<p><strong>Quantifying steroids in biological matrices has always presented analytical challenges. Complex structures, isobaric interferences, and matrix effects often limit accuracy and throughput — demanding a new approach beyond incremental improvements.</strong></p><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Thermo_Fisher_Scientific_Advancing_Steroid_Quantification_Unlocking_Sensitivity_and_Selectivity_with_the_Thermo_Scientific_Stellar_Mass_Spectrometer_6e03ce7f59.jpg" alt="Thermo Fisher Scientific: Advancing Steroid Quantification: Unlocking Sensitivity and Selectivity with the Thermo Scientific Stellar Mass Spectrometer." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Thermo_Fisher_Scientific_Advancing_Steroid_Quantification_Unlocking_Sensitivity_and_Selectivity_with_the_Thermo_Scientific_Stellar_Mass_Spectrometer_6e03ce7f59.jpg 245w," sizes="100vw" width="1024" height="512"></p><p>The<a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/products/1149"> <strong>Thermo Scientific Stellar MS</strong></a> redefines how analysts approach <strong>steroid quantification</strong>, enabling rapid method optimization through advanced scanning flexibility, complementary fragmentation mechanisms, and enhanced selectivity.</p><p>Rather than focusing on a single metric, this application note demonstrates how researchers can <strong>quickly develop more effective methods</strong> by leveraging the full capability of the Stellar MS to characterize compounds comprehensively in a single acquisition.</p><h3><strong>A new paradigm for analytical quantitation</strong></h3><p>Traditional LC-MS approaches often rely on incremental improvements in sensitivity or speed.<br>Stellar MS instead provides a new analytical framework, designed to optimize every parameter of quantitative method creation.</p><p><strong>Key attributes include:</strong></p><ol><li><strong>PRM vs. SRM/MRM — improving instrument efficiency</strong><br>Parallel Reaction Monitoring (PRM) on Stellar MS provides comprehensive ion information for each target steroid, improving quantitation accuracy and efficiency over traditional MRM methods.</li><li><strong>Complementary dissociation mechanisms — HCD and CID</strong><br>Stellar’s unique ability to combine <strong>beam-type (HCD)</strong> and <strong>resonant (CID)</strong> dissociation ensures full coverage of all 17 targeted steroids. This dual-mode approach effectively overcomes neutral water loss product ions — one of the key challenges in steroid analysis.</li><li><strong>Next-level selectivity — MS² to MS³ transition</strong><br>The platform’s ability to perform <strong>MS³ experiments</strong> enhances selectivity and confidence, particularly in complex biological matrices, where interferences are common.</li><li><strong>Product ion accumulation vs. dwell time — improved ion ratios and sensitivity</strong><br>By accumulating product ions rather than relying on fixed dwell times, Stellar MS achieves superior ion ratio precision and reproducibility — even at low spiking levels — without sacrificing throughput.</li></ol><p>&nbsp;<img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Thermo_Fisher_Scientific_Advancing_Steroid_Quantification_Unlocking_Sensitivity_and_Selectivity_with_the_Thermo_Scientific_Stellar_Mass_Spectrometer_2_21e509451a.jpg" alt="Thermo Fisher Scientific: Advancing Steroid Quantification: Unlocking Sensitivity and Selectivity with the Thermo Scientific Stellar Mass Spectrometer: Figure 1. Representative EIC of 17 steroids in human serum using the Thermo Scientific Stellar mass spectrometer." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Thermo_Fisher_Scientific_Advancing_Steroid_Quantification_Unlocking_Sensitivity_and_Selectivity_with_the_Thermo_Scientific_Stellar_Mass_Spectrometer_2_21e509451a.jpg 245w," sizes="100vw" width="800" height="412"></p><h3><strong>From characterization to confident method creation</strong></h3><p>The power of Stellar MS lies in its <strong>comprehensive acquisition strategy</strong>. In this application note, a single injection per spiking level generated <strong>125 distinct scan events</strong>, combining:</p><ul><li>HCD and CID MS² experiments</li><li>Targeted MS³ scans</li><li>Positive/negative <strong>polarity switching</strong></li></ul><p><strong>This workflow enables analysts to rapidly evaluate:</strong></p><ol><li>Which <strong>dissociation mechanism</strong> produces the most informative spectra</li><li>Whether <strong>MS² or MS³</strong> is best suited for each compound</li><li>The optimal <strong>product ions</strong> for confident post-acquisition quantitation</li></ol><p>By providing this complete dataset in a single run, Stellar MS dramatically accelerates <strong>method optimization and validation</strong>, supporting more efficient and reliable quantitation strategies.</p><h3><strong>Driving method development efficiency</strong></h3><p>The real strength of the Stellar MS isn’t just its speed or sensitivity — it’s the ability to <strong>experiment, compare, and decide</strong> in one workflow.<br><br>Through simultaneous HCD/CID fragmentation, MS² and MS³ switching, and polarity control, users can determine the optimal analytical conditions across complex steroid panels quickly and confidently.</p><p><strong>This approach helps labs:</strong></p><ul><li>Reduce development time</li><li>Maximize data quality</li><li>Improve long-term robustness and reproducibility</li></ul><h3><strong>Conclusion</strong></h3><p>The <strong>Stellar MS</strong> platform empowers analytical scientists to move beyond incremental performance metrics toward <strong>methodological innovation</strong>.</p><p>By integrating comprehensive characterization and advanced scanning control, it enables faster, more confident decisions in quantitative steroid analysis — setting a new benchmark for flexibility and precision in LC-MS.</p><p><strong>Ask the Experts: On-Demand Webinar</strong></p><p>Learn more about how Stellar MS enhances steroid profiling and quantitative workflows.<br><strong>Watch the on-demand webinar where Thermo Fisher scientists discuss:</strong></p><ul><li>Advanced method development strategies</li><li>Insights from the steroid panel dataset</li><li>Practical examples of leveraging Stellar’s flexibility for confident quantitation</li></ul><blockquote><p><strong>Watch now: </strong><a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/webinar/5117"><strong>Unlocking the Power of Quantitative Analysis with Stellar MS</strong></a></p><p><strong>Read the full application note:</strong><i><strong> </strong></i><a target="_blank" rel="noopener noreferrer" href="https://lcms.cz/paper/32415"><i><strong>Selective and sensitive measurement of 17 steroids in human serum using a Stellar mass spectrometer</strong></i></a></p></blockquote><ul><li>Learn more about<strong> </strong><a target="_blank" rel="noopener noreferrer" href="https://www.thermofisher.com/content/lifetech/global/en/home/industrial/mass-spectrometry/liquid-chromatography-mass-spectrometry-lc-ms/lc-ms-systems/orbitrap-lc-ms/stellar-mass-spectrometer/stellar-mass-spectrometer-applications.html"><strong>Stellar Mass Spectrometer applications</strong></a></li><li><strong>Visit us on&nbsp;</strong><a target="_blank" rel="noopener noreferrer" href="https://www.linkedin.com/showcase/72003132/admin/dashboard/"><strong><u>LinkedIn:</u></strong></a><strong>&nbsp;</strong>#MassSpectrometry #ClinicalResearch #LCMS #StellarMS</li></ul><blockquote><h5><strong>Dane Walker</strong></h5><p>Dane Walker is Regional Marketing Manager at Thermo Fisher Scientific, focused on LC-MS systems across EMEA. With a PhD in Surface Science and a background in scientific instrumentation, he blends technical expertise with strategic marketing to drive data-led campaigns. Passionate about communicating science and enabling innovation, Dane enjoys powerboating, cycling, stargazing, and talking endlessly about mass spectrometry.</p></blockquote>

Advancing Steroid Quantification: Unlocking Sensitivity and Selectivity with the Thermo Scientific Stellar Mass Spectrometer

<h4><strong>The problem&nbsp;</strong></h4><p>We live in a “global village” but we are all different and we use many different languages to communicate.&nbsp;</p><p>Even when people speak the same language, the same words may be used with different meanings. See, for example, some different uses of the word “standard” in English – a normative document, a solution of known concentration or even a type of flag.&nbsp;</p><p>Even in the same area of activity misunderstandings do occur which may result in a waste of time and money, or even worse consequences.&nbsp;</p><p>When talking about laboratory tests, lack of a full understanding of key terms and inappropriate translations leaves room for different interpretations of stated requirements. For example, there have historically been a number of different but related uses of the term ‘<strong>detection limit’</strong>. This may lead to inadequate fulfilment of those requirements, thus becoming an obstacle to the production of analytical data that is fit for its intended use. A shared interpretation of concepts related to quality in measurement between laboratories and accreditation bodies is also crucial for a fair and harmonised approach to laboratory assessment across the world.&nbsp;</p><h4><strong>A common language&nbsp;</strong></h4><p>We need a <strong>common language</strong>; clear and unambiguous. We need a common vocabulary to provide consistent definitions of concepts with their associated terms. To this end, several organisations participate in a joint effort to develop and maintain an <strong>international vocabulary of metrology</strong> (VIM) [1], which aims to achieve a unique understanding of concepts related to measurements across all sectors. The VIM is a normative reference in the standards <strong>ISO/IEC 17025, ISO 15189 and ISO/IEC 17043</strong>, and is therefore a key document for all organisations seeking accreditation.&nbsp;</p><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Eurachem_You_talk_we_understand_The_way_out_of_the_tower_of_Babel_Standards_8d947dbd5f.jpg" alt="Eurachem: You talk, we understand – The way out of the tower of Babel_A common language" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Eurachem_You_talk_we_understand_The_way_out_of_the_tower_of_Babel_Standards_8d947dbd5f.jpg 245w," sizes="100vw" width="1126" height="499"></p><h4><strong>Is this enough?&nbsp;</strong></h4><p>For laboratory staff some problems still stand and need to be addressed:&nbsp;</p><ul><li>Many people are confused about both concepts and terms.&nbsp;</li><li>It may be difficult to understand the “formal” VIM definitions – they are short statements intended to apply to many different measurement sectors.</li><li>Translations into local languages may add to the confusion, e.g. if different terms are used in different sectors for the same VIM concept and the translator overlooks this issue.</li><li>Some substantial changes to terminology have been made in an attempt to accommodate chemical and biological measurements. Some “old” concepts still stand but have new names, e.g. “within laboratory reproducibility” vs. “intermediate precision”.&nbsp;</li></ul><p>To address these issues Eurachem has produced a guide [2] which provides an accessible reference containing simple and in context explanations of terms most relevant to analysts, with additional examples relating those concepts to chemical and biological measurements.&nbsp;</p><h4><strong>Do you understand the meaning of these terms?&nbsp;</strong></h4><p>Are you interested in a <strong>quantity</strong> or a quality? Depending on the answer, will you use a <strong>measurement</strong> <strong>procedure</strong> or an examination? Is method <strong>verification</strong> the same or less demanding than method <strong>validation</strong>? Do you need a <strong>calibration</strong> or just performance verification? Do you have a <strong>measurement standard</strong> in your laboratory? Is <strong>metrological traceability</strong> something you worry about? These are examples of the terms supporting the reliability of any measurement result that need to be clearly understood.&nbsp;</p><h5><strong>Example – Accuracy, trueness, precision: are they synonyms?&nbsp;</strong></h5><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Eurachem_You_talk_we_understand_The_way_out_of_the_tower_of_Babel_accuracy_231e1e005e.jpg" alt="Eurachem: You talk, we understand – The way out of the tower of Babel_Example – Accuracy, trueness, precision" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Eurachem_You_talk_we_understand_The_way_out_of_the_tower_of_Babel_accuracy_231e1e005e.jpg 146w," sizes="100vw" width="754" height="807"></p><ul><li>Closeness between measurement results (relating to the concept of precision)</li><li>Closeness of the average of the measurement results to a reference value (relating to the concept of trueness)&nbsp;</li><li>Closeness of an individual measurement result to the true value (relating to the concept of accuracy)&nbsp;</li></ul><p>The different situations in the figure show the effect of:&nbsp;</p><ol style="list-style-type:lower-latin;"><li>poor precision and trueness (imprecise and biased)&nbsp;</li><li>good precision, poor trueness (precise but biased)&nbsp;</li><li>poor precision, good trueness (imprecise but unbiased)&nbsp;</li><li>good precision and trueness (precise and unbiased)&nbsp;</li></ol><p>Improving both precision and trueness is expected to improve accuracy.&nbsp;</p><h4><strong>Is all this relevant to you?&nbsp;</strong></h4><p>Do you work in a laboratory, either as an analyst, quality manager or director? Are you involved in other activities related to analytical work, such as providing proficiency tests, producing reference materials or accrediting any such activity? Do you teach or study subjects related to measurements? Do you use measurement results or prescribe how to use them? <i><strong>If the answer is yes to any of these questions, the following reference documents are also for you</strong></i>.</p>

You talk, we understand – The way out of the tower of Babel

<p><strong>Visit the LabRulez booth at </strong><a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/events/871"><strong>analytica 2026</strong></a><strong> in Munich, 24. - 27. March 2026! You can find us in Hall A1, Booth A1.506. At the booth, you will be able to explore live demonstrations of how the LabRulez portals work and see selected analytical instruments from our pre-owned marketplace section.</strong></p><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Lab_Rulez_at_Analytica_2026_Floor_plan_55e1057e03.jpg" alt="LabRulez at Analytica 2026: Hall A1 Floor Plan and Booth Location" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Lab_Rulez_at_Analytica_2026_Floor_plan_55e1057e03.jpg 245w," sizes="100vw" width="1516" height="881"></p><h3><strong>Would you like to learn how the LabRulez portals work?</strong></h3><p>➡︎ <a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/"><strong>LabRulezLCMS</strong></a><br>➡︎ <a target="_blank" rel="noopener noreferrer" href="https://gcms.labrulez.com/"><strong>LabRulezGCMS</strong></a><br>➡︎ <a target="_blank" rel="noopener noreferrer" href="https://icpms.labrulez.com/"><strong>LabRulezICPMS</strong></a></p><ul><li>A <strong>unique online solution</strong> for <strong>your marketing</strong></li><li>A <strong>targeted audience</strong> from the <strong>analytical chemistry community</strong></li><li><strong>Modern technologies</strong> and a unique concept</li><li><strong>Design optimized for most smart devices</strong> – PC, mobile, and tablet</li><li><strong>Advanced statistical tools</strong> fully available to users</li></ul><h3><strong>Services Offered</strong></h3><h4><strong>News + Company Updates</strong></h4><ul><li>Publish <strong>news related to your instrumentation, applications, services, events, job openings</strong>, and more</li><li><strong>News is shared</strong> both on our <strong>portals</strong> and across our <strong>social media channels</strong></li></ul><h4><strong>Library – All Documents in One Place</strong></h4><ul><li><strong>Applications, posters, presentations, technical articles, brochures, specifications</strong></li><li><strong>Fas</strong>t and <strong>advanced search</strong> capabilities</li><li>Over <strong>32,000 documents</strong> currently available in our database</li></ul><h4><strong>Webinars</strong></h4><ul><li><strong>Upcoming and on-demand webinars</strong> from the field of analytical chemistry</li><li>More than <strong>5,500 webinars</strong> covering chromatography, mass spectrometry, and spectroscopy</li></ul><h4><strong>Careers</strong></h4><ul><li><strong>Targeted job</strong> listings in <strong>analytical chemistry</strong></li><li>Clear filtering by specialization or location</li><li><strong>Increased visibility for job postings</strong></li></ul><h4><strong>Companies</strong></h4><ul><li><strong>Company profile</strong> with key information and contacts</li><li><strong>Integration of all your activities on our portal:</strong> News, Library documents, Webinars, Upcoming Events, Careers, and mentions by partners or customers</li><li><strong>Connection to your Products </strong>listed on LabRulez portals</li></ul><h4><strong>Products and Services</strong></h4><ul><li>Comprehensive <strong>overview of instrumental techniques and services</strong></li><li>Targeting the analytical chemistry community and customers in the growing online environment</li></ul><h4><strong>Marketplace</strong></h4><ul><li><strong>Buying and selling pre-owned analytical equipment</strong></li><li>We purchase analytical instrumentation of all brands and ages</li><li><strong>Sell your equipment through LabRulez</strong></li><li>Request and purchase <strong>complete systems, modules</strong>, or <strong>spare parts</strong> with warranty and service</li></ul><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Lab_Rulez_at_analytica_2026_bazar_7edf4a0872.jpg" alt="LabRulez at analytica 2026" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Lab_Rulez_at_analytica_2026_bazar_7edf4a0872.jpg 88w," sizes="100vw" width="1600" height="2844"></p><h3><strong>What Will Be on Display at Our Booth?</strong></h3><p>Come and explore selected spare parts and modules from our marketplace section. These include both new, tested, and pre-owned items.</p><h4><a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/bazaar"><strong>LabRulezLCMS Marketplace</strong></a></h4><p><strong>Spare parts for LC/MS:</strong></p><ul><li><a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/bazaar?category=378"><strong>Vacuum turbo pumps for LC/MS</strong></a></li></ul><p><strong>Spare parts for HPLC:</strong></p><ul><li><a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/bazaar?category=397"><strong>Degassers for HPLC/UHPLC</strong></a></li><li><a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/bazaar?category=368&amp;search=valve"><strong>Automatic valves for HPLC</strong></a></li><li>HPLC components for Agilent 1100, 1200, and 1260 Infinity II series</li></ul><p><strong>Consumables:</strong></p><ul><li><a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/bazaar?category=377"><strong>HPLC lamps</strong></a></li><li><a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/bazaar?category=48"><strong>HPLC columns</strong></a></li></ul><h4><a target="_blank" rel="noopener noreferrer" href="https://gcms.labrulez.com/bazaar"><strong>LabRulezGCMS Marketplace</strong></a></h4><p><strong>Spare parts for GC/MS:</strong></p><ul><li><a target="_blank" rel="noopener noreferrer" href="https://gcms.labrulez.com/bazaar?category=367&amp;search=turbo"><strong>Turbo pumps for GC/MS</strong></a></li></ul><p><strong>Spare parts for GC:</strong></p><ul><li><a target="_blank" rel="noopener noreferrer" href="https://gcms.labrulez.com/bazaar?category=366&amp;search=EPC"><strong>EPC modules</strong></a></li></ul><p><strong>Sample injection</strong></p><ul><li><a target="_blank" rel="noopener noreferrer" href="https://gcms.labrulez.com/bazaar/432"><strong>Autosampler 7693A for GC</strong></a></li></ul><p><strong>Consumables:</strong></p><ul><li><a target="_blank" rel="noopener noreferrer" href="https://gcms.labrulez.com/bazaar?category=7"><strong>GC columns </strong></a>and selected accessories</li></ul>

LabRulez at analytica 2026

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Eurachem is a European network promoting international traceability of chemical measurements and good analytical practice. It provides expert guidance, supports method validation, uncertainty evaluation, proficiency testing, and facilitates discussion on technical and policy issues in analytical quality.

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KNAUER AppliChrom® ABOA RPpetide GPC Columns for RP

AppliChrom® Polymer based reversed phase (RP) columns to reversed phase (RP) separation with orthogonal selectivity to silica based columns.

<p class="cke-user_style-important"><strong>AppliChrom® Polymer based reversed phase (RP) columns</strong></p><h4><strong>Properties:</strong></h4><ul><li>Rigid, spherical styrenedivinylbenzene copolymer&nbsp;</li><li><strong>Particle sizes between:</strong> 3 to 30 μm</li><li><strong>Pore sizes of: </strong>100–1 000 Å</li><li>Reversed Phase (RP) separation with orthogonal selectivity to silica based columns</li><li>Improved chemical stability</li><li><strong>Stable between:</strong> pH 1.5–14</li><li><strong>Temperature stability: </strong>10–90 °C</li><li><strong>Stable towards pressures:</strong> up to 150 bar</li><li>Low backpressure with high plate counts, ideal for preparative applications</li><li>Low column bleed for low detector noise</li><li>Up and down scaling ensured beteween analytical RP-HPLC and preparative process chromatography</li><li><strong>Recommended eluents:</strong> &nbsp;H₂O/ACN or H₂O/MeOH with up to 1 % TFA, FA or NaOH additive</li></ul><h4><strong>Applications</strong></h4><ul><li>Polar peptides, proteins or vitamins, hydrophobic amines</li></ul><h3><strong>GPC &amp; SEC Columns</strong></h3><p><strong>GPC &amp; SEC systems offer solutions for various applications and laboratories</strong></p><p>Gel permeation chromatography (GPC) and size exclusion chromatography (SEC) separate analytes by size using special columns with a porous matrix. The basic separation principle is based on the fact that smaller molecules penetrate these pores more strongly and therefore move more slowly through the matrix than larger molecules. GPC/SEC is often used as gentle and powerful purification technique or for quality control in research and development. Typical target molecules are polymers, dissolved in organic solvents for GPC, and macro biomolecules like proteins, antibodies or polysaccharides, dissolved in aqueous eluents for SEC.</p><p>Separation is facilitated by selecting optimal particle and pore sizes. Therefore we offer a <strong>wide range of pore sizes</strong> (35, 100, 150, 250, 350, 500, 1000, 1500, 10E5, 10E6, 10E7 Å) and <strong>several particle sizes</strong> (5, 7, 10, 12, 20 µm) as a choice to customize for every application task.</p><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/KNAUER_Size_Exclusion_principle_2a432edcc1.webp" alt="KNAUER: Size Exclusion - principle" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_KNAUER_Size_Exclusion_principle_2a432edcc1.webp 228w," sizes="100vw" width="1024" height="700"></p><h3><strong>KNAUER AppliChrom® ABOA columns for aqueous SEC, organic GPC, and special applications</strong></h3><ul><li>AppliChrom® ABOA StyDiViBe Series GPC-columns for GPC analysis of organic molecules using THF, toluene, chloroform.</li><li>AppliChrom ABOA SuperOH-P Series for aqueous GPC/SEC-analysis of mainly neutral and anionic polymers like heparins, polysaccharides, PEO,<br>PEG,....</li><li>AppliChrom® ABOA DMSO-Phil-P Series for DMSO GPC/SEC analysis (humic substances, UF/MUF-glue, p-NIPA, many polycondensates, protein from pea or leguminosae,...)</li><li>AppliChrom® ABOA DMAc-Phil-P Series for GPC/ SEC analysis in DMAc, DMF, NMP. Good for many medium polar substances, polyoxazolines, lignin,...</li><li>AppliChrom® ABOA Cat-Phil-P Series for aqueous GPC/SEC-analysis of special aminic and polycationic polymers like p-DADMAc, PEI/ polyethylenimin, chitosan, copolymers with polyaminofunctions, but also useful for polyanions or neutral polymers like heparin or PEO/PEG, dextran or pullulan.</li><li><strong>Seperation Ranges: </strong>100–50 000 000 Da</li></ul><h4><strong>You decide between classical GPC/SEC columns with conventional mixed pore technology or AppliChrom® BPT technology:</strong></h4><p>Separate oligomers and polymers with the new GPC media line from AppliChrom® BPT synthesis technology for large range, high resolution separations with increased accuracy in calibration (patent pending).</p><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/KNAUER_Appli_Chrom_ABOA_columns_58d5d380f6.jpg" alt="KNAUER: AppliChrom® ABOA columns." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_KNAUER_Appli_Chrom_ABOA_columns_58d5d380f6.jpg 245w," sizes="100vw" width="1379" height="718"></p>

KNAUER AppliChrom® ABOA VivoSep SEC Columns

AppliChrom® SEC columns for water soluble (bio)polymers are multi hydrophilic layered SEC media for highest efficiency and low interaction.

<p class="cke-user_style-important"><strong>AppliChrom® SEC columns for water soluble (bio)polymers</strong></p><h4><strong>Properties:</strong></h4><ul><li>Multi hydrophilic layered SEC media for highest efficiency and low interaction</li><li><strong>Available seperation ranges:</strong> from small molecules up to 5 500 kDa</li><li><strong>Temperature stability: </strong>10-90 °C</li><li><strong>Stable between: </strong>pH 2–10</li><li><strong>Pressure stability:</strong> up to 200 bar</li><li>Simple adaptation from HPLC to SEC with stainless steel instruments</li><li>Versatile tool for accurate protein molecular weight or size determination</li><li>Low column bleed for low detector noise</li><li>Increased lifetime of SEC columns through combination of proprietary particle and packing technology</li><li><strong>Recommended eluents:</strong> H₂O, buffers</li></ul><h4><strong>Applications</strong></h4><ul><li>Water soluble proteins, humic substances</li></ul><h3><strong>GPC &amp; SEC Columns</strong></h3><p><strong>GPC &amp; SEC systems offer solutions for various applications and laboratories</strong></p><p>Gel permeation chromatography (GPC) and size exclusion chromatography (SEC) separate analytes by size using special columns with a porous matrix. The basic separation principle is based on the fact that smaller molecules penetrate these pores more strongly and therefore move more slowly through the matrix than larger molecules. GPC/SEC is often used as gentle and powerful purification technique or for quality control in research and development. Typical target molecules are polymers, dissolved in organic solvents for GPC, and macro biomolecules like proteins, antibodies or polysaccharides, dissolved in aqueous eluents for SEC.</p><p>Separation is facilitated by selecting optimal particle and pore sizes. Therefore we offer a <strong>wide range of pore sizes</strong> (35, 100, 150, 250, 350, 500, 1000, 1500, 10E5, 10E6, 10E7 Å) and <strong>several particle sizes</strong> (5, 7, 10, 12, 20 µm) as a choice to customize for every application task.</p><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/KNAUER_Size_Exclusion_principle_2a432edcc1.webp" alt="KNAUER: Size Exclusion - principle" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_KNAUER_Size_Exclusion_principle_2a432edcc1.webp 228w," sizes="100vw" width="1024" height="700"></p><h3><strong>KNAUER AppliChrom® ABOA columns for aqueous SEC, organic GPC, and special applications</strong></h3><ul><li>AppliChrom® ABOA StyDiViBe Series GPC-columns for GPC analysis of organic molecules using THF, toluene, chloroform.</li><li>AppliChrom ABOA SuperOH-P Series for aqueous GPC/SEC-analysis of mainly neutral and anionic polymers like heparins, polysaccharides, PEO,<br>PEG,....</li><li>AppliChrom® ABOA DMSO-Phil-P Series for DMSO GPC/SEC analysis (humic substances, UF/MUF-glue, p-NIPA, many polycondensates, protein from pea or leguminosae,...)</li><li>AppliChrom® ABOA DMAc-Phil-P Series for GPC/ SEC analysis in DMAc, DMF, NMP. Good for many medium polar substances, polyoxazolines, lignin,...</li><li>AppliChrom® ABOA Cat-Phil-P Series for aqueous GPC/SEC-analysis of special aminic and polycationic polymers like p-DADMAc, PEI/ polyethylenimin, chitosan, copolymers with polyaminofunctions, but also useful for polyanions or neutral polymers like heparin or PEO/PEG, dextran or pullulan.</li><li><strong>Seperation Ranges: </strong>100–50 000 000 Da</li></ul><h4><strong>You decide between classical GPC/SEC columns with conventional mixed pore technology or AppliChrom® BPT technology:</strong></h4><p>Separate oligomers and polymers with the new GPC media line from AppliChrom® BPT synthesis technology for large range, high resolution separations with increased accuracy in calibration (patent pending).</p><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/KNAUER_Appli_Chrom_ABOA_columns_58d5d380f6.jpg" alt="KNAUER: AppliChrom® ABOA columns." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_KNAUER_Appli_Chrom_ABOA_columns_58d5d380f6.jpg 245w," sizes="100vw" width="1379" height="718"></p>

KNAUER AppliChrom® ABOA Cat-Phil SEC Columns

Interaction-free pure GPC & SEC base material with focus on polycationic, polyelectrolytic or funktional amine analytes

<p class="cke-user_style-important"><strong>AppliChrom® SEC columns for neutral, anionic and additional cationic polymers</strong></p><h4><strong>Properties:</strong></h4><ul><li>Interaction-free pure GPC &amp; SEC base material with focus on polycationic, polyelectrolytic or funktional amine analytes</li><li>No salt addition is needed to surpress surface adsorption</li><li>No ion exclusion phenomena</li><li>Possible to be used in universal calibration &amp; accurate molecular weight determination even for small polycationic molecules</li><li><strong>Temperature stability between:</strong> 10–90 °C</li><li><strong>Stable with:</strong> pH 2–10</li><li><strong>Simple, reliable and robust GPC &amp; SEC calibration</strong> from dextrans, pullulans et al.</li><li><strong>Low column bleed </strong>for low detector gain for improved refractive index, light scattering or viscosity detection</li><li><strong>Large volume</strong> and optimized mass transfer for polymers with optimized resolution</li><li><strong>Low cost due to long column lifetime</strong> - result of the combination of optimized particle and packing technology</li><li><strong>Recommended eluents:</strong> H₂O, buffers</li></ul><h4><strong>Applications</strong></h4><p><strong>Water soluble analytes like:</strong></p><ul><li>polycations, polyamines (chitosanes), polyethylenoxides, polysaccharides, polyanions (heparins, pectins), polyethylene oxide (PEO), polyethylene glycol (PEG), Ppolyethyleneimins (PEI), polyvinylimidazole</li></ul><h3><strong>GPC &amp; SEC Columns</strong></h3><p><strong>GPC &amp; SEC systems offer solutions for various applications and laboratories</strong></p><p>Gel permeation chromatography (GPC) and size exclusion chromatography (SEC) separate analytes by size using special columns with a porous matrix. The basic separation principle is based on the fact that smaller molecules penetrate these pores more strongly and therefore move more slowly through the matrix than larger molecules. GPC/SEC is often used as gentle and powerful purification technique or for quality control in research and development. Typical target molecules are polymers, dissolved in organic solvents for GPC, and macro biomolecules like proteins, antibodies or polysaccharides, dissolved in aqueous eluents for SEC.</p><p>Separation is facilitated by selecting optimal particle and pore sizes. Therefore we offer a <strong>wide range of pore sizes</strong> (35, 100, 150, 250, 350, 500, 1000, 1500, 10E5, 10E6, 10E7 Å) and <strong>several particle sizes</strong> (5, 7, 10, 12, 20 µm) as a choice to customize for every application task.</p><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/KNAUER_Size_Exclusion_principle_2a432edcc1.webp" alt="KNAUER: Size Exclusion - principle" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_KNAUER_Size_Exclusion_principle_2a432edcc1.webp 228w," sizes="100vw" width="1024" height="700"></p><h3><strong>KNAUER AppliChrom® ABOA columns for aqueous SEC, organic GPC, and special applications</strong></h3><ul><li>AppliChrom® ABOA StyDiViBe Series GPC-columns for GPC analysis of organic molecules using THF, toluene, chloroform.</li><li>AppliChrom ABOA SuperOH-P Series for aqueous GPC/SEC-analysis of mainly neutral and anionic polymers like heparins, polysaccharides, PEO,<br>PEG,....</li><li>AppliChrom® ABOA DMSO-Phil-P Series for DMSO GPC/SEC analysis (humic substances, UF/MUF-glue, p-NIPA, many polycondensates, protein from pea or leguminosae,...)</li><li>AppliChrom® ABOA DMAc-Phil-P Series for GPC/ SEC analysis in DMAc, DMF, NMP. Good for many medium polar substances, polyoxazolines, lignin,...</li><li>AppliChrom® ABOA Cat-Phil-P Series for aqueous GPC/SEC-analysis of special aminic and polycationic polymers like p-DADMAc, PEI/ polyethylenimin, chitosan, copolymers with polyaminofunctions, but also useful for polyanions or neutral polymers like heparin or PEO/PEG, dextran or pullulan.</li><li><strong>Seperation Ranges: </strong>100–50 000 000 Da</li></ul><h4><strong>You decide between classical GPC/SEC columns with conventional mixed pore technology or AppliChrom® BPT technology:</strong></h4><p>Separate oligomers and polymers with the new GPC media line from AppliChrom® BPT synthesis technology for large range, high resolution separations with increased accuracy in calibration (patent pending).</p><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/KNAUER_Appli_Chrom_ABOA_columns_58d5d380f6.jpg" alt="KNAUER: AppliChrom® ABOA columns." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_KNAUER_Appli_Chrom_ABOA_columns_58d5d380f6.jpg 245w," sizes="100vw" width="1379" height="718"></p>

KNAUER AppliChrom® ABOA StyDiViBe GPC Columns

Organic polystyrene-divinylbenzene copolymer base material with high porosity for high resolution

<p class="cke-user_style-important"><strong>AppliChrom® GPC columns for GPC analysis of organic molecules with THF, toluene, chloroform</strong></p><h4><strong>Properties:</strong></h4><ul><li>Organic polystyrene-divinylbenzene copolymer base material with high porosity for high resolution</li><li><strong>High pressure stability: </strong>up to 150 bar</li><li><strong>Temperature stable:</strong> between 10-145 °C</li><li><strong>GPC range:</strong> from 0.1 up to 50 000 kDa</li><li><strong>Low column bleed</strong> for low detector noise</li><li><strong>High purity</strong> to eliminate adsorptive effects and combine pure GPC with long lifetime</li><li><strong>Large calibration ranges</strong> for several decades due to the unique process of BPT technology</li><li>Range of individual porosities that can be used individually or in combination</li><li><strong>Optimized for: </strong>1 ml/min flow rate for 8 mm ID columns</li><li><strong>High level of reproducibility</strong> and peak precision due to the advanced packing procedure</li><li><strong>Recommended eluents:</strong> THF, Toluene, CHCl₃, dichlorbenzene, hexane/EtOAc (10:90, V:V)</li></ul><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/KNAUER_Appli_Chrom_ABOA_Sty_Di_Vi_Be_P_Molecular_sizes_range_ca9a682d2e.jpg" alt="KNAUER: AppliChrom® ABOA StyDiViBe-P - Molecular sizes range" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_KNAUER_Appli_Chrom_ABOA_Sty_Di_Vi_Be_P_Molecular_sizes_range_ca9a682d2e.jpg 235w," sizes="100vw" width="1072" height="712"></p><h4><strong>Applications</strong></h4><p><strong>Hydrophobic oligomers or polymers like:</strong></p><ul><li>PVC, PMMA, polystyrene, epoxy resins, polyesters, bisphenol A resins</li></ul><h3><strong>GPC &amp; SEC Columns</strong></h3><p><strong>GPC &amp; SEC systems offer solutions for various applications and laboratories</strong></p><p>Gel permeation chromatography (GPC) and size exclusion chromatography (SEC) separate analytes by size using special columns with a porous matrix. The basic separation principle is based on the fact that smaller molecules penetrate these pores more strongly and therefore move more slowly through the matrix than larger molecules. GPC/SEC is often used as gentle and powerful purification technique or for quality control in research and development. Typical target molecules are polymers, dissolved in organic solvents for GPC, and macro biomolecules like proteins, antibodies or polysaccharides, dissolved in aqueous eluents for SEC.</p><p>Separation is facilitated by selecting optimal particle and pore sizes. Therefore we offer a <strong>wide range of pore sizes</strong> (35, 100, 150, 250, 350, 500, 1000, 1500, 10E5, 10E6, 10E7 Å) and <strong>several particle sizes</strong> (5, 7, 10, 12, 20 µm) as a choice to customize for every application task.</p><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/KNAUER_Size_Exclusion_principle_2a432edcc1.webp" alt="KNAUER: Size Exclusion - principle" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_KNAUER_Size_Exclusion_principle_2a432edcc1.webp 228w," sizes="100vw" width="1024" height="700"></p><h3><strong>KNAUER AppliChrom® ABOA columns for aqueous SEC, organic GPC, and special applications</strong></h3><ul><li>AppliChrom® ABOA StyDiViBe Series GPC-columns for GPC analysis of organic molecules using THF, toluene, chloroform.</li><li>AppliChrom ABOA SuperOH-P Series for aqueous GPC/SEC-analysis of mainly neutral and anionic polymers like heparins, polysaccharides, PEO,<br>PEG,....</li><li>AppliChrom® ABOA DMSO-Phil-P Series for DMSO GPC/SEC analysis (humic substances, UF/MUF-glue, p-NIPA, many polycondensates, protein from pea or leguminosae,...)</li><li>AppliChrom® ABOA DMAc-Phil-P Series for GPC/ SEC analysis in DMAc, DMF, NMP. Good for many medium polar substances, polyoxazolines, lignin,...</li><li>AppliChrom® ABOA Cat-Phil-P Series for aqueous GPC/SEC-analysis of special aminic and polycationic polymers like p-DADMAc, PEI/ polyethylenimin, chitosan, copolymers with polyaminofunctions, but also useful for polyanions or neutral polymers like heparin or PEO/PEG, dextran or pullulan.</li><li><strong>Seperation Ranges: </strong>100–50 000 000 Da</li></ul><h4><strong>You decide between classical GPC/SEC columns with conventional mixed pore technology or AppliChrom® BPT technology:</strong></h4><p>Separate oligomers and polymers with the new GPC media line from AppliChrom® BPT synthesis technology for large range, high resolution separations with increased accuracy in calibration (patent pending).</p><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/KNAUER_Appli_Chrom_ABOA_columns_58d5d380f6.jpg" alt="KNAUER: AppliChrom® ABOA columns." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_KNAUER_Appli_Chrom_ABOA_columns_58d5d380f6.jpg 245w," sizes="100vw" width="1379" height="718"></p>

KNAUER AppliChrom® ABOA DMAc-Phil GPC Columns

Organic polymer base material with high porosity for high resolution

<p class="cke-user_style-important"><strong>AppliChrom® GPC columns for organic GPC analyses in DMAc or DMF</strong></p><h4><strong>Properties:</strong></h4><ul><li>Organic polymer base material with high porosity for high resolution</li><li>Wide range of porosities and particle sizes</li><li><strong>Pressure stability:</strong> up to 150 bar</li><li><strong>Stable between: </strong>35-145 °C</li><li><strong>Excellent reproducibility</strong> and resolution due to the advanced packing procedure</li><li><strong>High purity</strong> to eliminate adsorptive effects and combine pure GPC with long lifetime</li><li><strong>GPC range from:</strong> 100-1 000 000 Da</li><li><strong>Optimal for: </strong>0.5 ml/min flow rates with 8 mm ID colums</li><li><strong>Recommended eluents: </strong>DMAc, DMF, NMP, LiBr additive</li></ul><h4><strong>Applications</strong></h4><p><strong>Slightly polar analytes like:</strong></p><ul><li>PMMA, polyacryl nitril (PAN), acrylnitrile butadiene styrene (ABS), cellulose, and all DMF and DMAc soluble polymers</li></ul><h3><strong>GPC &amp; SEC Columns</strong></h3><p><strong>GPC &amp; SEC systems offer solutions for various applications and laboratories</strong></p><p>Gel permeation chromatography (GPC) and size exclusion chromatography (SEC) separate analytes by size using special columns with a porous matrix. The basic separation principle is based on the fact that smaller molecules penetrate these pores more strongly and therefore move more slowly through the matrix than larger molecules. GPC/SEC is often used as gentle and powerful purification technique or for quality control in research and development. Typical target molecules are polymers, dissolved in organic solvents for GPC, and macro biomolecules like proteins, antibodies or polysaccharides, dissolved in aqueous eluents for SEC.</p><p>Separation is facilitated by selecting optimal particle and pore sizes. Therefore we offer a <strong>wide range of pore sizes</strong> (35, 100, 150, 250, 350, 500, 1000, 1500, 10E5, 10E6, 10E7 Å) and <strong>several particle sizes</strong> (5, 7, 10, 12, 20 µm) as a choice to customize for every application task.</p><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/KNAUER_Size_Exclusion_principle_2a432edcc1.webp" alt="KNAUER: Size Exclusion - principle" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_KNAUER_Size_Exclusion_principle_2a432edcc1.webp 228w," sizes="100vw" width="1024" height="700"></p><h3><strong>KNAUER AppliChrom® ABOA columns for aqueous SEC, organic GPC, and special applications</strong></h3><ul><li>AppliChrom® ABOA StyDiViBe Series GPC-columns for GPC analysis of organic molecules using THF, toluene, chloroform.</li><li>AppliChrom ABOA SuperOH-P Series for aqueous GPC/SEC-analysis of mainly neutral and anionic polymers like heparins, polysaccharides, PEO,<br>PEG,....</li><li>AppliChrom® ABOA DMSO-Phil-P Series for DMSO GPC/SEC analysis (humic substances, UF/MUF-glue, p-NIPA, many polycondensates, protein from pea or leguminosae,...)</li><li>AppliChrom® ABOA DMAc-Phil-P Series for GPC/ SEC analysis in DMAc, DMF, NMP. Good for many medium polar substances, polyoxazolines, lignin,...</li><li>AppliChrom® ABOA Cat-Phil-P Series for aqueous GPC/SEC-analysis of special aminic and polycationic polymers like p-DADMAc, PEI/ polyethylenimin, chitosan, copolymers with polyaminofunctions, but also useful for polyanions or neutral polymers like heparin or PEO/PEG, dextran or pullulan.</li><li><strong>Seperation Ranges: </strong>100–50 000 000 Da</li></ul><h4><strong>You decide between classical GPC/SEC columns with conventional mixed pore technology or AppliChrom® BPT technology:</strong></h4><p>Separate oligomers and polymers with the new GPC media line from AppliChrom® BPT synthesis technology for large range, high resolution separations with increased accuracy in calibration (patent pending).</p><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/KNAUER_Appli_Chrom_ABOA_columns_58d5d380f6.jpg" alt="KNAUER: AppliChrom® ABOA columns." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_KNAUER_Appli_Chrom_ABOA_columns_58d5d380f6.jpg 245w," sizes="100vw" width="1379" height="718"></p>

KNAUER AppliChrom® ABOA DMSO-Phil GPC Columns

AppliChrom® DMSO-PHIL optimized for DMSO-GPC applications from an interaction-free pure GPC base material

<p class="cke-user_style-important"><strong>AppliChrom® DMSO-PHIL optimized for DMSO-GPC applications</strong></p><h4><strong>Properties:</strong></h4><ul><li>Interaction-free pure GPC base material</li><li><strong>Temperature stabel between:</strong> 40-90 °C (145 °C) depending on application</li><li><strong>Simple, reliable and robust </strong>GPC &amp; SEC calibration from dextrans, pullulans et al.</li><li><strong>Low column bleed</strong> for low detector gain for improved refractive index, light scattering or viscosity detection</li><li><strong>12 µm particle technology</strong> for low back pressure</li><li>Tolerates traces of water and NaNO₃ additve</li><li><strong>Large volume and optimized mass transfer</strong> for polymers with optimized resolution</li><li><strong>Low cost due to long column lifetime</strong> - result of the combination of optimized particle and packing technology</li><li><strong>Recommended eluents:</strong> DMSO, 0.075 M NaNO₃</li></ul><h4><strong>Applications</strong></h4><p><strong>Polar, not water soluble oligomers or polymers like:</strong></p><ul><li>polysaccharides, lignin, amylose, starch, humic acids, polyvenyl pyridin, PNIPA, polyvinyl alcohol (PVA), melamin-urea-formaldehyd resin (MUF) or urea-formaldehyd resin (UF)</li></ul><h3><strong>GPC &amp; SEC Columns</strong></h3><p><strong>GPC &amp; SEC systems offer solutions for various applications and laboratories</strong></p><p>Gel permeation chromatography (GPC) and size exclusion chromatography (SEC) separate analytes by size using special columns with a porous matrix. The basic separation principle is based on the fact that smaller molecules penetrate these pores more strongly and therefore move more slowly through the matrix than larger molecules. GPC/SEC is often used as gentle and powerful purification technique or for quality control in research and development. Typical target molecules are polymers, dissolved in organic solvents for GPC, and macro biomolecules like proteins, antibodies or polysaccharides, dissolved in aqueous eluents for SEC.</p><p>Separation is facilitated by selecting optimal particle and pore sizes. Therefore we offer a <strong>wide range of pore sizes</strong> (35, 100, 150, 250, 350, 500, 1000, 1500, 10E5, 10E6, 10E7 Å) and <strong>several particle sizes</strong> (5, 7, 10, 12, 20 µm) as a choice to customize for every application task.</p><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/KNAUER_Size_Exclusion_principle_2a432edcc1.webp" alt="KNAUER: Size Exclusion - principle" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_KNAUER_Size_Exclusion_principle_2a432edcc1.webp 228w," sizes="100vw" width="1024" height="700"></p><h3><strong>KNAUER AppliChrom® ABOA columns for aqueous SEC, organic GPC, and special applications</strong></h3><ul><li>AppliChrom® ABOA StyDiViBe Series GPC-columns for GPC analysis of organic molecules using THF, toluene, chloroform.</li><li>AppliChrom ABOA SuperOH-P Series for aqueous GPC/SEC-analysis of mainly neutral and anionic polymers like heparins, polysaccharides, PEO,<br>PEG,....</li><li>AppliChrom® ABOA DMSO-Phil-P Series for DMSO GPC/SEC analysis (humic substances, UF/MUF-glue, p-NIPA, many polycondensates, protein from pea or leguminosae,...)</li><li>AppliChrom® ABOA DMAc-Phil-P Series for GPC/ SEC analysis in DMAc, DMF, NMP. Good for many medium polar substances, polyoxazolines, lignin,...</li><li>AppliChrom® ABOA Cat-Phil-P Series for aqueous GPC/SEC-analysis of special aminic and polycationic polymers like p-DADMAc, PEI/ polyethylenimin, chitosan, copolymers with polyaminofunctions, but also useful for polyanions or neutral polymers like heparin or PEO/PEG, dextran or pullulan.</li><li><strong>Seperation Ranges: </strong>100–50 000 000 Da</li></ul><h4><strong>You decide between classical GPC/SEC columns with conventional mixed pore technology or AppliChrom® BPT technology:</strong></h4><p>Separate oligomers and polymers with the new GPC media line from AppliChrom® BPT synthesis technology for large range, high resolution separations with increased accuracy in calibration (patent pending).</p><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/KNAUER_Appli_Chrom_ABOA_columns_58d5d380f6.jpg" alt="KNAUER: AppliChrom® ABOA columns." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_KNAUER_Appli_Chrom_ABOA_columns_58d5d380f6.jpg 245w," sizes="100vw" width="1379" height="718"></p>

KNAUER AppliChrom® ABOA HFIP-Phil GPC Columns

AppliChrom® GPC columns for separations in HFIP with broad pore technology for GPC for analytes with challenging solution behavior

<p class="cke-user_style-important"><strong>AppliChrom® GPC columns for separations in HFIP</strong></p><h4><strong>Properties:</strong></h4><ul><li>Broad pore technology for GPC for analytes with challenging solution behavior</li><li><strong>Separation range beween:</strong> 0.1–50 000 kDa</li><li>Range of individual porosities that can be used individually or in combination</li><li><strong>Temperature stable between:</strong> 35–145 °C</li><li><strong>Long column lifetime</strong> - result of the combination of optimized particle and packing technology</li><li><strong>Low column bleed</strong> for low detector noise</li><li><strong>Excellent reproducibility and resolution</strong> due to the advanced packing procedure</li><li><strong>Calibration standard:</strong> PMMA or PEO &nbsp;</li><li><strong>Recommended eluents</strong>: Hexafluoroisopropanol (HFIP), 0.1 % KTFa as modifier</li></ul><h4><strong>Applications</strong></h4><p><strong>Crystalline highperformance polymers like:&nbsp;</strong></p><ul><li>polyamides (PA), polyethylen terepthalate (PET), polybutylen terepthalate (PBT), polylactic acid (PLA), polyamid 6 (PA6), polycaprolactam, paraformaldehyde, polyoxymethylene (POM), polyacetal, several polyoxazolines</li></ul><h3><strong>GPC &amp; SEC Columns</strong></h3><p><strong>GPC &amp; SEC systems offer solutions for various applications and laboratories</strong></p><p>Gel permeation chromatography (GPC) and size exclusion chromatography (SEC) separate analytes by size using special columns with a porous matrix. The basic separation principle is based on the fact that smaller molecules penetrate these pores more strongly and therefore move more slowly through the matrix than larger molecules. GPC/SEC is often used as gentle and powerful purification technique or for quality control in research and development. Typical target molecules are polymers, dissolved in organic solvents for GPC, and macro biomolecules like proteins, antibodies or polysaccharides, dissolved in aqueous eluents for SEC.</p><p>Separation is facilitated by selecting optimal particle and pore sizes. Therefore we offer a <strong>wide range of pore sizes</strong> (35, 100, 150, 250, 350, 500, 1000, 1500, 10E5, 10E6, 10E7 Å) and <strong>several particle sizes</strong> (5, 7, 10, 12, 20 µm) as a choice to customize for every application task.</p><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/KNAUER_Size_Exclusion_principle_2a432edcc1.webp" alt="KNAUER: Size Exclusion - principle" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_KNAUER_Size_Exclusion_principle_2a432edcc1.webp 228w," sizes="100vw" width="1024" height="700"></p><h3><strong>KNAUER AppliChrom® ABOA columns for aqueous SEC, organic GPC, and special applications</strong></h3><ul><li>AppliChrom® ABOA StyDiViBe Series GPC-columns for GPC analysis of organic molecules using THF, toluene, chloroform.</li><li>AppliChrom ABOA SuperOH-P Series for aqueous GPC/SEC-analysis of mainly neutral and anionic polymers like heparins, polysaccharides, PEO,<br>PEG,....</li><li>AppliChrom® ABOA DMSO-Phil-P Series for DMSO GPC/SEC analysis (humic substances, UF/MUF-glue, p-NIPA, many polycondensates, protein from pea or leguminosae,...)</li><li>AppliChrom® ABOA DMAc-Phil-P Series for GPC/ SEC analysis in DMAc, DMF, NMP. Good for many medium polar substances, polyoxazolines, lignin,...</li><li>AppliChrom® ABOA Cat-Phil-P Series for aqueous GPC/SEC-analysis of special aminic and polycationic polymers like p-DADMAc, PEI/ polyethylenimin, chitosan, copolymers with polyaminofunctions, but also useful for polyanions or neutral polymers like heparin or PEO/PEG, dextran or pullulan.</li><li><strong>Seperation Ranges: </strong>100–50 000 000 Da</li></ul><h4><strong>You decide between classical GPC/SEC columns with conventional mixed pore technology or AppliChrom® BPT technology:</strong></h4><p>Separate oligomers and polymers with the new GPC media line from AppliChrom® BPT synthesis technology for large range, high resolution separations with increased accuracy in calibration (patent pending).</p><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/KNAUER_Appli_Chrom_ABOA_columns_58d5d380f6.jpg" alt="KNAUER: AppliChrom® ABOA columns." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_KNAUER_Appli_Chrom_ABOA_columns_58d5d380f6.jpg 245w," sizes="100vw" width="1379" height="718"></p>

Waters ACQUITY Advanced Polymer Chromatography (APC) System

The ACQUITY APC System Increase sample throughput and laboratory operations by obtaining accurate and repeatable sample separation up to 20 time faster than before.

<div><div><h4><strong>The highest performance for polymers and oligomers</strong></h4></div></div><div><div><p><strong>Today’s polymer scientists operate within an increasingly dynamic market space, one with a growing demand for high-performing materials, bio-material innovations, and intense competition. But the long run times of traditional chromatography polymer characterization has made keeping up with this demand a challenge for many labs.</strong></p><p>Not anymore. With the <strong>next era in polymer separation</strong>, the <strong>ACQUITY Advanced Polymer Chromatography (APC) System</strong>, you can achieve the <strong>highest resolution size-based separations</strong> for <strong>chromatographic polymer and oligomer characterization</strong>, all <strong>faster than ever</strong>. With the <strong>ACQUITY APC System</strong>, you can <strong>improve data consistency and quality</strong> with <strong>fast, daily calibration</strong>, and <strong>automate your method development</strong> with <strong>advanced system technologies</strong> – so you can <strong>innovate faster</strong> while <strong>improving lab operations.</strong></p><div><div><div><div><h3><strong>Features and Benefits</strong></h3><ul><li><strong>Accelerate your insights</strong> - Reduce laboratory turnaround times for polymer samples by getting answers to your development labs, your manufacturing operations teams, and your customers 5 to 20 times faster than traditional SEC/GPC with the ultra-low system dispersion of the ACQUITY APC System paired with <a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/products/1950"><strong>ACQUITY APC Columns</strong></a>.</li><li><strong>Get the accurate data you need for informed decisions</strong> - Gain more insight into your polymer samples through compatibility with a wide range of specific detection techniques (refractive index, UV/Vis, PDA, ELS) and third-party advanced detectors like multi-angle light scattering and viscometry. Get accurate molecular weight (MW) data every day with the precise flow delivery of the isocratic solvent manager.</li><li><strong>Optimize method development, even in harsh conditions</strong> - Optimize method development via rapid and automated solvent switching and aggressive solvent compatibility, which supports the harsh separation conditions associated with polymer analyses.</li><li><strong>Improve lab efficiency with one system</strong> - Increase analytical scope and laboratory efficiency by leveraging one system to perform multiple applications, including basic liquid chromatography (LC), gradient, isocratic, reverse-phase, and gel permeation chromatography (GPC).</li><li><strong>Simplify your column management</strong> - Simplify and optimize column bank calibration through standards that are matched to column bank MW ranges. Versatile column management allows you to automatically select up to two banks of <strong>ACQUITY APC</strong> columns and up to two banks of traditional GPC columns - all housed in a stable, temperature-controlled environment.</li></ul></div></div><div><div><h4><strong>Recommended Use</strong></h4><p>Highest-resolution, size-based separations for chromatographic polymer and oligomer characterization.</p><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Waters_ACQUITY_APC_System_nakonfigurovany_pro_automaticke_prepinani_jak_APC_tak_GPC_kolon_e198c774f4/Waters-ACQUITY-APC-System-nakonfigurovany-pro-automaticke-prepinani-jak-APC-tak-GPC-kolon.jpg" alt="Waters ACQUITY Advanced Polymer Chromatography (APC) System." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Waters_ACQUITY_APC_System_nakonfigurovany_pro_automaticke_prepinani_jak_APC_tak_GPC_kolon_e198c774f4/thumbnail_Waters-ACQUITY-APC-System-nakonfigurovany-pro-automaticke-prepinani-jak-APC-tak-GPC-kolon.jpg 213w," sizes="100vw" width="969" height="710"></p><div><div><h3><strong>Accelerate innovation with enhanced insight</strong></h3><p>Harnessing the benefits of advanced polymer chromatography with the ACQUITY APC System means obtaining accurate and repeatable polymer and oligomer molecular weight information anywhere between<strong> 5 to 20 times faster than was possible</strong> with traditional<strong> GPC/SEC technology</strong> - so you can innovate faster while improving laboratory operations.</p><p><strong>With the ACQUITY APC System, you can:</strong></p><ul><li><strong>Reduce laboratory turnaround times for polymer samples&nbsp;</strong>by getting answers to your development labs, your manufacturing operations teams, and your customers - faster.</li><li><strong>Drive innovation</strong>&nbsp;with faster results and more information so that your entire operation can respond more rapidly by decreasing development cycle times and accelerating time to market.</li><li><strong>Streamline process monitoring</strong>&nbsp;and achieve better batch control with the agility to make timely decisions “on-the-fly” about process and synthesis optimization<strong>.</strong></li><li><strong>Significantly reduce the analysis cost per sample</strong>&nbsp;by reducing solvent consumption and waste disposal volumes.</li></ul><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Waters_ACQUITY_Advanced_Polymer_Chromatography_APC_System_ca5154865a.jpg" alt="Waters ACQUITY Advanced Polymer Chromatography (APC) System: Accelerate your innovation with enhanced insight into polymer chemical structure with the ACQUITY APC System." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Waters_ACQUITY_Advanced_Polymer_Chromatography_APC_System_ca5154865a.jpg 245w," sizes="100vw" width="1024" height="600"></p><div><div><h3><strong>Optimize method development with solvent switching and compatibility</strong></h3><p>Using the ACQUITY APC System with <strong>Polymer Quaternary Solvent Manager</strong> (p-QSM) <strong>technology </strong>provides chemists and polymer scientists with the ultimate flexibility to analyze the most complex polymer blends and additives using standard polymer chromatography, gradient polymer elution chromatography (GPEC), and reverse-phase LC with a single system.</p><p>Additional system features allow automated solvent selection of up to six different solvents. Automated column bank switching capabilities can also be paired with the rigid and solvent-resilient particles of the <a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/products/1950"><strong>ACQUITY APC Columns</strong></a> to achieve the world’s first truly automated solution for method development of size-based polymer separations. This supports separation of polymers in hours, instead of days.</p><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Waters_ACQUITY_Advanced_Polymer_Chromatography_APC_System_2_b95f96f0a2.jpg" alt="Waters ACQUITY Advanced Polymer Chromatography (APC) System: Repeatable analysis of poly (methyl methacrylate co ethylacrylate) using the same two running ACQUITY APC 4.6 x 150 mm Columns (450 Å and 125 Å) in series after automated switching of the solvents from THF to toluene to DMF and back to THF." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Waters_ACQUITY_Advanced_Polymer_Chromatography_APC_System_2_b95f96f0a2.jpg 245w," sizes="100vw" width="1024" height="609"></p></div></div><div><div><h3><strong>Use advanced detection for even more insights</strong></h3><p>Get the valuable decision-making information you need from a single analysis by equipping the ACQUITY APC System with advanced detectors. Combining the ACQUITY APC System with advanced detection solutions significantly increases information attainable from <strong>size-exclusion chromatography</strong> (SEC) analysis through addition of <strong>refractive index</strong> (RI), <strong>ultraviolet </strong>(UV),<strong> photodiode array </strong>(PDA), <strong>light scattering</strong> (LS), and <strong>differential viscometry</strong> (IV). Advanced detection options from <strong>Wyatt Technology</strong> are seamlessly integrated with ACQUITY APC System, enabling you to fully characterize samples to better inform the structure-property relationship of novel complex polymers.</p></div></div><div><div><h3><strong>Keep pace with your research with dedicated consumables</strong></h3><p>The ACQUITY APC System is even more powerful with <a target="_blank" rel="noopener noreferrer" href="https://www.waters.com/nextgen/global/products/columns/acquity-apc-columns.html"><strong>ACQUITY APC Columns</strong></a> and <a target="_blank" rel="noopener noreferrer" href="https://www.waters.com/nextgen/global/products/standards-and-reagents/acquity-advanced-polymer-chromatography-calibration-standards-ki.html"><strong>ACQUITY APC Calibration Standards Kits</strong></a>. The Ethylene Bridged Hybrid (BEH) Technology in ACQUITY APC Columns creates particles that ensure extreme column performance and long column lifetime under harsh operating conditions – ideal for general purpose, reversed-phase chromatography, and for method development due to extreme pH stability and applicability to the broadest range of compound classes.</p><p>With run times of less than 10 minutes, a bank of ACQUITY APC Columns can be <strong>calibrated in less than 30 minutes</strong> using ACQUITY APC Calibration Standards Kits.&nbsp;These standard kits have been matched to the MW range of ACQUITY APC Columns, generating a 10-point calibration for any bank combination in a simple dilute-and-shoot format. With daily column bank calibration, you can greatly improve data consistency to increase confidence in batch-to-batch measurements. Select the best column and calibration standards for a given application with our convenient <a target="_blank" rel="noopener noreferrer" href="https://www.waters.com/waters/promotionDetail.htm?id=134823417">selector tool</a>.&nbsp;</p><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Waters_ACQUITY_Advanced_Polymer_Chromatography_APC_System_4_056bb6d284.jpg" alt="Waters ACQUITY Advanced Polymer Chromatography (APC) System: Ethylene Bridged Hybrid (BEH) Technology helps you keep up with the pace of your research with state-of-the-art reversed-phase and HILIC HPLC columns." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Waters_ACQUITY_Advanced_Polymer_Chromatography_APC_System_4_056bb6d284.jpg 245w," sizes="100vw" width="1024" height="620"><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Waters_ACQUITY_Advanced_Polymer_Chromatography_APC_System_5_7a814e249b.jpg" alt="Waters ACQUITY Advanced Polymer Chromatography (APC) System: Improve data and consistency with ACQUITY APC Calibration Standards." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Waters_ACQUITY_Advanced_Polymer_Chromatography_APC_System_5_7a814e249b.jpg 245w," sizes="100vw" width="1024" height="591"></p></div></div></div></div></div></div></div></div></div></div>

Thermo Scientific SurePac Bio 550 SEC MDi HPLC Columns

SurePac™ Bio 550 SEC MDi™ column delivers high-resolution, reproducible AAV size-based separations with 3 μm silica, 550 Å pores, and bioinert hardware for optimal recovery and QC reliability.

<p><strong>Experience the future of Size-Exclusion Chromatography (SEC) with SurePac™ Bio 550 SEC MDi™ analytical columns and guards exclusively designed to deliver robust and reproducible performance at high resolution and throughput to accelerate your therapeutics analysis process. The column's groundbreaking MDi™ technology integrates our advanced 3 μm monodisperse silica particle platform with bioinert hardware to ensure superior reliability for best sample recovery. SurePac™ Bio 550 SEC MDi™ column is ideal for AAV gene therapeutics analysis and offers excellent selectivity due to the dedicated smaller particle (3 μm) size combined with wide-pore (550Å) for separating monomeric AAVs from aggregates based on their size differences.</strong></p><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Thermo_Scientific_Sure_Pac_Bio_550_SEC_M_Di_HPLC_Columns_3_8391a584e0.jpg" alt="Thermo Scientific: SEM image of 3μm monodisperse silica particles (left) vs. 3 μm polydisperse particles (right)" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Thermo_Scientific_Sure_Pac_Bio_550_SEC_M_Di_HPLC_Columns_3_8391a584e0.jpg 245w," sizes="100vw" width="1023" height="508"></p><p>SurePac Bio 550 SEC MDi columns and guards are based on high-purity, spherical, porous (550 Å), 3 μm monodispersed silica particles that are covalently modified with a proprietary diol hydrophilic layer. This unique design yields shorter diffusion distances and enhanced reproducible mass transfer and narrower peaks. The consistent size of these particles facilitates precise control over media synthesis and column packing, significantly improving column-to-column and lot-to-lot reproducibility. The columns are housed in a hydrophilic bioinert-coated stainless-steel hardware to eliminate secondary interactions, ensuring optimal performance during the initial injection process.</p><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Thermo_Scientific_Sure_Pac_Bio_550_SEC_M_Di_HPLC_Columns_043f131056.jpg" alt="Thermo Scientific: SurePac Bio 550 SEC MDi HPLC Columns" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Thermo_Scientific_Sure_Pac_Bio_550_SEC_M_Di_HPLC_Columns_043f131056.jpg 156w," sizes="100vw" width="650" height="650"></p><ul><li>Ideal for AAV gene therapy characterization and quality control assessment due to selectivity of the dedicated smaller particle (3 μm) combined with wide-pore (550Å) size</li><li>Superior column-to-column and lot-to-lot reproducibility eliminates column variability as a concern in stability and QA/QC testing</li><li>High-efficiency peaks with excellent recovery</li><li>Higher resolution and throughput for fast turnaround time without sacrificing performance</li><li>Bioinert hardware to eliminate analyte adsorption</li></ul><h4><strong>Specifications</strong></h4><ul><li><strong>Max. Pressure: </strong>3500 psi (241 bar)</li><li><strong>Packing Material:</strong> Monodisperse Silica Resin</li><li><strong>Flow Rate: </strong>0.6 mL/min</li><li><strong>Temperature:</strong> 20°C to 60°C</li><li><strong>Phase:</strong> Size Exclusion</li><li><strong>Particle Size: </strong>3 μm</li><li><strong>pH: </strong>2 to 8</li></ul>

Waters SEC Columns

Get unmatched batch-to-batch reproducibility with the wide range of Waters Size-Exclusion Chromatography (SEC) Columns and standards for protein and peptide characterization.

<h4><strong>Size-based separations of peptides, proteins, nucleic acids, LNPs, and viral vectors</strong></h4><p>Get unmatched batch-to-batch reproducibility with the wide range of <strong>Waters Size-Exclusion Chromatography (SEC) Columns</strong> and standards for protein and peptide characterization.</p><p><strong>SEC</strong> is a powerful technique for aggregate and fragment analysis in the <strong>research, development</strong>, and <strong>manufacturing of biotherapeutic proteins</strong>, such as <strong>insulin</strong> and <strong>monoclonal antibodies</strong> (mAb). With SEC, also known as gel filtration, you can separate proteins based on their size (hydrodynamic radii versus absolute molecular weight) in solution, with larger sized species eluting before smaller proteins.</p><p>The <strong>pore size and volume</strong> of the SEC particles used in the packed column drives this <strong>isocratic based technique</strong>, so the column you choose matters. As the molecular weight of the proteins of interest increases, SEC columns with comparatively larger pore size particles are selected.</p><h4><strong>Overview</strong></h4><ul><li>Gain efficiency with this “platform based” method for <strong>proteins ranging from 10,000 to 650,000 Daltons</strong>, leveraging <strong>MaxPeak Premier Technology</strong> coupled with <strong>BEH-PEO Particle Technology&nbsp;</strong> &nbsp;&nbsp;</li><li><strong>Minimize non-desired secondary interactions </strong>for improved separation reproducibility and results with Ethylene Bridged Hybrid (BEH) SEC particles</li><li>Ensure reliable <strong>column-to-column and batch-to-batch performance</strong> with columns quality control tested with relevant peptide, protein, and mAb samples &nbsp;</li><li>Achieve reliable <strong>high throughput analysis on UPLC</strong> systems with our <strong>1.7 µm particle size</strong> offering and reproducible separations on any <strong>HPLC, UHPLC, or UPLC system</strong> to address different application needs with <strong>2.5 µm&nbsp;</strong></li><li><strong>Extend analytical column life</strong> and<strong> lower cost per analyses </strong>with SEC Guards&nbsp;</li><li>Confirm on-site SEC column and LC system performance with available BEH125, BEH200, BEH450, and mAb Size Variant Standards &nbsp;&nbsp;</li><li><strong>Manufactured in a cGMP, ISO 9001</strong> certified plant using stringent manufacturing protocols and ultra-pure reagents</li></ul><p><strong>Recommended Use: </strong>For size-based peptide, protein, and viral vector analyses.</p><h4><strong>System Dispersion Specifications for Column Recommendations</strong></h4><h5><strong>LC System Dispersions &lt;20 µL (UPLC)</strong></h5><ul><li>ACQUITY Premier Protein SEC 250 Å Guards and Columns</li><li>ACQUITY UPLC Protein BEH SEC 125 Å, 200 Å, and 450 Å Guards and Columns</li><li>1.7 µm guards and columns for low dispersion UPLC systems</li></ul><h5><strong>LC System Dispersions &gt;20 µL – ≤35 µL (UHPLC)</strong></h5><ul><li>XBridge Premier Protein SEC 250 Å Guards and Columns</li><li>XBridge Protein BEH SEC 125 Å, 200 Å, and 450 Å Guards and Columns</li><li>BioResolve mAb SEC Guards and Columns</li><li>2.5 µm guards and columns for UHPLC systems</li></ul><h5><strong>LC System Dispersions &gt;35 µL (HPLC)</strong></h5><ul><li>XBridge Protein BEH SEC 125 Å, 200 Å, and 450 Å Guards and Columns</li><li>XBridge Premier Protein SEC 250 Å Guards and Columns</li><li>BioResolve mAb SEC Guards and Columns</li><li>BioSuite SEC Guards and Columns</li><li>3.5 µm guards and columns for HPLC systems</li></ul><h4><strong>GTxResolve SEC Columns</strong></h4><h5><strong>GTxResolve Premier BEH SEC 450 Å Columns</strong></h5><ul><li>2.5 µm particle size columns for UHPLC and UPLC separations</li><li>Lower secondary interactions and lower noise suitable for platform methods</li><li>Low adsorption hardware necessary for analysis of biologics samples</li><li>450 Å pore size with up to 50% higher resolving power reducing need for sample consumption, resulting in faster analyses<ul><li><a target="_blank" rel="noopener noreferrer" href="https://www.waters.com/nextgen/us/en/products/columns/GTxResolve-sec-columns.html"><strong>Explore GTxResolve SEC Columns</strong></a></li></ul></li></ul><h5><strong>GTxResolve Premier SEC 1000 Å Columns</strong></h5><ul><li>3 µm columns for UHPLC and UPLC separations</li><li>1000 Å columns with high efficiency for aggregate analysis of GTx drugs with diameter of 80-200 nm</li><li>Low secondary interaction &amp; improved compatibility with universally applied detector, such as MALS</li><li>Low adsorption hardware necessary for analysis of biologics samples<ul><li><a target="_blank" rel="noopener noreferrer" href="https://www.waters.com/nextgen/us/en/products/columns/GTxResolve-sec-columns.html"><strong>Explore GTxResolve SEC Columns</strong></a></li></ul></li></ul><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Waters_G_Tx_Resolve_SEC_Columns_1b8741a62b.jpg" alt="Waters: GTxResolve SEC Columns" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Waters_G_Tx_Resolve_SEC_Columns_1b8741a62b.jpg 234w," sizes="100vw" width="1024" height="682"></p><h4><strong>Protein SEC Columns</strong></h4><h5><strong>ACQUITY Premier Protein SEC 250 Å Guards and Columns</strong></h5><ul><li>Sub-2-μm particle columns for UPLC separations</li><li>250 Å pore size</li><li>High throughput &amp; resolution SEC separations of biomolecules ranging from 10,000 to 650,000 Daltons on low dispersion UPLC Systems</li><li>Available SEC Guard Columns to extend analytical SEC Column life</li><li>Significantly reduce non-desired ionic and hydrophobic secondary interaction for desired SEC platform methods with MaxPeak Premier Technology and BEH-PEO SEC particle technology<ul><li><a target="_blank" rel="noopener noreferrer" href="https://www.waters.com/nextgen/us/en/products/columns/acquity-premier-columns.html"><strong>Explore ACQUITY Premier Protein BEH SEC Guards and Columns</strong></a></li></ul></li></ul><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Waters_ACQUITY_Premier_Columns_featuring_Max_Peak_HPS_Technology_11a541350e.jpg" alt="Waters: ACQUITY Premier Columns featuring MaxPeak HPS Technology" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Waters_ACQUITY_Premier_Columns_featuring_Max_Peak_HPS_Technology_11a541350e.jpg 234w," sizes="100vw" width="1024" height="682"></p><h5><strong>XBridge Premier Protein SEC 250 Å Guards and Columns</strong></h5><ul><li>2.5 μm particle columns for HPLC and UHPLC separations</li><li>250 Å pore size</li><li>High resolution SEC separations of biomolecules ranging from 10,000 to 650,000 Daltons on any LC system</li><li>Available SEC Guard Columns to extend analytical SEC Column life</li><li>Significantly reduce non-desired ionic and hydrophobic secondary interaction for desired SEC platform methods with MaxPeak Premier Technology and BEH-PEO SEC particle technology</li><li>Specifically designed and QC tested with NIST-based, mAb Size Variant Standard to help ensure batch-to-batch consistency<ul><li><a target="_blank" rel="noopener noreferrer" href="https://www.waters.com/waters/global/Waters-XBridge-Premier-Columns/nav.htm"><strong>Explore XBridge Premier Protein BEH SEC Columns</strong></a></li></ul></li></ul><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Waters_X_Bridge_Premier_Columns_0230c90278.jpg" alt="Waters: XBridge Premier Columns" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Waters_X_Bridge_Premier_Columns_0230c90278.jpg 193w," sizes="100vw" width="950" height="768"></p><h5><strong>BioResolve mAb SEC Guards and Columns</strong></h5><ul><li>2.5 μm particle columns for UPLC, UHPLC, and HPLC separations</li><li>200 Å pore size</li><li>Obtain reproducible, accurate quantitation of monoclonal antibodies (mAbs), associated high molecular weight aggregates (&gt;300,000 Da), and lower molecular weight fragments (e.g., &lt;100,000 Da)</li><li>Each packed column is tested with NIST-based, mAb size variant standard to help ensure batch-to-batch and column-to-column performance consistency<ul><li><a target="_blank" rel="noopener noreferrer" href="https://www.waters.com/waters/global/BioResolve-SEC-mAb-Consumables-%7C-SEC-Guards/nav.htm"><strong>Explore BioResolve mAb SEC Guards and Columns</strong></a></li></ul></li></ul><h5><strong>ACQUITY UPLC Protein BEH SEC Guards and Columns</strong></h5><ul><li>Sub-2-μm particle columns for UPLC separations (2.5 μm for 450 Å)</li><li>Pore size options include: 125 Å, 200 Å, and 450 Å</li><li>Available SEC Guard Columns to extend analytical SEC Column life</li><li>Specifically designed and QC tested with BEH125, BEH200, or BEH450 protein standards to help ensure batch-to-batch consistency</li><li>10x faster than traditional HPLC-based SEC<ul><li><a target="_blank" rel="noopener noreferrer" href="https://www.waters.com/nextgen/us/en/products/columns/acquity-uplc-columns.html"><strong>Explore ACQUITY UPLC Protein BEH SEC Guards and Columns</strong></a></li></ul></li></ul><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Waters_Acquity_UPLC_kolony_1dc5c25d3e/Waters-Acquity-UPLC-kolony.jpg" alt="Waters: Acquity UPLC columns" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Waters_Acquity_UPLC_kolony_1dc5c25d3e/thumbnail_Waters-Acquity-UPLC-kolony.jpg 245w," sizes="100vw" width="807" height="407"></p><h5><strong>XBridge Protein BEH SEC Columns</strong></h5><ul><li>2.5 μm particle columns for use on any UPLC, UHPLC, or HPLC system</li><li>Pore size options include: 125 Å, 200 Å, and 450 Å</li><li>Available SEC Guard Columns to extend analytical SEC Column life</li><li>Specifically designed and QC tested with BEH125, BEH200, or BEH450 protein standards to help ensure batch-to-batch consistency<ul><li><a target="_blank" rel="noopener noreferrer" href="https://www.waters.com/waters/global/XBridge-BEH-Columns-for-High-pH-Chromatography/nav.htm"><strong>Explore XBridge Protein BEH SEC Columns</strong></a></li></ul></li></ul><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Waters_X_Bridge_Columns_b0b55de501/Waters-XBridge-Columns.jpg" alt="Waters: XBridge Columns" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Waters_X_Bridge_Columns_b0b55de501/thumbnail_Waters-XBridge-Columns.jpg 203w," sizes="100vw" width="900" height="691"></p><h4><strong>Peptide SEC Columns</strong></h4><h5><strong>BioSuite SEC Columns</strong></h5><ul><li>Silica-based diol SEC columns</li><li>Molecular weight exclusion limits are determined primarily by the selected pore size</li><li>Available in analytical and semi-preparative column sizes<ul><li><a target="_blank" rel="noopener noreferrer" href="https://www.waters.com/waters/global/BioSuite-Columns/nav.htm"><strong>Explore BioSuite SEC Columns</strong></a></li></ul></li></ul><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Waters_Bio_Suite_Columns_bf2bb8249f.jpg" alt="Waters: BioSuite Columns" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Waters_Bio_Suite_Columns_bf2bb8249f.jpg 184w," sizes="100vw" width="307" height="260"></p><h5><strong>Protein-Pak SEC Columns</strong></h5><ul><li>Based on a 10 μm diol-bonded silica</li><li>Resolve proteins that differ in molecular weight by a factor of two</li><li>Distinguish proteins differing by as little as 15% in molecular weight<ul><li><a target="_blank" rel="noopener noreferrer" href="https://www.waters.com/waters/global/Protein-Pak-HPLC-and-UPLC-Columns/nav.htm"><strong>Explore Protein-Pak SEC Columns</strong></a></li></ul></li></ul><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Waters_Protein_Pak_Columns_be6a669326.jpg" alt="Waters: Protein-Pak Columns" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Waters_Protein_Pak_Columns_be6a669326.jpg 245w," sizes="100vw" width="500" height="315"></p><h4><strong>Choose the right SEC Column for your lab</strong></h4><p>SEC is ideal for aggregate and fragment analysis in the research, development, and manufacturing of biotherapeutic peptides, proteins, and viral vectors, but choosing the right column for your analysis is important. Use the following criteria to choose a column that provides reliable, high quality SEC analyses of various biomolecules, including insulin, monoclonal antibodies, and adeno associated viruses (AAVs).</p><h5><strong>What to consider when choosing an SEC Column:</strong></h5><ul><li>What is the molecular weight of your sample?</li><li>What is the dispersion of your LC system?</li><li>Are you separating components &lt;2 fold difference in molecular weight (MW)?</li><li>Are you separating components &gt;2 fold difference in MW?</li></ul><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Waters_Choose_the_right_Size_exclusion_chromatography_SEC_column_for_your_analysis_with_Waters_SEC_columns_selection_guide_b81f674f65.jpg" alt="Waters: Choose the right Size-exclusion chromatography (SEC) column for your analysis with Waters SEC columns selection guide." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Waters_Choose_the_right_Size_exclusion_chromatography_SEC_column_for_your_analysis_with_Waters_SEC_columns_selection_guide_b81f674f65.jpg 158w," sizes="100vw" width="1024" height="1013"></p><h4><strong>Addressing the complexity of SEC Separations</strong></h4><p>Experience the advancements in SEC-based technologies to address non-desired specific ionic and hydrophobic interactions for more reliable results with ACQUITY Premier Protein SEC and XBridge Premier Protein SEC Columns and Guards.</p><h5><strong>With MaxPeak Premier SEC Columns, you can:</strong></h5><ul><li>Reproducibly resolve injected sample components that are less than 2x different in size for challenging applications</li><li>Use a ‘platform SEC method’ to reduce method development time and cost</li><li>Ensure batch-to-batch and column-to-column performance</li></ul><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Waters_Classes_of_therapeutic_proteins_separated_on_an_ACQUITY_Premier_Protein_SEC_Column_d3f74a8ea4.jpg" alt="Waters: Classes of therapeutic proteins separated on an ACQUITY Premier Protein SEC Column." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Waters_Classes_of_therapeutic_proteins_separated_on_an_ACQUITY_Premier_Protein_SEC_Column_d3f74a8ea4.jpg 234w," sizes="100vw" width="1024" height="682"></p>

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