Ask any analytical chemist which hyphenated technique has become indispensable over the past decade, and the answer will almost certainly be the same: tandem chromatography–mass spectrometry. Whether it is <a target="_blank" rel="noopener noreferrer" href="https://www.organomation.com/preparing-samples-for-lc-ms/ms-analysis">LC–MS/MS</a> quantifying a novel biologic’s metabolic fate, or <a target="_blank" rel="noopener noreferrer" href="https://www.organomation.com/preparing-samples-for-gc-ms/ms-analysis">GC–MS/MS</a> chasing per- and polyfluoroalkyl substances (PFAS) down to sub-part-per-trillion levels in municipal water, the combination of chromatographic separation with the specificity of tandem mass detection has become the analytical workhorse of choice across virtually every regulated sector.Below we examine the key forces propelling this expansion, from biopharma pipeline dynamics and PFAS regulatory mandates to AI-driven automation and reshoring of pharmaceutical manufacturing—and explore what each trend means for laboratories managing sample preparation and analytical workflows.<h3>Biopharma: The Engine Room of LC–MS/MS Demand</h3><h4>GLP-1 Agonists and the Metabolomics Gold Rush</h4>The explosive commercial and scientific interest in GLP-1 receptor agonists—<a target="_blank" rel="noopener noreferrer" href="https://www.sepscience.com/advancing-glp-1-peptide-analysis-chromatography-challenges-and-innovations-12163">semaglutide and related peptide therapeutics </a>for diabetes and obesity—has dramatically expanded the analytical demands placed on tandem MS platforms. Discovery programs require precise quantification of peptide drug candidates and endogenous biomarkers across complex biological matrices. These workflows rely on high-sensitivity LC–MS/MS for pharmacokinetic profiling, receptor occupancy studies, and metabolomics characterization. Metabolomics is, in fact, projected to be the fastest-growing application segment in mass spectrometry, driven in part by its growing role in cancer screening, biomarker discovery, and metabolic disease research.I have been spending more time learning about GLP-1s because this is the hot topic that people want to discuss on the Concentrating on <a target="_blank" rel="noopener noreferrer" href="https://open.spotify.com/show/1wZg67rCjoGju4yhqxI3tL?si=n9GLz9zjSCOrVfXPxFPhng&amp;nd=1&amp;dlsi=52868e027aad4d22">Chromatography podcast</a>. With that said, I was astounded looking at CNBC.com during breakfast this morning to see that <a target="_blank" rel="noopener noreferrer" href="https://www.cnbc.com/2026/03/21/glp-1-diets-restaurants-protein-fiber-weight-loss-drugs.html">one in eight Americans are currently taking one of these drugs such as Ozempic</a>.<h4>Antibody–Drug Conjugates and Gene Therapy: Complexity at Every Step</h4>The ascent of antibody–drug conjugates (ADCs) and gene therapies has introduced a new level of analytical complexity. ADCs blend the target specificity of monoclonal antibodies with the cytotoxic potency of small-molecule payloads—and characterizing the linker chemistry, drug-to-antibody ratio (DAR), and conjugate stability demands high-resolution LC–MS/MS at every stage from discovery through QC release testing. Gene therapy vectors require equally rigorous characterization of impurities, aggregates, and empty/full capsid ratios.This translates into a direct need for low-evaporation, high-recovery concentration methods that preserve labile protein structures and maintain sample integrity prior to LC–MS/MS injection. <a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/article/5392">Nitrogen evaporation workflows</a> that minimize oxidative stress and maintain sample temperature are increasingly critical in these pipelines.<h4>Precision Medicine and Multi-Omics Integration</h4>The shift toward precision medicine has accelerated adoption of high-throughput, multi-analyte MS platforms. Proteomics, metabolomics, and lipidomics workflows are converging on a single analytical platform—tandem MS—and generating datasets of a scale and complexity that were unimaginable ten years ago.Make no mistake: the triple quadrupole serves an irreplaceable role in quantitative targeted analysis. At the same time, <a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/products?category=134&amp;manufacturerItems=3">Orbitrap </a>and Q–TOF hybrid platforms are growing rapidly as researchers demand full-scan accurate mass for untargeted discovery.<h3>Environmental Mandates: PFAS Testing Drives Adoption</h3>Environmental testing is something that every person, chemist or not, can understand the importance of. Mounting mandates from the U.S. Environmental Protection Agency and European regulators regarding PFAS—the “forever chemicals” whose extraordinary environmental persistence is matched only by the analytical challenge of detecting them at trace levels—have created immediate, compliance-driven investment in LC–MS/MS infrastructure.<h4>The Regulatory Framework Chemists Need to Know</h4>The EPA has codified a suite of LC–MS/MS-based methods as the analytical standard for PFAS in water and solid matrices:<ul><li><a target="_blank" rel="noopener noreferrer" href="https://blog.organomation.com/blog/epa-533-drying-down-pfas-samples-after-spe">EPA Method 533</a>: Weak anion exchange SPE combined with LC–MS/MS; covers 25 PFAS including short-chain compounds in drinking water.</li><li><a target="_blank" rel="noopener noreferrer" href="https://blog.organomation.com/blog/sample-concentration-epa-method-537-pfas-determination">EPA Method 537.1</a>: Reversed-phase SPE followed by LC–MS/MS; covers 18 PFAS including the GenX compound, with method detection limits in the low ng/L range.</li><li><a target="_blank" rel="noopener noreferrer" href="https://www.agilent.com/en/solutions/environmental/water-testing/pfas-in-water/us-epa-method-8327?srsltid=AfmBOooCY1pcavj7B1PH-75d3HO4BGDglNlhmyTyrpDY3zvUksMO1dlg">EPA Method 8327</a>: LC–MS/MS for selected PFAS in prepared extracts of aqueous and solid matrices.</li><li><a target="_blank" rel="noopener noreferrer" href="https://blog.organomation.com/blog/preparing-diverse-sample-matrices-for-epa-method-1633">EPA Draft Method 1633</a>: The emerging benchmark for PFAS in non-aqueous matrices—aqueous, solid, biosolids, and tissue—quantifying up to 40 PFAS.</li></ul>All of these methods designate LC–MS/MS, specifically LC coupled to triple quadrupole or tandem-in-time platforms operating in negative electrospray ionization mode with multiple reaction monitoring (MRM), as the required or recommended analytical technique. PFAS analysis is, at its core, an LC–MS/MS problem.<h4>The Sample Preparation Bottleneck</h4>Chemists who have worked with PFAS methods understand the particular demands they place on sample preparation. The ubiquity of<a target="_blank" rel="noopener noreferrer" href="https://blog.organomation.com/blog/tackling-pfas-contamination-practical-insights-from-epa-537.1-method-development"> PFAS in laboratory materials</a>—PTFE tubing, some SPE sorbents, standard bench supplies—means that blank contamination control is as analytically demanding as the detection itself. EPA 537.1 requires that sample volumes of approximately 250 mL be extracted and reconstituted in as little as 1 mL, creating a 250:1 concentration factor that can expose even trace contamination in the sample processing chain. The installation of a delay column between the LC pump and autosampler to prevent instrument background interference is now standard practice.This is precisely where robust, <a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/article/5385">inert nitrogen evaporation </a>systems earn their keep. PFAS workflows that use nitrogen-assisted evaporation to concentrate extracts prior to reconstitution benefit from materials that avoid PTFE and other fluoropolymers that can leach PFAS background—a consideration Organomation addresses in its concentration system designs.The analytical community is also moving beyond targeted PFAS analysis. High-resolution accurate mass (HRAM) spectrometry via Orbitrap technology, paired with non-targeted screening workflows, is gaining traction for the identification of the more than 5,000 currently catalogued PFAS compounds and the novel structures that continue to emerge. LC–MS/MS remains the quantitative gold standard; HRAM provides the discovery layer.<h3>Technological Trends Reshaping the LC–MS/MS Workflow</h3><h4>Artificial Intelligence and Automated Data Interpretation</h4>The datasets generated by modern tandem MS platforms—particularly in omics applications—are too large and too complex for conventional manual review. AI-driven spectral deconvolution, automated peak integration, and AI-assisted hypothesis generation are now embedded in the software suites accompanying leading instruments. <a target="_blank" rel="noopener noreferrer" href="https://www.agilent.com/about/newsroom/presrel/2022/17feb-gp22004.html?srsltid=AfmBOop3warRIaEojXM2YyMML6RXfMIDzE-Wm0h-SiKkkocBeeET2m7u">Agilent’s acquisition of Virtual Control’s ACIES </a>software specifically to automate LC–MS and GC–MS data analysis is emblematic of this direction.For laboratory managers, AI integration means smaller specialized teams can operate high-throughput multi-analyte workflows—but it also means that the front-end sample preparation must be even more reproducible, since algorithmic interpretation amplifies inconsistencies introduced upstream.<h4>Online SPE Coupling and “Chromatography Without Walls”</h4>A significant trend is the integration of solid-phase extraction directly into the HPLC flow path—so-called online SPE–HPLC coupling. Systems that combine extraction and chromatographic separation in a single analytical instrument reduce manual transfer steps, minimize the risk of sample loss or contamination, and dramatically increase throughput. For analytes at very low concentrations, online SPE provides the on-column concentration capability that traditional offline preparation achieves with manual evaporation steps.That said, offline sample preparation—including <a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/article/5392">nitrogen-assisted evaporation</a> under controlled conditions, <a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/products/2407">liquid–liquid extraction</a>, and protein precipitation—retains significant advantages in flexibility, matrix handling, and the ability to process sample volumes that exceed online system capacity. The two approaches are increasingly complementary rather than competitive in well-designed analytical laboratories.<h4>High-Purity Solvents and Reference Materials</h4>As detection limits push further into the femtogram range and regulatory methods demand demonstrable blank performance, the quality of analytical consumables—mobile phase solvents, derivatization reagents, reference standards—has moved from background consideration to front-of-mind. Method validation in regulated environments now routinely requires vendor certification of solvent purity at a level that directly interfaces with instrument performance.<h3>The Ever-Present Need for Sample Evaporation</h3>As LC–MS/MS methods push detection limits lower, the analytical burden shifts increasingly upstream. The quality of the concentration, cleanup, and reconstitution steps preceding injection determines whether the instrument’s theoretical sensitivity is achievable in practice.Several specific developments are particularly relevant:<ul><li>ADC and gene therapy characterization workflows require gentle, low-temperature evaporation that preserves protein structure and prevents aggregation prior to LC–MS/MS. Nitrogen evaporation under controlled conditions is the preferred approach.</li><li>PFAS trace analysis demands evaporation systems constructed from PFAS-inert materials, with rigorous attention to avoiding fluoropolymer surfaces throughout the sample path.</li><li>High-throughput pharmaceutical QC driven by reshoring demands parallel processing capability—multi-position evaporators that maintain consistent flow and temperature across all positions simultaneously.</li><li>Multi-omics and metabolomics workflows generate large sample batches that benefit from automated, walk-away evaporation with programmable endpoints to ensure reproducible final volumes across a cohort.</li></ul>Tandem chromatography–mass spectrometry is not merely growing; it is becoming structurally embedded in the analytical workflows of every major regulated industry. The convergence of biopharma complexity, PFAS regulatory mandates, AI-enabled data analysis, and pharmaceutical reshoring is creating a multi-year demand cycle that will require laboratories to invest not only in instruments, but in the entire sample preparation ecosystem that makes those instruments perform.For analytical chemists, the practical takeaway is this: as mass spectrometry sensitivity continues to advance, the sample preparation step becomes the binding constraint on method performance. Whether you are developing a new PFAS method to meet EPA 1633, characterizing an ADC’s drug-to-antibody ratio, or running a 500-sample metabolomics cohort overnight, the reliability and cleanliness of your concentration and cleanup workflow determines the quality of every data point that follows.&nbsp;

The Continued Rise of Tandem Chromatography–Mass Spectrometry

News from LabRulezLCMS Library - Week 27, 2026

🎤Dr. Christopher Reid (Professor of Chemistry and Biochemistry at Bryant University in Rhode Island)In this episode of Concentrating on Chromatography, host Dave Oliva sits down with Chris Reid to explore how chemical biology and LC-MS are being used to tackle one of the most urgent global challenges: antimicrobial resistance.As antibiotic resistance continues to rise—and new drug development struggles to keep pace—researchers are looking beyond traditional approaches. Instead of targeting how bacteria build their cell walls, Chris’s work focuses on how they break them down, opening the door to entirely new antimicrobial strategies.<h4>What You’ll Learn in This Episode:</h4><ul><li>Why antimicrobial resistance is accelerating globally</li><li>What peptidoglycan is and why it’s essential to bacterial survival</li><li>How targeting cell wall degradation differs from traditional antibiotics</li><li>The strategy behind designing small molecule probes and inhibitors</li><li>How LC-MS is used to analyze peptidoglycan fragments and enzyme activity</li><li>Key analytical challenges: complex mixtures, low-abundance species, and structural similarity</li><li>The importance of sample preparation and reproducibility in LC-MS workflows</li><li>How this research could lead to new antibiotic targets</li></ul><h3>Video Transcription</h3><h4>The Growing Challenge of Antibiotic Resistance</h4>According to Reid, antibiotic resistance is increasing across a broad spectrum of bacterial pathogens that were once readily treated with existing therapies. In some cases, clinicians must now rely on only a handful of last-line antibiotics, while resistance is also rising in diseases such as tuberculosis.One of the major obstacles is the limited commercial incentive to develop entirely new antibiotics. Because bacterial resistance can emerge relatively quickly after a drug reaches the market, pharmaceutical companies often favor modifying existing antibiotics or combining them with resistance-modifying agents rather than investing in completely new drug classes. As a result, genuinely novel antibacterial targets remain urgently needed.<h4>Why the Bacterial Cell Wall Remains an Attractive Target</h4>The bacterial cell wall consists primarily of peptidoglycan, a highly cross-linked polymer that provides structural integrity, maintains cell shape, and enables bacterial growth and division.Many widely used antibiotics—including the β-lactam antibiotics such as penicillins—target enzymes responsible for synthesizing or cross-linking peptidoglycan. These pathways have proven highly successful therapeutic targets because peptidoglycan is unique to bacteria and absent from human cells, minimizing unwanted effects on the host.However, most existing antibiotics target only specific stages of peptidoglycan biosynthesis. Reid's research explores another part of this complex pathway: enzymes responsible for degrading the carbohydrate backbone of peptidoglycan during normal bacterial growth and remodeling. These enzymes may represent an underexplored class of antimicrobial targets capable of overcoming existing resistance mechanisms.<h4>Targeting the Glycan Backbone</h4>A key advantage of focusing on the carbohydrate backbone is its remarkable conservation across both Gram-positive and Gram-negative bacteria.Whereas the peptide components of peptidoglycan vary considerably between bacterial species—and frequently acquire mutations associated with antibiotic resistance—the glycan backbone remains highly conserved and tolerates relatively few structural modifications.This biological constraint makes enzymes acting on the glycan backbone particularly attractive targets. Reid's group developed a series of small-molecule probes and inhibitors designed to recognize enzymes known as N-acetylglucosaminidases, which cleave glycosidic bonds within peptidoglycan and generate soluble degradation products.Rather than beginning with purified enzymes, the researchers adopted a top-down phenotypic screening strategy. Candidate compounds were first evaluated for their ability to inhibit bacterial growth before progressively narrowing the list of potential molecular targets through genetic studies, microscopy, enzyme assays, and biochemical characterization. This iterative workflow ultimately identified compounds capable of inhibiting the desired enzyme class while simultaneously providing valuable chemical probes for studying bacterial cell wall biology.<h4>LC-MS as a Central Analytical Tool</h4>Liquid chromatography coupled with mass spectrometry plays a central role throughout the project by enabling detailed characterization of structural changes occurring within peptidoglycan after bacterial cells are treated with candidate inhibitors.Because intact peptidoglycan is an insoluble polymer, sample preparation begins with isolation of the bacterial cell wall through detergent extraction and differential centrifugation. Additional purification steps remove proteins, nucleic acids, and teichoic acids before enzymatic digestion converts the insoluble material into soluble fragments suitable for chromatographic analysis.The resulting digest is analyzed by LC-MS to examine changes in the distribution of peptidoglycan fragments. Chromatographic retention times provide an immediate indication of changes in cross-linking, with larger oligomers such as trimers and tetramers generally eluting later than monomeric fragments. Mass spectrometry then confirms the identity of these fragments by measuring their intact molecular masses, while tandem MS experiments can provide further structural information when required.<h4>Sample Preparation Determines Data Quality</h4>Reid emphasized that sample preparation represents one of the most critical—and challenging—stages of the entire workflow.Because peptidoglycan analysis involves multiple purification, digestion, and reduction steps, even minor procedural differences between samples can introduce analytical artifacts that complicate biological interpretation.Maintaining strict consistency throughout sample preparation is therefore essential. Appropriate controls must accompany every experiment, ensuring that observed chromatographic and mass spectrometric differences truly reflect biological responses rather than experimental variability. The complexity of bacterial cell wall composition further increases the analytical challenge, as subtle differences in amino acid substitutions and low-abundance cross-linked species can complicate LC-MS data interpretation.<h4>Chemical Biology for Future Antibiotic Discovery</h4>Although the identified compounds may eventually serve as starting points for antibiotic development, Reid views them primarily as chemical biology tools.By selectively inhibiting individual enzymes, these probes allow researchers to investigate previously unexplored aspects of bacterial cell wall remodeling that cannot easily be studied using classical genetic approaches alone.The research team is now expanding this strategy by combining their probes with proximity-labeling proteomics and mass spectrometry, aiming to map protein interaction networks associated with bacterial cell wall degradation. Such studies may reveal entirely new antibacterial targets and provide deeper insight into the biological processes governing bacterial growth and division.Ultimately, the work illustrates how integrating synthetic chemistry, microbiology, molecular biology, and advanced LC-MS can accelerate the search for innovative therapeutic strategies against antimicrobial resistance. As existing antibiotics become progressively less effective, multidisciplinary approaches such as these may play an increasingly important role in identifying the next generation of antibacterial targets.<blockquote>This text has been automatically transcribed from a video presentation using AI technology. It may contain inaccuracies and is not guaranteed to be 100% correct.</blockquote><blockquote><h4>Concentrating on Chromatography Podcast</h4>Dive into the frontiers of chromatography, mass spectrometry, and sample preparation with host David Oliva. Each episode features candid conversations with leading researchers, industry innovators, and passionate scientists who are shaping the future of analytical chemistry. From decoding PFAS detection challenges to exploring the latest in AI-assisted liquid chromatography, this show uncovers practical workflows, sustainability breakthroughs, and the real-world impact of separation science. Whether you’re a chromatographer, lab professional, or researcher you'll discover inspiring content!You can find Concentrating on Chromatography Podcast in podcast apps:<ul><li><a target="_blank" rel="noopener noreferrer" href="https://open.spotify.com/show/1wZg67rCjoGju4yhqxI3tL">Spotify</a></li><li><a target="_blank" rel="noopener noreferrer" href="https://podcasts.apple.com/tz/podcast/concentrating-on-chromatography/id1788429185">Apple podcast</a></li><li><a target="_blank" rel="noopener noreferrer" href="https://podscan.fm/podcasts/concentrating-on-chromatography">podscan</a></li></ul>and on <a target="_blank" rel="noopener noreferrer" href="https://www.youtube.com/@ChromatographyTalk/videos">YouTube channel</a></blockquote>

 Fighting Antibiotic Resistance with LC-MS: Targeting Bacterial Cell Walls

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Organomation, founded in 1959, designs and manufactures high-quality nitrogen evaporators and extraction systems. Known for innovation and durability, their lab instruments are used globally for efficient sample preparation with strong customer support.

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Waters CERTIFIED HPLC Vials

Reducing failed assays from unknown contaminant peaks and limiting instrument downtime from out of specification vials is a priority for your lab. Waters sample vials can help you do just that.

<div><div><h4>Meet your analytical challenges with the right vial.</h4></div></div><div><div>The right vial.&nbsp;A world of difference.Reducing failed assays from unknown contaminant peaks and limiting instrument downtime from out of specification vials is a priority for your lab. Waters sample vials can help you do just that.All Waters sample vials are held to the tightest dimensional tolerances. Manufactured via a 2-, 3-, or 4-step certification process, we ensure that Waters Certified Vials meet and maintain specified vial dimensions, levels of cleanliness and adsorption properties from batch to batch.&nbsp;Find the right vial for your application and autosampler - use our <a target="_blank" rel="noopener noreferrer" href="https://find.waters.com/VialSelector/">Vial Selector</a> to find your perfect match.<img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Waters_CERTIFIED_Vials_118649175c.jpg" alt="Waters CERTIFIED Vials: Table 1. Vials for Routine and Advanced Analyses." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Waters_CERTIFIED_Vials_118649175c.jpg 245w," sizes="100vw" width="1226" height="754"><h3>Waters LCMS CERTIFIED</h3>✅ Dimensional Test✅ Septum GC Test✅ HPLC UV Test✅ MS Scan<ul><li>Low MS interferences from known residues &amp; polymers</li><li>For routine QC and LC-MS analysis with lower analyte concentrations from 10-100s ng/ml.</li></ul><h3>Waters TruViewTM LCMS CERTIFIED</h3>✅ Dimensional Test✅ Septum GC Test✅ HPLC UV Test✅ MS Scan✅&nbsp;Low Adsorption Test<div><div><ul><li>Lowest MS interferences from known residues &amp; polymers</li><li>For high-end LC-MS analysis and quantification of analytes with a concentration of below 1 ng/ml with reduced adsorption of polar analytes.</li></ul><h3>Waters CERTIFIED</h3>✅ Dimensional Test✅ Septum GC Test✅ HPLC UV Test<div><div><ul><li>No UV interferences caused caused by manufacturing and packaging residues</li><li>For routine QC LC-UV analysis&nbsp;of compounds in µg/mL&nbsp;concentrations.&nbsp;</li></ul><h3>MAXPEAK QuanRecovery</h3><ul><li>For high recovery peptide and protein quantification workflows. Reduces sample losses due to non-specific binding (NSB) and ionic interactions.</li></ul><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Waters_CERTIFIED_Vials_2_4fba532335.jpg" alt="Waters CERTIFIED Vials: Vials Shapes." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Waters_CERTIFIED_Vials_2_4fba532335.jpg 245w," sizes="100vw" width="1261" height="617"><div><div><h3>Control data quality risks and reduce unexpected or ghost peaks</h3>Don’t let the presence of background noise impact your results, causing data quality risks and unexpected or ghost peaks. Background noise can come from many factors.&nbsp;One of the most important factors are vial and septa cleanliness, which introduces contamination peaks. Ghost peaks are introduced by analyte degradation that can be triggered by significant pH changes in the diluent. In this chromatogram, the top shows normal behavior, and the bottom chromatogram shows ghost peaks.Waters Certified vials are cleanliness tested so you don’t have to worry about contaminant peaks. The TruView pH Control Vials are certified to minimize and control a pH shift away from the neutral.<img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Waters_CERTIFIED_Vials_4_7749301ba1.jpg" alt="Waters CERTIFIED Vials: Prevent the appearance of unexpected peaks during LC/UV analysis with Waters LC/GC Certified Vials as a control strategy." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Waters_CERTIFIED_Vials_4_7749301ba1.jpg 245w," sizes="100vw" width="1024" height="535"><h3>More time on development. Less on troubleshooting</h3>Stop losing valuable development time on troubleshooting due to analyte loss from non-specific adsorption. Waters TruView pH Control LCMS Certified Vials have the lowest adsorptive losses on the market, and you can rely on them to provide consistency from batch to batch and vial to vial. This chromatogram shows a comparison of adsorptive loss of chlorhexidine in a variety of vials.<img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Waters_CERTIFIED_Vials_5_d3e42fa7f2.jpg" alt="Waters CERTIFIED Vials: Comparison of adsorptive loss of chlorhexidine acetate in a variety of vials." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Waters_CERTIFIED_Vials_5_d3e42fa7f2.jpg 245w," sizes="100vw" width="1024" height="633"><div><div><h3>Ideal for demanding MS work</h3>LCMS Certified Vials are suited for analyte concentrations in the 100s ng/mL concentration. Waters LCMS Certified Vials are tested by MS scans from 250 to 1000 m/z with specifications set on the total ion count and presence of clusters in the high mass range. This chromatogram shows a comparison of typical MS scans of Waters LCMS Certified Vials to vials of other sources and clean solvents.<img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Waters_CERTIFIED_Vials_3_4869fbf57a.jpg" alt="Waters CERTIFIED Vials: Comparing typical MS scans of Waters LCMS Certified Vials, with vials from other sources." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Waters_CERTIFIED_Vials_3_4869fbf57a.jpg 245w," sizes="100vw" width="1024" height="406"><div><div><h3>Stay in control of your samples' pH</h3>The analysis of low abundance species, pH sensitive analytes or not yet well understood compound mixtures benefit from consistent and well controlled vial surfaces. The glass surface of TruView pH Control LCMS Certified Vials minimizes pH shifts away from the neutral and thus eliminates unwanted reactions and resulting ghost peaks.&nbsp;Ghost peaks are very difficult to troubleshoot.Waters TruView pH Control LCMS Certified vials were used to analyze two compounds, drospirenone and lovastatin. The TruView pH Control LCMS Certified Vials show little to no degradant formed over the time tested. Standard glass vials showed considerable degradant formation over the same testing time with irreproducible formation of the degradant across different vials.&nbsp;<img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Waters_CERTIFIED_Vials_6_4dc330d849.jpg" alt="Waters CERTIFIED Vials: UV peak areas of lovastatin degradant across replicate vials and different vial types. Error bars indicate standard deviation for the replicate injection peak area." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Waters_CERTIFIED_Vials_6_4dc330d849.jpg 245w," sizes="100vw" width="1024" height="605"><div><div><h3>Reducing adsorption starts here</h3>Inconsistent analytical results from small molecule analyte adsorption onto the surface of glass vials can be a problem at today’s low analyte concentrations. Some scientists have even experienced total analyte loss with samples stored in vials for an extended time. The vial chemistry, specifically the&nbsp;glass surface, is the major contributor to this problem.Polar analytes&nbsp;may adsorb to the glass vial surface by polar-polar interaction and/or ion exchange mechanisms, thereby reducing the overall amount of sample in solution. TruView Certified LCMS Vials&nbsp;reduce this problem dramatically due to a stringent manufacturing process. The process yields a glass surface that has a lower concentration of free ions on the surface.<img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Waters_CERTIFIED_Vials_8_064003b933.jpg" alt="Waters CERTIFIED Vials: Nortriptyline at 1 ppb concentration in several vials. The tall peak is the initial injection; the smaller peak (overlaid) is the four-hour injection. The first two chromatograms are competitor’s vials. The third chromatogram is for the Waters TruView LCMS Certified vial. " srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Waters_CERTIFIED_Vials_8_064003b933.jpg 245w," sizes="100vw" width="1912" height="1182"><div><div><h3>No analyte left behind</h3>Instrument development is yielding more sensitive mass spectrometers. With more sensitive instruments, analytes can be detected at pg/mL levels. At these concentrations, understanding adsorption issues is critical for good quantitative results. We tested adsorption loss for the major manufacturers and saw high sample loss across manufacturers and clear and amber glass types – with significantly less loss with Waters TruView Autosampler Vials.<h4>Average Analyte Loss</h4>Manufacturer: Clear Glass / Amber Glass<ul><li>Vendor A: 55% / 69%</li><li>Vendor B: 25% / 25%</li><li>Vendor C: 54% / 74%</li><li>Vendor D: 18% / 59%</li><li>Vendor E: 75% / Not tested</li><li>Waters TruView LCMS Certified Vial: 1% / 6%</li></ul></div></div></div></div></div></div></div></div></div></div></div></div></div></div></div></div>

Waters SARS-CoV-2 LC-MS Kit (RUO)

Advance virus research with Waters' RUO LC-MS kit for SARS-CoV-2. Detect and quantify NCAP peptides with high sensitivity and reproducibility using SISCAPA antibody enrichment and automation-ready workflows.

<h4>Detect and quantify SARS-CoV-2 NCAP peptides to advance virus research</h4>The global COVID-19 pandemic created an unprecedented need to quickly deploy emergency tests for SARS-CoV-2 infection. Now, scientists can harness the flexibility and reproducibility of LC-MS to advance critical SARS-CoV-2 research.Waters SARS-CoV-2 LC-MS Kit (RUO) is a Research Use Only kit to directly detect and quantify SARS-CoV-2 signature nucleocapsid peptides (NCAPs) without the need to amplify the target analytes – so you can ensure accuracy, reproducibility, and sensitivity for your most critical research.&nbsp;<img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Waters_SARS_Co_V_2_LC_MS_Kit_RUO_2_50b4ae9b00/Waters-SARS-CoV-2-LC-MS-Kit-(RUO)-2.jpg" alt="Waters: SARS-CoV-2 LC-MS Kit (RUO)" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Waters_SARS_Co_V_2_LC_MS_Kit_RUO_2_50b4ae9b00/thumbnail_Waters-SARS-CoV-2-LC-MS-Kit-(RUO)-2.jpg 234w," sizes="100vw" width="660" height="440"><h4>Overview</h4><ul><li>Achieve reproducible results between and within labs &nbsp;</li><li>Ensure high accuracy and analytical sensitivity to enable critical research &nbsp;</li><li>Empower research and development of pioneering SARS-CoV-2 testing with an alternative and complementary technology to common infectious pathogen research &nbsp;</li><li>Enable broader research with an LC-MS method with the potential to be multiplexed with other peptides and biomarkers&nbsp;</li></ul>* This kit is for research use only and has not been approved for use in clinical diagnostic procedures. This RUO kit has not been tested with clinical samples. The SARS-CoV-2 kit for SARS-CoV-2 nucleocapsid peptides is not available in all countries, please contact your Waters representative for more details.Recommended Use: For the detection and quantification of SARS-CoV-2 signature NCAP peptides.<h4>LC-MS enables cutting-edge SARS-CoV-2 research</h4>The Waters SARS-CoV-2 LC-MS Kit (RUO) is a Research Use Only kit intended for the quantitative measurement of three proteolytic peptides derived from the SARS-CoV-2 Nucleocapsid (NCAP) protein. The method has been optimized on an <a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/products/199">ACQUITY UPLC I-Class PLUS</a> and <a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/products/195">Xevo TQ-XS System</a> and is also intended for use with the <a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/products/208">Andrew+ Pipetting Robot</a>. Optimization on other instrument platforms is possible. &nbsp;Further investigation is needed to collect objective evidence in promising research applications, however, based on the properties of the analytical test method, the SARS-CoV-2 LC-MS Kit might be leveraged for the following research studies: &nbsp;<ul><li>Peptide concentration to quantitate SARS CoV-2 signature peptides at different stages of infection&nbsp;</li><li>Research of infection and long-term effects through peptide quantitation&nbsp;</li></ul>Future pandemic preparedness studies and research of signature peptide-based biomarkers unique to other infections <img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Waters_The_SARS_Co_V_2_LC_MS_Kit_RUO_allows_for_easy_sample_preparation_sample_enrichment_and_sample_detection_f0aca034ad.jpg" alt="Waters: The SARS-CoV-2 LC-MS Kit (RUO) allows for easy sample preparation, sample enrichment, and sample detection." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Waters_The_SARS_Co_V_2_LC_MS_Kit_RUO_allows_for_easy_sample_preparation_sample_enrichment_and_sample_detection_f0aca034ad.jpg 234w," sizes="100vw" width="1024" height="682"><h4>Achieve confidence in results</h4>Ensure accurate and consistent results with highly analytically selective antibody magnetic bead enrichment. With the innovative SISCAPA® anti-peptide antibody enrichment sample preparation method from SISCAPA Assay Technologies, Inc. in the SARS-CoV-2 LC-MS Kit (RUO), you can improve the performance of mass spectrometry for measurement of pre-selected protein targets.With the SARS-CoV-2 LC-MS Kit (RUO) and SISCAPA, you can achieve the analytical sensitivity you need to detect low levels of SARS-CoV-2 peptides from complex digested samples with stable isotope standards and capture by anti-peptide antibodies. Increase laboratory efficiency with all the components your busy research laboratory needs to quickly deploy the supplied method - all in one package.<img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Waters_The_SARS_Co_V_2_method_minimizes_sample_media_interference_providing_greater_flexibility_for_the_analysis_of_multiple_research_samples_fe3a56c686.jpg" alt="Waters: The SARS-CoV-2 method minimizes sample media interference, providing greater flexibility for the analysis of multiple research samples." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Waters_The_SARS_Co_V_2_method_minimizes_sample_media_interference_providing_greater_flexibility_for_the_analysis_of_multiple_research_samples_fe3a56c686.jpg 234w," sizes="100vw" width="1024" height="682"><h4>Ensure reproducibility with trusted chemistries and consumables</h4>Ensuring reproducible quantitation from sample-to-sample, run-to-run, and lab-to-lab provides the confidence you need for critical research decisions. Ensuring reproducibility starts with minimizing variability risks, including the risk of unpredictable non-specific binding of peptides to surfaces. The SARS-CoV-2 LC-MS Kit uses QuanRecovery Plates with MaxPeak HPS and a MaxPeak Premier Peptide Column, minimizing non-specific binding of peptides to surfaces while enhancing recovery and signal.<img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Waters_Analytical_sensitivity_of_the_ADE_AYN_and_NPA_peptides_in_VTM_at_2_3_5_10_and_20_amol_L_following_antibody_enrichment_145d5249ef.jpg" alt="Waters: Analytical sensitivity of the ADE, AYN, and NPA peptides in VTM at 2, 3, 5, 10, and 20 amolµL following antibody enrichment." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Waters_Analytical_sensitivity_of_the_ADE_AYN_and_NPA_peptides_in_VTM_at_2_3_5_10_and_20_amol_L_following_antibody_enrichment_145d5249ef.jpg 234w," sizes="100vw" width="1024" height="682"><h4>Let Andrew+ pipette for you</h4>Save valuable time and focus on your research with reliable and reproducible sample preparation using the Andrew+ Pipetting Robot. The SARS-CoV-2 LC-MS Kit (RUO) comes in an automation-friendly format with liquid handling protocols for the Andrew+ Pipetting Robot available on OneLab.<img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Waters_SARS_Co_V_LC_MS_Kit_RUO_automation_protocol_available_for_Andrew_Pipetting_Robot_It_provides_a_flexible_automation_platform_easily_adaptable_to_multiple_workflows_8dca9a99e0.jpg" alt="Waters: SARS-CoV-LC-MS Kit (RUO) automation protocol available for Andrew + Pipetting Robot. It provides a flexible automation platform easily adaptable to multiple workflows." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Waters_SARS_Co_V_LC_MS_Kit_RUO_automation_protocol_available_for_Andrew_Pipetting_Robot_It_provides_a_flexible_automation_platform_easily_adaptable_to_multiple_workflows_8dca9a99e0.jpg 234w," sizes="100vw" width="1024" height="682">

Watrex Deltasil 100 C18 HPLC columns

Need a high quality column at an affordable price? Deltasil™ columns are filled with standard reversed phase for a wide range of applications.

<a target="_blank" rel="noopener noreferrer" href="https://watrex.com/?s=watrex+deltasil+100+C18&amp;post_type=product&amp;dgwt_wcas=1?utm_source=labrulez&amp;utm_medium=propagace&amp;utm_campaign=labrulez_produkty">Watrex DeltaSil™ 100 C18</a> column is a standard reversed phase for wide field of applications in analysis of drugs, organic compounds, peptides and others.Stationary phase is based on synthetic 5 μm silica-gel with 100 Å pores and 15% carbon content. The column has a good peak symmetry and efficiency.Theoretical plate count value for non-polar compounds, such as naphthalene, reaches up to 90.000 theoretical plates per meter.A wide range of column sizes is available, with additional dimensions available on request.We are committed to the sustainability of our columns and therefore also offer backfill.<img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Watrex_Watrex_Deltasil_HPLC_column_Mobile_phase_85_ACN_Flowrate_0_65_mlmin_Pressure_54_bar_Detection_254_nm_Injection_2_ml_de7068538f.jpg" alt="Watrex: Watrex Deltasil HPLC column (Mobile phase 85% ACN; Flowrate 0.65 mlmin; Pressure 54 bar; Detection 254 nm; Injection 2 μl)"><h3>Ordering information:</h3><h5>PN/Component:</h5><ul><li>WI02DC181254 Watrex Deltasil™ 100 C18, 5 μm, 125x4 mm column (Price 234 € ex VAT)</li><li>WI02DC181504 Watrex Deltasil™ 100 C18, 5 μm, 150x4 mm column (Price 234 € ex VAT)</li><li>WI02DC1815046 Watrex Deltasil™ 100 C18, 5 μm, 150x4,6 mm column</li><li>WI02DC182504 Watrex Deltasil™ 100 C18, 5 μm, 250x4 mm column (Price 296 € ex VAT)</li><li>WI02DC1825046 Watrex Deltasil™ 100 C18, 5 μm, 250x4,6 mm column</li><li>WI02DC182508 Watrex Deltasil™ 100 C18, 5 μm, Semi-prep 250x8 mm column (Price 482 € ex VAT)</li></ul>

Watrex Polymer IEX HPLC columns

Do you need HPLC columns for the determination of organic acids, alcohols and sugars? Columns filled with a monodisperse sorbent based on sulfonated styrenedivinylbenzene.

<a target="_blank" rel="noopener noreferrer" href="https://watrex.com/?s=watrex+polymer+iex&amp;post_type=product&amp;dgwt_wcas=1?utm_source=labrulez&amp;utm_medium=propagace&amp;utm_campaign=labrulez_produkty">Watrex Polymer IEX™</a> is the Europe’s leading sorbent for ion exclusion analysis of alcohols, carboxylic acids, sugars, and polysacharides<ul><li>H⁺, Ca²⁺, Pb²⁺ and Na⁺ forms</li><li>Unique monodispersed sorbent based on sulfonated polystyrene</li><li>Bulk packing materials available</li></ul><h3>H form</h3>The hydrogen form is suitable for organic acids, alcohols and some sugars in natural materials. The column is particularly suitable for the analysis of fruit drinks, wine, etc. Its great advantage is the high stability of retention times and the wide concentration range of analytes.<img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Watrex_Column_250x8mm_IEX_H_4da682fa13.jpg" alt="Watrex: Column 250x8mm IEX H"><h3>Ca form</h3>The calcium form is characterized by good selectivity for the separation of mono- and disaccharides in natural materials. The column must be protected from anions forming insoluble calcium salts (phosphates, sulphates, etc.) and cations with a higher affinity for the sorbent than calcium ions.<img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Watrex_Column_250x8mm_IEX_Ca_b840dddcce.jpg" alt="Watrex: Column 250x8mm IEX Ca"><h3>Pb form</h3>The lead form is suitable for the separation of mono- and disaccharides in natural materials. The column must be protected from anions forming insoluble lead salts (phosphates, sulphates, etc.) and cations with a higherUse the appropriate pre-column to extend the life of the analytical column.<h3>Ordering information:</h3><h5>PN/Component:</h5><ul><li>WI020223 Watrex 300x8 mm Polymer IEX H form, 8 um</li><li>WI020213 Watrex 300x8 mm Polymer IEX Ca form, 8 um</li><li>WI020253 Watrex 300x8 mm Polymer IEX Pb form, 8 um</li><li>WI02022 Watrex 250x8 mm Polymer IEX H form, 8 um (Price 926 € ex VAT)</li><li>WI02021 Watrex 250x8 mm Polymer IEX Ca form, 8 um (Price 926 € ex VAT)</li><li>WI02025 Watrex 250x8 mm Polymer IEX Pb form, 8 um (Price 926 € ex VAT)</li><li>WI02122 Guard column insert 10x4.0 mm PEEK Polymer IEX H 8 um, 10 pcs</li><li>WI02121 Guard column insert 10x4.0 mm PEEK Polymer IEX Ca 8 um, 10 pcs</li><li>WI029012 Guard column holder (for 10x4.0 mm PEEK insert)</li></ul>

Watrex IonPlus HPLC columns

Do you perform cation or anion analysis and need the right column at an affordable price? Our Watrex IonPlus™ columns are just what you are looking for!

Watrex IonPlus™ are special columns for ion chromatography. The matrix used is styrene-divinylbenzene based and can withstand higher organic modifier content in the mobile phase.<h3><a target="_blank" rel="noopener noreferrer" href="https://watrex.com/obchod/chromatography/hplc-columns/watrex-columns/ion-exchange/watrex-ionplus-cs19-ic-column-250x46mm/?utm_source=labrulez&amp;utm_medium=propagace&amp;utm_campaign=labrulez_produkty">Watrex IonPlus™ CS19</a></h3><ul><li>Styrene-divinylbenzene, 55% cross-linking (7 μm particle i.d.)</li></ul>The Watrex IonPlus™ CS19 is an optimized column designed for the separation of common cations, small polar amines and moderately hydrophobic and polyvalent cations using simple aqueous eluents and elevated temperatures. They provide excellent separation of alkali metals, alkaline earth metals, alkylamines, alkanolamines and biogenic amines.<img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/HPLC_kolona_Watrex_Ion_Plus_CS_19_738c29ff57/HPLC-kolona-Watrex-IonPlus-CS19.png" alt="Watrex: Separation of anions in a Watrex IonPlus CS19 HPLC column"><h3><a target="_blank" rel="noopener noreferrer" href="https://watrex.com/obchod/chromatography/hplc-columns/watrex-columns/ion-exchange/watrex-ionplus-as19-ic-column-250x46mm/?utm_source=labrulez&amp;utm_medium=propagace&amp;utm_campaign=labrulez_produkty">Watrex IonPlus™ AS19</a></h3><ul><li>Styrene-divinylbenzene, 55% cross-linking (12 μm particle i.d.).</li></ul>Watrex IonPlus™ AS19 is a column analyzing common inorganic anions including fluorides, chlorites, bromates, chlorides, nitrates, phosphates and sulphates in drinking, underground and waste water or other matrices.<h3><a target="_blank" rel="noopener noreferrer" href="https://watrex.com/obchod/chromatography/hplc-columns/watrex-columns/ion-exchange/watrex-150x4mm-ic-anion-i-7um/?utm_source=labrulez&amp;utm_medium=propagace&amp;utm_campaign=labrulez_produkty">Watrex IC Anion I</a></h3><ul><li>Styrene-divinylbenzene (7 μm particle i.d.)</li></ul><h3>Ordering information:</h3><h5>PN/Component:</h5><ul><li>WCS19250046 Watrex IonPlus™ CS19 IC Column 250x4,6mm, 7 um (Price 1 169 € ex VAT)</li><li>WCS19150030 Watrex IonPlus™ CS19 IC Column 150x3mm, 7 um</li><li>WCS19150040 Watrex IonPlus™ CS19 IC Column 150x4mm, 7 um</li><li>WCS19250030 Watrex IonPlus™ CS19 IC Column 250x3mm, 7 um</li><li>WAS19250046 Watrex IonPlus™ AS19 IC Column 250x4,6mm, 7 um (Price 1 090 € ex VAT)</li><li>WAS19150030 Watrex IonPlus™ AS19 IC Column 150x3mm, 7 um</li><li>WAS19150040 Watrex IonPlus™ AS19 IC Column 150x4mm, 7 um</li><li>WAS19250030 Watrex IonPlus™ AS19 IC Column 250x3mm, 7 um</li><li>WAS1925046 Watrex IonPlus™ AS19 IC Column 250x4.6mm, 12 μm</li><li>WI021540 Watrex IC Anion I 150x4mm, 7 μm (Price 731 € ex VAT)</li><li>CAT12546 WATREX 125×4.6mm Universal Cat II, 7um (Price 584 € ex VAT)</li></ul>

Watrex DeltaGel GPC/SEC columns

Do you need a GPC column for SEC chromatography in organic solvents at an affordable price? Our Watrex Deltagel™ are just what you are looking for!

Watrex DeltaGel™ are special mixed-bed GPC columns for SEC chromatography in organic solvents.The columns are based on polystyrene and are intended especially for separation of synthetic polymers in organic solvents.We offer 4 variants of columns that differ in their separation range.Columns are available in various sizes for both analytical and preparative applications.<h3>Linear range:</h3><ul><li>DeltaGel Mixed-E (0 - 33 000 Da)</li><li>DeltaGel Mixed-D (1 000 - 200 000 Da)</li><li>DeltaGel Mixed-C (5 000 - 500 000 Da)</li><li>DeltaGel Mixed-B (20 000 - 7 000 000)</li></ul><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Watrex_Delta_Gel_GPC_SEC_kolony_7dfd459a06/Watrex-DeltaGel-GPC-SEC-kolony.jpg" alt="Watrex: DeltaGel GPC SEC columns for the separation of synthetic polymers"><h3>Ordering information:</h3><h5>PN/Component:</h5><ul><li>DGB.25020 Watrex 250x20 mm DeltaGel™ Mixed-B, 5 um</li><li>DGC.25020 Watrex 250x20 mm DeltaGel™ Mixed-C, 5 um</li><li>DGD.25020 Watrex 250x20 mm DeltaGel™ Mixed-D, 5 um</li><li>DGE.25020 Watrex 250x20 mm DeltaGel™ Mixed-E, 5 um</li><li>DGB.3008 Watrex 300x8 mm DeltaGel™ Mixed-B, 5 um (Price 1 269 € ex VAT)</li><li>DGC.3008 Watrex 300x8 mm DeltaGel™ Mixed-C, 5 um (Price 1 269 € ex VAT)</li><li>DGD.3008 Watrex 300x8 mm DeltaGel™ Mixed-D, 5 um (Price 1 269 € ex VAT)</li><li>DGE.3008 Watrex 300x8 mm DeltaGel™ Mixed-E, 5 um (Price 1 269 € ex VAT)</li><li>DGB.25020G WATREX 30x20 mm DeltaGel™ Mixed-B, 5um, Guard Column</li><li>D2K4101927 Derivatizating agents AQC 5x3 mg</li><li>DGB.0508 WATREX 50×8mm DeltaGel 5 um, Guard column (Price 390 € ex VAT)</li></ul>

Thermo Scientific QuEChERS Sample Preparation Kits

QuEChERS is a versatile sample preparation method for analyzing pesticides, veterinary drugs, and other residues in various sample types before GC-MS or LC-MS analysis.

<div><div><div><h3>QuEChERS sample preparation—now easier, more convenient, and affordable</h3>QuEChERS (Quick, Easy, Cheap, Effective, Rugged, and Safe)is a sample preparation method that was initially developed in 2003 for analyzing veterinary drugs in food. The method was also tested for pesticide residue analysis in plant material with great success. In 2003, the detailed method was published in the journal AOAC International. Since then, the QuEChERS method has evolved into a sample preparation technique used on a variety of analytes in an expanding number of sample types before analysis by <a target="_blank" rel="noopener noreferrer" href="https://www.thermofisher.com/cz/en/home/industrial/mass-spectrometry/gas-chromatography-mass-spectrometry-gc-ms.html">GC-MS</a> or <a target="_blank" rel="noopener noreferrer" href="https://www.thermofisher.com/cz/en/home/industrial/mass-spectrometry/liquid-chromatography-mass-spectrometry-lc-ms.html">LC-MS</a>.Thermo Scientific QuEChERS kits contain pre-packaged, ready-weighed salts, sorbents, and buffers to streamline your workflow and minimize the potential for error. The kits are grouped according to your analytes of interest, sample matrix, and preferred QuEChERS method:<ul><li>Original 2003 Method</li><li>AOAC 2007.01 Method</li><li>EN 15662 Method</li><li>NY/T 1380-2007 Method</li></ul>Complete packages—including both extraction and clean-up kits—are available. Extraction-only and cleanup-only kits are also offered to suit your laboratory’s sample preparation needs. These pre-packaged kits save time and money, make QuEChERS procedures easier and more convenient, and ensure quality to yield reproducible results and excellent recoveries for a wide variety of analytes.<img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Thermo_Scientific_Qu_E_Ch_ERS_Sample_Preparation_Kits_2_1e99f0c81b.jpg" alt="Thermo Scientific QuEChERS Sample Preparation Kits" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Thermo_Scientific_Qu_E_Ch_ERS_Sample_Preparation_Kits_2_1e99f0c81b.jpg 217w," sizes="100vw" width="891" height="640"><h3>Typical QuEChERS workflow</h3><div><div><div><div><div>As shown in the workflow diagram below, the QuEChERS method has four basic steps:<ol><li>Homogenize sample (fruit or vegetable)</li><li>Extract analytes of interest from a sample using extraction salts (pesticide residues, veterinary drugs, and/or mycotoxins)</li><li>Clean-up the resulting supernatant using dispersive solid phase extraction (dSPE)</li><li>Analyze by GC-MS or LC-MS</li></ol><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Thermo_Scientific_Qu_E_Ch_ERS_Sample_Preparation_Kits_496491ceff.jpeg" alt="Thermo Scientific QuEChERS Sample Preparation Kits" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Thermo_Scientific_Qu_E_Ch_ERS_Sample_Preparation_Kits_496491ceff.jpeg 245w," sizes="100vw" width="1170" height="706"><div><h3>QuEChERS products</h3></div><div><h4>QuEChERS extraction kits for original 2003 method</h4></div><div>The original QuEChERS method (Anastassiades et al, 2003) was developed by Steven J. Lehotay and Michelangelo Anastassiades in 2003 for the determination of pesticide residues in fruits and vegetables. The original method was created to analyze non-base-sensitive compounds and uses a sodium chloride buffer to reduce polar interferences and enhance extraction. Buffering is an important part of the QuEChERS procedure because it allows for analysis of pesticides and other residues that display pH dependence in their recoveries, depending on the sample matrix. Three different extraction kits are available to support the original 2003 QuEChERS method.<h5>Chemicals used in the original 2003 method</h5></div><ul><li>Acetonitrile: Used to perform single-phase extraction (SPE) on a sample</li><li>Anhydrous MgSO₄: Induces phase separation between water content in sample and acetonitrile layer during liquid-liquid partitioning step; removes remaining water from sample during dispersive solid phase extraction (dSPE) step</li><li>NaCl: Induces phase separation between water content in sample and acetonitrile layer during liquid-liquid partitioning step; removes residual water from sample; reduces polar interference and enhances extraction</li><li>PSA: Primary secondary amine, used to remove polar matrix components, citric co-extractives, and free fatty acids, from sample during dispersive solid phase extraction (dSPE) step</li></ul><div><h4>QuEChERS extraction and clean-up kits for AOAC 2007.01 method</h4>The AOAC 2007.01 QuEChERS Method was developed by Steven J. Lehotay and his co-workers, who modified the original 2003 method to accommodate the use of acetate buffering at higher concentration for greater buffering strength. Buffering is an important part of the QuEChERS procedure because it allows for analysis of pesticides and other residues that display pH dependence in their recoveries, depending on the sample matrix. These extraction and clean-up products here support the AOAC 2007.01 method.<div><h5>Chemicals used in the AOAC 2007.01 method</h5><ul><li>Acetonitrile: Used to perform single-phase extraction on a sample</li><li>Anhydrous MgSO₄: Induces phase separation between water content in sample and acetonitrile layer during liquid-liquid partitioning step; removes remaining water from sample during dispersive solid phase extraction (dSPE) step</li><li>NaOAc: Buffers the sample to stabilize pH</li><li>PSA: Primary secondary amine, used to remove polar matrix components, citric co-extractives, and free fatty acids, from sample during dispersive solid phase extraction (dSPE) step</li><li>C18: Removes fats, sterols, and other non-polar interferences from sample</li><li>GCB: Graphitized carbon black, removes pigment (not recommended for use with planar pesticides)</li></ul><div><h4>QuEChERS extraction and clean-up kits for EN 15662 method</h4>The extraction and clean-up products here support the EN 15662 method. Widely used in Europe, the EN 15662 method was developed by Michelangelo Anastassiades and co-workers, who modified the original 2003 method to accommodate the use of citrate buffering at a relatively low buffering capacity. This variation uses a sodium chloride buffer to minimize polar interferences in addition to sodium citrate dihydrate and disodium citrate sesquihydrate buffers. Buffering is an important part of the QuEChERS procedure because it allows for analysis of pesticides and other residues that display pH dependence in their recoveries, depending on the sample matrix.<h5>Chemicals used in the EN 15662 method</h5><ul><li>Acetonitrile: Used to perform single-phase extraction on a sample</li><li>Anhydrous MgSO₄: Induces phase separation between water content in sample and acetonitrile layer during liquid-liquid partitioning step; removes remaining water from sample during dispersive solid phase extraction (dSPE) step</li><li>NaCl: Induces phase separation between water content in sample and acetonitrile layer during liquid-liquid partitioning step; removes residual water from sample</li><li>Trisodium Citrate: Buffers the sample to stabilize pH</li><li>Disodium Citrate Sesquihydrate: Buffers the sample to stabilize pH</li><li>PSA: Primary secondary amine, used to remove polar matrix components, citric co-extractives, and free fatty acids, from sample during dispersive solid phase extraction (dSPE) step</li><li>C18: Removes fats, sterols, and other non-polar interferences from sample</li><li>GCB: Graphitized carbon black, removes pigment (not recommended for use with planar pesticides)</li></ul><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Thermo_Scientific_Qu_E_Ch_ERS_Sample_Preparation_Kits_3_721562526f.jpg" alt="Thermo Scientific QuEChERS Sample Preparation Kits" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Thermo_Scientific_Qu_E_Ch_ERS_Sample_Preparation_Kits_3_721562526f.jpg 245w," sizes="100vw" width="1471" height="488"><div><h4>QuEChERS clean-up kit for NY/T 1380-2007 method</h4></div><div>The NY/T 1380-2007 method is recommended by China Ministry of Agriculture for the QuEChERS Method sample preparation of 51 specific pesticide residues in fruits and vegetables with analysis by GC-MS.<h5>Chemicals used in the NY/T 1380-2007 method</h5><ul><li>Anhydrous MgSO₄: Induces phase separation between water content in sample and acetonitrile layer during liquid-liquid partitioning step; removes remaining water from sample during dispersive solid phase extraction (dSPE) step</li><li>PSA: Primary secondary amine, used to remove polar matrix components, citric co-extractives, and free fatty acids, from sample during dSPE step</li><li>C18: Removes fats, sterols, and other non-polar interferences from sample</li></ul></div></div></div></div></div></div></div></div></div></div></div></div>

Thermo Scientific Hypersil GOLD C18 Selectivity HPLC Columns

Achieve exceptional peak shape and resolution for your HPLC and LC/MS applications with Thermo Scientific™ Hypersil GOLD™ HPLC columns. 

Achieve exceptional peak shape and resolution for your HPLC and LC/MS applications with Thermo Scientific™ Hypersil GOLD™ HPLC columns. These endcapped, ultrapure, silica-based columns deliver significant reduction in peak tailing using generic gradients with C18 selectivity. With their excellent resolution, efficiency, and sensitivity, Hypersil GOLD columns give you confidence in the accuracy and quality of your analytical data.<h5>Novel Bonding</h5><ul><li>Proprietary derivatization and endcapping</li><li>C18 (USP L1) selectivity</li></ul><h5>pH Stability</h5><ul><li>Robust performance at high pH</li><li>Excellent column stability at low pH</li></ul><h5>Flexible Formats</h5><ul><li>Javelin HTS columns for high-throughput screening</li><li>Semi-preparative columns with 12 μm particles available</li></ul><h4>About the Hypersil GOLD Family of HPLC Columns</h4>Meet the challenges of modern chromatography with state-of-the-art columns that provide outstanding performance and consistent separations. Twelve different column chemistries are available so you can optimize separations and maximize productivity. Get outstanding peak shapes in reversed phase, ion exchange, HILIC, or normal phase chromatography.<ul><li>Excellent peak symmetry</li><li>Narrow peaks for outstanding efficiency</li><li>Enhanced sensitivity and improved resolution</li><li>Exceptional reproducibility for reliable chromatography, column after column</li><li>Solutions for high-throughput screening</li><li>1.9, 3, and 5 μm particle sizes for all your separation needs</li></ul><h4>Specifications</h4><ul><li>Endcapped: Yes</li><li>For Use With: LC/MS</li><li>Max. Pressure: 5800 psi (400 bar)</li><li>Packing Material: Spherical, Fully Porous, Ultrapure Silica</li><li>Stationary Phase: C18 Selectivity</li><li>Surface Area: 220 m²/g</li><li>Temperature: 60°C</li><li>USP Type: L1</li><li>Carbon Load: 11%</li><li>Phase: Reversed Phase</li><li>Pore Size: 175 Å</li><li>pH: 1 to 11</li></ul>

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