Scientists at IOCB Prague are uncovering new details of gene transcription. They have identified a previously unknown molecular mechanism by which the transcription of genetic information from deoxyribonucleic acid (DNA) into ribonucleic acid (RNA) can be initiated. The researchers focused on a specific class of molecules known as alarmones, which are found in cells across a wide range of organisms and whose levels often increase under conditions of cellular stress. The results were published in the prestigious scientific journal Nature Chemical Biology.<img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/UOCHB_Vyzkum_z_UOCHB_odhaluje_dosud_nepoznany_zpusob_prepisu_geneticke_informace_bc2bfffa6c.jpg" alt="IOCB Prague/Tomáš Belloň: Research from IOCB Prague reveals a previously unknown mechanism of genetic transcription: Dr. Hana Cahová, head of the Chemical Biology of Nucleic Acids research group." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_UOCHB_Vyzkum_z_UOCHB_odhaluje_dosud_nepoznany_zpusob_prepisu_geneticke_informace_bc2bfffa6c.jpg 234w," sizes="100vw" width="2000" height="1335"><div>Molecules of ribonucleic acid can carry a variety of chemical modifications at one end, known as caps. In eukaryotic organisms, including human cells, the best-known cap plays an important role in RNA stability and in regulating its subsequent fate. In recent years, however, it has become clear that alternative, non-canonical RNA caps also exist, although their formation and the mechanisms by which they are attached to RNA remain only partially understood. These include alarmone caps, formed by dinucleoside polyphosphate molecules, that protect cellular RNA at moments when the cell is under threat.In their study, <a target="_blank" rel="noopener noreferrer" href="https://cahova.group.uochb.cz/en">Dr. Hana Cahová</a> and colleagues examined bacterial RNA polymerase and investigated how this enzyme can initiate transcription using dinucleoside polyphosphates (NpNs) instead of the standard RNA building blocks. For the first time, the scientists described, at the atomic level, how RNA bearing an alarmone cap can be generated directly at the initiation of gene transcription. They also observed that NpNs bind through a different type of base pairing than is typically observed.The work was significantly shaped by Valentina Serianni from Hana Cahová’s team, who demonstrated the ability of dinucleoside polyphosphates to initiate gene transcription, and by Jana Škerlová, who focused on the structural analysis of RNA polymerase. Using cryogenic electron microscopy data, she showed how dinucleoside polyphosphate molecules bind within the active site of RNA polymerase – that is, the core of the enzyme where genetic information is transcribed. Together, their findings help to elucidate the processes that accompany the transcription of genetic information from DNA to RNA.Hana Cahová adds: “We’re describing something that truly occurs in cells and that we’re now able to observe directly at the level of individual molecules. This allows us to answer fundamental questions about cellular processes, such as how cells adapt to stress. RNA plays a central role in this, as it carries the cascade of information underlying any cellular response – for example, to threatening conditions caused by nutrient deprivation or temperature shock.”<img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/UOCHB_Vyzkum_z_UOCHB_odhaluje_dosud_nepoznany_zpusob_prepisu_geneticke_informace_2_0fe88ff9e9.jpg" alt="IOCB Prague/Tomáš Belloň: Research from IOCB Prague reveals a previously unknown mechanism of genetic transcription: Dr. Tomáš Kouba, head of the Cryogenic Electron Microscopy core facility." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_UOCHB_Vyzkum_z_UOCHB_odhaluje_dosud_nepoznany_zpusob_prepisu_geneticke_informace_2_0fe88ff9e9.jpg 234w," sizes="100vw" width="2000" height="1335"><div>Cryogenic electron microscopy (cryo-EM) was central to the project. This part of the work was led by Dr. Tomáš Kouba, one of the authors of the study. “Cryogenic electron microscopy allows us to freeze biological molecules in a state very close to their natural form and then determine their three-dimensional structure. This makes it possible to look directly into the active centers of enzymes and observe their function down to the atomic level,” he explains.Last year, IOCB Prague opened a new <a target="_blank" rel="noopener noreferrer" href="https://cryoem.group.uochb.cz/en">cryo-EM center</a> that is unique within the Czech research environment. State-of-the-art cryogenic electron microscopes are housed in a specially designed building and enable the study of biological processes with exceptional structural precision.<img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/UOCHB_Vyzkum_z_UOCHB_odhaluje_dosud_nepoznany_zpusob_prepisu_geneticke_informace_3_4d881e7a58.jpg" alt="IOCB Prague: Research from IOCB Prague reveals a previously unknown mechanism of genetic transcription: This work shows how NpnNs initiate bacterial transcription. Cryo-EM reveals how nucleobases pair with the template: one in canonical and one in reverse Watson–Crick mode, providing a structural basis for their function as non-canonical RNA caps." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_UOCHB_Vyzkum_z_UOCHB_odhaluje_dosud_nepoznany_zpusob_prepisu_geneticke_informace_3_4d881e7a58.jpg 245w," sizes="100vw" width="2000" height="1077"><blockquote><h4>The Original article</h4><h4><a target="_blank" rel="noopener noreferrer" href="https://www.nature.com/articles/s41589-025-02134-5">Molecular insight into 5′ RNA capping with NpnNs by bacterial RNA polymerase</a></h4>Valentina M. Serianni, Jana Škerlová, Anna Knopp Dubánková, Anton Škríba, Hana Šváchová, Tereza Vučková, Anatolij Filimoněnko, Milan Fábry, Pavlína Řezáčová, Tomáš Kouba &amp; Hana Cahova&nbsp;Nat. Chem. Biol. 21 (2026)<a target="_blank" rel="noopener noreferrer" href="https://doi.org/10.1038/s41589-025-02134-5">https://doi.org/10.1038/s41589-025-02134-5</a><h6>licensed under <a target="_blank" rel="noopener noreferrer" href="https://creativecommons.org/licenses/by/4.0/">CC-BY 4.0</a></h6></blockquote><h4>Abstract</h4>RNA capped with dinucleoside polyphosphates has been discovered in bacteria and eukaryotes only recently. The likely mechanism of this specific capping involves direct incorporation of dinucleoside polyphosphates by RNA polymerase as noncanonical initiating nucleotides. However, how these compounds bind into the active site of RNA polymerase during transcription initiation is unknown. Here, we explored transcription initiation in vitro, using a series of DNA templates in combination with dinucleoside polyphosphates and model RNA polymerase from Thermus thermophilus. We observed that the transcription start site can vary on the basis of the compatibility of the specific template and dinucleoside polyphosphate. Cryo-electron microscopy structures of transcription initiation complexes with dinucleoside polyphosphates revealed that both nucleobase moieties can pair with the DNA template. The first encoded nucleotide pairs in a canonical Watson–Crick manner, whereas the second nucleobase pairs noncanonically in a reverse Watson–Crick manner. Our work provides a structural explanation of how dinucleoside polyphosphates initiate RNA transcription.</div></div>

Research from IOCB Prague reveals a previously unknown mechanism of genetic transcription

Food adulteration is a phenomenon with a long history that has gained increasing importance in recent years due to the globalization of food supply chains and strong economic pressures. Whether it involves diluting olive oil with cheaper oils, adding sugar to honey, or the hidden use of substitutes in protein supplements, the result is a loss of consumer trust and, in some cases, a potential risk to health. Analytical chemistry plays a key role in this area by providing methods and technologies capable of identifying and quantifying target compounds as well as unwanted impurities in a wide range of food matrices.<img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Watrex_Praha_Falsovani_potravin_a_jak_ho_odhali_analyticka_chemie_6912db5492.jpg" alt="Watrex: Food Adulteration and How It Is Revealed by Analytical Chemistry" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Watrex_Praha_Falsovani_potravin_a_jak_ho_odhali_analyticka_chemie_6912db5492.jpg 245w," sizes="100vw" width="1600" height="900"><h4>The Role of Chromatography in Forensic Food Analysis</h4>Chromatography, particularly liquid chromatography (HPLC), is one of the most effective analytical tools for separating complex mixtures of molecules. It enables:<ul><li>qualitative identification of individual components in a sample,</li><li>quantitative determination of their concentrations,</li><li>detection of impurities and unexpected components that may indicate adulteration.</li></ul>In practice, HPLC is often combined with various types of detectors (UV/VIS, DAD, refractive index detectors) or with other techniques (e.g., mass spectrometry) to increase selectivity and sensitivity.<h4>Isocratic vs. Gradient Elution</h4>In the analysis of food samples, chromatographic methods are selected according to the analytical goal:<ul><li>Isocratic HPLC uses a constant mobile phase composition throughout the analysis. It is simple, robust, and ideal for routine determinations of well-defined target analytes (e.g., sugars or vitamins).</li><li>Gradient HPLC changes the mobile phase composition during separation, enabling better resolution of complex mixtures with a wide range of polarities (e.g., peptides or phenolic compounds).</li></ul>Gradient methods are particularly useful when analytes have very different properties and high separation efficiency is required.<h4>Watrex Praha and the Contribution of Modern Systems to Food Analysis</h4><a target="_blank" rel="noopener noreferrer" href="https://watrex.com/?utm_source=labrulez&amp;utm_medium=propagace&amp;utm_campaign=labrulez_clanky_HPLCsystemy">Watrex Praha, s.r.o.</a> is one of the leading Czech manufacturers and distributors of liquid chromatography systems and instruments, with a long tradition dating back to 1990. The company has successfully transformed from a distributor into a manufacturer of its own HPLC and related technologies, focusing on modular, robust systems suitable even for demanding analytical tasks.Their <a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/products?manufacturerItems=93&amp;search=streamline">Streamline™ product line</a> includes several HPLC system concepts suitable for food analysis:<ul><li>Isocratic HPLC system – a simple yet precise configuration with a single pump, column thermostat, and UV/VIS detector; suitable for routine determination of known analytes (e.g., basic carbohydrates, food additives).</li><li>Binary gradient HPLC system – equipped with two pumps enabling mobile phase gradient formation, which enhances separation performance for complex mixtures (e.g., phenolic profiles of wine, components of aromatic extracts).</li><li>GPC/SEC system (Gel Permeation / Size Exclusion Chromatography) – a specialized solution for the determination of macromolecules and polymers, also relevant for the analysis of high-molecular-weight components in foods (e.g., polysaccharides).</li></ul><blockquote>👉<a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/labrulez-bucket-strapi-h3hsga3/Watrex_catalog_2024_ba97395f92.pdf">Product catalogue HERE</a></blockquote>Thanks to the modular nature of these systems, analysts can easily adapt the configuration to a specific analytical problem, from simple routine analyses to complex characterization of food samples.<h4>Practical Examples of Adulteration Revealed by HPLC</h4><ul><li>Detection of olive oil dilution: HPLC can separate and quantify characteristic triacylglycerols and fatty acids; unusual patterns indicate the presence of cheaper oils.</li><li>Estimation of sugar addition in honey: Isocratic HPLC with a refractive index detector can determine sugar profiles (glucose, fructose, sucrose), revealing dilution or the addition of sugar syrups.</li><li>Identification of aromatic adulterants in wine: Gradient HPLC with a PDA detector can reveal phenolic and aromatic profiles that are altered by the addition of synthetic compounds.</li></ul><h4>Conclusion</h4>Food adulteration remains a current issue that requires reliable and sensitive analytical methods. Liquid chromatography, and in particular modern HPLC systems, represent one of the most powerful tools for detecting unwanted additives, impurities, and unexpected components in complex food samples. Manufacturers such as <a target="_blank" rel="noopener noreferrer" href="https://watrex.com/?utm_source=labrulez&amp;utm_medium=propagace&amp;utm_campaign=labrulez_clanky_HPLCsystemy">Watrex Praha</a> provide flexible chromatographic platforms covering a wide range of analytical tasks from simple isocratic to advanced gradient and GPC/SEC applications thereby supporting analysts in ensuring food quality and consumer protection.<blockquote>Did you find this article interesting? Follow <a target="_blank" rel="noopener noreferrer" href="https://watrex.com/?utm_source=labrulez&amp;utm_medium=propagace&amp;utm_campaign=labrulez_clanky_HPLCsystemy">Watrex Praha</a> on their social media (<a target="_blank" rel="noopener noreferrer" href="https://www.linkedin.com/company/watrex/?viewAsMember=true">LinkedIn</a> and <a target="_blank" rel="noopener noreferrer" href="https://www.facebook.com/WatrexPrague">Facebook</a>) for more engaging topics.</blockquote>

Food Adulteration and How It Is Revealed by Analytical Chemistry

Join us for a fascinating conversation with Dr. Subhoja Chakraborty from the University of Central Florida, whose cutting-edge research targets the global challenge of malaria drug resistance. Dr. Chakraborty shares her journey from structure-based drug design during her PhD to her recent collaborative work repurposing human kinase inhibitors as rapid, selective antimalarials against drug-resistant Plasmodium falciparum strains.<h4>In this episode, you'll learn:</h4><ul><li>How structure-guided approaches reveal potential drug targets, including cysteine proteases like falcipain-2, and kinases implicated in malaria's complex life cycle.</li><li>The power of repurposing kinase inhibitors originally developed for cancer and other diseases, focusing on compound 12, which blocks hemozoin formation and protein kinase 6—key steps in parasite survival.</li><li>Lab techniques such as affinity, gel filtration, and size exclusion chromatography for recombinant protein purification—and how troubleshooting is crucial for high-yield, stable complexes.</li><li>Advances in mass spectrometry for characterizing protein interactions, validating drug targets, and mapping molecular pathways pivotal to malaria therapeutics.</li><li>Tips and lessons for new researchers facing the challenges of sample preparation, complex protocols, and working as part of a collaborative team.</li></ul>Dr. Chakraborty’s insights bridge the gap between basic science and translational drug discovery, offering hope for more effective, targeted malaria treatments in a world where resistance to frontline therapies threatens millions.<h4>Referenced Papers:</h4><ul><li>"Plasmodium falciparum protein kinase 6 and hemozoin formation are inhibited by a type II human kinase inhibitor exhibiting antimalarial activity" (Cell Chemical Biology, 2025)</li><li>"New insights of falcipain 2 structure from Plasmodium falciparum 3D7 strain" (BBRC, 2022)</li><li>"Structure-Based Optimization of Protease−Inhibitor Interactions to Enhance Specificity of Human Stefin‑A against Falcipain‑2 from the Plasmodium falciparum 3D7 Strain" (Biochemistry, 2023)</li></ul>If you’re a student, scientist, or simply passionate about advances in infectious disease research, this episode offers actionable insights, inspiring advice, and a front-row seat to innovation in combating malaria.<h3>Video Transcription</h3><h4>From Structural Biology to Drug Discovery: Chromatography and Mass Spectrometry in Malaria Research</h4><h5>Introduction</h5>In this discussion, Subhoja shares insights into her research journey, focusing on malaria drug discovery and the role of modern analytical techniques in biological and biomedical research. The conversation is aimed primarily at undergraduate students and early-career researchers interested in chemistry, biotechnology, and life sciences.<h5>Research Focus: Malaria Drug Discovery and Kinase Targets</h5>Subhoja is currently involved in malaria drug discovery research at the University of Central Florida, where she has been working since beginning her postdoctoral position two years ago. Her earlier PhD research focused on structure-based drug design in malaria, using a combination of in silico approaches, recombinant protein expression, and X-ray crystallography to determine precise three-dimensional structures of target proteins and their inhibitors.This structural perspective provided a foundation for her current work, which centers on drug discovery through compound screening and repurposing. A recent publication from her laboratory explores the use of repurposed mammalian CDK-like kinase inhibitors as potential antimalarial agents. While such kinase inhibitors are well known in cancer and cardiovascular research, their application to malaria targets represents a novel and promising approach.Kinases are attractive drug targets because of their central role in cell signaling and growth pathways. By targeting specific kinases involved in different stages of the malaria parasite life cycle—whether asexual growth, gametocytogenesis, or differentiation—it may be possible to significantly inhibit parasite growth and progression.<h5>Chromatography in Protein Expression and Purification Workflows</h5>Chromatography plays a critical role throughout Subhoja’s research. Her experience spans from basic chromatography techniques during her early training to advanced protein purification workflows using affinity chromatography, gel filtration (size exclusion chromatography), and ion exchange chromatography.Recombinant proteins are routinely purified using affinity resins such as nickel or cobalt, followed by further purification to remove contaminants. Fast Protein Liquid Chromatography (FPLC) enables compact, stepwise purification workflows suitable for producing proteins for in vitro assays.A recurring challenge in this process is the expression of high-molecular-weight proteins, which often accumulate in insoluble fractions or inclusion bodies. Careful selection of expression vectors, host systems, and induction conditions is therefore essential to maximize yield and solubility.<h5>Integrating Size Exclusion Chromatography into the Workflow</h5>Size exclusion chromatography (SEC) is typically used after affinity purification to further improve protein purity. Following elution with imidazole or glutathione, desalting is required to prevent buffer components from interfering with downstream assays. This is often achieved using desalting columns such as G-25 or PD-10.To remove lower-molecular-weight contaminants, finer SEC media—such as Superdex or Sephacryl columns—are employed. Concentration steps using molecular-weight cut-off filters are then applied to obtain the desired protein concentration for functional studies.These iterative purification and troubleshooting steps are a routine part of protein biochemistry and reflect the importance of methodical optimization and careful validation.<h5>The Role of Mass Spectrometry in Biomolecular Characterization</h5>Mass spectrometry is a key analytical tool in Subhoja’s research pipeline, particularly for target identification and validation. In both malaria and cancer research, MS is used alongside chromatography, immunoprecipitation, and pull-down assays to study protein interactions and molecular recognition events.Affinity-based mass spectrometry enables the identification of binding partners and interactors, whether studying proteins, long non-coding RNAs, or protein complexes. Techniques such as native MS, tandem affinity purification, and proximity labeling (e.g., TurboID-based workflows) provide deeper insights into molecular networks and signaling pathways.These approaches are essential for understanding how candidate drug targets function within complex biological systems.<h5>Sample Preparation Challenges in Biological Research</h5>Sample preparation remains one of the most challenging aspects of biological experimentation. Whole-cell lysates contain proteases, nucleases, and other components that can rapidly degrade target molecules. Preventing degradation requires minimizing preparation time, maintaining low temperatures, and using appropriate inhibitor cocktails.Reproducibility is also critical: biological experiments require multiple replicates, and variability is often unavoidable. Careful handling, protocol optimization, and continuous troubleshooting are essential to ensure reliable results, whether preparing samples for chromatography, mass spectrometry, sequencing, or immunoprecipitation.<h5>Advances in Chromatography and MS for Malaria Research</h5>Modern advances in chromatography and mass spectrometry significantly enhance malaria research by enabling precise target identification, quantification, and functional characterization. Drug discovery workflows begin with construct design and recombinant expression, followed by purification and interaction studies.In malaria research, stage-specific parasite biology requires careful extraction and analysis of proteins from different life cycle stages. Tandem affinity MS, immunoprecipitation, and in vivo validation studies help confirm target relevance and therapeutic potential.Recent work on repurposed kinase inhibitors has shown promising results, including inhibition of hemozoin formation—a key detoxification pathway for the malaria parasite—highlighting the potential of such compounds for further development.<h5>Malaria, Drug Resistance, and Global Impact</h5>Malaria remains a major global health burden, with over 600,000 deaths annually and widespread drug resistance complicating treatment. Artemisinin resistance, particularly associated with mutations in the K13 propeller domain, has driven the search for new targets and therapies.During her PhD, Subhoja focused on falcipain-2, a cysteine protease involved in hemoglobin degradation within the parasite. This pathway is critical for parasite survival and has emerged as a promising drug target. Her work revealed that falcipain-2 operates within a larger protein complex responsible for hemoglobin processing and amino acid release.Understanding these molecular mechanisms is essential for developing robust, stage-specific antimalarial strategies.<h5>Advice for Early-Career Researchers</h5>For researchers just starting out, Subhoja emphasizes the importance of hands-on experience. Working directly with cells, lysates, and analytical tools is essential for truly understanding experimental systems. Failure is an inherent part of research, and perseverance is key.Motivation, teamwork, careful sample handling, and thorough preparation all contribute to long-term success. Reading extensively, critically evaluating one’s own work, and appreciating incremental progress are vital aspects of a sustainable research career.Every successful experiment—no matter how small—represents meaningful progress and contributes to the broader goals of science and medicine.<h5>Closing Remarks</h5>The discussion highlights how interdisciplinary approaches combining chromatography, mass spectrometry, structural biology, and biochemistry are driving advances in malaria drug discovery. Through careful experimental design, analytical rigor, and collaborative teamwork, researchers can address complex biological challenges and contribute to global health solutions.<blockquote>This text has been automatically transcribed from a video presentation using AI technology. It may contain inaccuracies and is not guaranteed to be 100% correct.</blockquote><blockquote><h4>Concentrating on Chromatography Podcast</h4>Dive into the frontiers of chromatography, mass spectrometry, and sample preparation with host David Oliva. Each episode features candid conversations with leading researchers, industry innovators, and passionate scientists who are shaping the future of analytical chemistry. From decoding PFAS detection challenges to exploring the latest in AI-assisted liquid chromatography, this show uncovers practical workflows, sustainability breakthroughs, and the real-world impact of separation science. Whether you’re a chromatographer, lab professional, or researcher you'll discover inspiring content!You can find Concentrating on Chromatography Podcast in podcast apps:<ul><li><a target="_blank" rel="noopener noreferrer" href="https://open.spotify.com/show/1wZg67rCjoGju4yhqxI3tL">Spotify</a></li><li><a target="_blank" rel="noopener noreferrer" href="https://podcasts.apple.com/tz/podcast/concentrating-on-chromatography/id1788429185">Apple podcast</a></li><li><a target="_blank" rel="noopener noreferrer" href="https://podscan.fm/podcasts/concentrating-on-chromatography">podscan</a></li></ul>and on <a target="_blank" rel="noopener noreferrer" href="https://www.youtube.com/@ChromatographyTalk/videos">YouTube channel</a></blockquote>

Affinity, Gel Filtration, Size Exclusion Chromatography: Repurposing Kinase Inhibitors for Malaria

Article

Product

Video

Scientists

Food

Interview

Science and research

The Institute of Organic Chemistry and Biochemistry of the Czech Academy of Sciences (IOCB Prague) is a leading scientific institution in the Czech Republic, recognized internationally. Its primary mission is basic research in the fields of chemical biology and medicinal chemistry, organic and material oriented chemistry, chemistry of natural compounds, biochemistry and molecular biology, physical chemistry, theoretical chemistry, and analytical chemistry. 

Institute of Organic Chemistry and Biochemistry of the Czech Academy of Sciences

Organomation, founded in 1959, designs and manufactures high-quality nitrogen evaporators and extraction systems. Known for innovation and durability, their lab instruments are used globally for efficient sample preparation with strong customer support.

Organomation

WATREX Prague is the distributor and producer of HPLC techniques in the Czech Republic and Slovakia. Main Chromatografic and Related Techniques Supported: LC, GPC, IC, IEC, Automated SPE, LC Columns and special column packings, LC method development, Software development, Laboratory Robots.

Watrex Praha

Technology Networks

Phenomenex

Thermo Fisher Scientific

Waters Corporation

<h3>Solving Problems That Matter - a Waters webinar series featuring USP speakers</h3><h4>Episode 8: Lipid nanoparticles</h4><div class="summary-section section">Developing safe, compliant and efficacious lipid nanoparticles (LNP) requires adequate testing of the individual lipid components and impurities. In this webinar Anni Chang from the USP Excipient team will share current and future excipient solution approaches and describe how the USP provides support for stakeholders in discovery and early-stage development. Then Duanduan Han will discuss the benefits of mass detection for critical impurities analysis, and Empower software workflows for compliant and straightforward method migration from development to QC labs. Duanduan will also highlight how multi-angle light scattering (MALS) detection can be used to characterize the LNP.<h5>Key learning objectives</h5><ul><li>Explore current and future USP solutions for lipid nanoparticles (LNP)</li><li>Learn how mass detection and Empower software-based workflows expedite and enhance routine testing of LNP components and impurities</li><li>Discover orthogonal insights enabled by multi-angle light scattering detection (MALS)</li></ul><h5>Who should attend</h5>Scientists involved with lipid nanoparticle analysis<blockquote>Presenter: Anni Chang, Ph.D. (Sr Scientist II, Excipients Unit, US Pharmacopeia)Dr. Anni Chang is currently a Senior Scientist II in the Excipients unit at the United States Pharmacopeia (USP). In her current role, she is responsible for developing, revising, and modernizing USP monographs. One of her focus areas is lipid excipients used in nanomaterial carriers.Dr. Chang has more than 15 years of experience in the biotech/pharmaceutical industry in analytical method development, validation, and transfer. She received her Ph.D. in Chemistry from University of Maryland, College Park, Maryland, USA. Dr. Chang is also active in the American Association of Pharmaceutical Scientists and serves as a Member Engagement Manager in the Lipid Based Drug Delivery community.Presenter: Duanduan Han, Ph.D. (Senior Scientist QA/QC Biologics Solutions group, Waters Corporation)Duanduan is a Senior Scientist in the QA/QC Biologics Solutions group at Waters Corporation headquarters in Milford, MA. She joined Waters in 2021, and she has focused on LC method development in composition analysis, impurity profiling, and raw material screening of lipid nanoparticle components and products. Additionally, she has explored other subjects of pharmaceutical analysis including surfactant, protein, and peptide. Prior to joining Waters, she received her PhD in chemical engineering from Texas A&amp;M University.Presenter: Panelist: Martin Kurnik, Ph.D. (Senior Scientist, Analytical Sciences, Waters | Wyatt Technology​)Martin Kurnik is a senior scientist in the Analytical Sciences team at Waters | Wyatt Technology. He helps customers identify and develop the best solutions for their analytical needs by leveraging his expertise in multi-angle light scattering and dynamic light scattering as well as a wide range of other leading techniques for biomolecular characterization. His postdoctoral research in protein folding and misfolding biophysics focused on understanding and preventing misfolding in bulk solution and on artificial surfaces used in medicine and biotechnology, and he has also developed protein- and aptamer-based electrochemical biosensors for continuous real-time measurements in vitro and in vivo. Martin received his M.Sc. in Chemistry and Ph.D. in Biochemistry from Stockholm University, Sweden.Moderated by: Isabelle Vu Trieu (Pharmaceutical Market Development Manager, Americas, Waters Corporation)Isabelle is a business development professional dedicated to delivering benefits to analytical scientists in the life sciences industry. With over 20 years of experience in providing chromatography and mass spectrometry solutions, Isabelle is particularly interested in helping pharmaceutical scientists achieve the data quality and integrity needed to drive decisions for drug quality, safety and efficacy. Isabelle joined Waters in 2009 and currently manages the development of the pharmaceutical market in the Americas. She holds Masters’ degrees in Chemistry from Columbia University and the University Claude Bernard in Lyon, France as well as a Chemical Engineering degree from Ecole Supérieure de Chimie Physique Electronique in Lyon, France.</blockquote></div>

Waters Corporation: Solving Problems That Matter - a Waters webinar series featuring USP speakers

Lipid nanoparticles

Solving Problems That Matter - a Waters webinar series featuring USP speakers. Episode 8: Complex excipients: Lipid nanoparticles.

Complex excipients: Lipid nanoparticles

Learn how Light Scattering can benefit your drug development!
The scientific community aims to enhance transparency and reliability in published research, with a specific focus on the quality of biological reagents, notably recombinant proteins. Concerns have been raised regarding irreproducible results, prompting discussions on common quality issues associated with recombinant proteins and their impact on downstream applications. In response, professionals in protein purification and characterization have collaboratively developed guidelines for improved quality control.
These guidelines include minimal information requirements for protein identity, production parameters, and long-term stability, as well as a minimal set of quality tests for purity, homogeneity, and identity. Additional recommendations cover DNA contamination, “spectral and thermal denaturation signatures”, homogeneity, “competent fraction”, storage conditions, batch-to-batch reproducibility, and other factors based on intended applications. 
Evaluation of these guidelines over a one-year period suggests that their implementation can enhance experimental reliability and optimize protein quality. Investing in protein QC benefits all stakeholders in life sciences, including researchers, editors, and funding agencies, by improving data accuracy and minimizing resource wastage
Key learning topics:
<ul>
<li>The latest guidelines for Quality control of Protein purification and characterisation 
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<li>How these guidelines can enhance reliability and improve protein quality in your labs
</li>
<li>How to reduce wastage and improve data accuracy in protein quality control
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</ul>
Who should attend?
<ul>
<li>Lab Managers and Scientists working in upstream recombinant protein production and QC
</li>
<li>Lab Managers and Scientists working in downstream protein purification QC
</li>
</ul>
<blockquote>
Presenter: Dr. André Matagne (Full Professor, University of Liège, Belgium)
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Dr. Matange is a Full Professor at the University of Liège, Belgium, where he is the chair at the Centre for Protein Engineering within the Faculty of Sciences.
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With a background in biochemistry, his research is primarily focused on proteins, particularly exploring their folding, stability, and biological activity. Notably, he has played a pivotal role in the development of Robotein®, an advanced technological platform designed for to high throughput protein expression, purification, formulation, and analysis.
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<blockquote>
Since 2016, Dr.Matagnehas been honored as an elected titular member of the Royal Academy of Sciences in Belgium.
</blockquote>

Waters Corporation: Quality Control of Purified Proteins to Improve Research Data Reproducibility: Improving the Time-Efficiency and Quality of Your Results​

Quality Control of Purified Proteins to Improve Research Data Reproducibility: Improving the Time-Efficiency and Quality of Your Results​

Learn how Light Scattering can benefit your drug development.

Quality Control of Purified Proteins to Improve Research

The sample throughput of high-sensitivity liquid chromatography-mass spectrometry (LC-MS) based proteomics analysis is restricted by the pressure limitations of LC and column hardware, with some methods wasting as much as 50% of the method cycle time outside of MS acquisition.
Traditional routes for boosting throughput include shorter columns and gradients, higher flow rates, and using trap-and-elute workflows – all of which introduce trade-offs in sensitivity and proteome depth.
The Thermo Scientific™ Vanquish™ Neo tandem direct injection workflow leverages hardware innovations and software intelligence to eliminate method overhead while maintaining data quality across nano, capillary, and micro-flow LC-MS.
In this webinar experts from Evotec and Bayer will showcase how they implemented the tandem direct injection workflow for their proteomics work. Dr. Nagarjuna Nagaraj will present on how Evotec is using tandem direct injection with nano-LC columns packed in-house to boost sample throughput of their chemoproteomics screening pipeline, while Dr. Alexander Boychenko from Bayer will discuss how his lab is using a micro-flow tandem direct injection approach to analyze plasma samples for translational proteomics.
Learning Objectives
<ul>
<li>See how minimizing LC-MS method overhead can accelerate sample throughput while maintaining proteome depth
</li>
<li>Discover how labs are using tandem direct injection for chemoproteomics and translational proteomics applications
</li>
<li>Learn how to implement tandem direct injection in your proteomics workflow
</li>
</ul>
<blockquote>
Presenter: Dr. Nagarjuna Nagaraj (Group Leader High Sensitivity Proteomics, Evotec)
</blockquote>
<blockquote>
Dr. Nagarjuna Nagaraj is a proteomics group leader at Evotec focusing on high sensitivity and high throughput proteomics applications with more than 18 years of experience in proteomics and mass spectrometry. Nagarjuna obtained his PhD from the Matthias Mann lab focusing on sample preparation and MS data acquisition strategies for clinical applications. After a postdoc he directed the mass spectrometry core facility at the Max-Planck-Institute of Biochemistry working on a wide range of protein and peptide mass spectrometry projects such as bottom-up proteomics, structural mass spectrometry including HDX, native and crosslinking MS, intact protein and small molecule analysis.
</blockquote>
<blockquote>
Presenter: Dr. Alexander Boychenko (Head of R&amp;D Laboratory Precision Medicine Markers, Bayer)
</blockquote>
<blockquote>
Alexander Boychenko is leading Omics Research within the Translational Sciences team of the Pharmaceuticals division at Bayer Group. He is focused on implementing innovative analytical technologies and developing novel methodologies for proteomics, metabolomics, and ligand-binding assays (LBA) to comprehensively characterize human and animal model phenotypes, enabling disease understanding and novel biomarker discovery. He holds a Ph.D. in Analytical Chemistry from Kharkiv National University, Ukraine. During his post-doctoral work at the University of Groningen, The Netherlands, he combined LC-MS and immunoassays to discover and validate protein biomarkers in biofluids for cancer screening. Before joining Research and Early Development at Bayer AG, he developed and introduced new liquid chromatography-mass spectrometry products at Thermo Fisher Scientific. Alexander is a subject matter expert in LC-MS, biological data interpretation, and precision medicine. He utilizes his passion for applying technical expertise to develop new medicines.
</blockquote>
<blockquote>
Presenter: Dr. Alec Valenta (Product Marketing Manager, Thermo Fisher Scientific)
</blockquote>
<blockquote>
Alec is the product marketing manager for the Vanquish Neo UHPLC system and low-flow LC columns. Prior to joining Thermo Fisher Scientific he earned his PhD in analytical chemistry from the University of Michigan where he helped advance microdialysis sampling of neurochemicals and developed LC-MS methods to quantify small molecule neurotransmitters, neuropeptides, and metabolites in a variety of complex biological samples.
</blockquote>

Thermo Scientific: A Tandem LC Workflow for Maximizing Mass Spectrometer Utilization in Proteomics

A Tandem LC Workflow for Maximizing Mass Spectrometer Utilization in Proteomics

How experts from Evotec and Bayer implemented the tandem direct injection workflow for their proteomics work.

A Tandem LC Workflow for Maximizing Mass Spectrometer

Join us for an exclusive two-day event where Agilent will showcase cutting-edge biopharma solutions.
The first day of the event we will explore Agilent’s comprehensive portfolio for the spectrum of applications from drug discovery to quality control. Learn more about our cell analysis and genomics expertise and the tools we provide to drive IVT mRNA development, protein therapeutics and cell and gene therapies
The second day will focus on advanced analytical solutions, including HRMS for tRNA sequencing and LC/MS for DNA/RNA analysis. Learn how to scale up oligonucleotide purification with Agilent biocolumns, which enhance the efficiency and accuracy of your processes.
By attending this event you will:
<ul>
<li>Gain insights from Agilent to optimize biotherapeutic workflows, including rigorous quality testing in cGMP environments and handling nucleic acid, vaccines, mAB and protein samples.
</li>
<li>Understand how metabolic analyses can impact CAR cell function and behavior, using cytolytic cell killing assays on the xCELLigence system to develop cell therapy processes and confirm product potency.
</li>
<li>Learn about new techniques in high-resolution LC/MS for analyzing tRNA modifications.
</li>
<li>Discover software tools available for high-resolution MS systems in both research and QA/QC labs.
</li>
<li>Compare analytical and semipreparative LC purification methods for synthetic oligonucleotides, gaining competitive insights into purification strategies.
</li>
</ul>
<h5>Talk 1: Agilent Tools and Technologies to Drive Advancements in Biopharmaceutical Drug Development</h5>
<ul>
<li>Presented by Dr. Hervé Chaulet, Molecular and Biological Research Market Specialist, Agilent Technologies</li>
</ul>
Discovering, developing, and manufacturing biopharmaceuticals requires you to stay abreast of advances in knowledge and improvements in technology while constantly navigating a maze of business and scientific challenges. Agilent can be a trusted partner providing expertise and end-to-end workflows including systems, software, consumables, and services for the spectrum of applications from drug discovery to quality control. Learn more about our cell analysis and genomics expertise and the tools we provide to drive IVT mRNA development, protein therapeutics, and cell and gene therapies.
<h5>Talk 2: Quality Control In IVT mRNA Therapeutics and Vaccine Development Workflow Using The Agilent Fragment Analyzer System</h5>
<ul>
<li>Presented by Dr. Leslie Friedmann, Product Manager, Agilent Technologies</li>
</ul>
Reliable and robust analysis is essential throughout in vitro transcription (IVT) mRNA workflows, providing important quality information to help minimize risk and aid in the production of a consistent product. The Agilent Fragment Analyzer systems can be used for several QC steps at different checkpoints in mRNA vaccine development, providing accurate and precise sizing and integrity analysis. These QC steps are to determine the quality and size of the linearized plasmid, the size and purity of the IVT mRNA, length of the poly(A) tail, and purity of the final mRNA vaccine product.
<h5>Talk 3: Analysis of NIST mAb Reference Material by Parallel SDS-Capillary Electrophoresis</h5>
<ul>
<li>Presented by Dr. Kyle Luttgeharm, Product Manager, Agilent Technologies</li>
</ul>
Monoclonal antibody drug products are complex biomolecules that must be tested for critical quality attributes (CQAs) such as monomeric purity and percent glycosylation. Capillary gel electrophoresis methods have been adopted as the standard method for size heterogeneity characterization, however legacy capillary electrophoresis (CE) methods involve complicated workflows or are cost prohibitive. To address these problems with legacy methods, Agilent has developed a 12-channel, parallel capillary electrophoresis-SDS (CE-SDS) instrument and reagents. The system uses a high-resolution sieving gel, allowing for resolution of mAb species including the non-glycosylated heavy chain and light chain. To demonstrate the capabilities of the ProteoAnalyzer, the NIST mAb was characterized under both reduced and non-reduced conditions and compared to the NIST data sheet.
<h5>Talk 4: Advanced Analytics for Cell Therapy Research, Process Development, and Manufacturing</h5>
<ul>
<li>Presented by Dr. Kelly Kroeger, Science &amp; Technology Advisor, Agilent Technologies</li>
</ul>
With immunology and immune-based therapies rising as a pillar of science and therapeutics, we are seeing a foundational shift from targeting specific proteins and pathways to actually targeting the immune cellular network that resolves and prevents disease. Therefore there is a critical need for cell analysis tools to facilitate understanding of the dynamic nature of the interaction of these cellular networks whether it is in the context of immune cells or immune cells with cancer cells.
To address these new challenges, we at Agilent Technologies have developed a portfolio of cell analysis solutions that provide researchers with more relevant tools to measure living cells, dynamically track them, and model their microenvironments. These next-generation therapies require methods and technologies to enable assessment of real-time cell function, phenotype, and fate. Used together, the Agilent tool bench provides the capability to measure and develop immune cell function more effectively, thus enabling translational researchers and developers to achieve the necessary level of potential therapeutic potency and safety.

Technology Networks: Biopharma Innovations Summit

Biopharma Innovations Summit Day 1

Learn more about our cell analysis and genomics expertise and the tools we provide to drive IVT mRNA development.

Join us for an exclusive two-day event where Agilent will showcase cutting-edge biopharma solutions.
The first day of the event we will explore Agilent’s comprehensive portfolio for the spectrum of applications from drug discovery to quality control. Learn more about our cell analysis and genomics expertise and the tools we provide to drive IVT mRNA development, protein therapeutics and cell and gene therapies
The second day will focus on advanced analytical solutions, including HRMS for tRNA sequencing and LC/MS for DNA/RNA analysis. Learn how to scale up oligonucleotide purification with Agilent biocolumns, which enhance the efficiency and accuracy of your processes.
By attending this event you will:
<ul>
<li>Gain insights from Agilent to optimize biotherapeutic workflows, including rigorous quality testing in cGMP environments and handling nucleic acid, vaccines, mAB and protein samples.
</li>
<li>Understand how metabolic analyses can impact CAR cell function and behavior, using cytolytic cell killing assays on the xCELLigence system to develop cell therapy processes and confirm product potency.
</li>
<li>Learn about new techniques in high-resolution LC/MS for analyzing tRNA modifications.
</li>
<li>Discover software tools available for high-resolution MS systems in both research and QA/QC labs.
</li>
<li>Compare analytical and semipreparative LC purification methods for synthetic oligonucleotides, gaining competitive insights into purification strategies.
</li>
</ul>
<h5>Talk 1: Advanced Analytical Solutions for Nucleic Acid Analysis and Purification</h5>
<ul>
<li>Presented by Dr. Charlotte Lekieffre, Biopharma Application Specialist, Agilent Technologies</li>
</ul>
The emphasis during the second day of the Summit will be on advanced analytical solutions, such as HRMS for tRNA sequencing and LC/MS for DNA/RNA analysis. Additionally, you’ll discover techniques to enhance the efficiency and accuracy of your processes by scaling up oligonucleotide purification using Agilent biocolumns. 
<h5>Talk 2: Extracted Yeast tRNA Sequencing by LC-MS/MS</h5>
<ul>
<li>Presented by Aurore Attina, Engineer of Mass Spectrometry, University of Montpellier</li>
</ul>
RNA modifications are essential for RNA stability, maturation, and functionality; directly influencing protein translation and gene regulation. They play a central role in various biological processes and are associated with many diseases, including cancers and neurological disorders. tRNA (transfer RNA), which plays a key role in protein synthesis, is the most modified molecule in the organism known to date. tRNA modifications are essential to ensure their correct function in protein translation, maintain the accuracy and fidelity of the genetic code, and enable flexible adaptation to changing cellular conditions and are therefore a crucial attribute to monitor. To monitor and map these modifications of tRNA, we have developed a LC-HRMS analytical workflow, first using synthetic oligonucleotide for the method optimization. In this webinar, we will first go through the various steps involved in LC-MS/MS method optimization using synthetic oligonucleotides. In a second part, we will present the protocol development of an enzymatic digestion applied to tRNA extracted from yeast. This digestion generates oligonucleotide fragments that can be analyzed by LC-MS/MS for sequence confirmation.
<h5>Talk 3: Discover Agilent’s Software Tools to Confirm Oligonucleotide Sequence Integrity</h5>
<ul>
<li>Presented by Dr. Holger Stalz, LC/MS Product Specialist Austria &amp; Switzerland, Agilent Technologies</li>
</ul>
Mass spectrometry (MS) is increasingly important for the development of therapeutic modalities such as oligonucleotides. A common analytical method for large molecules is intact mass by LC/MS. This can be used for the characterization and identification of a product or process-related impurities, or simply as a confirmation of molecular weight. The newly introduced Agilent OpenLab CDS 2.8 SW version comes with a set of features to enable the automatization of biopharma workflows. If you want to know how LC/MSD Spectral Deconvolution of your biopharma samples can be automated, we will provide answers during our presentation. Even more details on Oligonucleotide sequence confirmation and impurities characterization can be acquired using the power of a high-resolution accurate mass system (HRAM) on the Agilent 6545XT AdvanceBio LC/Q-TOF. Analyzing the sample data with the software workflows in Agilent BioConfirm version 12 will support the user to get the trusted answers to their most relevant questions.
<h5>Talk 4 Maximize Your Resources and Simplify Scale-up with AdvanceBio Oligonucleotide Columns</h5>
<ul>
<li>Presented by Dr. Andrea Tripodi, Application Development Engineer, Agilent Technologies</li>
</ul>
Several classes of nucleic acids, such as antisense oligonucleotides, small interfering RNAs (siRNAs), and aptamers are being investigated for potential therapeutic applications. Synthetic oligonucleotides inevitably contain many impurities due to limitations in the efficiency of each chemical reaction used during manufacture. They include deletion sequences (missing one or more nucleotides) and reaction by-products often arising during the final deprotection or cleavage steps. Ion pair reversed-phase (IP-RP) chromatography stands out as the prevalent method for analyzing and purifying oligonucleotides. For increased resolution and separation of your full-length synthetic oligonucleotide from closely related impurities, AdvanceBio Oligonucleotide is the ideal choice. The latest developments include semi-preparative, and lab-scale preparative columns packed with superficially porous particles that provide excellent performance and resolution without compromising column loading. A fast and easy scale-up method from analytical to semi-preparative scale was successfully achieved using a 10 mm ID column enabling higher sample loading using the same analytical LC instrument.

Biopharma Innovations Summit Day 2

The allowable adjustments to compendial methods published in USP General Chapter 621 and Ph. Eur. 2.2.46 provide scope for analysts to migrate methods to smaller column dimensions, providing advantages in terms of time and cost savings.This webinar will provide insight into the considerations necessary when applying allowable adjustments to gradient monographs.<h4>Key Learning Points:</h4><ul><li>Learn about Allowable adjustments possible when working with gradient monograph methods</li><li>Understand system considerations in terms of dwell volume</li><li>Dig into calculations to perform in order to scale gradient conditions correctly</li></ul><h4>Who Should Attend:</h4><ul><li>Any analysts working with pharmacopeia methods or chromatographers who work with gradient methods&nbsp;</li></ul><blockquote>Presenter: James Turner (Global Market Manager, Phenomenex)With more than 25 years of experience at Phenomenex, James has been responsible for providing specialist technical support to customers across Europe with method development assistance, troubleshooting advice and chromatographic solutions to various customer problems. James has also been involved with the technical training of many Phenomenex technical consultants, across the multiple techniques we serve.</blockquote>

Phenomenex: Working with Allowed Adjustments Pharmacopeia HPLC Methods – Gradient Considerations

Working with Allowed Adjustments Pharmacopeia HPLC Methods – Gradient Considerations

Insight into the considerations necessary when applying allowable adjustments to gradient monographs.

Working with Allowed Adjustments Pharmacopeia HPLC

This seminar will guide you through troubleshooting HPLC systems and chromatographic issues using a structured workflow.We will share a novel approach to emphasize the importance of system monitoring, as troubleshooting without monitoring is considerably more challenging. After introducing some key concepts and a troubleshooting workflow, we'll explore practical examples by applying the workflow to failed chromatograms to identify the cause of failure and then use a test cycle to confirm the fix.Who should attend: Analysts working in regulated environments (eg. GLP, GMP) or in non-regulated environments (eg. R&amp;D) who want to troubleshoot their HPLC systems and chromatographic problems effectively.<h4>Key Learnings:</h4>1. The importance of routine system monitoring2. Using qualification checks to diagnose a failure3. Understanding Failure Indicators (HOW the chromatogram failed) vs. Failure Causes (WHY the chromatogram failed)4. Using troubleshooting workflows to quickly identify the failure cause<blockquote>Presenter: Maureen DeLoffi (Senior Product Manager, Waters COrporation)Maureen DeLoffi is a Senior Product Manager at Waters Corporation, where she manages the small molecule portfolio of Analytical HPLC columns. Prior to her Product Manager role, she spent over 12 years as a scientist in the Column Technology Development Lab at Waters. She holds a bachelor’s degree in Mechanical Engineering from the University of New Hampshire.</blockquote>

Waters Corporation: HPLC Troubleshooting: A System Monitoring Approach

HPLC Troubleshooting: A System Monitoring Approach

Troubleshooting HPLC systems and chromatographic issues using a structured workflow.

The use of biotherapeutic synthetic peptides in drug development has grown significantly in the past decade.Biotherapeutic synthetic peptides often have higher efficacy, safety, and tolerability in humans compared to small molecules. &nbsp;They also have lower production complexity and cost than protein-based biopharmaceuticals.While synthesis reactions are carefully controlled, impurities as well as degradation species are inevitably formed and must be characterized and identified. Having a robust Reversed-Phase (RP) method provides a solid foundation in the characterization and identification of these impurities. &nbsp;This webinar will review some of the principles and practices to develop robust RP separation methods showing examples using GLP-1 Agonists.<ul><li>Principles of Reversed-Phase LC and LC/MS of Peptides</li><li>Considerations in Selecting an Appropriate RP Column</li><li>Considerations in Selecting an Appropriate RP Eluent</li><li>Step by Step Approach to Developing a Robust Peptide Separations</li></ul><blockquote>Presenter: Bill Warren (Principal Consultant Product Manager, Waters Corporation)In 1986, Bill joined Waters Corporation as a senior applications chemist involved with the research and development of HPLC, FPLC, and Capillary Electrophoresis applications for the analysis and purification of proteins, peptides, and nucleic acids.He currently is a Principal Consulting Bio separations Product Manager whose responsibilities involve assisting in the development and commercialization of innovative reagents and chromatography-based technologies for the separation, purification, and characterization of proteins and peptides.</blockquote>

Waters Corporation: Development of Reversed-Phase Methods for the Analysis of Biotherapeutic Synthetic Peptides including GLP-1 Agonists

Development of Reversed-Phase Methods for the Analysis of Biotherapeutic Synthetic Peptides including GLP-1 Agonists

This webinar will review some of the principles and practices to develop robust RP separation methods showing examples using GLP-1 Agonists.

RP Methods for the Analysis of GLP-1 Agonists

<h4>The Waters 2024 Virtual Summer School - An Educational Journey to Master Food &amp; Environmental Analysis</h4>Unlock the full potential of LC-MS techniques with our immersive and educational sessions at the Waters Virtual F&amp;E Late Summer School 2024. From September 24th to 26th, engage with leading experts and discover cutting-edge methods for environmental and food analysis.<h5>Why attend?</h5><ul><li>Comprehensive Learning: Dive deep into advanced LC-MS techniques and gain practical knowledge to enhance your laboratory's analytical capabilities.</li><li>Expert Insights: Learn from industry leaders and gain firsthand experience through detailed modules on chromatography, mass spectrometry, and sample preparation.</li><li>Regulatory Compliance: Stay ahead with the latest insights on EU regulations for PFAS analysis in food and water, ensuring your lab meets stringent standards.</li></ul><h3>Episode #1: Chromatography - UPLC | F&amp;E Summer School - Day 1 - Module 1</h3>This section will provide an overview of the Waters reference material available to customer to help plan the best columns and chromatographic conditions for their LC-MS methods, using common environmental contaminants as an example. We highlight how to create LC-MS methods using the Acquisition Method Editor (AME) application within waters_connect™. Finally, this section will cover some of the most troubleshooting issues: contaminated blank peaks, poor peak shape, etc, and how to address them.<h5>What you'll learn:</h5><ul><li>Important factors for developing a UPLC method - considering analyte solubility, pKa and partitioning coefficient (Kow)</li><li>Selecting the most appropriate UPLC column for your analytes</li><li>Setting up LC-MS methods in the waters_connectTM console</li><li>Troubleshooting common contamination issues</li></ul><blockquote>Presenter: Nicole Baumgarten (Sr Sales Applications Chemist, Waters Corporation)Nicole is part of the Customer Success team at Waters with a focus on LC-MS/MS application and sales support. She works in this role for more than 9 years and gained experience in previous roles as analyst for biopharmaceuticals in process controls at Boehringer Ingelheim and research Assistent at Zentrum für Humangenetik und Laboratoriumsmedizin in Munich after finishing a Master of Chemical engineering at Hochschule Fresenius, Idstein.Presenter: Caroline Davis (Sr Sales Applications Chemist, Waters Corporation)</blockquote>

Waters Corporation: The Waters 2024 Virtual Summer School

Chromatography - UPLC

An overview of the Waters reference material available to customer to help plan the best columns and chromatographic conditions for their LC-MS methods.

Chromatography - UPLC 

<h4>Overview</h4>We invite you to our 2nd Annual Virtual Biosymposium. This year, we will explore novel analytical characterization and quantitation of emerging modalities, such as DNA and mRNA, along with key method development considerations with global thought leaders.We look forward to seeing you! Key Event Objectives:<ul><li>Learn about novel approaches to characterizing established and modern biotherapeutic modalities</li><li>Understand key considerations when developing characterizing and quantitating methods for biomolecules</li><li>Take a deep dive into the values of fit-for-purpose methods to make critical drug decisions</li></ul>AGENDA7:30am AM (PT) - Welcoming Introductions<ul><li>Tran N. Pham, Global Biopharma Market Development Manager at Phenomenex</li></ul>7:35 AM (PT) - "Solutions &amp; Characterization Strategies for IVT mRNA Therapy Development"<ul><li>Charles Bai, Lead Application Scientist, VectorBuilder, Chicago, USA</li></ul>8:05 AM (PT) - &nbsp;"Utilizing Size Exclusion Chromatography for the Quality Control Analysis of Gene Therapeutics”<ul><li>Rebecca Hirschberger, QC Manager Bioanalytical Implementation, and Dr. Christopher Gawlig, QC Manager Research and development, Biospring, Germany</li></ul>8:35 AM (PT) "Impurity Profiling Optimization of GalNAc-conjugated Oligonucleotides by LC-MS-UV"<ul><li>Roxana Eggleston-Rangel, Senior Applications Scientist, Phenomenex, California, USA</li></ul>9:05 AM (PT) – Round Table Q&amp;A<ul><li>Tran N. Pham, Global Biopharma Market Development Manager at Phenomenex</li></ul>9:25 AM (PT) – Closing Remarks<blockquote>Presenter: Charles Bai (Lead Application Scientist)Charles has received his PhD in Microbiology at Purdue University. As a Lead Application Scientist at VectorBuilder, his main responsibilities are to provide technical consultation for clients regarding gene delivery using viral and non-viral vectors. Regarding the application of in vitro transcribed (IVT) RNA, Charles has been helping researchers with the design, production and encapsulation of IVT mRNA, circRNA or saRNA for therapy and vaccine development.Presenter: Rebecca Hirschberger (Quality Control Bioanalytical Method Development)Rebecca has been in the chromatography industry for over 2 years and has practical experience in the quality control of gene therapeutics. She has earnt a Masters in pharmaceutical science from Ludwig-Maximilians University, Munich, focused on instrumental analytics. Since Dezember 2022 she is a licensed Pharmacist in Germany. Rebecca Hirschberger joined BioSpring in August of 2021 and she serves as QC Manager Bioanalytical Implementation. Her area of responsibility includes: Various analyses of oligonucleotides, including (tandem) mass spectrometry via qToF, biochemical techniques, i.e. ELISA, enzymatic transformation of oligonucleotides for analytical and quality control purposes and the Separation of oligonucleotides by chromatographic methods.Presenter: Roxana Eggleston-Rangel (Senior Applications Scientist, Phenomenex)Roxana joined Phenomenex in 2018 as an Applications Scientist and is currently working with the applications team to produce analytical solutions for scientist in the biopharma industry. Roxana’s current work focuses on characterization of mRNA, sgRNA and siRNA. Prior to Phenomenex, Roxana worked for Caltech and the University of Southern California developing LM-CS workflows for the proteomics community. Roxana Eggleston-Rangel received her bachelor’s and master’s degree in Chemistry and Biochemistry from California State University, Los Angeles.</blockquote>

Phenomenex: 2nd Annual Virtual Biosymposium

2nd Annual Virtual Biosymposium

Explore novel analytical characterization and quantitation of emerging modalities, such as DNA and mRNA

LCMS webinars focusing on HPLC - page 19

Biopharma Innovations Summit Day 2

HPLC Troubleshooting: A System Monitoring Approach

Biopharma Innovations Summit Day 1

Chromatography - UPLC

Lipid nanoparticles

2nd Annual Virtual Biosymposium

Working with Allowed Adjustments Pharmacopeia HPLC Methods – Gradient Considerations

A Tandem LC Workflow for Maximizing Mass Spectrometer Utilization in Proteomics

Quality Control of Purified Proteins to Improve Research Data Reproducibility: Improving the Time-Efficiency and Quality of Your Results

Development of Reversed-Phase Methods for the Analysis of Biotherapeutic Synthetic Peptides including GLP-1 Agonists

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LCMS webinars focusing on HPLC - page 19

Biopharma Innovations Summit Day 2

HPLC Troubleshooting: A System Monitoring Approach

Biopharma Innovations Summit Day 1

Chromatography - UPLC

Lipid nanoparticles

2nd Annual Virtual Biosymposium

Working with Allowed Adjustments Pharmacopeia HPLC Methods – Gradient Considerations

A Tandem LC Workflow for Maximizing Mass Spectrometer Utilization in Proteomics

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Development of Reversed-Phase Methods for the Analysis of Biotherapeutic Synthetic Peptides including GLP-1 Agonists

Related content

Affinity, Gel Filtration, Size Exclusion Chromatography: Repurposing Kinase Inhibitors for Malaria

Research from IOCB Prague reveals a previously unknown mechanism of genetic transcription

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