<p><strong>He is one of only ten scientists working in Europe who secured the prestigious EMBO Installation Grant this year. After four years in the United States, immunologist Matouš Vobořil recently returned to the Faculty of Science at Charles University to build his own team and advance research focused on the development of immune cells and their training in the thymus. His efforts to combat autoimmune diseases have already caught the attention of numerous grant review panels.</strong></p><h5><strong>Reportedly, one of the motivations for your studies was the awareness that in science, there is still more that is unknown than what is known. You made this statement after receiving the Doctorandus Award for Natural Sciences in 2020, adding that during your lifetime you would like to shift something toward the “known” category. How has that been going so far?</strong></h5><p>The topic for which I&nbsp;take the greatest credit to date is one I&nbsp;began exploring during my doctoral studies and fully developed as a&nbsp;postdoctoral fellow in the U.S., and that is inflammation of the thymus. When you delve into the literature and articles focused on autoimmune diseases, eventually you begin to notice that autoimmune diseases often develop after undergoing some kind of infection. This is particularly evident in connection with long COVID. During inflammatory processes in the body, the immune system responds, and we were interested in whether there is a&nbsp;mechanism that recognises this inflammatory response and teaches T-lymphocytes (immune cells) that this is not a&nbsp;problem, but rather a&nbsp;process inherent to the body.</p><p>During my doctoral studies, I&nbsp;investigated the basic inflammatory response in the thymus, without the presence of actual bacterial or viral inflammation. This turned out to be extremely important. Over time, we discovered that the thymus contains many antiviral molecules and molecules that play a&nbsp;key role in allergic reactions or in the recognition of bacteria and viruses. These molecules create a&nbsp;specific environment in the thymus that mimics what may happen in our bodies later in life, and they train T-cells not to respond to these stimuli. I&nbsp;consider this to be my greatest contribution to the field. Of course, there are still many unexplored areas in this field.</p><h5><strong>The interview I was referring to was published in the magazine of the grammar school where you studied, and which proudly acknowledges your scientific achievements. Was that where your interest in science first began to show?</strong></h5><p>The fact that I&nbsp;studied in Dvůr Králové nad Labem was a&nbsp;huge stroke of luck. Not only is there a&nbsp;zoo, where my parents used to work, so I&nbsp;spent a&nbsp;lot of time there, but thanks to that, I&nbsp;also joined the local Natura club, which brought together people interested in nature and was led by the Dobroruk couple. When I&nbsp;was ten, my parents sent me to a&nbsp;nature-oriented summer camp with them, and I&nbsp;ended up staying with them – which is why I&nbsp;also attended the local high school, where Mrs. Dobroruková taught. She was a&nbsp;wonderful teacher. Her classes really encouraged students to think about what’s&nbsp;happening in nature. Thanks to that, I&nbsp;began participating in biology competitions and got involved in the Czech high school research competition. Although my first scientific experiments focused on ornithology, they gave me the opportunity to present scientific data and meet many people from the Faculty of Science at Charles University, including Professor Jan Černý, who lectured our class on immunology. Suddenly, while still in high school, I&nbsp;realized that this was the path I&nbsp;wanted to take.</p><h5><strong>During your master’s studies, you joined the team of immunologist Dominik Filipp at the Institute of Molecular Genetics of the Czech Academy of Sciences, where you then continued as a doctoral student. How did working with him influence you, and where did it lead you?</strong></h5><p>Dominik Filipp had several ongoing projects related to the peripheral immune response, signalling, and identification of viruses and bacteria. The project I worked on focused on a rare population of cells found in lymph nodes or the spleen. I studied the effect of pathogen-recognising receptors on these cells. Since these cells are similar to those in the thymus and I needed a negative control, I used the thymus for that purpose. To our surprise, the result was not negative but positive. This led us to the phenomenon itself and prompted us to begin investigating its consequences in the thymus.</p><h5><strong>You then travelled overseas to study this discovery – which you actually made rather by chance – in detail. What was your experience like at American research institutions?</strong></h5><p>Amazing. I&nbsp;went to the University of Minnesota to work with particular people, especially my future supervisor, Kristin Hogquist. I&nbsp;had met her at several conferences while I&nbsp;was still in my PhD programme, and I&nbsp;had a&nbsp;feeling that our collaboration could work well both professionally and personally.</p><p>But we were also fortunate to pick Minnesota. It’s&nbsp;a&nbsp;great place to live, and at the same time, excellent science is being conducted there. The Center for Immunology, where I&nbsp;worked, rivals the world’s&nbsp;leading scientific institutions in terms of its research results. I&nbsp;think this is mainly down to the highly supportive environment there, which fosters collaboration. Thanks to this, you can really grow as a&nbsp;scientist there.</p><p>In addition, they focus on explaining and describing the chosen phenomenon as a&nbsp;whole, rather than worrying too much about whether it will result in one or five articles, or whether it will take ten years. Their method of funding supports this approach. In a&nbsp;system of short-term grants, as we know it here, you can’t structure research this way. That’s&nbsp;a&nbsp;major disadvantage for us.</p><h5><strong>How did you develop your own research there?</strong></h5><p>It was there that I&nbsp;fully realized the potential of what we had discovered in the lab in Prague. Kristin Hogquist and her team were working on something similar, but it wasn’t until we teamed up that we were able to take it further. I&nbsp;focused primarily on how various antiviral signals or signals triggered during allergic reactions influence T-cell training and defence against autoimmune diseases following viral infections or allergic reactions. Suddenly, it became clear that this was a&nbsp;vast topic and the foundation for establishing a&nbsp;separate subfield of research on tolerance (the training of immune cells in the thymus so that they do not harm the body while remaining capable of fighting pathogens).</p><p>One great thing was that two labs were coming together at the facility: the one led by Kristin Hogquist and the other led by her husband, Stephen Jameson, which focuses on how different types of infections affect T-cell activation and their response. I&nbsp;realised that these two areas needed to be connected, and I&nbsp;based my own research plan on that – namely, to study how the inflammatory environment, actual inflammation, and viral or bacterial infections can retroactively influence processes in the thymus. This is a&nbsp;topic that no one has addressed yet.</p><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Forum_UK_Voboril_O_imunitnim_systemu_toho_stale_mnoho_nevime_2_b020119f2e.jpg" alt="Forum UK/Petr Jan Juračka: Vobořil: There is still much to learn about the immune system: As head of research, Matouš Vobořil strives to ensure that everyone on his team feels comfortable." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Forum_UK_Voboril_O_imunitnim_systemu_toho_stale_mnoho_nevime_2_b020119f2e.jpg 234w," sizes="100vw" width="1000" height="667"></p><h5><strong>You mentioned how important it was to you that you got along with Kristin Hogquist not only professionally but also on a&nbsp;personal level. To what extent did you take this “personal” aspect into account when putting together your own team at CU SCI?</strong></h5><p>This is tremendously important to me. Kristin Hogquist is not only an outstanding researcher, but also a&nbsp;very kind person. And that really helped me feel at home in the American environment, which sometimes tends to become highly competitive. I&nbsp;realized that I&nbsp;would like those who will one day work on my own team to feel the same way I&nbsp;did on hers.</p><h5><strong>So far, we’ve been talking about what led you to science. Have you, on the other hand, encountered anything during your career so far that has discouraged you from pursuing science?</strong></h5><p>Moving overseas with my family and my two-and-a-half-year-old son during the pandemic wasn’t the easiest thing to do. We had to apply for a&nbsp;national exemption just so that I&nbsp;and my family would be allowed into the U.S. at all. Fortunately, as an immunologist, I&nbsp;fell into a&nbsp;special category subject to less stringent pandemic restrictions. Packing up the family, moving overseas, and learning to navigate a&nbsp;completely different system is undoubtedly a&nbsp;major challenge. It required sacrifices not only from me, but above all from my wife, who had to put her own career on hold for several years because of mine. This is one of the biggest and most demanding obstacles that scientists and their families must overcome.</p><p>The second major obstacle you have to deal with as a&nbsp;scientist is paperwork – something I’ve only fully realized now that I’m leading a&nbsp;research group. Even though you hear about it from those around you, it still doesn’t prepare you for when it comes. As a&nbsp;trainee, you do science and conduct experiments in the lab, but suddenly everything shifts, and you become a&nbsp;manager and administrator of all sorts of things. I&nbsp;spend about 30 percent of my time on paperwork. It’s&nbsp;not exactly fun.</p><h5><strong>You obtained a&nbsp;Primus grant, a&nbsp;Junior Star grant from the Czech Science Foundation, and most recently an EMBO Installation Grant. What research challenges do you now face with these grants?</strong></h5><p>I&nbsp;would also like to apply for an ERC Starting Grant this year as well. In the Junior Star project, we are studying cell migration to the thymus. We hypothesize that so-called dendritic cells migrate to the thymus from the periphery (skin, lungs, intestines) – that is, from sites exposed to the external environment and inflammation. These cells are thought to bring information about their own antigens to the thymus and teach developing T-cells tolerance. We aim to determine whether this migration actually occurs, under what circumstances, and how important it is for establishing tolerance to specific tissues. This could help answer the question of why autoimmune skin diseases, such as psoriasis, develop following inflammatory processes.</p><p>The second objective is broader. We would like to answer a&nbsp;major question in immunology: <strong>why do autoimmune diseases develop after a&nbsp;person has had an infection? </strong>We want to study how different types of inflammation (viral, bacterial, or yeast infections) affect the training of immune cells in the thymus.</p><p>We would like to introduce a&nbsp;system of so-called “dirty mice.” The entire field of immunological research is based on working with mice raised in sterile conditions so that we can easily manipulate them and observe how these manipulations affect the immune system. In reality, however, the immune system does not function this way. I&nbsp;brought this idea back from the University of Minnesota, where they placed standardized mice in the same cage with mice purchased from various pet stores around Minneapolis. After a&nbsp;few weeks to months, the mice contract almost everything the store-bought mice have, thereby developing a&nbsp;true physiological immune response. Yet they are still the same mice – genetically identical – that we can manipulate. This is an excellent system that allows us to study immune responses in a&nbsp;much more physiological context.</p><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Forum_UK_Voboril_O_imunitnim_systemu_toho_stale_mnoho_nevime_3_c458457e12.jpg" alt="Forum UK/Petr Jan Juračka: Vobořil: There is still much to learn about the immune system " srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Forum_UK_Voboril_O_imunitnim_systemu_toho_stale_mnoho_nevime_3_c458457e12.jpg 234w," sizes="100vw" width="1000" height="667"></p><h5><strong>Will you be applying for a&nbsp;European Research Council (ERC) grant with that goal in mind?</strong></h5><p>Exactly.</p><h5><strong>In order to succeed in the competition for ERC grants, the projects should push the boundaries of knowledge, which also involves a&nbsp;certain degree of risk. Where do you see the greatest risks in your own research?</strong></h5><p>There are many risks. One of the biggest is that although all the infections we plan to introduce are controlled and well-documented, you still never have full control over them. So it’s&nbsp;possible that not only will the mice become peripherally infected, but the thymus itself will also become infected, which could complicate the experiments. At the same time, this is a&nbsp;physiological system – and I’m talking mainly about the “dirty mice” – which means that each mouse will respond a&nbsp;little differently, and that will generate enormous variability in the samples. Immunologists strive to have the purest system possible, so that when they introduce, for example, a&nbsp;single infectious agent, they can then observe exactly what’s&nbsp;happening there. In this regard, we are actually taking a&nbsp;step back, so to speak, because we want it to be more variable and closer to reality. Setting up and analysing data from such experiments so that they’re reproducible and reveal a&nbsp;real phenomenon can be a&nbsp;real challenge. But that’s&nbsp;the only way we can get closer to real life.</p><h5><strong>The most recent Nobel Prize in Physiology or Medicine was awarded to three immunologists: Mary E. Brunkow, Fred Ramsdell, and Shimon Sakaguchi, who recently gave a&nbsp;lecture in Prague. Did you meet him? How much did awarding the Nobel Prize to immunologists motivate you further in your work?</strong></h5><p>When Shimon Sakaguchi was in Prague, I&nbsp;was still in the U.S., but I&nbsp;did meet him several times at various conferences and even had the opportunity to talk with him on multiple occasions, given that we’re both involved in T-cell research. In our community, people have long been placing bets on when they’ll finally get the Nobel Prize. For me, their discoveries are truly inspiring, but the Nobel Prize itself doesn’t motivate me that much anymore. I&nbsp;find the story behind it to be more interesting. It took a&nbsp;very long time for the scientific community to accept their findings. One of the seminal articles was published in the <i>Journal of Immunology</i>, which isn’t a&nbsp;journal with as high a&nbsp;profile as <i>Nature</i> or <i>Science,</i> and not one where you’d expect to read about such a&nbsp;groundbreaking discovery. It took several years before it became clear just how significant this discovery was. Now I&nbsp;often remind my students of this – even an article in the <i>Journal of Immunology</i> can win you a&nbsp;Nobel Prize, and that is incredibly inspiring.</p><blockquote><h5><strong>Matouš Vobořil, PhD</strong></h5><p>Graduate of the Faculty of Science at Charles University. His research focuses on the development of immune cells in the thymus, the onset of autoimmune diseases, and the function of antigen-presenting cells. After spending four years as a&nbsp;postdoctoral fellow at the University of Minnesota on Kristin Hogquist’s&nbsp;team, he returned to Europe in 2025. Thanks to a&nbsp;Primus grant from Charles University, he established his own research group at the Faculty of Science. He subsequently received a&nbsp;Junior Star grant from the Czech Science Foundation and, this January, an EMBO Installation Grant. For his dissertation, he was awarded the Doctorandus Prize as part of the Česká hlava initiative.</p></blockquote>

Vobořil: There is still much to learn about the immune system 

<h3><strong>How are PFAS regulations changing, and what role will ion chromatography play in the future of environmental testing?</strong></h3><p>In this episode, &nbsp;David interviews <strong>Jay Gandhi, PhD, </strong>Vertical Markets Manager at <strong>Metrohm USA</strong>, for an in-depth discussion on PFAS analysis, the rise of AOF (Adsorbable Organic Fluorine) testing, and the development of EPA 1621 and ISO 18127 standards.</p><p>With more than 30 years of experience in ion chromatography, Jay shares valuable perspective on how the field has evolved—from early applications and NASA water recovery projects to today’s challenges involving PFAS, TFA, and emerging contaminants.</p><h4><strong>Topics covered in this episode include:</strong></h4><ul><li>What PFAS are and why they matter</li><li>The difference between targeted PFAS methods and AOF analysis</li><li>How combustion ion chromatography works</li><li>Behind the scenes of EPA 1621 and ISO 18127 method development</li><li>Regulatory trends in water, textiles, and food packaging</li><li>TFA analysis using IC-MS and IC-MS/MS</li><li>The future of ion chromatography in environmental labs</li></ul><h3><strong>Video Transcription</strong></h3><p>For more than three decades, Jay Gandhi has worked with ion chromatography (IC), watching the technique expand from a specialized tool for water analysis into an analytical platform used across environmental testing, food and beverage analysis, semiconductor manufacturing, battery applications, and, increasingly, PFAS analysis.</p><p>In an interview for <i>Concentrating on Chromatography</i>, Gandhi discusses his introduction to IC during his work on a NASA Mars exploration project, the emergence of PFAS as a major analytical challenge, and why techniques such as adsorbable organic fluorine (AOF) determination and combustion ion chromatography (CIC) can provide information that targeted LC-MS analysis alone cannot deliver. He also examines the development of standardized methods, sample preparation challenges, and the future role of IC-MS and total fluorine approaches in PFAS monitoring.</p><h4><strong>From NASA to ion chromatography</strong></h4><p>Gandhi's introduction to ion chromatography came through his work as a principal scientist on a NASA Mars exploration project focused on water recovery under zero-gravity conditions. IC played an important role because it enabled researchers to establish the ionic balance of anions and cations in water.</p><p>The technology was used both to investigate micronutrients relevant to agricultural applications and to evaluate water purity for potential astronaut consumption. During approximately three years of this work, Gandhi gained extensive experience with ion chromatography. This ultimately led him deeper into IC instrumentation and, 24 years ago, to joining Metrohm.</p><p>At the beginning of his career, IC was strongly associated with relatively straightforward anion and cation analysis in water. The technique itself had been used for water analysis since the 1970s, but Gandhi has seen its application range expand dramatically.</p><p>Food and beverage testing and environmental analysis became important fields, supported by both direct and indirect detection strategies. More recently, IC has also found applications in areas such as the semiconductor industry and battery recycling.</p><p>In Gandhi's words, ion chromatography has effectively "bloomed" into numerous application areas.</p><h4><strong>PFAS changes the analytical challenge</strong></h4><p>PFAS represent a particularly important chapter in this development. These chemicals have been used for decades in numerous consumer and industrial products, including non-stick materials, firefighting foams, water-resistant textiles and other applications.</p><p>Gandhi recalls work on PFOA and PFOS using ion chromatography with conductivity detection as early as 2008. However, the achievable detection limits were insufficient for the extremely low concentrations that later became important in PFAS analysis.</p><p>A major turning point for him came in 2015, when he encountered research from Sweden using <strong>combustion ion chromatography to investigate total fluorine</strong>. Subsequent collaboration with researchers at Clarkson University and Harvard University, particularly on aqueous film-forming foam (AFFF), further demonstrated the importance of looking beyond individual targeted PFAS compounds.</p><h4><strong>Why targeted LC-MS does not tell the whole story</strong></h4><p>Targeted LC-MS is highly sensitive and selective and can provide detailed information about individual PFAS compounds. However, according to Gandhi, the number of possible PFAS structures in the environment is vastly greater than the number routinely included in targeted methods.</p><p>A targeted method may monitor dozens or perhaps around one hundred compounds, while the overall PFAS universe can include thousands of chemical structures. Consequently, targeted analysis may characterize only a small fraction of the total PFAS burden.</p><p>There are other practical considerations. LC-MS measurements can be affected by matrix-dependent ion suppression, while accurate quantification frequently relies on isotope-labelled internal standards. Such standards are available for only a limited number of compounds and can be expensive.</p><p>This is where measurements of total or adsorbable organic fluorine can provide complementary information. Rather than asking <i>which specific PFAS compounds are present?</i>, these approaches can help answer a broader question: <i>how much fluorine associated with this chemical space is present in the sample?</i></p><h4><strong>AOF as a complementary approach</strong></h4><p>Adsorbable organic fluorine analysis provides a different perspective from targeted LC-MS. In the workflow discussed by Gandhi, organic fluorine compounds are captured during sample preparation and subsequently measured using combustion ion chromatography.</p><p>The principle of CIC builds upon older total organic halogen analysis. The sample is combusted at temperatures above approximately 1,000 °C, breaking carbon-halogen bonds. In PFAS-related analysis, carbon-fluorine bonds are broken and the resulting fluoride is collected in an absorption solution.</p><p>Ion chromatography is then used to separate and quantify the fluoride.</p><p>Replacing older detection approaches with IC provides both sensitivity and selectivity for the halogens being measured. Effective combustion is critical: oxygen supports the combustion process, while a carrier gas transports the products into the absorption solution for subsequent IC analysis.</p><h4><strong>The challenge of inorganic fluoride</strong></h4><p>The broad response of CIC is simultaneously one of its strengths and one of its analytical challenges. Combustion does not distinguish fluorine originating from PFAS from fluorine present in inorganic compounds such as fluoride salts.</p><p>This becomes particularly important when analyzing water containing relatively high concentrations of inorganic fluoride while attempting to quantify organic fluorine at very low levels.</p><p>Sample preparation therefore has a critical role. Inorganic fluoride must be effectively separated from the fraction of interest, while materials used during sample preparation must themselves have sufficiently low fluoride backgrounds.</p><p>Contamination can come from unexpected sources. Gandhi points out that background fluoride may originate not only from reagents and sample preparation materials but potentially from the laboratory environment. Careful blank control, appropriate materials and rigorous evaluation of interferences are therefore essential when moving toward increasingly low detection limits.</p><h4><strong>From research method to international standardization</strong></h4><p>The development of standardized AOF methods illustrates the importance of collaboration between academia, industry, regulatory organizations and analytical laboratories.</p><p>Gandhi describes work that began in Europe around 2015 and subsequently progressed through German standardization activities, ISO development, ASTM-related collaboration and the U.S. EPA. The process eventually contributed to methods such as <strong>EPA Method 1621</strong>.</p><p>He emphasizes that this was not the work of a single scientist or organization. Developing a robust method required years of experiments, discussions and sometimes difficult technical debates among numerous stakeholders.</p><p>For Gandhi, the essential principle was that experimental evidence ultimately had to determine the outcome. Collaboration becomes possible when scientists focus on the data rather than the organizations they represent.</p><h4><strong>PFAS monitoring is moving beyond drinking water</strong></h4><p>Regulatory attention to PFAS is also expanding beyond drinking water. Gandhi describes a broader strategy that begins with reducing the manufacture and use of PFAS-containing materials and continues with removing existing contamination from the environment.</p><p>Wastewater represents an important part of this picture because PFAS can enter it from numerous sources. Historical contamination from landfills and contaminated sites can also migrate into groundwater and ultimately affect drinking-water supplies.</p><p>Consumer products are another area of increasing attention. Food packaging, disposable containers, automotive fabrics, outdoor clothing and water-repellent textiles have historically used fluorinated substances to provide properties such as grease, water or stain resistance.</p><p>Reducing PFAS use in such products could therefore gradually decrease the amount entering waste streams and the wider environment.</p><h4><strong>Screening first, regulation later?</strong></h4><p>Gandhi expects AOF to be particularly valuable as a <strong>screening tool</strong>, providing an estimate of broader fluorine contamination before laboratories proceed to more specific analyses.</p><p>Its importance becomes clear when AOF results are compared with targeted PFAS measurements. Gandhi reports that laboratories working with EPA Method 1621 have observed differences between targeted PFAS results and broader AOF measurements ranging from approximately <strong>300- to 4,000-fold in the total fluorine mass balance</strong>.</p><p>Such discrepancies suggest that targeted methods can leave a substantial portion of fluorine unexplained.</p><p>Rather than competing with LC-MS, AOF and CIC therefore provide complementary information. A broad screening method can reveal the magnitude of total contamination, while targeted mass spectrometry identifies and quantifies specific compounds of interest.</p><h4><strong>Where IC-MS fits into the PFAS toolbox</strong></h4><p>No single technique can cover the entire PFAS problem. Gandhi highlights very short-chain, highly polar fluorinated compounds as another analytical challenge.</p><p>Compounds such as trifluoroacetic acid (TFA) are highly water-soluble and may not be quantitatively retained by the carbon materials used for AOF sample preparation. Direct ion chromatography can provide an alternative route for separating such compounds from common inorganic anions.</p><p>Coupling IC with mass spectrometry adds another level of sensitivity and selectivity. Gandhi therefore sees <strong>IC-MS and IC-MS/MS</strong> as increasingly important tools for highly polar fluorinated compounds. Similar approaches can also be useful beyond PFAS, including the determination of polar pesticides such as glyphosate and glufosinate.</p><h4><strong>Toward lower detection limits and more complete PFAS analysis</strong></h4><p>For Gandhi, current standardized methods represent a beginning rather than an endpoint. Improvements in instrumentation and particularly sample preparation could push total fluorine analysis to substantially lower concentrations.</p><p>He notes that current combustion IC configurations can reach approximately <strong>0.5 ppb</strong>, or 500 parts per trillion, and believes that improvements in sample volume, preparation procedures and mitigation of inorganic fluoride backgrounds could eventually move detection below 100 ppt and potentially toward 50 ppt.</p><p>Extractable organic fluorine workflows could provide another bridge between broad fluorine screening and targeted LC-MS methods. Combining improved extraction with CIC could allow samples to be screened for organic fluorine before more detailed compound-specific characterization.</p><p>The broader message from Gandhi's career is that PFAS analysis is unlikely to be solved by a single analytical technique. <strong>Targeted LC-MS provides compound-specific sensitivity and selectivity; AOF and combustion IC reveal a broader fluorine picture; and IC-MS can address highly polar compounds that are difficult to capture using conventional workflows.</strong> Used together, these techniques can provide laboratories with a much more complete understanding of PFAS contamination than any one approach alone.</p><blockquote><p><strong>This text has been automatically transcribed from a video presentation using AI technology. It may contain inaccuracies and is not guaranteed to be 100% correct.</strong></p></blockquote><blockquote><h4><strong>Concentrating on Chromatography Podcast</strong></h4><p>Dive into the frontiers of chromatography, mass spectrometry, and sample preparation with host David Oliva. Each episode features candid conversations with leading researchers, industry innovators, and passionate scientists who are shaping the future of analytical chemistry. From decoding PFAS detection challenges to exploring the latest in AI-assisted liquid chromatography, this show uncovers practical workflows, sustainability breakthroughs, and the real-world impact of separation science. Whether you’re a chromatographer, lab professional, or researcher you'll discover inspiring content!</p><p>You can find <strong>Concentrating on Chromatography Podcast </strong>in podcast apps:</p><ul><li><a target="_blank" rel="noopener noreferrer" href="https://open.spotify.com/show/1wZg67rCjoGju4yhqxI3tL"><strong>Spotify</strong></a></li><li><a target="_blank" rel="noopener noreferrer" href="https://podcasts.apple.com/tz/podcast/concentrating-on-chromatography/id1788429185"><strong>Apple podcast</strong></a></li><li><a target="_blank" rel="noopener noreferrer" href="https://podscan.fm/podcasts/concentrating-on-chromatography"><strong>podscan</strong></a></li></ul><p>and on <a target="_blank" rel="noopener noreferrer" href="https://www.youtube.com/@ChromatographyTalk/videos"><strong>YouTube channel</strong></a></p></blockquote>

The Future of Ion Chromatography | PFAS Testing, EPA 1621 & AOF Explained

<p><strong>In late February 2025, a train carrying benzene derailed in Hustopeče nad Bečvou, and within hours, a race was on to save the groundwater and surface water. One of the most toxic industrial substances began seeping into the gravel-sand subsoil. While fieldwork was underway to prevent the spread of contamination, decisions were already being made in the CXI TUL laboratories regarding how to subsequently clean up the contaminated rock environment.</strong></p><p>The accident ranks among the <strong>most serious environmental incidents in the Czech Republic in recent years</strong>. After a tanker train derailed, hundreds of<strong> metric tons of benzene leaked</strong>; due to the highly permeable geological subsoil, it quickly seeped into deeper soil layers and into the groundwater. The combination of the substance’s high toxicity and local conditions complicated both the emergency response and the subsequent remediation. The key was to stop further spread, determine the extent of the contamination, and establish a remediation strategy for the entire site.</p><p>It was at this stage that CXI TUL became involved in the solution. At the request of the renowned remediation company DEKONTA, a.s., a team led by <strong>RNDr. Jan Němeček, Ph.D.</strong>, began testing the feasibility of the<strong> in situ chemical oxidation (ISCO) method</strong>. This technology is designed to<strong> break down benzene directly within the rock matrix</strong>, without the need for extensive excavation of contaminated soil or the pumping out of large volumes of groundwater. The goal was simple: to find a method that would work quickly, safely, and under the actual conditions of the affected site.</p><h4><strong>Benzene Remediation: Technically Simple, Complicated in Practice</strong></h4><p>Benzene is a highly toxic and carcinogenic substance that rapidly penetrates into groundwater in permeable subsoil, where it dissolves and spreads further as the water flows toward surface water bodies. Benzene also binds to rock particles and migrates in the form of vapors into overlying unconsolidated layers.</p><p>Scientists in Liberec therefore tested <i>in situ </i>chemical oxidation (ISCO) technology under laboratory conditions. The goal was to find an oxidant capable of breaking down benzene directly underground into less hazardous substances.</p><p>The tests evaluated two main options:<strong> activated hydrogen peroxide and sodium peroxodisulfate</strong>. Both substances removed up to<strong> 99.99% of the benzene</strong>, but there was a difference in their kinetics and impacts on the natural environment. Activated hydrogen peroxide achieved nearly complete degradation of the contaminant within a single day. Activated sodium peroxodisulfate was slower and also significantly altered the chemistry of the water; it acidified the environment and increased the sulfate content in the water, which poses an additional environmental burden.</p><p>For field applications, the researchers therefore unequivocally recommended <strong>activated hydrogen peroxide</strong>.</p><h4><strong>Dosage Matters</strong></h4><p>It was not just the oxidant’s effectiveness that was key. It was equally important to determine how the chemical reaction would behave in a real geological environment. The team of scientists therefore also analyzed what is known as <strong>soil oxidant consumption (SOD)</strong>. It turned out that a significant portion of the oxidizing agent does not react solely with benzene but is also consumed to a significant extent by the rock environment itself, particularly by natural organic substances present in the fine-grained fraction of the soil.</p><p>An oxidant consumption value was determined specifically for the local composition of the aquifers, which is essential for the correct dosing of chemicals in the field so that the remediation is not only effective but also economically viable and safe for surrounding ecosystems.</p><h4><strong>One Year After the Accident: Research Turns into Real-World Assistance</strong></h4><p>While the public often perceives university science as a distant world of laboratories and grant projects, it is precisely situations like this that demonstrate its practical impact. The results of the work by researchers at CXI TUL did not remain confined to academic studies but directly influenced decisions regarding the technology used to clean up one of the largest environmental accidents in recent years.</p>

Science on the Front Lines: Cleanup of a Benzene Spill

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Thermo Scientific Stellar Mass Spectrometer

Move biomarker candidates from discovery to validation more efficiently, confidently, and at lower cost with breakthroughs in extended scale, massive throughput, higher sensitivity, greater specificity, and unrivaled productivity.

<div><div id="container-a1c36dfacf"><div><div><div id="text-913638046"><p><strong>A new paradigm for targeted quantitation.&nbsp;Move biomarker candidates from discovery to validation more efficiently, confidently, and at lower cost with breakthroughs in extended scale, massive throughput, higher sensitivity, greater specificity, and unrivaled productivity.</strong></p><p>The Thermo Scientific Stellar Mass Spectrometer introduces revolutionary technology combining robust quantitation with the specificity of rapid full scan MS<sup>n</sup>&nbsp;acquisition creating unrivaled improvements in laboratory productivity.</p><div><div id="text-801893275"><h3><strong>Expanded experimental capabilities to meet the most challenging biomarker verification</strong></h3></div></div><div><div id="container-1e8e478f3a"><div><div><div id="text-1204752335"><p>The Thermo Scientific Stellar Mass Spectrometer offers an array of data acquisition capabilities to deliver robust and reproducible quantitation for a wide range of analytes across an extended dynamic range.</p></div></div></div></div></div><h4><strong>System highlights</strong></h4><div><div id="container-a89a56f063"><div><div><div id="text-451891225"><h5><strong>Improve ionization efficiency for more compounds at higher LC flow rates&nbsp;</strong></h5></div></div><div><div id="text-1671602085"><p>For a wider range of compounds separated using liquid chromatography operated at analytical flow rates, the Stellar Mass Spectrometer comes equipped with the Thermo Scientific OptaMax Plus Ion Source, which can deliver higher vaporizer temperatures to improve desolvation and ionization potentials.</p></div></div></div></div></div><figure class="image"><img style="aspect-ratio:1290/725;" src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Pragolab_Thermo_Scientific_Stellar_revolucni_hmotnostni_spektrometr_SF_Sys_Opta_Max_c303cf500a.jpg" alt="Thermo Fisher Scientific: Thermo Scientific Stellar Mass Spectrometer - SF Sys OptaMax" width="1290" height="725"></figure><div><div id="container-d79c3b2946"><div><div><div id="text-251514658"><h5><strong>Spend less time on instrument set-up and focus on the science</strong></h5></div></div><div><div id="text-989104128"><p>Trigger on-demand or scheduled system calibrations remotely and without disruption to your LC-MS system with the integrated Auto-Ready ion source.</p></div></div></div></div></div><div><div id="container-81dd07c9bb"><div><div><div id="text-1289282711"><h5><strong>Improve ion utilization by extending the capabilities of the new collision cell</strong></h5></div></div><div><div id="text-1641484825"><p>The ion concentrating routing multipole (ICRM) is a curved collision cell performing both beam-type fragmentation (HCD) and precursor/product ion accumulation capabilities as controlled by dynamic automatic gain control (AGC) with synchronized ion packet release to the high-pressure linear ion trap for downstream manipulation or detection.</p></div></div></div></div></div><figure class="image"><img style="aspect-ratio:1290/725;" src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Pragolab_Thermo_Scientific_Stellar_revolucni_hmotnostni_spektrometr_SF_Sys_ICRM_3b2326f3af.jpg" alt="Thermo Fisher Scientific: Thermo Scientific Stellar Mass Spectrometer - SF Sys ICRM" width="1290" height="725"></figure><div><div id="container-dbf5a6cdd9"><div><div><div id="text-813962034"><h5><strong>Efficiently manage ion populations for fast and sensitive analysis</strong></h5></div></div><div><div id="text-2033771024"><p>The&nbsp;hyper-fast dual-pressure linear ion trap mass analyzer expands analytical capabilities while maximizing fast, high-quality data acquisition.</p><p>The differential neutral pressures maintained in the high- and low-pressure linear ion traps prepares ions for higher-order manipulation or fast and sensitive ion ejection at scan rates up to 220 kDa/S, but typically operates at 125 kDa/S for robust data acquisition.</p></div></div></div></div></div><div><div id="container-9a523f34e0"><div><div><div id="text-1491120095"><h5><strong>Extended analytical capabilities to increase laboratory productivity</strong></h5></div></div><div><div id="text-328051555"><p>The high-pressure linear ion trap maximizes ion packet manipulation enabling multi-notch RF excitation for dissociation or ejection for SIM, higher order MS<sup>n</sup>, SPS MS<sup>3</sup>, or data-independent acquisition (DIA) to address the most challenging quantitative experiments.&nbsp;</p><div><div id="container-82e5907416"><div><div><div id="text-693866740"><h5><strong>Leverage single ion detection sensitivity to address the most challenging quantitation demands</strong></h5></div></div><div><div id="text-737565098"><p>Efficient and sensitive ion detection drives improved quantitative performance at scale. The dual conversion dynode configuration coupled to the photomultiplier tube detects ca. 84% of trapped ions for single ion sensitivity.</p></div></div></div></div></div></div></div></div></div></div><figure class="image"><img style="aspect-ratio:2048/1152;" src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Pragolab_Thermo_Scientific_Stellar_hmotnostni_spektrometr_SF_Sys_Extended_Scale_750dc20128.jpg" alt="Thermo Fisher Scientific: Thermo Scientific Stellar Mass Spectrometer - SF Sys Extended Scale" width="2048" height="1152"></figure><div><div id="text-1993749933"><h5><strong>Automated intelligence manages tMSn acquisition</strong></h5></div></div><div><div id="text-1206797739"><p>Streamline tMSn method creation and data acquisition management. PRM Conductor software effectively and efficiently creates robust targeted quantitative methods and manages reproducible data acquisition and processing.</p></div></div><div><div id="container-b03b606507"><div><div><div id="text-2005266584"><h5><strong>Maximize data completeness with the Adaptive RT routine</strong></h5></div></div><div><div id="text-671832198"><p>Eliminate the cost and time of optimizing RT kits to the study samples to boost timed/scheduled data acquisition performance using the Adaptive RT routine.</p><p>The method leverages a novel real-time mapping routine to dynamically adjust timed/scheduled data acquisition windows at narrower time intervals to eliminate missing values that compromise data quality.&nbsp;</p></div></div></div></div></div><figure class="image"><img style="aspect-ratio:1290/654;" src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Pragolab_Thermo_Scientific_Stellar_revolucni_hmotnostni_spektrometr_SF_Sys_PRM_Conductor_5b596e7e4c.jpg" alt="Thermo Fisher Scientific: Thermo Scientific Stellar Mass Spectrometer - SF Sys PRM Conductor" width="1290" height="654"></figure><div><div id="container-a192ba4b83"><div><div><div id="container-38d4c699d2"><div><div><div id="text-1578189934"><h5><strong>Industry-leading polarity switching speeds increase laboratory productivity</strong></h5></div></div><div><div id="text-1173595596"><p>Reduce the time, cost, and challenges associated with creating and implementing highly multiplexed quantitative metabolite analysis. Fast polarity switching speeds enable comprehensive compound interrogation in +ESI and -ESI at all MS<sup>n</sup> levels on an LC timescale.</p><div><div id="container-b5fb525888"><div><div><div id="text-18006314"><h5><strong>Environmentally friendly dry pumps decrease energy usage and maintenance</strong></h5></div></div><div><div id="text-1704557304"><p>Changing from two oil-based fore pumps to a single dry pump reduces heat output by 22% and decreases maintenance.</p><p>The use of a single dry pump also eliminates oil vapors and the need for oil checks and changes.</p><h3><strong>Solutions for additional research support</strong></h3><div><h4><strong>Translational proteomics</strong></h4><p>The Stellar Mass Spectrometer offers new possibilities for translational proteomics with improved specificity for greater quantitative performance at scale.</p><h4><strong>Quantitative proteomics</strong></h4><p>Accurate and precise quantitation for label-free and TMT multiplexing sample analysis strategies with the Stellar Mass Spectrometer.</p><div><h4><strong>Metabolomics and Lipidomics</strong></h4><p>Extended analytical capabilities quantify more metabolites and lipids with greater specificity using faster liquid chromatography gradients to increase laboratory productivity.</p></div></div></div></div></div></div></div></div></div></div></div></div></div></div></div></div></div></div></div></div>

Thermo Scientific Orbitrap Exploris MX Mass Detector

Accelerate biopharmaceutical late-stage development, commercialization, manufacturing, and quality control (QC) by easily and reliably obtaining compliance-ready, high-quality mass information. 

<p><strong>Accelerate biopharmaceutical late-stage development, commercialization, manufacturing, and quality control (QC) by easily and reliably obtaining compliance-ready, high-quality mass information.&nbsp;</strong></p><p>The <strong>Thermo Scientific Orbitrap Exploris MX mass detector</strong> is the perfect fit for deploying routine mass monitoring.</p><p>In addition to high-resolution mass detection provided by Thermo Scientific Orbitrap mass analyzers, this fit for purpose system is simple to operate and compliance ready with <a target="_blank" rel="noopener noreferrer" href="https://www.thermofisher.com/cz/en/home/industrial/chromatography/chromatography-learning-center/chromeleon-cds-information/cds-mass-spectrometry-software.html">Thermo Scientific Chromeleon chromatography data system (CDS) software</a>. Consistent performance between the <a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/products/402">Thermo Scientific Orbitrap Exploris 240 mass spectrometer</a> and Orbitrap Exploris MX detector ensures seamless method transfer from development to QC.</p><h4><strong>High-resolution accurate-mass (HRAM) data provide utmost confidence for:</strong></h4><ul><li>Sequence confirmation</li><li>Peptide monitoring</li><li>Intact protein analysis (from small to large molecules, reduced mAbs, and intact mAbs under native and denaturing conditions)</li><li>Glycan profiling</li><li>Oligonucleotide and oligonucleotide impurity mass confirmation</li></ul><h3><strong>Highlights of Orbitrap Exploris MX mass detector&nbsp;</strong></h3><div colctrl-id="5-1"><div><div container-id="cq-colctrl-lt0" comp-id="A1"><div><h4><strong>Simplicity for scientists and technicians</strong></h4><p>Simplify tasks and reduce errors for more ‘right first time’ analyses with Chromeleon CDS, with built in methods, control, data processing, and management. The suite of smart tools makes your work faster and easier, while ensuring reproducible, high-quality mass monitoring.</p></div></div></div><div><div container-id="cq-colctrl-lt0" comp-id="B1"><div><h4><strong>Accelerate productivity</strong></h4><p>High uptime boosts productivity. Rapid “set and forget” calibration procedures provide consistent mass stability for at least four weeks at prescribed conditions. Instrument status monitoring and optimized pressure control alert users when maintenance is needed, avoiding unnecessary downtime and repeat analyses.</p></div></div></div></div><div colctrl-id="6-1"><div><div container-id="cq-colctrl-lt0" comp-id="A1"><div><h4><strong>Compliance for competitive advantage</strong></h4><p>Designed for data integrity, data security, and compliance, fully scalable Chromeleon CDS provides comprehensive technical controls to give you a competitive edge in meeting global regulatory requirements.</p></div></div></div><div><div container-id="cq-colctrl-lt0" comp-id="B1"><div><h4><strong>Consistent results deliver confidence</strong></h4><p>Robust and reliable performance from system to system and site to site ensure confidence in results used to make critical decisions. Industry-proven Orbitrap technology, now purposely designed for mass monitoring in future-proof lab operations.</p></div></div></div></div><div colctrl-id="7-1"><div><div container-id="cq-colctrl-lt0" comp-id="A1"><div><h4><strong>Seamless method transfer to MAM</strong></h4><p>Easy transfer of sophisticated Multi-Attribute Method (MAM) assays from development to manufacturing bridges the gap between development and QC.</p></div></div></div><div><div container-id="cq-colctrl-lt0" comp-id="B1"><div><h4><strong>Dedicated, global support team</strong></h4><p>The dedicated, global Thermo Scientific MAM team maximizes your productivity and confidence with installation, training, and fast service and support. Standardized system performance evaluation test (SET) and installation qualification/operational qualification (IQ/OA) thoroughly assess system performance with comprehensive installation and post-maintenance acceptance criteria.</p><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Thermo_Scientific_Orbitrap_Exploris_MX_Mass_Detector_4_2ad72d9b21.jpg" alt="Orbitrap Exploris MX mass stability: mass deviation relative to first observed mass in parts-per-million for 9 BSA tryptic peptides monitored over a period of 6 weeks using the new internal recalibration option. Each data point is an average of 19 individual injections." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Thermo_Scientific_Orbitrap_Exploris_MX_Mass_Detector_4_2ad72d9b21.jpg 184w," sizes="100vw" width="1240" height="1052"></p><h3><strong>Accelerate MAM deployment&nbsp;</strong></h3><h4><strong>Seamless knowledge and method transfer</strong></h4><p>Benefit from the end-to-end Thermo Scientific MAM 2.0 workflow approach to comprehensive characterization and monitoring of biotherapeutics from development to commercialization. <a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/products/402">Orbitrap Exploris 240</a> mass spectrometer methods, established during development, seamlessly transfer to the Orbitrap Exploris MX detector in manufacturing or by contract partners. In addition to full integration into the high-resolution MAM 2.0 workflow, the usability and low cost of the Orbitrap Exploris MX detector make it the perfect fit for high-throughput QC and contract labs.</p><p>Users can identify and evaluate relevant product quality attributes (PQAs) via high-throughput analytics provided by Thermo Scientific BioPharma Finder software. Connected labs can access data from development through QC using MAM 2.0 powered by Thermo Scientific Ardia Platform. And, PQAs can be monitored throughout the entire drug development process using the MAM target peptide workbook built from the list of identified peptides upon incorporation into a Chromeleon processing method. The processing method along with the instrument method, injection sequence, view setting and report template can be stored into a MAM eWorkflow for easy and direct transfer to QC via Chromeleon CDS Enterprise software.</p><div><h5><strong>MAM 2.0 offers:</strong></h5><ul><li>Fast decision making based on high-confidence PQAs obtained using industry-leading HRAM Orbitrap technology</li><li>Seamless knowledge and method transfer across instruments, departments, and sites using compliance-ready, Chromeleon CDS enterprise software, and Ardia Platform</li></ul><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Thermo_Scientific_Orbitrap_Exploris_MX_Mass_Detector_cd7474e7ed.jpg" alt="Thermo Scientific Orbitrap Exploris MX Mass Detector" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Thermo_Scientific_Orbitrap_Exploris_MX_Mass_Detector_cd7474e7ed.jpg 245w," sizes="100vw" width="1170" height="504"></p><blockquote><p><strong>Thermo Scientific MAM 2.0</strong></p><p>The end-to-end MAM solution for a fully connected, compliance-ready platform. MAM 2.0 includes everything you need to bring your next biotherapeutic candidate straight through to breakthrough.</p><ul><li><a target="_blank" rel="noopener noreferrer" href="https://www.thermofisher.com/cz/en/home/industrial/pharma-biopharma/biopharmaceutical-analytical-testing/multi-attribute-method.html"><strong>Learn more</strong></a></li></ul></blockquote></div></div></div></div></div>

Thermo Scientific Orbitrap Exploris Mass Spectrometers

Discover which Orbitrap Exploris mass spectrometry system will meet your business needs for more efficient screening, identification, confirmation and quantitation.

<p><strong>Benefit from record-setting performance. With built-in intelligence, sensitivity and selectivity; the Thermo Scientific Orbitrap Exploris mass spectrometry is quantitative high-resolution, accurate-mass (HRAM) liquid chromatography mass spectrometry (LC-MS) for analysis depth to the lowest levels with high quantitative accuracy and precision to meet your exact analytical requirements. Scale up your research and translate your results more rapidly and simply than ever before.</strong></p><h3><strong>Why choose an Orbitrap Exploris mass spectrometer for your laboratory?</strong></h3><p>Discover which Orbitrap Exploris mass spectrometry system will meet your business needs for more efficient screening (including targeted, suspected and nontargeted), identification, confirmation and quantitation.</p><h4><a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/products/1490"><strong>Orbitrap Exploris MX mass detector</strong></a></h4><p>Accelerate biopharmaceutical commercialization, manufacturing and quality control by easily and reliably obtaining compliance-ready, high-quality mass information with the Thermo Scientific Orbitrap Exploris MX Mass Detector.</p><p><strong>Typical applications: </strong>Biopharmaceutical characterization and QC, intact protein analysis, Multi-Attribute Method (MAM), oligonucleotides, pharmaceuticals</p><p><strong>Analyses: </strong>Targeted / non-targeted screening (MS), Unknown Identification (MS), Absolute and Relative Quantitation (MS)</p><p><strong>Resolution powers: </strong>180,000</p><p><strong>Mass range: </strong>m/z 40–3,000 up to m/z 8,000 with BioPharma option</p><p><strong>Scan speed: </strong>22Hz</p><p><strong>Sources &amp; Flexibility: </strong><a target="_blank" rel="noopener noreferrer" href="https://www.thermofisher.com/cz/en/home/industrial/mass-spectrometry/liquid-chromatography-mass-spectrometry-lc-ms/lc-ms-systems/orbitrap-lc-ms/orbitrap-exploris-mass-spectrometers.html#small">AP-SMALDI<sup>5</sup>&nbsp;AF source for MS Imaging</a></p><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Thermo_Scientific_Orbitrap_Exploris_MX_Mass_Detector_2_a84ed39672.jpg" alt="Thermo Scientific Orbitrap Exploris MX Mass Detector" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Thermo_Scientific_Orbitrap_Exploris_MX_Mass_Detector_2_a84ed39672.jpg 169w," sizes="100vw" width="1696" height="1575"></p><h4><a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/products/403"><strong>Orbitrap Exploris 120 Mass Spectrometer&nbsp;</strong></a></h4><p>Benefit from best in class high-resolution accurate mass for confident testing, screening and quantification of small molecules with the Thermo Scientific Orbitrap Exploris 120 Mass Spectrometer.&nbsp;&nbsp;</p><p><strong>Typical applications: </strong>Food and environmental testing, metabolomics, forensic toxicology, clinical research, pharmaceuticals</p><p><strong>Analyses: </strong>Targeted / non-targeted screening (MS), Unknown Identification (MS), Confirmation (MS/MS), Absolute and Relative Quantitation (MS and/or MS<sup>2</sup>)</p><p><strong>Resolution powers: </strong>120,000</p><p><strong>Mass range: </strong><i>m/z</i>&nbsp;40 – 3,000</p><p><strong>Scan speed: </strong>22Hz</p><p><strong>Sources &amp; Flexibility: </strong><a target="_blank" rel="noopener noreferrer" href="https://www.thermofisher.com/order/catalog/product/IQLAAEGABBFAGOMZZZ">APCI/APPI Combination</a>, <a target="_blank" rel="noopener noreferrer" href="https://www.thermofisher.com/cz/en/home/industrial/mass-spectrometry/liquid-chromatography-mass-spectrometry-lc-ms/lc-ms-systems/orbitrap-lc-ms/orbitrap-exploris-mass-spectrometers.html#small">AP-SMALDI<sup>5</sup>&nbsp;AF source for MS Imaging</a></p><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Thermo_Orbitrap_Exploris_120_Mass_Spectrometer_3_77e4e07555/Thermo-Orbitrap-Exploris-120-Mass-Spectrometer-3.jpg" alt="Thermo Orbitrap Exploris 120 Mass Spectrometer " srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Thermo_Orbitrap_Exploris_120_Mass_Spectrometer_3_77e4e07555/thumbnail_Thermo-Orbitrap-Exploris-120-Mass-Spectrometer-3.jpg 171w," sizes="100vw" width="938" height="856"></p><h4><a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/products/402"><strong>Orbitrap Exploris 240 Mass Spectrometer&nbsp;</strong></a></h4><p>Get unmatched confidence for discovery and identification, with application versatility and operational simplicity with the Thermo Scientific Orbitrap Exploris 240 Mass Spectrometer.&nbsp;</p><p><strong>Typical applications: </strong>Biopharmaceutical characterization, proteomics, metabolomics, lipidomics, food and environmental research, clinical research</p><p><strong>Analyses: </strong>Targeted / non-targeted screening (MS), Unknown Identification (MS), Confirmation (MS/MS), Absolute and Relative Quantitation (MS and/or MS<sup>2</sup>)</p><p><strong>Resolution powers: </strong>240,000</p><p><strong>Mass range: </strong><i>m/z</i>&nbsp;40 - 6000, (up to 8000 with BioPharma option)</p><p><strong>Scan speed: </strong>30Hz</p><p><strong>Sources &amp; Flexibility: </strong><a target="_blank" rel="noopener noreferrer" href="https://www.thermofisher.com/us/en/home/industrial/mass-spectrometry/liquid-chromatography-mass-spectrometry-lc-ms/lc-ms-accessories/field-asymmetric-ion-mobility-spectrometry-faims.html">FAIMS Pro Duo interface</a>, <a target="_blank" rel="noopener noreferrer" href="https://www.thermofisher.com/order/catalog/product/IQLAAEGABBFAGOMZZZ">APCI/APPI Combination</a>, <a target="_blank" rel="noopener noreferrer" href="https://www.thermofisher.com/order/catalog/product/ES081">EASY-Spray Source</a>, <a target="_blank" rel="noopener noreferrer" href="https://www.thermofisher.com/order/catalog/product/ES071">Nanospray Flex Ion Sources</a>&nbsp;, <a target="_blank" rel="noopener noreferrer" href="https://www.thermofisher.com/cz/en/home/industrial/mass-spectrometry/liquid-chromatography-mass-spectrometry-lc-ms/lc-ms-systems/orbitrap-lc-ms/orbitrap-exploris-mass-spectrometers.html#small">AP-SMALDI<sup>5</sup>&nbsp;AF source for MS Imaging</a></p><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Thermo_Orbitrap_Exploris_240_Mass_Spectrometer_2_a5085c34b0/Thermo-Orbitrap-Exploris-240-Mass-Spectrometer-2.jpg" alt="Thermo Orbitrap Exploris 240 Mass Spectrometer " srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Thermo_Orbitrap_Exploris_240_Mass_Spectrometer_2_a5085c34b0/thumbnail_Thermo-Orbitrap-Exploris-240-Mass-Spectrometer-2.jpg 175w," sizes="100vw" width="945" height="841"></p><h4><a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/products/401"><strong>Orbitrap Exploris 480 Mass Spectrometer&nbsp;</strong></a></h4><p>Achieve next-level insights, exploratory and targeted quantitation, next-gen power, performance, and ease of use with the Thermo Scientific Orbitrap Exploris 480 Mass Spectrometer.</p><p><strong>Typical applications: </strong>Shotgun proteomics (LFQ DIA, DDA), single cell proteomics, TMT multiplexing, targeted peptide quantification, metabolomics, biopharmaceutical applications (including HCP and protein characterization).</p><p><strong>Analyses: </strong>Targeted / non-targeted screening (MS), Unknown Identification (MS), Confirmation (MS/MS), Absolute and Relative Quantitation (MS and/or MS<sup>2</sup>)</p><p><strong>Resolution powers: </strong>480,000</p><p><strong>Mass range: </strong><i>m/z</i>&nbsp;40 - 6000, (up to 8000 with BioPharma option)</p><p><strong>Scan speed: </strong>40Hz</p><p><strong>Sources &amp; Flexibility: </strong><a target="_blank" rel="noopener noreferrer" href="https://www.thermofisher.com/us/en/home/industrial/mass-spectrometry/liquid-chromatography-mass-spectrometry-lc-ms/lc-ms-accessories/field-asymmetric-ion-mobility-spectrometry-faims.html">FAIMS Pro Duo interface</a>, <a target="_blank" rel="noopener noreferrer" href="https://www.thermofisher.com/order/catalog/product/IQLAAEGABBFAGOMZZZ">APCI/APPI Combination</a>, <a target="_blank" rel="noopener noreferrer" href="https://www.thermofisher.com/order/catalog/product/ES081">EASY-Spray Source</a>, <a target="_blank" rel="noopener noreferrer" href="https://www.thermofisher.com/order/catalog/product/ES071">Nanospray Flex Ion Sources</a>&nbsp;, <a target="_blank" rel="noopener noreferrer" href="https://www.thermofisher.com/cz/en/home/industrial/mass-spectrometry/liquid-chromatography-mass-spectrometry-lc-ms/lc-ms-systems/orbitrap-lc-ms/orbitrap-exploris-mass-spectrometers.html#small">AP-SMALDI<sup>5</sup>&nbsp;AF source for MS Imaging</a></p><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Thermo_Orbitrap_Exploris_480_Mass_Spectrometer_2_3d91c9b5b1/Thermo-Orbitrap-Exploris-480-Mass-Spectrometer-2.jpg" alt="Thermo Orbitrap Exploris 480 Mass Spectrometer " srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Thermo_Orbitrap_Exploris_480_Mass_Spectrometer_2_3d91c9b5b1/thumbnail_Thermo-Orbitrap-Exploris-480-Mass-Spectrometer-2.jpg 235w," sizes="100vw" width="880" height="584"></p>

Thermo Scientific Orbitrap Excedion Pro Mass Spectrometers

Welcome to the future of mass spectrometry. Enhance your research and achieve groundbreaking discoveries with Thermo Scientific Orbitrap Excedion Pro Hybrid Mass Spectrometers, the next generation of mass spectrometry.

Thermo Scientific Orbitrap Astral Zoom Mass Spectrometer

Orbitrap Astral Zoom boosts flexibility, speed, sensitivity, and quantitation for Omics and Biopharma with hybrid DIA, TMT HR modes, and next-gen acquisition.

Thermo Scientific Orbitrap Exploris EFOX Mass Detector

Orbitrap Exploris EFOX is an HRMS detector for screening, quantification, and profiling of trace contaminants in food and environmental analysis using full-scan acquisition and in-source CID.

<p><strong>The Thermo Scientific™ Orbitrap Exploris™ EFOX Mass Detector is an Orbitrap-based mass detector designed for accurate and reliable trace contaminant analysis in environmental and food safety applications. This high-resolution mass spectrometry system enables comprehensive screening, quantification, and sample profiling within a single analysis while supporting efficient laboratory workflows and regulatory compliance.</strong></p><p>The instrument combines high sensitivity, selectivity, and robust design with simplified workflows for both routine and advanced applications. Full-scan acquisition together with in-source fragmentation eliminates the need for time-consuming MS/MS method optimization. Integration with <a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/products/76"><strong>Thermo Scientific™ Chromeleon™ software</strong></a> ensures efficient data processing and management.</p><h4><strong>Key features</strong></h4><ul><li>High-resolution mass spectrometry for precise detection and quantification of trace contaminants</li><li>Supports targeted analysis, suspect screening, and comprehensive sample profiling</li><li>Streamlined workflows reducing operational and maintenance costs</li><li>Enables retrospective data analysis without sample reinjection</li><li>Integrated with <strong>Chromeleon™ CDS</strong> for efficient data processing</li></ul><h4><strong>Technical specifications</strong></h4><ul><li><strong>Dimensions (D × W × H):</strong> 763 × 534 × 703 mm (30 × 21 × 27.6 in), without data system, vacuum rough pumps, and optional items</li><li><strong>Mass accuracy:</strong><ul><li><strong>External calibration:</strong> &lt;3 ppm RMS drift over 24 h</li><li><strong>1-point calibration: </strong>&lt;3 ppm RMS drift over at least 4 weeks</li><li><strong>Internal lock mass:</strong> &lt;1 ppm RMS drift over 24 h</li><li><strong>EASY-IC: </strong>&lt;1 ppm RMS drift for at least 5 days</li></ul></li><li><strong>Mass resolution:</strong> Up to 90,000 at 200 m/z</li><li><strong>Scan rates:</strong> Up to 22 Hz at resolution setting 15,000</li><li><strong>Software compatibility:</strong> Chromeleon software</li><li><strong>Weight:</strong> 120 kg (265 lbs)</li><li><strong>Mass range:</strong> 50–1500 m/z</li><li><strong>Technique:</strong> MS</li><li><strong>Type:</strong> Orbitrap LC-MS</li><li><strong>Unit size:</strong> Each</li></ul>

Thermo Scientific Nanospray Flex Ion Source 

Thermo Scientific™ Nanospray Flex™ Ion Source is a flexible nano-electrospray ion source for LC-MS, delivering stable ionization, high sensitivity, and seamless integration with online and offline nano-ES workflows.

<p><strong>Thermo Scientific™ Nanospray Flex™ Ion Source is a nano-electrospray ion source designed to maximize LC-MS sensitivity while maintaining simple operation and exceptional flexibility. Developed for nanoflow LC-MS applications, it provides stable nano-electrospray ionization, low-flow operation, and flexible integration with a wide range of columns and emitters. The complete system is supplied with all required accessories for immediate installation.</strong></p><h3><strong>Key Features</strong></h3><h4><strong>High-Sensitivity Nano-ESI</strong></h4><ul><li>Maximizes LC-MS sensitivity using stable nanoflow electrospray ionization</li><li>Supports nanoscale flow rates for efficient ionization and reduced sample consumption</li><li>Delivers highly stable spray performance for long analytical runs and stable spray performance during extended runs</li></ul><h4><strong>Flexible Workflow</strong></h4><ul><li>Single configuration for all online nanoflow LC-MS applications</li><li>Quickly switches between online LC-MS and offline nano-ES workflows</li><li>DirectJunction™ adaptor supports a wide range of column and emitter connections</li><li>Compatible with both stainless steel and glass emitters</li></ul><h4><strong>Easy Operation</strong></h4><ul><li>Precision X-Y-Z manipulator for accurate emitter positioning</li><li>Sliding rail design allows fast emitter access and replacement</li><li>Integrated camera, monitor, and illumination simplify emitter alignment</li><li>Supplied as a complete kit with accessories for immediate installation</li></ul><h3><strong>Instrument Compatibility</strong></h3><h4><strong>Original Nanospray Flex Ion Source</strong></h4><ul><li>Thermo Scientific™ LCQ Deca XP MAX™, LTQ™-series, and Velos™-series ion trap mass spectrometers</li><li>Thermo Scientific™ LTQ Orbitrap™-series and Orbitrap Velos™-series hybrid ion trap-Orbitrap mass spectrometers</li><li>Thermo Scientific™ Exactive™-series Orbitrap mass spectrometers</li><li>Thermo Scientific™ Q Exactive™-series hybrid quadrupole-Orbitrap mass spectrometers</li><li>Thermo Scientific™ TSQ Quantum™-series and TSQ Vantage™ triple-stage quadrupole mass spectrometers</li></ul><h4><strong>Nanospray Flex NG</strong></h4><ul><li><a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/products/562"><strong>Thermo Scientific™ Orbitrap Fusion™ Tribrid™ mass spectrometer</strong></a></li><li>Thermo Scientific™ TSQ Endura™ triple-stage quadrupole mass spectrometer</li></ul>

Thermo Scientific EASY-Spray Sources

Thermo Scientific™ EASY-Spray™ Sources are integrated nano-electrospray ion sources for nano-LC-MS featuring a temperature-controlled column-emitter assembly for stable ionization, excellent reproducibility, and simplified installation.

<p><strong>Thermo Scientific™ EASY-Spray™ Sources are integrated nano-electrospray ion sources designed for nanoflow LC-MS. Their temperature-controlled column-emitter assembly and single nanoViper™ connection simplify nanoLC-MS operation while delivering highly reproducible separations, stable spray performance, and high LC-MS sensitivity</strong></p><h4><strong>Integrated Design</strong></h4><ul><li>Integrated temperature-controlled column and emitter assembly</li><li>Single nanoViper™ connection between the LC system and ion source</li><li>Integrated zero-dead-volume (ZDV) connections minimize dead volume</li><li>Simple plug-and-spray installation with quick column replacement</li></ul><h4><strong>Chromatographic Performance</strong></h4><ul><li>Stable nano-electrospray ionization for reliable LC-MS analysis</li><li>Supports long columns and small particle sizes for high peak-capacity separations</li><li>Column heating (30–60 °C) reduces solvent viscosity and backpressure</li><li>Ultra-sharp chromatographic peaks improve separation efficiency</li></ul><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Thermo_Scientific_EASY_Spray_Sources_4_361e3bad42.jpg" alt="Thermo Scientific EASY-Spray Sources: The entire assembly is designed for heavy use so you can utilize the full pressure range of your UHPLC front-end (up to 1000 bar) and benefit from small particles and long columns. Longer columns and longer gradients both contribute to more identifications." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Thermo_Scientific_EASY_Spray_Sources_4_361e3bad42.jpg 229w," sizes="100vw" width="921" height="628"></p><h4><strong>Reproducibility</strong></h4><ul><li>Stable performance during extended analytical operation</li><li>Excellent run-to-run reproducibility</li><li>Outstanding column-to-column reproducibility for large studies and multi-site laboratories</li></ul><h4><strong>Ease of Use</strong></h4><ul><li>Plug-and-spray operation requiring minimal setup</li><li>Integrated camera provides an illuminated, magnified view of the emitter tip</li><li>Eliminates the most error-prone nanoLC connections</li></ul><h4><strong>Compatibility</strong></h4><p>Compatible with Thermo Scientific EASY-Spray columns and selected Thermo Scientific nanoLC systems and mass spectrometers, including:</p><ul><li>Thermo Scientific™ LCQ Deca XP MAX™, LTQ™-series, and Velos™ series ion trap mass spectrometers</li><li>Thermo Scientific™ LTQ Orbitrap™-series and Orbitrap Velos™ series hybrid ion trap-Orbitrap mass spectrometers</li><li>Thermo Scientific™ Exactive™ series Orbitrap mass spectrometers</li><li>Thermo Scientific™ Q Exactive™ series hybrid quadrupole-Orbitrap mass spectrometers</li><li>Thermo Scientific™ TSQ Quantum™ series and TSQ Vantage™ triple-stage quadrupole mass spectrometers</li></ul><p>The EASY-Spray NG ion source works in conjunction with the latest next-generation Thermo Scientific mass spectrometers:</p><ul><li><a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/products/562"><strong>Thermo Scientific™ Orbitrap™ Fusion™ Tribrid™ Mass Spectrometer</strong></a></li><li>Thermo Scientific™ TSQ Endura™ Triple-stage Quadrupole Mass Spectrometer</li><li>Thermo Scientific™ TSQ Quantiva™ Triple-stage Quadrupole Mass Spectrometer</li></ul>

Thermo Scientific OptiSpray Ion Source

The Thermo Scientific™ OptiSpray™ Ion Source is an intelligent ESI interface for nano- and capillary LC-MS, combining automated spray optimization, high sensitivity, reproducibility, and easy operation with the OptiSpray™ Cartridge system.

<p><strong>The Thermo Scientific™ OptiSpray™ Ion Source is an intelligent electrospray ionization interface designed to simplify the acquisition of high-quality, reproducible nano- and capillary LC-MS data. Compatible with the </strong><a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/products/398"><strong>Thermo Scientific™ Vanquish™ Neo UHPLC system </strong></a><strong>and the </strong><a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/products?category=5&amp;manufacturerItems=3"><strong>Thermo Scientific mass spectrometers </strong></a><strong>portfolio, it combines automated spray optimization with cartridge-based nano and capillary columns to deliver high sensitivity, quantitative accuracy, precision, and robust performance for discovery and targeted assays.</strong></p><h4><strong>High Performance</strong></h4><p>The OptiSpray™ ion source combines optimized fluidics with integrated temperature and sheath gas control to ensure stable, reproducible electrospray ionization. Automated spray positioning helps deliver reproducible, high-quality analytical results with or without the FAIMS interface.</p><h4><strong>Simple and Automated Operation</strong></h4><p>Designed for ease of use, the OptiSpray™ system features plug-and-play cartridge installation that virtually eliminates manual setup of nano and capillary columns. Automated spray optimization further simplifies operation, while onboard cartridge memory stores cartridge properties, usage data, and previous source settings, enabling rapid setup and consistent ESI performance over time.</p><h4><strong>Reliable and Reproducible Results</strong></h4><p>The source enhances robustness by automatically moving the spray to an inactive position between injections, helping maintain a cleaner MS system. Exchangeable emitters extend column lifetime, while automated spray positioning, cartridge memory, and integrated nebulization gas boost contribute to run-to-run and intercolumn reproducibility.</p><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Thermo_Scientific_Opti_Spray_Ion_Source_2_fd937ef73f.jpg" alt="Thermo Scientific OptiSpray Ion Source" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Thermo_Scientific_Opti_Spray_Ion_Source_2_fd937ef73f.jpg 156w," sizes="100vw" width="650" height="650"></p><h3><strong>Key Features</strong></h3><ul><li>Intelligent electrospray ionization interface for nano- and capillary LC-MS</li><li>Automated spray optimization for consistent ESI performance</li><li>Plug-and-play cartridge installation with minimal manual setup</li><li>Optimized fluidics with integrated temperature and sheath gas control</li><li>Automated spray positioning</li><li>Compatible with or without the FAIMS interface</li><li>Onboard cartridge memory storing cartridge properties, usage data, and previous source settings</li><li>Automatic inactive spray position between injections for improved system robustness</li><li>Exchangeable emitters to extend column lifetime</li><li>Integrated nebulization gas boost for improved reproducibility</li><li>Designed for discovery and targeted assays</li></ul>

Thermo Scientific Orbitrap Astral Mass Spectrometer

A new horizon for single-cell, quantitative, and translational proteomics.

<div><div container-id="cq-colctrl-lt1" comp-id="A1"><div><p><strong>Looking to unlock additional insights into biology and disease mechanisms? With faster throughput, deeper coverage, higher sensitivity, and accurate and precise quantitation; the Thermo Scientific Orbitrap Astral Mass Spectrometer enables you to dramatically expand the scale and scope of your experiments.</strong></p><p>See how it sets a new standard for mass spectrometry performance and redefines what is possible for discovery and translational research to help you rapidly and comprehensively capture the dynamic, temporal, and spatial complexity of biology.</p><h3><strong>Key benefits</strong></h3><h4><strong>Faster throughput</strong></h4><p>Achieve faster throughput with a high throughput injection-to-injection cycle of just 8 minutes.</p><ul><li>Analyze one sample in only 8 minutes</li><li>In one day, analyze over 1.4 million protein groups from 180 samples</li></ul><h4><strong>Deeper coverage</strong></h4><p>The near whole-proteome coverage of these methods reduces the chances of missing out on important proteins of interest.</p><ul><li>See more in less time or spend more time and go even deeper</li><li>Double the depth and quadruple the throughput for phosphoproteomics</li></ul><h4><strong>Higher sensitivity</strong></h4><p>The high dynamic range of the proteome necessitates the measurement of not only high abundance proteins, but also proteins with just a few copies.</p><ul><li>Deeper coverage from single cells at faster throughput</li><li>1 ng HeLa DIA/18 min (80 SPD)</li></ul><h4><strong>Accurate and precise quantitation</strong></h4><p>Biological changes are quantitative in nature, requiring accurate and precise measurements to determine statistically significant biomarkers.</p><ul><li>Accurate and precise quantitation of three-proteome mixtures</li><li>The Orbitrap Astral mass spectrometer enables accurate and precise quantitation with 88% of identified proteins having 20% CV or less and 75% having 10% CV or less</li></ul><p><img style="aspect-ratio:1224/513;" src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Pragolab_Konstrukce_hmotnostniho_spektrometru_Thermo_Orbitrap_Astral_e38e10580c/Pragolab-Konstrukce-hmotnostniho-spektrometru-Thermo-Orbitrap-Astral.jpg" alt="Thermo Fisher Scientific: Thermo Scientific Orbitrap Astral Mass Spectrometer" width="1224" height="513"></p><h3><a target="_blank" rel="noopener noreferrer" href="https://www.thermofisher.com/cz/en/home/industrial/mass-spectrometry/liquid-chromatography-mass-spectrometry-lc-ms/lc-ms-systems/orbitrap-lc-ms/orbitrap-astral-mass-spectrometer.html"><strong>An overview</strong></a><strong> of the Orbitrap Astral MS from Alexander Makarov, Ph.D.</strong></h3><p>The new Astral analyzer builds on Thermo Fisher Scientific’s proven Orbitrap mass spectrometry platform with novel technology to deliver up to two times deeper proteome coverage and up to four times more throughput compared to current mass spectrometers.</p><p>Alexander Makarov, Ph.D., Director Global Research LSMS, Thermo Fisher Scientific, claims the Astral analyzer represents a new class of high-resolution accurate-mass analyzers with a unique performance envelope.</p><p>"The introduction of the Astral analyzer represents a starting point in the future evolution of its technology to new frontiers of performance," said Makarov.</p><h3><strong>What customers are saying</strong></h3><h4><strong>A New Standard for Data-Independent Acquisition</strong></h4><p>Learn how the Orbitrap Astral mass spectrometer will enable precision medicine at scale with revolutionary new DIA methods for plasma proteomics.</p><p>The future of proteomics lies in the accurate and precise measurement of thousands of proteins across thousands of samples. Data-independent Acquisition (DIA) facilitates deep proteome coverage with high quantitative accuracy and precision using label-free quantitation (LFQ) due to the unbiased and reproducible nature of mass spectrometry measurements.</p><div><div container-id="cq-colctrl-lt0" comp-id="A1"><div><ul><li><a target="_blank" rel="noopener noreferrer" href="https://www.thermofisher.com/cz/en/home/industrial/mass-spectrometry/liquid-chromatography-mass-spectrometry-lc-ms/lc-ms-systems/orbitrap-lc-ms/orbitrap-astral-mass-spectrometer/why-orbitrap-astral.html?videoId=6331001711112">Mike MacCoss, Ph.D.</a>, Professor, Department of Genome Sciences, University of Washington</li><li><a target="_blank" rel="noopener noreferrer" href="https://www.thermofisher.com/cz/en/home/industrial/mass-spectrometry/liquid-chromatography-mass-spectrometry-lc-ms/lc-ms-systems/orbitrap-lc-ms/orbitrap-astral-mass-spectrometer/why-orbitrap-astral.html?videoId=6331014977112">Jennifer Van Eyk, Ph.D.</a>, Director, Basic Science Research, Barbra Streisand Women’s Heart Center Director, Advanced Clinical Biosystem Institute Center, Cedars-Sinai</li><li><a target="_blank" rel="noopener noreferrer" href="https://www.thermofisher.com/cz/en/home/industrial/mass-spectrometry/liquid-chromatography-mass-spectrometry-lc-ms/lc-ms-systems/orbitrap-lc-ms/orbitrap-astral-mass-spectrometer/why-orbitrap-astral.html?videoId=6331000425112">Jesper Olsen, Ph.D.</a>, Deputy Center Director, Professor, Novo Nordisk Foundation Center for Protein Research, University of Copenhagen</li><li><a target="_blank" rel="noopener noreferrer" href="https://www.thermofisher.com/cz/en/home/industrial/mass-spectrometry/liquid-chromatography-mass-spectrometry-lc-ms/lc-ms-systems/orbitrap-lc-ms/orbitrap-astral-mass-spectrometer/why-orbitrap-astral.html?videoId=6331001022112">Qing Yu</a>, Research Associate at Harvard Medical School</li></ul></div></div></div><h4><strong>The Era of Single-Cell Proteomics Has Arrived</strong></h4><p>Single cell proteomics can determine the dynamic nature of the proteome across cell types in both time and space. The increased resolution that is gained by measuring single cells instead of bulk samples provides a deeper insight into disease mechanisms and fundamental biological processes to unravel the complexity of biology. Learn how the Orbitrap Astral mass spectrometer opens a new horizon for single-cell proteomics by empowering higher throughput and deeper coverage without sacrificing quantitative accuracy and precision.</p><div><div container-id="cq-colctrl-lt0" comp-id="B1"><div><ul><li><a target="_blank" rel="noopener noreferrer" href="https://www.thermofisher.com/cz/en/home/industrial/mass-spectrometry/liquid-chromatography-mass-spectrometry-lc-ms/lc-ms-systems/orbitrap-lc-ms/orbitrap-astral-mass-spectrometer/why-orbitrap-astral.html?videoId=6331010858112">Erwin Schoof, Ph.D.</a>, Associate Professor, Department of Biotechnology and Biomedicine, Section for Protein Science and Biotherapeutics, Cell Diversity Lab, Technical University of Denmark</li><li><a target="_blank" rel="noopener noreferrer" href="https://www.thermofisher.com/cz/en/home/industrial/mass-spectrometry/liquid-chromatography-mass-spectrometry-lc-ms/lc-ms-systems/orbitrap-lc-ms/orbitrap-astral-mass-spectrometer/why-orbitrap-astral.html?videoId=6331001916112">Joshua Coon, Ph.D.</a>, Professor of Biomolecular Chemistry at the University of Wisconsin-Madison</li><li><a target="_blank" rel="noopener noreferrer" href="https://www.thermofisher.com/cz/en/home/industrial/mass-spectrometry/liquid-chromatography-mass-spectrometry-lc-ms/lc-ms-systems/orbitrap-lc-ms/orbitrap-astral-mass-spectrometer/why-orbitrap-astral.html?videoId=6331012816112">Nikolai Slavov, Ph.D.</a>, Founding Director of Parallel Squared Technology Institute and Professor at Northeastern University</li><li><a target="_blank" rel="noopener noreferrer" href="https://www.thermofisher.com/cz/en/home/industrial/mass-spectrometry/liquid-chromatography-mass-spectrometry-lc-ms/lc-ms-systems/orbitrap-lc-ms/orbitrap-astral-mass-spectrometer/why-orbitrap-astral.html?videoId=6331016836112">Sophia Steigerwald, Ph.D.</a>, Student at the Max Planck Institute of Biochemistry</li></ul></div></div></div><h3><strong>How it works</strong></h3><p>The Orbitrap Astral Mass Spectrometer combines three mass analyzers: a quadrupole mass analyzer for high selectivity and high ion transmission; an Orbitrap mass analyzer for high dynamic range and high-resolution measurements; and a novel Astral analyzer for fast and sensitive measurements. The front end, from the ion source up to the segmented quadrupole mass filter, is designed to maximize instrument sensitivity and robustness. Ions are trapped and concentrated in the ion routing multipole, which facilitates the transfer of ions to either of the high-resolution accurate-mass (HRAM) analyzers. Synchronization of ion transfer and processing throughout the instrument enables the parallel handling of five separate ion packets simultaneously. This synchronization enables each mass analyzer to be used in combination to optimize performance and experimental flexibility.</p><p>The Orbitrap analyzer is ideally suited for the acquisition of high dynamic range HRAM MS and MS<sup>2</sup> spectra, including an optional Biopharma mode to extend the mass range up to <i>m/z</i> 8,000. The Astral Mass Analyzer delivers high acquisition rates up to 200 Hz, single ion detection sensitivity, and high dynamic range HRAM SIM and MS<sup>2</sup> measurements that are fully synchronized with parallel Orbitrap analyzer operation. Due to this synchronization the Orbitrap Astral Mass Spectrometer excels at many data acquisition strategies including High Resolution Data-Independent Acquisition (HR-DIA) and data-dependent acquisition (DDA). These acquisition strategies combined with the high resolution and dynamic range of the Orbitrap and Astral analyzers enable accurate and precise quantitation using either Label-Free Quantitation (LFQ) or multiplexing with Tandem Mass Tag (TMT)-labeled peptides.</p></div></div></div><p class="cke-user_style-important"><strong>A 3D tour of the instrument can be found </strong><a target="_blank" rel="noopener noreferrer" href="https://www.thermofisher.com/cz/en/home/virtual/orbitrap-astral-ms-3d-tour.html"><strong>here</strong></a><strong>.</strong></p>

Products focused on LC/Orbitrap - page 1

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