<div>Purifying the target protein is crucial for investigating its specific properties and interactions with biomolecules like DNA, RNA, and other proteins. A range of purification strategies is available, and the optimal approach depends on factors such as the required scale, throughput, and downstream application. In many cases, the process must be fine-tuned through empirical optimization to achieve the desired purity and yield.</div><div><div><div><div><h3>What is Protein Purification?</h3>Protein purification is a critical process in biotechnology and life sciences, aimed at isolating a specific protein from complex biological mixtures such as cell lysates or tissue extracts. The goal is to obtain a highly purified protein sample, free from contaminants like other proteins, nucleic acids, and lipids, to enable accurate analysis of its structure, function, and interactions.This process is especially vital in biotechnological areas inside immunology and microbiology. In immunology, it underpins the production of vaccines, therapeutic antibodies, and diagnostic assays by enabling the isolation of key immune proteins and antibodies. It also supports the investigation of cell signaling and immune response mechanisms. In microbiology, purified proteins are essential for studying pathogens, identifying disease-related antigens, and driving drug discovery efforts. Ultimately, protein purification is fundamental to the development of targeted vaccines, innovative diagnostics, and effective therapeutic strategies.</div></div></div></div><div><div><div><div><h3>Types of Protein Purification Methods</h3></div></div></div></div><div>There are several types of protein purification methods, each chosen based on the protein’s characteristics such as size, charge, solubility, and binding affinity. Below are some commonly used methods:</div><div><div><div><div><h4>Non-Chromatographic Approaches</h4></div></div><div>Non-chromatographic approaches use simpler physicochemical principles—such as solubility, molecular size, or diffusional properties—to separate and purify proteins. These techniques are often employed as initial purification or concentration steps before further refinement.<h5>Precipitation Techniques</h5>Protein precipitation is a widely used approach in purification workflows, typically relying on one of three main methods:<ul><li>Organic solvent precipitation involves adding solvents like ethanol or acetone to lower the solution's dielectric constant. This promotes protein–protein interactions, reducing solubility and leading to aggregation and precipitation.</li><li>Isoelectric point precipitation exploits the pH at which a protein carries no net charge. At this point, solubility is minimized, causing the protein to precipitate from solution.</li><li>Nonionic polymer precipitation uses polymers such as dextran, polyethylene glycol (PEG), or NPEP to alter the solution’s physical properties. These agents create crowding effects that promote protein aggregation without directly binding to the proteins.</li></ul><h5>Dialysis</h5>Dialysis involves placing a protein solution inside a semi-permeable membrane that allows water and ions to pass while the protein remains inside, facilitating buffer exchange.This method is commonly used during protein expression and purification workflows for buffer exchange, desalting, and removal of unwanted small molecules such as imidazole, urea, or ammonium sulfate. The process helps maintain protein stability and activity by gradually equilibrating the sample with the desired buffer conditions.<h5>Immunocapture (Magnetic Bead-Based Purification</h5>Immunocapture uses antibodies immobilized on solid supports like magnetic beads to selectively bind and isolate a target protein. This method is highly specific and particularly useful for low-abundance targets or small-scale prep, such as:<ul><li>Pulling down proteins from complex mixtures</li><li>Capturing tagged recombinant proteins (e.g., FLAG, HA, or His tags)</li><li>Enrichment before western blotting, MS, or ELISA</li></ul>Magnetic beads offer speed, ease of use, and minimal sample loss, making them ideal for lab-scale workflows.<a target="_blank" rel="noopener noreferrer" href="https://www.phenomenex.com/products/biozenspe-sample-preparation-products/biozen-magbeads">Biozen MagBeads</a> provide a fast and efficient method for purifying, isolating, and cleaning up monoclonal antibodies (mAbs), proteins, and peptides. These paramagnetic beads feature a streptavidin-coated surface, enabling high-affinity binding in immuno-based workflows. Compared to traditional sample preparation techniques, magnetic bead-based immunocapture offers greater speed and consistency by delivering high binding capacity with uniform particle size, ensuring accurate and reproducible results in significantly less time.<h5>Centrifugation</h5>Centrifugation of crude protein mixtures is an essential step in the investigation of protein purification and aggregation. Outlined below are the various types of centrifugations employed in protein purification.Preparative Ultracentrifugation Ultracentrifugation is an effective method of purifying proteins from aggregates based on their size, shape and density. Centrifugation, widely recognized as a robust and versatile method, is commonly used to separate complex mixtures of cellular components. Ultracentrifuges separate small particles by spinning at high speeds, utilizing centrifugal force based on size and density.Differential Centrifugation Differential centrifugation operates by separating components based on differences in size and density, using varying speeds of centrifugation to achieve this. Its resolving power ranges from low to medium. A key advantage of this technique is its convenience for processing multiple samples simultaneously, making it effective for purifying crude samples. Additionally, it preserves cell viability and maintains the natural distribution of hematopoietic cell populations.Rate-zonal Gradient Centrifugation This technique separates components based on their sedimentation rate within a medium that contains a density gradient. It offers medium resolving power and is particularly effective for separating multiple components in complex mixtures with high precision. It is also considered a cost-effective and widely used technique in molecular biology and biochemistry.Isopycnic Centrifugation (Equilibrium Centrifugation) Isopycnic centrifugation is an advanced protein purification method that relies on the principle of separating particles according to their intrinsic buoyant density, irrespective of their size or morphology. During the process, proteins migrate through a density gradient until they equilibrate at a position where their density matches that of the surrounding medium, resulting in precise separation without further sedimentation.</div></div></div><div><div><div><div><h4>Chromatographic Approaches</h4>Chromatography is a common method of protein purification and is based on the separation of molecules in a mixture as they move through a stationary phase and a mobile phase. Outlined below are the various types of chromatography employed in protein purification.<h5>Ion Exchange Chromatography</h5><a target="_blank" rel="noopener noreferrer" href="https://www.phenomenex.com/techniques/hplc-ion-exchange">Ion exchange chromatography</a> relies on the interaction between a charged stationary phase and oppositely charged analytes, with cation exchange chromatography attracting positively charged molecules to a negatively charged stationary phase. Successful application of this technique requires careful selection of the appropriate ion exchange column, buffer pH, and salt concentration. This approach is often used in the biopharmaceutical industries as mAb polishing steps, viral clearance and charge variant analyses as part of the product quality control.<a target="_blank" rel="noopener noreferrer" href="https://www.phenomenex.com/">Phenomenex</a> offers a range of protein purification columns. Their ion exchange chromatography solutions include:&nbsp;<ul><li><a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/products/1526">Luna SCX </a>for strong selectivity of positively charged compounds, such as peptides</li><li><a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/products/1540">Biozen WCX</a> for efficient separation of proteins such as monoclonal antibodies charged variants</li><li><a target="_blank" rel="noopener noreferrer" href="https://www.phenomenex.com/part/00g-3151-e0">PhenoSphere SAX</a>, a strong anion-exchanger with fully porous 80Å particles, particularly useful for small molecules such as nucleotides</li></ul><h5>Size Exclusion Chromatography</h5><a target="_blank" rel="noopener noreferrer" href="https://www.phenomenex.com/techniques/hplc-sec">Size exclusion chromatography (SEC)</a> separates molecules based on their size, with larger analytes eluting first due to their exclusion from the pores in the column packing material, while smaller molecules are able to enter the pores, facing a longer travelling path, and hence, eluting later. This method can be categorized into aqueous SEC (gel filtration chromatography, GFC) for analyzing biomolecules such as proteins and antibodies. This type of chromatography is widely used as an analytical tool for quality control in the biopharmaceutical industry and can also be an approach of choice for polishing steps.Phenomenex offers a range of Biozen columns for SEC separations. In the case of mAbs, <a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/products/1534">Biozen dSEC-2</a> and<a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/products/1537"> Biozen dSEC-7 </a>columns are the top choices for monoclonal antibody (mAb) analysis, delivering high-resolution separation of monomers, aggregates, and fragments formed during mAb manufacturing, processing, or storage. The dSEC-7 column, featuring a larger pore size and optimized particle design, extends the dynamic range to accommodate larger biomolecules and complex biotherapeutics.For more general applications, <a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/products/1533">Biozen SEC-2</a> and <a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/products/1532">Biozen SEC-3</a> phases cover different MW ranges for high-efficiency separation of biomolecules (e.g., proteins and antibodies). Additionally, the BioSep and Yarra lines become top choices when looking for preparative scale columns.<h5>Reversed-Phase Chromatography</h5><a target="_blank" rel="noopener noreferrer" href="https://www.phenomenex.com/techniques/hplc-reversed-phase">Reversed-phase chromatography</a>, the most common mode in high-performance liquid chromatography/ ultra-high-performance liquid chromatography (HPLC/UHPLC), separates compounds based on hydrophobic and van der Waals interactions using alkyl (C18, C8, C4) stationary phases or other hydrophobic polymers. For large biomolecules such as proteins and peptides, <a target="_blank" rel="noopener noreferrer" href="https://www.phenomenex.com/resources/blog/wider-pore-biozen-column">wide-pore</a> (typically 300 Å) C4, C8 and C18 columns are ideal, as the larger pore size allows better accessibility of the protein molecules to the stationary phase surface, improving mass transfer and peak shape during separation.The Biozen line from Phenomenex offers a wide range of HPLC/UHPLC columns for the analysis of biomolecules.These include phases optimized for high-resolution analysis of intact proteins (Native-RP, Widepore C4, Intact XB-C8) and peptides (Peptide PS-C18, Peptide XB-C18 and Peptide Polar-C18). Kinetex could also be an option when looking for more selectivities for the analysis of peptides and small proteins. Additionally, when aiming to preparative scale processes, several of these media are also available in Phenomenex Axia formats to easily up-scale your separations.<h5>Hydroxyapatite Chromatography</h5>Hydroxyapatite chromatography (HAC) is a sophisticated mixed-mode chromatographic technique that exploits the unique properties of hydroxyapatite (Ca10(PO4)6(OH)2), utilizing both cation-exchange interactions via its phosphate groups and metal affinity through its calcium sites to achieve highly selective protein separations.Renowned for its efficacy in resolving complex biomolecular separations, HAC excels in tasks like aggregate removal from monoclonal antibodies, surpassing the capabilities of traditional single-mode chromatographic methods such as ion-exchange or hydrophobic interaction chromatography.<h5>Hydrophobic Interaction Chromatography</h5>Hydrophobic interaction chromatography (HIC) is a method of protein purification that separates molecules based on their hydrophobicity, using high-salt buffers to promote protein-ligand interactions while maintaining the protein's biological activity. The process involves gradually reducing the salt concentration to elute proteins in order to increase hydrophobicity, making it effective for purifying target molecules and removing impurities or aggregates, making it a valuable approach for intermediate and polishing steps during purification processes.<h5>Affinity Chromatography</h5>Affinity chromatography is a powerful and highly selective technique used to purify biomolecules based on specific, reversible interactions between a target molecule and an immobilized ligand. In many cases, recombinant proteins are engineered with affinity tags—such as His-tags or GST-tags—that facilitate binding to complementary ligands on the chromatography matrix.This method offers the advantage of achieving several thousand-fold purifications in a single step while preserving the biological activity of the protein. It is widely employed for purifying enzymes, antibodies, and antigens, using a variety of ligands including specific antibodies, enzymes, and multifunctional compounds like concanavalin A and triazine dyes.</div></div></div></div>

Exploring Protein Purification Methods: Essential Techniques for Accurate Results

<h3>A Practical Guide to Getting the Most from Syringe Filters: Tips, Tricks, and Common Pitfalls</h3>Syringe filters play a crucial role in chromatography and many other laboratory applications by removing particulate matter before analysis. Proper sample filtration helps protect analytical instruments, extends column lifetime, and improves data quality. However, selecting an unsuitable filter—or using one incorrectly—can introduce contamination, reduce filtration efficiency, or even compromise analytical results. The following recommendations will help you avoid common mistakes and ensure reliable filtration every time.<img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/KNAUER_Avoiding_Common_Syringe_Filter_Mistakes_3_150e8c1c2b.webp" alt="KNAUER: Avoiding Common Syringe Filter Mistakes" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_KNAUER_Avoiding_Common_Syringe_Filter_Mistakes_3_150e8c1c2b.webp 133w," sizes="100vw" width="1276" height="1496"><h4>1. Improper Filter Handling</h4>Using excessive force during filtration or attempting to reuse disposable syringe filters can damage the membrane and increase the risk of sample contamination.<ul><li>Solution: Apply slow, even pressure throughout the filtration process and always dispose of single-use filters after one use. If the flow rate decreases noticeably, replace the filter instead of applying additional force.</li></ul><h4>2. Selecting an Inappropriate Pore Size</h4>Using the wrong pore size may either fail to remove unwanted particles or cause the membrane to clog prematurely, reducing filtration efficiency.<ul><li>Solution: A 0.45 µm membrane is generally suitable for routine particulate removal, while a 0.22 µm membrane is recommended for finer filtration and bacterial removal. Samples with higher viscosity may require a pre-filter to prevent rapid clogging. It is also important to consider the intended analytical technique. For example, 0.22 µm filters are typically preferred for UHPLC and LC-MS applications, whereas 0.45 µm filters are often sufficient for conventional HPLC analyses.</li></ul><h4>3. Choosing the Wrong Membrane Material</h4>Different membrane materials exhibit different chemical resistance. Using an incompatible membrane may lead to degradation of the filter, sample contamination, or complete filtration failure.<ul><li>Solution: Always verify solvent compatibility before filtration. PTFE membranes are an excellent choice for organic solvents, while PES and nylon membranes are better suited to aqueous samples. If compatibility is uncertain, perform a small-scale trial before filtering valuable samples.</li><li>Pro Tip: For additional guidance, see our blog article "How to Choose the Right Membrane for your Syringe Filter", which provides a detailed comparison of membrane materials and their recommended applications.</li></ul><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/KNAUER_Avoiding_Common_Syringe_Filter_Mistakes_2_e47021fb11.jpg" alt="KNAUER Sepapure® Syringe Filters are available with multiple membrane materials, each identified by a dedicated color code for quick and reliable selection." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_KNAUER_Avoiding_Common_Syringe_Filter_Mistakes_2_e47021fb11.jpg 245w," sizes="100vw" width="1580" height="902"><h4>4. Ignoring Filter Capacity</h4>Using a filter with insufficient capacity can result in rapid membrane blockage, increasing filtration time and reducing laboratory productivity.<ul><li>Solution: When filtering larger sample volumes, choose high-capacity syringe filters or introduce a pre-filtration step. Never force liquid through a clogged membrane, as doing so may damage the filter and negatively affect sample quality.</li></ul><h4>5. Overlooking Sample Adsorption</h4>Certain membrane materials may adsorb analytes of interest, leading to lower recoveries and potentially affecting quantitative results.<ul><li>Solution: For protein samples or other adsorption-sensitive analytes, select membranes with low binding characteristics, such as nylon or glass fiber, whenever appropriate. Performing a small recovery test before routine analysis can help identify potential analyte losses.</li></ul><h4>6. Failing to Optimize Filtration Performance</h4>Slow filtration is more than just an inconvenience—it often indicates that the filtration conditions are not optimal.<ul><li>Solution: If filtration becomes unusually slow, investigate possible causes such as membrane clogging, excessive sample viscosity, or an unnecessarily small pore size. Depending on the sample, dilution or sequential filtration using progressively finer filters may significantly improve performance.</li></ul><h4>Final Thoughts</h4>Selecting the appropriate syringe filter is a straightforward process, but overlooking small details can have a significant impact on analytical performance. Choosing the correct membrane material, pore size, and filtration strategy helps improve laboratory efficiency, protects chromatography systems and columns, and contributes to accurate, reproducible analytical results.With the right syringe filter and proper filtration technique, you can minimize sample preparation problems and maximize confidence in every analysis.<img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/KNAUER_Avoiding_Common_Syringe_Filter_Mistakes_4_983a15e289.jpg" alt="KNAUER: Sepapure® Syringe Filter Selection" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_KNAUER_Avoiding_Common_Syringe_Filter_Mistakes_4_983a15e289.jpg 245w," sizes="100vw" width="1160" height="627">Happy filtering!

Avoiding Common Syringe Filter Mistakes

News from LabRulezLCMS Library - Week 27, 2026

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Phenomenex is a global technology leader committed to developing novel analytical chemistry solutions that solve the separation and purification challenges of researchers in industrial, government and academic laboratories.

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Based in Berlin, KNAUER is a medium-sized, owner-managed company that has been serving the sciences since 1962. We develop and manufacture scientific instruments of superior quality for liquid chromatography, including: Analytical HPLC/UHPLC, Preparative HPLC, Fast protein liquid chromatography (FPLC), Multi-column chromatography/Simulated moving bed (SMB) chromatography. High pressure dosing, in-line detection systems, and osmometry are additional business areas.

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Thermo Scientific SII for Empower Software

Incorporate and operate Thermo Scientific UHPLC, HPLC, GC, and IC instruments within other vendors' software platforms with the Thermo Scientific™ SII for Empower 3 Software plug-in.

<div id="product-description">Incorporate and operate Thermo Scientific UHPLC, HPLC, GC, and IC instruments within other vendors' software platforms with the Thermo Scientific™ Standard Instrument Integration (SII) for Empower 3 Software plug-in. The SII for Empower 3 plug-in is based on the <a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/products/76">Thermo Scientific™ Chromeleon™ Chromatography Data System (CDS)</a> and offers unique and smart features that deliver maximum ease-of-use, reliability, and performance. It also enables Thermo Scientific™ chromatography systems to be operated and controlled by <a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/products/627">Waters™ Empower™ 3 CDS software</a>.</div><div>While Chromeleon CDS is the recommended software for control of Thermo Scientific UHPLC, HPLC, GC, and IC instruments due to its maximum ease of use, reliability, and performance, other vendors' CDS software can also incorporate and operate these instruments with the SII for Empower™ 3 Software plug-in.The SII for Empower™ Software installs and uses portions of <a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/products/76">Chromeleon CDS</a>, such as the Services Manager, Instrument Configuration Manager, Instrument View, and Instrument Method Editor, to deliver flawless integration of Thermo Scientific LC, IC, and GC instrumentation into Empower™ 3 CDS without loss of functionality. Instrument control, data acquisition, and data processing are performed completely under Empower™ 3 Software.Configure your instruments Up to four instruments can be configured per Empower™ node using the standard Chromeleon Instrument Configuration Manager.Control your instruments SII for Empower™ Software provides a complete set of commands because they are present under native Chromeleon CDS control. For the user, this means that all the Chromeleon CDS tools and advanced features, such as preparing the next sample during an ongoing analysis, can be implemented to increase productivity and receive more data with fewer injections.SII for Empower™ controls the following systems:<ul><li><a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/products?category=37&amp;manufacturerItems=3&amp;search=Vanquish">Thermo Scientific™ Vanquish™ HPLC and UHPLC systems</a></li><li>Thermo Scientific™ UltiMate™ 3000 HPLC systems</li><li><a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/products/410">Thermo Scientific™ Dionex™ ICS-6000 HPIC™ system</a></li><li>Thermo Scientific™ Dionex™ ICS-5000 HPIC™ system</li><li><a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/products/408">Thermo Scientific™ Dionex™ Integrion™ HPIC™ system</a></li><li>Thermo Scientific™ Dionex™ Aquion™ system</li><li>Thermo Scientific™ TRACE™ 1300 GC systems</li></ul>Monitoring instrument status within Empower™ can be performed using the Empower™ Control Panel. Here, an enhanced SII Instrument View is easily accessed to provide further options to control and monitor the instrument and its modules.Instrument Methods Setting up an instrument method is simple, intuitive, and fast due to the SII for Empower™ Instrument Method Wizard and Editor, both of which are integrated into the Instrument Method Editor of Empower™. The wizard guides the user through all modules, enabling the entry of all necessary information, while the editor allows the review and modification of all aspects of a method.Process your data Complete data processing takes place inside Empower™ Software, which allows you to use all functionalities as you would normally, for example, overlay of chromatograms or other channels like pressure traces. The Empower™ user experience does not change for any data processing aspect.</div>

Thermo Scientific LC-MS Data Acquisition Software

Acquire the highest quality data possible to unlock the information, knowledge, and value in your samples with Thermo Scientific LC-MS Data Acquisition Software.

Thermo Scientific™ Chromeleon™ XPS Open Access Software

Thermo Scientific™ Chromeleon™ XPS provides streamlined and easy-to-use interface for Thermo Scientific™ Chromeleon™ CDS, while maintaining full capabilities of the CDS software.

In modern laboratories, chromatography techniques and the use of mass spectrometry (MS) detection are in high demand. Until recently, MS detectors were regarded as sophisticated detection tools that could be operated only by a highly trained specialist. Nowadays mass spectrometers are increasingly used as straightforward detectors for chromatography.Alongside this move into routine use of MS is the continued deskilling of staff in the laboratory world, causing highly experienced chromatographers to be in short supply. The result is a substantial number of regular scientists and process operators that need to generate chromatographic results using advanced instrumentation themselves.The solution is an Open Access tool enabling users to simply walk up and use a very straightforward interface to exploit the full power of the chromatography data system (CDS), without actually using the CDS software itself. Thermo Scientific™ Chromeleon™ XPS provides this streamlined and easy-to-use interface for <a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/products/76">Thermo Scientific™ Chromeleon™ CDS</a>, while maintaining full capabilities of the CDS software, like instrument control, data processing, reporting and compliance tools.<h3>Workflow</h3>Users can walk up to any chromatography instrument connected to the CDS, with or without MS detection, and start to run their analysis with only a few mouse-clicks.<img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Thermo_Scientific_Chromeleon_XPS_Open_Access_Software_5e8f8c3dec.jpg" alt="Thermo Scientific™ Chromeleon™ XPS Open Access Software: First, select the analysis to perform (A) and which instrument should be used (B). The instrument overview shows the current instrument status’ and displays the estimated wait time until each instrument is available and the number of sample positions still available. The instrument list is filtered depending on the analysis selected and the users privileges to only show appropriate instruments." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Thermo_Scientific_Chromeleon_XPS_Open_Access_Software_5e8f8c3dec.jpg 196w," sizes="100vw" width="876" height="697"><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Thermo_Scientific_Chromeleon_XPS_Open_Access_Software_2_a8b64a52ae.jpg" alt="Thermo Scientific™ Chromeleon™ XPS Open Access Software: Next, enter the sample names (C) and any other parameters (D) required for the analysis." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Thermo_Scientific_Chromeleon_XPS_Open_Access_Software_2_a8b64a52ae.jpg 196w," sizes="100vw" width="876" height="697"><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Thermo_Scientific_Chromeleon_XPS_Open_Access_Software_3_4b4367ffae.jpg" alt="Thermo Scientific™ Chromeleon™ XPS Open Access Software: Place the sample vials in the indicated positions and select Submit." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Thermo_Scientific_Chromeleon_XPS_Open_Access_Software_3_4b4367ffae.jpg 196w," sizes="100vw" width="876" height="697"><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Thermo_Scientific_Chromeleon_XPS_Open_Access_Software_4_0386d31c9a.jpg" alt="Thermo Scientific™ Chromeleon™ XPS Open Access Software: The sequence is submitted and will run. The operator is shown the expected start and finish time of the analyses." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Thermo_Scientific_Chromeleon_XPS_Open_Access_Software_4_0386d31c9a.jpg 245w," sizes="100vw" width="625" height="263"><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Thermo_Scientific_Chromeleon_XPS_Open_Access_Software_5_1b51ab8dd2.jpg" alt="Thermo Scientific™ Chromeleon™ XPS Open Access Software: At the end of the run, the results are automatically printed and/or exported and a link to the exported report can be distributed via email." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Thermo_Scientific_Chromeleon_XPS_Open_Access_Software_5_1b51ab8dd2.jpg 245w," sizes="100vw" width="814" height="309"><h3>Configuration and management</h3>Running samples is as easy as described above once Chromeleon XPS software is set up, configured and managed by an experienced chromatographer.<ul><li>All the methods required for the analysis must be created; the instrument method with all instrument control and data acquisition parameters, the processing method with all data processing parameters and the report template with all required results, to be reported.&nbsp;</li><li>A Chromeleon eWorkflow is created using these methods. An eWorkflow is an electronic procedure that automates all steps of the laboratory process related to a chromatographic analysis</li></ul><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Thermo_Scientific_Chromeleon_XPS_Open_Access_Software_6_a3b2219e42.jpg" alt="Thermo Scientific™ Chromeleon™ XPS Open Access Software: The lab manager can customize Chromeleon XPS to show or hide individual toolbar buttons (A), which eWorkflows are visible to users (B) and which instruments they can access (C)." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Thermo_Scientific_Chromeleon_XPS_Open_Access_Software_6_a3b2219e42.jpg 196w," sizes="100vw" width="876" height="697"><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Thermo_Scientific_Chromeleon_XPS_Open_Access_Software_7_d647111f5d.jpg" alt="Thermo Scientific™ Chromeleon™ XPS Open Access Software: In addition, it is possible to define various system options, such as which email notifications to send." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Thermo_Scientific_Chromeleon_XPS_Open_Access_Software_7_d647111f5d.jpg 193w," sizes="100vw" width="659" height="533">Chromeleon XPS Open Access software lets any user run samples on their own by delivering a clean, streamlined walk-up interface that utilizes the full power of Chromeleon CDS. Users can access all instruments controlled by <a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/products/76">Chromeleon CDS</a>, including MS, and automatically receive results or notifications about run completion or failure. The management console contains advanced customization options to fulfill any organization’s requirements, included fully regulated environments.Chromeleon XPS software provides error-free execution of any chromatography analysis with minimal training requirements for users.

Thermo Scientific Vanquish Tandem LC Systems

The Thermo Scientific Vanquish Tandem LC Systems increase throughput and maximize detector output by using a second pump and intelligent column switching to eliminate the time required for column equilibration in a gradient elution. 

<h3>Optimize your productivity</h3>In today’s challenging research and production environments, you are being asked to be more productive without increasing spending or compromising quality.&nbsp;At the same time, advancements and innovations are needed to improve operational efficiency and performance. How do you accomplish this? It’s easy with the Thermo Scientific™ Vanquish™ High-throughput LC systems built for enhanced productivity. Vanquish High-throughput LC systems combine individual Vanquish components and software wizards to deliver increased throughput or detector utilization.<img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Thermo_Scientific_Vanquish_Duo_UHPLC_Systems_8_2befa2ab8e.jpg" alt="Thermo Scientific Vanquish Tandem LC Systems." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Thermo_Scientific_Vanquish_Duo_UHPLC_Systems_8_2befa2ab8e.jpg 191w," sizes="100vw" width="1325" height="1086"><h3>Two flow paths for maximized productivity</h3>Your laboratory may be facing increased pressure to improve working efficiency and deal with limiting resources, such as budget, space, or manpower. Vanquish High-throughput LC systems are designed to overcome these limitations by providing multiple flow paths for parallelization of specific stages of the LC run.<img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Thermo_Scientific_Vanquish_Duo_UHPLC_Systems_2_06370d93f8.jpg" alt="Thermo Scientific Vanquish Tandem LC Systems." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Thermo_Scientific_Vanquish_Duo_UHPLC_Systems_2_06370d93f8.jpg 245w," sizes="100vw" width="955" height="591"><h4>Unique dual technology</h4>The Vanquish platform contains two unique dual modules. Vanquish dual pumps merge two individual, low pressure mixing pumps in one housing enabling separate eluents and gradients for each pump.Vanquish dual split samplers possess two independently controlled injection units including separate injection valves and injection needles. Used in Dual LC and LC-MS workflows only, this enables high throughput and extensive sample characterization workflows.<h3>Two configurations to best serve your needs</h3>Vanquish High-throughput workflows are configured with Thermo Scientific™ Vanquish™ Core system, Thermo Scientific™ Vanquish™ Flex system or Thermo Scientific™ Vanquish™ Horizon system modules. Both workflows make use of two pumps to increase instrument productivity.<img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Thermo_Scientific_Vanquish_Duo_UHPLC_Systems_3_77acff4bea.jpg" alt="Thermo Scientific Vanquish Tandem LC Systems: Two configurations to best serve your needs." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Thermo_Scientific_Vanquish_Duo_UHPLC_Systems_3_77acff4bea.jpg 245w," sizes="100vw" width="1682" height="735"><h4>Vanquish Duo UHPLC Systems</h4><ul><li>Supported systems:<ul><li><a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/products/744">Vanquish Horizon</a></li><li><a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/products/743">Vanquish Flex</a></li></ul></li><li>Sampler: Dual split sampler</li><li>Detector: Two out of the complete detector portfolio</li><li>Software control:<ul><li><a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/products/76">Thermo Scientific™ Chromeleon™ Chromatography Data System (CDS)</a></li><li><a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/products/1838">Thermo Scientific™ Standard Instrument Integration (SII)</a> for <a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/products/627">Waters™ Empower™ 3 CDS</a></li></ul></li></ul><h4>Vanquish Tandem LC Systems</h4><ul><li>Supported systems:<ul><li><a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/products/744">Vanquish Horizon</a></li><li><a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/products/743">Vanquish Flex</a></li><li><a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/products/397">Vanquish Core</a></li></ul></li><li>Sampler: Split sampler</li><li>Detector: One out of the complete detector portfolio and/or one mass spectrometer</li><li>Software control:<ul><li><a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/products/76">Chromeleon CDS</a></li><li>Standard System Integration for <a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/products/1758">Thermo Scientific™ Xcalibur™ software</a></li></ul></li></ul><h3>Vanquish Tandem LC systems</h3><div><div><div><div>Gradient separations are commonly used in LC or LC-MS. Column reconditioning, such as washing and re-equilibration, takes time and reduces your overall sample throughput. The Vanquish Tandem LC systems eliminate these limitations and improve productivity by:<ul><li>Increasing sample throughput without modifying a validated method</li><li>Improving return on investment by maximizing mass spectrometer utilization</li><li>Reducing column carry over through extended column washing without sacrificing throughput</li><li>Simplifying method setup with automatic method conversion from your original gradient method</li></ul></div></div></div></div><div>&nbsp;</div><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Thermo_Scientific_Vanquish_Tandem_LC_Systems_4_a031ad3221.jpg" alt="Thermo Scientific Vanquish Tandem LC Systems." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Thermo_Scientific_Vanquish_Tandem_LC_Systems_4_a031ad3221.jpg 245w," sizes="100vw" width="1749" height="530"><h4>Throughput increase using Tandem LC</h4><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Thermo_Scientific_Vanquish_Tandem_LC_Systems_3_6bc55942ed.jpg" alt="Thermo Scientific Vanquish Tandem LC Systems: Throughput increase by Tandem LC is between 10–80% for most common conditions." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Thermo_Scientific_Vanquish_Tandem_LC_Systems_3_6bc55942ed.jpg 245w," sizes="100vw" width="894" height="434">The Vanquish Tandem LC system splits the analysis between two pumps and two columns. One pump delivers the analytical gradient while the second pump runs the reconditioning gradient. This configuration allows you to use two identical columns to run your sequence faster without compromising data quality.<img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Thermo_Scientific_Vanquish_Tandem_LC_Systems_5_d818007fe4.jpg" alt="Thermo Scientific Vanquish Tandem LC Systems: Reproducible results for the Thermo Scientific™ Vanquish™ Horizon Tandem UHPLC system—Thermo Scientific™ Q Exactive™ HF hybrid quadrupole-Orbitrap™ mass spectrometer showing an overlay of five total ion current chromatograms. The sample shown is digested Infliximab using the Thermo Scientific™ SMART ™ Digest Kit." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Thermo_Scientific_Vanquish_Tandem_LC_Systems_5_d818007fe4.jpg 245w," sizes="100vw" width="1123" height="499"><h4>Simply upgrade your method</h4>Implementing Tandem LC-MS into your workflow is easy with compliance-ready Chromeleon software for LC or LC-MS users and Thermo Scientific Standard Instrument Integration (SII) for Thermo Scientific Xcalibur software for LC-MS users.A simple dedicated instrument method wizard enables you to create new methods without the need for additional training.<img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Thermo_Scientific_Vanquish_Tandem_LC_Systems_0c7f310b1e.jpg" alt="Thermo Scientific Vanquish Tandem LC Systems." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Thermo_Scientific_Vanquish_Tandem_LC_Systems_0c7f310b1e.jpg 193w," sizes="100vw" width="353" height="285"><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Thermo_Scientific_Vanquish_Tandem_LC_Systems_2_47128950b2.jpg" alt="Thermo Scientific Vanquish Tandem LC Systems: Chromeleon CDS and SII for Xcalibur guides users through the implementation of a Tandem LC or LC-MS method with automatic system volume considerations and gradient formation of the reconditioning pump." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Thermo_Scientific_Vanquish_Tandem_LC_Systems_2_47128950b2.jpg 160w," sizes="100vw" width="361" height="353"><h3>Thermo Scientific LC portfolio overview</h3><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Thermo_Scientific_LC_portfolio_overview_2db5044366.jpg" alt="Thermo Scientific LC portfolio overview." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Thermo_Scientific_LC_portfolio_overview_2db5044366.jpg 188w," sizes="100vw" width="795" height="660">

Thermo Scientific Orbitrap Excedion Pro Mass Spectrometers

Welcome to the future of mass spectrometry. Enhance your research and achieve groundbreaking discoveries with Thermo Scientific Orbitrap Excedion Pro Hybrid Mass Spectrometers, the next generation of mass spectrometry.

Thermo Scientific Hypersil GOLD C8 HPLC Columns

For analytes with medium hydrophobicity or when a less hydrophobic phase is required to obtain optimum retention, use Thermo Scientific™ Hypersil GOLD™ C8 LC columns

For analytes with medium hydrophobicity or when a less hydrophobic phase is required to obtain optimum retention, use Thermo Scientific™ Hypersil GOLD™ C8 LC columns for outstanding sensitivity and excellent peak shape. The selectivity is similar to C18 columns with a predictable elution order but less retention. The lower hydrophobicity allows compounds to elute more quickly, yielding faster separations.<h5>Similar selectivity, less retention than C18 columns</h5><ul><li>Successful retention and separation of moderately hydrophobic analytes</li><li>Ideal for fatty acids, which are too hydrophobic for analysis using C18 columns</li></ul><h5>Faster separations</h5><ul><li>Higher analytical throughput compared to C18 alkyl chain chemistry</li><li>Quicker compound elution due to reduced hydrophobic interactions</li></ul><h5>Superior performance</h5><ul><li>Highly symmetrical peak shapes</li><li>Improved speed of analysis, efficiency, and sensitivity</li></ul><h4>Specifications</h4><ul><li>Endcapped: Yes</li><li>Max. Pressure: 5800 psi (400 bar)</li><li>Packing Material: Spherical, Fully Porous, Ultrapure Silica</li><li>Stationary Phase: C8</li><li>Surface Area: 220 m²/g</li><li>Temperature: 60°C</li><li>USP Type: L7</li><li>Carbon Load: 8%</li><li>Phase: Reversed Phase</li><li>Pore Size: 175 Å</li><li>pH: 2 to 9</li></ul><h4>About the Hypersil GOLD Family of HPLC Columns</h4>Meet the challenges of modern chromatography with state-of-the-art columns that provide outstanding performance and consistent separations. Twelve different column chemistries are available so you can optimize separations and maximize productivity. Get outstanding peak shapes in reversed phase, ion exchange, HILIC, or normal phase chromatography.<img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Thermo_Fisher_Scientific_Hypersil_GOLD_Columns_41d5984b62.jpg" alt="Thermo Fisher Scientific: Hypersil GOLD Columns" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Thermo_Fisher_Scientific_Hypersil_GOLD_Columns_41d5984b62.jpg 150w," sizes="100vw" width="747" height="775"><ul><li>Excellent peak symmetry<ul><li>Hypersil GOLD columns produce highly symmetrical peaks, which facilitate the detection of low-level impurities that elute immediately after the main component. When there is even minor tailing, small impurity peaks can elute on the shoulder of the main peak, complicating the integration process.</li></ul></li><li>Narrow peaks for outstanding efficiency</li><li>Enhanced sensitivity and improved resolution</li><li>Exceptional reproducibility for reliable chromatography, column after column</li><li>Solutions for high-throughput screening</li><li>1.9, 3, and 5 μm particle sizes for all your separation needs</li></ul><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Thermo_Fisher_Scientific_Hypersil_GOLD_Peak_symmetry_641b946673.jpg" alt="Thermo Fisher Scientific: Hypersil GOLD Columns: Peak symmetry" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Thermo_Fisher_Scientific_Hypersil_GOLD_Peak_symmetry_641b946673.jpg 149w," sizes="100vw" width="880" height="925">

Thermo Scientific Hypersil GOLD C4 HPLC Columns

For very hydrophobic analytes, achieve faster separations with excellent peak shapes using Thermo Scientific™ Hypersil GOLD™ C4 HPLC columns. 

For very hydrophobic analytes, achieve faster separations with excellent peak shapes using Thermo Scientific™ Hypersil GOLD™ C4 HPLC columns. Featuring a lower hydrophobicity than C18 or C8 columns, Hypersil GOLD C4 columns provide outstanding sensitivity and high efficiency.<h5>Similar selectivity, less retention than C18 or C8 columns</h5><ul><li>Successful retention and separation of very hydrophobic analytes with this short alkyl chain length, low hydrophobicity column</li><li>Ideal for analysis of hydrophobic polypeptides and proteins</li></ul><h5>Superior performance</h5><ul><li>Highly symmetrical peak shapes</li><li>1.9 μm particle size columns improve speed and efficiency</li></ul><h4>Specifications</h4><ul><li>Endcapped: Yes</li><li>Max. Pressure: 18130 psi (1250 bar)</li><li>Packing Material: Spherical, Fully Porous, Ultrapure Silica</li><li>Stationary Phase: C4</li><li>Surface Area: 220 m²/g</li><li>Temperature: 60°C</li><li>USP Type: L26</li><li>Carbon Load: 5%</li><li>Phase: Reversed Phase</li><li>Pore Size: 175 Å</li><li>pH: 2 to 8</li></ul><h4>About the Hypersil GOLD Family of HPLC Columns</h4>Meet the challenges of modern chromatography with state-of-the-art columns that provide outstanding performance and consistent separations. Twelve different column chemistries are available so you can optimize separations and maximize productivity. Get outstanding peak shapes in reversed phase, ion exchange, HILIC, or normal phase chromatography.<img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Thermo_Fisher_Scientific_Hypersil_GOLD_Columns_41d5984b62.jpg" alt="Thermo Fisher Scientific: Hypersil GOLD Columns" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Thermo_Fisher_Scientific_Hypersil_GOLD_Columns_41d5984b62.jpg 150w," sizes="100vw" width="747" height="775"><ul><li>Excellent peak symmetry<ul><li>Hypersil GOLD columns produce highly symmetrical peaks, which facilitate the detection of low-level impurities that elute immediately after the main component. When there is even minor tailing, small impurity peaks can elute on the shoulder of the main peak, complicating the integration process.</li></ul></li><li>Narrow peaks for outstanding efficiency</li><li>Enhanced sensitivity and improved resolution</li><li>Exceptional reproducibility for reliable chromatography, column after column</li><li>Solutions for high-throughput screening</li><li>1.9, 3, and 5 μm particle sizes for all your separation needs</li></ul><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Thermo_Fisher_Scientific_Hypersil_GOLD_Peak_symmetry_641b946673.jpg" alt="Thermo Fisher Scientific: Hypersil GOLD Columns: Peak symmetry" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Thermo_Fisher_Scientific_Hypersil_GOLD_Peak_symmetry_641b946673.jpg 149w," sizes="100vw" width="880" height="925">

Thermo Scientific Hypersil GOLD Phenyl HPLC Columns

Get excellent retention and unique selectivity for aromatic and moderately polar analytes with Thermo Scientific™ Hypersil GOLD™ Phenyl reversed phase HPLC columns

Get excellent retention and unique selectivity for aromatic and moderately polar analytes with Thermo Scientific™ Hypersil GOLD™ Phenyl reversed phase HPLC columns.&nbsp;A butyl (C4) linker in the phenyl bonded phase allows for superior alignment of the phenyl ring with aromatic molecules, thus enhancing pi-pi interactions and improving the retention of the molecules. The C4 linker also provides the stationary phase with moderate hydrophobicity, making it ideal for the separation of analyte mixtures with varying polarity and aromaticity. The outstanding peak shape results in greater sensitivity.<h5>Special selectivity</h5><ul><li>Provides selectivity complementary to that provided by alkyl chain phases</li><li>Moderate hydrophobicity compared to C18</li></ul><h4>Specifications</h4><ul><li>Endcapped: Yes</li><li>Max. Pressure: 5800 psi (400 bar)</li><li>Packing Material: Silica</li><li>Stationary Phase: Phenyl/C4</li><li>Surface Area: 220 m²/g</li><li>Temperature: 60°C</li><li>USP Type: L11</li><li>Carbon Load: 9%</li><li>Phase: Reversed Phase</li><li>Pore Size: 175 Å</li><li>pH: 2 to 8</li></ul><h4>About the Hypersil GOLD Family of HPLC Columns</h4>Meet the challenges of modern chromatography with state-of-the-art columns that provide outstanding performance and consistent separations. Twelve different column chemistries are available so you can optimize separations and maximize productivity. Get outstanding peak shapes in reversed phase, ion exchange, HILIC, or normal phase chromatography.<img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Thermo_Fisher_Scientific_Hypersil_GOLD_Columns_41d5984b62.jpg" alt="Thermo Fisher Scientific: Hypersil GOLD Columns" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Thermo_Fisher_Scientific_Hypersil_GOLD_Columns_41d5984b62.jpg 150w," sizes="100vw" width="747" height="775"><ul><li>Excellent peak symmetry<ul><li>Hypersil GOLD columns produce highly symmetrical peaks, which facilitate the detection of low-level impurities that elute immediately after the main component. When there is even minor tailing, small impurity peaks can elute on the shoulder of the main peak, complicating the integration process.</li></ul></li><li>Narrow peaks for outstanding efficiency</li><li>Enhanced sensitivity and improved resolution</li><li>Exceptional reproducibility for reliable chromatography, column after column</li><li>Solutions for high-throughput screening</li><li>1.9, 3, and 5 μm particle sizes for all your separation needs</li></ul><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Thermo_Fisher_Scientific_Hypersil_GOLD_Peak_symmetry_641b946673.jpg" alt="Thermo Fisher Scientific: Hypersil GOLD Columns: Peak symmetry" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Thermo_Fisher_Scientific_Hypersil_GOLD_Peak_symmetry_641b946673.jpg 149w," sizes="100vw" width="880" height="925">

Thermo Scientific Hypersil GOLD Amino HPLC Columns

Achieve excellent chromatographic separations in weak anion exchange, reversed phase and normal phase modes with Thermo Scient

Achieve excellent chromatographic separations in weak anion exchange, reversed phase and normal phase modes with Thermo Scientific™ Hypersil GOLD™ Amino LC Columns. These columns, featuring a high-performance aminopropyl phase, provide outstanding peak shape and sensitivity and are available with a 1.9μm particle size for high speed, high efficiency separations.<h5>Excellent Chromatographic Properties in Three Modes</h5><ul><li>Retains anions and organic acids in weak anion exchange mode when used with common buffers and an organic modifier</li><li>Excels for carbohydrate analysis in reversed phase mode</li><li>Offers an alternative selectivity to silica columns in normal phase chromatography</li></ul><h4>Specifications</h4><ul><li>Endcapped: Yes</li><li>For Use With: HILIC</li><li>Max. Pressure: 18130 psi (1250 bar)</li><li>Packing Material: Spherical, Fully Porous, Ultrapure Silica</li><li>Stationary Phase: Amino (NH2)</li><li>Surface Area: 220 m²/g</li><li>Temperature: 60°C</li><li>USP Type: L8</li><li>Carbon Load: 2%</li><li>Phase: Reversed Phase, Normal Phase, Ion Exchange, HILIC</li><li>Pore Size: 175 Å</li><li>pH: 2 to 8</li></ul><h4>About the Hypersil GOLD Family of HPLC Columns</h4>Meet the challenges of modern chromatography with state-of-the-art columns that provide outstanding performance and consistent separations. Twelve different column chemistries are available so you can optimize separations and maximize productivity. Get outstanding peak shapes in reversed phase, ion exchange, HILIC, or normal phase chromatography.<img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Thermo_Fisher_Scientific_Hypersil_GOLD_Columns_41d5984b62.jpg" alt="Thermo Fisher Scientific: Hypersil GOLD Columns" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Thermo_Fisher_Scientific_Hypersil_GOLD_Columns_41d5984b62.jpg 150w," sizes="100vw" width="747" height="775"><ul><li>Excellent peak symmetry<ul><li>Hypersil GOLD columns produce highly symmetrical peaks, which facilitate the detection of low-level impurities that elute immediately after the main component. When there is even minor tailing, small impurity peaks can elute on the shoulder of the main peak, complicating the integration process.</li></ul></li><li>Narrow peaks for outstanding efficiency</li><li>Enhanced sensitivity and improved resolution</li><li>Exceptional reproducibility for reliable chromatography, column after column</li><li>Solutions for high-throughput screening</li><li>1.9, 3, and 5 μm particle sizes for all your separation needs</li></ul><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Thermo_Fisher_Scientific_Hypersil_GOLD_Peak_symmetry_641b946673.jpg" alt="Thermo Fisher Scientific: Hypersil GOLD Columns: Peak symmetry" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Thermo_Fisher_Scientific_Hypersil_GOLD_Peak_symmetry_641b946673.jpg 149w," sizes="100vw" width="880" height="925">

Thermo Scientific Hypersil GOLD Silica HPLC Columns

Achieve outstanding peak shape and sensitivity with Thermo Scientific™ Hypersil GOLD™ Silica LC Columns. 

Achieve outstanding peak shape and sensitivity with Thermo Scientific™ Hypersil GOLD™ Silica LC Columns. The unbonded, highly pure, base-deactivated silica media efficiently separates nonpolar and moderately polar organic compounds by normal phase chromatography. Available 1.9μm particle size columns can improve the speed and efficiency of your applications.<h4>Specifications</h4><ul><li>Endcapped: No</li><li>For Use With: Uniguard Direct-Connection and Stand Alone Holder</li><li>Max. Pressure: 5800 psi (400 bar)</li><li>Packing Material: Silica</li><li>Stationary Phase: Silica</li><li>Surface Area: 220 m²/g</li><li>Temperature: 60°C</li><li>USP Type: L3</li><li>Phase: Normal Phase</li><li>Pore Size: 175 Å</li><li>pH: 2 to 8</li></ul><h4>About the Hypersil GOLD Family of HPLC Columns</h4>Meet the challenges of modern chromatography with state-of-the-art columns that provide outstanding performance and consistent separations. Twelve different column chemistries are available so you can optimize separations and maximize productivity. Get outstanding peak shapes in reversed phase, ion exchange, HILIC, or normal phase chromatography.<img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Thermo_Fisher_Scientific_Hypersil_GOLD_Columns_41d5984b62.jpg" alt="Thermo Fisher Scientific: Hypersil GOLD Columns" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Thermo_Fisher_Scientific_Hypersil_GOLD_Columns_41d5984b62.jpg 150w," sizes="100vw" width="747" height="775"><ul><li>Excellent peak symmetry<ul><li>Hypersil GOLD columns produce highly symmetrical peaks, which facilitate the detection of low-level impurities that elute immediately after the main component. When there is even minor tailing, small impurity peaks can elute on the shoulder of the main peak, complicating the integration process.</li></ul></li><li>Narrow peaks for outstanding efficiency</li><li>Enhanced sensitivity and improved resolution</li><li>Exceptional reproducibility for reliable chromatography, column after column</li><li>Solutions for high-throughput screening</li><li>1.9, 3, and 5 μm particle sizes for all your separation needs</li></ul><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Thermo_Fisher_Scientific_Hypersil_GOLD_Peak_symmetry_641b946673.jpg" alt="Thermo Fisher Scientific: Hypersil GOLD Columns: Peak symmetry" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Thermo_Fisher_Scientific_Hypersil_GOLD_Peak_symmetry_641b946673.jpg 149w," sizes="100vw" width="880" height="925">

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