<div>Purifying the target protein is crucial for investigating its specific properties and interactions with biomolecules like DNA, RNA, and other proteins. A range of purification strategies is available, and the optimal approach depends on factors such as the required scale, throughput, and downstream application. In many cases, the process must be fine-tuned through empirical optimization to achieve the desired purity and yield.</div><div><div><div><div><h3>What is Protein Purification?</h3>Protein purification is a critical process in biotechnology and life sciences, aimed at isolating a specific protein from complex biological mixtures such as cell lysates or tissue extracts. The goal is to obtain a highly purified protein sample, free from contaminants like other proteins, nucleic acids, and lipids, to enable accurate analysis of its structure, function, and interactions.This process is especially vital in biotechnological areas inside immunology and microbiology. In immunology, it underpins the production of vaccines, therapeutic antibodies, and diagnostic assays by enabling the isolation of key immune proteins and antibodies. It also supports the investigation of cell signaling and immune response mechanisms. In microbiology, purified proteins are essential for studying pathogens, identifying disease-related antigens, and driving drug discovery efforts. Ultimately, protein purification is fundamental to the development of targeted vaccines, innovative diagnostics, and effective therapeutic strategies.</div></div></div></div><div><div><div><div><h3>Types of Protein Purification Methods</h3></div></div></div></div><div>There are several types of protein purification methods, each chosen based on the protein’s characteristics such as size, charge, solubility, and binding affinity. Below are some commonly used methods:</div><div><div><div><div><h4>Non-Chromatographic Approaches</h4></div></div><div>Non-chromatographic approaches use simpler physicochemical principles—such as solubility, molecular size, or diffusional properties—to separate and purify proteins. These techniques are often employed as initial purification or concentration steps before further refinement.<h5>Precipitation Techniques</h5>Protein precipitation is a widely used approach in purification workflows, typically relying on one of three main methods:<ul><li>Organic solvent precipitation involves adding solvents like ethanol or acetone to lower the solution's dielectric constant. This promotes protein–protein interactions, reducing solubility and leading to aggregation and precipitation.</li><li>Isoelectric point precipitation exploits the pH at which a protein carries no net charge. At this point, solubility is minimized, causing the protein to precipitate from solution.</li><li>Nonionic polymer precipitation uses polymers such as dextran, polyethylene glycol (PEG), or NPEP to alter the solution’s physical properties. These agents create crowding effects that promote protein aggregation without directly binding to the proteins.</li></ul><h5>Dialysis</h5>Dialysis involves placing a protein solution inside a semi-permeable membrane that allows water and ions to pass while the protein remains inside, facilitating buffer exchange.This method is commonly used during protein expression and purification workflows for buffer exchange, desalting, and removal of unwanted small molecules such as imidazole, urea, or ammonium sulfate. The process helps maintain protein stability and activity by gradually equilibrating the sample with the desired buffer conditions.<h5>Immunocapture (Magnetic Bead-Based Purification</h5>Immunocapture uses antibodies immobilized on solid supports like magnetic beads to selectively bind and isolate a target protein. This method is highly specific and particularly useful for low-abundance targets or small-scale prep, such as:<ul><li>Pulling down proteins from complex mixtures</li><li>Capturing tagged recombinant proteins (e.g., FLAG, HA, or His tags)</li><li>Enrichment before western blotting, MS, or ELISA</li></ul>Magnetic beads offer speed, ease of use, and minimal sample loss, making them ideal for lab-scale workflows.<a target="_blank" rel="noopener noreferrer" href="https://www.phenomenex.com/products/biozenspe-sample-preparation-products/biozen-magbeads">Biozen MagBeads</a> provide a fast and efficient method for purifying, isolating, and cleaning up monoclonal antibodies (mAbs), proteins, and peptides. These paramagnetic beads feature a streptavidin-coated surface, enabling high-affinity binding in immuno-based workflows. Compared to traditional sample preparation techniques, magnetic bead-based immunocapture offers greater speed and consistency by delivering high binding capacity with uniform particle size, ensuring accurate and reproducible results in significantly less time.<h5>Centrifugation</h5>Centrifugation of crude protein mixtures is an essential step in the investigation of protein purification and aggregation. Outlined below are the various types of centrifugations employed in protein purification.Preparative Ultracentrifugation Ultracentrifugation is an effective method of purifying proteins from aggregates based on their size, shape and density. Centrifugation, widely recognized as a robust and versatile method, is commonly used to separate complex mixtures of cellular components. Ultracentrifuges separate small particles by spinning at high speeds, utilizing centrifugal force based on size and density.Differential Centrifugation Differential centrifugation operates by separating components based on differences in size and density, using varying speeds of centrifugation to achieve this. Its resolving power ranges from low to medium. A key advantage of this technique is its convenience for processing multiple samples simultaneously, making it effective for purifying crude samples. Additionally, it preserves cell viability and maintains the natural distribution of hematopoietic cell populations.Rate-zonal Gradient Centrifugation This technique separates components based on their sedimentation rate within a medium that contains a density gradient. It offers medium resolving power and is particularly effective for separating multiple components in complex mixtures with high precision. It is also considered a cost-effective and widely used technique in molecular biology and biochemistry.Isopycnic Centrifugation (Equilibrium Centrifugation) Isopycnic centrifugation is an advanced protein purification method that relies on the principle of separating particles according to their intrinsic buoyant density, irrespective of their size or morphology. During the process, proteins migrate through a density gradient until they equilibrate at a position where their density matches that of the surrounding medium, resulting in precise separation without further sedimentation.</div></div></div><div><div><div><div><h4>Chromatographic Approaches</h4>Chromatography is a common method of protein purification and is based on the separation of molecules in a mixture as they move through a stationary phase and a mobile phase. Outlined below are the various types of chromatography employed in protein purification.<h5>Ion Exchange Chromatography</h5><a target="_blank" rel="noopener noreferrer" href="https://www.phenomenex.com/techniques/hplc-ion-exchange">Ion exchange chromatography</a> relies on the interaction between a charged stationary phase and oppositely charged analytes, with cation exchange chromatography attracting positively charged molecules to a negatively charged stationary phase. Successful application of this technique requires careful selection of the appropriate ion exchange column, buffer pH, and salt concentration. This approach is often used in the biopharmaceutical industries as mAb polishing steps, viral clearance and charge variant analyses as part of the product quality control.<a target="_blank" rel="noopener noreferrer" href="https://www.phenomenex.com/">Phenomenex</a> offers a range of protein purification columns. Their ion exchange chromatography solutions include:&nbsp;<ul><li><a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/products/1526">Luna SCX </a>for strong selectivity of positively charged compounds, such as peptides</li><li><a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/products/1540">Biozen WCX</a> for efficient separation of proteins such as monoclonal antibodies charged variants</li><li><a target="_blank" rel="noopener noreferrer" href="https://www.phenomenex.com/part/00g-3151-e0">PhenoSphere SAX</a>, a strong anion-exchanger with fully porous 80Å particles, particularly useful for small molecules such as nucleotides</li></ul><h5>Size Exclusion Chromatography</h5><a target="_blank" rel="noopener noreferrer" href="https://www.phenomenex.com/techniques/hplc-sec">Size exclusion chromatography (SEC)</a> separates molecules based on their size, with larger analytes eluting first due to their exclusion from the pores in the column packing material, while smaller molecules are able to enter the pores, facing a longer travelling path, and hence, eluting later. This method can be categorized into aqueous SEC (gel filtration chromatography, GFC) for analyzing biomolecules such as proteins and antibodies. This type of chromatography is widely used as an analytical tool for quality control in the biopharmaceutical industry and can also be an approach of choice for polishing steps.Phenomenex offers a range of Biozen columns for SEC separations. In the case of mAbs, <a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/products/1534">Biozen dSEC-2</a> and<a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/products/1537"> Biozen dSEC-7 </a>columns are the top choices for monoclonal antibody (mAb) analysis, delivering high-resolution separation of monomers, aggregates, and fragments formed during mAb manufacturing, processing, or storage. The dSEC-7 column, featuring a larger pore size and optimized particle design, extends the dynamic range to accommodate larger biomolecules and complex biotherapeutics.For more general applications, <a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/products/1533">Biozen SEC-2</a> and <a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/products/1532">Biozen SEC-3</a> phases cover different MW ranges for high-efficiency separation of biomolecules (e.g., proteins and antibodies). Additionally, the BioSep and Yarra lines become top choices when looking for preparative scale columns.<h5>Reversed-Phase Chromatography</h5><a target="_blank" rel="noopener noreferrer" href="https://www.phenomenex.com/techniques/hplc-reversed-phase">Reversed-phase chromatography</a>, the most common mode in high-performance liquid chromatography/ ultra-high-performance liquid chromatography (HPLC/UHPLC), separates compounds based on hydrophobic and van der Waals interactions using alkyl (C18, C8, C4) stationary phases or other hydrophobic polymers. For large biomolecules such as proteins and peptides, <a target="_blank" rel="noopener noreferrer" href="https://www.phenomenex.com/resources/blog/wider-pore-biozen-column">wide-pore</a> (typically 300 Å) C4, C8 and C18 columns are ideal, as the larger pore size allows better accessibility of the protein molecules to the stationary phase surface, improving mass transfer and peak shape during separation.The Biozen line from Phenomenex offers a wide range of HPLC/UHPLC columns for the analysis of biomolecules.These include phases optimized for high-resolution analysis of intact proteins (Native-RP, Widepore C4, Intact XB-C8) and peptides (Peptide PS-C18, Peptide XB-C18 and Peptide Polar-C18). Kinetex could also be an option when looking for more selectivities for the analysis of peptides and small proteins. Additionally, when aiming to preparative scale processes, several of these media are also available in Phenomenex Axia formats to easily up-scale your separations.<h5>Hydroxyapatite Chromatography</h5>Hydroxyapatite chromatography (HAC) is a sophisticated mixed-mode chromatographic technique that exploits the unique properties of hydroxyapatite (Ca10(PO4)6(OH)2), utilizing both cation-exchange interactions via its phosphate groups and metal affinity through its calcium sites to achieve highly selective protein separations.Renowned for its efficacy in resolving complex biomolecular separations, HAC excels in tasks like aggregate removal from monoclonal antibodies, surpassing the capabilities of traditional single-mode chromatographic methods such as ion-exchange or hydrophobic interaction chromatography.<h5>Hydrophobic Interaction Chromatography</h5>Hydrophobic interaction chromatography (HIC) is a method of protein purification that separates molecules based on their hydrophobicity, using high-salt buffers to promote protein-ligand interactions while maintaining the protein's biological activity. The process involves gradually reducing the salt concentration to elute proteins in order to increase hydrophobicity, making it effective for purifying target molecules and removing impurities or aggregates, making it a valuable approach for intermediate and polishing steps during purification processes.<h5>Affinity Chromatography</h5>Affinity chromatography is a powerful and highly selective technique used to purify biomolecules based on specific, reversible interactions between a target molecule and an immobilized ligand. In many cases, recombinant proteins are engineered with affinity tags—such as His-tags or GST-tags—that facilitate binding to complementary ligands on the chromatography matrix.This method offers the advantage of achieving several thousand-fold purifications in a single step while preserving the biological activity of the protein. It is widely employed for purifying enzymes, antibodies, and antigens, using a variety of ligands including specific antibodies, enzymes, and multifunctional compounds like concanavalin A and triazine dyes.</div></div></div></div>

Exploring Protein Purification Methods: Essential Techniques for Accurate Results

<h3>A Practical Guide to Getting the Most from Syringe Filters: Tips, Tricks, and Common Pitfalls</h3>Syringe filters play a crucial role in chromatography and many other laboratory applications by removing particulate matter before analysis. Proper sample filtration helps protect analytical instruments, extends column lifetime, and improves data quality. However, selecting an unsuitable filter—or using one incorrectly—can introduce contamination, reduce filtration efficiency, or even compromise analytical results. The following recommendations will help you avoid common mistakes and ensure reliable filtration every time.<img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/KNAUER_Avoiding_Common_Syringe_Filter_Mistakes_3_150e8c1c2b.webp" alt="KNAUER: Avoiding Common Syringe Filter Mistakes" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_KNAUER_Avoiding_Common_Syringe_Filter_Mistakes_3_150e8c1c2b.webp 133w," sizes="100vw" width="1276" height="1496"><h4>1. Improper Filter Handling</h4>Using excessive force during filtration or attempting to reuse disposable syringe filters can damage the membrane and increase the risk of sample contamination.<ul><li>Solution: Apply slow, even pressure throughout the filtration process and always dispose of single-use filters after one use. If the flow rate decreases noticeably, replace the filter instead of applying additional force.</li></ul><h4>2. Selecting an Inappropriate Pore Size</h4>Using the wrong pore size may either fail to remove unwanted particles or cause the membrane to clog prematurely, reducing filtration efficiency.<ul><li>Solution: A 0.45 µm membrane is generally suitable for routine particulate removal, while a 0.22 µm membrane is recommended for finer filtration and bacterial removal. Samples with higher viscosity may require a pre-filter to prevent rapid clogging. It is also important to consider the intended analytical technique. For example, 0.22 µm filters are typically preferred for UHPLC and LC-MS applications, whereas 0.45 µm filters are often sufficient for conventional HPLC analyses.</li></ul><h4>3. Choosing the Wrong Membrane Material</h4>Different membrane materials exhibit different chemical resistance. Using an incompatible membrane may lead to degradation of the filter, sample contamination, or complete filtration failure.<ul><li>Solution: Always verify solvent compatibility before filtration. PTFE membranes are an excellent choice for organic solvents, while PES and nylon membranes are better suited to aqueous samples. If compatibility is uncertain, perform a small-scale trial before filtering valuable samples.</li><li>Pro Tip: For additional guidance, see our blog article "How to Choose the Right Membrane for your Syringe Filter", which provides a detailed comparison of membrane materials and their recommended applications.</li></ul><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/KNAUER_Avoiding_Common_Syringe_Filter_Mistakes_2_e47021fb11.jpg" alt="KNAUER Sepapure® Syringe Filters are available with multiple membrane materials, each identified by a dedicated color code for quick and reliable selection." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_KNAUER_Avoiding_Common_Syringe_Filter_Mistakes_2_e47021fb11.jpg 245w," sizes="100vw" width="1580" height="902"><h4>4. Ignoring Filter Capacity</h4>Using a filter with insufficient capacity can result in rapid membrane blockage, increasing filtration time and reducing laboratory productivity.<ul><li>Solution: When filtering larger sample volumes, choose high-capacity syringe filters or introduce a pre-filtration step. Never force liquid through a clogged membrane, as doing so may damage the filter and negatively affect sample quality.</li></ul><h4>5. Overlooking Sample Adsorption</h4>Certain membrane materials may adsorb analytes of interest, leading to lower recoveries and potentially affecting quantitative results.<ul><li>Solution: For protein samples or other adsorption-sensitive analytes, select membranes with low binding characteristics, such as nylon or glass fiber, whenever appropriate. Performing a small recovery test before routine analysis can help identify potential analyte losses.</li></ul><h4>6. Failing to Optimize Filtration Performance</h4>Slow filtration is more than just an inconvenience—it often indicates that the filtration conditions are not optimal.<ul><li>Solution: If filtration becomes unusually slow, investigate possible causes such as membrane clogging, excessive sample viscosity, or an unnecessarily small pore size. Depending on the sample, dilution or sequential filtration using progressively finer filters may significantly improve performance.</li></ul><h4>Final Thoughts</h4>Selecting the appropriate syringe filter is a straightforward process, but overlooking small details can have a significant impact on analytical performance. Choosing the correct membrane material, pore size, and filtration strategy helps improve laboratory efficiency, protects chromatography systems and columns, and contributes to accurate, reproducible analytical results.With the right syringe filter and proper filtration technique, you can minimize sample preparation problems and maximize confidence in every analysis.<img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/KNAUER_Avoiding_Common_Syringe_Filter_Mistakes_4_983a15e289.jpg" alt="KNAUER: Sepapure® Syringe Filter Selection" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_KNAUER_Avoiding_Common_Syringe_Filter_Mistakes_4_983a15e289.jpg 245w," sizes="100vw" width="1160" height="627">Happy filtering!

Avoiding Common Syringe Filter Mistakes

News from LabRulezLCMS Library - Week 27, 2026

Article

Product

Application

Academy

Poster

Everything from the world of analytical chemistry in one place. We connect people in solving their problems. At Labrulez you will find all the necessary information easily, quickly and clearly. Stop searching and start finding.

LabRulez

Phenomenex is a global technology leader committed to developing novel analytical chemistry solutions that solve the separation and purification challenges of researchers in industrial, government and academic laboratories.

Phenomenex

Based in Berlin, KNAUER is a medium-sized, owner-managed company that has been serving the sciences since 1962. We develop and manufacture scientific instruments of superior quality for liquid chromatography, including: Analytical HPLC/UHPLC, Preparative HPLC, Fast protein liquid chromatography (FPLC), Multi-column chromatography/Simulated moving bed (SMB) chromatography. High pressure dosing, in-line detection systems, and osmometry are additional business areas.

KNAUER

New Food magazine

Agilent Technologies

Technology Networks

Thermo Fisher Scientific

SelectScience

Waters Corporation

Join this virtual panel to hear experts discuss new liquid chromatography technologies and techniques for separation of dietary supplements.
Health-conscious consumers are increasingly seeking supplements that deliver targeted health benefits. Meeting the demands of this growing market while maintaining consumer trust, cost control, and speed to market presents a host of analytical challenges.
Join us for an informative webinar focused on the evolving landscape of dietary supplements, where industry experts will discuss standard methods, advanced analytical techniques, and quality control. Learn how the standard methods are established and how to navigate common compliance challenges using advanced analytical techniques.
We will explore the latest chromatography technologies that improve the accuracy and reliability of supplement testing. Gain insights into strategies for ensuring product consistency and purity and understand the importance of ingredient verification for consumer safety and trust.
Don’t miss this great opportunity to deepen your understanding of the supplement landscape and stay ahead in the competitive dietary supplement market by leveraging the latest chromatography technologies!
Key learning points
<ul>
<li>Learn how standard methods are developed and how to incorporate new chromatographic techniques to maintain regulatory compliance
</li>
<li>Discover the latest innovations in chromatography and how these technological advancements can revolutionize your analysis
</li>
<li>Gain insights from industry experts to improve workflow efficiency and ensure product consistency and consumer trust
</li>
</ul>
<blockquote>
Presenter: Jinchuan Yang, PhD (Waters Corporation)
</blockquote>
<blockquote>
Dr. Jinchuan Yang is a Principal Scientist at Waters Corporation, and a subject matter expert in food and environmental applications, polymer chromatography, and LC and MS technologies. Since he joined Waters in 2002, his roles at Waters have mainly focused on application development, market development, and support for R&amp;D and training. He is an active participant in technical stakeholder groups at AOAC International and USP, serving as an expert panel member in AOAC SPIFAN and participating in review, recommendation and muti-lab testing of AOAC methods.
</blockquote>

New Food: How innovation has shaped dietary supplement testing for quality and compliance

How innovation has shaped dietary supplement testing for quality and compliance

The evolving landscape of dietary supplements, where industry experts will discuss standard methods, advanced analytical techniques, and quality control.

How innovation has shaped dietary supplement testing

In this webinar, typical LC problems are discussed - how to identify and solve them.<blockquote>Presenter: Ansuman Mahato (Product Specialist Liquid Phase Separations, Agilent Technologies)Ansuman has been a Product Specialist at Agilent Technologies for 14 years, working with the pharmaceuticals, biopharmaceuticals, food, and chemicals industries. Based in Waldbronn, Germany, the manufacturing site for Agilent’s chromatography solutions, he helps make labs more efficient and compliant and has rich experience with techniques like HPLC, UHPLC, GPC/SEC, Prep LC, and 2D-LC.Ansuman is also skilled in polymer analysis using advanced tools like viscometry and MALS. He has published many papers in international journals and is a popular speaker for his practical insights and innovative solutions.</blockquote>

Agilent Technologies: HPLC Beginner Webinar Series

Troubleshooting and Everyday Routine for the HPLC Instrument

In this webinar, typical LC problems are discussed - how to identify and solve them.

AQbD Symposium 2024 (Impact of Q14/Q2 on LC Procedures)Analytical Quality by Design (AQbD) is a science and risk-based approach to analytical procedure development that “starts with the end in mind”.The recently adopted quality guidelines Q14 and Q2(R2) leverage AQbD principles. What does that mean for the Pharma LC lab? We are joined by industry thought leaders for an engaging discussion that will encompass strategies and case studies.<p data-ccp-props="{&quot;335551550&quot;:1,&quot;335551620&quot;:1,&quot;335559683&quot;:0,&quot;335559685&quot;:0,&quot;335559731&quot;:0,&quot;335559737&quot;:0,&quot;335562764&quot;:2,&quot;335562765&quot;:0.9,&quot;335562766&quot;:4,&quot;335562767&quot;:10,&quot;335562768&quot;:4,&quot;335562769&quot;:0}" lang="EN-US">Each session will feature a panel of forward thinking leaders on the topic who will present their recent work and participate in a round table discussion.&nbsp;<h5>Learning Objectives</h5><ul><li id="isPasted">Understand opportunities and barriers related to the implementation of Q14</li><li id="isPasted">Learn about analytical development strategies for every phase of clinical development</li><li id="isPasted">Explore case studies applying Q14 to today’s analytical challenges</li><li id="isPasted">Engage with pharma industry thought leaders</li></ul><h4>Session 1: Analytical LC Method Strategies in the Q14 Era</h4><ul><li>Tuesday, December 3, 2024 | Time: 10:00am ET | 3:00pm GMT | 4:00pm CET</li></ul>With the Q14 and Q2(R2) guidelines now adopted by FDA and EMA, there is an opportunity to rethink strategies around analytical LC procedure development. Platform analytical procedures, phase-appropriate approaches, future proofing procedures for robustness, sustainability and user-friendliness are top-of-mind topics. Our panelists will discuss opportunities and barriers in the implementation of Q14.<blockquote>Presenter: Timothy Graul, Ph.D. (Director, Global CMC Advisory Office, Pfizer, Inc.)Timothy W. Graul, Ph.D.,is a Director in the CMC Advisory Office within Pfizer at the Groton, CT site. He received his B.S. Degree in Chemistry at James Madison University and then Ph.D. in Analytical Chemistry at The Florida State University. After completing studies, Tim joined Pfizer Analytical R&amp;D and supported the development of drug product formulations and drug substance synthetic routes. During his time at Pfizer, Tim has been recognized as a leader in Quality by Design for analytical procedures. He has collaborated on publications that have been at the forefront of industry in this area. Tim served as the PhRMA Deputy Topic lead on the ICH Q2/Q14 Expert Working Group and is now an Industry Expert on the Implementation Working Group for the revised Q2 Guideline and new guideline, Q14. He has been a member of industry groups including Land O’ Lakes Pharmaceutical Analysis Conference Planning Committee and AAPS Summer Scientific Forum since 2010, IQ groups such as Analytical Leadership, AQbD, Dissolution, Control Strategy Harmonization Metrics, and Worldwide Specification Harmonization; and is the co-lead of the ISPE PQLI group on Analytical Methods. Presenter: Gerald Gellermann, Ph.D. (Associate Director Scientific Office Analytics, Technical Research and Development, Novartis)Gerald currently works as Scientific Officer at Novartis Technical Research and Development (TRD) Biologics. He is member of the Novartis ICHQ12 implementation team and leads the TRD biologics QbD and Control Strategy initiative. Prior to joining Novartis, he gained professional experience in CMC and analytical development during his time at Roche and in neuroscience research and diagnostic division at Abbott/AbbVie. Gerald holds a master’s degree in biology from the University of Constance and a PhD in Molecular Biology from the University of Jena (both Germany). Gerald also represents Novartis in the industry consortia workstreams supporting ICH Q2(R2) and ICH Q14. Presenter: Andrea Gheduzzi (Principal LC Market Dev. Manager, EMEA, Waters Corporation)Andrea Gheduzzi, is a principal LC market development manager for EMEA at Waters Corporation. Andrea Gheduzzi has spent more than 30 years in the chromatography field. Across his career he had various roles, service engineer, chromatography lab manager position, and technical manager for chromatography columns.He has mainly been dealing with pharma accounts, also supporting LC method development activities using DoE/QbD approaches. In his previous role as Pharma Business Development Manager at Waters he has further expanded his expertise in AQbD and related applications.Since 2023 he became principal LC market development manager for EMEA and member of the AQbD working party at the EDQM. Moderated by: Isabelle Vu Trieu (Pharmaceutical Market Development Manager, Americas, Waters Corporation)Isabelle is a business development professional dedicated to delivering benefits to analytical scientists in the life sciences industry. With over 20 years of experience in providing chromatography and mass spectrometry solutions, Isabelle is particularly interested in helping pharmaceutical scientists achieve the data quality and integrity needed to drive decisions for drug quality, safety and efficacy. Isabelle joined Waters in 2009 and currently manages the development of the pharmaceutical market in the Americas. She holds Masters’ degrees in Chemistry from Columbia University and the University Claude Bernard in Lyon, France as well as a Chemical Engineering degree from Ecole Supérieure de Chimie Physique Electronique in Lyon, France. &nbsp;</blockquote>

Waters Corporation: AQbD Symposium 2024 Analytical Quality by Design

AQbD Symposium - Day 1 - Analytical LC Method Strategies in the Q14 Era

Our panelists will discuss opportunities and barriers in the implementation of Q14.

AQbD: Analytical LC Method Strategies in the Q14 Era

AQbD Symposium 2024 (Impact of Q14/Q2 on LC Procedures)Analytical Quality by Design (AQbD) is a science and risk-based approach to analytical procedure development that “starts with the end in mind”.The recently adopted quality guidelines Q14 and Q2(R2) leverage AQbD principles. What does that mean for the Pharma LC lab? We are joined by industry thought leaders for an engaging discussion that will encompass strategies and case studies.<p data-ccp-props="{&quot;335551550&quot;:1,&quot;335551620&quot;:1,&quot;335559683&quot;:0,&quot;335559685&quot;:0,&quot;335559731&quot;:0,&quot;335559737&quot;:0,&quot;335562764&quot;:2,&quot;335562765&quot;:0.9,&quot;335562766&quot;:4,&quot;335562767&quot;:10,&quot;335562768&quot;:4,&quot;335562769&quot;:0}" lang="EN-US">Each session will feature a panel of forward thinking leaders on the topic who will present their recent work and participate in a round table discussion.&nbsp;<h5>Learning Objectives</h5><ul><li id="isPasted">Understand opportunities and barriers related to the implementation of Q14</li><li id="isPasted">Learn about analytical development strategies for every phase of clinical development</li><li id="isPasted">Explore case studies applying Q14 to today’s analytical challenges</li><li id="isPasted">Engage with pharma industry thought leaders</li></ul><h4>Session 2: Applying Q14 to Today's Analytical Challenges</h4><ul><li>Wednesday, December 4, 2024 | Time: 10:00am ET | 3:00pm GMT | 4:00pm CET</li></ul>With the introduction of Q14 and Q2(R2), companies started working on applying these guidance to challenging analytical methods. Using appropriate software, the analytical methods are developed and validated. Our panelists will discuss some of these analytical challenges and how to overcome them by implementing Q14 and updating towards Q2(R2) in their procedures and processes.<blockquote>Presenter: James Sweatman, BSc. (Analytical Investigator, Almac Sciences)James Sweatman holds a bachelor’s degree in chemistry from the University of Liverpool, UK and a master’s degree in analytical chemistry from the university of Huddersfield, UK and is currently an Analytical Investigator at Almac Sciences in Northern Ireland. With a career in analytical chemistry spanning over 28 years in the environmental and healthcare fields, he is an expert in analytical method development for small molecules with various chromatographic and spectroscopic techniques. Since joining Almac Sciences in 2007, he has led the analytical development and validation support for many small molecule GMP manufacturing campaigns. He previously conducted a trial of the FusionQdB®softwareand is now working to implement this at Almac Sciences. Presenter: Jerome Pretre, MSc. (Analytical Technical Leader, Almac Sciences)Jerome Pretre holds a master’s degree in biochemistry from the University of Besancon, France and is currently an Analytical Technical Leader at Almac Sciences in Northern Ireland. &nbsp;With a career spanning over 20 years in analytical chemistry, he is an expert in analytical method development for small molecules and peptides with various techniques such as LC, GC, IC or KF. Since joining Almac Sciences in 2015, he has led the development and validation of several analytical purity methods for peptide drug substances. He previously established a systematic approach for the development of these new purity methods, and he is now working on implementing the Analytical Quality by Design (AQbD) approach using the Fusion QbD® software.  Presenter: Arnaud Delobel Ph.D. (R&amp;D and Innovation Director, Quality Assistance)Arnaud Delobel is the R&amp;D and Innovation Director at Quality Assistance, with expertise in analytical sciences spanning across all types of therapeutic modalities. He is a recognized expert in the 'MAB Working Party' at the European Directorate for the Quality of Medicines (EDQM) in Strasbourg. Additionally, he contributes to academic discourse through lectures at the University of Strasbourg.Arnaud graduated from ECPM Strasbourg, focusing his PhD on the mass spectrometry of proteins and peptides at the CNRS in Gif-sur-Yvette, France. He then completed a post-doctoral fellowship in Liège, Belgium, centered on proteomics. Joining Quality Assistance in 2006, Arnaud has climbed the ranks, holding various roles before assuming his current position as R&amp;D and Innovation Director in 2016.In his role, Arnaud oversees internal R&amp;D initiatives aimed at implementing cutting-edge analytical technologies. These technologies are designed to be offered by Quality Assistance to its clientele. He also guides Product Experts in identifying market needs, leveraging insights from Management, Regulatory Affairs, external experts, and comprehensive data analytics. His team works diligently to transform these insights into innovative R&amp;D services. Presenter: Ilse Hoes, Ph.D. (Manager Informatics Customer Success Team, WGS Europe, Waters Corporation)Ilse Hoes presides over a distinguished team of European consultants at Waters. The consultancy team provides unparalleled global support, specializing in the meticulous analysis and optimization of workflows. They also offer comprehensive training on the Empower Method Validation Manager, ensuring clients achieve excellence in their operations. Presenter: Arjan Timmerman (Pharma Market Development Manager EMEA, Waters Corporation)Arjan Timmerman is a market development manager at Waters working in EMEA. Prior to joining Waters in 2014 he worked for 17 years as an Analytical Scientist in the Small Molecule Pharmaceutical industry in various Chemical and Pharmaceutical late-stage development labs.Since 2023 he became QA/QC market development manager for EMEA working in a dedicated team and focuses on Pharmaceutical workflows, AQbD, Impurities, LC Chromatography and Compliance.</blockquote>

AQbD Symposium - Day 2 - Applying Q14 to Today's Analytical Challenges ​

Our panelists will discuss analytical challenges and how to overcome them by implementing Q14 and updating towards Q2(R2) in their procedures and processes.

AQbD: Applying Q14 to Today's Analytical Challenges ​

In this educational back-to-school webinar, James Grinias (University of Rowan) and Frank Steiner (Thermo Scientific) discuss strategic approaches for development, optimization and transfer of legacy and modern HPLC methods, utilizing gradient elution.Discussion topics and data on how to more easily move to core-shell column technology, how to modify gradient methods effectively, and how to work within the framework of evolving rules for method transfer such as USP 621.We also discuss using the right tool to reduce the impact of method transfer across HPLC platforms. &nbsp;Key learning objectives:<ul><li>&nbsp;Understanding gradient-based LC separations</li><li>Scalable parameters in pharmacopeia method modernization</li><li>&nbsp;Tricks and tips on method development and transfer</li></ul>If you are unable to attend, please register to ensure you will receive the link to the recording.<blockquote>Presenter: James Grinias (Associate Professor, Department of Chemistry &amp; Biochemistry, Rowan University)James P. Grinias, Associate Professor, Rowan University, teaches analytical and bioanalytical chemistry. His research focuses on UHPLC columns and instrument miniaturization. His work has been recognized with recognition from the American Chemical Society Subdivision on Chromatography and Separations Chemistry, LCGC magazine, and The Analytical Scientist magazine. Presenter: Frank Steiner (Sr. Manager of Product Applications and Scientific Advisor, Chromatography and Mass Spectrometry, Analytical Instrument, Thermo Fisher Scientific)Dr. Frank Steiner is Sr. Manager of Product Applications and Scientific Advisor in the HPLC organization of Thermo Fisher Scientific and coordinates scientific collaborations with external partners to advance UHPLC technologies and applications.</blockquote>

Thermo Fisher Scientific: The real impact of HPLC and column parameters

The real impact of HPLC and column parameters

We will discuss strategic approaches for development, optimization and transfer of legacy and modern HPLC methods, utilizing gradient elution.

System troubleshooting can be a difficult process with a major impact on productivity. Getting a system diagnosed and repaired quickly can improve system usage and allow for work to be completed on-time with minimal out of spec results. In order to perform troubleshooting effectively, certain protocols need to be in place which can better facilitate those activities.In this webinar, a simple and easy to follow protocol for troubleshooting LC systems will be proposed. Included will be how to set up a system performance benchmark, which will be used as a part of routine qualification checks to ensure system performance is not declining. As a part of this webinar several examples of failed chromatography and poorly performing systems will be examined and the issues therein troubleshooted.Who Should Attend:<ul><li>People who routinely run liquid chromatography systems and need to potentially troubleshoot failed chromatograms.</li><li>Lab managers who want to better understand how to troubleshoot LC systems to reduce system downtime and improve productivity</li></ul>Learning objectives<ul><li>Implement a system monitoring protocol</li><li>Use a system monitoring protocol to troubleshoot a failed injection</li><li>Quickly identify failure causes and troubleshoot minor system failures</li></ul><blockquote>Presenter: Kenneth Berthelette (Senior Scientist, Waters Corporation Chemistry and Consumables Group)Ken earned his Bachelor's of Science degree in Professional Chemistry from Bridgewater State University, Bridgewater Massachusetts, in 2010. He has been with Waters Corporation since March 2012 and has worked on several product launches including CORTECS columns, UPC2 Torus and Trefoil columns, and the Atlantis Premier BEH Z-HILIC column. He changed job functions in 2021 and now supports Chemistry Aftermarket initiatives. His focus on applications and customer education has led to several seminars and webinars on topics including method development, method modernization and transfer, HILIC separation techniques, and LC system troubleshooting.</blockquote>

Waters: Chromatographic System Troubleshooting: A Systematic Approach

Chromatographic System Troubleshooting: A Systematic Approach

In this webinar, a simple and easy to follow protocol for troubleshooting LC systems will be proposed.

Chromatographic System Troubleshooting

Lean manufacturing – streamlining manufacturing processes to optimize productivity and reduce waste – has the potential to transform biopharmaceutical production.This webinar will explore how eliminating non-value-adding steps within manufacturing can boost consistency and reliability in production, which in turn enhances facility efficiency.Our expert speaker will focus on the role of pre-packed chromatography columns in lean manufacturing, discussing how to incorporate them into biopharmaceutical production to enhance operation efficiency, reduce costs and support more reliable and productive outcomes.&nbsp;Attend this webinar to:<ul style="list-style-type:disc;"><li style="tab-stops:list 36.0pt;">Learn about the benefits of adopting a lean manufacturing approach across process development, clinical manufacturing and commercial production</li><li style="tab-stops:list 36.0pt;">Explore the strategic advantages of incorporating pre-packed chromatography columns into biopharmaceutical manufacturing</li><li style="tab-stops:list 36.0pt;">Discover how to ensure consistent process outcomes and better compliance with regulatory standards</li></ul><blockquote>Presenter: Adam Nelson (Chromatography Product Manager at Repligen Corporation)Adam Nelson has 14 years of experience in pre-packed chromatography column manufacturing and product development. In his current role as product manager for OPUS large scale pre-packed chromatography columns, he is responsible for new product development, product line extensions and product innovation. In prior roles, Adam developed packing protocols that enable OPUS pre-packed chromatography column packing technology. Additionally, Adam led the team responsible for materials management, process control, and process development for OPUS large scale chromatography column manufacturing. He has a passion for customer service and leveraging his technical expertise to deliver an optimal customer experience. Adam holds a bachelor's in chemical engineering from the University of Massachusetts Amherst and a graduate's certificate in innovation management from Northeastern University.</blockquote>&nbsp;

Technology Networks: Enhancing Efficiency and Quality in Biopharmaceutical Manufacturing

Enhancing Efficiency and Quality in Biopharmaceutical Manufacturing

Eliminating non-value-adding steps within manufacturing can boost consistency and reliability in production.

Efficiency and Quality in Biopharmaceutical Manufacturing

Join us for an exclusive webinar where we’ll provide an overview of the recently launched Agilent Infinity III LC Series.&nbsp;The session will include a demonstration of the new Agilent InfinityLab Assist technology introduced with the Infinity III LC Series, providing more automation, fewer errors, and increased system uptime in your daily HPLC routine.Don’t miss this chance to stay at the forefront of HPLC technology and engage directly with our experts.Key learning objectives<ul style="list-style-type:disc;"><li style="tab-stops:list 36.0pt;">How to enhance assistance with routines:&nbsp;Explore new features designed to simplify recurring tasks and maximize instrument uptime.</li><li style="tab-stops:list 36.0pt;">How to achieve confidence in results: Discover Agilent’s comprehensive solutions and learn how to leverage 50 years of LC expertise to your advantage.</li><li style="tab-stops:list 36.0pt;">How to protect your investment:&nbsp;Understand how to increase flexibility for maintaining and upgrading your instruments according to your needs and budget.</li><li style="tab-stops:list 36.0pt;">How to make your lab sustainable:&nbsp;Gain insights into reducing your lab’s environmental footprint by reducing water, solvent, and energy consumption.</li></ul>Who should attend?Laboratory managers and LC users in pharmaceuticals, biopharmaceuticals, energy and chemicals, food industry, environmental sciences, and academia who utilize high-performance liquid chromatography (HPLC) and ultra-high-performance liquid chromatography (UHPLC) instruments.Certificate of attendance All webinar participants can request a certificate of attendance, including a learning outcomes summary, for continuing education purposes.<blockquote>Presenter: Jens Meixner, PhD (Product Manager, Agilent Technologies, Inc.)Jens Meixner is a product manager at Agilent Technologies, where he oversees the analytical core HPLC and CE-MS portfolios. He began his career in 2014 as a PhD student at Aalen University and the University of Tuebingen, where he studied analytical chemistry. During his PhD he focused on the development of multidimensional separation techniques like CExCE and LCxCE coupled with high-end MS detection for the characterization of therapeutic drugs.Presenter:&nbsp;Florian Rieck, PhD (Scientist Application Development, Agilent Technologies, Inc.)Florian Rieck holds a master's degree in food chemistry and a PhD in analytical chemistry from the Karlsruhe Institute of Technology (KIT) in Germany. Florian joined Agilent Technologies in 2015 as an applications chemist for liquid chromatography. An author of numerous application notes covering different analytes and detection techniques, he has also been responsible for a number of customer collaborations and demonstrations of the Agilent InfinityLab LC portfolio.Moderator: Olivia Long (Editorial Team, SelectScience)</blockquote>

SelectScience: HPLC Made for Tomorrow: Introducing the new Agilent Infinity III LC Series

HPLC Made for Tomorrow: Introducing the new Agilent Infinity III LC Series

Join us for an exclusive webinar where we’ll provide an overview of the recently launched Agilent Infinity III LC Series.

Introducing the new Agilent Infinity III LC Series

XDCs, such as antibody-drug conjugates (ADC), antibody oligo conjugates (AOC), ribonucleoproteins (RNP), and conjugate vaccines are growing rapidly due to advances in their component technology and their promise of higher efficacy compared to traditional therapies.&nbsp;However, these complex hybrids are more heterogeneous than traditional biologics, posing additional analytical challenges. Quality attributes such as composition, aggregation, purity, and conformation can all affect the efficacy and toxicity of the product.In this online webinar, we will demonstrate the value of light scattering in XDC development and its potential application in quality control (QC) as part of a release assay. We will explore how light scattering provides reproducible and comprehensive quality attribute data in a single platform and provides savings on time and resources for the development of complex XDC drugs.&nbsp;

Waters Corporation: Analysis of PCVs, AOCs, ADCs, and RNPs with Light Scattering

Analysis of PCVs, AOCs, ADCs, and RNPs with Light Scattering

In this online webinar, we will demonstrate the value of light scattering in XDC development and its potential application in QC as part of a release assay

Protein-based therapeutics such as monoclonal antibodies are widely developed and used by the biopharmaceutical industry; in 2023, mAb-based therapeutics represented over half of the Top Ten best-selling drugs of the year, globally, and are one of the fastest growing modalities. However, these large molecules pose unique challenges in relation to sample preparation and analytical characterisation, making robust and fit-for-purpose methods essential to achieve high quality results.This December, we invite you to connect with fellow scientists to learn more about the latest innovations in Protein analysis at our ProteinConnect Virtual Forum. Through a selection of complementary presentations and resources, our specialists will share insights into the present and future of protein characterisation, including:<ul><li>How workflow solutions can address mAb CQA analysis</li><li>Hints and tips for optimising protein bioseparations</li><li>The latest tools and technologies to enhance your protein analysis</li><li>Novel approaches to support online analysis and production of mAbs</li><li>A look to the future – emerging trends in Biopharma analysis</li><li>And much more! agilent ms</li></ul><h5>Agenda 10 December 2024</h5>10:30 a.m. Approaching The Analysis Of mAbsAnd Protein-based Therapeutics: An Introduction11:15 a.m. Optimizing Protein Therapeutic Analysis with Agilent Columns: Innovations And Applications12:15 p.m. Online LC: A Powerful Tool For Process Monitoring During The Production Of mAbs13:00 p.m. ProteoAnalyzer: Analysis of NIST mAbReference Material By Parallel SDS-Capillary Electrophoresis

Agilent Technologies: ProteinConnect: Linking Science, Innovation and Therapeutics

ProteinConnect: Linking Science, Innovation and Therapeutics (Day 1)

We invite you to connect with fellow scientists to learn more about the latest innovations in Protein analysis at our ProteinConnect Virtual Forum.

LCMS webinars focusing on HPLC - page 19

ProteinConnect: Linking Science, Innovation and Therapeutics (Day 1)

Analysis of PCVs, AOCs, ADCs, and RNPs with Light Scattering

HPLC Made for Tomorrow: Introducing the new Agilent Infinity III LC Series

Enhancing Efficiency and Quality in Biopharmaceutical Manufacturing

Chromatographic System Troubleshooting: A Systematic Approach

AQbD Symposium - Day 2 - Applying Q14 to Today's Analytical Challenges

The real impact of HPLC and column parameters

AQbD Symposium - Day 1 - Analytical LC Method Strategies in the Q14 Era

How innovation has shaped dietary supplement testing for quality and compliance

Troubleshooting and Everyday Routine for the HPLC Instrument

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LCMS webinars focusing on HPLC - page 19

ProteinConnect: Linking Science, Innovation and Therapeutics (Day 1)

Analysis of PCVs, AOCs, ADCs, and RNPs with Light Scattering

HPLC Made for Tomorrow: Introducing the new Agilent Infinity III LC Series

Enhancing Efficiency and Quality in Biopharmaceutical Manufacturing

Chromatographic System Troubleshooting: A Systematic Approach

AQbD Symposium - Day 2 - Applying Q14 to Today's Analytical Challenges ​

The real impact of HPLC and column parameters

AQbD Symposium - Day 1 - Analytical LC Method Strategies in the Q14 Era

How innovation has shaped dietary supplement testing for quality and compliance

Troubleshooting and Everyday Routine for the HPLC Instrument

Related content

News from LabRulezLCMS Library - Week 27, 2026

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AQbD Symposium - Day 2 - Applying Q14 to Today's Analytical Challenges