thermo Scientific GENESYS Spectrophotometers" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Pragolab_Thermo_Scientific_vis_spektrofotometr_genesys_140_titul_B_bd2a19cbcb.jpg 225w," sizes="100vw" width="516" height="358"></p><h3><strong>Walk-up convenience</strong></h3><p><strong>Simple-to-use, touchscreen user interface features the methods and applications most important to you.</strong></p><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Thermo_Scientific_Genesys_spectrophotometers_ae282fe726.jpg" alt="Thermo Scientific: Genesys spectrophotometers" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Thermo_Scientific_Genesys_spectrophotometers_ae282fe726.jpg 239w," sizes="100vw" width="1422" height="928"></p><h5><strong>Flexible computer control</strong></h5><p>Optional VISIONlite software offers complete control from a Microsoft ® Windows ® computer providing applications for:</p><ul><li>Scanning</li><li>Quantitative analysis</li><li>Kinetics</li><li>Fixed wavelength measurements</li></ul><h3><strong>GENESYS 140 Vis Spectrophotometer Specifications</strong></h3><ul><li><strong>Baseline Flatness:</strong> ±0.005 A</li><li><strong>Compartments:</strong> Accommodates (optional):<ul><li>8-position cell changer</li><li>4-position cell changer (long path cells)</li><li>Peltier thermostatted cell holder (20–60 °C)</li><li>Sipper accessory</li><li>Fiber optic probe coupler</li></ul></li><li><strong>Connections:</strong>&nbsp;<ul><li>Single USB-A</li><li>Duplex USB-A</li><li>Export data to network or PC via USB, Ethernet, or Wi-Fi USB adaptor, available&nbsp;</li><li>Print via USB, Ethernet or Wi-Fi USB adaptor available</li></ul></li><li><strong>Dimensions (L x W x H):</strong> 35.5 x 38.5 x 19.5 cm</li><li><strong>Drift:</strong> (At 500 nm after 1 hour warmup) &lt;0.0010 A/hr.</li><li><strong>Electrical Requirements:</strong> External AC to DC converter. Voltage and Frequency (Hz) selected automatically, 100/240 V, 50/60 Hz</li><li><strong>Includes:</strong> Genesys 140 Spectrophotometer</li><li><strong>Keypad:</strong> Touchscreen</li><li><strong>Lamp Life:</strong> &gt;1000 hrs.</li><li><strong>Noise:</strong> (RMS at 500 nm 60 consecutive measurements)<ul><li>≤0.00020A at 0A at 260 and 500 nm</li><li>≤0.00030A at 1A at 260 and 500 nm</li><li>≤0.00040A at 2A at 260 and 500 nm</li></ul></li><li><strong>Optical Design:</strong> Dual Beam</li><li><strong>Photometric Accuracy:</strong> ±0.002A at 0.5A, ±0.004A at 1.0 A±0.008 A at 2.0A</li><li><strong>Photometric Display:</strong> -3A to +5A</li><li><strong>Photometric Range:</strong> –3A to +3.5A</li><li><strong>Photometric Repeatability:</strong> ±0.001 A at 1 A measured at 1.0 A at 546 nm</li><li><strong>Printer Included:</strong> Snap-on printer available</li><li><strong>Scan Speed:</strong> Automatic – up to 1800 nm/min.</li><li><strong>Stray Light:</strong> &lt;0.05%T at 340 nm and 400 nm</li><li><strong>Warranty:</strong> 2 yrs std + 1 yr ext upon reg</li><li><strong>Wavelength Accuracy:</strong> ±0.5 nm</li><li><strong>Wavelength Data Interval:</strong> 0.2 nm, 0.5 nm, 1 nm, 2 nm, 5 nm</li><li><strong>Wavelength Repeatability:</strong> &lt; ±0.2 nm</li><li><strong>Weight (Metric):</strong> 7.5 kg</li><li><strong>Detector Type:</strong> Dual Silicon Photodiodes</li><li><strong>Display:</strong> 7 in. High Definition Color Touchscreen, Tiltable, 800 x 1280 pixels</li><li><strong>Light Source:</strong> Tungsten Halogen</li><li><strong>Spectral Bandwidth:</strong> 5 nm</li><li><strong>Wavelength Range:</strong> 325 to 1100 nm</li><li><strong>Unit Size:</strong> Each</li></ul>

Thermo Scientific™ GENESYS™ 50 UV-Vis Spectrophotometer

Perform routine measurements for quality control or teaching in the chemistry classroom with rugged Thermo Scientific™ GENESYS 50 UV-Vis Spectrophotometer.

<h4><strong>GENESYS - developed for users, designed for results</strong></h4><p>Built on the foundation of 60 years of spectroscopy experience, the next generation Thermo Scientific™ GENESYS™ family of Vis/UV-Vis spectrophotometers provides exceptional performance with a contemporary design.</p><ul><li>Start taking measurements immediately with the simple-to-use, high-resolution, color-display start menu</li><li>Exchange samples with ease with large, accessible sample compartment</li><li>Save your data fast any way you want it with multiple data-export options</li></ul><p>There is a GENESYS spectrophotometer you can count on to provide accurate, reproducible results for your application requirements.</p><h3><strong>GENESYS 50 UV-Vis Spectrophotometer</strong></h3><p><strong>For teaching labs, research labs and industrial QC requiring robust construction, ease-of-use and software capability.</strong></p><h5><strong>Advanced usability, modern functionality</strong></h5><ul><li>7-inch, high-resolution touchscreen interface</li><li>Local control increases speed and reliability for routine analysis</li><li>Large sample compartment with front access simplifies sample handling</li><li>Wi-Fi ready - save data and methods, print results from on-board control</li></ul><h5><strong>Designed for</strong></h5><ul><li>Chemistry labs</li><li>Routine measurements</li><li>Moderate throughput requirements</li></ul><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Pragolab_Thermo_Scientific_Genesys_50_Kyveta_bae9677ae0.jpg" alt="Thermo Scientific™ GENESYS™ 50 UV-Vis Spectrophotometer" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Pragolab_Thermo_Scientific_Genesys_50_Kyveta_bae9677ae0.jpg 188w," sizes="100vw" width="534" height="443"></p><h3><strong>Walk-up convenience</strong></h3><p><strong>Simple-to-use, touchscreen user interface features the methods and applications most important to you.</strong></p><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Thermo_Scientific_Genesys_spectrophotometers_ae282fe726.jpg" alt="Thermo Scientific GENESYS Spectrophotometers" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Thermo_Scientific_Genesys_spectrophotometers_ae282fe726.jpg 239w," sizes="100vw" width="1422" height="928"></p><h5><strong>Flexible computer control</strong></h5><p>Optional VISIONlite software offers complete control from a Microsoft ® Windows ® computer providing applications for:</p><ul><li>Scanning</li><li>Quantitative analysis</li><li>Kinetics</li><li>Fixed wavelength measurements</li></ul><h3><strong>GENESYS 50 UV-Vis Spectrophotometer Specifications</strong></h3><ul><li><strong>Baseline Flatness:</strong> ±0.002 A</li><li><strong>Compartments:</strong>&nbsp;<ul><li>Accessible from top, front or side</li><li>Able to accommodate cells up to 100 mm cells</li><li>Available accessory for test-tubes up to 25 mm diameter and 150 mm height</li><li>Removable, washable sample compartment liner with magnetic placement and hold-down</li></ul></li><li><strong>Connections:</strong>&nbsp;<ul><li>Single USB-A</li><li>Duplex USB-A</li><li>Export data to network or PC via USB, Ethernet, or Wi-Fi USB adaptor, available</li><li>Print via USB, Ethernet or Wi-Fi USB adaptor available</li></ul></li><li><strong>Dimensions (L x W x H):</strong> 35.5 x 38.5 x 19.5 cm</li><li><strong>Drift:</strong> (At 500 nm after 1 hour warmup) &lt;0.0005 A/hr.</li><li><strong>Electrical Requirements:</strong> External AC to DC converter. Voltage and Frequency (Hz) selected automatically, 100/240 V, 50/60 Hz</li><li><strong>Includes:</strong> GENESYS 50 Spectrophotometer</li><li><strong>Keypad:</strong> Touchscreen</li><li><strong>Lamp Life:</strong> &gt; 5 years typical, 3 years guaranteed</li><li><strong>Noise:</strong> (RMS at 500 nm 60 consecutive measurements)<ul><li>≤0.00020A at 0A at 260 and 500 nm</li><li>≤0.00030A at 1A at 260 and 500 nm</li><li>≤0.00040A at 2A at 260 and 500 nm</li></ul></li><li><strong>Optical Design:</strong> Dual Beam</li><li><strong>Photometric Accuracy:</strong> ±0.002A at 0.5A, ±0.004A at 1.0A, ±0.008 A at 2.0 A</li><li><strong>Photometric Display:</strong> -3A to +5A</li><li><strong>Photometric Range:</strong> –2A to +3.5A</li><li><strong>Photometric Repeatability:</strong> ±0.001 A at 1 A measured at 1.0 A at 546 nm</li><li><strong>Printer Included:</strong> Snap-on printer available</li><li><strong>Scan Speed:</strong> Slow, medium and fast – up to 1600 nm/min.</li><li><strong>Stray Light:</strong> &lt; 1.0%T 198 nm (KCl) , &lt;0.05%T at 220 nm (NaI), &lt;0.03%T at 340 nm (NaNO<sub>2</sub>)</li><li><strong>Warranty:</strong> 2 yrs std + 1 yr ext upon reg</li><li><strong>Wavelength Accuracy:</strong> ±0.5 nm</li><li><strong>Wavelength Data Interval:</strong> 0.2 nm, 0.5 nm, 1 nm, 2 nm, 5 nm</li><li><strong>Wavelength Repeatability:</strong> &lt; ±0.2 nm</li><li><strong>Weight (Metric):</strong> 7.5 kg</li><li><strong>Detector Type:</strong> Dual Silicon Photodiodes</li><li><strong>Display:</strong> 7 in. High Definition Color Touchscreen, Fixed, 800 x 1280 pixels</li><li><strong>Spectral Bandwidth:</strong> 2 nm</li><li><strong>Wavelength Range:</strong> 190 to 1100 nm</li><li><strong>Unit Size:</strong> Each</li></ul>

Thermo Scientific™ GENESYS™ 150 UV-Vis Spectrophotometer

Perform quantitative measurements in routine industrial QA/QC or university chemistry labs with the automated and network-capable Thermo Scientific™ GENESYS 150 UV-Vis Spectrophotometer.

<h4><strong>GENESYS - developed for users, designed for results</strong></h4><p>Built on the foundation of 60 years of spectroscopy experience, the next generation Thermo Scientific™ GENESYS™ family of Vis/UV-Vis spectrophotometers provides exceptional performance with a contemporary design.</p><ul><li>Start taking measurements immediately with the simple-to-use, high-resolution, color-display start menu</li><li>Exchange samples with ease with large, accessible sample compartment</li><li>Save your data fast any way you want it with multiple data-export options</li></ul><p>There is a GENESYS spectrophotometer you can count on to provide accurate, reproducible results for your application requirements.</p><h3><strong>GENESYS 150 UV-Vis Spectrophotometer</strong></h3><p><strong>For advanced teaching labs, R&amp;D, research and life science applications requiring higher throughput or temperature control.</strong></p><h5><strong>Elite performance, enhanced capabilities</strong></h5><ul><li>7-inch, high-resolution touchscreen interface</li><li>Local control increases speed and reliability for routine analysis</li><li>Large sample compartment with front access simplifies sample handling</li><li>Wi-Fi ready - save data and methods, print results from on-board control</li></ul><h5><strong>Designed for</strong></h5><ul><li>Chemistry labs</li><li>Routine measurements</li><li>Moderate throughput requirements</li></ul><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Pragolab_Thermo_Scientific_vis_spektrofotometr_genesys_150_titul_B_a202c02cea.jpg" alt="Thermo Scientific™ GENESYS™ 150 Uv-Vis Spectrophotometer" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Pragolab_Thermo_Scientific_vis_spektrofotometr_genesys_150_titul_B_a202c02cea.jpg 180w," sizes="100vw" width="415" height="360"></p><h3><strong>Walk-up convenience</strong></h3><p><strong>Simple-to-use, touchscreen user interface features the methods and applications most important to you.</strong></p><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Thermo_Scientific_Genesys_spectrophotometers_ae282fe726.jpg" alt="Thermo Scientific: Genesys spectrophotometers" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Thermo_Scientific_Genesys_spectrophotometers_ae282fe726.jpg 239w," sizes="100vw" width="1422" height="928"></p><h5><strong>Flexible computer control</strong></h5><p>Optional VISIONlite software offers complete control from a Microsoft ® Windows ® computer providing applications for:</p><ul><li>Scanning</li><li>Quantitative analysis</li><li>Kinetics</li><li>Fixed wavelength measurements</li></ul><h3><strong>GENESYS 150 UV-Vis Spectrophotometer Specifications</strong></h3><ul><li><strong>Baseline Flatness:</strong> ±0.002 A</li><li><strong>Compartments:</strong> Accommodates (optional):<ul><li>8-position cell changer</li><li>4-position cell changer (long path cells)</li><li>Peltier thermostatted cell holder (20–60 °C)</li><li>Sipper accessory</li><li>Fiber optic probe coupler</li></ul></li><li><strong>Connections:</strong>&nbsp;<ul><li>Single USB-A</li><li>Duplex USB-A</li><li>Export data to network or PC via USB, Ethernet, or Wi-Fi USB adaptor, available</li><li>Print via USB, Ethernet or Wi-Fi USB adaptor available</li></ul></li><li><strong>Dimensions (L x W x H):</strong> 35.5 x 38.5 x 19.5 cm</li><li><strong>Drift:</strong> (At 500 nm after 1 hour warmup) &lt;0.0005 A/hr.</li><li><strong>Electrical Requirements:</strong> External AC to DC converter. Voltage and Frequency (Hz) selected automatically, 100/240 V, 50/60 Hz</li><li><strong>Includes:</strong> Genesys 150 Spectrophotometer</li><li><strong>Keypad:</strong> Touchscreen</li><li><strong>Lamp Life:</strong> &gt; 5 years typical, 3 years guaranteed</li><li><strong>Noise:</strong> (RMS at 500 nm 60 consecutive measurements)<ul><li>≤0.00020A at 0A at 260 and 500 nm</li><li>≤0.00030A at 1A at 260 and 500 nm</li><li>≤0.00040A at 2A at 260 and 500 nm</li></ul></li><li><strong>Optical Design:</strong> Dual Beam</li><li><strong>Photometric Accuracy:</strong> ±0.002A at 0.5A, ±0.004A at 1.0A, ±0.008 A at 2.0 A</li><li><strong>Photometric Display:</strong> -3A to +5A</li><li><strong>Photometric Range:</strong> –2A to +3.5A</li><li><strong>Photometric Repeatability:</strong> ±0.001 A at 1 A measured at 1.0 A at 546 nm</li><li><strong>Printer Included:</strong> Snap-on printer available</li><li><strong>Scan Speed:</strong> Slow, medium and fast – up to 1600 nm/min.</li><li><strong>Stray Light:</strong> &lt; 1.0%T 198 nm (KCl) , &lt;0.05%T at 220 nm (NaI), &lt;0.03%T at 340 nm (NaNO<sub>2</sub>)</li><li><strong>Warranty:</strong> 2 yrs std + 1 yr ext upon reg</li><li><strong>Wavelength Accuracy:</strong> ±0.5 nm</li><li><strong>Wavelength Data Interval:</strong> 0.2 nm, 0.5 nm, 1 nm, 2 nm, 5 nm</li><li><strong>Wavelength Repeatability:</strong> &lt; ±0.2 nm</li><li><strong>Weight (Metric):</strong> 7.5 kg</li><li><strong>Detector Type:</strong> Dual Silicon Photodiodes</li><li><strong>Display:</strong> 7 in. High Definition Color Touchscreen, Tiltable, 800 x 1280 pixels</li><li><strong>Spectral Bandwidth:</strong> 2 nm</li><li><strong>Wavelength Range:</strong> 190 to 1100 nm</li><li><strong>Unit Size:</strong> Each</li></ul>

Thermo Scientific Orbitrap Exploris MX Mass Detector

Accelerate biopharmaceutical late-stage development, commercialization, manufacturing, and quality control (QC) by easily and reliably obtaining compliance-ready, high-quality mass information. 

<p><strong>Accelerate biopharmaceutical late-stage development, commercialization, manufacturing, and quality control (QC) by easily and reliably obtaining compliance-ready, high-quality mass information.&nbsp;</strong></p><p>The <strong>Thermo Scientific Orbitrap Exploris MX mass detector</strong> is the perfect fit for deploying routine mass monitoring.</p><p>In addition to high-resolution mass detection provided by Thermo Scientific Orbitrap mass analyzers, this fit for purpose system is simple to operate and compliance ready with <a target="_blank" rel="noopener noreferrer" href="https://www.thermofisher.com/cz/en/home/industrial/chromatography/chromatography-learning-center/chromeleon-cds-information/cds-mass-spectrometry-software.html">Thermo Scientific Chromeleon chromatography data system (CDS) software</a>. Consistent performance between the <a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/products/402">Thermo Scientific Orbitrap Exploris 240 mass spectrometer</a> and Orbitrap Exploris MX detector ensures seamless method transfer from development to QC.</p><h4><strong>High-resolution accurate-mass (HRAM) data provide utmost confidence for:</strong></h4><ul><li>Sequence confirmation</li><li>Peptide monitoring</li><li>Intact protein analysis (from small to large molecules, reduced mAbs, and intact mAbs under native and denaturing conditions)</li><li>Glycan profiling</li><li>Oligonucleotide and oligonucleotide impurity mass confirmation</li></ul><h3><strong>Highlights of Orbitrap Exploris MX mass detector&nbsp;</strong></h3><div colctrl-id="5-1"><div><div container-id="cq-colctrl-lt0" comp-id="A1"><div><h4><strong>Simplicity for scientists and technicians</strong></h4><p>Simplify tasks and reduce errors for more ‘right first time’ analyses with Chromeleon CDS, with built in methods, control, data processing, and management. The suite of smart tools makes your work faster and easier, while ensuring reproducible, high-quality mass monitoring.</p></div></div></div><div><div container-id="cq-colctrl-lt0" comp-id="B1"><div><h4><strong>Accelerate productivity</strong></h4><p>High uptime boosts productivity. Rapid “set and forget” calibration procedures provide consistent mass stability for at least four weeks at prescribed conditions. Instrument status monitoring and optimized pressure control alert users when maintenance is needed, avoiding unnecessary downtime and repeat analyses.</p></div></div></div></div><div colctrl-id="6-1"><div><div container-id="cq-colctrl-lt0" comp-id="A1"><div><h4><strong>Compliance for competitive advantage</strong></h4><p>Designed for data integrity, data security, and compliance, fully scalable Chromeleon CDS provides comprehensive technical controls to give you a competitive edge in meeting global regulatory requirements.</p></div></div></div><div><div container-id="cq-colctrl-lt0" comp-id="B1"><div><h4><strong>Consistent results deliver confidence</strong></h4><p>Robust and reliable performance from system to system and site to site ensure confidence in results used to make critical decisions. Industry-proven Orbitrap technology, now purposely designed for mass monitoring in future-proof lab operations.</p></div></div></div></div><div colctrl-id="7-1"><div><div container-id="cq-colctrl-lt0" comp-id="A1"><div><h4><strong>Seamless method transfer to MAM</strong></h4><p>Easy transfer of sophisticated Multi-Attribute Method (MAM) assays from development to manufacturing bridges the gap between development and QC.</p></div></div></div><div><div container-id="cq-colctrl-lt0" comp-id="B1"><div><h4><strong>Dedicated, global support team</strong></h4><p>The dedicated, global Thermo Scientific MAM team maximizes your productivity and confidence with installation, training, and fast service and support. Standardized system performance evaluation test (SET) and installation qualification/operational qualification (IQ/OA) thoroughly assess system performance with comprehensive installation and post-maintenance acceptance criteria.</p><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Thermo_Scientific_Orbitrap_Exploris_MX_Mass_Detector_4_2ad72d9b21.jpg" alt="Orbitrap Exploris MX mass stability: mass deviation relative to first observed mass in parts-per-million for 9 BSA tryptic peptides monitored over a period of 6 weeks using the new internal recalibration option. Each data point is an average of 19 individual injections." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Thermo_Scientific_Orbitrap_Exploris_MX_Mass_Detector_4_2ad72d9b21.jpg 184w," sizes="100vw" width="1240" height="1052"></p><h3><strong>Accelerate MAM deployment&nbsp;</strong></h3><h4><strong>Seamless knowledge and method transfer</strong></h4><p>Benefit from the end-to-end Thermo Scientific MAM 2.0 workflow approach to comprehensive characterization and monitoring of biotherapeutics from development to commercialization. <a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/products/402">Orbitrap Exploris 240</a> mass spectrometer methods, established during development, seamlessly transfer to the Orbitrap Exploris MX detector in manufacturing or by contract partners. In addition to full integration into the high-resolution MAM 2.0 workflow, the usability and low cost of the Orbitrap Exploris MX detector make it the perfect fit for high-throughput QC and contract labs.</p><p>Users can identify and evaluate relevant product quality attributes (PQAs) via high-throughput analytics provided by Thermo Scientific BioPharma Finder software. Connected labs can access data from development through QC using MAM 2.0 powered by Thermo Scientific Ardia Platform. And, PQAs can be monitored throughout the entire drug development process using the MAM target peptide workbook built from the list of identified peptides upon incorporation into a Chromeleon processing method. The processing method along with the instrument method, injection sequence, view setting and report template can be stored into a MAM eWorkflow for easy and direct transfer to QC via Chromeleon CDS Enterprise software.</p><div><h5><strong>MAM 2.0 offers:</strong></h5><ul><li>Fast decision making based on high-confidence PQAs obtained using industry-leading HRAM Orbitrap technology</li><li>Seamless knowledge and method transfer across instruments, departments, and sites using compliance-ready, Chromeleon CDS enterprise software, and Ardia Platform</li></ul><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Thermo_Scientific_Orbitrap_Exploris_MX_Mass_Detector_cd7474e7ed.jpg" alt="Thermo Scientific Orbitrap Exploris MX Mass Detector" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Thermo_Scientific_Orbitrap_Exploris_MX_Mass_Detector_cd7474e7ed.jpg 245w," sizes="100vw" width="1170" height="504"></p><blockquote><p><strong>Thermo Scientific MAM 2.0</strong></p><p>The end-to-end MAM solution for a fully connected, compliance-ready platform. MAM 2.0 includes everything you need to bring your next biotherapeutic candidate straight through to breakthrough.</p><ul><li><a target="_blank" rel="noopener noreferrer" href="https://www.thermofisher.com/cz/en/home/industrial/pharma-biopharma/biopharmaceutical-analytical-testing/multi-attribute-method.html"><strong>Learn more</strong></a></li></ul></blockquote></div></div></div></div></div>

Thermo Scientific Orbitrap Exploris Mass Spectrometers

Discover which Orbitrap Exploris mass spectrometry system will meet your business needs for more efficient screening, identification, confirmation and quantitation.

<p><strong>Benefit from record-setting performance. With built-in intelligence, sensitivity and selectivity; the Thermo Scientific Orbitrap Exploris mass spectrometry is quantitative high-resolution, accurate-mass (HRAM) liquid chromatography mass spectrometry (LC-MS) for analysis depth to the lowest levels with high quantitative accuracy and precision to meet your exact analytical requirements. Scale up your research and translate your results more rapidly and simply than ever before.</strong></p><h3><strong>Why choose an Orbitrap Exploris mass spectrometer for your laboratory?</strong></h3><p>Discover which Orbitrap Exploris mass spectrometry system will meet your business needs for more efficient screening (including targeted, suspected and nontargeted), identification, confirmation and quantitation.</p><h4><a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/products/1490"><strong>Orbitrap Exploris MX mass detector</strong></a></h4><p>Accelerate biopharmaceutical commercialization, manufacturing and quality control by easily and reliably obtaining compliance-ready, high-quality mass information with the Thermo Scientific Orbitrap Exploris MX Mass Detector.</p><p><strong>Typical applications: </strong>Biopharmaceutical characterization and QC, intact protein analysis, Multi-Attribute Method (MAM), oligonucleotides, pharmaceuticals</p><p><strong>Analyses: </strong>Targeted / non-targeted screening (MS), Unknown Identification (MS), Absolute and Relative Quantitation (MS)</p><p><strong>Resolution powers: </strong>180,000</p><p><strong>Mass range: </strong>m/z 40–3,000 up to m/z 8,000 with BioPharma option</p><p><strong>Scan speed: </strong>22Hz</p><p><strong>Sources &amp; Flexibility: </strong><a target="_blank" rel="noopener noreferrer" href="https://www.thermofisher.com/cz/en/home/industrial/mass-spectrometry/liquid-chromatography-mass-spectrometry-lc-ms/lc-ms-systems/orbitrap-lc-ms/orbitrap-exploris-mass-spectrometers.html#small">AP-SMALDI<sup>5</sup>&nbsp;AF source for MS Imaging</a></p><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Thermo_Scientific_Orbitrap_Exploris_MX_Mass_Detector_2_a84ed39672.jpg" alt="Thermo Scientific Orbitrap Exploris MX Mass Detector" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Thermo_Scientific_Orbitrap_Exploris_MX_Mass_Detector_2_a84ed39672.jpg 169w," sizes="100vw" width="1696" height="1575"></p><h4><a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/products/403"><strong>Orbitrap Exploris 120 Mass Spectrometer&nbsp;</strong></a></h4><p>Benefit from best in class high-resolution accurate mass for confident testing, screening and quantification of small molecules with the Thermo Scientific Orbitrap Exploris 120 Mass Spectrometer.&nbsp;&nbsp;</p><p><strong>Typical applications: </strong>Food and environmental testing, metabolomics, forensic toxicology, clinical research, pharmaceuticals</p><p><strong>Analyses: </strong>Targeted / non-targeted screening (MS), Unknown Identification (MS), Confirmation (MS/MS), Absolute and Relative Quantitation (MS and/or MS<sup>2</sup>)</p><p><strong>Resolution powers: </strong>120,000</p><p><strong>Mass range: </strong><i>m/z</i>&nbsp;40 – 3,000</p><p><strong>Scan speed: </strong>22Hz</p><p><strong>Sources &amp; Flexibility: </strong><a target="_blank" rel="noopener noreferrer" href="https://www.thermofisher.com/order/catalog/product/IQLAAEGABBFAGOMZZZ">APCI/APPI Combination</a>, <a target="_blank" rel="noopener noreferrer" href="https://www.thermofisher.com/cz/en/home/industrial/mass-spectrometry/liquid-chromatography-mass-spectrometry-lc-ms/lc-ms-systems/orbitrap-lc-ms/orbitrap-exploris-mass-spectrometers.html#small">AP-SMALDI<sup>5</sup>&nbsp;AF source for MS Imaging</a></p><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Thermo_Orbitrap_Exploris_120_Mass_Spectrometer_3_77e4e07555/Thermo-Orbitrap-Exploris-120-Mass-Spectrometer-3.jpg" alt="Thermo Orbitrap Exploris 120 Mass Spectrometer " srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Thermo_Orbitrap_Exploris_120_Mass_Spectrometer_3_77e4e07555/thumbnail_Thermo-Orbitrap-Exploris-120-Mass-Spectrometer-3.jpg 171w," sizes="100vw" width="938" height="856"></p><h4><a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/products/402"><strong>Orbitrap Exploris 240 Mass Spectrometer&nbsp;</strong></a></h4><p>Get unmatched confidence for discovery and identification, with application versatility and operational simplicity with the Thermo Scientific Orbitrap Exploris 240 Mass Spectrometer.&nbsp;</p><p><strong>Typical applications: </strong>Biopharmaceutical characterization, proteomics, metabolomics, lipidomics, food and environmental research, clinical research</p><p><strong>Analyses: </strong>Targeted / non-targeted screening (MS), Unknown Identification (MS), Confirmation (MS/MS), Absolute and Relative Quantitation (MS and/or MS<sup>2</sup>)</p><p><strong>Resolution powers: </strong>240,000</p><p><strong>Mass range: </strong><i>m/z</i>&nbsp;40 - 6000, (up to 8000 with BioPharma option)</p><p><strong>Scan speed: </strong>30Hz</p><p><strong>Sources &amp; Flexibility: </strong><a target="_blank" rel="noopener noreferrer" href="https://www.thermofisher.com/us/en/home/industrial/mass-spectrometry/liquid-chromatography-mass-spectrometry-lc-ms/lc-ms-accessories/field-asymmetric-ion-mobility-spectrometry-faims.html">FAIMS Pro Duo interface</a>, <a target="_blank" rel="noopener noreferrer" href="https://www.thermofisher.com/order/catalog/product/IQLAAEGABBFAGOMZZZ">APCI/APPI Combination</a>, <a target="_blank" rel="noopener noreferrer" href="https://www.thermofisher.com/order/catalog/product/ES081">EASY-Spray Source</a>, <a target="_blank" rel="noopener noreferrer" href="https://www.thermofisher.com/order/catalog/product/ES071">Nanospray Flex Ion Sources</a>&nbsp;, <a target="_blank" rel="noopener noreferrer" href="https://www.thermofisher.com/cz/en/home/industrial/mass-spectrometry/liquid-chromatography-mass-spectrometry-lc-ms/lc-ms-systems/orbitrap-lc-ms/orbitrap-exploris-mass-spectrometers.html#small">AP-SMALDI<sup>5</sup>&nbsp;AF source for MS Imaging</a></p><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Thermo_Orbitrap_Exploris_240_Mass_Spectrometer_2_a5085c34b0/Thermo-Orbitrap-Exploris-240-Mass-Spectrometer-2.jpg" alt="Thermo Orbitrap Exploris 240 Mass Spectrometer " srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Thermo_Orbitrap_Exploris_240_Mass_Spectrometer_2_a5085c34b0/thumbnail_Thermo-Orbitrap-Exploris-240-Mass-Spectrometer-2.jpg 175w," sizes="100vw" width="945" height="841"></p><h4><a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/products/401"><strong>Orbitrap Exploris 480 Mass Spectrometer&nbsp;</strong></a></h4><p>Achieve next-level insights, exploratory and targeted quantitation, next-gen power, performance, and ease of use with the Thermo Scientific Orbitrap Exploris 480 Mass Spectrometer.</p><p><strong>Typical applications: </strong>Shotgun proteomics (LFQ DIA, DDA), single cell proteomics, TMT multiplexing, targeted peptide quantification, metabolomics, biopharmaceutical applications (including HCP and protein characterization).</p><p><strong>Analyses: </strong>Targeted / non-targeted screening (MS), Unknown Identification (MS), Confirmation (MS/MS), Absolute and Relative Quantitation (MS and/or MS<sup>2</sup>)</p><p><strong>Resolution powers: </strong>480,000</p><p><strong>Mass range: </strong><i>m/z</i>&nbsp;40 - 6000, (up to 8000 with BioPharma option)</p><p><strong>Scan speed: </strong>40Hz</p><p><strong>Sources &amp; Flexibility: </strong><a target="_blank" rel="noopener noreferrer" href="https://www.thermofisher.com/us/en/home/industrial/mass-spectrometry/liquid-chromatography-mass-spectrometry-lc-ms/lc-ms-accessories/field-asymmetric-ion-mobility-spectrometry-faims.html">FAIMS Pro Duo interface</a>, <a target="_blank" rel="noopener noreferrer" href="https://www.thermofisher.com/order/catalog/product/IQLAAEGABBFAGOMZZZ">APCI/APPI Combination</a>, <a target="_blank" rel="noopener noreferrer" href="https://www.thermofisher.com/order/catalog/product/ES081">EASY-Spray Source</a>, <a target="_blank" rel="noopener noreferrer" href="https://www.thermofisher.com/order/catalog/product/ES071">Nanospray Flex Ion Sources</a>&nbsp;, <a target="_blank" rel="noopener noreferrer" href="https://www.thermofisher.com/cz/en/home/industrial/mass-spectrometry/liquid-chromatography-mass-spectrometry-lc-ms/lc-ms-systems/orbitrap-lc-ms/orbitrap-exploris-mass-spectrometers.html#small">AP-SMALDI<sup>5</sup>&nbsp;AF source for MS Imaging</a></p><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Thermo_Orbitrap_Exploris_480_Mass_Spectrometer_2_3d91c9b5b1/Thermo-Orbitrap-Exploris-480-Mass-Spectrometer-2.jpg" alt="Thermo Orbitrap Exploris 480 Mass Spectrometer " srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Thermo_Orbitrap_Exploris_480_Mass_Spectrometer_2_3d91c9b5b1/thumbnail_Thermo-Orbitrap-Exploris-480-Mass-Spectrometer-2.jpg 235w," sizes="100vw" width="880" height="584"></p>

Thermo Scientific™ Dionex™ Inuvion™ Core ion chromatography system

The Inuvion Core system gives you all the benefits of chemical suppression and higher-pressure operation, with manual eluent preparation.

<h3><strong>Welcome to the evolution of everyday IC reliability</strong></h3><p>The new<strong> Thermo Scientific™ Dionex™ Inuvion™ ion chromatography system</strong> makes ion analysis simpler and more intuitive than ever before. And it delivers exactly what a busy analytical testing lab is looking for: <strong>consistently excellent results-every shift, every day, for every operator.</strong></p><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Thermo_Scientific_Dionex_Inuvion_ion_chromatography_system_2_6400708696.jpg" alt="Thermo Scientific™ Dionex™ Inuvion™ ion chromatography system." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Thermo_Scientific_Dionex_Inuvion_ion_chromatography_system_2_6400708696.jpg 245w," sizes="100vw" width="825" height="498"></p><h3><strong>Start with Inuvion Core</strong></h3><p>The <strong>Inuvion Core system</strong> gives you<strong> </strong>all the benefits of chemical suppression and higher-pressure operation, with manual eluent preparation. You still enjoy the<strong> same robust Dionex Inuvion platform</strong>-easily configurable and economically upgradeable to full-featured capabilities as budget allows.</p><figure class="image"><img style="aspect-ratio:650/650;" src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Thermo_Scientific_Dionex_Inuvion_Core_ion_chromatography_system_6c8824cb1d.jpg" alt="Thermo Scientific™ Dionex™ Inuvion™ Core ion chromatography system." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Thermo_Scientific_Dionex_Inuvion_Core_ion_chromatography_system_6c8824cb1d.jpg 156w," sizes="100vw" width="650" height="650"></figure><h3><strong>SIMPLE, INTUITIVE USER EXPERIENCE&nbsp;</strong></h3><ul><li><strong>Smart, function-driven design</strong> lets you quickly and safely access everything on the instrument—no hard-to-reach compartments, and no step stools or extra tools needed!</li><li><strong>Automated startup and shutdown routines</strong> ensure the system is quickly ready for the day’s work without user intervention.&nbsp;</li><li><strong>Built-in how-to videos</strong> reduce training time and simplify setup and operation.</li></ul><h3><strong>ULTRA-RELIABLE PERFORMANCE&nbsp;</strong></h3><ul><li><strong>Advanced high-performance pump technology and electronics</strong> let you take advantage of a wider range of columns and chemistries to improve the speed and quality of your results.</li><li><strong>Fast, flexible, convenient electrolytic suppression: </strong>Just connect the suppressor; no external pump or offline conditioning needed for regeneration.</li><li><strong>Reagent-free IC (RFIC™) capability</strong> makes eluent preparation as simple as “just add water,” while ensuring more consistent results and enabling optimized gradient separations.</li></ul><h3><strong>EASILY CONFIGURABLE AND UPGRADEABLE</strong></h3><ul><li><strong>Versatile, adaptable platform</strong> lets you configure the exact Dionex Inuvion IC system for today’s needs and budget, with confidence that your system will also meet your future needs.</li><li><strong>Multiple options and peripherals </strong>extend IC capabilities, letting you easily—and cost-effectively—adapt to changing sample types and workflow requirements.</li></ul><h3><strong>Simply the best system for everyday ion analysis</strong></h3><figure class="image"><img style="aspect-ratio:1107/755;" src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Thermo_Scientific_Dionex_Inuvion_ion_chromatography_system_d855feb2a8.jpg" alt="Thermo Scientific™ Dionex™ Inuvion™ ion chromatography system." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Thermo_Scientific_Dionex_Inuvion_ion_chromatography_system_d855feb2a8.jpg 229w," sizes="100vw" width="1107" height="755"></figure><p><strong>1. Operator-friendly engineering </strong>saves time and reduces errors by allowing easy, unobstructed access to all components, connections and consumables</p><p><strong>2. Compact design </strong>helps you make the most of valuable bench space</p><p><strong>3. Digital gas pressure regulator </strong>increases precision of gas delivery for maximum day-to-day consistency (optional)</p><p><strong>4. Temperature-controlled conductivity cell </strong>improves reproducibility and ensures baseline stability</p><p><strong>5. Vacuum degassing capability</strong> enables more consistent peak identification from smoother baselines</p><p><strong>6. Seal wash pump</strong> reduces maintenance costs when using manually prepared eluents (optional)&nbsp;</p><p><strong>7. Reagent-free IC (RFIC)</strong> saves time and ensures greater day-to-day and operator-to-operator consistency, while also allowing the use of gradient separations</p><p><strong>8. Electrolytic suppression</strong> entirely eliminates the need for manual regenerant preparation (or, opt for a continuously regenerated, always- ready-to-go chemical suppressor)</p><p><strong>9. Thermo Scientific™ Dionex™ IC PEEK Viper™ fittings</strong> ensure confident, low dead volume connections every time—no tools required! (optional)</p><p><strong>10. Integrated column heater</strong> minimizes the impact of fluctuations in lab temperature that can adversely affect results (optional)</p><p><strong>11. 6- or 10-port auxiliary valve</strong> lets you automate in-line sample preparation</p><p><strong>12. State-of-the-art high-pressure pump (up to 5000 psi)</strong> lets you increase flow rates with smaller particle columns to reduce run times or add additional separation capacity with longer column length</p><p><strong>13. System self-diagnostics </strong>automatically detect any issues with hardware and consumables</p><p><strong>14. Self-service capabilities and user-swappable parts</strong> help you get back up and running in a hurry Automatic eluent monitor alerts the operator to low solution levels, avoiding potential system damage or failed runs (optional)</p><p><strong>15. Automatic eluent monitor </strong>alerts the operator to low solution levels, avoiding potential system damage or failed runs (optional)</p><h3><strong>Flexible, adaptable Dionex Inuvion platform</strong></h3><p>You can <strong>configure your Dionex Inuvion system</strong> to meet specific analytical and budget requirements, and easily reconfigure as needed. Multiple configurations and peripherals extend IC capabilities, letting you easily-and cost-effectively-adapt to changing sample types and workflow requirements.</p><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Thermo_Scientific_Dionex_Inuvion_ion_chromatography_system_3_fa8233bcd4.jpg" alt="Thermo Scientific™ Dionex™ Inuvion™ ion chromatography system." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Thermo_Scientific_Dionex_Inuvion_ion_chromatography_system_3_fa8233bcd4.jpg 245w," sizes="100vw" width="1246" height="572"></p><h3><strong>Consistently excellent results every day</strong></h3><p>The <strong>Dionex Inuvion IC system supports a full range of columns </strong>to improve the speed and quality of results, for both<strong> anions and cations.</strong> Expanded capabilities, such as Reagent-Free IC (RFIC), enhance operator-to-operator consistency, while enabling the use of eluent gradients to optimize separations.</p><h4><strong>Rapid results</strong></h4><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Pragolab_Obr_2_Ukazka_rychle_separace_zakladnich_aniontu_s_uhlicitanovou_MF_Zkraceni_doby_analyzy_diky_typu_kolony_a_vyssimu_prutoku_0e610b919d.jpg" alt="Thermo Scientific™ Dionex™ Inuvion™ Core ion chromatography system: Figure 1. Elution of seven common inorganic anions using carbonate eluent. Short columns and fast flow rates let you reduce run times." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Pragolab_Obr_2_Ukazka_rychle_separace_zakladnich_aniontu_s_uhlicitanovou_MF_Zkraceni_doby_analyzy_diky_typu_kolony_a_vyssimu_prutoku_0e610b919d.jpg 245w," sizes="100vw" width="1622" height="739"></p><h4><strong>Exceptional reproducibility</strong></h4><figure class="image"><img style="aspect-ratio:1693/754;" src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Pragolab_Obr_3_Ukazka_reprodukovatelnosti_analyzy_zakladnich_aniontu_40_nastriku_Spickova_technologie_cerpadla_a_elektronika_pristroje_poskytuji_konzistentni_a_reprodukovatelne_vysledky_f66e7430cf.jpg" alt="Thermo Scientific™ Dionex™ Inuvion™ Core ion chromatography system: Figure 2. Overlay of 40 replicate injections. State-of-the-art pump technology and Electronics deliver consistent, reproducible results." width="1693" height="754"></figure><h4><strong>Increased sensitivity and consistency</strong></h4><figure class="image"><img style="aspect-ratio:1860/836;" src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Pragolab_Obr_4_Ukazka_vysoce_citlive_analyzy_aniontu_ve_vodach_Eluent_generator_KOH_a_elektrolyticka_suprese_snizuji_pozadi_cimz_dochazi_ke_zvyseni_citlivosti_da55facfd0.jpg" alt="Thermo Scientific™ Dionex™ Inuvion™ Core ion chromatography system: Figure 3. Determination of inorganic anions in drinking and surface waters. KOH eluent generation and electrolytic suppression yield lower backgrounds for greater sensitivity and increased day-to-day consistency." width="1860" height="836"></figure><h4><strong>Method optimization</strong></h4><figure class="image"><img style="aspect-ratio:1799/889;" src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Pragolab_Obr_5_Ukazka_optimalizace_metody_zkraceni_doby_analyzy_pouzitim_gradientu_mobilni_faze_fc1fbe9cb0.jpg" alt="Thermo Scientific™ Dionex™ Inuvion™ Core ion chromatography system: Figure 4. Comparison of isocratic and gradient runs using eluent generation. Reduce run length and sharpen later-eluting peaks with the same ease as an isocratic run with no need for two separate eluents or a proportioning pump." width="1799" height="889"></figure><h3><strong>Compliance-ready Chromeleon CDS</strong></h3><p>A trusted industry standard all over the world, ”Simply Intelligent”<strong> </strong><a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/products/76"><strong>Thermo Scientific™ Chromeleon™ Chromatography Data System (CDS) software </strong></a>brings out the full power of the Dionex Inuvion IC system. Easy to learn and easy to use, it automates and optimizes daily tasks and helps even inexperienced users achieve consistent, high quality results.</p><p><img style="aspect-ratio:3700/1660;" src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Thermo_Scientific_Thermo_Scientific_Dionex_Inuvion_2_303a6eb73b.jpg" alt="Thermo Scientific™ Dionex™ Inuvion™ Core ion chromatography system." width="3700" height="1660"></p>

Thermo Scientific™ LipidSearch™ Software

Thermo Scientific™ LipidSearch™ software provides accurate identification of lipids and automatically integrates complex data into a concise report.

<h3><strong>The promise of lipidomics</strong></h3><p><strong>Lipidomics is a new field of study crucial for understanding cellular physiology and pathology. The application of lipidomic profiling to disease phenotype analysis is a rapidly growing aspect of translational medical research. Identification of unique lipid biomarkers has the potential to distinguish healthy individuals from individuals at risk for disease, detect diseases earlier, and facilitate development of personalized treatments.</strong></p><p>Liquid chromatography combined with mass spectrometry (LC/MS) is a widely adopted technique for lipidomics analyses. Relative and absolute quantitation, and identification, of lipids from biological samples requires sophisticated software with an extensive, comprehensive database.<strong> Thermo Scientific™ LipidSearch™ software provides accurate identification of lipids and automatically integrates complex data into a concise report. With its easy-to-use web-based interface, it dramatically reduces data analysis time.</strong></p><figure class="image"><img style="aspect-ratio:915/494;" src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Thermo_Scientific_Lipid_Search_Software_2_73b7d0a9e2.jpg" alt="Thermo Scientific™ LipidSearch™ Software: Easy-to-use web browser interface for automated and reliable identification." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Thermo_Scientific_Lipid_Search_Software_2_73b7d0a9e2.jpg 245w," sizes="100vw" width="915" height="494"></figure><h3><strong>Automated identification and relative quantitation with LipidSearch software</strong></h3><p>LipidSearch software, developed jointly by Professor Ryo Taguchi and MKI, (Tokyo, Japan), is a powerful new tool for<strong> automatic identification and relative quantification of cellular lipid molecular species from large amounts of mass spectrometric data obtained in nano-infusion or LC-MS experiments. </strong>Using the industry-leading, high-resolution, accurate-mass <a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/products?category=5&amp;manufacturerItems=3&amp;search=orbitrap"><strong>Thermo Scientific Orbitrap™ technology</strong></a> with exclusive<strong> LipidSearch software</strong>, the most accurate and confident lipid profiles and identifications can be achieved more quickly than ever before.</p><ul><li><strong>Compatible with</strong> data acquired from Thermo Scientific™ triple quadrupole, ion trap, and Orbitrap mass spectrometers</li><li><strong>Largest lipid database</strong> containing <strong>&gt;1.5 million</strong> lipid ions and their predicted fragment ions</li><li><strong>Identification algorithms</strong> for product ion, precursor ion and neutral loss scans</li><li><strong>Alignment of lipid data</strong> obtained from multiple<strong> LC-MS and MS<sup>n</sup> experiments</strong></li><li><strong>Relative quantitation </strong>of identified lipid precursors in either LC-MS or infusion experiments</li></ul><p>The database contains <strong>defined structures and includes more than 1.5 million lipid ions and their predicted fragment ions. </strong>Fragmentation patterns are calculated and improved by using expert knowledge based on experimental results. Lipid adduct ions and MS<sup>n</sup> fingerprints are also included. Data are stored in XML files and are easily customized.</p><figure class="image"><img style="aspect-ratio:990/497;" src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Thermo_Scientific_Lipid_Search_Software_3_91f1458663.jpg" alt="Thermo Scientific™ LipidSearch™ Software: Lipid fragment ions related to lipid headgroup, fatty acids, and backbone." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Thermo_Scientific_Lipid_Search_Software_3_91f1458663.jpg 245w," sizes="100vw" width="990" height="497"></figure><h3><strong>LipidSearch software provides an easy-to-use, automated workflow</strong></h3><p><strong>From peak detection to relative quantitaiton and identification, LipidSearch software provides an easy-to-use, automated workflow.</strong></p><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Thermo_Scientific_Lipid_Search_Software_4_2043f3d667.jpg" alt="Thermo Scientific™ LipidSearch™ Software: LipidSearch software provides an easy-to-use, automated workflow." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Thermo_Scientific_Lipid_Search_Software_4_2043f3d667.jpg 245w," sizes="100vw" width="1122" height="530"></p><h4><strong>Step 1 Data Analysis Module — Peak Detection</strong></h4><ul><li><strong>Raw data file reading</strong></li><li><strong>Smart peak detection</strong></li></ul><p>The peak detection engine implemented in<strong> LipidSearch software </strong>can handle<strong> different MS experiments and platforms. </strong>The combination of unique peak detection algorithms, tailored for each experiment and instrument type, and mass spectral processing functions enables accurate peak detection.</p><p><strong>LipidSearch software provides two different identification algorithms as well as scoring algorithms:</strong></p><ul><li><strong>A group-specific algorithm</strong> identifies lipids based on the polar head groups or fatty acids using a combination of precursor ion scanning and neutral loss scans from lipids mixtures.</li><li><strong>The comprehensive ID algorithm</strong> is used for product ion scans and can discriminate each lipid by matching the predicted fragmentation pattern stored in the database.</li><li>LipidSearch software also provides a <strong>set of scoring algorithms</strong> to filter out lower probability results</li></ul><h4><strong>Step 2 Identification Module — Lipid Identification</strong></h4><ul><li><strong>Group-specific ID (targeted)</strong></li><li><strong>Comprehensive ID (untargeted)</strong></li><li><strong>Scoring algorithms</strong></li></ul><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Thermo_Scientific_Lipid_Search_Software_5_0abd534dd5.jpg" alt="Thermo Scientific™ LipidSearch™ Software: PG 17:0/17:0 Search Results." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Thermo_Scientific_Lipid_Search_Software_5_0abd534dd5.jpg 245w," sizes="100vw" width="1003" height="456"></p><h4><strong>Step 3 Quantitation Module — Alignment and Quantitation</strong></h4><ul><li><strong>XIC peak integration</strong></li><li><strong>Retention time alignment</strong></li><li><strong>Relative quantitation</strong></li><li><strong>Statistical analysis</strong></li></ul><p>Prior to quantitation, lipid results from each sample are aligned within a retention time window. Identified lipids are quantified by detecting their precursor ions from full-scan MS and integrating extracted ion chromatograms.</p><p>Accurate peak areas are computed by denoising and smoothing the peak profiles prior to separating any partially overlapped peaks. Comparative analysis is then carried out between the multiple sample and control groups using <strong>t-test statistics.</strong> The mean peak area result for each group is displayed in a “box and whisker” plot.</p><figure class="image"><img style="aspect-ratio:1129/909;" src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Thermo_Scientific_Lipid_Search_Software_6_196073034c.jpg" alt="Thermo Scientific™ LipidSearch™ Software: Alignment results for CoQ7, [M+NH4]+." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Thermo_Scientific_Lipid_Search_Software_6_196073034c.jpg 194w," sizes="100vw" width="1129" height="909"></figure><p>Using the industry-leading, high-resolution, accurate-mass <a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/products?category=5&amp;manufacturerItems=3&amp;search=orbitrap"><strong>Thermo Scientific™ Orbitrap™ technology</strong></a><strong> </strong>with exclusive<strong> LipidSearch software</strong>, achieve the most accurate and confident lipid profiles and identifications more quickly than ever before. For targeted quantitation, LipidSearch software also supports the <a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/products?category=5&amp;manufacturerItems=3&amp;search=tsq"><strong>Thermo Scientific TSQ triple quadrupole MS systems</strong></a><strong>. </strong>For both lipidomics workflows, LipidSearch software <strong>automatically integrates complex data into a combined report to dramatically reduce data analysis.</strong></p><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Thermo_Scientific_Lipid_Search_Software_7_f6a5102f0a.jpg" alt="Thermo Scientific™ LipidSearch™ Software." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Thermo_Scientific_Lipid_Search_Software_7_f6a5102f0a.jpg 245w," sizes="100vw" width="924" height="570"></p><h5><strong>Minimum/Recommended PC Requirements</strong></h5><ul><li><strong>OS: </strong>Microsoft® Windows® 7 Professional (x64) or Windows 8 (x64); English language<br><strong>CPU: </strong>Quad - or multi-core CPU, 2 GHz or higher<br><strong>Memory:</strong> 8 GB RAM or higher<br><strong>HDD: </strong>50 GB free space for LipidSearch software; 500 GB hard drive<br>SSD (optional): 50 GB free space for LipidSearch software; 256 GB solid-state drive</li></ul>

Thermo Scientific™ ProSightPD Software

Perform comprehensive analysis of top-down proteomics data with Thermo Scientific™ ProSightPD™ software.

<div container-id="cq-colctrl-lt8" comp-id="B1"><div><h3><strong>Confident proteoform identification and characterization</strong></h3></div></div><div container-id="cq-colctrl-lt8" comp-id="B2"><div><p><strong>Uncover and confidently characterize proteoforms within complex matrices, including proteoforms containing truncations, alternative splices, and post-translationally modified proteins.</strong>&nbsp;</p><p>Extensive searches make use of <strong>various fragmentation methodologies (e.g., ETD, HCD, UVPD)</strong>, allowing you to make full use of the high-quality <strong>MS/MS data</strong> obtained when searching against the<strong> full biological complexity present in UniProt. </strong>With<strong> Thermo Scientific ProSightPD software, </strong>see what is really there and make the most of your data.</p><div><h3><strong>Key benefits</strong></h3><h5><strong>Sophisticated tools at your fingertips</strong></h5><p><strong>Utilize several search modes</strong> for identifying intact and truncated proteins. ProSightPD software offers the ability to search entire raw files or quickly select specific MS<sup>2</sup> scans for further searching. Information in UniProt enables the identification of modified or processed proteins.</p><h5><strong>Remove the guesswork from database management</strong></h5><p><strong>Import result files </strong>from<a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/products/1782"><strong> Proteome Discoverer</strong></a><strong> software</strong> or entire proteomes via UniProt formatted XML and FASTA files with Database Manager. Fully customize imported databases using thr<strong> ProSight Annotator GUI </strong>to characterize PTMs, disulfide bonds, mutations and truncations.</p><h5><strong>Intuitive user interface</strong></h5><p><strong>Analyze large datasets</strong> with many data files using <strong>ProSightPD nodes for </strong><a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/products/1782"><strong>Proteome Discoverer</strong></a><strong> software. </strong>Create multi-step searches and utilize built in results interpretation tools.</p><h3><strong>Search Types</strong></h3><div><p><strong>Different database search modes</strong> allow you to optimize data interrogation based upon your experimental needs. Searches are driven through the use of a node-based system allowing flexibility of experimental design.</p><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Thermo_Scientific_Pro_Sight_PD_Software_8a96430534.jpeg" alt="Thermo Scientific™ ProSightPD Software: ProSightPD Database Searching." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Thermo_Scientific_Pro_Sight_PD_Software_8a96430534.jpeg 245w," sizes="100vw" width="960" height="540"></p><div><p>Further information relating to the two search modes, Absolute Mass Search and Biomarker Search.</p><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Thermo_Scientific_Pro_Sight_PD_Software_2_01f6c31076.jpeg" alt="Thermo Scientific™ ProSightPD Software: Complementary Search Types: Digging Deeper in Complex Samples." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Thermo_Scientific_Pro_Sight_PD_Software_2_01f6c31076.jpeg 245w," sizes="100vw" width="960" height="540"></p><h3><strong>Database Management</strong></h3><div><p><strong>Confident proteoform identification </strong>is realized through the use of XML-formatted databases, ensuring that nothing is missed.</p><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Thermo_Scientific_Pro_Sight_PD_Software_3_db19f0cee9.jpeg" alt="Thermo Scientific™ ProSightPD Software: Database Manager: Add remove isoforms and PTMs." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Thermo_Scientific_Pro_Sight_PD_Software_3_db19f0cee9.jpeg 245w," sizes="100vw" width="960" height="540"></p><div><p><strong>Improvements to data visualization</strong> ensure that you can better interact with and interpret your data, minimizing the time taken to deliver actionable results.</p><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Thermo_Scientific_Pro_Sight_PD_Software_4_575aa9dff5.jpeg" alt="Thermo Scientific™ ProSightPD Software: LFQ Visualizations." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Thermo_Scientific_Pro_Sight_PD_Software_4_575aa9dff5.jpeg 245w," sizes="100vw" width="960" height="540"></p><h3><strong>ProSightPD nodes</strong></h3><p><strong>Top-Down Workflows:</strong> ProSightPD&nbsp;includes templates to easily build the most commonly used-top down data analysis workflows.</p><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Thermo_Scientific_Pro_Sight_PD_Software_5_4f28a7434d.jpg" alt="Thermo Scientific™ ProSightPD Software: ProSightPD 2.0: Tailored Workflows for All Types of Data." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Thermo_Scientific_Pro_Sight_PD_Software_5_4f28a7434d.jpg 245w," sizes="100vw" width="740" height="400"></p></div></div></div></div></div></div></div>

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