<div><p><strong>Purifying the target protein is crucial for investigating its specific properties and interactions with biomolecules like DNA, RNA, and other proteins. A range of purification strategies is available, and the optimal approach depends on factors such as the required scale, throughput, and downstream application. In many cases, the process must be fine-tuned through empirical optimization to achieve the desired purity and yield.</strong></p></div><div><div><div><div><h3><strong>What is Protein Purification?</strong></h3><p>Protein purification is a critical process in biotechnology and life sciences, aimed at isolating a specific protein from complex biological mixtures such as cell lysates or tissue extracts. The goal is to obtain a highly purified protein sample, free from contaminants like other proteins, nucleic acids, and lipids, to enable accurate analysis of its structure, function, and interactions.</p><p>This process is especially vital in biotechnological areas inside immunology and microbiology. In <strong>immunology</strong>, it underpins the production of vaccines, therapeutic antibodies, and diagnostic assays by enabling the isolation of key immune proteins and antibodies. It also supports the investigation of cell signaling and immune response mechanisms. In <strong>microbiology</strong>, purified proteins are essential for studying pathogens, identifying disease-related antigens, and driving drug discovery efforts. Ultimately, protein purification is fundamental to the development of targeted vaccines, innovative diagnostics, and effective therapeutic strategies.</p></div></div></div></div><div><div><div><div><h3><strong>Types of Protein Purification Methods</strong></h3></div></div></div></div><div><p>There are several types of protein purification methods, each chosen based on the protein’s characteristics such as size, charge, solubility, and binding affinity. Below are some commonly used methods:</p></div><div><div><div><div><h4><strong>Non-Chromatographic Approaches</strong></h4></div></div><div><p>Non-chromatographic approaches use simpler physicochemical principles—such as solubility, molecular size, or diffusional properties—to separate and purify proteins. These techniques are often employed as initial purification or concentration steps before further refinement.</p><h5><strong>Precipitation Techniques</strong></h5><p>Protein precipitation is a widely used approach in purification workflows, typically relying on one of three main methods:</p><ul><li><strong>Organic solvent precipitation</strong> involves adding solvents like ethanol or acetone to lower the solution's dielectric constant. This promotes protein–protein interactions, reducing solubility and leading to aggregation and precipitation.</li><li><strong>Isoelectric point precipitation </strong>exploits the pH at which a protein carries no net charge. At this point, solubility is minimized, causing the protein to precipitate from solution.</li><li><strong>Nonionic polymer precipitation</strong> uses polymers such as dextran, polyethylene glycol (PEG), or NPEP to alter the solution’s physical properties. These agents create crowding effects that promote protein aggregation without directly binding to the proteins.</li></ul><h5><strong>Dialysis</strong></h5><p>Dialysis involves placing a protein solution inside a semi-permeable membrane that allows water and ions to pass while the protein remains inside, facilitating buffer exchange.This method is commonly used during protein expression and purification workflows for buffer exchange, desalting, and removal of unwanted small molecules such as imidazole, urea, or ammonium sulfate. The process helps maintain protein stability and activity by gradually equilibrating the sample with the desired buffer conditions.</p><h5><strong>Immunocapture (Magnetic Bead-Based Purification</strong></h5><p>Immunocapture uses antibodies immobilized on solid supports like magnetic beads to selectively bind and isolate a target protein. This method is highly specific and particularly useful for low-abundance targets or small-scale prep, such as:</p><ul><li>Pulling down proteins from complex mixtures</li><li>Capturing tagged recombinant proteins (e.g., FLAG, HA, or His tags)</li><li>Enrichment before western blotting, MS, or ELISA</li></ul><p>Magnetic beads offer speed, ease of use, and minimal sample loss, making them ideal for lab-scale workflows.</p><p><a target="_blank" rel="noopener noreferrer" href="https://www.phenomenex.com/products/biozenspe-sample-preparation-products/biozen-magbeads">Biozen MagBeads</a> provide a fast and efficient method for purifying, isolating, and cleaning up monoclonal antibodies (mAbs), proteins, and peptides. These paramagnetic beads feature a streptavidin-coated surface, enabling high-affinity binding in immuno-based workflows. Compared to traditional sample preparation techniques, magnetic bead-based immunocapture offers greater speed and consistency by delivering high binding capacity with uniform particle size, ensuring accurate and reproducible results in significantly less time.</p><h5><strong>Centrifugation</strong></h5><p>Centrifugation of crude protein mixtures is an essential step in the investigation of protein purification and aggregation. Outlined below are the various types of centrifugations employed in protein purification.</p><p><strong>Preparative Ultracentrifugation</strong><br>Ultracentrifugation is an effective method of purifying proteins from aggregates based on their size, shape and density. Centrifugation, widely recognized as a robust and versatile method, is commonly used to separate complex mixtures of cellular components. Ultracentrifuges separate small particles by spinning at high speeds, utilizing centrifugal force based on size and density.</p><p><strong>Differential Centrifugation</strong><br>Differential centrifugation operates by separating components based on differences in size and density, using varying speeds of centrifugation to achieve this. Its resolving power ranges from low to medium. A key advantage of this technique is its convenience for processing multiple samples simultaneously, making it effective for purifying crude samples. Additionally, it preserves cell viability and maintains the natural distribution of hematopoietic cell populations.</p><p><strong>Rate-zonal Gradient Centrifugation</strong><br>This technique separates components based on their sedimentation rate within a medium that contains a density gradient. It offers medium resolving power and is particularly effective for separating multiple components in complex mixtures with high precision. It is also considered a cost-effective and widely used technique in molecular biology and biochemistry.</p><p><strong>Isopycnic Centrifugation (Equilibrium Centrifugation)</strong><br>Isopycnic centrifugation is an advanced protein purification method that relies on the principle of separating particles according to their intrinsic buoyant density, irrespective of their size or morphology. During the process, proteins migrate through a density gradient until they equilibrate at a position where their density matches that of the surrounding medium, resulting in precise separation without further sedimentation.</p></div></div></div><div><div><div><div><h4><strong>Chromatographic Approaches</strong></h4><p>Chromatography is a common method of protein purification and is based on the separation of molecules in a mixture as they move through a stationary phase and a mobile phase. Outlined below are the various types of chromatography employed in protein purification.</p><h5><strong>Ion Exchange Chromatography</strong></h5><p><a target="_blank" rel="noopener noreferrer" href="https://www.phenomenex.com/techniques/hplc-ion-exchange">Ion exchange chromatography</a> relies on the interaction between a charged stationary phase and oppositely charged analytes, with cation exchange chromatography attracting positively charged molecules to a negatively charged stationary phase. Successful application of this technique requires careful selection of the appropriate ion exchange column, buffer pH, and salt concentration. This approach is often used in the biopharmaceutical industries as mAb polishing steps, viral clearance and charge variant analyses as part of the product quality control.</p><p><a target="_blank" rel="noopener noreferrer" href="https://www.phenomenex.com/">Phenomenex</a> offers a range of protein purification columns. Their ion exchange chromatography solutions include:&nbsp;</p><ul><li><a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/products/1526"><strong>Luna SCX </strong></a>for strong selectivity of positively charged compounds, such as peptides</li><li><a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/products/1540"><strong>Biozen WCX</strong></a> for efficient separation of proteins such as monoclonal antibodies charged variants</li><li><a target="_blank" rel="noopener noreferrer" href="https://www.phenomenex.com/part/00g-3151-e0">PhenoSphere SAX</a>, a strong anion-exchanger with fully porous 80Å particles, particularly useful for small molecules such as nucleotides</li></ul><h5><strong>Size Exclusion Chromatography</strong></h5><p><a target="_blank" rel="noopener noreferrer" href="https://www.phenomenex.com/techniques/hplc-sec">Size exclusion chromatography (SEC)</a> separates molecules based on their size, with larger analytes eluting first due to their exclusion from the pores in the column packing material, while smaller molecules are able to enter the pores, facing a longer travelling path, and hence, eluting later. This method can be categorized into aqueous SEC (gel filtration chromatography, GFC) for analyzing biomolecules such as proteins and antibodies. This type of chromatography is widely used as an analytical tool for quality control in the biopharmaceutical industry and can also be an approach of choice for polishing steps.</p><p>Phenomenex offers a range of <strong>Biozen columns for SEC</strong> separations. In the case of mAbs, <a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/products/1534"><strong>Biozen dSEC-2</strong></a> and<a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/products/1537"><strong> Biozen dSEC-7 </strong></a>columns are the top choices for monoclonal antibody (mAb) analysis, delivering high-resolution separation of monomers, aggregates, and fragments formed during mAb manufacturing, processing, or storage. The dSEC-7 column, featuring a larger pore size and optimized particle design, extends the dynamic range to accommodate larger biomolecules and complex biotherapeutics.</p><p>For more general applications, <a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/products/1533"><strong>Biozen SEC-2</strong></a><strong> </strong>and <a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/products/1532"><strong>Biozen SEC-3</strong></a> phases cover different MW ranges for high-efficiency separation of biomolecules (e.g., proteins and antibodies). Additionally, the BioSep and Yarra lines become top choices when looking for preparative scale columns.</p><h5><strong>Reversed-Phase Chromatography</strong></h5><p><a target="_blank" rel="noopener noreferrer" href="https://www.phenomenex.com/techniques/hplc-reversed-phase">Reversed-phase chromatography</a>, the most common mode in high-performance liquid chromatography/ ultra-high-performance liquid chromatography (HPLC/UHPLC), separates compounds based on hydrophobic and van der Waals interactions using alkyl (C18, C8, C4) stationary phases or other hydrophobic polymers. For large biomolecules such as proteins and peptides, <a target="_blank" rel="noopener noreferrer" href="https://www.phenomenex.com/resources/blog/wider-pore-biozen-column">wide-pore</a> (typically 300 Å) C4, C8 and C18 columns are ideal, as the larger pore size allows better accessibility of the protein molecules to the stationary phase surface, improving mass transfer and peak shape during separation.</p><p>The Biozen line from Phenomenex offers a wide range of HPLC/UHPLC columns for the analysis of biomolecules.These include phases optimized for high-resolution analysis of intact proteins (Native-RP, Widepore C4, Intact XB-C8) and peptides (Peptide PS-C18, Peptide XB-C18 and Peptide Polar-C18). Kinetex could also be an option when looking for more selectivities for the analysis of peptides and small proteins. Additionally, when aiming to preparative scale processes, several of these media are also available in Phenomenex Axia formats to easily up-scale your separations.</p><h5><strong>Hydroxyapatite Chromatography</strong></h5><p>Hydroxyapatite chromatography (HAC) is a sophisticated mixed-mode chromatographic technique that exploits the unique properties of hydroxyapatite (Ca10(PO4)6(OH)2), utilizing both cation-exchange interactions via its phosphate groups and metal affinity through its calcium sites to achieve highly selective protein separations.</p><p>Renowned for its efficacy in resolving complex biomolecular separations, HAC excels in tasks like aggregate removal from monoclonal antibodies, surpassing the capabilities of traditional single-mode chromatographic methods such as ion-exchange or hydrophobic interaction chromatography.</p><h5><strong>Hydrophobic Interaction Chromatography</strong></h5><p>Hydrophobic interaction chromatography (HIC) is a method of protein purification that separates molecules based on their hydrophobicity, using high-salt buffers to promote protein-ligand interactions while maintaining the protein's biological activity. The process involves gradually reducing the salt concentration to elute proteins in order to increase hydrophobicity, making it effective for purifying target molecules and removing impurities or aggregates, making it a valuable approach for intermediate and polishing steps during purification processes.</p><h5><strong>Affinity Chromatography</strong></h5><p>Affinity chromatography is a powerful and highly selective technique used to purify biomolecules based on specific, reversible interactions between a target molecule and an immobilized ligand. In many cases, recombinant proteins are engineered with affinity tags—such as His-tags or GST-tags—that facilitate binding to complementary ligands on the chromatography matrix.</p><p>This method offers the advantage of achieving several thousand-fold purifications in a single step while preserving the biological activity of the protein. It is widely employed for purifying enzymes, antibodies, and antigens, using a variety of ligands including specific antibodies, enzymes, and multifunctional compounds like concanavalin A and triazine dyes.</p></div></div></div></div>

Exploring Protein Purification Methods: Essential Techniques for Accurate Results

<h3><span><strong>A Practical Guide to Getting the Most from Syringe Filters: Tips, Tricks, and Common Pitfalls</strong></span></h3><p><span><strong>Syringe filters play a crucial role in chromatography and many other laboratory applications by removing particulate matter before analysis. Proper sample filtration helps protect analytical instruments, extends column lifetime, and improves data quality. However, selecting an unsuitable filter—or using one incorrectly—can introduce contamination, reduce filtration efficiency, or even compromise analytical results. The following recommendations will help you avoid common mistakes and ensure reliable filtration every time.</strong></span></p><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/KNAUER_Avoiding_Common_Syringe_Filter_Mistakes_3_150e8c1c2b.webp" alt="KNAUER: Avoiding Common Syringe Filter Mistakes" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_KNAUER_Avoiding_Common_Syringe_Filter_Mistakes_3_150e8c1c2b.webp 133w," sizes="100vw" width="1276" height="1496"></p><h4><span><strong>1. Improper Filter Handling</strong></span></h4><p><span>Using excessive force during filtration or attempting to reuse disposable syringe filters can damage the membrane and increase the risk of sample contamination.</span></p><ul><li><span><strong>Solution:</strong> Apply slow, even pressure throughout the filtration process and always dispose of single-use filters after one use. If the flow rate decreases noticeably, replace the filter instead of applying additional force.</span></li></ul><h4><span><strong>2. Selecting an Inappropriate Pore Size</strong></span></h4><p><span>Using the wrong pore size may either fail to remove unwanted particles or cause the membrane to clog prematurely, reducing filtration efficiency.</span></p><ul><li><span><strong>Solution:</strong> A 0.45 µm membrane is generally suitable for routine particulate removal, while a 0.22 µm membrane is recommended for finer filtration and bacterial removal. Samples with higher viscosity may require a pre-filter to prevent rapid clogging. It is also important to consider the intended analytical technique. For example, 0.22 µm filters are typically preferred for UHPLC and LC-MS applications, whereas 0.45 µm filters are often sufficient for conventional HPLC analyses.</span></li></ul><h4><span><strong>3. Choosing the Wrong Membrane Material</strong></span></h4><p><span>Different membrane materials exhibit different chemical resistance. Using an incompatible membrane may lead to degradation of the filter, sample contamination, or complete filtration failure.</span></p><ul><li><span><strong>Solution:</strong> Always verify solvent compatibility before filtration. PTFE membranes are an excellent choice for organic solvents, while PES and nylon membranes are better suited to aqueous samples. If compatibility is uncertain, perform a small-scale trial before filtering valuable samples.</span></li><li><span><strong>Pro Tip:</strong> For additional guidance, see our blog article <strong>"How to Choose the Right Membrane for your Syringe Filter"</strong>, which provides a detailed comparison of membrane materials and their recommended applications.</span></li></ul><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/KNAUER_Avoiding_Common_Syringe_Filter_Mistakes_2_e47021fb11.jpg" alt="KNAUER Sepapure® Syringe Filters are available with multiple membrane materials, each identified by a dedicated color code for quick and reliable selection." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_KNAUER_Avoiding_Common_Syringe_Filter_Mistakes_2_e47021fb11.jpg 245w," sizes="100vw" width="1580" height="902"></p><h4><span><strong>4. Ignoring Filter Capacity</strong></span></h4><p><span>Using a filter with insufficient capacity can result in rapid membrane blockage, increasing filtration time and reducing laboratory productivity.</span></p><ul><li><span><strong>Solution:</strong> When filtering larger sample volumes, choose high-capacity syringe filters or introduce a pre-filtration step. Never force liquid through a clogged membrane, as doing so may damage the filter and negatively affect sample quality.</span></li></ul><h4><span><strong>5. Overlooking Sample Adsorption</strong></span></h4><p><span>Certain membrane materials may adsorb analytes of interest, leading to lower recoveries and potentially affecting quantitative results.</span></p><ul><li><span><strong>Solution:</strong> For protein samples or other adsorption-sensitive analytes, select membranes with low binding characteristics, such as nylon or glass fiber, whenever appropriate. Performing a small recovery test before routine analysis can help identify potential analyte losses.</span></li></ul><h4><span><strong>6. Failing to Optimize Filtration Performance</strong></span></h4><p><span>Slow filtration is more than just an inconvenience—it often indicates that the filtration conditions are not optimal.</span></p><ul><li><span><strong>Solution:</strong> If filtration becomes unusually slow, investigate possible causes such as membrane clogging, excessive sample viscosity, or an unnecessarily small pore size. Depending on the sample, dilution or sequential filtration using progressively finer filters may significantly improve performance.</span></li></ul><h4><span><strong>Final Thoughts</strong></span></h4><p><span>Selecting the appropriate syringe filter is a straightforward process, but overlooking small details can have a significant impact on analytical performance. Choosing the correct membrane material, pore size, and filtration strategy helps improve laboratory efficiency, protects chromatography systems and columns, and contributes to accurate, reproducible analytical results.</span></p><p><span>With the right syringe filter and proper filtration technique, you can minimize sample preparation problems and maximize confidence in every analysis.</span></p><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/KNAUER_Avoiding_Common_Syringe_Filter_Mistakes_4_983a15e289.jpg" alt="KNAUER: Sepapure® Syringe Filter Selection" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_KNAUER_Avoiding_Common_Syringe_Filter_Mistakes_4_983a15e289.jpg 245w," sizes="100vw" width="1160" height="627"></p><p><span><strong>Happy filtering!</strong></span></p>

Avoiding Common Syringe Filter Mistakes

News from LabRulezLCMS Library - Week 27, 2026

Article

Product

Application

Academy

Poster

Everything from the world of analytical chemistry in one place. We connect people in solving their problems. At Labrulez you will find all the necessary information easily, quickly and clearly. Stop searching and start finding.

LabRulez

Phenomenex is a global technology leader committed to developing novel analytical chemistry solutions that solve the separation and purification challenges of researchers in industrial, government and academic laboratories.

Phenomenex

Based in Berlin, KNAUER is a medium-sized, owner-managed company that has been serving the sciences since 1962. We develop and manufacture scientific instruments of superior quality for liquid chromatography, including: Analytical HPLC/UHPLC, Preparative HPLC, Fast protein liquid chromatography (FPLC), Multi-column chromatography/Simulated moving bed (SMB) chromatography. High pressure dosing, in-line detection systems, and osmometry are additional business areas.

KNAUER

Tosoh Bioscience

Agilent Technologies

MAC-MOD ANALYTICAL

Thermo Fisher Scientific

Shimadzu Corporation

SCIEX

<p><strong>In this part of the course we will deal with sample preparation: why, how and which sample preparation is the most suitable for the target analyte?</strong></p><p>SPE, LLSE, or syringe filter?&nbsp;</p><blockquote><p><strong>Presenter:</strong> Shaun Pritchard (Product Specialist Liquid Phase Separations, Agilent Technologies)</p><p>Shaun Pritchard is a Product Specialist for separation products at Agilent Technologies covering the UK and Ireland. He started his career in 1989 working at a large Pharmaceutical company holding a number of roles, this is where he became passionate about HPLC finally in 1998 he took the leap to leave the lab and move to the commercial side of things and has had a number of roles within the industry until 2017 he joined Agilent.</p></blockquote>

Agilent Technologies: HPLC Beginner Webinar Series

Sample Preparation in HPLC

Why, how and which sample preparation is the most suitable for the target analyte?

<p><strong>Tailing, fronting, peak doubling are all topics that we will cover during troubleshooting.</strong></p><p>After the session, we will be able to identify causes and avoid errors.</p><blockquote><p><strong>Presenter:</strong> Giorgio Ferlat (MSc, EMEAI IDO Product Specialist, Chemistries and Supplies, Agilent Technologies)</p><p>Giorgio achieved his Master Degree in Chemistry at the University of Trieste, Italy and has over 25 years of analytical chemistry experience, both in the laboratory and in the field, with strong expertise.</p><p>He has over 15 years of experience in consultative consumable sales and business development covering both national and international positions.</p></blockquote>

Troubleshooting and Everyday Routine (HPLC Columns)

Tailing, fronting, peak doubling are all topics that we will cover during troubleshooting. 

<p><span data-bind="expander:{'expandText': moreButtonText(), 'userCollapseText': lessButtonText(), 'text':getWebinarInfo().description}"><strong>With an understanding of selectivity, and using a variety of chromatographic data, we discuss phase design principles and how it is possible to introduce functionality to enhance selectivity through different mechanisms such as hydrophobicity, π-π, dipole-dipole and shape selectivity interactions.</strong></span></p><p><span data-bind="expander:{'expandText': moreButtonText(), 'userCollapseText': lessButtonText(), 'text':getWebinarInfo().description}">This webinar is the second part in a three-part series by Gemma Lo, called "A Systematic UHPLC / HPLC Method Development Strategy with Complementary Stationary Phases to Maximize Selectivity and Resolution."</span></p><p><span data-bind="expander:{'expandText': moreButtonText(), 'userCollapseText': lessButtonText(), 'text':getWebinarInfo().description}">Each part is about 20min long.</span></p><p><span data-bind="expander:{'expandText': moreButtonText(), 'userCollapseText': lessButtonText(), 'text':getWebinarInfo().description}"><strong>Presenter:</strong> Gemma Lo</span></p>

MAC MOD Analytical: Utilizing Stationary Phase Design in Method Development

Utilizing Stationary Phase Design in Method Development

We discuss phase design principles and how it is possible to introduce functionality to enhance selectivity through different mechanisms.

<p><span><strong>Overview:</strong></span></p><ul><li>Automated clean-up using solid-phase extraction for per- and polyfluorinated alkyl substances (PFAS) (presented by Norina Aßhoff/CVUA-MEL).</li><li>PFAS method according to LOD / LOQ limits in Recommendation (EU) 2022/1431 on the monitoring of perfluoroalkyl substances in food.</li><li>In fruit, vegetables &amp; baby food: limits for PFOS: 2 ng/kg, PFOA: 1 ng/kg, PFNA: 1 ng/kg, and PFHxS: 4 ng/kg.</li><li>Validated method for animal-based foods such as eggs, milk, meat and fish.</li><li>Suitability for PFAS analysis of food contact materials made of paper.</li><li>2-SPE cartridge setup (Strata GCB 250mg + Strata-X-AW 100mg) vs. single/stacked cartridge Strata PFAS (250mg / 100mg).</li></ul><p><span><strong>Key Learning Points:</strong></span></p><ul><li>Automated sample preparation of PFAS in food, food contact materials and consumer goods.</li><li>Additional sample preparation and LC-MS/MS methods for water, soil, and blood/serum.</li><li>Blank value-reduced SPE materials and other specific laboratory accessories for PFAS analysis.</li><li>Optimization of LC-MS/MS analysis through modern columns and strategies for better retention of short-chain PFAS.</li></ul><p><span><strong>Who should attend?</strong></span></p><p>The webinar is designed for all involved in PFAS analysis in the food, feed, environmental and clinical areas, to learn about automation and new ideas for more robust PFAS metho<strong>ds.</strong></p><blockquote><p><span><strong>Presenter:</strong></span> Norina Aßhoff, CVUA-MEL</p><p><span><strong>Presenter:</strong></span> Guido Rimmel, Account Manager, Phenomenex</p><p><span><strong>Presenter:</strong></span> Georg Pfefferer, Account Manager, Phenomenex</p></blockquote><p>Please note: the webinar on PFAS will not be recorded.</p>

Phenomenex: PFAS Analysis in Food, Vegetables and Other Matrices – Automation, Hints & Developments

PFAS Analysis in Food, Vegetables and Other Matrices – Automation, Hints & Developments

Learn about automation and new ideas for more robust PFAS methods. 

PFAS Analysis in Food, Vegetables and Other Matrices

<p><strong>Gain insights into how delay columns, essential to control the presence of PFAS in the laboratory, function. And understand how different delay columns impact method performance.</strong></p><p>We will host a Q&amp;A session after the presentation to address any questions or comments.</p><blockquote><p><strong>Presenter:</strong> Landon Wiest, PhD (Product Manager - LCMS, Shimadzu Scientific Instruments)</p><p>Dr. Landon Wiest is the Product Manager for LCMS at Shimadzu Scientific Instruments, leading a team of scientists who focus on applications development customer care. He has extensive experience in method and product development, research project management, and customer applications, with a strong background in liquid chromatography. At Restek Corporation, Landon led technology development teams and advanced new particle technologies, while also publishing application notes and creating educational content for webinars and short courses.</p><p>He earned a Ph.D. in Analytical Chemistry from Brigham Young University, where he developed patented diamond/carbon-based chromatographic particles. Recognized for his contributions to chromatography, Landon was nominated for the Csaba Horváth Award. Fluent in Danish and knowledgeable in German, Landon excels at communicating complex scientific concepts and is dedicated to advancing scientific knowledge.<br><br><strong>Presenter:</strong> Ruth Marfil-Vega, PhD (Sr Market Manager – Environmental, Shimadzu Scientific Instruments)</p><p>Dr. Ruth Marfil-Vega has 20 years of expertise in environmental chemistry and engineering in the US and Europe. She has worked with the EPA and the utilities industry to design and execute comprehensive solutions for addressing new environmental and water quality issues, including PFAS, regulated and unregulated DBPs, and other emerging contaminants. Since Ruth joined Shimadzu five years ago, she has continued to work with environmental stakeholders to ensure that analytical instrumentation and workflows support their success. She earned a BSc in Chemistry from the University of Valladolid (Spain) and a PhD in Environmental Science and Engineering from the University of Cincinnati.</p></blockquote><h3><strong>PFAS in Focus</strong></h3><p><strong>Welcome to PFAS in Focus, a comprehensive and exclusive webinar series sponsored by Shimadzu, covering the basics and beyond about the analysis of Per- and Polyfluoroalkyl Substances (PFAS) in the environment.</strong></p><p>Join our experts, live or on-demand after the original air date, to demystify the challenges of PFAS analysis and gain the insights you need to:</p><ul><li>Manage PFAS contamination in your laboratory.</li><li>Master standardized methods for PFAS testing.</li><li>Discover new analytical workflows to maximize the performance of your LC-MS/MS for PFAS analysis.</li></ul><p>Who should attend:</p><ul><li>Environmental industry professionals and academics currently working on PFAS analysis or looking to establish the application in their labs.</li><li>Representatives from drinking and wastewater utilities.</li><li>LC–MS users from any field interested in expanding their applications to include PFAS testing.</li></ul>

Mastering PFAS Control: Transform Your Laboratory Practices

Gain insights into how delay columns, essential to control the presence of PFAS in the laboratory, function.

<p><strong>Innovating for the future with cutting-edge solutions</strong></p><p><strong>Given the speed and scale of progress with Artificial Intelligence (AI) in today’s world, researchers and professionals across various scientific fields from the Danaher group of companies have stepped up in the game of AI and advanced technology to seek breakthrough solutions to age-old problems.</strong></p><h3><strong>Agenda</strong></h3><h5><strong>Introduction to Danaher</strong></h5><h5><strong>3D biology and the paradigm shift in next generation drug discovery</strong></h5><ul><li>Presented by Molecular Device</li></ul><h5><strong>AIVIA - introduction to AI based image analysis</strong></h5><ul><li>Presented by Leica Microsystems</li></ul><h5><strong>Analytical advances in the characterization of mRNA</strong></h5><ul><li>Presented by Phenomenex</li></ul><h5><strong>Unlocking the power of data with IDBS</strong></h5><ul><li>Presented by IDBS</li></ul><h5><strong>The power of AI: from planning and the lab to success in life sciences</strong></h5><ul><li>Presented by Abcam</li></ul><h5><strong>Practical approaches to EV characterization and purification: leveraging nano Flow Cytometry and ultracentrifugation for clinical and commercial success</strong></h5><ul><li>Presented by Beckman Coulter Life Sciences</li></ul><h5><strong>AI-MS/MS-AI mass spectrometry application with AI</strong></h5><ul><li>Presented by SCIEX</li></ul><h5><strong>Work smarter, not harder: emerging lessons on data in biopharma R&amp;D</strong></h5><ul><li>Presented by Genedata</li></ul><h5><strong>In silico process development with mechanistic chromatography modelling</strong></h5><ul><li>Presented by Cytiva</li></ul><h5><strong>CRISPR meets NGS: the powerful duo for drug discovery</strong></h5><ul><li>Presented by IDT</li></ul><h3><strong>Presenter:</strong></h3><ul><li>Gokhale, Aarti Arun, Ph.D (Sr Manager, Gene Reading, APAC, IDT)</li><li>Teo Chong Kok Ph.D (Scientific Account Manager, Genedata)</li><li>Edward Lim (General Manager, SEA, Beckman Coulter Life Sciences)</li><li>Taryn Guinan, Ph.D (Sr Applications Specialist, ANZ, Leica Microsystem)</li><li>Edward Wong, Ph.D (Sr Manager, Gene Writing &amp; Editing, APAC, IDT)</li><li>Stephan Waldert, Ph.D (Director, Data Science &amp; AI, Abcam)</li><li>Manish Manakchand (Commercial Director, Asia, IDBS)</li><li>Namrata Saxena (Technical Manager, Phenomenex)</li><li>Hans Lin (Field Application &amp; Market Development Specialist, SCIEX)</li><li>Mok Wei Chuan (Go Silico Sales Specialist, APAC, Cytiva)</li><li>Minho Tak, Ph.D (Sr Application Scientist, JAPAC, Molecular Device)</li></ul>

SCIEX: AI and technology advancements from Danaher

AI and technology advancements from Danaher

In this live webinar, let’s explore how Danaher group of companies leverage AI and technologies in providing advanced solutions.

<p><span name="summary"><strong>In this educational back-to-school webinar, James Grinias (University of Rowan) and Frank Steiner (Thermo Scientific) discuss strategic approaches for development, optimization and transfer of legacy and modern HPLC methods, utilizing gradient elution.</strong></span></p><p><span name="summary">Discussion topics and data on how to more easily move to core-shell column technology, how to modify gradient methods effectively, and how to work within the framework of evolving rules for method transfer such as USP 621.</span></p><p><span name="summary">We also discuss using the right tool to reduce the impact of method transfer across HPLC platforms. &nbsp;</span></p><p><span name="summary">Key learning objectives:</span></p><ul><li><span name="summary">&nbsp;Understanding gradient-based LC separations</span></li><li><span name="summary">Scalable parameters in pharmacopeia method modernization</span></li><li><span name="summary">&nbsp;Tricks and tips on method development and transfer</span></li></ul><p><span name="summary">If you are unable to attend, please register to ensure you will receive the link to the recording.</span></p><blockquote><p><strong>Presenter:</strong> James Grinias (Associate Professor, Department of Chemistry &amp; Biochemistry, Rowan University)</p><p>James P. Grinias, Associate Professor, Rowan University, teaches analytical and bioanalytical chemistry. His research focuses on UHPLC columns and instrument miniaturization. His work has been recognized with recognition from the American Chemical Society Subdivision on Chromatography and Separations Chemistry, LCGC magazine, and The Analytical Scientist magazine.<br><br><strong>Presenter:</strong> Frank Steiner (Sr. Manager of Product Applications and Scientific Advisor, Chromatography and Mass Spectrometry, Analytical Instrument, Thermo Fisher Scientific)</p><p>Dr. Frank Steiner is Sr. Manager of Product Applications and Scientific Advisor in the HPLC organization of Thermo Fisher Scientific and coordinates scientific collaborations with external partners to advance UHPLC technologies and applications.</p></blockquote>

Thermo Fisher Scientific: The real impact of HPLC and column parameters

The real impact of HPLC and column parameters

We will discuss strategic approaches for development, optimization and transfer of legacy and modern HPLC methods, utilizing gradient elution.

<p><strong>Choosing the appropriate column for your polymer analysis can be a challenge. Gel Permeation Chromatography (GPC) and Size Exclusion Chromatography (SEC) are separation techniques often used for polymers to determine the molecular weight and molecular weight distribution. &nbsp;Whether working with organic or aqueous mobile phase conditions, there are numerous factors and criteria to consider beyond the MW range when selecting the ideal GPC or SEC column.</strong></p><p>These include:</p><ul><li>individual pore, MIXED, and multiporous packing features and benefits</li><li>particle and pore size considerations&nbsp;</li><li>column chemistries and polymer sample types</li></ul><p><br>Additionally, we will cover best practices for selecting solvents, choosing calibration standards, and for using columns together in series. Application examples will be given. With a diverse range of column options available, we will demonstrate how to maximize performance through your GPC/SEC column selection</p><blockquote><p><strong>Presenter:</strong> Jean Lane (Application Engineer, Agilent Technologies, Inc.)</p><p>Jean has been employed with Agilent since Nov 2010 in technical and applications support and has a primary role supporting Agilent’s full line of LC columns and consumables, which also includes support for Agilent’s GPC/SEC product line. She came to Agilent thru acquisition of Varian and the former Polymer Laboratories. Jean has 20 plus years of technical and applications support for both HPLC and GPC consumable products. In addition to practical chromatography experience in the laboratory, she has had many, many years in her technical role involving customer support providing assistance and troubleshooting for customer chromatography issues.</p></blockquote>

Agilent Technologies: Becoming a Better Chromatographer - Educational Webinar Series

Optimize Your Polymer Analysis by Selecting the Ideal GPC/SEC Column

GPC & SEC are separation techniques often used for polymers to determine the molecular weight and molecular weight distribution.

Polymer Analysis by Selecting the Ideal GPC/SEC Column

<p><strong>Protein-based therapeutics such as monoclonal antibodies are widely developed and used by the biopharmaceutical industry; in 2023, mAb-based therapeutics represented over half of the Top Ten best-selling drugs of the year, globally, and are one of the fastest growing modalities. However, these large molecules pose unique challenges in relation to sample preparation and analytical characterisation, making robust and fit-for-purpose methods essential to achieve high quality results.</strong></p><p>This December, we invite you to connect with fellow scientists to learn more about the latest innovations in Protein analysis at our ProteinConnect Virtual Forum. Through a selection of complementary presentations and resources, our specialists will share insights into the present and future of protein characterisation, including:</p><ul><li>How workflow solutions can address mAb CQA analysis</li><li>Hints and tips for optimising protein bioseparations</li><li>The latest tools and technologies to enhance your protein analysis</li><li>Novel approaches to support online analysis and production of mAbs</li><li>A look to the future – emerging trends in Biopharma analysis</li><li>And much more! agilent ms</li></ul><h5><strong>Agenda 10 December 2024</strong></h5><p>10:30 a.m. Approaching The Analysis Of mAbsAnd Protein-based Therapeutics: An Introduction</p><p>11:15 a.m. Optimizing Protein Therapeutic Analysis with Agilent Columns: Innovations And Applications</p><p>12:15 p.m. Online LC: A Powerful Tool For Process Monitoring During The Production Of mAbs</p><p>13:00 p.m. ProteoAnalyzer: Analysis of NIST mAbReference Material By Parallel SDS-Capillary Electrophoresis</p>

Agilent Technologies: ProteinConnect: Linking Science, Innovation and Therapeutics

ProteinConnect: Linking Science, Innovation and Therapeutics (Day 1)

We invite you to connect with fellow scientists to learn more about the latest innovations in Protein analysis at our ProteinConnect Virtual Forum.

ProteinConnect: Linking Science, Innovation and Therapeutics

<p><strong>Christian Rohrer, Director of Sales &amp; Support – EMEA at Tosoh Bioscience GmbH, will present the next edition of Tosoh Tuesday, a webinar series held by Chromatography Experts.</strong></p><p>On December 10, Sebastian Thuermann, Product Manager MCC, and Carsten-Rene Arlt, Application Scientist, Process Instruments at Tosoh Bioscience, will demonstrate that multi-column chromatography (MCC), including continuous capture and simulated moving bed (SMB) chromatography, offer cost-effective alternatives with higher productivity and lower resource consumption for pharmaceutical manufacturing.</p><p>After the webinar, ask your questions during the live Q&amp;A session.</p><blockquote><p><strong>Presenter:</strong> Sebastian Thuermann</p><p><strong>Presenter: </strong>Carsten-René Arlt</p><p><strong>Presenter:&nbsp;</strong>Christian Rohrer</p></blockquote>

Tosoh Bioscience: Tosoh Tuesday - Continuous Chromatographic mAb Polishing via Step Gradient-SMB

Tosoh Tuesday - Continuous Chromatographic mAb Polishing via Step Gradient-SMB

MCC and SMB chromatography, offer cost-effective alternatives with higher productivity and lower resource consumption for pharmaceutical manufacturing

LCMS webinars focusing on Consumables - page 11