🎤Dr. Leigh Terry (University of Alabama)In this episode of Concentrating on Chromatography, we sit down with Dr. Leigh Terry from the University of Alabama to explore one of the biggest challenges in environmental analysis today: understanding—and accurately measuring—PFAS in water systems.While most regulatory methods focus on a small number of compounds like PFOA and PFOS, there may be thousands of PFAS compounds in the environment—and much of that burden goes undetected.<h4>In this episode, we cover:</h4><ul><li>Why PFAS are called “forever chemicals” (and why that label is evolving)</li><li>The limitations of targeted LC-MS/MS methods</li><li>What the fluorine mass balance problem reveals about PFAS destruction</li><li>How combustion ion chromatography (CIC) helps measure total organofluorine</li><li>Why “99.99% removal” doesn’t always mean what you think</li><li>Where PFAS actually end up in wastewater treatment systems</li><li>The most effective technologies for PFAS removal today</li><li>Why drinking water may only account for ~20% of total PFAS exposure</li></ul><h4>Key Insight:</h4>Even when analyzing dozens of PFAS compounds, researchers may only be capturing a small fraction of total organic fluorine—raising major questions about how we monitor and regulate these contaminants.<h3>Video Transcription</h3>In this episode of Concentrating on Chromatography, Dr. Leigh Terry discusses PFAS contamination in drinking water, the analytical challenges associated with measuring these compounds, current treatment technologies, and future directions for monitoring and regulation. The conversation highlights how analytical chemistry, environmental engineering, and public health intersect in addressing one of today's most discussed classes of emerging contaminants.<h4>Understanding PFAS and Their Significance</h4><h5>The University of Alabama Water Quality Research Center</h5>Dr. Leigh Terry is part of the University of Alabama's Center for Water Quality Research. The center operates an analytical laboratory focused on drinking water quality and the detection of contaminants at extremely low concentrations.The laboratory specializes in:<ul><li>Drinking water analysis</li><li>Organic contaminants</li><li>Emerging contaminants</li><li>PFAS monitoring</li><li>Water quality research</li></ul>Their work supports both research initiatives and practical solutions for water utilities and environmental agencies.<h4>Why Focus on PFAS?</h4>Although PFAS compounds have existed since the 1940s, public awareness has increased dramatically only in recent years.According to Dr. Terry, several factors contributed to the rapid growth of PFAS research:<ul><li>Increased media attention</li><li>New regulatory initiatives</li><li>Growing evidence of human exposure</li><li>Expanding research opportunities</li><li>Greater understanding of potential health risks</li></ul>PFAS are not new contaminants. Rather, society is only beginning to understand their prevalence in the environment and their potential impacts on human health.<h4>The Development of Standardized PFAS Methods</h4>One major advancement in PFAS analysis has been the development of standardized EPA methods.These methods allow:<ul><li>Consistent testing procedures</li><li>Comparable results between laboratories</li><li>Regulatory monitoring</li><li>Reliable data interpretation</li></ul>Dr. Terry notes that many of these standardized approaches began appearing around 2018–2019, providing a common framework for analytical laboratories and researchers.<h4>How Large Is the PFAS Universe?</h4>Many people are familiar with PFOA and PFOS, two of the most heavily studied PFAS compounds. However, these represent only a small fraction of the broader PFAS family.PFOA and PFOS are often referred to as:<ul><li>Legacy PFAS compounds</li><li>Original PFAS contaminants</li><li>Early commercial PFAS chemicals</li></ul>The challenge is that even minor modifications to molecular structure create entirely new PFAS compounds.Researchers estimate:<ul><li>Thousands of PFAS compounds exist</li><li>Some estimates exceed 10,000 compounds</li><li>Exact numbers depend on how PFAS are defined</li></ul>Scientific debate continues regarding classification and definition, making precise counting difficult.<h4>Why PFAS Are Called "Forever Chemicals"</h4>Dr. Terry notes that the phrase "forever chemicals" has become widely used, although she suggests the terminology could benefit from rebranding.PFAS persistence results from two key chemical properties.<h5>1. Amphiphilic Structure</h5>PFAS molecules contain:<ul><li>A water-loving (hydrophilic) head group</li><li>A water-repelling (hydrophobic) fluorinated tail</li></ul>This unusual combination allows PFAS to partition between:<ul><li>Water</li><li>Solids</li><li>Air-water interfaces</li><li>Foam</li></ul>Unlike many organic compounds, PFAS can interact effectively with multiple environmental phases.<h5>2. Extremely Strong Carbon–Fluorine Bonds</h5>The carbon–fluorine bond is among the strongest bonds in organic chemistry.As Dr. Terry explains:<ul><li>Large amounts of energy are required to break these bonds</li><li>Natural degradation processes are often ineffective</li><li>Destruction can require highly aggressive treatment conditions</li></ul>These properties make PFAS extremely persistent in both environmental systems and biological organisms.<h4>Toxicity, Regulations, and Environmental Behavior</h4><h5>Understanding PFAS Toxicity</h5>One of the biggest challenges in PFAS regulation is the incomplete understanding of toxicity.Dr. Terry explains that EPA regulations generally consider three factors:<ul><li>Presence in drinking water sources</li><li>Toxicity to humans</li><li>Availability of practical treatment options</li></ul>While some PFAS compounds are known to be harmful, many others remain insufficiently studied. Furthermore, PFAS often occur as mixtures rather than individual compounds.This creates important scientific questions:<ul><li>What is the toxicity of individual PFAS?</li><li>What is the toxicity of PFAS mixtures?</li><li>How should cumulative exposure be evaluated?</li></ul>These uncertainties continue to drive active research.<h4>What Happens to PFAS in Wastewater Treatment Plants?</h4>PFAS do not behave like many conventional contaminants.Rather than remaining fully dissolved in water, they often accumulate at interfaces such as:<ul><li>Water-solid interfaces</li><li>Water-air interfaces</li><li>Foam layers</li><li>Biological solids</li></ul>As a result:<ul><li>Some PFAS accumulate in biosolids</li><li>Some partition into foam</li><li>Some remain in treated effluent</li></ul>Their distribution depends on wastewater composition and treatment processes.<h4>Regulatory Monitoring and Targeted LC-MS Analysis</h4>Current EPA regulations focus on targeted analysis of specific PFAS compounds.Examples include:<ul><li>PFOA</li><li>PFOS</li></ul>Drinking water regulations currently establish maximum contaminant levels (MCLs) for these compounds at extremely low concentrations.Advantages of targeted LC-MS analysis include:<ul><li>Clearly defined analytes</li><li>Established regulatory limits</li><li>High analytical sensitivity</li><li>Reliable quantitation</li></ul>However, limitations remain:<ul><li>Only a small subset of PFAS is measured</li><li>Many PFAS compounds remain undetected</li><li>Total PFAS burden may be underestimated</li></ul>As Dr. Terry notes, targeted analysis provides only part of the overall picture.<h4>The Fluorine Mass Balance Problem</h4>One of the most important concepts discussed in the interview is fluorine mass balance.When PFAS degrade, fluorinated fragments may remain in the environment.This creates a critical question:Are treatment technologies truly destroying PFAS, or merely transforming them into shorter-chain PFAS compounds?To answer this, researchers perform fluorine mass balance calculations using:<ul><li>Targeted PFAS analysis</li><li>Fluoride measurements</li><li>Total organic fluorine measurements</li></ul>A successful destruction process should ultimately convert PFAS into fluoride ions rather than simply producing smaller PFAS molecules.<h4>Advanced Analysis, Treatment Technologies, and Future Directions</h4><h5>Combining Targeted and Bulk Fluorine Measurements</h5>Dr. Terry's group complements targeted LC-MS analysis with measurements of total organic fluorine.Approaches include:<ul><li>Absorbable Organic Fluorine (AOF)</li><li>Extractable Organic Fluorine (EOF)</li><li>Combustion Ion Chromatography (CIC)</li></ul>In these methods:<ul><li>Organic fluorine is captured</li><li>Samples are combusted</li><li>Released fluoride is quantified</li></ul>This provides insight into total fluorinated content, although contributions may originate from sources beyond PFAS, including pharmaceuticals and consumer products.<h4>Surprising Findings from Combined Analysis</h4>One important finding from wastewater studies was that targeted PFAS measurements accounted for only a small fraction of total organic fluorine.Dr. Terry reports that:<ul><li>Approximately 4% of measured AOF could be explained by targeted PFAS compounds</li><li>The majority of organic fluorine remained unidentified</li></ul>This suggests that environmental fluorine chemistry is considerably more complex than currently represented by routine monitoring programs.<h4>Preventing PFAS Contamination in the Laboratory</h4>PFAS analysis requires exceptionally strict quality control.Dr. Terry's laboratory routinely:<ul><li>Tests every new batch of SPE cartridges</li><li>Tests carbon tubes before use</li><li>Uses DI water blanks</li><li>Verifies consumables labeled as PFAS-free</li><li>Collects field blanks</li><li>Monitors analytical blanks throughout workflows</li></ul>These practices help prevent contamination from laboratory materials and consumables, a common challenge in ultra-trace PFAS analysis.<h4>Current PFAS Treatment Technologies</h4>Three technologies currently dominate PFAS removal in drinking water treatment:<ul><li>Granular Activated Carbon (GAC)</li><li>Ion Exchange Resins</li><li>Reverse Osmosis (RO)</li></ul>Selection depends on:<ul><li>PFAS concentration</li><li>Water quality</li><li>Local operating conditions</li><li>Utility infrastructure</li></ul>Although many emerging technologies are being investigated, these remain the most widely implemented solutions.<h4>Membrane Filtration and Pretreatment</h4>Dr. Terry collaborates with colleagues studying membrane-based PFAS treatment.Her own work focuses on pretreatment through biological filtration.The strategy involves:<ul><li>Removing natural organic matter</li><li>Reducing membrane fouling</li><li>Improving membrane performance</li><li>Extending operational efficiency</li></ul>This integrated approach demonstrates how multiple treatment technologies can work together to improve PFAS removal.<h4>Regional Monitoring Projects</h4>The University of Alabama team participates in EPA-supported monitoring efforts along the Gulf Coast.Their observations indicate:<ul><li>PFAS rarely occur alone</li><li>Multiple PFAS compounds frequently co-occur</li><li>Contamination often originates from identifiable point sources</li></ul>Examples include:<ul><li>Industrial facilities</li><li>Manufacturing operations</li><li>Military installations</li><li>Carpet manufacturing facilities</li></ul>Source identification remains a critical component of environmental monitoring programs.<h4>Future Directions for PFAS Monitoring</h4>Looking ahead, Dr. Terry sees several opportunities for improvement.Desired advances include:<ul><li>In situ monitoring technologies</li><li>Portable detection systems</li><li>Reduced analytical complexity</li><li>Lower instrument costs</li><li>Less expensive consumables</li><li>Faster analysis workflows</li></ul>Current PFAS testing requires substantial expertise, specialized instrumentation, and rigorous quality control, making widespread implementation challenging.<h4>Dr. Terry's Perspective on Long-Term Solutions</h4>When asked what major PFAS challenge she would most like to solve, Dr. Terry offered a broader perspective.Rather than focusing solely on remediation, she emphasized prevention.Her view is that society should:<ul><li>Better evaluate chemical toxicity before commercialization</li><li>Assess risks before large-scale deployment</li><li>Reduce introduction of harmful compounds into the environment</li><li>Support utilities tasked with contaminant removal</li></ul>She notes that water utilities often bear the responsibility of removing contaminants they did not create, highlighting the need for broader societal and regulatory support.<h4>Final Thoughts</h4>Dr. Terry encourages consumers to:<ul><li>Learn where their drinking water originates</li><li>Read annual water quality reports</li><li>Stay informed about local water quality</li><li>Consider appropriate home filtration where needed</li></ul>At the same time, she emphasizes confidence in municipal water systems and the importance of continued investment in water quality research, monitoring, and treatment technologies.<h4>Conclusion</h4>PFAS remain one of the most complex challenges in environmental chemistry and water treatment. Through advanced analytical methods such as LC-MS/MS, combustion ion chromatography, fluorine mass balance approaches, and large-scale monitoring programs, researchers like Dr. Leigh Terry are helping improve our understanding of these persistent contaminants.The interview highlights that solving the PFAS challenge will require a combination of better analytical tools, improved treatment technologies, stronger regulatory frameworks, and a greater emphasis on prevention before contaminants enter the environment.<blockquote>This text has been automatically transcribed from a video presentation using AI technology. It may contain inaccuracies and is not guaranteed to be 100% correct.</blockquote><blockquote><h4>Concentrating on Chromatography Podcast</h4>Dive into the frontiers of chromatography, mass spectrometry, and sample preparation with host David Oliva. Each episode features candid conversations with leading researchers, industry innovators, and passionate scientists who are shaping the future of analytical chemistry. From decoding PFAS detection challenges to exploring the latest in AI-assisted liquid chromatography, this show uncovers practical workflows, sustainability breakthroughs, and the real-world impact of separation science. Whether you’re a chromatographer, lab professional, or researcher you'll discover inspiring content!You can find Concentrating on Chromatography Podcast in podcast apps:<ul><li><a target="_blank" rel="noopener noreferrer" href="https://open.spotify.com/show/1wZg67rCjoGju4yhqxI3tL">Spotify</a></li><li><a target="_blank" rel="noopener noreferrer" href="https://podcasts.apple.com/tz/podcast/concentrating-on-chromatography/id1788429185">Apple podcast</a></li><li><a target="_blank" rel="noopener noreferrer" href="https://podscan.fm/podcasts/concentrating-on-chromatography">podscan</a></li></ul>and on <a target="_blank" rel="noopener noreferrer" href="https://www.youtube.com/@ChromatographyTalk/videos">YouTube channel</a></blockquote>

PFAS in Water: Why We’re Missing 96% of the Problem

This study evaluates an immobilized enzyme reactor (IMER) containing peptide N-glycosidase from Rudaea cellulosilytica (PNGase Rc) for rapid online deglycosylation of intact glycoproteins. Human glycoproteins and therapeutic antibodies were used as model substrates to assess reactor performance.The PNGase Rc IMER efficiently removed complex sialylated and core-fucosylated glycans, simplifying protein characterization and glycosylation-site analysis. The approach offers a fast and effective solution for bioanalytical workflows, biopharmaceutical quality control, and stability testing of therapeutic proteins.<blockquote><h4>The original article</h4><h4><a target="_blank" rel="noopener noreferrer" href="https://pubs.acs.org/doi/10.1021/acsomega.6c00577">Online Deglycosylation of Monomeric Intact Proteins Using the PNGase Rc Immobilized-Enzyme Reactor</a></h4>Katarína Molnárová, Petr Novák, Jana Nováková, and Petr Pompach*ACS Omega 2026, 11, 17, 25724–25729<a target="_blank" rel="noopener noreferrer" href="https://doi.org/10.1021/acsomega.6c00577">https://doi.org/10.1021/acsomega.6c00577</a><h6>licensed under <a target="_blank" rel="noopener noreferrer" href="https://creativecommons.org/licenses/by/4.0/">CC-BY 4.0</a></h6></blockquote>Selected sections from the article follow. Formats and hyperlinks were adapted from the original.<div>Protein glycosylation, in which monosaccharide units are attached to a protein backbone through enzymatic pathways, is involved in many important biochemical processes and is considered one of the most important post-translational modifications. <a href="javascript:void(0);">(1)</a> It affects not only the stability and secretion of proteins, cell–cell interactions, and receptor–ligand recognition mechanisms but also the biological activity, plasma half-life, immunogenicity, pharmacodynamics, and pharmacokinetics of biotherapeutics. <a href="javascript:void(0);">(2−5)</a> Therapeutical monoclonal antibodies (mAbs) are among the best known pharmaceuticals that are based on glycoproteins. <a href="javascript:void(0);">(6)</a> Over 90% of the mAbs are derived from immunoglobulin G (IgG), in which N-glycosylation occurs at Asn297 of the Fc region and is responsible for modulating immune responses and interactions with other proteins. <a href="javascript:void(0);">(7,8)</a> In cancer treatment, mAbs are used to increase the response of the immune system against the tumor cell, thus inhibiting the cancerous cell growth and helping with the destruction and elimination. <a href="javascript:void(0);">(9)</a> Among the most commonly used mAbs are trastuzumab, bevacizumab, cetuximab, or margetuximab, which are used as an additional treatment for breast, colorectal, prostate, or lung cancer. <a href="javascript:void(0);">(10)</a>Mass spectrometry (MS)-based methods have become a method of choice in the analysis of mAbs that can provide information on sequence, post-translational modifications, and glycosylation profiles. <a href="javascript:void(0);">(11)</a> The enzymatic removal of the attached glycans from the protein backbone can reduce the complexity of the MS analysis, which can lead to a better understanding of the protein structure and modifications. In glycoproteomics, peptide-N-glycosidase F (PNGase F) is most used for glycan removal. It cleaves the linkage between the Asn residue and the N-acetylglucosamine (GlcNAc). <a href="javascript:void(0);">(12)</a> Jmeian et al. developed a monolithic PNGase F enzyme reactor fabricated in fused silica using a glycidyl methacrylate-co-ethylene dimethacrylate polymer and integrated it into an LC–MS workflow. The authors demonstrated the applicability of this system for the characterization of released neutral and acidic N-glycans by LC–MS analysis. <a href="javascript:void(0);">(13)</a> In another study, oriented immobilization of PNGase F onto a methacrylate-based monolithic support via glutathione-S-transferase–glutathione affinity coupling was employed to release N-glycans from denatured ribonuclease B and fetuin, as well as from native IgG. The released glycans and the corresponding deglycosylated proteins were subsequently analyzed by MALDI-MS. <a href="javascript:void(0);">(14)</a>Gramlich et al. showed the potential of PNGase Rc for N-glycan hydrolysis from both tryptic peptides and native glycoproteins at a low pH. The favorable properties of PNGase Rc, including activity at acidic pH and compatibility with reducing agents such as tris(2-carboxyethyl)phosphine hydrochloride (TCEP) and denaturants like urea, make this enzyme well suited for online intact protein deglycosylation workflows. <a href="javascript:void(0);">(15,16)</a>In this report, we demonstrate the potential of the PNGase Rc IMER for rapid online deglycosylation of reduced monomeric glycoproteins, including human haptoglobin (Hp), human transferrin, anti-hHD6, and the therapeutic monoclonal antibodies trastuzumab and bevacizumab. The column retained its activity over more than 150 consecutive measurements, highlighting its robustness, suitability for repeated use in bioanalytical workflows, and applicability to pharmaceutical research and development.<h4>Experimental Section</h4><h5>Instrumentation and Experimental Conditions</h5>Automated sample handling and online deglycosylation were carried out using the HDX workstation (AffiPro, Czech Republic). The scheme of the system is shown on <a target="_blank" rel="noopener noreferrer" href="https://pubs.acs.org/doi/10.1021/acsomega.6c00577#fig1">Figure 1</a>. Samples of 50 mM ammonium bicarbonate buffer (pH 8.2) and 40 mM TCEP were placed in a sample tray at 4 °C. Ten microliters of the protein sample at a concentration of 0.03 mg/mL (0.3 μg per injection) were added to 40 μL of 50 mM ammonium bicarbonate and then transferred to 50 μL of 40 mM TCEP, pH 2.3. The disulfide bond reduction was carried at 40 °C for 5 min. The automated sample handling system injected 100 μL of the reaction mixture into the sample loop and switched the injection valve. Online deglycosylation was performed at room temperature using a PNGase Rc IMER (2.1 × 20 mm, AffiPro, Czech Republic) at a flow rate of 50 μL/min, followed by protein desalting on an OPTI-TRAP (0.5 × 2 mm) trap column (Optimize Technologies Inc., Oregon City, Oregon, USA) heated to 55 °C for 10 min with 0.2% formic acid delivered by an <a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/library?mainauthorItems=10&amp;search=1260+Infinity+III+isocratic+pump">Agilent 1260 Infinity III isocratic pump</a> (Agilent Technologies, Santa Clara, California, USA). By switching the trap valve, the protein was eluted by an acetonitrile gradient [(min)/% B] 0/10-9/10-16/70-16.5/90-17/90-18/10-23/10] delivered by the <a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/library?mainauthorItems=10&amp;search=1290+Infinity+II+LC">Agilent 1290 Infinity II HPLC</a> system (Agilent Technologies, Santa Clara, California, USA) at a flow rate of 20 μL/min. The mobile phase A consisted of 5% acetonitrile with 1% propan-2-ol and 0.1% formic acid, and the mobile phase B consisted of 95% acetonitrile with 1% propan-2-ol and 0.1% formic acid. The <a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/library?mainauthorItems=8&amp;search=maXis+Q-TOF">maXis Q-TOF mass spectrometer</a> (Bruker Daltonics, Bremen, Germany) was operated in the survey MS mode in a mass range of m/z 50–3000. The acquired data were analyzed and processed by DataAnalysis 4.4 (Bruker Daltonics) and deconvoluted by UniDec <a href="javascript:void(0);">(17)</a> with the following parameters: data processing range, m/z 500–3000; charge range, 1–80; allowed mass range, 1,000–80,000; sampling rate, 10 Da; peak detection range, 500 Da; and peak detection threshold, 0.1.<img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/ACS_Omega_2026_11_17_25724_25729_Figure_1_Schematic_of_the_automated_sample_handling_system_for_online_protein_deglycosylation_and_desalting_d4ee48b627.jpg" alt="ACS Omega 2026, 11, 17, 25724–25729: Figure 1. Schematic of the automated sample handling system for online protein deglycosylation and desalting. Protein samples, along with 50 mM ammonium bicarbonate and 40 mM TCEP stock solutions were placed in a sample tray chilled to 4 °C, while a second tray heated to 40 °C was used for disulfide bond reduction with TCEP buffer." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_ACS_Omega_2026_11_17_25724_25729_Figure_1_Schematic_of_the_automated_sample_handling_system_for_online_protein_deglycosylation_and_desalting_d4ee48b627.jpg 245w," sizes="100vw" width="1287" height="386"><h4>Results and Discussion</h4>The performance of the PNGase Rc IMER for the removal of complex N-glycans from intact proteins was tested using heavily glycosylated human Hp with four glycosylation sites (Asn184, Asn207, Asn211, and Asn241) <a href="javascript:void(0);">(18)</a> located at the β-chain and occupied by complex N-glycans terminated with sialic acid. Haptoglobin phenotype 2-1, used in this study, forms multimers consisting of β-chain and disulfide linked α1 and α2 chains. <a href="javascript:void(0);">(19)</a> The detection of glycosylated Hp β-chain requires reduction of the disulfide bonds by incubating the protein with a reducing reagent. TCEP is a common reducing reagent used for fast reduction of disulfide bonds at various pH levels. Forty degrees Celsius, the maximum temperature of the PAL tray, and 5 min of incubation were sufficient for complete reduction of Hp disulfide bonds. Individual signals corresponding to the glycosylated β-chain were observed in the m/z range of 35,800–37,400 (<a target="_blank" rel="noopener noreferrer" href="https://pubs.acs.org/doi/10.1021/acsomega.6c00577#fig2">Figure 2</a>a). The limited resolution of the mass spectrometer did not allow assigning the different glycoforms of the haptoglobin β-chain, which is occupied by biantennary, triantennary, or tetraantennary glycans with various amounts of sialic acid and fucose. <a href="javascript:void(0);">(20,21)</a> The nonglycosylated α1 and α2 chains were observed at m/z 9190 and 15,940. When the PNGase Rc IMER was integrated into the flow system, a signal at m/z 27,270 was detected, demonstrating complete deglycosylation of the haptoglobin β-chain (<a target="_blank" rel="noopener noreferrer" href="https://pubs.acs.org/doi/10.1021/acsomega.6c00577#fig2">Figure 2</a>b).<img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/ACS_Omega_2026_11_17_25724_25729_Figure_2_A_Mass_spectrum_of_human_Hp_without_the_PN_Gase_Rc_IMER_implemented_in_the_system_0ecca84ed4.jpg" alt="ACS Omega 2026, 11, 17, 25724–25729: Figure 2. (A) Mass spectrum of human Hp without the PNGase Rc IMER implemented in the system. The base peak signals of the spectrum correspond to both α1 and α2 chains. The signal of the β chain is spread across its different glycoforms. (B) Mass spectrum of human Hp with the PNGase Rc IMER implemented in the system. The signal of the deglycosylated β chain is higher compared to its glycosylated form and observed only at m/z 27,270. This indicates complete removal of glycans from the Hp β chain." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_ACS_Omega_2026_11_17_25724_25729_Figure_2_A_Mass_spectrum_of_human_Hp_without_the_PN_Gase_Rc_IMER_implemented_in_the_system_0ecca84ed4.jpg 245w," sizes="100vw" width="2015" height="913">In a previous study by Krenkova et al., the PNGase F IMER was used for deglycosylation of IgG, RNase B, and bovine fetuin with follow-up mass spectrometry detection of both released glycans and intact proteins using MALDI-TOF MS. <a href="javascript:void(0);">(14)</a> The authors demonstrated an effective method for rapid protein deglycosylation, which, however, requires additional sample handling prior to mass spectrometry measurement. The other study by Jmeian et al., which focused on released N-glycans, implemented a PNGase F IMER with online LC–MS detection. <a href="javascript:void(0);">(13)</a> The authors show the effectiveness of the method by identifying many different glycans released from a single protein or from a complex plasma mixture. Our approach is not directly intended for released N-glycan analysis; instead, it demonstrates the ability to measure intact proteins after rapid online deglycosylation using PNGase Rc at low pH. Samples prepared immediately prior to mass spectrometry analysis using the automated sample-handling platform can reduce variability associated with autosampler residence time, as some proteins may undergo degradation or precipitation during storage in the autosampler.Although all glycoproteins in this study were effectively deglycosylated, the method’s efficiency depends on the characteristics of the individual glycoprotein. Therefore, optimizing method parameters─including the isocratic flow rate, glycoprotein incubation time with TCEP, and/or the addition of denaturing reagent─may improve the overall efficiency of the deglycosylation process.The PNGase Rc IMER stability over a series of injections was tested by using the trastuzumab antibody. The measurements were carried out with the enzyme column kept at the laboratory temperature. As shown in <a target="_blank" rel="noopener noreferrer" href="https://pubs.acs.org/doi/10.1021/acsomega.6c00577#fig5">Figure 5</a>, the results indicate that the column performance remained stable over the course of the 150 measurements, and no glycosylated form of the trastuzumab antibody heavy chain was detected. The activity of PNGase Rc in the presence of 5% acetonitrile and 5% propan-2-ol was evaluated using a bottom-up approach with human 2–1 haptoglobin, which was online-digested by a coimmobilized nepenthesin-2/pepsin IMER. In the absence of an organic solvent, the peptide IEKVVLHPNYSQVD remained fully deglycosylated throughout 30 injections. In contrast, in the presence of organic solvent, a marked loss of enzymatic activity was observed after only a few injections (<a target="_blank" rel="noopener noreferrer" href="https://pubs.acs.org/doi/suppl/10.1021/acsomega.6c00577/suppl_file/ao6c00577_si_001.pdf">Figure 2</a>).<img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/ACS_Omega_2026_11_17_25724_25729_Figure_5_Monitoring_the_stability_and_performance_of_PN_Gase_Rc_IMER_4a9b5a9c53.jpg" alt="ACS Omega 2026, 11, 17, 25724–25729: Figure 5. Monitoring the stability and performance of PNGase Rc IMER. Trastuzumab heavy chain was completely deglycosylated after 150 injections." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_ACS_Omega_2026_11_17_25724_25729_Figure_5_Monitoring_the_stability_and_performance_of_PN_Gase_Rc_IMER_4a9b5a9c53.jpg 170w," sizes="100vw" width="996" height="918"><h4>Conclusions</h4>Here, we demonstrate the potential of the PNGase Rc IMER for the online removal of N-glycans from intact monomeric glycoproteins reduced with TCEP. The column showed high sensitivity toward Hp, which has multiple glycosylation sites with various glycan structures, confirming its suitability for the profiling of complex glycoproteins. Bevacizumab and trastuzumab exhibited similar glycosylation profiles, with core-fucosylated biantennary glycans being the most abundant. Online deglycosylation with the PNGase Rc column resulted in a complete removal of the glycans from the heavy chains of both antibodies, proving that the column can be used for the rapid online deglycosylation of intact therapeutic mAbs. Moreover, the column remained stable over 150 measurements without any loss of activity, even when it was maintained at laboratory temperature. The significantly reduced reaction time, together with full automation and the absence of manual handling steps, substantially improves analytical throughput compared to conventional off-line deglycosylation workflows. These findings highlight the potential of the PNGase Rc IMER in the development of glycoprotein-based biopharmaceuticals, where detailed structural characterization of the protein part is critical for quality control, monitoring batch-to-batch variability, and stability testing.</div>

Online Deglycosylation of Monomeric Intact Proteins Using the PNGase Rc Immobilized-Enzyme Reactor

In the week of June 29, the following webinars await you in the field of liquid phase and especially HPLC and LC/MS.<blockquote><h4><a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/webinars">👉 DETAILS AND REGISTRATION IN THE WEBINARS SECTION</a></h4>👉 Search and filter in the largest global database with over 5 500 GC, LC, MS and spectroscopy webinars.</blockquote><h5>1. Thermo Fisher Scientific: Insights from an IC specialist: To be suppressed or not suppressed?</h5><ul><li>Tu, 30.6.2026 09:00 CEST</li><li><a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/webinar/6310">Registration</a></li></ul>Join Thermo Fisher’s Ask the Experts session to learn how suppressor technology impacts ion chromatography performance, sensitivity, reliability, maintenance, and troubleshooting.<h5>2. SCIEX: Advancing Proteomics with ZenoTOF: From Single-Cell Analysis to Plasma Biomarker Discovery</h5><ul><li>Tu, 30.6.2026 11:30 CEST</li><li><a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/webinar/6311">Registration</a></li></ul>Discover how ZenoTOF high-resolution mass spectrometry is accelerating single-cell proteomics and plasma biomarker discovery through enhanced sensitivity, throughput, and automation.<h5>3. Agilent Technologies: Measuring Diverse Samples with UV/VIS Spectrometers and Integrating Spheres</h5><ul><li>Tu, 30.6.2026 20:30 CEST</li><li><a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/webinar/6052">Registration</a></li></ul>This webinar will discuss diffuse transmission and reflection UV/VIS measurements, including how to perform measurements with Agilent’s Cary 60 UV/VIS Spectrometer.<h5>4. Waters Corporation: Informatics series #33 Tips, Tricks and Best Practices</h5><ul><li>Th, 2.7.2026 11:00 CEST</li><li><a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/webinar/6315">Registration</a></li></ul>This interactive session revisits essential features, shortcuts, and practical techniques in Empower software that can streamline everyday laboratory work and reduce unnecessary effort.<h5>5. Waters Corporation: Unlocking Therapeutic Peptide Characterisation (GLP-1RAs) with Cyclic IMS</h5><ul><li>Th, 2.7.2026 15:00 CEST</li><li><a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/webinar/6313">Registration</a></li></ul>Discover how cyclic ion mobility spectrometry (cyclic IMS) improves characterization of GLP-1 analogues and therapeutic peptides, supporting development, quality control, and regulatory submissions.<h5>6. Agilent Technologies: Spectral Flow Cytometry Masterclass - Session 1</h5><ul><li>Fr, 3.7.2026 07:00 CEST</li><li><a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/webinar/6316">Registration</a></li></ul>Learn how full-spectrum flow cytometry enables high-dimensional cell analysis. Explore spectral flow technology, experimental design, and applications in immunology, oncology, and vaccine research.

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Organomation, founded in 1959, designs and manufactures high-quality nitrogen evaporators and extraction systems. Known for innovation and durability, their lab instruments are used globally for efficient sample preparation with strong customer support.

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Thermo Scientific™ GENESYS™ 30 Vis Spectrophotometer

Perform both basic and complex analyses with the Thermo Scientific™ GENESYS™ 30 Visible Spectrophotometer, an intuitive, reliable solution for education or in the lab.

<h3>Designed for Users, Designed for Results</h3>The Thermo Scientific ™ GENESYS ™ 30 is the most thoughtfully designed spectrophotometer available today. Its high resolution color display, large sample compartment, and data export options meet modern expectations for students, educators and lab technicians. The GENESYS 30 blends the simplicity to satisfy novice students with the capabilities that labs need for accurate quantitative analysis.<h4>System Benefits:</h4><ul><li>Large sample compartment with front access simplifies sample handling</li><li>High resolution color display</li><li>Outstanding durability in challenging environments thanks to:</li><li>Waterproof screen</li><li>Sealed, rubberized keypad</li><li>Removable, washable sample compartment</li><li>Sloping surfaces that shed spills</li><li>Chemical resistant PC-ABS covers</li><li>3A linear measurement range for concentrated solutions</li><li>Multi-point quant offers higher measurement confidence</li><li>Traditional monochromator paired with advanced electronics delivers outstanding stability</li><li>Scan mode for spectral analysis and interpretation</li><li>Data any way you want it - excellent snap-on thermal printer or USB data export</li><li>Expanded capabilities with optional VISIONlite software</li><li>2-year warranty</li></ul><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Thermo_Scientific_GENESYS_30_Vis_Spectrophotometer_4_4442fcccc5.jpg" alt="Thermo Scientific™ GENESYS™ 30 Vis Spectrophotometer." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Thermo_Scientific_GENESYS_30_Vis_Spectrophotometer_4_4442fcccc5.jpg 195w," sizes="100vw" width="2103" height="1685"><h3>Modern Power meets Traditional Simplicity</h3><h4>Live Display and Concentration Mode</h4>Replicate the familiar display of absorbance or transmittance, and single standard concentration measurements of prior generation instruments in the GENESYS 30 Live Display mode. All your parameters are set and kept in view on a single screen as the calculated concentration updates every two seconds.<h4>Fixed Mode</h4>Apply a factor to an absorbance measurement at one wavelength, or measure at two wavelengths with two factors and perform addition, subtraction or division of the values. Display results for up ten samples then print, save or clear your data before going on to the next sample series.<h4>Quantitative Analysis with up to Six Standards</h4>Go beyond the basic spectrophotometer by recording and storing up to six standards to calibrate your method. Choose a linear fit with an intercept or forced through zero. Name and save your method on the instrument or back it up to a USB memory device. All data are recorded with an index number (S1 – S10) and a time stamp. The result table accommodates up to ten samples for you to print or save before going on to the next sample series or analysis.<h4>Go negative!</h4>Some methods call for blanking the instrument on a dark standard and reading absorbance in the negative range. Most single beam instruments cannot measure below -0.3A. GENESYS 30 uses advanced signal processing to deliver stable, reliable results down to -3A.<h4>Simple to Sophisticated:</h4>The GENESYS 30 has the flexibility to perform routine analysis one minute and complex analytical methods the next. The custom Analyzer mode enables you to build your formula with up to six wavelengths, six factors, standard arithmetic operators and absorbance values raised to the 2nd or 3rd power. Use the included Windows® application to design your method. Transfer the method to your GENESYS 30 and run it with the touch of a button. Analyzer mode shows your method name and a continuous display of your results. Technicians can write the result down or print it with a time stamp using the printer accessory.<h4>Smarter by Design:&nbsp;</h4>GENESYS 30 offers the convenient SmartStart™ feature that has delighted GENESYS 10S users since its introduction. The SmartStart menu gives you direct access to your 12 favorite methods. Allowing you to work smarter, not harder.<h4>Snap on Printer:&nbsp;</h4>Fast and clear printing when you need it. Add printing capability to your instrument. The snap-on thermal printer installs in moments.&nbsp;<h4>Quick and Simple Lamp Replacement:&nbsp;</h4>No need to remove the cover, just loosen two screws, slide out the old lamp fitting and slide a new one back in. Everything is pre-aligned, just let the new lamp warm up and go back to work.<img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Thermo_Scientific_GENESYS_30_Vis_Spectrophotometer_3_82082740d3.jpg" alt="Thermo Scientific™ GENESYS™ 30 Vis Spectrophotometer: Quick and simple lamp replacement." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Thermo_Scientific_GENESYS_30_Vis_Spectrophotometer_3_82082740d3.jpg 197w," sizes="100vw" width="1737" height="1376"><h3>At Home in the Instructional Laboratory</h3>GENESYS spectrophotometers are core teaching instruments in chemistry and biology laboratories around the world. The Thermo Scientific™ GENESYS™ 30 allows students and teachers to focus on examining scientific data and learning scientific concepts instead of learning instrumentation.<h4>Easy to Teach, Easy to Learn&nbsp;</h4>Clearly labeled, uniquely colored keys simplify instrument operation. New users require little or no training.<h4>Get Working – Faster!</h4>Large, color digital screen and numeric keypad help you set up methods faster. Simplified software interface gives users maximum control and efficiency.<h4>Designed for Today, Built for Tomorrow – Inside and Out!</h4>On the inside, GENESYS 30 uses advanced optics, electronics and signal processing to deliver superior measurement performance for its class. On the outside, GENESYS 30 presents a tough exterior built to last for years in the most demanding environments.<ul><li>Resistant to spills</li><li>Removable and washable sample compartment</li><li>The sample compartment lid<ul><li>stays where you put it - open or closed</li><li>has a magnetic latch to hold it closed during measurements</li><li>is customer-replaceable should it get broken through abuse</li></ul></li></ul><h4>Simple and Familiar</h4>Open the Live Display application with a single button-press to monitor the absorbance or transmittance live – just like a GENESYS 20 or SPECTRONIC 20. The spectrum indicator shows the color of light being absorbed.<h4>Special support for OD 600 and test-tube measurements</h4>For measurements in test-tubes, the test-tube holder accessory accommodates 100mm x 13mm test-tubes with the lid closed. Or add the tall test-tube adaptor to use larger diameter, taller tubes (up to 150mm, 6 inches) with the lid open. Special OD600 mode for cell growth measurements. Select it from the Home menu and go! No method setup required.<h4>Scanning: Simple and Fast</h4>Find peaks in the spectrum of your sample by scanning across the spectrum in any range from 325nm to 1100nm with 1nm precision and noise-free peaks up to 3A. Use the cursor and zoom controls to identify λmax.<h4>Data you can use</h4><ul><li>Print your scan and stick it into a lab notebook using the printer accessory and optional self-stick printer paper.</li><li>Save the data points of your scan to a USB memory device and load the data into a spreadsheet or graphing program for optimal reporting.</li></ul><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Pragolab_Thermo_Scientific_Genesys_30_USB_stick_A_c45fd86787.jpg" alt="Thermo Scientific™ GENESYS™ 30 Vis Spectrophotometer: USB stick." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Pragolab_Thermo_Scientific_Genesys_30_USB_stick_A_c45fd86787.jpg 181w," sizes="100vw" width="543" height="469"><h3>VISIONlite Software</h3>For users who need the power of computerized data analysis, optional Thermo Scientific™ VISIONlite™ software pairs a simple user interface with a wider range of options for experiment design and data analysis.<img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Thermo_Scientific_Genesys_spectrophotometers_ae282fe726.jpg" alt="Thermo Scientific - Genesys spectrophotometers: VISIONlite Software." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Thermo_Scientific_Genesys_spectrophotometers_ae282fe726.jpg 239w," sizes="100vw" width="1422" height="928"><h4>Scan Application:</h4><ul><li>Automated peak pick and label</li><li>Overlay spectra and print the results</li></ul><h4>Kinetics Application:</h4><ul><li>Data intervals as low as 1 second</li><li>Enzyme rate calculation with factor</li></ul><h4>Quantitative Analysis:</h4><ul><li>Up to 100 standards</li><li>Linear, quadratic or cubic data fits</li></ul><h4>Performance Verification:</h4><ul><li>Full suite of tests to verify performance of your instrument</li><li>Pre-loaded pass/fail limits specific to your instrument</li></ul><h3>GENESYS 30 Accessories</h3><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Thermo_Scientific_GENESYS_30_Vis_Spectrophotometer_59d912ddd3.jpg" alt="Thermo Scientific™ GENESYS™ 30 Vis Spectrophotometer: GENESYS 30 Accessories." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Thermo_Scientific_GENESYS_30_Vis_Spectrophotometer_59d912ddd3.jpg 243w," sizes="100vw" width="1444" height="929"><h3>GENESYS 30 Vis Spectrophotometer Specifications</h3><ul><li>Baseline Flatness: &lt;0.003 A, 325 to 1000 nm smoothed</li><li>Compartments:&nbsp;<ul><li>Accessible from top, front or side</li><li>Able to accommodate cells up to 100mm pathlength</li><li>Available accessory for test-tubes up to 25mm diameter and 150mm height</li><li>Removable, washable sample compartment liner with magnetic placement and hold-down</li></ul></li><li>Connections: USB-A, USB-B</li><li>Description: GENESYS 30 VIS Spectrophotometer with EU Power Cable</li><li>Dimensions (L x W x H): 35.5 x 38.5 x 19.5 cm</li><li>Drift: &lt;0.002 A/hr. (At 500 nm after 2 hr. warmup.) Typical &lt;0.004 A/hr. after 1 hr. warmup</li><li>Electrical Requirements: External AC to DC converter. Voltage and Frequency (Hz) selected automatically, 100/250 V, 50/60 Hz</li><li>Keypad: Tactile rubber 23 keys with numeric keypad</li><li>Lamp Life: 1000 hrs.</li><li>Noise: (RMS at 500nm. 60 consecutive measurements)<ul><li>≤0.001A at 0A</li><li>≤0.001A at 1A</li><li>≤0.002A at 2A</li></ul></li><li>Optical Design: Single Beam</li><li>Photometric Accuracy: ±0.002 A (0 to 0.3 A), 0.5% of ABS reading (0.301 A to 2.5 A)</li><li>Photometric Display: -3A to +3A, 0 to 200 000 %T, 0 to 9 999 999 C</li><li>Photometric Range: -3A to +3A</li><li>Photometric Repeatability: ± 0.002 A (Measured at 1.0 A at 546 nm)</li><li>Printer Included: Thermal printer accessory with 80 mm width paper</li><li>Scan Speed: Automatic – up to 1200 nm/min.</li><li>Stray Light: &lt;0.1%T at 340 nm and 400 nm</li><li>Measured using glass cutoff filters or NaNO₂ at 340 nm</li><li>Warranty: 2 years standard with 1 year extension upon registration of the instrument with Thermo Fisher Scientific within 6 months of purchase</li><li>Wavelength Accuracy: ±2 nm</li><li>Wavelength Data Interval: 1 nm</li><li>Wavelength Repeatability: &lt; ±1 nm</li><li>Weight (Metric): 7.5 kg</li><li>Detector Type: Silicon Photodiode</li><li>Display: 5 in. 32-bit Color Display, 800 x 480 pixels</li><li>Languages: English, French, German, Spanish, Italian, Portuguese, Russian, Chinese, Japanese, Korean, Thai, Arabic</li><li>Wavelength Range: 325 to 1100 nm</li><li>Unit Size: Each</li></ul>

Thermo Scientific™ GENESYS™ 40 Vis Spectrophotometer

Perform routine measurements for quality control or teaching in the chemistry classroom with rugged Thermo Scientific™ GENESYS 40 Vis Spectrophotometer.

<h4>GENESYS - developed for users, designed for results</h4>Built on the foundation of 60 years of spectroscopy experience, the next generation Thermo Scientific™ GENESYS™ family of Vis/UV-Vis spectrophotometers provides exceptional performance with a contemporary design.<ul><li>Start taking measurements immediately with the simple-to-use, high-resolution, color-display start menu</li><li>Exchange samples with ease with large, accessible sample compartment</li><li>Save your data fast any way you want it with multiple data-export options</li></ul>There is a GENESYS spectrophotometer you can count on to provide accurate, reproducible results for your application requirements.<h3>GENESYS 40 Vis Spectrophotometer</h3>For teaching labs, research labs and industrial QC requiring robust construction, ease-of-use and software capability.<h5>Advanced usability, modern functionality</h5><ul><li>7-inch, high-resolution touchscreen interface</li><li>Local control increases speed and reliability for routine analysis</li><li>Large sample compartment with front access simplifies sample handling</li><li>Wi-Fi ready - save data and methods, print results from on-board control</li></ul><h5>Designed for</h5><ul><li>Chemistry labs</li><li>Routine measurements</li><li>Moderate throughput requirements</li></ul><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Pragolab_Thermo_Scientific_Genesys_40_Kyveta_A_7955a6a01a.jpg" alt="Thermo Scientific™ GENESYS™ 40 Vis Spectrophotometer" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Pragolab_Thermo_Scientific_Genesys_40_Kyveta_A_7955a6a01a.jpg 234w," sizes="100vw" width="1536" height="1024"><h3>Walk-up convenience</h3>Simple-to-use, touchscreen user interface features the methods and applications most important to you.<img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Thermo_Scientific_Genesys_spectrophotometers_ae282fe726.jpg" alt="Thermo Scientific GENESYS Spectrophotometers" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Thermo_Scientific_Genesys_spectrophotometers_ae282fe726.jpg 239w," sizes="100vw" width="1422" height="928"><h5>Flexible computer control</h5>Optional VISIONlite software offers complete control from a Microsoft ® Windows ® computer providing applications for:<ul><li>Scanning</li><li>Quantitative analysis</li><li>Kinetics</li><li>Fixed wavelength measurements</li></ul><h3>GENESYS 40 Vis Spectrophotometer Specifications</h3><ul><li>Baseline Flatness: ±0.005 A</li><li>Compartments:&nbsp;<ul><li>Accessible from top, front or side</li><li>Able to accommodate cells up to 100mm cells</li><li>Available accessory for test tubes up to 25 mm diameter and 150 mm height</li><li>Removable, washable sample compartment liner with magnetic placement and hold-down</li></ul></li><li>Connections:&nbsp;<ul><li>Single USB-A</li><li>Duplex USB-A</li><li>Export data to network or PC via USB, Ethernet, or Wi-Fi USB adaptor, available&nbsp;</li><li>Print via USB, Ethernet or Wi-Fi USB adaptor available</li></ul></li><li>Dimensions (L x W x H): 35.5 x 38.5 x 19.5 cm</li><li>Drift: (At 500 nm after 1 hour warmup) &lt;0.0010 A/hr.</li><li>Electrical Requirements: External AC to DC converter. Voltage and Frequency (Hz) selected automatically, 100/240 V, 50/60 Hz</li><li>Includes: GENESYS 40 Spectrophotometer</li><li>Keypad: Touchscreen</li><li>Lamp Life: &gt;1000 hrs.</li><li>Noise: (RMS at 500 nm 60 consecutive measurements)<ul><li>≤0.00020A at 0A at 260 and 500 nm</li><li>≤0.00030A at 1A at 260 and 500 nm</li><li>≤0.00040A at 2A at 260 and 500 nm</li></ul></li><li>Optical Design: Dual Beam</li><li>Photometric Accuracy: ±0.002 A at 0.5 A, ±0.004 A at 1.0 A, ±0.008 A at 2.0 A</li><li>Photometric Display: -3A to +5A</li><li>Photometric Range: –3A to +3.5A</li><li>Photometric Repeatability: ±0.001A at 1A measured at 1.0A at 546 nm</li><li>Printer Included: Snap-on printer available</li><li>Scan Speed: Automatic – up to 1800 nm/min.</li><li>Stray Light: &lt;0.05%T at 340 nm and 400 nm</li><li>Warranty: 2 yrs std + 1 yr ext upon reg</li><li>Wavelength Accuracy: ±0.5 nm</li><li>Wavelength Data Interval: 0.2 nm, 0.5 nm, 1 nm, 2 nm, 5 nm</li><li>Wavelength Repeatability: &lt; ±0.2 nm</li><li>Weight (Metric): 7.5 kg</li><li>Detector Type: Dual Silicon Photodiodes</li><li>Display: 7 in. High Definition Color Touchscreen, Fixed, 800 x 1280 pixels</li><li>Light Source: Tungsten Halogen</li><li>Spectral Bandwidth: 5 nm</li><li>Wavelength Range: 325 to 1100 nm</li><li>Unit Size: Each</li></ul>

Thermo Scientific™ GENESYS™ 140 Vis Spectrophotometer

Perform quantitative measurements in routine industrial QA/QC or university chemistry labs with the automated and network-capable Thermo Scientific™ GENESYS 140 Vis Spectrophotometer.

<h4>GENESYS - developed for users, designed for results</h4>Built on the foundation of 60 years of spectroscopy experience, the next generation Thermo Scientific™ GENESYS™ family of Vis/UV-Vis spectrophotometers provides exceptional performance with a contemporary design.<ul><li>Start taking measurements immediately with the simple-to-use, high-resolution, color-display start menu</li><li>Exchange samples with ease with large, accessible sample compartment</li><li>Save your data fast any way you want it with multiple data-export options</li></ul>There is a GENESYS spectrophotometer you can count on to provide accurate, reproducible results for your application requirements.<h3>GENESYS 140 Vis Spectrophotometer</h3>For advanced teaching labs, R&amp;D, research and life science applications requiring higher throughput or temperature control.<h5>Elite performance, enhanced capabilities</h5><ul><li>7-inch, high-resolution touchscreen interface</li><li>Local control increases speed and reliability for routine analysis</li><li>Large sample compartment with front access simplifies sample handling</li><li>Wi-Fi ready - save data and methods, print results from on-board control</li></ul><h5>Designed for</h5><ul><li>Chemistry labs</li><li>Routine measurements</li><li>Moderate throughput requirements</li></ul><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Pragolab_Thermo_Scientific_vis_spektrofotometr_genesys_140_titul_B_bd2a19cbcb.jpg" alt="Thermo Scientific GENESYS Spectrophotometers" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Pragolab_Thermo_Scientific_vis_spektrofotometr_genesys_140_titul_B_bd2a19cbcb.jpg 225w," sizes="100vw" width="516" height="358"><h3>Walk-up convenience</h3>Simple-to-use, touchscreen user interface features the methods and applications most important to you.<img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Thermo_Scientific_Genesys_spectrophotometers_ae282fe726.jpg" alt="Thermo Scientific: Genesys spectrophotometers" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Thermo_Scientific_Genesys_spectrophotometers_ae282fe726.jpg 239w," sizes="100vw" width="1422" height="928"><h5>Flexible computer control</h5>Optional VISIONlite software offers complete control from a Microsoft ® Windows ® computer providing applications for:<ul><li>Scanning</li><li>Quantitative analysis</li><li>Kinetics</li><li>Fixed wavelength measurements</li></ul><h3>GENESYS 140 Vis Spectrophotometer Specifications</h3><ul><li>Baseline Flatness: ±0.005 A</li><li>Compartments: Accommodates (optional):<ul><li>8-position cell changer</li><li>4-position cell changer (long path cells)</li><li>Peltier thermostatted cell holder (20–60 °C)</li><li>Sipper accessory</li><li>Fiber optic probe coupler</li></ul></li><li>Connections:&nbsp;<ul><li>Single USB-A</li><li>Duplex USB-A</li><li>Export data to network or PC via USB, Ethernet, or Wi-Fi USB adaptor, available&nbsp;</li><li>Print via USB, Ethernet or Wi-Fi USB adaptor available</li></ul></li><li>Dimensions (L x W x H): 35.5 x 38.5 x 19.5 cm</li><li>Drift: (At 500 nm after 1 hour warmup) &lt;0.0010 A/hr.</li><li>Electrical Requirements: External AC to DC converter. Voltage and Frequency (Hz) selected automatically, 100/240 V, 50/60 Hz</li><li>Includes: Genesys 140 Spectrophotometer</li><li>Keypad: Touchscreen</li><li>Lamp Life: &gt;1000 hrs.</li><li>Noise: (RMS at 500 nm 60 consecutive measurements)<ul><li>≤0.00020A at 0A at 260 and 500 nm</li><li>≤0.00030A at 1A at 260 and 500 nm</li><li>≤0.00040A at 2A at 260 and 500 nm</li></ul></li><li>Optical Design: Dual Beam</li><li>Photometric Accuracy: ±0.002A at 0.5A, ±0.004A at 1.0 A±0.008 A at 2.0A</li><li>Photometric Display: -3A to +5A</li><li>Photometric Range: –3A to +3.5A</li><li>Photometric Repeatability: ±0.001 A at 1 A measured at 1.0 A at 546 nm</li><li>Printer Included: Snap-on printer available</li><li>Scan Speed: Automatic – up to 1800 nm/min.</li><li>Stray Light: &lt;0.05%T at 340 nm and 400 nm</li><li>Warranty: 2 yrs std + 1 yr ext upon reg</li><li>Wavelength Accuracy: ±0.5 nm</li><li>Wavelength Data Interval: 0.2 nm, 0.5 nm, 1 nm, 2 nm, 5 nm</li><li>Wavelength Repeatability: &lt; ±0.2 nm</li><li>Weight (Metric): 7.5 kg</li><li>Detector Type: Dual Silicon Photodiodes</li><li>Display: 7 in. High Definition Color Touchscreen, Tiltable, 800 x 1280 pixels</li><li>Light Source: Tungsten Halogen</li><li>Spectral Bandwidth: 5 nm</li><li>Wavelength Range: 325 to 1100 nm</li><li>Unit Size: Each</li></ul>

Thermo Scientific™ GENESYS™ 50 UV-Vis Spectrophotometer

Perform routine measurements for quality control or teaching in the chemistry classroom with rugged Thermo Scientific™ GENESYS 50 UV-Vis Spectrophotometer.

<h4>GENESYS - developed for users, designed for results</h4>Built on the foundation of 60 years of spectroscopy experience, the next generation Thermo Scientific™ GENESYS™ family of Vis/UV-Vis spectrophotometers provides exceptional performance with a contemporary design.<ul><li>Start taking measurements immediately with the simple-to-use, high-resolution, color-display start menu</li><li>Exchange samples with ease with large, accessible sample compartment</li><li>Save your data fast any way you want it with multiple data-export options</li></ul>There is a GENESYS spectrophotometer you can count on to provide accurate, reproducible results for your application requirements.<h3>GENESYS 50 UV-Vis Spectrophotometer</h3>For teaching labs, research labs and industrial QC requiring robust construction, ease-of-use and software capability.<h5>Advanced usability, modern functionality</h5><ul><li>7-inch, high-resolution touchscreen interface</li><li>Local control increases speed and reliability for routine analysis</li><li>Large sample compartment with front access simplifies sample handling</li><li>Wi-Fi ready - save data and methods, print results from on-board control</li></ul><h5>Designed for</h5><ul><li>Chemistry labs</li><li>Routine measurements</li><li>Moderate throughput requirements</li></ul><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Pragolab_Thermo_Scientific_Genesys_50_Kyveta_bae9677ae0.jpg" alt="Thermo Scientific™ GENESYS™ 50 UV-Vis Spectrophotometer" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Pragolab_Thermo_Scientific_Genesys_50_Kyveta_bae9677ae0.jpg 188w," sizes="100vw" width="534" height="443"><h3>Walk-up convenience</h3>Simple-to-use, touchscreen user interface features the methods and applications most important to you.<img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Thermo_Scientific_Genesys_spectrophotometers_ae282fe726.jpg" alt="Thermo Scientific GENESYS Spectrophotometers" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Thermo_Scientific_Genesys_spectrophotometers_ae282fe726.jpg 239w," sizes="100vw" width="1422" height="928"><h5>Flexible computer control</h5>Optional VISIONlite software offers complete control from a Microsoft ® Windows ® computer providing applications for:<ul><li>Scanning</li><li>Quantitative analysis</li><li>Kinetics</li><li>Fixed wavelength measurements</li></ul><h3>GENESYS 50 UV-Vis Spectrophotometer Specifications</h3><ul><li>Baseline Flatness: ±0.002 A</li><li>Compartments:&nbsp;<ul><li>Accessible from top, front or side</li><li>Able to accommodate cells up to 100 mm cells</li><li>Available accessory for test-tubes up to 25 mm diameter and 150 mm height</li><li>Removable, washable sample compartment liner with magnetic placement and hold-down</li></ul></li><li>Connections:&nbsp;<ul><li>Single USB-A</li><li>Duplex USB-A</li><li>Export data to network or PC via USB, Ethernet, or Wi-Fi USB adaptor, available</li><li>Print via USB, Ethernet or Wi-Fi USB adaptor available</li></ul></li><li>Dimensions (L x W x H): 35.5 x 38.5 x 19.5 cm</li><li>Drift: (At 500 nm after 1 hour warmup) &lt;0.0005 A/hr.</li><li>Electrical Requirements: External AC to DC converter. Voltage and Frequency (Hz) selected automatically, 100/240 V, 50/60 Hz</li><li>Includes: GENESYS 50 Spectrophotometer</li><li>Keypad: Touchscreen</li><li>Lamp Life: &gt; 5 years typical, 3 years guaranteed</li><li>Noise: (RMS at 500 nm 60 consecutive measurements)<ul><li>≤0.00020A at 0A at 260 and 500 nm</li><li>≤0.00030A at 1A at 260 and 500 nm</li><li>≤0.00040A at 2A at 260 and 500 nm</li></ul></li><li>Optical Design: Dual Beam</li><li>Photometric Accuracy: ±0.002A at 0.5A, ±0.004A at 1.0A, ±0.008 A at 2.0 A</li><li>Photometric Display: -3A to +5A</li><li>Photometric Range: –2A to +3.5A</li><li>Photometric Repeatability: ±0.001 A at 1 A measured at 1.0 A at 546 nm</li><li>Printer Included: Snap-on printer available</li><li>Scan Speed: Slow, medium and fast – up to 1600 nm/min.</li><li>Stray Light: &lt; 1.0%T 198 nm (KCl) , &lt;0.05%T at 220 nm (NaI), &lt;0.03%T at 340 nm (NaNO2)</li><li>Warranty: 2 yrs std + 1 yr ext upon reg</li><li>Wavelength Accuracy: ±0.5 nm</li><li>Wavelength Data Interval: 0.2 nm, 0.5 nm, 1 nm, 2 nm, 5 nm</li><li>Wavelength Repeatability: &lt; ±0.2 nm</li><li>Weight (Metric): 7.5 kg</li><li>Detector Type: Dual Silicon Photodiodes</li><li>Display: 7 in. High Definition Color Touchscreen, Fixed, 800 x 1280 pixels</li><li>Spectral Bandwidth: 2 nm</li><li>Wavelength Range: 190 to 1100 nm</li><li>Unit Size: Each</li></ul>

Thermo Scientific™ GENESYS™ 150 UV-Vis Spectrophotometer

Perform quantitative measurements in routine industrial QA/QC or university chemistry labs with the automated and network-capable Thermo Scientific™ GENESYS 150 UV-Vis Spectrophotometer.

<h4>GENESYS - developed for users, designed for results</h4>Built on the foundation of 60 years of spectroscopy experience, the next generation Thermo Scientific™ GENESYS™ family of Vis/UV-Vis spectrophotometers provides exceptional performance with a contemporary design.<ul><li>Start taking measurements immediately with the simple-to-use, high-resolution, color-display start menu</li><li>Exchange samples with ease with large, accessible sample compartment</li><li>Save your data fast any way you want it with multiple data-export options</li></ul>There is a GENESYS spectrophotometer you can count on to provide accurate, reproducible results for your application requirements.<h3>GENESYS 150 UV-Vis Spectrophotometer</h3>For advanced teaching labs, R&amp;D, research and life science applications requiring higher throughput or temperature control.<h5>Elite performance, enhanced capabilities</h5><ul><li>7-inch, high-resolution touchscreen interface</li><li>Local control increases speed and reliability for routine analysis</li><li>Large sample compartment with front access simplifies sample handling</li><li>Wi-Fi ready - save data and methods, print results from on-board control</li></ul><h5>Designed for</h5><ul><li>Chemistry labs</li><li>Routine measurements</li><li>Moderate throughput requirements</li></ul><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Pragolab_Thermo_Scientific_vis_spektrofotometr_genesys_150_titul_B_a202c02cea.jpg" alt="Thermo Scientific™ GENESYS™ 150 Uv-Vis Spectrophotometer" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Pragolab_Thermo_Scientific_vis_spektrofotometr_genesys_150_titul_B_a202c02cea.jpg 180w," sizes="100vw" width="415" height="360"><h3>Walk-up convenience</h3>Simple-to-use, touchscreen user interface features the methods and applications most important to you.<img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Thermo_Scientific_Genesys_spectrophotometers_ae282fe726.jpg" alt="Thermo Scientific: Genesys spectrophotometers" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Thermo_Scientific_Genesys_spectrophotometers_ae282fe726.jpg 239w," sizes="100vw" width="1422" height="928"><h5>Flexible computer control</h5>Optional VISIONlite software offers complete control from a Microsoft ® Windows ® computer providing applications for:<ul><li>Scanning</li><li>Quantitative analysis</li><li>Kinetics</li><li>Fixed wavelength measurements</li></ul><h3>GENESYS 150 UV-Vis Spectrophotometer Specifications</h3><ul><li>Baseline Flatness: ±0.002 A</li><li>Compartments: Accommodates (optional):<ul><li>8-position cell changer</li><li>4-position cell changer (long path cells)</li><li>Peltier thermostatted cell holder (20–60 °C)</li><li>Sipper accessory</li><li>Fiber optic probe coupler</li></ul></li><li>Connections:&nbsp;<ul><li>Single USB-A</li><li>Duplex USB-A</li><li>Export data to network or PC via USB, Ethernet, or Wi-Fi USB adaptor, available</li><li>Print via USB, Ethernet or Wi-Fi USB adaptor available</li></ul></li><li>Dimensions (L x W x H): 35.5 x 38.5 x 19.5 cm</li><li>Drift: (At 500 nm after 1 hour warmup) &lt;0.0005 A/hr.</li><li>Electrical Requirements: External AC to DC converter. Voltage and Frequency (Hz) selected automatically, 100/240 V, 50/60 Hz</li><li>Includes: Genesys 150 Spectrophotometer</li><li>Keypad: Touchscreen</li><li>Lamp Life: &gt; 5 years typical, 3 years guaranteed</li><li>Noise: (RMS at 500 nm 60 consecutive measurements)<ul><li>≤0.00020A at 0A at 260 and 500 nm</li><li>≤0.00030A at 1A at 260 and 500 nm</li><li>≤0.00040A at 2A at 260 and 500 nm</li></ul></li><li>Optical Design: Dual Beam</li><li>Photometric Accuracy: ±0.002A at 0.5A, ±0.004A at 1.0A, ±0.008 A at 2.0 A</li><li>Photometric Display: -3A to +5A</li><li>Photometric Range: –2A to +3.5A</li><li>Photometric Repeatability: ±0.001 A at 1 A measured at 1.0 A at 546 nm</li><li>Printer Included: Snap-on printer available</li><li>Scan Speed: Slow, medium and fast – up to 1600 nm/min.</li><li>Stray Light: &lt; 1.0%T 198 nm (KCl) , &lt;0.05%T at 220 nm (NaI), &lt;0.03%T at 340 nm (NaNO2)</li><li>Warranty: 2 yrs std + 1 yr ext upon reg</li><li>Wavelength Accuracy: ±0.5 nm</li><li>Wavelength Data Interval: 0.2 nm, 0.5 nm, 1 nm, 2 nm, 5 nm</li><li>Wavelength Repeatability: &lt; ±0.2 nm</li><li>Weight (Metric): 7.5 kg</li><li>Detector Type: Dual Silicon Photodiodes</li><li>Display: 7 in. High Definition Color Touchscreen, Tiltable, 800 x 1280 pixels</li><li>Spectral Bandwidth: 2 nm</li><li>Wavelength Range: 190 to 1100 nm</li><li>Unit Size: Each</li></ul>

Thermo Scientific™ GENESYS™ 180 UV-Vis Spectrophotometer

Perform high throughput quantitative UV-Vis measurements where a double beam is required as a reference cell position with the  Thermo Scientific™ GENESYS™ 180 UV-Visible Spectrophotometer. 

<h4>GENESYS - developed for users, designed for results</h4>Built on the foundation of 60 years of spectroscopy experience, the next generation Thermo Scientific™ GENESYS™ family of Vis/UV-Vis spectrophotometers provides exceptional performance with a contemporary design.<ul><li>Start taking measurements immediately with the simple-to-use, high-resolution, color-display start menu</li><li>Exchange samples with ease with large, accessible sample compartment</li><li>Save your data fast any way you want it with multiple data-export options</li></ul>There is a GENESYS spectrophotometer you can count on to provide accurate, reproducible results for your application requirements.<h3>GENESYS 180 UV-Vis Spectrophotometer</h3>For laboratories requiring double beam with a reference cell position.<h5>High-throughput ready</h5><ul><li>Double-beam optics</li><li>Ideal solution when a reference beam is required, such as for kinetics</li><li>8-cell changer standard</li><li>Make measurements outside the sample compartment without cuvettes using fiber optic coupler and probe</li></ul>Designed for laboratories requiring double beam operation with a reference cell position.<img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Pragolab_Thermo_Scientific_vis_spektrofotometr_genesys_180_titul_D_open_90df06abb8.jpg" alt="Thermo Scientific™ GENESYS™ 180 UV-Vis Spectrophotometer" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Pragolab_Thermo_Scientific_vis_spektrofotometr_genesys_180_titul_D_open_90df06abb8.jpg 171w," sizes="100vw" width="467" height="427"><h3>Walk-up convenience</h3>Simple-to-use, touchscreen user interface features the methods and applications most important to you.<img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Thermo_Scientific_Genesys_spectrophotometers_ae282fe726.jpg" alt="Thermo Scientific Genesys spectrophotometers" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Thermo_Scientific_Genesys_spectrophotometers_ae282fe726.jpg 239w," sizes="100vw" width="1422" height="928"><h5>Flexible computer control</h5>Optional VISIONlite software offers complete control from a Microsoft ® Windows ® computer providing applications for:<ul><li>Scanning</li><li>Quantitative analysis</li><li>Kinetics</li><li>Fixed wavelength measurements</li></ul><h3>GENESYS 180 UV-Vis Spectrophotometer Specifications</h3><ul><li>Baseline Flatness: ±0.002 A</li><li>Compartments:<ul><li>8-position cell changer (standard)</li><li>Also accommodates (optional):<ul><li>4-position cell changer (long path cells)</li><li>Peltier thermostatted cell holder (20–60 °C)</li><li>Sipper accessory</li><li>Fiber optic probe coupler</li></ul></li></ul></li><li>Connections:&nbsp;<ul><li>Single USB-A</li><li>Duplex USB-A</li><li>Export data to network or PC via USB, Ethernet, or Wi-Fi USB adaptor, available</li><li>Print via USB, Ethernet or Wi-Fi USB adaptor available</li></ul></li><li>Dimensions (L x W x H): 35.5 x 38.5 x 19.5 cm</li><li>Drift: (At 500 nm after 1 hour warmup) &lt;0.0005 A/hr.</li><li>Electrical Requirements: External AC to DC converter. Voltage and Frequency (Hz) selected automatically, 100/240 V, 50/60 Hz</li><li>Keypad: Touchscreen</li><li>Lamp Life: &gt; 5 years typical, 3 years guaranteed</li><li>Noise: (RMS at 500 nm 60 consecutive measurements)<ul><li>≤0.00020A at 0A at 260 and 500 nm</li><li>≤0.00030A at 1A at 260 and 500 nm</li><li>≤0.00040A at 2A at 260 and 500 nm</li></ul></li><li>Optical Design: Double Beam</li><li>Photometric Accuracy: ±0.002A at 0.5A, ±0.004A at 1.0A, ±0.008 A at 2.0 A</li><li>Photometric Display: -3A to +5A</li><li>Photometric Range: –2A to +3.5A</li><li>Photometric Repeatability: ±0.001 A at 1 A measured at 1.0 A at 546 nm</li><li>Printer Included: Snap-on printer available</li><li>Scan Speed: Slow, medium and fast – up to 1600 nm/min.</li><li>Stray Light: &lt; 1.0%T 198 nm (KCl) , &lt;0.05%T at 220 nm (NaI), &lt;0.03%T at 340 nm (NaNO2)</li><li>Warranty: 2 yrs std + 1 yr ext upon reg</li><li>Wavelength Accuracy: ±0.5 nm</li><li>Wavelength Data Interval: 0.1 nm, 0.2 nm, 0.5 nm, 1 nm, 2 nm, 5 nm</li><li>Wavelength Repeatability: &lt; ±0.2 nm</li><li>Weight (Metric): 7.5 kg</li><li>Detector Type: Dual Silicon Photodiodes</li><li>Display: 7 in. High Definition Color Touchscreen, Tiltable, 800 x 1280 pixels</li><li>Spectral Bandwidth: 2 nm</li><li>Type: UV-Vis Spectrophotometer</li><li>Wavelength Range: 190 to 1100 nm</li><li>Unit Size: Each</li></ul>

Thermo Scientific BioMate 160 UV-Vis Spectrophotometer

Perform quantitative measurements in life science laboratories with the automated and network-capable Thermo Scientific™ BioMate™ 160 UV-Visible Spectrophotometer. 

Perform quantitative measurements in life science laboratories with the automated and network-capable Thermo Scientific™ BioMate™ 160 UV-Visible Spectrophotometer. The BioMate 160 Spectrophotometer offers pre-programmed assay methods for nucleic acid and protein concentration as well as cell culture measurements. Optimized for usability and performance, this platform features a high-resolution color touchscreen, optional Wi-Fi networking, and a rugged design.The BioMate UV-Vis Spectrophotometer provides automation for high-throughput options and room-light resistance, allowing lid-open operation for improved speed and convenience. Software includes pre-programmed life science methods such as Nucleic Acid A260; Protein A280 and A205; Protein Colorimetric assays and OD600.A full line of accessories, including automated cell changers and cell holders, sipper, and fiber optic probes are designed to simplify sampling and accommodate high throughput measurements and temperature control.<h5>Features:</h5><ul><li>7-inch high-resolution touchscreen user interface</li><li>Pre-programmed life science methods</li><li>Networking capable (Wi-Fi adaptor available)</li><li>Room light resistance for 'open-lid' operation</li><li>Sipper accessory</li><li>Removable, washable sample compartment liner with magnetic placement and hold-down</li><li>Accommodates cells up to 100 mm pathlength</li><li>(Optional) 8-position cell changer with switchable turret</li><li>(Optional) 4-position cell changer (long path cells)</li><li>(Optional) Peltier-thermostatted single cell holder (20 °C - 60 °C)</li></ul><h5>Designed for:</h5><ul><li>Advanced teaching labs</li><li>R&amp;D departments</li><li>Applications requiring high throughput or temperature control</li></ul><h4>Specifications</h4>Instrument Type: UV-Visible Spectrophotometer Optical Design: Dual beam Wavelength Range: 190–1100 nm Spectral Bandwidth: 2 nm Photometric Range: –2 A to +3.5 A Photometric Accuracy: ±0.002 A at 0.5 A, ±0.004 A at 1.0 A, ±0.008 A at 2.0 A Photometric Repeatability: ±0.001 A at 1 A (546 nm) Photometric Display: –3 A to +5 A Noise (RMS at 500 nm):<ul><li>≤0.00020 A at 0 A</li><li>≤0.00030 A at 1 A</li><li>≤0.00040 A at 2 A Drift: &lt;0.0005 A/hr at 500 nm after 1-hour warmup Baseline Flatness: ±0.002 A Stray Light:</li><li>&lt;1.0%T at 198 nm (KCl)</li><li>&lt;0.05%T at 220 nm (NaI)</li><li>&lt;0.03%T at 340 nm (NaNO₂)</li></ul><h5>Wavelength Performance</h5><ul><li>Accuracy: ±0.5 nm</li><li>Repeatability: ±0.2 nm</li><li>Data Interval Options: 0.2, 0.5, 1, 2, 5 nm</li><li>Scan Speed: Slow, medium, fast (up to 1600 nm/min)</li></ul><h5>Display &amp; User Interface</h5><ul><li>Touchscreen: 7 in. high-definition color, tiltable (800 × 1280 px)</li><li>Keypad: Touchscreen interface</li></ul><h5>Connectivity &amp; Data Handling</h5><ul><li>Connections: Single USB-A, Duplex USB-A</li><li>Data Export: USB, Ethernet, Wi-Fi adaptor (optional)</li><li>Printer Support: Snap-on printer (optional), USB/Ethernet/Wi-Fi printing available</li></ul><h5>Sample Compartment Compatibility (Optional Accessories)</h5><ul><li>8-position or 4-position cell changers</li><li>Long path cell changer</li><li>Peltier thermostatted cell holder (20–60 °C)</li><li>Sipper accessory</li><li>Fiber optic probe coupler</li></ul><h5>General Specifications</h5><ul><li>Lamp Life: &gt;5 years typical, 3 years guaranteed</li><li>Detector Type: Dual silicon photodiodes</li><li>Dimensions (L × W × H): 35.5 × 38.5 × 19.5 cm</li><li>Weight: 7.5 kg</li><li>Power Requirements: 100–240 V, 50/60 Hz (auto-selecting via external AC–DC converter)</li><li>Certifications: Certificate of Analysis (COA), Certificate of Conformance (COC)</li><li>Warranty: 2 years standard + 1-year extension upon registration</li><li>Unit Size: Each</li></ul>

Products focused on UV–VIS spectrophotometry from Thermo Fisher Scientific - page 1

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