News from LabRulezLCMS Library - Week 15, 2026

Traditional protein purification workflows in many laboratories still rely on ultracentrifugation and long incubation steps. These approaches often involve multi-step protocols, extended processing times, and the use of specialized equipment. As a result, workflows can become complex, time-consuming, and prone to variability.Magnetic bead-based protein purification offers a faster, simpler, and more efficient alternative by enabling selective capture and rapid magnetic separation.<h3>Limitations of Conventional Protein Cleanup Methods</h3>In standard laboratory workflows, protein cleanup typically involves:<ul><li>Multiple processing steps</li><li>Centrifugation and column equilibration</li><li>Dependence on specialized instrumentation</li><li>Long processing times</li></ul>These factors introduce several challenges:<ul><li>Increased complexity of workflows</li><li>Variability between runs, reducing reproducibility</li><li>Risk of sample loss during transfers, especially for small sample volumes or scarce targets</li><li>Limited throughput due to manual handling</li></ul><h3>Principle of Magnetic Bead-Based Purification</h3>Magnetic beads enable selective protein capture through surface functionalization.Each bead is coated with streptavidin, allowing the attachment of a biotinylated ligand. This ligand can be selected based on the target analyte and may include:<ul><li>Antibodies</li><li>Aptamers</li><li>Antigen fragments</li></ul>This functionalization step provides the beads with the specificity required to bind the target analyte.<h3>Materials and Key Components</h3><h4>Magnetic Beads</h4><ul><li>Streptavidin-coated surface</li><li>Functionalized with biotinylated ligands</li></ul><h3>Ligands</h3><ul><li>Selected for target specificity</li><li>Attached to beads prior to purification</li></ul><h3>Step-by-Step Procedure</h3><h4>1. Bead Activation</h4>Load the biotinylated ligand onto the streptavidin-coated magnetic beads to confer specificity.<h4>2. Washing</h4>Remove impurities after ligand loading to prepare the beads for interaction with the sample.<h4>3. Incubation with Sample</h4>Incubate the functionalized beads with the sample to allow binding between the ligand and the target analyte.<h4>4. Magnetic Separation</h4>Place the sample on a magnet:<ul><li>Magnetic beads (with bound analyte) are attracted to the vessel wall</li><li>The liquid phase can be separated easily</li></ul><h4>5. Washing</h4>Wash the beads while they remain immobilized by the magnetic field to remove:<ul><li>Weak interactions</li><li>Non-specific binding</li></ul><h4>6. Elution</h4>Add an elution solvent to release the purified analyte from the beads.<h3>Downstream Applications</h3>After elution, the purified sample is ready for further analysis, including:<ul><li>LC-MS</li><li>Western blot</li><li>ELISA</li><li>Activity assays</li></ul><h4>Advantages of Magnetic Bead-Based Workflows</h4>Magnetic bead-based purification offers several key benefits:<h4>Speed and Simplicity</h4><ul><li>No centrifugation required</li><li>No complex instrumentation needed</li></ul><h4>Improved Reproducibility</h4><ul><li>Consistent magnetic separation</li><li>Reduced variability between runs</li></ul><h4>Reduced Sample Loss</h4><ul><li>Fewer transfer steps</li><li>Suitable for small or scarce samples</li></ul><h4>Scalability and Throughput</h4><ul><li>Compatible with automation</li><li>Supports microplate formats:<ul><li>96-well plates</li><li>384-well plates</li></ul></li></ul><h3>Biozen™ Magnetic Beads Features</h3>Phenomenex Biozen™ magnetic beads incorporate several design features:<ul><li>Hydrophilic co-polymer surface<ul><li>Reduces non-specific hydrophobic interactions</li></ul></li><li>Biotinylated BSA spacer<ul><li>Acts as a linker to streptavidin</li><li>Helps orient capture ligands</li></ul></li><li>Monodispersed capture ligands<ul><li>Optimized binding capacity</li></ul></li></ul>These features contribute to:<ul><li>Reduced non-specific binding</li><li>Improved capture consistency</li><li>Cleaner eluates</li></ul><h3>When to Use Magnetic Beads</h3>Magnetic bead-based purification is particularly suitable when:<ul><li>Sample volumes are small</li><li>Fast turnaround is required</li><li>Minimal equipment is preferred</li><li>High-throughput workflows are needed</li></ul><h3>Conclusion</h3>Magnetic bead-based protein purification simplifies traditional workflows by eliminating centrifugation and reducing complexity. Through selective capture and efficient magnetic separation, it enables faster processing, improved reproducibility, and compatibility with high-throughput and automated systems.

Magnetic Bead-Based Protein Purification: A Faster and More Efficient Workflow

Microplastics and Nanomaterials: Understanding Emerging Contaminants in Environmental Testing

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The sample throughput of high-sensitivity liquid chromatography-mass spectrometry (LC-MS) based proteomics analysis is restricted by the pressure limitations of LC and column hardware, with some methods wasting as much as 50% of the method cycle time outside of MS acquisition.
Traditional routes for boosting throughput include shorter columns and gradients, higher flow rates, and using trap-and-elute workflows – all of which introduce trade-offs in sensitivity and proteome depth.
The Thermo Scientific™ Vanquish™ Neo tandem direct injection workflow leverages hardware innovations and software intelligence to eliminate method overhead while maintaining data quality across nano, capillary, and micro-flow LC-MS.
In this webinar experts from Evotec and Bayer will showcase how they implemented the tandem direct injection workflow for their proteomics work. Dr. Nagarjuna Nagaraj will present on how Evotec is using tandem direct injection with nano-LC columns packed in-house to boost sample throughput of their chemoproteomics screening pipeline, while Dr. Alexander Boychenko from Bayer will discuss how his lab is using a micro-flow tandem direct injection approach to analyze plasma samples for translational proteomics.
Learning Objectives
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<li>See how minimizing LC-MS method overhead can accelerate sample throughput while maintaining proteome depth
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<li>Discover how labs are using tandem direct injection for chemoproteomics and translational proteomics applications
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<li>Learn how to implement tandem direct injection in your proteomics workflow
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Presenter: Dr. Nagarjuna Nagaraj (Group Leader High Sensitivity Proteomics, Evotec)
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Dr. Nagarjuna Nagaraj is a proteomics group leader at Evotec focusing on high sensitivity and high throughput proteomics applications with more than 18 years of experience in proteomics and mass spectrometry. Nagarjuna obtained his PhD from the Matthias Mann lab focusing on sample preparation and MS data acquisition strategies for clinical applications. After a postdoc he directed the mass spectrometry core facility at the Max-Planck-Institute of Biochemistry working on a wide range of protein and peptide mass spectrometry projects such as bottom-up proteomics, structural mass spectrometry including HDX, native and crosslinking MS, intact protein and small molecule analysis.
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Presenter: Dr. Alexander Boychenko (Head of R&amp;D Laboratory Precision Medicine Markers, Bayer)
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Alexander Boychenko is leading Omics Research within the Translational Sciences team of the Pharmaceuticals division at Bayer Group. He is focused on implementing innovative analytical technologies and developing novel methodologies for proteomics, metabolomics, and ligand-binding assays (LBA) to comprehensively characterize human and animal model phenotypes, enabling disease understanding and novel biomarker discovery. He holds a Ph.D. in Analytical Chemistry from Kharkiv National University, Ukraine. During his post-doctoral work at the University of Groningen, The Netherlands, he combined LC-MS and immunoassays to discover and validate protein biomarkers in biofluids for cancer screening. Before joining Research and Early Development at Bayer AG, he developed and introduced new liquid chromatography-mass spectrometry products at Thermo Fisher Scientific. Alexander is a subject matter expert in LC-MS, biological data interpretation, and precision medicine. He utilizes his passion for applying technical expertise to develop new medicines.
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Presenter: Dr. Alec Valenta (Product Marketing Manager, Thermo Fisher Scientific)
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Alec is the product marketing manager for the Vanquish Neo UHPLC system and low-flow LC columns. Prior to joining Thermo Fisher Scientific he earned his PhD in analytical chemistry from the University of Michigan where he helped advance microdialysis sampling of neurochemicals and developed LC-MS methods to quantify small molecule neurotransmitters, neuropeptides, and metabolites in a variety of complex biological samples.
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Thermo Scientific: A Tandem LC Workflow for Maximizing Mass Spectrometer Utilization in Proteomics

A Tandem LC Workflow for Maximizing Mass Spectrometer Utilization in Proteomics

How experts from Evotec and Bayer implemented the tandem direct injection workflow for their proteomics work.

A Tandem LC Workflow for Maximizing Mass Spectrometer

Today, tomorrow, and forever – PFAS, the “forever chemical”, have emerged as one of the greatest public health concerns of our time, infiltrating our environment and food supply. Herein, join us on Digital Classrooms for an in-depth exploration of PFAS, tracing their origins in everyday consumer products and examining their potential health implications.Our experts, Dr Yap Siew Qi and Elton Goh, will guide you through this critical topic, exploring the regulatory landscape with insights into EFSA and FDA guidelines. They will also introduce Shimadzu's innovative LC-MS/MS solutions, featuring the latest LCMS-TQ RX series. Discover how their robustness, sensitivity, and accuracy can enable effective detection of PFAS in complex food and environmental samples.Let’s work together to overcome global PFAS challenges. Register now to stay informed on this pressing issue!<h5>Learning Objectives:</h5><ul><li>Understand the implications of PFAS presence in food and environment &nbsp;</li><li>Navigate the evolving regulatory landscape for PFAS &nbsp;</li><li>Discover the capabilities of Shimadzu LCMS-TQ RX to detect PFAS in food and environmental samples</li></ul><blockquote>Presenter: Goh Wan Yu Elton (Market Specialist Food, Shimadzu (Asia Pacific) Pte Ltd)Elton is a dedicated and experienced Food Market Specialist at Shimadzu, bringing over nine years of expertise in food safety and analytical chemistry to his role. His career began at the Singapore Food Regulatory Board, where he spent seven years honing his skills and developing a profound understanding of regulatory standards and testing protocols. With a passion for knowledge and innovation, Elton transitioned to the private sector, contributing significantly to the advancement of analytical techniques and the development of novel food safety solutions during his two-year tenure at a research and development centre.Now at Shimadzu, Elton continues to excel, leveraging his expertise to develop cutting-edge analytical methods for detecting and quantifying various compounds in food products. His commitment to food science is evident in his efforts to enhance safety standards, build consumer trust, and make meaningful contributions to the global food industry.Presenter:&nbsp;Yap Siew Qi (LCMS Product Specialist, Shimadzu (Asia Pacific) Pte Ltd)Yap Siew Qi is an experienced professional in analytical chemistry, with a strong focus on liquid chromatography-mass spectrometry (LCMS). Her career commenced as a Research Assistant at the NUS Life Sciences Institute, where she acquired substantial experience in scientific research and laboratory techniques. This role was pivotal in developing her comprehensive understanding of life sciences, setting the stage for her future contributions to analytical chemistry and instrumentation.In 2022, her journey continued at Shimadzu (Asia Pacific), where she joined as an Application Chemist, focusing on LC/LCMS. In this capacity, she refined her expertise in LCMS technology, delivering prompt application support and technical solutions for the customers. Her exceptional skills and knowledge in LCMS soon led to her advancement to the position of Product Specialist. In this role, she continues to provide specialized knowledge and support, demonstrating her dedication to the progression of analytical chemistry and her proficiency in applying innovative technology within the field.Presenter:&nbsp;Mr. Ramaswamy Parthiban (Assistant Vice President, TUV SUD South Asia)Mr. R Parthiban has a robust background in operations management and quality assurance within the testing, inspection, and certification industry. He currently serves as AVP at TUV SUD South Asia, a position he has held since March 2022. Prior to this, he was the National Head at Prime Certification &amp; Inspection from April 2020 to June 2021. His experience also includes a significant role as Director of Operations at ALS Testing Services India Pvt Ltd from July 2018 to March 2020, where he led strategic initiatives and operational excellence.Earlier in his career, Mr. Parthiban held leadership roles including VP Operations at Merieux NutriSciences and Laboratory Manager for Chemistry at Exova Bahrain/KSA. His extensive experience in the industry is complemented by his earlier positions as Senior Manager at Coca-Cola and Laboratory Manager at SGS India Private Ltd. He began his career as a Research Associate at the John F. Welch Technology Center and ITC R&amp;D Center, eventually progressing to an Executive role in Quality Assurance at Sanmar Speciality Chemicals Ltd. Mr. Parthiban's career is marked by his expertise in quality systems, operational management, and strategic leadership in both national and international contexts.</blockquote>

Shimadzu: Discover The Latest LC-MS/MS Innovations For PFAS Challenges

Discover The Latest LC-MS/MS Innovations For PFAS Challenges

Discover how their robustness, sensitivity, and accuracy can enable effective detection of PFAS in complex food and environmental samples.

Understanding disease at a biological level requires deep, precise analysis. Multi-omics assays provide this depth, offering critical insights into human health and disease progression. However, these advanced methods come with challenges—especially when it comes to standardization and reproducibility.<h4>Join us for an informative webinar and panel discussion where we will:</h4><ul><li>Explore Quantitative Metabolomics: Discover how this powerful approach distinguishes health from disease and unlocks new insights into biological mechanisms.&nbsp;</li><li>Learn About Automated Sample Preparation: Get up to speed on the latest advancements in automated workflows, including the use of kit-based LC-MS/MS for reliable metabolomics profiling.Enhance Your Research Efficiency: Understand how integrating advanced technologies can streamline the verification and monitoring of potential biomarkers, leading to more cohesive analytical workflows.</li></ul><h4>Featured Presentations</h4><ul><li>Hear from experts at the University of Minnesota and University of Surrey as they showcase real-world applications and validations of these workflows. You'll gain exclusive insights from leaders in the field who are at the forefront of metabolomics research.</li></ul><h4>Key Learning Points</h4><ul><li>Unlock the Power of Quantitative Metabolomics: Learn how this approach helps differentiate health from disease and provides crucial insights into biological processes.</li><li>Streamline Your Research: Discover how comprehensive analytical workflows can speed up the verification and monitoring of lipid and small molecule biomarkers.</li><li>Dive into Advanced Automation: Explore cutting-edge sample preparation with the Waters™ Andrew+ automated liquid handler and the biocrates MxP® Quant 500 kit, transforming targeted metabolomics studies.</li><li>Master LC-MS/MS Workflows: Gain insights into the expanded biocrates MxP® Quant 500 XL, optimized for use with the Waters Xevo™ TQ Absolute MS, for robust lipidomics and metabolomics data.</li><li>Engage with Leading Experts: Take advantage of the opportunity to interact with experts from the University of Minnesota, University of Surrey, Waters Corporation, and Biocrates.</li></ul><blockquote>Presenter: Dr Holly-May Lewis (Senior Laboratory Technician, University of Surrey, UK)Dr. Holly-May Lewis is a Senior Laboratory Technician and specialist in Liquid Chromatography Mass Spectrometry (UPLC-MS/MS) at the University of Surrey's Chronobiology Section. With a PhD in Chemistry from the University of Surrey, she brings deep expertise in analytical chemistry to her current work, where she focuses on cutting-edge UPLC-MS/MS metabolomics of human samples.Presenter:&nbsp;Professor Debra Skene PhD (Professor of Neuroendocrinology, University of Surrey, UK)Debra J. Skene, PhD, FMedSci is a distinguished Professor of Neuroendocrinology in the Chronobiology Section at the University of Surrey, UK. With over 30 years of research experience and more than 200 peer-reviewed publications, her work focuses on the human circadian timing system and related sleep/wake disorders, particularly in blind individuals, shift workers, and older adults. Currently, her research explores the links between circadian clocks, sleep, and metabolism in both health and disease, utilizing advanced metabolomics techniquesPresenter:&nbsp;Dr Zachary J Mayer (Research Scientist at the Center for Metabolomics and Proteomics (CMSP), University of Minnesota)Dr Zachary Mayer is a Research Scientist at the Center for Metabolomics and Proteomics (CMSP) at the University of Minnesota, specializing in high-throughput targeted metabolomics by Liquid Chromatography Mass Spectrometry (LC-MS), sample preparation, and laboratory automation.</blockquote>

Waters Corporation: LC-MS/MS Workflows for Kit Based Quantitative Metabolic Profiling

LC-MS/MS Workflows for Kit Based Quantitative Metabolic Profiling

Multi-omics assays provide this depth, offering critical insights into human health and disease progression.

LC-MS/MS Workflows for Kit Based Quantitative Metabolic

The presence of per- and polyfluoroalkyl substances (PFAS) in food supply chains is a growing concern due to their environmental persistence and potential health risks.As awareness increases, the demand for effective regulations and advanced analytical methods for detecting PFAS in food is critical. In this webinar hosted by Agilent in association with AOAC India Section, we will explore the current PFAS contamination landscape, regulatory frameworks, and the latest analytical solutions.The session will cover PFAS characteristics and the challenges they present to food safety. We will examine global and regional regulations, with a focus on evolving standards in India and the South Asia Pacific region. Also, the webinar will highlight advanced analytical approaches for different food matrices like seafood, eggs, meat, infant formula, packaging material, including sample preparation, instrumentation, and reporting essential for effective PFAS detection. Agilent's robust LC/MS and GC/MS PFAS testing solutions will be showcased along with the Captiva EMR sample preparation kit, demonstrating how they can help food manufacturers, exporters, and regulatory bodies ensure compliance with global standards.<h4>Learning outcomes:</h4><ul><li>Comprehensive understanding of PFAS characteristics and the regulatory landscape across global and regional markets, including India and the South Asia Pacific.</li><li>Advanced analytical techniques for PFAS detection in food, covering sample preparation, instrumentation, and reporting methodologies.</li><li>Practical insights into implementing PFAS testing solutions to ensure compliance with international food safety standards.</li></ul><h4>Who should attend:</h4><ul><li>Food scientists, analytical chemists, regulatory authorities, government lab personnel, industry professionals in food manufacturing, packaging, exporting, R&amp;D scientists, toxicologists, QA/QC analysts and managers, academicians, and researchers focused on PFAS analysis and food safety.</li></ul><blockquote>Presenter: Dr. Nick Birse (Lecturer, School of Biological Sciences, Queen's University, Belfast, Northern Ireland, UK)Nick Birse is a Lecturer in Mass Spectrometry at Queen’s University Belfast, where he focuses on the use of mass spectrometry to solve key food integrity challenges, such as the measurement of chemical contaminants and natural toxins, fingerprinting foods to determine geographic origin and help detect fraud, or simply to measure key quality, flavour and taste attributes, all of which makes foods tastier, healthier and safer for consumers. Nick previously served as a Research Fellow within Prof Chris Elliott’s research group, assisting in the execution of Chris’s 2021 Agilent Thought Leader Award. He holds a PhD in Analytical Chemistry from Queen’s University Belfast.Presenter:&nbsp;Dr. Niladri Sekhar Chatterjee (Senior Scientist and DST Inspire Fellow, ICAR-Central Institute of Fisheries Technology, India)Dr. Niladri has more than 10 years of experience in precision measurement of small molecules in food, biological, and environmental matrices. His doctoral research is on pesticide metabolism, and environmental fate in crop, soil, and water. His postdoctoral research is on seafood authentication using high resolution mass spectrometry-based metabolomics and molecular spectroscopy fingerprinting. He currently works as a Senior Scientist in the Biochemistry and Nutrition Division at ICAR Central Institute of Fisheries in Kochi. At CIFT, he has been instrumental in establishing a state-of-the-art analytical chemistry laboratory from scratch which is today one of the National Reference Laboratories. He has received external grants from the Science and Engineering Research Board (SERB) India, National Agricultural Science Fund, and FSSAI. In the NRL, he has developed and validated precision measurement methods for multiclass analysis of pesticides, antibiotic residues, and endocrine disruptors and is actively managing the ISO 17025 accreditation of the laboratory. His research work has resulted in several peer reviewed publications.Presenter:&nbsp;Dr. Lorna De-Leoz (Global Food Segment Director, Agilent Technologies, USA)Lorna De Leoz has nearly 20 years of experience in the food industry. As the Global Food Segment Director, she collaborates with scientists to help develop accurate and reliable testing for food safety, quality, and authenticity. Lorna worked at the National Institute of Standards and Technology in the Mass Spectrometry Data Center for seven years. She completed her Ph.D. in Chemistry at University of California, Davis on milk oligosaccharides and glycan biomarkers analyses.</blockquote>

Agilent Technologies: PFAS in Food Supply Chains: Regulations and Analytical Approaches: in association with AOAC India Section

PFAS in Food Supply Chains: Regulations and Analytical Approaches: in association with AOAC India Section

PFAS characteristics and the challenges they present to food safety.

PFAS in Food Supply Chains: Regulations and Analytical

Day one: Innovation and data insights<ul><li>Discover how to achieve high sensitivity, speed, and resilience when working with large, complex sample sets</li><li>Learn strategies to speed up your covalent protein drug discovery</li><li>Gain insights into accelerating the development of multiple modalities</li><li>Understand the latest advancements in quantitative proteomics with ZT Scan DIA</li><li>Overcome limitations with cutting-edge artificial intelligence quantitation software</li></ul>Agenda-at-a-glanceWelcome and introduction to innovation and data insights&nbsp;&nbsp;&nbsp;&nbsp;Advancing robustness and serviceability of a high-end triple quad mass spectrometer in a high-throughput bioanalytical lab<ul><li>Highly sensitive mass spectrometers allow the advanced bioanalytical labs of today to achieve the LOQ required of potent biologic drugs and provide maximum flexibility in choice of sample prep techniques. However robust mass spectrometers are crucial for several reasons:<ul><li>Aid in ensuring the consistent quantitative performance of an assay</li><li>Effectively allow a lab to meet their project timelines</li><li>Are essential for handling the inherent variability of analyzing diverse molecules and diverse samples required for bioanalysis</li></ul></li></ul>The new fast in drug discovery: a new MS system to study covalent protein drugs<ul><li>The combination of a super-fast sampling interface with the highly sensitive ZenoTOF 7600 system makes it an ideal system for evaluating covalent binding screens. Main findings in our experiments using the Echo® MS+ system:<ul><li>Speed: measurements from a full 384-well plate in 15 min or 1536-well plate in less than one hour</li><li>Precision: low injection volume supports repetitive measurements and time series; capture full kinetic data set in one experiment</li><li>Quality: high data quality allows for full automated processing, delivering immediate hitlist output</li></ul></li></ul>icIEF-UV/MS characterization of charge Isoforms for biotherapeutic products<ul><li>Characterization of icIEF charge isoforms, whether in early product development or associated with licensure filings, is usually achieved through ion exchange (IEX) liquid chromatography coupled with mass spectrometry. icIEF-MS technology has recently emerged to enable the direct characterization of specific species in the icIEF electropherogram, eliminating the need for extensive method development.<ul><li>Discover opportunities to advance core platform technologies with novel characterization approaches</li><li>Hear case studies on understanding molecular structure information</li><li>New approaches to characterizing post-translational modifications of charge isoforms</li></ul></li></ul>Expanding qualitative and quantitative sensitivity for immunopeptidomics using ZT Scan DIA<ul><li>Learn how utilizing a research-grade Zeno-trap time-of-flight LC-MS system can advance current applications in immunopeptidomics and proteomics, including:<ul><li>What is ZT Scan DIA, the technology and how it benefits data acquisition</li><li>Preliminary proteomics and immunopeptidomics results using ZT Scan DIA</li><li>Future applications of immunopeptidomics using ZT Scan DIA</li></ul></li></ul>Artificial intelligence quantitation (AI quant): molecular structure and mass spectral data quality driven processing of high-resolution mass spectrometry for quantitative analysi<ul><li>High-resolution mass spectrometry (HRMS) has long sought to be a viable alternative for quantitative workflows but has been unable to broadly compete, mainly due to the lack of suitable data processing software. In this work we describe:<ul><li>A molecular structure-dependent workflow</li><li>The holistic and user-independent processesing of HRMS data</li><li>The automatic quantification of small drug-like molecules</li></ul></li></ul>Q&amp;A roundtable: innovation and data insights with the experts<ul><li>Moderated by: Jamie Wighton, Senior Director LC-MS Research, SCIEX</li><li>Panelists include:<ul><li>Rathna Veeramachaneni, Director, Biopharma LC-MS/MS, KCAS Bio</li><li>Xiaping He, Senior Scientist, Pfizer</li><li>Markus Stöckli, Senior Principal Scientist, Novartis Institutes for BioMedical Research</li><li>Tony Purcell, Professor, Monash University&nbsp;</li><li>Kevin Bateman, Retired Pharmacokinetics, Pharmacodynamics and Drug Metabolism Expert</li></ul></li></ul><blockquote>Moderator: Jamie Wighton (Senior Director LC-MS Research, SCIEX)Jamie joined SCIEX 20 years ago and has held multiple technical and leadership roles throughout his career at SCIEX. &nbsp;Starting out as an engineer in manufacturing operations, Jamie has since led teams in supply chain, product development, and most recently the research and product applications teams within R&amp;D. Prior to joining SCIEX, Jamie worked at JDS Uniphase as an optical test engineer.Jamie holds a B.Sc. in engineering physics from Queen’s University.Presenter: Rathna Veeramachaneni (Director, Biopharma LC-MS/MS, KCAS Bioanalytical)Presenter: Markus Stöckli, Senior Principal Scientist, Novartis Institutes for BioMedical ResearchPresenter: Xiaoping He, Senior Scientst, PfizerPresenter: Tony Purcell, Professor, Monash UniversityPresenter: Kevin Bateman, Retired Pharmacokinetics, Pharmacodynamics and Drug Metabolism Expert</blockquote>

SCIEX summit Day one: Innovation and data insights

Importance of LC-MS in food and environmental testing, pharmaceutical and biopharmaceutical discovery and development and biomedical and omics research.

Day two: Characterization and discovery<ul><li>Characterize and quantify calreticulin (CALR) arginylation using electron-activated top-down proteomics</li><li>Achieve in-depth profiling of metabolites and lipids in biological samples</li><li>Obtain accurate and reliable metabolite identification using electron-based fragmentation (EAD)</li><li>Detect trace amounts of pesticide residues in food by targeted and non-targeted, IDA and SWATH approaches</li><li>Enhance routine characterization of low-abundant sequence variant (SV) through fast and sensitive EAD-DDA</li></ul><h5>Agenda-at-a-glance</h5>Welcome and introduction to characterization and discoveryTop-down characterization of post-translational arginylation using electron activated dissociation (EAD)<ul><li>Learn about our top-down proteomics workflow for the characterization and quantification of calreticulin (CALR) arginylation. MS1 profile and MS2 fragmentation were characterized by EAD. Preferential fragmentation at the CALR N-terminals yielded sufficient c ions facilitating precise localization of the arginylation sites. Learn about:<ul><li>Arginylation regulates CALR degradation and ER function</li><li>EAD is efficient in characterizing N-terminal arginylation</li><li>The workflow sets up the foundation for unbiased top-down activity-based arginylation profiling</li></ul></li></ul>In-depth metabolomics and lipidomics analyses<ul><li>Routine measurement of metabolites and lipids has become a cornerstone of biomedical research. At the MPC, Helmholtz Munich, non-targeted analysis is based on the SCIEX ZenoTOF 7600 for accurate and in-depth analysis and annotation of metabolites and lipids from different biological specimens.<ul><li>Application of Zeno trap enabled non-targeted workflows to large scale metabolomics and lipidomics studies</li><li>Enhanced sensitivity enables deeper profiling of biological samples</li><li>Increased confidence in identifications through comprehensive structural elucidation using Electron activated dissociation (EAD)</li></ul></li></ul>MetID innovations in pharma: exploring new avenues for metabolite structure elucidationTime-of-flight mass spectrometry applications in food science: targeted and non-targeted approaches<ul><li>Time-of-flight mass spectrometry has become a key tool in analytical laboratories involved in food analyses. This instrumentation can be used for both targeted and non-targeted approaches. In this presentation, we will discuss:<ul><li>Overview of practical targeted and non-targeted approaches in food analysis</li><li>Application of a SWATH® approach to the analysis of pesticide residues in food</li><li>Application of IDA approaches in non-targeted food analyses</li></ul></li></ul>Reduce risk and accelerate cell line development with iCIEF-UV/MS technology<ul><li>Guide decisions during cell line development with the Intabio ZT system with more information on how process changes affect cell line and product stability. &nbsp;<ul><li>How to utilize icIEF-UV/MS to identify sequence variants</li><li>How this information can be used to create a mitigation strategy</li><li>What more information in less time means to groups throughout process development</li></ul></li></ul>Q&amp;A roundtable: A deeper look at high-resolution mass spectrometry<ul><li>Moderated by: ose Castro-Perez, Vice President of Product Management, SCIEX</li><li>Panelists include:<ul><li>Tom Lin, Instructor, Washington University in St. Louis</li><li>Michael Witting, Deputy Head Metabolomics and Proteomics Core and Executive Manager Metabolomics, Helmholtz Munich</li><li>Francisco José Díaz-Galiano, Postdoctoral researcher, University of Almería</li><li>Kirsten Nields, Principal Scientist, J&amp;J Innovative Medicines</li><li>Tatjana Talamantes, Senior Product Manager, Accurate Mass, SCIEX</li></ul></li></ul><blockquote>Moderator: Tatjana Talamantes, Senior Product Manager, Accurate Mass, SCIEXTatjana is a Senior Product Manager at SCIEX, overseeing accurate mass systems since 2021. She collaborates with R&amp;D, sales, and marketing teams to ensure effective project execution and portfolio management.Previously, Tatjana was a Product Manager at MOBILion Systems, leading the pre-commercialization of SLIM-based ion mobility instruments. She also has held roles at Thermo Fisher Scientific as a Sr. Vertical Marketing Specialist and Sr. Field Applications Scientist.Tatjana holds a B.A. in chemistry from Austin College and a Ph.D. in biomedical sciences and biochemistry from the University of North Texas Health Science Center.Jamie holds a B.Sc. in engineering physics from Queen’s University.Presenter: Tom Lin, Instructor, Washington University in St. LouisPresenter: Michael Witting, Deputy Head Metabolomics and Proteomics Core and Executive Manager Metabolomics, Helmholtz MunichPresenter: Francisco José Díaz-Galiano, Postdoctoral researcher, University of AlmeríaPresenter: Kirsten Nields, Principal Scientist, J&amp;J Innovative MedicinesPresenter: Tatjana Talamantes, Senior Product Manager, Accurate Mass, SCIEX</blockquote>

SCIEX summit Day two: Characterization and discovery

CIEX summit Day two: Characterization and discovery

<h4>The Waters 2024 Virtual Summer School - An Educational Journey to Master Food &amp; Environmental Analysis</h4>Unlock the full potential of LC-MS techniques with our immersive and educational sessions at the Waters Virtual F&amp;E Late Summer School 2024. From September 24th to 26th, engage with leading experts and discover cutting-edge methods for environmental and food analysis.<h5>Why attend?</h5><ul><li>Comprehensive Learning: Dive deep into advanced LC-MS techniques and gain practical knowledge to enhance your laboratory's analytical capabilities.</li><li>Expert Insights: Learn from industry leaders and gain firsthand experience through detailed modules on chromatography, mass spectrometry, and sample preparation.</li><li>Regulatory Compliance: Stay ahead with the latest insights on EU regulations for PFAS analysis in food and water, ensuring your lab meets stringent standards.</li></ul><h3>Episode #2: Tandem Quadrupole MS and MS-Method Development | F&amp;E Summer School - Day 1 - Module 2</h3>In this episode it’s all about the tandem quadrupole mass specs and the setup of MS-methods. We will demonstrate, using actual hardware, how simple it is to prevent contamination or clean a system, regardless of whether it’s at the low or high end of the sensitivity spectrum. You will learn valuable tips and tricks about UniSpray, a unique ion source and alternative to ESI.With our new waters_connect for Quantitation software, we will guide you through the different stages of MS-method development. You will learn how straightforward automated compound optimization can be, and how intuitively the new Method Acquisition Editor can be utilized by operators providing a clear structured all in one view of compounds and parameters. Search options make it easy to navigate through large multi-residue methods. And in the end, you are just one click away from the processing method that will be the topic of Episode 4.<h5>What you'll learn:</h5><ul><li>Easy operation and maintenance of Waters TQ-MS systems</li><li>Usage and benefits of UniSpray ion source</li><li>Step by step MS-method development with waters_connect for Quantitation</li></ul><blockquote>Presenter:&nbsp;Benjamin Wuyts, PhD (Senior Solution Support Specialist Europe, Waters Corporation)Benjamin initially joined Waters as an application chemist working in the Belgian demo lab, performing liquid chromatography-mass spectrometry demonstrations, support and training. In 2016 Benjamin became a European application chemist focusing on projects to support emerging applications. Benjamin has been focused over several years in establishing the Waters solution to the analysis of polar anionic pesticides, utilising novel column phases and LC-MS/MS technologies.</blockquote>

Waters Corporation: The Waters 2024 Virtual Summer School

Tandem Quadrupole MS and MS-Method Development

In this episode it’s all about the tandem quadrupole mass specs and the setup of MS-methods.

<h4>The Waters 2024 Virtual Summer School - An Educational Journey to Master Food &amp; Environmental Analysis</h4>Unlock the full potential of LC-MS techniques with our immersive and educational sessions at the Waters Virtual F&amp;E Late Summer School 2024. From September 24th to 26th, engage with leading experts and discover cutting-edge methods for environmental and food analysis.<h5>Why attend?</h5><ul><li>Comprehensive Learning: Dive deep into advanced LC-MS techniques and gain practical knowledge to enhance your laboratory's analytical capabilities.</li><li>Expert Insights: Learn from industry leaders and gain firsthand experience through detailed modules on chromatography, mass spectrometry, and sample preparation.</li><li>Regulatory Compliance: Stay ahead with the latest insights on EU regulations for PFAS analysis in food and water, ensuring your lab meets stringent standards.</li></ul><h3>Episode #4: Chromatography - APGC | F&amp;E Summer School - Day 1 - Module 4</h3>Discover the advantages of GC coupled to MS under atmospheric pressure (Atmospheric Pressure GC/ APGC) as a powerful alternative to electron ionization (EI) for GC-MS. Gain a comprehensive understanding of the APGC technology and its operational principles. Explore why APGC offers higher sensitivity, selectivity, and robustness compared to traditional EI methods. Maximize versatility and efficiency by understanding how to achieve both LC and GC capabilities on the same MS system as well as using nitrogen as an alternative carrier gas.<h5>What you'll learn:</h5><ul><li>How APGC works</li><li>Enhanced performance over classic EI-GC</li><li>Ease of use and implementation in the lab</li><li>Usage of nitrogen as GC carrier gas</li></ul><blockquote>Presenter:&nbsp;Frank L. Dorman, PhD, FRSC (Principal Environmental Market Manager, Waters Corporation)Frank Dorman is currently Senior Business Development Manager for Waters focusing on their global environmental business and, concurrently, Associate Professor (adjunct) in the Department of Biochemistry and Molecular Biology (BMB) at Penn State University in University Park, PA. In his role at Waters, Frank manages the research-focused environmental market, external collaborations and also interfaces with R&amp;D for new product development. Prior to Waters, Frank was employed full-time as a tenure-line Faculty member of Penn State and served as a member of the Graduate Faculty in: Biochemistry, Microbiology and Molecular Biology (BMMB), Chemistry (CHEM) and the Programs in Forensics Science (FRNSC), and Biogeochemistry (BGC). Frank is also a member of the Center for Molecular Toxicology and Carcinogenesis at Penn State. Frank’s scientific interests are in gas and liquid chromatography fundamental characterization, instrumentation and column development, as well and mass spectrometry and atomic spectroscopy as applied to trace analysis in complex sample matrices. This work is directed towards environmental forensics, traditional forensic chemistry and where environmental exposure events may contribute to human disease; an area sometimes referred to as “exposomics”.Prior to joining the faculty at PSU, Frank was at Restek Corporation for 14 years as the Director of Technical Development, and R&amp;D Senior Chemist. During most of this same time, Frank was also an Adjunct Research Professor at Juniata College where he maintained an active undergraduate research program. Prior to that he was at Inchcape Testing Services for 5 years as a Senior Chemist, Organics Section Manager for Sample Preparation and Corporate Methods Development Chemist. As such, Frank has extensive experience in both the Industrial and Academic sectors giving him a relatively rare combination of skills and experience.Presenter:&nbsp;Janitha De-Alwis (Senior Applications Chemist, European Applications Team, Food and Environmental, Waters Corporation)JD has worked in the chromatography industry for over 10 years and has recently joined Waters Corporation as a senior applications chemist. Prior to joining Waters, JD managed a group at a leading food research and contract organisation. Here, JD helped the food, drink and associated industriesproactivelyand reactively in the area of chemical toxins.As a senior applications chemist, JD now develops new applications with Waters technologies and helps customers with their critical analytical challenges.</blockquote>

Chromatography - APGC

Discover the advantages of GC coupled to MS under atmospheric pressure (APGC) as a powerful alternative to electron ionization (EI) for GC-MS.

<h4>The Waters 2024 Virtual Summer School - An Educational Journey to Master Food &amp; Environmental Analysis</h4>Unlock the full potential of LC-MS techniques with our immersive and educational sessions at the Waters Virtual F&amp;E Late Summer School 2024. From September 24th to 26th, engage with leading experts and discover cutting-edge methods for environmental and food analysis.<h5>Why attend?</h5><ul><li>Comprehensive Learning: Dive deep into advanced LC-MS techniques and gain practical knowledge to enhance your laboratory's analytical capabilities.</li><li>Expert Insights: Learn from industry leaders and gain firsthand experience through detailed modules on chromatography, mass spectrometry, and sample preparation.</li><li>Regulatory Compliance: Stay ahead with the latest insights on EU regulations for PFAS analysis in food and water, ensuring your lab meets stringent standards.</li></ul><h3>Episode #7: Quantification for Targeted Analysis | F&amp;E Summer School - Day 2 - Module 3</h3>MS Quan is an application in the waters_connect™ for Quantitation software platform that is designed to streamline the LC-MS/MS data processing and review with innovative workflows, data visualization and data navigation. The MS Quan application’s main workflows and overarching features such as task-oriented workflow, batch-level review, and exception-focused review are introduced and illustrated with a PFAS analysis dataset.<h5>What you'll learn:</h5><ul><li>How to create processing method</li><li>Data review with exception focus review</li><li>Compare data and evaluate peak integration</li><li>Export and report of final results</li></ul><blockquote>Presenter:&nbsp;Helge Grunwald (Sr Sales Applications Chemist, Waters Corporation)Helge is part of the Customer Success team at Waters with a focus on LC-MS/MS application and sales support. He works in this role for more than 4 years and gained experience in previous roles as project manager at Intertek and method developer at Basilea Pharmaceutica after he finished his Master of Science in Life Science at FH Nordwestschweiz.</blockquote>

Quantification for Targeted Analysis

The MS Quan application’s main workflows and overarching features are introduced and illustrated with a PFAS analysis dataset. 

PFAS testing has become one of the most important topics facing food and environmental laboratories over the last two years.&nbsp;Phenomenex and SCIEX have worked with collaborators to overcome the significant challenges of such analyses, and now offer extremely sensitive and robust LC-MS/MS and sample preparation solutions, with methods and software which are ready to implement.<h4>Key Learning Points</h4><ul><li>Background to PFAS and their regulations</li><li>System Settings and requirements</li><li>Correct HPLC column choice</li><li>Sample Preparation strategies from various matrices</li><li>PFAS as ubiquitous contaminants - Inerting the system</li><li>A sensitive and robust method applicable to water and food sample</li><li>Some insight about designed for PFAS products</li></ul><h4>Who should attend</h4><ul><li>LC/MS or Sample Prep Users, working with several matrices that want to get tips on PFAS method development or on improving their method.</li></ul><blockquote>Presenter: Jianru Stahl-Zeng, Dr.&nbsp; (Senior Technical Marketing Manager EMEAI, SCIEX)Jianru has been part of SCIEX since 2001. She initially was part of our application Support team and covered not only proteomics but also small molecules applications. Jianru moved to technical marketing and focused on Food and Environmental applications.Since 2018, she is managing the EMEAI technical marketing team and projects. Jianru is also the Global Technical Leader for Food and &nbsp;Environmental application.Presenter:&nbsp;Duilio Romanello (Senior Technical Specialist, Phenomenex)Duilio has been working with Phenomenex since 2008. Since 2010 he has been Product Specialist for the GC and SPE lines and then Account Manager for the South of Italy. Since November 2023 he is covering the role of Senior Technical Specialist.He has acquired experience in the Food and Environmental sectors by collaborating with important companies in the industry and providing technical support for the development of method optimization, guidance on applications and troubleshooting, assistance in choosing columns for HPLC, Gas Chromatography (GC), SFC and SPE.</blockquote>

Phenomenex: Advanced strategies in LC/MS/MS for the clean-up and the analysis of per- and polyfluoroalkyl substances (PFAS) in various matrices

Advanced strategies in LC/MS/MS for the clean-up and the analysis of per- and polyfluoroalkyl substances (PFAS) in various matrices

Extremely sensitive and robust LC-MS/MS and sample preparation solutions, with methods and software which are ready to implement.

LCMS webinars focusing on LC/QQQ - page 14