<div><p><strong>Purifying the target protein is crucial for investigating its specific properties and interactions with biomolecules like DNA, RNA, and other proteins. A range of purification strategies is available, and the optimal approach depends on factors such as the required scale, throughput, and downstream application. In many cases, the process must be fine-tuned through empirical optimization to achieve the desired purity and yield.</strong></p></div><div><div><div><div><h3><strong>What is Protein Purification?</strong></h3><p>Protein purification is a critical process in biotechnology and life sciences, aimed at isolating a specific protein from complex biological mixtures such as cell lysates or tissue extracts. The goal is to obtain a highly purified protein sample, free from contaminants like other proteins, nucleic acids, and lipids, to enable accurate analysis of its structure, function, and interactions.</p><p>This process is especially vital in biotechnological areas inside immunology and microbiology. In <strong>immunology</strong>, it underpins the production of vaccines, therapeutic antibodies, and diagnostic assays by enabling the isolation of key immune proteins and antibodies. It also supports the investigation of cell signaling and immune response mechanisms. In <strong>microbiology</strong>, purified proteins are essential for studying pathogens, identifying disease-related antigens, and driving drug discovery efforts. Ultimately, protein purification is fundamental to the development of targeted vaccines, innovative diagnostics, and effective therapeutic strategies.</p></div></div></div></div><div><div><div><div><h3><strong>Types of Protein Purification Methods</strong></h3></div></div></div></div><div><p>There are several types of protein purification methods, each chosen based on the protein’s characteristics such as size, charge, solubility, and binding affinity. Below are some commonly used methods:</p></div><div><div><div><div><h4><strong>Non-Chromatographic Approaches</strong></h4></div></div><div><p>Non-chromatographic approaches use simpler physicochemical principles—such as solubility, molecular size, or diffusional properties—to separate and purify proteins. These techniques are often employed as initial purification or concentration steps before further refinement.</p><h5><strong>Precipitation Techniques</strong></h5><p>Protein precipitation is a widely used approach in purification workflows, typically relying on one of three main methods:</p><ul><li><strong>Organic solvent precipitation</strong> involves adding solvents like ethanol or acetone to lower the solution's dielectric constant. This promotes protein–protein interactions, reducing solubility and leading to aggregation and precipitation.</li><li><strong>Isoelectric point precipitation </strong>exploits the pH at which a protein carries no net charge. At this point, solubility is minimized, causing the protein to precipitate from solution.</li><li><strong>Nonionic polymer precipitation</strong> uses polymers such as dextran, polyethylene glycol (PEG), or NPEP to alter the solution’s physical properties. These agents create crowding effects that promote protein aggregation without directly binding to the proteins.</li></ul><h5><strong>Dialysis</strong></h5><p>Dialysis involves placing a protein solution inside a semi-permeable membrane that allows water and ions to pass while the protein remains inside, facilitating buffer exchange.This method is commonly used during protein expression and purification workflows for buffer exchange, desalting, and removal of unwanted small molecules such as imidazole, urea, or ammonium sulfate. The process helps maintain protein stability and activity by gradually equilibrating the sample with the desired buffer conditions.</p><h5><strong>Immunocapture (Magnetic Bead-Based Purification</strong></h5><p>Immunocapture uses antibodies immobilized on solid supports like magnetic beads to selectively bind and isolate a target protein. This method is highly specific and particularly useful for low-abundance targets or small-scale prep, such as:</p><ul><li>Pulling down proteins from complex mixtures</li><li>Capturing tagged recombinant proteins (e.g., FLAG, HA, or His tags)</li><li>Enrichment before western blotting, MS, or ELISA</li></ul><p>Magnetic beads offer speed, ease of use, and minimal sample loss, making them ideal for lab-scale workflows.</p><p><a target="_blank" rel="noopener noreferrer" href="https://www.phenomenex.com/products/biozenspe-sample-preparation-products/biozen-magbeads">Biozen MagBeads</a> provide a fast and efficient method for purifying, isolating, and cleaning up monoclonal antibodies (mAbs), proteins, and peptides. These paramagnetic beads feature a streptavidin-coated surface, enabling high-affinity binding in immuno-based workflows. Compared to traditional sample preparation techniques, magnetic bead-based immunocapture offers greater speed and consistency by delivering high binding capacity with uniform particle size, ensuring accurate and reproducible results in significantly less time.</p><h5><strong>Centrifugation</strong></h5><p>Centrifugation of crude protein mixtures is an essential step in the investigation of protein purification and aggregation. Outlined below are the various types of centrifugations employed in protein purification.</p><p><strong>Preparative Ultracentrifugation</strong><br>Ultracentrifugation is an effective method of purifying proteins from aggregates based on their size, shape and density. Centrifugation, widely recognized as a robust and versatile method, is commonly used to separate complex mixtures of cellular components. Ultracentrifuges separate small particles by spinning at high speeds, utilizing centrifugal force based on size and density.</p><p><strong>Differential Centrifugation</strong><br>Differential centrifugation operates by separating components based on differences in size and density, using varying speeds of centrifugation to achieve this. Its resolving power ranges from low to medium. A key advantage of this technique is its convenience for processing multiple samples simultaneously, making it effective for purifying crude samples. Additionally, it preserves cell viability and maintains the natural distribution of hematopoietic cell populations.</p><p><strong>Rate-zonal Gradient Centrifugation</strong><br>This technique separates components based on their sedimentation rate within a medium that contains a density gradient. It offers medium resolving power and is particularly effective for separating multiple components in complex mixtures with high precision. It is also considered a cost-effective and widely used technique in molecular biology and biochemistry.</p><p><strong>Isopycnic Centrifugation (Equilibrium Centrifugation)</strong><br>Isopycnic centrifugation is an advanced protein purification method that relies on the principle of separating particles according to their intrinsic buoyant density, irrespective of their size or morphology. During the process, proteins migrate through a density gradient until they equilibrate at a position where their density matches that of the surrounding medium, resulting in precise separation without further sedimentation.</p></div></div></div><div><div><div><div><h4><strong>Chromatographic Approaches</strong></h4><p>Chromatography is a common method of protein purification and is based on the separation of molecules in a mixture as they move through a stationary phase and a mobile phase. Outlined below are the various types of chromatography employed in protein purification.</p><h5><strong>Ion Exchange Chromatography</strong></h5><p><a target="_blank" rel="noopener noreferrer" href="https://www.phenomenex.com/techniques/hplc-ion-exchange">Ion exchange chromatography</a> relies on the interaction between a charged stationary phase and oppositely charged analytes, with cation exchange chromatography attracting positively charged molecules to a negatively charged stationary phase. Successful application of this technique requires careful selection of the appropriate ion exchange column, buffer pH, and salt concentration. This approach is often used in the biopharmaceutical industries as mAb polishing steps, viral clearance and charge variant analyses as part of the product quality control.</p><p><a target="_blank" rel="noopener noreferrer" href="https://www.phenomenex.com/">Phenomenex</a> offers a range of protein purification columns. Their ion exchange chromatography solutions include:&nbsp;</p><ul><li><a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/products/1526"><strong>Luna SCX </strong></a>for strong selectivity of positively charged compounds, such as peptides</li><li><a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/products/1540"><strong>Biozen WCX</strong></a> for efficient separation of proteins such as monoclonal antibodies charged variants</li><li><a target="_blank" rel="noopener noreferrer" href="https://www.phenomenex.com/part/00g-3151-e0">PhenoSphere SAX</a>, a strong anion-exchanger with fully porous 80Å particles, particularly useful for small molecules such as nucleotides</li></ul><h5><strong>Size Exclusion Chromatography</strong></h5><p><a target="_blank" rel="noopener noreferrer" href="https://www.phenomenex.com/techniques/hplc-sec">Size exclusion chromatography (SEC)</a> separates molecules based on their size, with larger analytes eluting first due to their exclusion from the pores in the column packing material, while smaller molecules are able to enter the pores, facing a longer travelling path, and hence, eluting later. This method can be categorized into aqueous SEC (gel filtration chromatography, GFC) for analyzing biomolecules such as proteins and antibodies. This type of chromatography is widely used as an analytical tool for quality control in the biopharmaceutical industry and can also be an approach of choice for polishing steps.</p><p>Phenomenex offers a range of <strong>Biozen columns for SEC</strong> separations. In the case of mAbs, <a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/products/1534"><strong>Biozen dSEC-2</strong></a> and<a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/products/1537"><strong> Biozen dSEC-7 </strong></a>columns are the top choices for monoclonal antibody (mAb) analysis, delivering high-resolution separation of monomers, aggregates, and fragments formed during mAb manufacturing, processing, or storage. The dSEC-7 column, featuring a larger pore size and optimized particle design, extends the dynamic range to accommodate larger biomolecules and complex biotherapeutics.</p><p>For more general applications, <a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/products/1533"><strong>Biozen SEC-2</strong></a><strong> </strong>and <a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/products/1532"><strong>Biozen SEC-3</strong></a> phases cover different MW ranges for high-efficiency separation of biomolecules (e.g., proteins and antibodies). Additionally, the BioSep and Yarra lines become top choices when looking for preparative scale columns.</p><h5><strong>Reversed-Phase Chromatography</strong></h5><p><a target="_blank" rel="noopener noreferrer" href="https://www.phenomenex.com/techniques/hplc-reversed-phase">Reversed-phase chromatography</a>, the most common mode in high-performance liquid chromatography/ ultra-high-performance liquid chromatography (HPLC/UHPLC), separates compounds based on hydrophobic and van der Waals interactions using alkyl (C18, C8, C4) stationary phases or other hydrophobic polymers. For large biomolecules such as proteins and peptides, <a target="_blank" rel="noopener noreferrer" href="https://www.phenomenex.com/resources/blog/wider-pore-biozen-column">wide-pore</a> (typically 300 Å) C4, C8 and C18 columns are ideal, as the larger pore size allows better accessibility of the protein molecules to the stationary phase surface, improving mass transfer and peak shape during separation.</p><p>The Biozen line from Phenomenex offers a wide range of HPLC/UHPLC columns for the analysis of biomolecules.These include phases optimized for high-resolution analysis of intact proteins (Native-RP, Widepore C4, Intact XB-C8) and peptides (Peptide PS-C18, Peptide XB-C18 and Peptide Polar-C18). Kinetex could also be an option when looking for more selectivities for the analysis of peptides and small proteins. Additionally, when aiming to preparative scale processes, several of these media are also available in Phenomenex Axia formats to easily up-scale your separations.</p><h5><strong>Hydroxyapatite Chromatography</strong></h5><p>Hydroxyapatite chromatography (HAC) is a sophisticated mixed-mode chromatographic technique that exploits the unique properties of hydroxyapatite (Ca10(PO4)6(OH)2), utilizing both cation-exchange interactions via its phosphate groups and metal affinity through its calcium sites to achieve highly selective protein separations.</p><p>Renowned for its efficacy in resolving complex biomolecular separations, HAC excels in tasks like aggregate removal from monoclonal antibodies, surpassing the capabilities of traditional single-mode chromatographic methods such as ion-exchange or hydrophobic interaction chromatography.</p><h5><strong>Hydrophobic Interaction Chromatography</strong></h5><p>Hydrophobic interaction chromatography (HIC) is a method of protein purification that separates molecules based on their hydrophobicity, using high-salt buffers to promote protein-ligand interactions while maintaining the protein's biological activity. The process involves gradually reducing the salt concentration to elute proteins in order to increase hydrophobicity, making it effective for purifying target molecules and removing impurities or aggregates, making it a valuable approach for intermediate and polishing steps during purification processes.</p><h5><strong>Affinity Chromatography</strong></h5><p>Affinity chromatography is a powerful and highly selective technique used to purify biomolecules based on specific, reversible interactions between a target molecule and an immobilized ligand. In many cases, recombinant proteins are engineered with affinity tags—such as His-tags or GST-tags—that facilitate binding to complementary ligands on the chromatography matrix.</p><p>This method offers the advantage of achieving several thousand-fold purifications in a single step while preserving the biological activity of the protein. It is widely employed for purifying enzymes, antibodies, and antigens, using a variety of ligands including specific antibodies, enzymes, and multifunctional compounds like concanavalin A and triazine dyes.</p></div></div></div></div>

Exploring Protein Purification Methods: Essential Techniques for Accurate Results

<h3><span><strong>A Practical Guide to Getting the Most from Syringe Filters: Tips, Tricks, and Common Pitfalls</strong></span></h3><p><span><strong>Syringe filters play a crucial role in chromatography and many other laboratory applications by removing particulate matter before analysis. Proper sample filtration helps protect analytical instruments, extends column lifetime, and improves data quality. However, selecting an unsuitable filter—or using one incorrectly—can introduce contamination, reduce filtration efficiency, or even compromise analytical results. The following recommendations will help you avoid common mistakes and ensure reliable filtration every time.</strong></span></p><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/KNAUER_Avoiding_Common_Syringe_Filter_Mistakes_3_150e8c1c2b.webp" alt="KNAUER: Avoiding Common Syringe Filter Mistakes" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_KNAUER_Avoiding_Common_Syringe_Filter_Mistakes_3_150e8c1c2b.webp 133w," sizes="100vw" width="1276" height="1496"></p><h4><span><strong>1. Improper Filter Handling</strong></span></h4><p><span>Using excessive force during filtration or attempting to reuse disposable syringe filters can damage the membrane and increase the risk of sample contamination.</span></p><ul><li><span><strong>Solution:</strong> Apply slow, even pressure throughout the filtration process and always dispose of single-use filters after one use. If the flow rate decreases noticeably, replace the filter instead of applying additional force.</span></li></ul><h4><span><strong>2. Selecting an Inappropriate Pore Size</strong></span></h4><p><span>Using the wrong pore size may either fail to remove unwanted particles or cause the membrane to clog prematurely, reducing filtration efficiency.</span></p><ul><li><span><strong>Solution:</strong> A 0.45 µm membrane is generally suitable for routine particulate removal, while a 0.22 µm membrane is recommended for finer filtration and bacterial removal. Samples with higher viscosity may require a pre-filter to prevent rapid clogging. It is also important to consider the intended analytical technique. For example, 0.22 µm filters are typically preferred for UHPLC and LC-MS applications, whereas 0.45 µm filters are often sufficient for conventional HPLC analyses.</span></li></ul><h4><span><strong>3. Choosing the Wrong Membrane Material</strong></span></h4><p><span>Different membrane materials exhibit different chemical resistance. Using an incompatible membrane may lead to degradation of the filter, sample contamination, or complete filtration failure.</span></p><ul><li><span><strong>Solution:</strong> Always verify solvent compatibility before filtration. PTFE membranes are an excellent choice for organic solvents, while PES and nylon membranes are better suited to aqueous samples. If compatibility is uncertain, perform a small-scale trial before filtering valuable samples.</span></li><li><span><strong>Pro Tip:</strong> For additional guidance, see our blog article <strong>"How to Choose the Right Membrane for your Syringe Filter"</strong>, which provides a detailed comparison of membrane materials and their recommended applications.</span></li></ul><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/KNAUER_Avoiding_Common_Syringe_Filter_Mistakes_2_e47021fb11.jpg" alt="KNAUER Sepapure® Syringe Filters are available with multiple membrane materials, each identified by a dedicated color code for quick and reliable selection." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_KNAUER_Avoiding_Common_Syringe_Filter_Mistakes_2_e47021fb11.jpg 245w," sizes="100vw" width="1580" height="902"></p><h4><span><strong>4. Ignoring Filter Capacity</strong></span></h4><p><span>Using a filter with insufficient capacity can result in rapid membrane blockage, increasing filtration time and reducing laboratory productivity.</span></p><ul><li><span><strong>Solution:</strong> When filtering larger sample volumes, choose high-capacity syringe filters or introduce a pre-filtration step. Never force liquid through a clogged membrane, as doing so may damage the filter and negatively affect sample quality.</span></li></ul><h4><span><strong>5. Overlooking Sample Adsorption</strong></span></h4><p><span>Certain membrane materials may adsorb analytes of interest, leading to lower recoveries and potentially affecting quantitative results.</span></p><ul><li><span><strong>Solution:</strong> For protein samples or other adsorption-sensitive analytes, select membranes with low binding characteristics, such as nylon or glass fiber, whenever appropriate. Performing a small recovery test before routine analysis can help identify potential analyte losses.</span></li></ul><h4><span><strong>6. Failing to Optimize Filtration Performance</strong></span></h4><p><span>Slow filtration is more than just an inconvenience—it often indicates that the filtration conditions are not optimal.</span></p><ul><li><span><strong>Solution:</strong> If filtration becomes unusually slow, investigate possible causes such as membrane clogging, excessive sample viscosity, or an unnecessarily small pore size. Depending on the sample, dilution or sequential filtration using progressively finer filters may significantly improve performance.</span></li></ul><h4><span><strong>Final Thoughts</strong></span></h4><p><span>Selecting the appropriate syringe filter is a straightforward process, but overlooking small details can have a significant impact on analytical performance. Choosing the correct membrane material, pore size, and filtration strategy helps improve laboratory efficiency, protects chromatography systems and columns, and contributes to accurate, reproducible analytical results.</span></p><p><span>With the right syringe filter and proper filtration technique, you can minimize sample preparation problems and maximize confidence in every analysis.</span></p><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/KNAUER_Avoiding_Common_Syringe_Filter_Mistakes_4_983a15e289.jpg" alt="KNAUER: Sepapure® Syringe Filter Selection" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_KNAUER_Avoiding_Common_Syringe_Filter_Mistakes_4_983a15e289.jpg 245w," sizes="100vw" width="1160" height="627"></p><p><span><strong>Happy filtering!</strong></span></p>

Avoiding Common Syringe Filter Mistakes

News from LabRulezLCMS Library - Week 27, 2026

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Everything from the world of analytical chemistry in one place. We connect people in solving their problems. At Labrulez you will find all the necessary information easily, quickly and clearly. Stop searching and start finding.

LabRulez

Phenomenex is a global technology leader committed to developing novel analytical chemistry solutions that solve the separation and purification challenges of researchers in industrial, government and academic laboratories.

Phenomenex

Based in Berlin, KNAUER is a medium-sized, owner-managed company that has been serving the sciences since 1962. We develop and manufacture scientific instruments of superior quality for liquid chromatography, including: Analytical HPLC/UHPLC, Preparative HPLC, Fast protein liquid chromatography (FPLC), Multi-column chromatography/Simulated moving bed (SMB) chromatography. High pressure dosing, in-line detection systems, and osmometry are additional business areas.

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Agilent Technologies

Bruker

Thermo Fisher Scientific

Wiley

SCIEX

Waters Corporation

<p><strong>As oligonucleotide therapeutics rapidly emerge as a transformative class of medicines, ensuring their quality and safety has become more critical than ever. In this webinar, Xiaolei Zhuang from the United States Pharmacopeia (USP) will discuss challenges in oligonucleotide quality assessment and USP’s solutions to support reliable analysis. Dr. Bala Addepalli from Waters will explore how tunable enzymes and streamlined informatics enable precise sequencing of mRNA and associated impurities—all within a single LC-MS run.</strong></p><p><strong>Key Learning Objectives</strong></p><ul><li>Gain insights into overcoming analytical challenges in oligonucleotide therapeutics</li><li>Discover USP’s products and solutions that enhance quality and consistency</li><li>Explore comprehensive tools to streamline and automate oligonucleotide sequence confirmation, ensuring robust mapping workflows for routine RNA analysis in a GMP environment</li></ul><p><strong>Who Should Attend</strong></p><ul><li>Analytical scientists involved in oligonucleotide analysis</li></ul><blockquote><p><strong>Presenter: </strong>Xiaolei Zhuang (Principal Scientist, Global Biologics, US Pharmacopeia)</p><p>Xiaolei Zhuang is a Principal Scientist in Global Biologics at USP, where she leads the development of reference standards for mAb, insulin, endotoxin and other biologic products. She brings extensive expertise in analytical development, with a strong emphasis on bioassays. Prior to joining USP, she worked in the biopharmaceutical industry, supporting the development of biological therapeutics through analytical, preclinical, and clinical studies, with a focus on CMC components.</p><p><strong>Presenter:&nbsp;</strong>Dr Bala Addepalli, Ph.D., (Director R&amp;D, Waters Corporation)</p><p>Dr. Bala Addepalli has an impressive background in the field of cell and gene therapy product analysis. Currently, he is the Director of the Evaluations and Application Science Team under the Genetic Medicine analysis portfolio at Waters Corporation. His work focuses on developing efficient analytical approaches for characterization of DNA, RNA, LNPs(lipid nanoparticles), and viral vectors using liquid chromatography coupled with mass spectrometry or light scattering techniques. Before joining Waters, Bala made significant contributions at the University of Cincinnati, where he developed novel enzymes and analytical methods for RNA characterization. His earlier work includes research on mRNA polyadenylation at the University of Kentucky, and viral RNA characterization during his graduate studies in India. With over 60 publications and numerous presentations at scientific meetings, Bala's expertise is well-recognized in the scientific community.</p><p><strong>Moderated by:</strong> Samuel Seal (Biopharma Market Development Manager, Waters Corporation)</p><p>Samuel Seal is a market development manager specializing in the biologics space. He loves LC and MS – especially on how this technology provides benefit and value.</p><p>Sam joined Waters in January of 2014 as a Sales Technical Support Specialist in the Irvine office/lab. As a TSS he performed demonstrations on various instruments, provided training for customers, often presented on technical information at varying events, and assisted with troubleshooting from Colorado to Los Angeles. He previously worked at Pyxant Labs in Colorado Springs, CO where he gained extensive experience developing and validating methods for small/large molecules in bioanalytical assays, agricultural commodities, registration of pesticides, and worker exposure studies. He also has experience in column chromatography, sample extraction, sample clean-up, reporting and data management. Sam has hands on experience with running and maintaining various manufacturers' LC/MS/MS systems. Prior to Pyxant, Sam was a Laboratory Instructor at the University of Colorado at Colorado Springs where he earned his Bachelor of Arts in Biology and Bachelor of Arts in Chemistry.</p></blockquote>

Waters Corporation: Solving Problems That Matter - a Waters webinar series featuring USP speakers

Oligonucleotides

Explore oligonucleotide QC and mRNA sequencing using LC-MS. Hear from USP and Waters experts on challenges and analytical innovations.

<p><strong>Food safety laboratories face several challenges in meeting the demands of customers and regulators, often with the additional pressure of high sample numbers and the need for fast results. LC-MS/MS can address many of these challenges, but is often thought to be difficult to implement. SCIEX and Phenomenex are keen to ensure our users are supported and successful, so we have developed a number of workflows to address the most important applications. We hope these will allow both experienced scientists and those new to the technique to quickly implement methods and easily achieve the results they need.</strong></p><p>This online event is designed to introduce the concepts of LC-MS/MS for food safety analysis and to show how easy it can be to set up and optimise routine methods and to troubleshoot common issues.</p><p>The expert presenters will discuss all aspects of the workflow from sample preparation to reporting in compliance with regulations and will be on-hand to answer any questions throughout the session.</p><p>Please see the agenda below and register for access to this special course. Certificates of attendance can be provided on request and the presentations will be made available on-demand after the event.</p><h4><strong>Agenda</strong></h4><h5><strong>10:00 – 10:30: Welcome and introduction to SCIEX and Phenomenex</strong></h5><ul><li>Daniel McMillan, SCIEX</li></ul><h5><strong>10:30 – 11:00: Food sample preparation for LC-MS/MS analyses</strong></h5><ul><li>Nassima Amarouche, Phenomenex</li></ul><h5><strong>11:00 – 11:40: LC-MS/MS experimental design for food safety analyses</strong></h5><ul><li>Kévin Privat, SCIEX</li></ul><h5><strong>11:40 – 12:00: </strong>Q&amp;A / Break</h5><h5><strong>12:00 – 12:30: Column choice and chromatographic considerations</strong></h5><ul><li>Nassima Amarouche, Phenomenex</li></ul><h5><strong>12:30 – 13:10: Data processing &amp; reporting in accordance with food safety regulations</strong></h5><ul><li>Carlos Bueno, SCIEX</li></ul><h5><strong>13:10 – 13:40: Overview of latest SCIEX food safety applications</strong></h5><ul><li><strong>Daniel McMillan, SCIEX</strong></li></ul><h5><strong>13:40 – 14:00: </strong>Further Q&amp;A / End<br>&nbsp;</h5><blockquote><p><strong>Presenter:</strong> Kévin Privat (Application Support Specialist)</p><p>I obtained the master’s degree in analytical chemistry at the Paris-Saclay University in 2016. I am in Sciex for 7 years and I am focused on customer’s requests, trainings, and demos for the Southern Europe. I am currently focused on the TripleQuad and QTRAP systems, the Differential Mobility Separation and the EchoMS. I have also a good experience with Sciex OS software and especially with the calculated columns and flagging rules. Most of my time is spent analyzing in food or environmental matrices.</p><p><strong>Presenter:</strong> Nassima Amarouche (Business Development Manager, Phenomenex)</p><p>Nassima has a masters degree in Analytical Chemistry from Paris XI university, France and PhD in Separation Science from Reims University, France. Since 2013 at Phenomenex.</p><p><strong>Presenter:</strong> Carlos Bueno (Senior Application Support Specialist)</p><p>I finished my Bachelor Degree in Chemistry in 2001. Before starting working as applications specialist I worked 4 years in Madrid doping control laboratory. Working in Sciex since 2023 as Senior Application Support Specialist for EMEA. More than 20 years of experience in Chromatography and Mass spectrometry mostly focused on the development of application in food and environmental markets.</p><p><strong>Presenter:</strong> Daniel McMillan (Sr Market Development Manager, Food, Environmental &amp; Forensics (EMEAI), SCIEX)</p><p>Dan has been working in the MS industry for over 20 years as an instrument and applications specialist and is now responsible for developing the food, environmental and forensic markets throughout the EMEAI region. He has worked with major research and regulatory organisations to develop new applications in line with legislation, primarily in the area of contaminant analysis. Recent changes in the requirements imposed on and the challenges facing laboratories are constantly evolving, and Dan’s role is now primarily to ensure Sciex produce relevant solutions and to promote them in industry.</p></blockquote>

SCIEX: Spring school for food safety analysis

Spring school for food safety analysis

This online event is designed to introduce the concepts of LC-MS/MS for food safety analysis 

 Spring school for food safety analysis

<p><strong>Join François Espourteille on Tuesday, May 13, for our workshop "Rapid DART-MS Screen for Benzodiazepines in Urine Enabled by DART (Direct Analysis in Real Time) Technology" and discover the power of DART-MS in providing selective results, reducing false positives and negatives, and enabling rapid addition of new target analytes.</strong></p><p>Learn about our chromatography-free screening approach for benzodiazepines and their metabolites in urine, including alprazolam, clonazepam, diazepam, and more. This method measures 14 analytes in 96 samples with a throughput of 23 seconds per sample.</p><h5><strong>Learning Objectives:</strong></h5><ul><li>Understand the principles of DART-MS and its advantages over IA-based screening.</li><li>Learn the rapid, chromatography-free screening method for benzodiazepines.</li><li>Gain insights into sample preparation and analysis using EVOQ DART-TQ+ mass spectrometer.</li></ul><p>And don't miss the chance to hear <strong>Jeff Zonderman</strong> speak on the benefits and new developements of chromatogrphy-free workflows and join his talk “<strong>Bruker, Bringing Chrom-Free To The Masses</strong>” on Tuesday, May 13, at 10 am ET.</p><p>The workshop and talk are part of the 2025 Online Symposium on <strong>Current Trends in Forensic Toxicology</strong> by the CFSRE. The symposium, themed “<strong>Innovating for a Smarter, Sustainable, and Efficient Future in Forensic Toxicology</strong>,” will take place from May 12 - 16, 2025, with sessions from 8:45 am to 12 pm ET each day and afternoon workshops starting at 1 pm ET.</p><p>Enhance your knowledge of advanced screening techniques and improve accuracy and efficiency in measuring benzodiazepines. Register now to elevate your skills and stay ahead in the field of analytical chemistry!</p><blockquote><p><strong>Presenter: </strong>François Espourteille (Application Development Manager, Bruker Applied Mass Spectrometry)</p><p>François Espourteille brings over 25 years of expertise in LC-MSMS applications. He earned a BS and MS from The College of William and Mary in Virginia and received a PhD from Rutgers University, followed by a post-doctoral position at Rensselaer Polytechnic Institute. He joined Cohesive Technologies of Franklin, MA in the application group and subsequently managed the application group for Aria and Transcend platforms within Thermo Fisher Scientific until 2014.</p><p>For the following decade, François has focused on clinical/toxicological LC-MSMS assays, high-throughput workflows, and 2D LCMS platforms as a consultant. He joined Bruker Applied Mass Spectrometry (BAMS) in 2021 as the DART-MS and Triple Quadrupole Application Manager.</p><p><strong>Presenter:&nbsp;</strong>Jeff Zonderman (Senior Vice President of Bruker Applied Mass Spectrometry)</p><p>Jeffrey Zonderman serves as the Senior Vice President of Bruker Applied Mass Spectrometry, a new division of the company focused on providing mass spectrometry-based solutions for the applied market segments such as forensics, food, environmental, industrial/polymer, and clinical research. Jeffrey graduated from Northeastern University with a bachelor’s in biology and has been involved in mass spectrometry and chromatography for over 30 years. Before joining Bruker, Jeffrey worked in applications, sales/marketing and general management roles. His previous position was CEO of IonSense Inc, a company focusing on ambient ionization technology and commercialized DART (Direct Analysis in Real Time) technology. As Senior VP of Bruker Applied MS, Jeffrey looks to expand the applications of DART technology, amongst others, for chromatography-free mass spectrometry-based workflows.</p></blockquote>

Bruker: Rapid DART-MS Screen for Benzodiazepines in Urine Enabled by DART (Direct Analysis in Real Time) Technology

Rapid DART-MS Screen for Benzodiazepines in Urine Enabled by DART (Direct Analysis in Real Time) Technology

Discover power of DART-MS in providing selective results, reducing false positives and negatives, and enabling rapid addition of new target analytes.

Rapid DART-MS Screening of Benzodiazepines in Urine

<p><strong>Only one in four transplant waitlisted patients receive a life-saving organ. Xenotransplantation of pig organs into humans is a promising avenue for addressing organ shortage. Understanding the immune and physiological responses in these xenotransplants is crucial for future success in compassionate use and first-in-human trials. Seven gene-edited pig organs were transplanted into brain dead or living human in NYU in the last 3 years. These studies allowed us to generate and combine a number of omic datasets, including extensive transcriptomics spanning PBMC bulk RNA-seq, tissue and PBMC sc/snRNAseq and spatial transcriptomics; and various targeted and untargeted proteomic and metabolomic profiling. Plasma and urine samples were collected every day along with frequent xenograft tissue biopsies.&nbsp;</strong></p><p>This presentation focuses on blood proteomics and metabolomics in these xenotransplant recipients and illustrates the power of integrative omics to detect physiological and immunological changes in these unique experiments.<br><br><strong>Learning Objectives</strong></p><ul><li>Examine the potential of xenotransplantation as a solution to the organ shortage crisis, focusing on recent advancements and the transplantation of gene-edited pig organs into humans.</li><li>Understand the methodologies and significance of generating and integrating various omic datasets (transcriptomics, proteomics, and metabolomics) in studying immune and physiological responses in xenotransplant recipients.</li><li>Learn about the use of mass spectrometry to generate proteomics and metabolomics data.</li></ul><p>Webinars will be available for unlimited on-demand viewing after live event.</p><blockquote><p><strong>Presenter: </strong>Brendan Keating, PhD (Associate Professor, Division of Transplantation, Dept of Surgery and Institute of Systems Genetics, New York University Langone Health)</p><p>Brendan Keating, Is an Associate Professor in the Division of Transplantation, Department of Surgery, and The Institute of Systems Genetics, New York University (NYU) Langone Health, USA, with adjunct positions in University of Pennsylvania (UPenn) and the Children’s Hospital of Philadelphia. He received his D.Phil. (Ph.D.) in molecular genetics from Christ Church College, at the University of Oxford, UK with his thesis completed in the Department of Clinical Medicine, and the Wellcome Trust Center for Human Genetics. He completed a post-doctoral fellowship at the Institute of Translational Medicine and Therapeutics (ITMAT) and faculty position in Departments of Pediatrics and Surgery at UPenn, and he was also a visiting Scientist at the Wellcome Trust Sanger Institute, Cambridge, UK.</p><p>His Lab research interests focus on the analyses of polymorphisms and omics transplant donor and recipient’s genomes, to discover and validate genomic signals that underpin graft rejection and post-transplant complications in allotransplant, and pig to human xenotransplant settings. He instigated the formation of an international genomics consortium (iGeneTRAIN) for large-scale genomic studies. Dr. Keating also leads a Phase IV clinical trial for the early detection, diagnoses and treatment of influenza in transplant households using wearable devices.</p></blockquote>

Thermo Fisher Scientific: From pigs to patients: The omics edge in xenotransplantation

From pigs to patients: The omics edge in xenotransplantation

This presentation focuses on blood proteomics and metabolomics in these xenotransplant recipients and illustrates the power of integrative omics

<h4><strong>An introduction to accurate mass and Quadrupole-Time-of-Flight technology with SCIEX</strong></h4><p><strong>Until recently, nominal mass triple quadrupole technology was considered the gold standard for quantitative assays. In contrast, high-resolution mass spectrometry was primarily used for qualitative, semi-quantitative, and non-targeted workflows, such as metabolite identification, screening, and X-Omics. However, advancements in technology, including faster scan speeds and improved ion optics, have expanded the capabilities of high-resolution spectrometry. Now, it can be effectively applied to both qualitative and quantitative workflows.&nbsp;</strong></p><p>The ZenoTOF 7600 system, with its high scan rates and enhanced duty cycle at the MS2 level, bridges the gap between these approaches, offering a greater return on investment by reducing data acquisition times and maintaining sensitivity without compromising data quality. This webinar series will focus on how to transition from the Triple Quad to a high-resolution environment for different applications.</p><p>This webinar series will contain 3 parts. Each part will be 20 minutes with a 10 minute Q&amp;A.</p><h4><strong>Part 1: Fundamentals of accurate mass</strong></h4><ul><li>Theory behind accurate mass</li><li>How accurate mass works</li><li>Acquisition capabilities&nbsp;</li></ul><blockquote><p><strong>Presenter:</strong> Jessica Smith (Market Development Manager Software EMEAI, SCIEX)</p><p>Jessica joined SCIEX in 2021 as a technical marketing specialist; her responsibilities included managing projects throughout EMEAI and creating new technical marketing content. Her current role is Marketing Development Manager Software EMEAI, educating customers on the transition from Analyst software to SCIEX OS software. Jessica also studied for a Ph.D. in materials chemistry for environmental applications.</p></blockquote>

SCIEX: An introduction to accurate mass and Quadrupole-Time-of-Flight technology with SCIEX

Fundamentals of accurate mass

Join SCIEX specialists to learn about theory behind accurate mass and how accurate mass works.

<h4><strong>An introduction to accurate mass and Quadrupole-Time-of-Flight technology with SCIEX</strong></h4><p><strong>Until recently, nominal mass triple quadrupole technology was considered the gold standard for quantitative assays. In contrast, high-resolution mass spectrometry was primarily used for qualitative, semi-quantitative, and non-targeted workflows, such as metabolite identification, screening, and X-Omics. However, advancements in technology, including faster scan speeds and improved ion optics, have expanded the capabilities of high-resolution spectrometry. Now, it can be effectively applied to both qualitative and quantitative workflows.&nbsp;</strong></p><p>The ZenoTOF 7600 system, with its high scan rates and enhanced duty cycle at the MS2 level, bridges the gap between these approaches, offering a greater return on investment by reducing data acquisition times and maintaining sensitivity without compromising data quality. This webinar series will focus on how to transition from the Triple Quad to a high-resolution environment for different applications.</p><p>This webinar series will contain 3 parts. Each part will be 20 minutes with a 10 minute Q&amp;A.</p><h4><strong>Part 2: Advantages of HRMS for Quantitative Workflows</strong></h4><ul><li><strong>Efficiency and Cost Savings: </strong>Discover how HRMS quantitation can significantly reduce time and expenses</li><li><strong>Seamless Transition:</strong> Learn the steps to transition your established MRM assays to MRMHR with ease</li><li><strong>Streamlined Processes:</strong> Explore methods to streamline the entire workflow from biomarker discovery to quantitation&nbsp;</li></ul><blockquote><p><strong>Presenter:</strong> Heather Chassaing (Senior Accurate Mass Workflow Specialist, SCIEX)</p><p>Heather Chassaing has over 20 years of experience in analytical science, with a strong background in biochemistry. She has worked extensively in both the agrochemical and pharmaceutical industries, gaining comprehensive expertise in the research and development of drug entities, as well as the complexities of drug production. Heather is particularly passionate about structural elucidation and the application of Mass Spectrometry to these workflows.</p></blockquote>

Advantages of HRMS for Quantitative Workflows

Discover how HRMS quantitation can significantly reduce time and expenses and how to transition your established MRM assays to MRMHR with ease.

<div><div container-id="cq-colctrl-lt0" comp-id="A1"><div><p><strong>A recent study by the European Commission estimated that food fraud costs the global economy between €30 and €40 billion annually. This includes losses incurred by businesses due to product recalls, damage to brand reputation, and increased regulatory compliance costs.</strong></p><p><strong>Ongoing public demand for more information concerning food safety, authenticity, and origins continues to drive tighter regulations around the use, supply, and labelling of specific foods and ingredients – making this a primary concern for food businesses across many sectors. In many countries, food manufacturers must provide clear labelling to indicate when specific ingredients are present, including certain allergenic materials and genetically modified organisms (GMO), as well as to provide evidence of certain product claims, e.g. vegan.</strong></p><p>The average cost of a product recall can be around $10 million, and larger recalls can cost over $100 million. Overall, food fraud not only poses economic and ethical issues but also represents a serious public health risk, emphasizing the need for stringent food authenticity management and testing. Join our experts for a better understanding of food authenticity management for a better protection of the brands and the consumers.</p><h5><strong>Why Attend?</strong></h5><ul><li>Gain insights into the pressing challenges of food fraud and authenticity.</li><li>Learn from our experts about effective management strategies.</li><li>Discover the latest tools and technologies in food authenticity testing.</li><li>Engage with peers and participate in an interactive quiz</li></ul><h5><strong>Agenda:</strong></h5><ul><li><strong>What is Food Authenticity?</strong> Understanding the basics of food authenticity and its significance.</li><li><strong>Quiz: Let’s Play Together!</strong> An interactive quiz to test your knowledge on food authenticity testing and engage with the topic.<br>Impacts of Food Fraud: Why It Matters? Exploring the economic, health, and reputational impacts of food fraud on the global food industry.</li><li><strong>How to Implement Food Authenticity Management Case study</strong> and practical strategies and management practices to ensure food authenticity within your organization.</li><li><strong>Available Testing Tools and Benefits:</strong> A look at the latest testing tools available to combat food fraud and their benefits.</li><li><strong>Closing remarks and Q&amp;A</strong></li></ul><blockquote><p><strong>Presenter: </strong>Sandra Fréville (Tactical Marketing Manager EM/APJ Food Industry, Thermo Fisher Scientific MBD)</p><p>I'm the Regional Tactical Manager in the Microbiology division of Thermo Scientific dedicated to Industries. I'm graduated in biochemistry technologies and obtained a master’s degree in sale technics applied to biotechnology devices and reagents at the Superior School of Technics applied to Biology (ESTBA) in Paris. Bring and highlight solutions that help food industry to face its daily challenges and that guarantee safe products from the farm to the fork makes really sense to me. Being useful to the food industry actors by contributing to food safety culture improvement drove me to evolve for the last 20 years in sales and marketing applied to food diagnostic with an area of focus in alternative validated cultural and molecular methods for food pathogen testing.</p><p><strong>Presenter: </strong>Irene Rapti (Senior Product Manager- Molecular Solutions, Thermo Fisher Scientific)</p><p>I am a global health technology expert with 25 years of experience driving commercial excellence in the healthcare industry. Skilled in product management across both small enterprises and large corporations, I have a proven track record of managing highly regulated and innovative products, including molecular diagnostic IVD tests. My expertise encompasses Upstream and Downstream Marketing, Strategic Marketing Plans, Product Roadmaps, Product Launches, PPI, and New Product Development. Having lived and worked in the UK and Greece, I currently collaborate remotely with teams in over 20 countries, covering key regions such as Europe, the US, and Asia Pacific. I excel at motivating and engaging international teams to anticipate and respond to market trends. My educational background includes a degree in Biotechnology from London South Bank University and a Master's in Molecular Medicine from UCL.</p></blockquote></div></div></div>

Thermo Fisher Scientific: Food Integrity: Strategies to Ensure Authenticity and Combat Fraud

Food Integrity: Strategies to Ensure Authenticity and Combat Fraud

Gain insights into the pressing challenges of food fraud and authenticity 

Food Integrity: Ensuring Authenticity and Preventing Fraud

<h4><strong>Overview</strong></h4><p>In this webinar, we will address the main LC-MS/MS solutions for the determination of short chain PFAS compounds alone or in combination with the analysis of medium- to long-chain PFAS. The webinar is aimed at everyone involved in the development of analytical methods for the analysis of PFAS, Ultra Short Chain PFAS, and TFA, with the goal of minimizing instrumental contamination from PFAS.&nbsp;</p><h4><strong>Key Learning Points</strong></h4><ul><li>Walk through a PFAS chemistry overview</li><li>Review Short Chain PFAS and TFA: Contamination Management</li><li>Understand emerging analytical solutions</li><li>Learn how to combine Short chain PFAS and “conventional” PFAS</li></ul><h4><strong>Who Should Attend</strong></h4><ul><li>All scientists and researchers, interested in or currently conducting PFAS analysis</li></ul><blockquote><p><strong>Speaker: </strong>Sam Lodge (Senior Business Development Manager, Environmental,<strong> </strong>Phenomenex)</p><p>Sam Lodge is the Phenomenex Senior Business Development Manager for the Environmental industry. He has been at Phenomenex for over 30 years in various positions, including Key Account Manager and Senior International Sales Manager, and is a product expert in SPE and HPLC chemistries. Over the past 8 years he has worked extensively with commercial and government labs in the development and support of PFAS analytical methods, including the validation of EPA 533, the MLV for DM 1633, and many internal (MOD) methods. Sam was instrumental in the collaborations that led to the development of several new SPE formats for PFAS, including several different Stacked Tubes and Pass Through GCB cartridges that can help commercial labs to increase productivity and reduce costs. In his spare time Sam enjoys fishing and surfing (but not at the same time!)</p><p><strong>Speaker: </strong>Luigi Margarucci, PhD (EMEA Seminar and Application Lead, Phenomenex)</p><p>Luigi holds a PhD in Pharmaceutical Sciences and began his career at Phenomenex in 2015. He gained experience in the field of chromatography coupled with mass spectrometry while working as a researcher/fellow at the University of Salerno and collaborating with major reference centers for chromatography and mass spectrometry in Italy and Europe (Utrecht University - NL). To date, he is the author of 27 scientific publications and has written and presented a huge number of seminars on analytical techniques such as LC (LCMS), GC, and SPE.</p></blockquote>

Phenomenex: Alternative Method Parameters for PFAS Short Chain Detection

Alternative Method Parameters for PFAS Short Chain Detection

In this webinar, we will address the main LC-MS/MS solutions for the determination of short chain PFAS compounds

Alternative Methods for PFAS Short Chain Detection

<div><p><strong>Sequence variants in protein therapeutics can significantly impact the biological activity of a drug. These mutations may alter protein structure, leading to undesirable immune responses in patients, compromising drug stability or reducing therapeutic efficacy. Detecting and controlling sequence variants is a critical regulatory requirement and early detection allows for necessary adjustments in the manufacturing process to ensure batch consistency.</strong></p><p>This webinar will explore how to detect challenging sequence variants by applying an icIEF-UV/MS workflow on the Intabio ZT system. Additionally, we will discuss the subsequent characterization using EAD-enabled LC/MS methods on the ZenoTOF 7600 system. We will also explore a case study that highlights how a cell and developability science team identified a previously uncharacterized sequence variant in a CMC program, and utilized novel workflows to develop a mitigation strategy for monitoring this variant in current and future programs.</p><h5><strong>Key Learnings:</strong></h5><ul><li><strong>Impact of variants </strong>- Learn how sequence variants affect drug efficacy, stability and immune responses</li><li><strong>Detection methods </strong>- &nbsp;Discover icIEF-UV/MS and EAD-enabled LC/MS techniques for identifying variants</li><li><strong>Case study</strong> - Gain insights from a real-world example of identifying and mitigating a sequence variant</li></ul><h5><strong>Who should attend:</strong></h5><ul><li>Principal Scientist</li><li>Senior Scientist</li><li>Analytical Development</li><li>Cell Line Development</li><li>Associate Director<br>&nbsp;</li></ul><blockquote><p><strong>Presenter:</strong> Kristen Nields (Principal Scientist, Johnson &amp; Johnson Innovative Medicine)</p><p><strong>Presenter: </strong>Riley Schaefer (Co-Op Scientist II, Johnson &amp; Johnson Innovative Medicine)</p></blockquote></div>

Wiley: From Detection to Mitigation: Managing Sequence Variants in Biotherapeutics

From Detection to Mitigation: Managing Sequence Variants in Biotherapeutics

This webinar will explore how to detect challenging sequence variants by applying icIEF-UV/MS workflow on the Intabio ZT system

Managing Sequence Variants in Biotherapeutics

<p><strong>Synthetic peptides have emerged as a significant class of therapeutic agents, particularly in the management of metabolic disorders such as type 2 diabetes mellitus. Among these, glucagon-like peptide-1 (GLP-1) receptor agonists have gained prominence due to their multifaceted role in glucose regulation and weight management. Over the past decade, we have witnessed a rise in approvals for these molecules, and the generics market is gearing up as some of these drugs approach patent expiration.</strong></p><p>Due to the diverse amino acid sequences and structural complexities, synthetic peptides require robust and precise analytical methods to ensure their quality, efficacy, and safety. However, the lack of harmonized regulatory guidelines for peptide characterization presents significant challenges. Certain critical quality attributes (CQAs) are widely accepted among the peptide community, necessitating sophisticated analytical techniques.</p><p>Given the ongoing speculation in the market regarding the best analytical techniques for synthetic peptides, we are hosting this event to provide a platform for analytical users and chemists working with peptides to ask their queries directly to experts like yourself.</p><p>Don’t let workflow challenges slow you down. Join us to unlock the full potential of your workflows, enhance your technical skills, and take a significant step in your professional development.</p><blockquote><p><strong>Presenter:</strong> Dr. Vasanthakumar G. Ramu (Vice President – Head R&amp;D, Peptides and Complex API’s, Alembic Pharmaceutical Ltd.)</p><p>Dr. Vasanthakumar Ramu, is one of the pragmatic scientists in the pharma industry working around complex API’s. He studied chemical biology from Bangalore University and Indian Institute of Science. In early 2007, hemoved to Max-Planck Institute of Molecular Physiology to work on peptide decorated supramolecular architectures as modulators to study cell protein interactions. Continued further studies as research scientist in Ruhr Universität Bochum, Germany, with the objective to work on backbone modified peptides of HIV protease inhibitors. In 2011 spring, moved to Spain, where he was working as Marie Curie Experienced Researcher to develop Kyotorphin derived peptides, antimicrobial peptides, and other small molecules for neurodegeneration. Returned to India in 2012 and led the startup company called Omgene Life Sciences Pvt. Ltd. Before joining Alembic, was heading peptide process development groups in Biocon Ltd., and Aurobindo Pharma Ltd., Transferred the technologies, successfully validated and filed various DMF’s and involved in commercialization of API’s.</p><p><strong>Presenter:</strong> Dr. Rakesh Shekhawat (Founder/Owner, Proantek Research Labs Pvt. Ltd)</p><p>Dr. Rakesh Shekhawat is a distinguished scientist with over 20 years of industrial research experience in the fields of synthetic peptides, biosimilars, and oligonucleotide characterization. Holding a Ph.D. in analytical chemistry, Dr. Shekhawat has led analytical research teams at prominent companies such as Intas and USV, contributed significantly to securing various regulatory approvals for biosimilars and synthetic peptides. Recently, he founded Proantek Research Labs Pvt. Ltd., a company dedicated to the advanced characterization of biosimilars, synthetic peptides, and oligonucleotides.</p><p><strong>Presenter:</strong> Dr. Vadi Bhat (Sales Development Manager - Biopharma, Agilent Technologies)</p><p>Vadi Bhat, Ph.D., is a seasoned biopharma business development manager at Agilent Technologies in India, bringing extensive expertise in biopharmaceutical research and development. He holds a Ph.D. in chemical biology from JNCASR/IISc and completed postdoctoral research at MIT. Dr. Bhat's career includes experience in mass spectrometry core facility management at Baylor Scott &amp; White Hospital/Texas A&amp;M University.</p><p>Since joining Agilent in 2008, he has played a key role in developing innovative LC-MS workflows for proteomics and biopharma applications, initially at Agilent's Little Falls Center of Excellence in Delaware. From 2015 to 2017, he served as a proteomics market specialist within Agilent's North America Field Team, driving customer collaborations and business development. Transitioning to his current role in India in 2017, he focuses on fostering collaborations and driving business growth in the biopharma sector. Dr. Bhat's contributions to the field are reflected in numerous peer-reviewed publications and presentations at scientific conferences. In 2023, he was appointed adjunct professor at the Manipal Centre for Biotherapeutics Research (MCBR), MAHE, further demonstrating his dedication to advancing biopharma research.</p><p><strong>Presenter:</strong> Dr. Ashish Pargaonkar (Biopharma Workflow Commercialization Lead, Agilent Technologies)</p><p>Dr. Ashish Pargaonkar is a Lead for Biopharma Workflow Commercialization team for India and also Manager for Centre of Excellences at Bangalore. A Microbiologist by education with a Ph.D, he holds more than 20 years of experience for Biopharma market applications on Hi Resolution Mass Spectrometry. He has a long record of successful demonstrations and customer engagements on workflows for CQA’s, Intact mass, peptide mapping, HCP and Glycans using Agilent state-of-the art solutions. He has authored ASMS posters on media components and therapeutic peptides. Dr. Ashish has been invited speaker at several conferences and workshops at Biosimilar Congress, ADC 2018, IIT (Mumbai, Delhi), ICT (Mumbai), PSI events.</p><p><strong>Presenter:</strong> Dr. Sunil B Lakhmapure (Technical Support Specialist, Agilent Technologies India Pvt. Ltd.)</p><p>Sunil holds 19 year plus experience in the analytical field with major exposure to LC/GC with Single Quad, Triple Quad and high resolution (QToF) mass spectroscopy. Started career in Pharma R&amp;D as a scientist with hands on experience on high end instruments like LC/MS, XRD &amp; NMR. Developed &amp; published application notes using Chromatographic techniques on real world samples/issues at various levels.</p></blockquote>

Agilent Technologies: Ask The Expert - Peptide Workflow

Ask The Expert - Peptide Workflow

Join us to unlock full potential of your workflows, enhance your technical skills

LCMS webinars focusing on LC/HRMS - page 15

Advantages of HRMS for Quantitative Workflows

Ask The Expert - Peptide Workflow

Oligonucleotides

Alternative Method Parameters for PFAS Short Chain Detection

Rapid DART-MS Screen for Benzodiazepines in Urine Enabled by DART (Direct Analysis in Real Time) Technology

Fundamentals of accurate mass

Food Integrity: Strategies to Ensure Authenticity and Combat Fraud

From Detection to Mitigation: Managing Sequence Variants in Biotherapeutics

From pigs to patients: The omics edge in xenotransplantation

Spring school for food safety analysis

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