Phenomenex Strata SAX Solid Phase Extraction (SPE)

Ion-Exchange Solid Phase Extraction Sorbent (SPE)

The quaternary amine group on this sorbent remains positively charged under all conditions, giving a strong anion-exchange mechanism of retention.

Phenomenex: Strata-SAX

• Surface Area (m²/g): -
• Separation Mode: Ion Exchange
• Particle Size: 55
• Recommended Use: Extraction of weakly acidic compounds

Starting Method

Strata SAX – Strong Anion-Exchange

• Condition: 1 mL Methanol
• Equilibrate: 1 mL DI Water
• Load: Pretreated sample, pH 6-7
• Wash 1: 1 mL 25mM Ammonium Acetate Buffer, pH 6-7
• Wash 2: 1 mL Methanol, dry under vacuum for 2-5 min
• Elute: 1 mL 5% Formic Acid in Methanol

Based on 100 mg/1 mL sorbent mass. The above is a convenient starting point for SPE method development, further optimization may be required to tailor the method for your specific extraction needs.