Phenomenex β-Gone β-Glucuronidase Removal 96-well plates and tubes

β-Gone™ offers a simple, fast, and highly targeted method to remove β-Glucuronidase from urine samples prior to analysis. Within 1 minute, with no necessary method development, your samples will be ready for analysis.

Rapid Cleanup of Hydrolyzed Urine

• Increase HPLC/UHPLC Column Lifetime
• Reduce Mass Spec Maintenance
• Maintain the Selectivity of Your HPLC/UHPLC Columm

Available Formats & Protocols:

β-Gone Plus 96-Well Plates Protocol:

• Follow β-glucuronidase vendor specifications and add appropriate amount of enzyme and urine to the well* 
  • Please note: Do not use more than 10% organic solution for the incubation and ensure that  organic solution is added last.
• Add 133 µL of 0.1% Formic acid in Methanol
• Initiate vacuum or positive pressure (2-5 psi) and collect eluent to inject**

β-Gone 96-Well Plates and 1 mL Tubes Protocol:

• Dilute 200 µL of urine hydrolysate with 133 µL of 0.1% Formic acid in Methanol
• Load diluted sample onto plate/tube and apply vacuum  or positive pressure (2-5 psi) and collect eluent
• Inject eluent**

2 mL Centrifuge Tubes Protocol:

• Tap tube prior to use to consolidate sorbent 
• Combine 200 µL of Urine Hydrolysate with 133 µL of 0.1 % Formic acid in Methanol 
• Load into the centrifuge tube, cap, and invert 10x. Vortex for 30 seconds.
• Centrifuge tube at 14000 rpm for 10 min (16000 xg RCF) 
• Collect supernatant to inject for analysis**
   

*For urine samples < 200 µL, use 30 mg β-Gone Plus Plate 
For urine samples  ≥ 200 µL, use 60 mg β-Gone Plus Plate 
**Depending on injection volume, starting %B (organic) in mobile phase, and column dimensions, a dilution of 1:1 with aqueous mobile  phase may be required to prevent peak distortion of early eluting compounds. 

Ordering Information

β-Gone β-Glucuronidase Removal Products

• 8B-S139-TAK: 1 mL Tubes, Recombinant Enzyme, 100/Box
• 8B-S322-DAK: 1 mL Tubes, Non-Recombinant Enzyme, 100/Box
• 8E-S139-TGA: 96-Well Plate, Recombinant Enzyme, 1/Box 
• 8E-S322-DGA: 96-Well Plate, Non-Recombinant Enzyme, 1/Box 
• 8N-S323-TUK: 2 mL Centrifuge Tubes, Recombinant and Non-Recombinant Enzyme, 100/Box 
• 8E-S323-TGA: 96-Well Plate Plus 30 mg/well, Recombinant/Non-Recombinant Enzyme, 1/Box
• 8B-S323-UGA: 96-Well Plate Plus 60 mg/well, Recombinant/Non-Recombinant Enzyme, 1/Box