At MSACL 2025, a Thermo Fisher Scientific–sponsored workshop led by Qin Fu addressed one of the most persistent challenges in proteomics: how to efficiently and reproducibly translate biomarker discoveries into clinically meaningful assays. Drawing on experience spanning academia and industry—including prior work in Jennifer Van Eyk’s lab—Fu shared practical insights into building a scalable, end-to-end biomarker discovery-to-validation pipeline.<img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Thermo_Fisher_Scientific_Accelerating_Biomarker_Discovery_and_Validation_From_Discovery_to_Clinical_Utility_9558364cf2.jpg" alt="Thermo Fisher Scientific: Accelerating Biomarker Discovery and Validation: From Discovery to Clinical Utility" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Thermo_Fisher_Scientific_Accelerating_Biomarker_Discovery_and_Validation_From_Discovery_to_Clinical_Utility_9558364cf2.jpg 245w," sizes="100vw" width="1024" height="512">The session highlighted how emerging mass spectrometry technologies, automation, and informatics are reshaping biomarker research—reducing complexity, improving reproducibility, and accelerating the path to clinical utility.<h3>The three tiers of biomarker assays</h3>The workshop opened with a review of the widely accepted three-tier framework that guides biomarker development from early discovery through clinical validation:<h4>Tier 3 – discovery</h4>Exploratory, untargeted workflows such as data-dependent acquisition (DDA) and data-independent acquisition (DIA). These approaches prioritize breadth, enabling the identification of large-magnitude biological changes across hundreds to thousands of analytes, typically with low-to-moderate precision and without internal standards.<h4>Tier 2 – verification</h4>Research-grade targeted assays, including parallel reaction monitoring (PRM) and multiple reaction monitoring (MRM). These methods focus on tens to hundreds of analytes, delivering improved precision and repeatability to confirm trends observed during discovery.<h4>Tier 1 – validation</h4>Clinically validated assays designed to meet rigorous quality and regulatory standards. These workflows incorporate internal standards and strict quality controls to ensure quantitative reproducibility suitable for clinical and translational use.Each tier represents a critical step toward ensuring that biomarker candidates can ultimately deliver reliable patient benefit.<h3>The central challenge: from sample to signal</h3>Reliable biomarker development depends on consistency across the entire analytical workflow—from biological sample preparation to mass spectrometry data acquisition and downstream analysis. Every step introduces potential sources of variability, and minimizing this analytical noise while preserving biological signal is essential.Automation emerged as a central theme of the workshop. Beyond improving throughput, automation fundamentally enhances reproducibility. Robotic sample preparation reduces variability associated with digestion, pipetting, and handling—enabling consistent performance across hundreds or thousands of samples, a scale that is difficult to achieve manually.<h3>Technological innovation powering a paradigm shift</h3>Recent advances in mass spectrometry are enabling researchers to bridge the gap between discovery and validation more efficiently than ever before. The workshop highlighted several Thermo Fisher Scientific platforms driving this shift:<ul><li>Thermo Scientific Orbitrap Astral mass spectrometer Optimized for discovery workflows, the Astral system delivers rapid acquisition and deep proteome coverage while maintaining high precision across large cohorts.</li><li>Thermo Scientific Stellar platform Designed for high-throughput targeted quantification, the Stellar platform supports scalable PRM workflows. Features such as PRM Conductor enable adaptive retention time tracking, which is essential for robust performance in large studies.</li><li>Thermo Scientific OptaSpray Ion Source (under evaluation) A hands-free ionization source designed to deliver consistent spray performance with simplified maintenance, supporting reproducibility across thousands of injections.</li></ul>Together, these technologies create a smoother continuum across assay tiers, shortening timelines and reducing barriers to clinical translation.<h3>Case study: Inflammatory bowel disease (IBD) cohort</h3>A key highlight of the presentation was a large-scale biomarker study in inflammatory bowel disease (IBD). The team conducted a 500-sample discovery analysis, followed by validation in a 1,000-sample cohort. Discovery was performed using the Orbitrap Astral system, while targeted validation employed the Stellar platform.This integrated approach successfully confirmed established biomarkers such as C-reactive protein (CRP) while also identifying novel protein candidates with strong translational potential. Importantly, these markers demonstrated consistency across discovery and validation data sets—underscoring the power of advanced instrumentation combined with robust workflows and informatics.<h3>From high quantity to high quality</h3>Historically, biomarker discovery has involved a tradeoff between throughput and data quality. The workshop demonstrated that this compromise is no longer necessary. By integrating automated sample preparation, optimized informatics, and next-generation mass spectrometry, researchers can now generate high-quality, high-throughput data that is both scalable and reproducible.This shift enables faster discovery while increasing confidence that candidate biomarkers are ready for downstream validation and clinical follow-up.<h3>The future of biomarker translation</h3>The workshop concluded on an optimistic note. Innovations in proteomics are fundamentally transforming biomarker development—shortening timelines, reducing costs, and accelerating the transition from discovery to clinical application.By integrating platforms such as Orbitrap Astral, Stellar, and OptaSpray, Thermo Fisher Scientific is helping advance a new era of translational biomarker science—where precision, scalability, and reliability work together to deliver meaningful clinical impact.Watch the full workshop recording to <a target="_blank" rel="noopener noreferrer" href="https://www.youtube.com/watch?v=ziyffQzZTwk">explore the data, workflows, and results behind this end-to-end approach to biomarker discovery and validation.</a><h3>Intended use disclaimer</h3>General Laboratory Equipment – Not For Diagnostic Procedures. Thermo Scientific general laboratory equipment, consumables, and software are intended for research, academic, and industrial use only. These products are not designed, validated, or approved for in vitro diagnostic or clinical use. Use of these products for diagnostic purposes may result in inaccurate results and potential health and safety risks.<h3>Resources</h3>Workshop &amp; educational content<ul><li>MSACL 2025 workshop recording: <a target="_blank" rel="noopener noreferrer" href="https://www.youtube.com/watch?v=ziyffQzZTwk">Accelerating Biomarker Discovery and Validation</a></li><li>Other MSACL workshop recordings coming soon</li></ul>Thermo Fisher Scientific Product Pages<ul><li><a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/products/2207">Thermo Scientific TSQ Certis Mass Spectrometer</a></li><li><a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/products/836">Thermo Scientific Orbitrap Astral Mass Spectrometer</a></li><li><a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/products/1149">Thermo Scientific Stellar Mass Spectrometer</a></li><li><a target="_blank" rel="noopener noreferrer" href="https://www.thermofisher.com/order/catalog/product/B51004132">Thermo Scientific OptaSpray Ion Source</a></li><li><a target="_blank" rel="noopener noreferrer" href="https://www.thermofisher.com/content/lifetech/global/en/home/industrial/mass-spectrometry/mass-spectrometer-price-affordability-programs.html">Mass spectrometer affordability programs</a> (Refurbished MS, trade-in, financing/ leasing)</li></ul>Peer-Reviewed Literature<ul><li>Baker ES et al. <a target="_blank" rel="noopener noreferrer" href="https://europepmc.org/article/MED/22943415">Mass spectrometry for translational proteomics: progress and clinical implications.</a> Genome Medicine (2012).</li><li>Fu Q et al. <a target="_blank" rel="noopener noreferrer" href="https://academic.oup.com/clinchem/article/62/1/198/5611803">An Empirical Approach to Signature Peptide Choice for Selected Reaction Monitoring.</a> Clinical Chemistry (2016).</li><li>Fu Q et al. <a target="_blank" rel="noopener noreferrer" href="https://doi.org/10.1002/mas.21750">Automated proteomic sample preparation: The key component for high throughput and quantitative mass spectrometry analysis.</a> Mass Spectrometry Reviews (2023).</li><li>Fu Q et al. <a target="_blank" rel="noopener noreferrer" href="https://doi.org/10.1021/acs.jproteome.7b00623">Highly Reproducible Automated Proteomics Sample Preparation Workflow for Quantitative Mass Spectrometry.</a> Journal of Proteome Research (2018).</li><li>Fu Q et al. <a target="_blank" rel="noopener noreferrer" href="https://doi.org/10.1002/anie.202409446">A Proteomics Pipeline for Generating Clinical Grade Biomarker Candidates from DIA-MS Discovery.</a> Angewandte Chemie International Edition (2024).</li><li>Fu Q et al. <a target="_blank" rel="noopener noreferrer" href="https://doi.org/10.1002/anie.202507610">Development and Clinical Evaluation of a Multiplexed Health Surveillance Panel Using Ultra High-Throughput PRM-MS in an Inflammatory Bowel Disease Cohort.</a> Angewandte Chemie International Edition (2025).</li></ul><blockquote>Visit us on&nbsp;<a target="_blank" rel="noopener noreferrer" href="https://www.linkedin.com/showcase/72003132/admin/dashboard/">LinkedIn:</a>&nbsp;#Omics #BiomarkerDiscovery #MassSpectrometry #ClinicalResearch</blockquote>

Accelerating Biomarker Discovery and Validation: From Discovery to Clinical Utility

Choosing the correct membrane for a syringe filter is an important step in achieving dependable and reproducible analytical results. Whether you work with nylon, PTFE, hydrophilic PTFE, or cellulose acetate filters, the membrane material directly influences filtration efficiency, sample integrity, and overall method performance. In this article, we explore why membrane selection matters, review the characteristics of common membrane materials, and provide practical guidance for choosing the best option for your application.<h4>Why Are Syringe Filters Important?</h4>Syringe filters are routinely used to remove particulate matter from samples prior to analysis. Unfiltered samples can introduce contaminants into chromatographic systems, leading to clogged columns, increased system backpressure, reduced instrument lifetime, and compromised analytical performance.Selecting an appropriate membrane is therefore essential for developing a robust and reliable HPLC workflow. An ideal membrane effectively removes particles while preserving the original composition of the sample. Since the membrane comes into direct contact with both the analyte and the sample solvent, compatibility must always be carefully considered.<img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/KNAUER_syringe_filters_f3eaa1381e.webp" alt="KNAUER: Syringe filters" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_KNAUER_syringe_filters_f3eaa1381e.webp 156w," sizes="100vw" width="720" height="720">Let’s examine the key factors involved in choosing the most suitable membrane.<h4>Step 1: Consider the Nature of Your Sample</h4>The first and most important factor is the chemical composition of the sample and the solvents involved. Different membrane materials offer different chemical resistance and sample interaction characteristics.<h5>Nylon Membranes</h5>Nylon is one of the most commonly used membrane materials in HPLC laboratories because of its broad compatibility with aqueous solutions and many organic solvents.Recommended applications:<ul><li>Routine filtration of HPLC samples containing aqueous solvents, organic solvents, or mixed mobile phases.</li><li>Removal of suspended particles prior to chromatographic injection.</li><li>General-purpose laboratory filtration where versatility is required.</li></ul>Avoid using Nylon when:<ul><li>Working with strongly acidic solutions or samples with pH values below 3.</li><li>Filtering protein-containing samples, since proteins can adsorb to the membrane surface and reduce recovery.</li></ul><h5>Cellulose Acetate (CA) Membranes</h5>Cellulose acetate membranes exhibit very low protein binding and are widely used for biological and biochemical applications.Recommended applications:<ul><li>Filtration of biological fluids such as serum, plasma, and cell culture media.</li><li>Applications where protein recovery must be maximized.</li><li>Aqueous laboratory procedures involving enzymes or other sensitive biomolecules.</li></ul>Avoid using CA when:<ul><li>Filtering samples containing organic solvents that may damage the membrane.</li><li>Working under highly acidic or highly alkaline conditions that could degrade the material.</li></ul><h5>Hydrophilic PTFE (hPTFE) Membranes</h5>Hydrophilic PTFE combines the exceptional chemical resistance of PTFE with the ability to process aqueous samples without pre-wetting.Recommended applications:<ul><li>Filtration of both aqueous and organic solutions.</li><li>HPLC sample preparation involving mixed solvent systems.</li><li>Applications requiring compatibility across a broad pH range.</li></ul>Avoid using hPTFE when:<ul><li>Processing highly viscous samples that may rapidly clog the membrane.</li><li>Performing PFAS analysis, where fluorinated materials may introduce unwanted contamination.</li></ul><h5>PTFE Membranes</h5>PTFE membranes provide excellent resistance to aggressive chemicals and are especially suitable for demanding solvent environments.Recommended applications:<ul><li>Samples containing strong acids, strong bases, or aggressive organic solvents.</li><li>Environmental, industrial, and chemical analyses involving non-polar solvents such as hexane, toluene, or acetone.</li><li>Filtration of predominantly organic solvent-based samples.</li></ul>Avoid using PTFE when:<ul><li>Filtering aqueous solutions without first wetting the membrane or using a hydrophilic PTFE alternative.</li><li>Working with protein-rich or highly hydrophilic samples where adsorption or poor wetting may occur.</li></ul><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/KNAUER_Fig_1_4_Nylon_top_left_CA_top_right_h_PTFE_bottom_left_and_PTFE_bottom_right_86a6481d0b.jpg" alt="KNAUER: Fig. 1–4 Nylon (top left), CA (top, right), hPTFE (bottom, left), and PTFE (bottom, right)" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_KNAUER_Fig_1_4_Nylon_top_left_CA_top_right_h_PTFE_bottom_left_and_PTFE_bottom_right_86a6481d0b.jpg 157w," sizes="100vw" width="1111" height="1107"><h4>Step 2: Select an Appropriate Pore Size</h4>The membrane pore size determines the level of particulate removal and should be selected according to the requirements of the analytical method.Common pore sizes include:<ul><li>0.2 µm – Suitable for removing very fine particles and for sterile filtration. Frequently used in UHPLC and LC-MS workflows.</li><li>0.45 µm – The standard choice for most routine HPLC applications.</li><li>1.0 µm and larger – Often used for coarse filtration or as a pre-filtration step for heavily contaminated samples.</li></ul>Choosing the correct pore size helps protect instrumentation while minimizing unnecessary sample loss.<img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/KNAUER_Fig_1_2_0_22_m_left_and_0_45_m_right_Nylon_Syringe_Filter_48ffc3d777.jpg" alt="KNAUER: Fig. 1–2 0.22 µm (left), and 0.45 µm (right) Nylon Syringe Filter" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_KNAUER_Fig_1_2_0_22_m_left_and_0_45_m_right_Nylon_Syringe_Filter_48ffc3d777.jpg 245w," sizes="100vw" width="1177" height="573"><h4>Step 3: Verify Chemical Compatibility</h4>Before selecting a membrane, confirm that it is compatible with both the sample matrix and the solvents present. Incompatible materials may swell, degrade, release extractables, or alter the composition of the sample.Consulting a solvent compatibility chart is highly recommended whenever unfamiliar solvents or mixtures are involved.<h4>Step 4: Match the Membrane to the Application</h4>Different analytical applications often benefit from specific membrane types.<h5>HPLC Analysis</h5><ul><li>Nylon and PTFE membranes are among the most frequently used options due to their broad chemical compatibility and excellent filtration performance.</li></ul><h5>Protein and Biomolecule Analysis</h5><ul><li>CA, PVDF, and PES membranes are generally preferred because they exhibit low protein binding and help preserve sample recovery.</li></ul><h5>Microbiological Testing</h5><ul><li>Mixed cellulose ester (MCE) membranes are commonly employed for microbial monitoring and microbiological quality control procedures.</li></ul>Selecting a membrane tailored to the analytical objective can significantly improve data quality and method reliability.<h4>Step 5: Evaluate Product Quality and Certification</h4>Not all syringe filters are manufactured to the same standards. For critical applications, select products that comply with recognized quality systems and certifications such as ISO standards or CE requirements.This becomes particularly important in regulated industries, where traceability, consistency, and documented performance are essential.<h4>Practical Recommendations for Using Syringe Filters</h4>Following good filtration practices can further improve analytical performance.<ul><li>Pre-Wet Hydrophobic Membranes: Hydrophobic materials such as PTFE often require pre-wetting with a compatible organic solvent before filtering aqueous samples.</li><li>Avoid Excessive Pressure: Applying excessive force may rupture the membrane or cause leakage. Luer-lock syringes are recommended to provide secure connections and controlled pressure.</li><li>Use Filters Only Once: Syringe filters are intended for single-use operation. Reusing filters increases the risk of carryover and cross-contamination.</li><li>Dispose of Filters Properly: Used syringe filters should be discarded according to laboratory safety procedures and local waste disposal regulations.</li></ul><h4>Final Thoughts</h4>Selecting the right syringe filter membrane is a key factor in obtaining accurate, reproducible, and reliable analytical results. By considering sample composition, solvent compatibility, pore size, and application requirements, laboratories can choose filtration solutions that protect instruments, preserve sample integrity, and improve overall analytical performance.A carefully selected membrane may seem like a small detail, but it can have a substantial impact on the quality and robustness of your entire analytical workflow.<blockquote>If you want to optimize your HPLC workflow, check out our brand-new <a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/products/2380">SEPAPURE Syringe Filter</a>.</blockquote>

How to Choose the Right Membrane for Your Syringe Filter

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Based in Berlin, KNAUER is a medium-sized, owner-managed company that has been serving the sciences since 1962. We develop and manufacture scientific instruments of superior quality for liquid chromatography, including: Analytical HPLC/UHPLC, Preparative HPLC, Fast protein liquid chromatography (FPLC), Multi-column chromatography/Simulated moving bed (SMB) chromatography. High pressure dosing, in-line detection systems, and osmometry are additional business areas.

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Phenomenex Strata C18-E Solid Phase Extraction (SPE)

Strata C18-E is a silica-based end-capped C18 solid phase extraction (SPE) sorbent.

Strong Hydrophobic Retention Solid Phase Extraction (SPE)Strata C18-E is a silica-based end-capped C18 solid phase extraction (SPE) sorbent that offers strong hydrophobic retention with negligible secondary polar interactions from active silanol groups. Strata C18-E can be best used for the extraction of hydrophobic or polar organic analytes from aqueous matrices. In addition, Strata SPE sorbents are manufactured with the highest purity and extensively tested to give clean extracts.Strata C18-E is compatible with various formats, including C18 SPE cartridges. Our C18 SPE products are synonymous with quality, offering the highest purity and undergo rigorous testing to deliver consistently clean extracts for your analytical needs.<img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Phenomenex_Strata_C18_E_748c192b52.webp" alt="Phenomenex: Strata-C18-E" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Phenomenex_Strata_C18_E_748c192b52.webp 245w," sizes="100vw" width="500" height="150"><ul><li>Surface Area (m²/g): 500, m²/g</li><li>Separation Mode: Reversed Phase</li><li>Pore Size: 70, 100</li><li>Particle Size: 20, 55</li><li>Recommended Use: Extraction of hydrophobic molecules</li></ul><h4>Starting Method</h4><h5>Strata Reversed Phase</h5><ul><li>Condition: 1 mL Methanol</li><li>Equilibrate: 1 mL DI Water</li><li>Load: Pretreated sample</li><li>Wash: 1 mL 5% Methanol in DI Water, dry under vacuum for 2-5 min</li><li>Elute: 1 mL Methanol</li></ul>Based on 100 mg/1 mL sorbent mass. The above is a convenient starting point for SPE method development, further optimization may be required to tailor the method for your specific extraction needs.

Phenomenex β-Gone β-Glucuronidase Removal 96-well plates and tubes

β-Gone β-Glucuronidase Removal 96-well plates and tubes are designed to target and remove β-glucuronidase from hydrolyzed urine samples without requiring additional time or method development.

β-Gone™ offers a simple, fast, and highly targeted method to remove β-Glucuronidase from urine samples prior to analysis. Within 1 minute, with no necessary method development, your samples will be ready for analysis.<h4>Rapid Cleanup of Hydrolyzed Urine</h4><ul><li>Increase HPLC/UHPLC Column Lifetime</li><li>Reduce Mass Spec Maintenance</li><li>Maintain the Selectivity of Your HPLC/UHPLC Columm</li></ul><h3>Available Formats &amp; Protocols:</h3><h5>β-Gone Plus 96-Well Plates Protocol:</h5><ul><li>Follow β-glucuronidase vendor specifications and add appropriate amount of enzyme and urine to the well*&nbsp;<ul><li>Please note: Do not use more than 10% organic solution for the incubation and ensure that &nbsp;organic solution is added last.</li></ul></li><li>Add 133 µL of 0.1% Formic acid in Methanol</li><li>Initiate vacuum or positive pressure (2-5 psi) and collect eluent to inject**</li></ul><h5>β-Gone 96-Well Plates and 1 mL Tubes Protocol:</h5><ul><li>Dilute 200 µL of urine hydrolysate with 133 µL of 0.1% Formic acid in Methanol</li><li>Load diluted sample onto plate/tube and apply vacuum &nbsp;or positive pressure (2-5 psi) and collect eluent</li><li>Inject eluent**</li></ul><h5>2 mL Centrifuge Tubes Protocol:</h5><ul><li>Tap tube prior to use to consolidate sorbent&nbsp;</li><li>Combine 200 µL of Urine Hydrolysate with 133 µL of 0.1 % Formic acid in Methanol&nbsp;</li><li>Load into the centrifuge tube, cap, and invert 10x. Vortex for 30 seconds.</li><li>Centrifuge tube at 14000 rpm for 10 min (16000 xg RCF)&nbsp;</li><li>Collect supernatant to inject for analysis** &nbsp;</li></ul>*For urine samples &lt; 200 µL, use 30 mg β-Gone Plus Plate&nbsp; For urine samples &nbsp;≥ 200 µL, use 60 mg β-Gone Plus Plate&nbsp; **Depending on injection volume, starting %B (organic) in mobile phase, and column dimensions, a dilution of 1:1 with aqueous mobile &nbsp;phase may be required to prevent peak distortion of early eluting compounds.&nbsp;<h4>Ordering Information</h4>β-Gone β-Glucuronidase Removal Products<ul><li>8B-S139-TAK: 1 mL Tubes, Recombinant Enzyme, 100/Box</li><li>8B-S322-DAK: 1 mL Tubes, Non-Recombinant Enzyme, 100/Box</li><li>8E-S139-TGA: 96-Well Plate, Recombinant Enzyme, 1/Box&nbsp;</li><li>8E-S322-DGA: 96-Well Plate, Non-Recombinant Enzyme, 1/Box&nbsp;</li><li>8N-S323-TUK: 2 mL Centrifuge Tubes, Recombinant and Non-Recombinant Enzyme, 100/Box&nbsp;</li><li>8E-S323-TGA: 96-Well Plate Plus 30 mg/well, Recombinant/Non-Recombinant Enzyme, 1/Box</li><li>8B-S323-UGA: 96-Well Plate Plus 60 mg/well, Recombinant/Non-Recombinant Enzyme, 1/Box &nbsp;</li></ul>

Phenomenex Kinetex Core-Shell Polar C18 LC Columns

The Kinetex® 2.6 µm Polar C18 is a core-shell based particle with a polar modified surface and a C18 alkyl phase.

The Kinetex® 2.6 µm Polar C18 is a core-shell based particle with a polar modified surface and a C18 alkyl phase. This versatile column can easily upgrade existing fully porous 5 µm and 3 µm methods to shorten run time, increase sensitivity, and even provide greater resolution with much higher efficiency levels. Combined with being 100 % aqueous stable, the Kinetex Polar C18 is a unique column that combines performance and selectivity.<ul><li>Compatible with All Analytical HPLC/UHPLC Systems</li><li>L:1 C18 with Unique Polar Retention</li><li>Compatible with 100 % Aqueous Mobile Phases</li><li>USP: L1</li></ul><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Phenomenex_Kinetex_polar_C18_60ab4fb700.webp" alt="Phenomenex: Kinetex-polar-C18" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Phenomenex_Kinetex_polar_C18_60ab4fb700.webp 245w," sizes="100vw" width="500" height="150"><ul><li>Carbon Load: 9 (effective), 9 (effective) %</li><li>Particle Size (μm): 2.6, 3</li><li>Recommended Use: Combined C18 and polar modified surface that provides polar and non-polar retention alongside 100% aqueous stability</li></ul><h4>C18 and Polar Selectivity</h4>Combined C18 and polar modified surface that provides polar and non-polar retention alongside 100 % aqueous stability.<h4>Increase Polar Retention and Hydrophobic Selectivity</h4>Increase polar selectivity without sacrificing hydrophobic selectivity with the Kinetex Polar C18. The Kinetex Polar C18 is an excellent all-purpose, 100% aqueous stable phase for use with multi-compound mixes that contain polar and non-polar compounds, or even single class methods that have closely related compounds, impurities, or metabolites.<h4>Kinetex Where Performance Meets the Right Selectivity</h4>Quickly improve the productivity and performance of your existing methods and utilize the core-shell advantage to deliver performance you need at backpressures the system can handle. Select from a variety of column selectivity to find the right phase for the application.<h3>Kinetex Core-Shell Technology Columns</h3>Kinetex Core-Shell Technology delivers dramatic improvements in efficiency over conventional fully porous media, which can be leveraged to increase resolution, significantly improve productivity, reduce solvent consumption, and decrease costs. Whether you are running high-performance liquid chromatography (HPLC) or ultra-high performance liquid chromatography (UHPLC) methods, the Kinetex core-shell family can deliver shockingly improved performance over the current column you are using. Phenomenex designs, manufactures, and sells its very own silica and organo-silica core-shell particles.Advantages of Kinetex HPLC Columns<ul><li>Performance gains on ANY liquid chromatography system</li><li>System-to-system and lab-to-lab method portability</li><li>Improve the productivity of older, established methods.</li></ul><h4>How Does Core-Shell Technology Work?</h4>Core-shell technology in chromatography is a revolutionary approach that enhances the efficiency of HPLC systems while minimizing costs. Core-shell particles are designed with a solid, non-porous silica core surrounded by a porous outer layer. This unique structure allows for faster analyte mass transfer between the mobile and stationary phases, as diffusion occurs only through the thin, porous shell rather than the entire particle. This minimizes band broadening, leading to higher separation efficiency and sharper peaks.The consistency in size and shape of core-shell particles further enhances separation efficiency by reducing variability in analyte movement. As a result, core-shell HPLC columns provide high efficiency and resolution at lower backpressures, making them an ideal choice for labs looking to improve their existing HPLC systems without the need for costly UHPLC equipment.Core-shell technology thus offers a cost-effective alternative to upgrading to UHPLC systems, enabling labs to maximize the efficiency of their current HPLC setups. The formation of these particles through advanced sol-gel processing techniques ensures uniformity and reliability, making them a valuable tool in modern chromatography. By using the Kinetex HPLC column, laboratories can achieve faster analysis times and higher productivity while maintaining excellent separation performance.<h3>Kinetex Core-Shell Performance on Any LC System</h3>The 5 versatile particle sizes allow you to select the best fit for your instrument.<img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Phenomenex_HPLC_and_UHPLC_Kinetex_Core_Shell_Technology_Columns_5_particle_sizes_bbed75c36f.jpg" alt="Phenomenex: Five versatile particle sizes of Kinetex Core-Shell Technology Columns" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Phenomenex_HPLC_and_UHPLC_Kinetex_Core_Shell_Technology_Columns_5_particle_sizes_bbed75c36f.jpg 245w," sizes="100vw" width="1341" height="333"><h3>Particle Chemistry</h3>Using sol-gel processing techniques that incorporate nano-structuring technology, a durable, homogeneous porous shell is grown on a solid silica core to create a Kinetex Core-Shell particle. This particle morphology results in less band broadening for all four sources of UHPLC band broadening compared to fully porous particles and thus delivers extremely high efficiencies.<ul><li>Optimized for Ultra-High Performance</li><li>Superior Core-Shell Quality</li><li>Core-Shell Scalability and Portability</li></ul>Kinetex particles are characterized by an exceptionally consistent size distribution, with particles being nearly monodispersed, meaning they are uniform in size. This precise uniformity plays a crucial role in minimizing variability within the column, leading to enhanced separation efficiency and sharper peaks.The nearly monodispersed nature of these particles ensures that the flow of the mobile phase remains consistent, reducing the likelihood of irregularities that can impact results. As a result, Kinetex columns deliver highly reliable and reproducible performance, making them ideal for achieving consistent analytical outcomes acrLC Kinetex Core-Shell Technolooss various applications.<img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Phenomenex_HPLC_and_UHPLC_Kinetex_Core_Shell_Technology_Columns_Kinetex_Polar_C18_vs_Fully_Porous_phase_a2729b64e5.jpg" alt="Phenomenex: Kinetex Polar C18 vs Fully Porous phase" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Phenomenex_HPLC_and_UHPLC_Kinetex_Core_Shell_Technology_Columns_Kinetex_Polar_C18_vs_Fully_Porous_phase_a2729b64e5.jpg 245w," sizes="100vw" width="1376" height="581"><h3>Core-shell vs Fully Porous</h3><h4>High-Efficiency‚ High-Density Particle</h4>The combination of a consistent, solid, high-density core along with proprietary column packing technologies in the Kinetex HPLC column ensures optimum bed structure and high column performance. This carefully designed core structure promotes uniform packing of the particles, which is essential for minimizing band-broadening effects that can arise from Eddy Diffusion.The manufacturing process of Kinetex columns is tightly controlled, focusing on factors such as particle size distribution, surface and bonding homogeneity, and packing quality. This meticulous engineering leads to uniform packing and minimized band-broadening, resulting in superior separation efficiency.<img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Phenomenex_Core_shell_vs_Fully_Porous_60601b6fa3.jpg" alt="Phenomenex: Core-shell vs Fully Porous." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Phenomenex_Core_shell_vs_Fully_Porous_60601b6fa3.jpg 245w," sizes="100vw" width="904" height="506"><h3>Expand Your Core-Shell Selectivity Toolbox</h3>Kinetex core-shell particle LC columns deliver exceptional performance enhancements across any LC system, offering unmatched efficiency and resolution. With a choice of 11 diverse stationary phases, these columns cater to a wide range of chromatographic applications, ensuring optimal separation for various analytes.Additionally, the availability of five versatile particle sizes allows for precise method tailoring, enabling both high-speed and high-resolution separations. Whether you're using HPLC or UHPLC systems, Kinetex columns provide the flexibility and performance needed to achieve superior analytical results.<h3>Top 5 Pros of Kinetex Core-Shell Technology</h3><ul><li>Productivity: Kinetex Core-Shell Technology significantly enhances method productivity with minimal effort. By simply replacing traditional, fully porous columns with Kinetex HPLC columns, users can achieve better resolution, sensitivity, and overall productivity without extensive method redevelopment. This easy switch allows for immediate improvements in chromatographic performance, making it a cost-effective upgrade to existing methods.</li><li>Portability The Kinetex Core-Shell columns are available in a range of particle sizes (1.3 µm to 5 µm), which ensures method portability across different HPLC and UHPLC systems. This scalability allows for consistent retention and selectivity across various particle sizes while accommodating different backpressure levels, making it easier to transfer methods between instruments.</li><li>Performance Kinetex Core-Shell Technology delivers superior chromatographic efficiency compared to fully porous particles. This efficiency boost enables chromatographers to maximize the performance of their HPLC/UHPLC systems, ensuring optimal results with higher resolution and sharper peaks, even under similar method conditions.</li><li>Phases With 11 unique phase selectivities available, Kinetex Core-Shell Technology offers unmatched versatility for method development. This wide selection of phase chemistries allows for tailored separations and simplified optimization, ensuring the best possible results for a wide range of applications.</li><li>Pressure Kinetex Core-Shell columns provide higher efficiency at lower system backpressures compared to fully porous columns. This allows users to achieve superior chromatographic performance on conventional LC instruments without the need for ultra-high-pressure systems, making it a practical choice for routine analyses.</li></ul>

Phenomenex Kinetex XB-C18 Core Shell Columns

Di-isobutyl side chains differentiate this C18 column. Low ligand density and an inactive surface make this column a great hydrogen bond acceptor. 

<h4>A Unique Core-Shell Selectivity</h4>Di-isobutyl side chains differentiate this C18 column. Low ligand density and an inactive surface make this column a great hydrogen bond acceptor. This phase will demonstrate improved peak shape and increased retention of acidic compounds.<ul><li>Compatible with all Analytical HPLC/UHPLC Systems</li><li>Unique C18 Retention (USP: L1)</li><li>Improved Selectivity of Acidic compounds</li></ul><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Phenomenex_Kinetex_XB_C18_Core_Shell_HPLC_Column_411f3ff1a8.webp" alt="Phenomenex: Kinetex XB-C18 Core Shell HPLC Column" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Phenomenex_Kinetex_XB_C18_Core_Shell_HPLC_Column_411f3ff1a8.webp 245w," sizes="100vw" width="500" height="150"><ul><li>Carbon Load: 10 (effective), 10.0, null %, 10 (effective) %, 10.0 %</li><li>Surface Area (m²/g): 200</li><li>Particle Size (μm): 1.7, 2.6, 3.5, 5</li><li>Recommended Use: Superior peak shape and enhanced separation of basic compounds under neutral and acidic conditions</li></ul><h4>Selectivity for Acidic Compounds</h4>This unique C18 phase yields increased hydrogen bonding with hydrophobic selectivity‚ resulting in improved peak shape for basic compounds and increased retention of acidic compounds.<h4>Complementary Core-Shell C18 Phases</h4>Ultra-high performance columns like Kinetex will give you very narrow peaks, but without the right selectivities you are left with very narrow, overlapping peaks. The phases offered in the Kinetex column line are complementary to one another, so the spectrum of selectivity your separations require is covered.<img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Phenomenex_Kinetex_C18_vs_XB_C18_fe7c0252f4.jpg" alt="Phenomenex: Kinetex C18 vs. XB-C18" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Phenomenex_Kinetex_C18_vs_XB_C18_fe7c0252f4.jpg 165w," sizes="100vw" width="902" height="855"><h4>Manipulate Acidic and Basic Compound Retention</h4>Find the right phase selectivity for your application by investigating the diverse selectivity availability of the Kinetex Core Shell HPLC and UHPLC columns. Where reliable performance meets unique selectivity.<blockquote>Stationary phase Kinetex XB-C18 is provided in these column types: analytical and preparative</blockquote><h3>Kinetex Core-Shell Technology Columns</h3>Kinetex Core-Shell Technology delivers dramatic improvements in efficiency over conventional fully porous media, which can be leveraged to increase resolution, significantly improve productivity, reduce solvent consumption, and decrease costs. Whether you are running high-performance liquid chromatography (HPLC) or ultra-high performance liquid chromatography (UHPLC) methods, the Kinetex core-shell family can deliver shockingly improved performance over the current column you are using. Phenomenex designs, manufactures, and sells its very own silica and organo-silica core-shell particles.Advantages of Kinetex HPLC Columns<ul><li>Performance gains on ANY liquid chromatography system</li><li>System-to-system and lab-to-lab method portability</li><li>Improve the productivity of older, established methods.</li></ul><h4>How Does Core-Shell Technology Work?</h4>Core-shell technology in chromatography is a revolutionary approach that enhances the efficiency of HPLC systems while minimizing costs. Core-shell particles are designed with a solid, non-porous silica core surrounded by a porous outer layer. This unique structure allows for faster analyte mass transfer between the mobile and stationary phases, as diffusion occurs only through the thin, porous shell rather than the entire particle. This minimizes band broadening, leading to higher separation efficiency and sharper peaks.The consistency in size and shape of core-shell particles further enhances separation efficiency by reducing variability in analyte movement. As a result, core-shell HPLC columns provide high efficiency and resolution at lower backpressures, making them an ideal choice for labs looking to improve their existing HPLC systems without the need for costly UHPLC equipment.Core-shell technology thus offers a cost-effective alternative to upgrading to UHPLC systems, enabling labs to maximize the efficiency of their current HPLC setups. The formation of these particles through advanced sol-gel processing techniques ensures uniformity and reliability, making them a valuable tool in modern chromatography. By using the Kinetex HPLC column, laboratories can achieve faster analysis times and higher productivity while maintaining excellent separation performance.<h3>Kinetex Core-Shell Performance on Any LC System</h3>The 5 versatile particle sizes allow you to select the best fit for your instrument.<img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Phenomenex_HPLC_and_UHPLC_Kinetex_Core_Shell_Technology_Columns_5_particle_sizes_bbed75c36f.jpg" alt="Phenomenex: Five versatile particle sizes of Kinetex Core-Shell Technology Columns" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Phenomenex_HPLC_and_UHPLC_Kinetex_Core_Shell_Technology_Columns_5_particle_sizes_bbed75c36f.jpg 245w," sizes="100vw" width="1341" height="333"><h3>Particle Chemistry</h3>Using sol-gel processing techniques that incorporate nano-structuring technology, a durable, homogeneous porous shell is grown on a solid silica core to create a Kinetex Core-Shell particle. This particle morphology results in less band broadening for all four sources of UHPLC band broadening compared to fully porous particles and thus delivers extremely high efficiencies.<ul><li>Optimized for Ultra-High Performance</li><li>Superior Core-Shell Quality</li><li>Core-Shell Scalability and Portability</li></ul>Kinetex particles are characterized by an exceptionally consistent size distribution, with particles being nearly monodispersed, meaning they are uniform in size. This precise uniformity plays a crucial role in minimizing variability within the column, leading to enhanced separation efficiency and sharper peaks.The nearly monodispersed nature of these particles ensures that the flow of the mobile phase remains consistent, reducing the likelihood of irregularities that can impact results. As a result, Kinetex columns deliver highly reliable and reproducible performance, making them ideal for achieving consistent analytical outcomes acrLC Kinetex Core-Shell Technolooss various applications.<img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Phenomenex_HPLC_and_UHPLC_Kinetex_Core_Shell_Technology_Columns_Kinetex_Polar_C18_vs_Fully_Porous_phase_a2729b64e5.jpg" alt="Phenomenex: Kinetex Polar C18 vs Fully Porous phase" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Phenomenex_HPLC_and_UHPLC_Kinetex_Core_Shell_Technology_Columns_Kinetex_Polar_C18_vs_Fully_Porous_phase_a2729b64e5.jpg 245w," sizes="100vw" width="1376" height="581"><h3>Core-shell vs Fully Porous</h3><h4>High-Efficiency‚ High-Density Particle</h4>The combination of a consistent, solid, high-density core along with proprietary column packing technologies in the Kinetex HPLC column ensures optimum bed structure and high column performance. This carefully designed core structure promotes uniform packing of the particles, which is essential for minimizing band-broadening effects that can arise from Eddy Diffusion.The manufacturing process of Kinetex columns is tightly controlled, focusing on factors such as particle size distribution, surface and bonding homogeneity, and packing quality. This meticulous engineering leads to uniform packing and minimized band-broadening, resulting in superior separation efficiency.<img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Phenomenex_Core_shell_vs_Fully_Porous_60601b6fa3.jpg" alt="Phenomenex: Core-shell vs Fully Porous.jpg" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Phenomenex_Core_shell_vs_Fully_Porous_60601b6fa3.jpg 245w," sizes="100vw" width="904" height="506"><h3>Expand Your Core-Shell Selectivity Toolbox</h3>Kinetex core-shell particle LC columns deliver exceptional performance enhancements across any LC system, offering unmatched efficiency and resolution. With a choice of 11 diverse stationary phases, these columns cater to a wide range of chromatographic applications, ensuring optimal separation for various analytes.Additionally, the availability of five versatile particle sizes allows for precise method tailoring, enabling both high-speed and high-resolution separations. Whether you're using HPLC or UHPLC systems, Kinetex columns provide the flexibility and performance needed to achieve superior analytical results.<h3>Top 5 Pros of Kinetex Core-Shell Technology</h3><ul><li>Productivity: Kinetex Core-Shell Technology significantly enhances method productivity with minimal effort. By simply replacing traditional, fully porous columns with Kinetex HPLC columns, users can achieve better resolution, sensitivity, and overall productivity without extensive method redevelopment. This easy switch allows for immediate improvements in chromatographic performance, making it a cost-effective upgrade to existing methods.</li><li>Portability The Kinetex Core-Shell columns are available in a range of particle sizes (1.3 µm to 5 µm), which ensures method portability across different HPLC and UHPLC systems. This scalability allows for consistent retention and selectivity across various particle sizes while accommodating different backpressure levels, making it easier to transfer methods between instruments.</li><li>Performance Kinetex Core-Shell Technology delivers superior chromatographic efficiency compared to fully porous particles. This efficiency boost enables chromatographers to maximize the performance of their HPLC/UHPLC systems, ensuring optimal results with higher resolution and sharper peaks, even under similar method conditions.</li><li>Phases With 11 unique phase selectivities available, Kinetex Core-Shell Technology offers unmatched versatility for method development. This wide selection of phase chemistries allows for tailored separations and simplified optimization, ensuring the best possible results for a wide range of applications.</li><li>Pressure Kinetex Core-Shell columns provide higher efficiency at lower system backpressures compared to fully porous columns. This allows users to achieve superior chromatographic performance on conventional LC instruments without the need for ultra-high-pressure systems, making it a practical choice for routine analyses.</li></ul>

Phenomenex Strata SCX Solid Phase Extraction (SPE)

A benzene sulfonic acid group is bonded to the surface of the silica particle, giving strong cation-exchange selectivity.

Ion-Exchange Solid Phase Extraction (SPE) SorbentA benzene sulfonic acid group is bonded to the surface of the silica particle, giving strong cation-exchange selectivity.<ul><li>Extraction of 1 °, 2 °, and 3 amines</li></ul><figure class="image"><img style="aspect-ratio:791/384;" src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Phenomenex_Strata_SCX_SPE_f35df1035c.jpg" alt="Phenomenex: Strata SCX" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Phenomenex_Strata_SCX_SPE_f35df1035c.jpg 245w," sizes="100vw" width="791" height="384"></figure><ul><li>Surface Area (m²/g): -</li><li>Separation Mode: Ion Exchange</li><li>Particle Size: 55</li><li>Recommended Use: Extraction of weakly basic compounds</li></ul><h4>Starting Method</h4><h5>Strata SCX – Strong Cation-Exchange</h5><ul><li>Condition: 1 mL Methanol</li><li>Equilibrate: 1 mL Acidified Water</li><li>Load: Pretreated sample (acidified)</li><li>Wash 1: 1 mL 0.1N HCl in Water</li><li>Wash 2: 1 mL 0.1N HCl in Methanol, dry under vacuum for 2-5 min</li><li>Elute: 1 mL 5 % NH4OH in Methanol</li></ul>Based on 100 mg/1 mL sorbent mass. The above is a convenient starting point for SPE method development, further optimization may be required to tailor the method for your specific extraction needs.

Phenomenex Strata NH2 Solid Phase Extraction (SPE)

This amino phase offers strong polar selectivity and hydrogen bonding under normal phase conditions or can be used as a weak anion-exchange sorbent.

Normal Phase Solid Phase Extraction (SPE)This amino phase offers strong polar selectivity and hydrogen bonding under normal phase conditions or can be used as a weak anion-exchange sorbent.<figure class="image"><img style="aspect-ratio:500/150;" src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Phenomenex_Strata_nh2_a7c3599f55.webp" alt="Phenomenex: Strata-NH2" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Phenomenex_Strata_nh2_a7c3599f55.webp 245w," sizes="100vw" width="500" height="150"></figure><ul><li>Surface Area (m²/g): -</li><li>Separation Mode: Normal Phase</li><li>Pore Size: 100</li><li>Particle Size: 25, 55</li><li>Recommended Use: Strong polar selectivity and hydrogen bonding under normal phase conditions or can be used as a weak anion-exchange sorbent</li></ul><h4>Starting Method</h4><h5>Strata NH2 – Weak Anion-Exchange</h5><ul><li>Condition: 1 mL Methanol</li><li>Equilibrate: 1 mL Water, pH 6-7</li><li>Load: Pretreated sample, pH 6-7</li><li>Wash 1: 1 mL 25mM Ammonium Acetate Buffer, pH 6-7</li><li>Wash 2: 1 mL Methanol, dry under vacuum for 2-5 min</li><li>Elute Any Acid: 1 mL 5% NH4OH in Methanol</li><li>Elute Weak Acids: 1 mL 5% Formic Acid in Methanol</li></ul>Based on 100 mg/1 mL sorbent mass. The above is a convenient starting point for SPE method development, further optimization may be required to tailor the method for your specific extraction needs.

Phenomenex Strata SAX Solid Phase Extraction (SPE)

The quaternary amine group on this sorbent remains positively charged under all conditions, giving a strong anion-exchange mechanism of retention.

Ion-Exchange Solid Phase Extraction Sorbent (SPE)The quaternary amine group on this sorbent remains positively charged under all conditions, giving a strong anion-exchange mechanism of retention.<figure class="image"><img style="aspect-ratio:500/150;" src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Phenomenex_Strata_sax_470d923b27.webp" alt="Phenomenex: Strata-SAX" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Phenomenex_Strata_sax_470d923b27.webp 245w," sizes="100vw" width="500" height="150"></figure><ul><li>Surface Area (m²/g): -</li><li>Separation Mode: Ion Exchange</li><li>Particle Size: 55</li><li>Recommended Use: Extraction of weakly acidic compounds</li></ul><h4>Starting Method</h4><h5>Strata SAX – Strong Anion-Exchange</h5><ul><li>Condition: 1 mL Methanol</li><li>Equilibrate: 1 mL DI Water</li><li>Load: Pretreated sample, pH 6-7</li><li>Wash 1: 1 mL 25mM Ammonium Acetate Buffer, pH 6-7</li><li>Wash 2: 1 mL Methanol, dry under vacuum for 2-5 min</li><li>Elute: 1 mL 5% Formic Acid in Methanol</li></ul>Based on 100 mg/1 mL sorbent mass. The above is a convenient starting point for SPE method development, further optimization may be required to tailor the method for your specific extraction needs.

Phenomenex Strata Screen-A Solid Phase Extraction (SPE)

Incorporates the hydrophobic selectivity of a C8 phase and strong anion-exchange for the extraction of acidic drugs from biological matrices.

Mixed-Mode Solid Phase Extraction (SPE) SorbentIncorporates the hydrophobic selectivity of a C8 phase and strong anion-exchange for the extraction of acidic drugs from biological matrices.<figure class="image"><img style="aspect-ratio:500/150;" src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Phenomenex_Strata_Screen_A_f370bdfd48.webp" alt="Phenomenex: Strata Screen-A" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Phenomenex_Strata_Screen_A_f370bdfd48.webp 245w," sizes="100vw" width="500" height="150"></figure><ul><li>Surface Area (m²/g): -</li><li>Separation Mode: Multi-Mode</li><li>Particle Size: 55</li><li>Recommended Use: Extracts acidic drugs from biological matrices</li></ul><h4>Starting Method</h4><h5>Strata Screen-A Strong Anion-Exchange</h5><ul><li>Condition: 1 mL Methanol</li><li>Equilibrate: 1 mL Water</li><li>Load: Pretreated sample</li><li>Wash 1: 1 mL 25mM Ammonium Acetate Buffer, pH 6-7</li><li>Wash 2: 1 mL Methanol, dry under vacuum for 2-5 min</li><li>Elute: 1 mL 5% Formic Acid in Methanol</li></ul>Based on 100 mg/1 mL sorbent mass. The above is a convenient starting point for SPE method development, further optimization may be required to tailor the method for your specific extraction needs.

Phenomenex Strata Screen-C Solid Phase Extraction (SPE)

Incorporates the hydrophobic selectivity of a C8 phase and strong cation exchange for the extraction of basic drugs from biological matrices.

Strong Cation-Exchange Solid Phase Extraction (SPE)Incorporates the hydrophobic selectivity of a C8 phase and strong cation exchange for the extraction of basic drugs from biological matrices.<figure class="image"><img style="aspect-ratio:500/150;" src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Phenomenex_Strata_Screen_C_b60bb2b7e4.webp" alt="Phenomenex: Strata Screen-C" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Phenomenex_Strata_Screen_C_b60bb2b7e4.webp 245w," sizes="100vw" width="500" height="150"></figure><ul><li>Surface Area (m²/g): 500, m²/g, 500 m²/g</li><li>Separation Mode: Multi-Mode</li><li>Pore Size: 70</li><li>Particle Size: 55</li><li>Recommended Use: Extraction of basic drugs from biological matrixes</li></ul><h4>Starting Method</h4><h5>Strata Screen-C Strong Cation-Exchange</h5><ul><li>Condition: 1 mL Methanol</li><li>Equilibrate: 1 mL Acidified Water</li><li>Load: Pretreated sample (acidified)</li><li>Wash 1: 1 mL 0.1N HCl in Water</li><li>Wash 2: 1 mL 0.1N HCl in Methanol, dry under vacuum for 2-5 min</li><li>Elute: 1 mL 5 % NH4OH in Methanol</li></ul>Based on 100 mg/1 mL sorbent mass. The above is a convenient starting point for SPE method development, further optimization may be required to tailor the method for your specific extraction needs.

Phenomenex Strata Screen-C GF Solid Phase Extraction (SPE)

Optimized for gravity flow, this sorbent offers hydrophobic selectivity from a C8 phase and strong cation-exchange properties for the extraction of basic drugs.

Strong Cation-Exchange Solid Phase Extraction (SPE)Optimized for gravity flow, this sorbent offers hydrophobic selectivity from a C8 phase and strong cation-exchange properties for the extraction of basic drugs from biological matrixes. Offers the selectivity of Screen-C in a gravity flow particle size for viscous samples.<h4>Starting Method</h4><h5>Strata Screen-C GF Strong Cation-Exchange</h5><ul><li>Condition: 1 mL Methanol</li><li>Equilibrate: 1 mL Acidified Water</li><li>Load: Pretreated sample (acidified)</li><li>Wash 1: 1 mL 0.1N HCl in Water</li><li>Wash 2: 1 mL 0.1N HCl in Methanol, dry under vacuum for 2-5 min</li><li>Elute: 1 mL 5 % NH4OH in Methanol</li></ul>Based on 100 mg/1 mL sorbent mass. The above is a convenient starting point for SPE method development, further optimization may be required to tailor the method for your specific extraction needs.

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