<h3>Two Dimensions, One Solution: Advancing Oligonucleotide Analysis with 2D-LC</h3><h4>Why Consider 2D-LC for Oligonucleotide Analysis?</h4>In oligonucleotide analytics, achieving reliable and precise results is not optional—it is fundamental. These biomolecules are inherently complex, often containing truncated sequences, structural variants, and a wide range of impurities. Such complexity frequently exceeds the separation capabilities of conventional one-dimensional liquid chromatography (1D-LC). As a result, critical components may coelute or remain unresolved, limiting analytical confidence.Two-dimensional liquid chromatography (2D-LC) offers a powerful alternative by significantly enhancing separation performance. By combining two complementary separation mechanisms, 2D-LC improves resolution, sensitivity, and overall analytical reliability. This enables a much deeper understanding of oligonucleotide samples and their compositional intricacies.<blockquote><h4>Previous parts from the Oligos Made Easy series</h4><ul><li>Part 1: <a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/article/6808">Overview</a></li><li>Part 2: <a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/article/6846">AEX</a></li><li>Part 3: <a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/article/6880">IP-RP</a></li><li>Part 4: <a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/article/6912">HILIC</a></li><li>Part 5: <a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/article/6944">SEC</a></li><li>Part 6: <a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/article/6982">Column Navigator</a></li><li>Part 7: <a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/article/7012">Large Scale AEX</a></li><li>Part 8: <a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/article/7045">Sepapure oliGO</a></li></ul></blockquote><h4>Moving Beyond the Limits of 1D-LC</h4>For many years, 1D-LC has served as a standard tool for biopharmaceutical characterization. However, its inherent limitations become apparent when dealing with highly complex mixtures. The selectivity of a single chromatographic dimension is restricted, and the lack of orthogonality in retention mechanisms often results in insufficient separation. As sample complexity increases, the resolving power of 1D-LC becomes inadequate.<img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/KNAUER_Figure_1_2_D_LC_Principle_Graphic_by_KNAUER_43e04d32a2.jpg" alt="KNAUER: Figure 1 - 2D-LC Principle (Graphic by KNAUER)." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_KNAUER_Figure_1_2_D_LC_Principle_Graphic_by_KNAUER_43e04d32a2.jpg 245w," sizes="100vw" width="1291" height="391">Two-dimensional LC addresses these limitations by coupling two chromatographic separations, which can be either similar or fundamentally different. Each dimension targets different physicochemical properties of analytes, enabling the resolution of compounds that would otherwise overlap. In essence, 2D-LC introduces an additional level of separation capacity, allowing analysts to disentangle coeluting species that remain unresolved in a single dimension.An important practical consideration is that the overall analysis time is governed by the second dimension. Because fractions from the first dimension must be processed sequentially, the second dimension must operate rapidly and efficiently. High-speed separations in the second dimension are therefore essential to maintain system performance and data quality.<h4>Architecture of a 2D-LC System</h4>At its core, a 2D-LC system relies on precise coordination between its components. The setup typically consists of two chromatographic columns, two pumping systems, and a switching valve that enables fraction transfer between dimensions. The sample is introduced through an autosampler and first separated on the primary column. Fractions of interest are then transferred into a loop via a switching valve and subsequently introduced into the second column for further separation.<img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/KNAUER_Figure_2_2_D_LC_System_Configuration_Example_Graphic_by_KNAUER_f5747adf3a.jpg" alt="KNAUER: Figure 2 - 2D LC System Configuration Example (Graphic by KNAUER)." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_KNAUER_Figure_2_2_D_LC_System_Configuration_Example_Graphic_by_KNAUER_f5747adf3a.jpg 245w," sizes="100vw" width="859" height="517">The valve plays a crucial role in this process, acting as the interface between both chromatographic dimensions. Its function is to alternately collect fractions from the first dimension and inject them into the second dimension under high-pressure conditions. Various valve configurations can be employed, including ten-port or eight-port valves with dual-loop arrangements, or synchronized six-port valves. Regardless of configuration, the underlying principle remains the same: while one loop is being filled, the other is being flushed into the second dimension.<img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/KNAUER_Figure_3_Schematic_figure_of_ten_port_left_and_eight_port_valve_right_2_position_setup_for_2_D_LC_Graphic_by_KNAUER_9209661ea8.jpg" alt="KNAUER: Figure 3 - Schematic figure of ten-port (left) and eight-port valve (right) 2-position setup for 2D-LC (Graphic by KNAUER)." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_KNAUER_Figure_3_Schematic_figure_of_ten_port_left_and_eight_port_valve_right_2_position_setup_for_2_D_LC_Graphic_by_KNAUER_9209661ea8.jpg 245w," sizes="100vw" width="1287" height="406">This alternating process requires precise timing. The second-dimension separation must be completed before the next fraction is ready for transfer. Consequently, synchronization between flow rates, loop volumes, and switching intervals is essential for maintaining chromatographic integrity.<img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/KNAUER_Figure_4_Schematic_figure_KNAUER_eight_port_2_position_valve_for_2_D_LC_Graphic_by_KNAUER_f9a5f06f5f.jpg" alt="KNAUER: Figure 4 - Schematic figure KNAUER eight-port 2 position valve for 2D-LC (Graphic by KNAUER)." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_KNAUER_Figure_4_Schematic_figure_KNAUER_eight_port_2_position_valve_for_2_D_LC_Graphic_by_KNAUER_f9a5f06f5f.jpg 245w," sizes="100vw" width="756" height="391"><h4>Applying 2D-LC to Oligonucleotide Analysis</h4>In oligonucleotide workflows, 2D-LC combines two complementary separation strategies to address sample complexity. The first dimension is typically used for bulk separation based on properties such as size, charge, or polarity. Techniques such as size-exclusion chromatography (SEC), anion-exchange chromatography (AEX), ion-pair reversed-phase chromatography (IP-RP), hydrophilic interaction chromatography (HILIC), or mixed-mode chromatography (MMC) are commonly applied. This step reduces sample complexity and can also serve as an online desalting stage, removing salts and buffer components that could interfere with downstream detection, particularly when mass spectrometry is used.Following this initial separation, selected fractions are transferred to the second dimension. This transfer can be performed either selectively or comprehensively, depending on the analytical goal. The second dimension is typically optimized for high-resolution separation, allowing detailed characterization of closely related species such as truncated oligonucleotides, sequence variants, or chemically modified impurities. When coupled with mass spectrometry, this setup enables accurate identification and sequencing.A key advantage of 2D-LC lies in the flexibility of combining different stationary phases and mobile phase conditions.&nbsp;Numerous separation combinations are possible, including:<ul><li>SEC×AEX</li><li>HILIC×IP-RP</li><li>IEC×IP-RP</li><li>IP-RP×IP-RP</li><li>AEX×HIC</li><li>MMC×SEC,</li><li>and even chiral×RP systems.&nbsp;</li></ul>This adaptability allows tailored workflows for specific analytical challenges.<h4>Heart-Cutting vs. Comprehensive 2D-LC</h4>When implementing 2D-LC, two primary transfer strategies are commonly used: heart-cutting and comprehensive transfer. Each approach offers distinct advantages depending on the analytical objective.Heart-cutting 2D-LC focuses on selected regions of interest from the first dimension. Only specific peaks or time windows are transferred to the second dimension for further analysis. This approach allows extended separation time and higher resolution in the second dimension, making it particularly suitable for targeted investigations of known critical compounds. However, because only selected fractions are analyzed, unexpected impurities outside the selected region may remain undetected.<img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/KNAUER_Figure_5_2_D_LC_Single_Heart_Cut_Principle_Graphic_by_KNAUER_b1b400784b.jpg" alt="KNAUER: Figure 5 - 2D-LC Single Heart-Cut Principle (Graphic by KNAUER)." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_KNAUER_Figure_5_2_D_LC_Single_Heart_Cut_Principle_Graphic_by_KNAUER_b1b400784b.jpg 245w," sizes="100vw" width="1276" height="319">In contrast, comprehensive 2D-LC transfers the entire eluate from the first dimension into the second dimension in a continuous manner. This approach provides a complete and detailed representation of the sample, making it ideal for research and development, impurity profiling, and regulatory applications. While more demanding in terms of speed and system performance, comprehensive 2D-LC ensures full sample coverage.<img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/KNAUER_Figure_6_Full_Comprehensive_2_D_LC_Principle_Graphic_by_KNAUER_e13da63ef1.jpg" alt="KNAUER: Figure 6 - Full Comprehensive 2D-LC Principle (Graphic by KNAUER)." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_KNAUER_Figure_6_Full_Comprehensive_2_D_LC_Principle_Graphic_by_KNAUER_e13da63ef1.jpg 245w," sizes="100vw" width="1327" height="292">In practice, hybrid approaches may also be employed, combining targeted heart-cutting with broader comprehensive analysis to balance resolution and coverage.<h4>Practical Considerations for Method Development</h4>Successful implementation of 2D-LC for oligonucleotide analysis requires careful method design.&nbsp;<h5>1. Column selection&nbsp;</h5>It is a critical starting point, as the combination of stationary phases must provide sufficient orthogonality while maintaining compatibility between mobile phases. Typically, the first dimension is optimized for general separation of oligonucleotide size or class, while the second dimension focuses on sequence-specific resolution and compatibility with mass spectrometry.<h5>2. Mobile phase selection&nbsp;</h5>Differences in pH and organic modifiers can significantly influence separation behavior, but incompatibility between dimensions can compromise performance. Therefore, mobile phases must be designed as a coordinated system. The use of volatile buffers, such as ammonium acetate, is often preferred for MS-based detection.<h5>3. Instrumentation&nbsp;</h5>The switching valve must operate reliably under high pressure, with precise timing to ensure accurate fraction transfer. Loop volumes must be carefully selected to avoid overfilling, as excessive loading can lead to sample loss and distortion due to flow profile effects. As a general guideline, loops should not be filled beyond approximately 70% of their capacity.<h5>4. Method development&nbsp;</h5>Typically begins with optimization of the second dimension, as it dictates overall system timing. Fast separations using short columns and steep gradients are often required to maintain sufficient sampling frequency. Once the second dimension is established, the first dimension can be adjusted to achieve optimal resolution and compatibility.<h4>Final Remarks</h4>Two-dimensional liquid chromatography represents a powerful advancement in oligonucleotide analysis, enabling the resolution of complex mixtures that exceed the capabilities of conventional 1D approaches. By combining orthogonal separation mechanisms, 2D-LC reveals structural details and impurity profiles that would otherwise remain hidden.However, achieving optimal performance requires careful balancing of multiple factors, including column chemistry, mobile phase compatibility, system timing, and data acquisition. When properly implemented, 2D-LC provides a robust and highly informative platform for modern oligonucleotide characterization.<blockquote><ul><li>If you're planning to set up or optimize your oligonucleotide workflow, feel free to contact us at <a href="sales@knauer.net">sales@knauer.net</a>. Stay tuned for more exciting insights into the Oligonucleotide world in our “Oligos Made Easy” series.</li><li>For more in-depth discussion or questions, reach out to the author at <a href="kindler@knauer.net">kindler@knauer.net</a></li></ul></blockquote>

Oligos Made Easy - Part 9: 2D-LC

News from LabRulezLCMS Library - Week 14, 2026

<h3>We're excited to announce that <a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/products/999">Clarity Chromatography Software</a> version 10.1.2 is now available!</h3>This minor update expands Clarity’s portfolio with new control modules.<h4>Newly added Instrument control modules</h4><ul><li>Ecom auxiliary modules</li><li>HTA ICF control modules</li><li>Spark pump</li></ul><h4>Updated Instrument control modules</h4><ul><li><a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/companies/168">Ecom</a> HPLC</li><li><a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/companies/328">Knauer</a> HPLC</li><li>Spark ICF control modules</li><li>YoungIn Chromass HPLC</li></ul><blockquote>For a complete list of module changes, see the <a target="_blank" rel="noopener noreferrer" href="https://www.dataapex.com/version_history?product=1">Changelog</a>. For more info about exciting new features directly in Clarity, explore what was introduced in the <a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/article/5622">Clarity 10.1 release highlights</a>.Clarity can control 1000+ instruments; the <a target="_blank" rel="noopener noreferrer" href="https://www.dataapex.com/controls">list can be previewed here</a>.</blockquote><h4>Supported Instruments in Clarity (DataApex) — Quick Guide</h4>Clarity Chromatography Software supports a wide range of chromatographs and peripherals via digital control (DD) and analog acquisition (A/D) using the Colibrick module.<h5>Gas Chromatographs (GC)</h5>Digital integration over LAN/RS232 (many via ICF):<ul><li>Agilent: 5890, 6890, 7890, 8860, 8890, Intuvo 9000</li><li>Shimadzu: GC-2010/2014, Nexis GC-2030</li><li>Thermo Fisher: TRACE 1300/1310</li><li>PerkinElmer: Clarus 400–690</li><li>Also supported: Dani, Ellutia, Young In Chromass, Apix, Fuli, GOW-MAC (selected models)</li></ul><blockquote>Note: Most Agilent 6890–8890 and Intuvo use LAN – ICF.</blockquote><h5>HPLC Systems</h5>Full system/module control:<ul><li>Agilent 1100–1290 Infinity II (ICF)</li><li>Shimadzu LC-10/20 and Nexera LC-40</li><li>PerkinElmer Flexar / NexSAR</li><li>Knauer Azura / BlueShadow / Smartline</li><li>Hitachi Chromaster / Primaide</li><li>Young In Chromass ChroZen series</li><li>Sykam S500–S600, Watrex Streamline, Hanon LC7000–7300</li></ul><h5>HPLC Modules (Pumps, Detectors, Ovens, Fraction Collectors)</h5><ul><li>Pumps: Agilent 1100–1290 families, Knauer, Shimadzu, PerkinElmer, Sykam, SSI, Ecom</li><li>Detectors: VWD/DAD(PDA)/RID/FLD/ELSD across Agilent, Shimadzu, Knauer, PerkinElmer, Young In Chromass, Ecom, etc.</li><li>Column Ovens: Agilent G1316x / G7116x, Shimadzu CTO-20/30/40, Knauer CT2.1, PerkinElmer Flexar</li><li>Fraction Collectors: Knauer FC 6.1, Teledyne FOXY series, Ecom ECF 2096, Waters Fraction Collector II</li></ul><h5>Autosamplers</h5><ul><li>Agilent GC ALS (7673/7683/7693/8697), HPLC ALS modules (G1313A–G7167C)</li><li>Shimadzu AOC-20 / AOC-5000+, SIL-10 / SIL-20 / SIL-40 series</li><li>CTC Analytics PAL, HTA autosamplers, Spark Alias/UHPLC, Knauer AS 6.1L</li><li>PerkinElmer Flexar / NexSAR AS</li></ul><h5>Other Devices</h5><ul><li>GC×GC modulators (e.g., Charles University GC×GC Modulator)</li><li>Valves &amp; switch modules (Agilent, Knauer, Shimadzu, VICI-Valco)</li><li>Degassers, flow monitors, managers, interfaces</li><li>LC-MS / GC-MS: Advion Expression CMS, Microsaic 4500 MiD, Markes BenchTOF (and others)</li><li>Balances (EA extension): Mettler Toledo, Sartorius</li></ul><h5>Analog Acquisition (Colibrick)</h5>A/D support for legacy or analog-output devices:<ul><li>GC: Agilent 5880/4890A, Varian 3xxx/3800/3900, PerkinElmer Clarus series, Thermo TRACE, Shimadzu GC-14/9A, Scion 43x/45x/83xx/85xx, etc.</li><li>HPLC Detectors: Waters 2487/2489, ESA Corona, Hitachi L-series, JASCO FP/UV, Ecom LCD series, many others</li><li>Elemental &amp; Amino Acid Analyzers: Carlo Erba/Costech ECS, Biochrom 20/30+</li></ul>If you are interested in a DEMO VERSION, contact us!

DataApex Clarity Chromatography software 10.1.2 just released!

Article

Product

Application

Academy

Software

Since 1991 DataApex develops and manufactures chromatography data stations. DataApex is constantly striving to provide users with efficient and up-to-date tools for chromatography data processing.

DataApex

Everything from the world of analytical chemistry in one place. We connect people in solving their problems. At Labrulez you will find all the necessary information easily, quickly and clearly. Stop searching and start finding.

LabRulez

Based in Berlin, KNAUER is a medium-sized, owner-managed company that has been serving the sciences since 1962. We develop and manufacture scientific instruments of superior quality for liquid chromatography, including: Analytical HPLC/UHPLC, Preparative HPLC, Fast protein liquid chromatography (FPLC), Multi-column chromatography/Simulated moving bed (SMB) chromatography. High pressure dosing, in-line detection systems, and osmometry are additional business areas.

KNAUER

Agilent Technologies

Technology Networks

Shimadzu Corporation

SCIEX

Waters Corporation

In this webinar the objective is to explore the unique mixed mode acquisition capability of the Agilent Ultivo Triple Quadrupole mass spectrometer, which allows for the sequential use of different acquisition modes in a single injection.
Examples of field applications where mixed mode analysis would be fundamentally valuable will also be discussed
<blockquote>
Presenter: Dr. Julie Cichelli (Agilent LC/MS applications Scientist, Agilent Technologies, Inc.)
</blockquote>
<blockquote>
Julie Cichelli obtained her Bachelor’s Degree from Villanova University in 2003 with a major in chemistry and a minor in mathematics. She went on to pursue graduate school at the University of Utah, studying under Dr. Zharov, completing her Ph.D. in Organic Chemistry in 2008. Following graduate school in 2009 she accepted a postdoctoral fellowship at the Huntsman Cancer Institute in Salt Lake City researching colon cancer under Dr. Stafforini and Dr. Topham. Subsequently, she joined the Agilent LC/MS applications team in 2010, with a focus in clinical and forensic applications.
</blockquote>

Agilent Technoligies: LC/MS QQQ acquisition / MassHunter Quantitative Analysis

LC/MS QQQ acquisition / MassHunter Quantitative Analysis

Unique mixed mode acquisition capability of the Agilent Ultivo LC/QQQ, which allows for the sequential use of different acquisition modes in a single injection.

Join us for our extended webinar to hear about the most recent and innovative developments in the world of mass spectrometry and how they are helping to drive science forward.
Mass spectrometry has become an indispensable tool for many industries, from pharmaceutical and drug development to food and environmental analysis. Constant development and improvements are helping to make the technologies more accessible, easier to use, more sensitive and faster whilst reducing their footprint both physically and environmentally.
This Technology Networks event features exclusive talks focused on the challenges faced by analysts and how new solutions are helping to solve questions and address pain points.
<h3>Attend this webinar to:</h3>
<ul>
<li>Hear about ways in which mass spectrometry is being utilized to solve novel questions</li>
<li>Explore some of the challenges and limitations of existing technology and how they are being overcome</li>
<li>Look at the future direction of mass spectrometry R&amp;D</li>
</ul>
<blockquote>
Expert Speakers: Anyin Li (Assistant Professor, University of New Hampshire)
</blockquote>
<blockquote>
Expert Speakers: Patrick Batoon ( Product Manager of Triple Quadrupole LC/MS, Agilent Technologies Inc.)
</blockquote>
<blockquote>
Expert Speakers: Ihor Batruch (Technical Product Manager, Accurate Mass, SCIEX)
</blockquote>
<blockquote>
Expert Speakers: Marcel Niehaus (MALDI-TOF Instrumentation R&amp;D, Bruker)
</blockquote>
<blockquote>
Expert Speakers: Ron Heeren (Distinguished Professor, Maastricht Multimodal Molecular Imaging Institute)
</blockquote>

Technology Networks: Innovations In Mass Spectrometry

Innovations In Mass Spectrometry

Join us for our extended webinar to hear about the most recent and innovative developments in the world of mass spectrometry.

This two-day event will be a unique opportunity to network with your peers and learn about their successes.
WEOF 2022 will take a hybrid format, with options to attend the event in person either in Wilmslow, UK or in Guyancourt (Paris area), France. Virtual attendees will have access to oral lecture content but will be unable to attend our hands on, interactive sessions or the conference dinner 
<h3>WEOF 2022 Agenda</h3>
Our agenda will be a mix of talks from the scientific community, Waters’ presentations to better address your analytical needs and live demos on LC/MS and software.
<h5>Topics will include:</h5>
<ul>
<li>Existing and new analytical methods for synthetic oligonucleotides</li>
<li>Existing and new analytical methods for gene therapy therapeutics</li>
<li>Charge detection Mass Spectrometry</li>
<li>Latest hardware and software developments for all nucleic acid based therapeutics</li>
</ul>
<h3>WEOF 2022 Agenda</h3>
<h5>9.30 - 10.30 Introduction and Agenda Paris</h5>
<h5>9.40 - 10.40 Waters Solutions for Gene Therapy Analytics</h5>
<ul>
<li>Evelien Dejaegere, Waters Corporation</li>
<li>Paris</li>
</ul>
<h5>10.35 - 11.35 Break*</h5>
<h5>10.55 - 11.55 LC Methods for AAV Analysis</h5>
<ul>
<li>Patrick Hoering, Pharmaron</li>
<li>Wilmslow</li>
</ul>
<h5>11.20 - 12.20 An Overview of Charge Detection Mass Spectometry [CDMS]</h5>
<ul>
<li>Steve Preece, Waters Corporation</li>
<li>Wilmslow</li>
</ul>
<h5>11.45 - 12.45 Lunch*</h5>
<h5>12.45 - 13.45 Developments in Analytics for mRNA</h5>
<ul>
<li>Nick Pittman, Waters Corporation</li>
<li>Wilmslow</li>
</ul>
<h5>13.05 - 14.05 Analysis of mRNA on the BioAccord System</h5>
<ul>
<li>Filip Bergqvist, Cytiva</li>
<li>Paris</li>
</ul>
<h5>13.30 - 14.30 Aggregation, Titre and Full/empty Analysis of AAVs by Liquid Chromatography</h5>
<ul>
<li>Waters Corporation*</li>
<li>Local Programme</li>
</ul>
<h5>14.10 - 15.10 Break*</h5>
<h5>14.30 - 15.30 Identity and Modification of AAV Capsid Proteins by LC-MS. Peptide Mapping</h5>
<ul>
<li>Waters Corporation*</li>
<li>Local Programme</li>
</ul>
<h5>15.10 - 16.10 mRNA and Lipid Nanoparticle Workshop</h5>
<ul>
<li>Waters Corporation*</li>
<li>Local Programme</li>
</ul>
<h5>15.50 - 16.50 Close</h5>

Waters Corporation: Waters European Oligonucleotide Forum

Waters European Oligonucleotide Forum 2 - Day 2

We are delighted to continue our annual series dedicated to the oligonucleotide analytical community in Europe.

LC/MS is now widely used for many clinical and forensic toxicology applications due to the inherent specificity and sensitivity of the technology. Sample preparation and pre-treatment requirements of LC/MS can still be a bottle neck in high volume testing laboratories resulting in time consuming and labor intensive manual steps.
This presentation will describe how automation of LC/MS methods can improve productivity and analytical results.
Explore the journey of the sample through an automated LC/MS workflow from sample receipt to the reporting of results back into a LIMS. The advantages of automation are discussed, along with automation options for the toxicology LC/MS workflow based on different laboratory needs. Sample tracking is a key part of any workflow and has been considered in all automated options shown, including other important requirements to ease the extraction and analysis process for the analyst.
<h5>Learning Objectives - What you will learn:</h5>
<ul>
<li>Why Automation is important for LC/MS workflows in toxicology applications
</li>
<li>What steps can be automated in LC/MS workflows
</li>
<li>How automation can improve analytical performance and productivity
</li>
</ul>
<blockquote>
Presenter: Robert Wardle (Senior Scientist, Clinical Applications, Waters Corporation, UK)
</blockquote>
<blockquote>
Rob Wardle is a currently a Senior Scientist employed at Waters Corporation in Wilmslow, UK, performing research and development of applications and products for Clinical and toxicology applications. Rob has wide experience of method development in clinical and toxicology applications and in recent years has played a key role on developing offline and online automation methods and protocols. Rob joined Waters in 2010 as a Clinical Validation Scientist, playing a key role in verification activities for LC/MS reagent kits. Rob moved into his current role as a Senior Scientist in 2013, where he developed methods for a range of applications including vitamin D metabolites, vitamin B1 and B6 metabolites and a large panel of steroid hormones. Rob have presented work at conferences worldwide, gaining my first publication in Clinica Chimica Acta as well as having a patent application pending.
</blockquote>

Waters Corporation: Automating Toxicology LC/MS workflows – Improving productivity and analytical performance through automation

Automating Toxicology LC/MS workflows – Improving productivity and analytical performance through automation

This presentation will describe how automation of LC/MS methods can improve productivity and analytical results.

Automating Toxicology LC/MS workflows

<h3>Top 3 takeaways:</h3>
<ul>
<li>The ability to detect and quantify fluticasone propionate and salmeterol xinafoate down to 0.050 pg/mL when using the SCIEX 7500 system, in a human plasma matrix
</li>
<li>Excellent levels of precision and accuracy at every concentration level analysed
</li>
<li>Ultra-low level quantification within an 8 minute run time, delivering high quality data along with excellent throughput
</li>
</ul>
<blockquote>
Presenter: Jack Steed (Technical Specialist, SCIEX)
</blockquote>

SCIEX: How to determine very low pg/ml LLoQ in human plasma of anti-asthmatic drugs

How to determine very low pg/ml LLoQ in human plasma of anti-asthmatic drugs

The ability to detect and quantify fluticasone propionate and salmeterol xinafoate down to 0.050 pg/mL in a human plasma matrix

Very low pg/ml LLoQ in human plasma of anti-asthmatic drugs

Shimadzu Total Lab for Biopharma provides innovative and versatile analytical solutions for the biopharmaceutical market with a comprehensive range of instruments including LC/MS and Spectroscopy.
In this seminar we would like to introduce analytical solutions developed at Shimadzu that can help in establishing workflow for mAb discovery, clinical evaluation, biosimilar characterization, process development and QA/QC for batch release testing.
<h5>Learning Objectives:</h5>
<ul>
<li>Structure and funciton of monoclonal antibody
</li>
<li>Shimadzu application for mAb characterization 
</li>
<li>The range of instruments Shimadzu can offer for these workflow
</li>
</ul>
<blockquote>
Presenter: Dr. Wang Tianhua (Product Specialist, Shimadzu Asia Pacific)
</blockquote>
<blockquote>
Tianhua did his PhD in University of Pennsylvania (Chemical and Biomolecular Engineering). He then spent 5 years in the analytics group at Bioprocessing Technology Institute. His research interest includes drug discovery for infectious disease (SARS-CoV and Ebola), biopharmaceutical production system optimization and mAb Characterization with particular focus in glycan profiling and glycomics study. Tianhua joined Shimadzu in 2017 as a product specialist for life science products.
</blockquote>
<blockquote>
Presenter: Nicole Zhang (Senior Product Specialist, Shimadzu Asia Pacific)
</blockquote>
<blockquote>
Nicole studies in UK in Microbiology and Immunology, prior joining Shimadzu, she worked in different companies in analytical instrument industry for more than 10 years, from Chemist to marketing and in charge of different technologies from LC, MS to Spectro products. She joined Shimadzu at the year of 2018 as spectroscopy product specialist and in charge of food quality and safety market.
</blockquote>

Shimadzu: Integrating powers of MS and Spectroscopy - Solutions for mAB characterization

Integrating powers of MS and Spectroscopy - Solutions for mAB characterization

Analytical solutions developed at Shimadzu that can help in establishing workflow for mAb discovery.

Integrating powers of MS and Spectroscopy

Presentation Topics
<ul>
<li>Origins of the Digital Ion Trap
</li>
<li>Ion Trap v. Digital Ion Trap
</li>
<li>Shimadzu&#39;s Journey to the MALDI Digital Ion Trap
</li>
<li>Basic Proteomics
</li>
<li>Glycomics
</li>
<li>Antibody Drug Conjugates
</li>
<li>Summary and Acknowledgements
</li>
</ul>

Shimadzu: Proteomic Analysis Using a Miniature MALDI Digital Ion Trap Mass Spectrometer

Proteomic Analysis Using a Miniature MALDI Digital Ion Trap Mass Spectrometer

Proteomic Analysis Using a Miniature MALDI Digital Ion Trap

The hunt for novel drug candidate is a tedious and time-consuming process. A good workflow efficiency, from start of chemistry to purification to structural elucidation should streamline the discovery process and increase the success rate of developing novel candidate compound.
In this Mass Discussion, Dr. Ramesh Appadurai from Syngene International and Pottayil G Sasikumar from Aurigene Discovery Technologies will share their experience in running successful R&amp;D pipeline with the latest technology advancement. Let us also take a brief glimpse of Shimadzu&#39;s innovative drug discovery solutions which are designed to provide comprehensive and holistic workflow solution, ensuring faster result generation and maximizing ROI
<h5>Learning Objectives:</h5>
<ul>
<li>Analytical approaches for successful and innovative drug discovery pipeline
</li>
<li>Shimadzu&#39;s unique Drug Discovery Workflow
</li>
<li>Peptide-based drug candidates for cancer immunotherapy
</li>
</ul>
<blockquote>
Presenter: Dr Ramesh Appadurai (Research Director – Discovery Analytical Chemistry, Syngene International)
</blockquote>
<blockquote>
Dr Ramesh Appadurai Research Director and Head of Discovery Analytical Chemistry’ for Syngene International, a Contract Research Organization [CRO] company. He has over 16 years plus experience in this field Discovery Analytical Chemistry. Being in Discovery Analytical Lab, his key expertise is in developing efficient purification workflows.
</blockquote>
<blockquote>
Presenter: Dr. Pottayil G Sasikumar (Independent consultant and Advisor - Peptide Chemistry, Aurigene Discovery Technologies Limited, Bangalore)
</blockquote>
<blockquote>
Dr. Pottayil G Sasikumar has more than 15 years of experience in peptide therapeutics and peptide/amino acid inspired small molecule drug discovery. He joined Aurigene Discovery Technologies Limited in 2006, and established the peptide department. As Research Director, he headed various aspects of peptide therapeutics including the lead optimization and CMC activities. His research mainly focuses on innovative chemical biology research fundamentally linked to outstanding immunological questions, to create new solutions for immune-related diseases. He is the co-inventor of an anti-cancer drug CA-170 (First orally bioavailable checkpoint inhibitor- Immuno-oncology agent) currently in Phase 2b/3 clinical trials and several other drugs which are in various phases of clinical development. 
</blockquote>
<blockquote>
Presenter: David Chan (Assistant Product Manager, Shimadzu (Asia Pacific) Pte. Ltd)
</blockquote>
<blockquote>
As LC Product Manager, David Chan is based in Shimadzu Asia Pacific, managing and handling the LC product line in the region. Before joining Shimadzu Asia Pacific, David was a Drug Discovery Chemist with more than 10 years of experience in small molecule design and synthesis. He understands the day-to-day difficulties faced by the synthetic chemist well through his extensive working experience, and knows how to apply the correct technology advancement to overcome these issues, in order to streamline the tedious discovery process.
</blockquote>

Shimadzu: Toolkit for Treasure Hunt - Drug Discovery Made Easy

Toolkit for Treasure Hunt - Drug Discovery Made Easy

Running successful R&D pipeline with the latest technology advancement.

The COVID-19 pandemic has brought unprecedented challenges for the communities and economies worldwide, causing a profound effect on the lives of billions of people.
While government and communities are trying to contain the spread, researchers are aggressively focusing their efforts towards finding a treatment and/or vaccine. Science is more important now than ever before.
This webinar will feature the current analytical technologies and solutions that Shimadzu is committed to provide – such as analytical techniques for drug testing and vaccine research, production and testing of sanitizers and personal protective equipment and analysis of medical sewage waste.
<h5>Learning Objectives:</h5>
<ul>
<li>Comprehensive workflow for Pharma, PPE manufacturing and Environmental monitoring
</li>
<li>Explore solutions accelerating research
</li>
<li>Informatics workflows enabling remote operations and monitoring
</li>
</ul>
<blockquote>
Presenter: Sandhya (Sandy) Nargund (Deputy General Manager, Shimadzu (Asia Pacific) Pte. Ltd.)
</blockquote>
<blockquote>
Sandy has over 22 years’ experience in Analytical field with various roles starting from Pharma QC to Mass Spectrometry expert in supporting advance MS technologies. Sandy has wide experience on Mass Spectrometry while working as ‘Application Specialist’ and has conducted many users training and workflow trainings for small molecules and proteomics using LC-MS/MS, GC-MS/MS and High-resolution MS. She is currently working as Deputy General Manager in Shimadzu Asia Pacific, Singapore and is responsible for managing and promoting Shimadzu&#39;s analytical products range including Spectroscopy, Chromatography and Mass Spectrometry in region and to provide technical support on various applications in food safety, Pharma, Clinical and other markets.
</blockquote>
<blockquote>
Presenter: Jackie (Product Specialist (GC &amp; GCMS), Shimadzu (Asia Pacific) Pte. Ltd.)
</blockquote>
<blockquote>
After graduating from the National University of Singapore, Jackie has garnered over 6 years of research, training and facilitation experience. Jackie meets scientists from various industries to share technical knowledge and collect feedback, understanding their problems and helping them find well-suited solutions in GC-related analyses.
</blockquote>

Shimadzu: Analytical Technologies for COVID-19 Challenges: Drug Repurposing | Vaccine Research | PPE & Material Testing | Environmental Monitoring

Analytical Technologies for COVID-19 Challenges: Drug Repurposing | Vaccine Research | PPE & Material Testing | Environmental Monitoring

The current analytical technologies and solutions that Shimadzu is committed to provide.

Analytical Technologies for COVID-19 Challenges

There is a shifting trend in the analytical platform from immunoassay to mass spectrometry in clinical research and diagnostic lab in the past decade.
This growing trend of mass spectrometry application in clinical lab is due to the superior selectivity and sensitivity of mass spectrometers. More importantly, mass spectrometry can analyse multiple compounds in a single analysis using much lesser sample amount compared to immunoassay. The application of mass spectrometry in various clinical analysis using Shimadzu’s Ultra Fast Mass Spectrometry (UFMS) Technology will be discussed in this webinar. Lastly, Shimadzu latest innovative technology in clinical automation and rapid screening will also be highlighted.
Learning Objectives:
<ul>
<li>Introduction, background and advantages of mass spectrometry in clinical labs
</li>
<li>Understand how you can use LC-MS/MS in various clinical analyses
</li>
<li>Understand Shimadzu’s latest innovative technology in clinical automation and rapid screening.
</li>
</ul>
<blockquote>
Presenter: Daryl Hee (Ph.D.) (Senior Product Specialist (LCMS), Shimadzu (Asia Pacific) Pte Ltd)
</blockquote>
<blockquote>
Daryl Hee is currently the Senior Product Specialist for LCMS at Shimadzu (Asia Pacific) Pte Ltd. He completed his PhD study at the National University of Singapore (NUS) in 2010. Following that, he continued his postdoctoral research at the Department of Medicine NUS in clinical trial pharmacokinetics studies and conducted lectures on drug and metabolite analysis using LCMS. He is currently also holding the Adjunct Senior Research Fellow position with NUS medicine Department.
</blockquote>

Shimadzu: Brace Yourselves: The Shift to Mass Spectrometry for Clinical Analysis

Brace Yourselves: The Shift to Mass Spectrometry for Clinical Analysis

The application of mass spectrometry in various clinical analysis using Shimadzu’s Ultra Fast Mass Spectrometry (UFMS) Technology.

LCMS webinars focusing on LC/MS - page 105