Professor Michaela Rumlová, head of a research group of the National Institute of Virology and Bacteriology at UCT Prague (and Vice-Rector for doctoral studies), holder of a licensed patent &nbsp;for antiretroviral testing assays, and recipient of the Czech Science Foundation’s President’s Award for HIV research, has also become the principal investigator of the bilateral INTER-EXCELLENCE II project “Cellular factors regulating the export and packaging of unspliced ​​retroviral RNA”, starting this year. In this interview, she explains how retroviruses such as HIV “hack” the cell in order to export their own genome from the nucleus into the cytoplasm, and then to the site where it is specifically packaged into nascent viral particles, a key step in viral replication. Her team is collaborating with the NIH (National Institutes of Health) and Rockefeller University to elucidate mechanisms that may help reveal new vulnerable points in the retrovirus replication cycle while simultaneously clarifying related cellular processes.<h5>Could you describe your project and its main objectives?</h5>Retroviruses, such as HIV, are viruses that carry genetic information in the form of RNA; however, after entering a cell, they are able to reverse transcribe it into DNA and integrate it into the host genome. In order to replicate, they must transport their genetic information—in the form of unspliced RNA—from the nucleus into the cytosol. There, it serves both as a template for the production of viral proteins and as the genome of new viral particles. This is highly unusual, as the cell very carefully controls which RNA molecules are allowed to leave the nucleus. Normal protein-coding cellular RNAs, known as messenger RNAs (mRNAs), must undergo splicing—a processing step in which certain segments are removed—before being exported. When this process fails, mRNA that retains these segments is held back in the nucleus as part of quality control and subsequently degraded. Retroviruses have managed to outwit this cellular control machinery, allowing their unspliced RNA to pass into the cytoplasm.Our project focuses on identifying the cellular proteins that assist retroviruses in this step and on the mechanism by which protein complexes bound to viral RNA are remodelled during RNA transport. The goal is to understand how the virus uses cellular mechanisms to its advantage and how this pathway is linked to the subsequent fate of the RNA, i.e. to its translation and packaging into new viral particles.<h5>Why is this export of unspliced ​​RNA so important for retroviral replication?</h5>Because this RNA has a dual role for the virus. On the one hand, it serves as a template for synthesizing viral proteins in the cytoplasm; on the other, it represents the genetic material itself, which must be packaged into new viral particles. The virus must package its RNA genome in its unspliced form, as only this RNA contains essential sequences without which further viral replication would be impossible.Simply put: the virus needs to get a complete copy of its genetic instructions out of the nucleus. Once the viral RNA is in the cytoplasm, it can be used as a template for synthesizing viral proteins; and a portion of these RNA molecules is then selected and packaged into a new particle as a genome. We are interested in how the virus forces the cell to allow viral RNA that is, from the cell’s perspective, “unfinished,” that is unspliced, to leave the nucleus. We are also investigating how the cell and the virus together - albeit unintentionally from the cell’s perspective - determine which RNA molecule will be translated into proteins and which will be packaged as the genome. This is one of the key, yet still not fully understood, steps of retroviral replication.<h5>What do you think it was about your project that impressed the Ministry of Education, Youth and Sports’ jury the most?</h5>I believe the decisive factor was a combination of several elements. First, the reviewers were primarily interested in the scientific problem itself. We are investigating a mechanism that is essential for retroviruses, but still not sufficiently understood, which may also have broader implications for the regulation of cellular processes. In addition, the quality of the team, our strong collaboration with the NIH and Rockefeller University, and the use of modern methods that will allow us to track viral RNA in great detail also played a role. It was also important that the project has clear objectives and a realistic plan.<h5>How will this research contribute to the development of new treatments for retroviral infections?</h5>It is important to say that this is primarily basic research. Rather than developing a new drug directly, we are trying to understand, at the molecular level, the step that the virus cannot do without. Only when we fully understand the mechanism of these cellular processes and identify the essential factors for the export of unspliced ​​RNA and how they function, will we be able to better determine which interactions or mechanisms represent potentially vulnerable points. Similar mechanisms associated with the export of unspliced ​​mRNA also appear in some diseases, such as cancer, so I &nbsp;see the benefit of our research mainly in that it will refine our understanding of these pathological processes in the cell, and retroviruses help us do so. Therefore, our work on retroviruses not only shows us how the virus works, but also how the cell itself works.<h5>What responsibilities does your UCT Prague team have? How does the collaboration with your American partners work?</h5>At UCT Prague, we are conducting the project’s primary experimental work. Our research involves cell cultures, viral models, and various biochemical techniques ranging from immunochemistry to microscopy that allow us to track viral RNA and identify its cellular partners. In collaboration with colleagues from NIH, Frederick, we are developing single-particle tracking methods to observe viral RNA movement in living cells across both space and time. Furthermore, we are working with colleagues from Rockefeller University to employ iCLIP methods for the precise mapping of protein-binding sites on RNA. Both collaborations are valuable to us, because they are not just about data sharing, but also about the transfer of expertise and detailed know-how.<h5>Who else is collaborating on the project?</h5>At UCT Prague, we collaborate closely with the Department of Biotechnology and the Department of Biochemistry and Microbiology. Externally, we work with colleagues from the Institute of Organic Chemistry and Biochemistry of the CAS (IOCB Prague) and the Central European Institute of Technology in Brno (CEITEC). Our research naturally necessitates the integration of diverse fields of expertise; it requires combining virology, cell biology, and biochemistry with advanced imaging and modern approaches to study RNA-protein interactions. This is exactly what makes our project capable of addressing complex questions that a single laboratory cannot tackle on its own.<h5>Are there plans to continue the project? Have you brought on new collaborators, and are you looking to welcome new colleagues to the team?</h5>Yes, definitely. In basic research, it often happens that one answered question opens up several more. High-quality, properly interpreted data provide a foundation you can build on — and that is what moves scientists forward. It is already clear that the topic of retroviral RNA export and packaging has broader implications and may reveal more general principles of how RNA functions within the cell. We also hope the project will be attractive to students, as it connects classical molecular biology, biochemistry, and virology with cutting-edge biotechnological methods. Students are already involved in the project as part of their bachelor’s and master’s theses.<h5>What was the personal turning point that motivated you to pursue a long-term investigation into retroviral particles?</h5>I first encountered retroviruses in the early 1990s during my diploma thesis, when I was working at IOCB Prague on HIV-1 protease inhibitors. At that time, AIDS could not yet be effectively treated, and retrovirus research had a completely different dimension. From the very beginning, I was fascinated by how such a relatively small and simple virus can outsmart evolutionarily highly optimized cellular processes and turn them to its own advantage. And that has never stopped fascinating me. From a scientific point of view, retroviruses are remarkable; just consider one of their enzymes, reverse transcriptase, without which no diagnostic or research laboratory in the world could function today. Without it, it would be impossible to diagnose RNA viruses like SARS-CoV-2, influenza, or HIV using RT-PCR tests, or, for example to produce recombinant human proteins in bacterial cells.

Tracking unspliced RNA: Professor Rumlová studies how retroviruses “hack” cells

News from LabRulezLCMS Library - Week 20, 2026

🎤Lindsay Repka&nbsp;Lindsay Repka is a chemistry professor at Middlebury College whose research explores photoredox chemistry, catalyst stability, and visible-light-driven transformations. Her lab emphasizes both mechanistic insight and hands-on student training in advanced analytical instrumentation.In this episode of Concentrating on Chromatography, we sit down with Lindsay Repka to discuss how LC-MS and GC-MS transformed her lab’s approach to photoredox chemistry.What began as a project to develop a visible-light photocrosslinking handle unexpectedly led to a major discovery: the solvent (DMF) was reacting with the photocatalyst itself. Using high-resolution LC-MS, Lindsay’s team observed multiple solvent adducts forming — sometimes with complete catalyst consumption. That discovery reshaped their research direction.This episode is a rare deep dive into both LC-MS and GC-MS within the same research project, showing how chromatography-driven insight can turn unexpected degradation into productive new reactivity.If you work in:<ul><li>Photoredox chemistry</li><li>Reaction optimization</li><li>Mass spectrometry method development</li><li>Catalyst screening</li><li>Academic synthetic chemistry</li></ul>…this conversation will resonate.<h4>Key Topics Covered</h4><ul><li>Photocatalyst stability in DMF, DCE, and MeCN</li><li>Demethylation under mild visible-light conditions</li><li>High-resolution Q-TOF LC-MS quantitation</li><li>Internal standard methodology in GC-MS</li><li>Signal-to-noise improvement using extracted ion chromatograms</li><li>Reaction reproducibility and quality control strategy</li></ul><h3>Video Transcription</h3>In this interview, Lindsay Repka discusses the role of photoredox chemistry in method development, photocrosslinking, solvent activation, catalyst stability, and the use of LC-MS and GC-MS to understand reaction pathways.<h4>What is photoredox chemistry?</h4>Photoredox chemistry uses light to activate a photocatalyst, which can either donate or accept an electron from a substrate. This generates radical species that can undergo new types of reactivity.Repka explains that one of the key advantages of photoredox chemistry is its ability to operate under mild conditions, often using visible light. This makes it attractive for sensitive molecules and biological systems, including potential applications in drug release, live-cell chemistry, and drug development.<h4>Photocrosslinking and drug-target interactions</h4>Repka’s group initially aimed to develop visible-light photocrosslinking methods to study drug interactions with biological targets. Photocrosslinking can covalently link a drug to its target, making it easier to identify the biological target of a compound.Traditional photocrosslinking often uses UV light, but visible light offers milder and more biologically compatible conditions.<h4>From photocrosslinking to solvent activation</h4>The project shifted direction when the desired photocrosslinking reactions gave very low yields. Instead of reacting with amino acids as intended, the radical intermediate mainly reacted with itself.The team explored different solvents to stabilize the radical, but found that some solvents, such as DMF, were not inert. Instead, the solvent reacted with the photocatalyst or radical species, revealing unexpected solvent activation chemistry.<h4>Why LC-MS was critical</h4>LC-MS became essential because the reaction mixtures were too complex and low-yielding for NMR or GC-MS. High-resolution LC-MS allowed the team to detect minor products, identify photocatalyst modifications, and observe solvent addition products with high confidence.Repka emphasizes that LC-MS was both a qualitative and quantitative tool. It was used quantitatively to measure how much photocatalyst remained after light exposure, and qualitatively to identify degradation products and new reactivity pathways.<h4>Photocatalyst stability workflow</h4>The team developed a systematic workflow to evaluate photocatalyst stability under light exposure. Because catalyst degradation was time-sensitive, reproducibility required strict control of experimental conditions.Key steps included using a glove box, commercial deoxygenated solvents, accurate weighing on a microbalance, controlled light exposure in a photoreactor, and temperature control with a chiller. These precautions helped reduce day-to-day variability and enabled reproducible measurements of catalyst remaining.<h4>Quantifying catalyst remaining by LC-MS</h4>Calibration curves were prepared using pure photocatalyst solutions and LC-MS ion areas. The team worked in the nanomolar range to maintain linearity.Instead of relying only on total ion chromatograms, they used extracted ion chromatograms (EICs) to improve signal-to-noise for the photocatalyst signal. Baseline subtraction was also applied to reduce noise and improve quantitation at low concentrations.Calibration curves and quality control samples were prepared and measured on the same day as the reaction samples, because LC-MS signal intensity can vary significantly from day to day.<h4>Why GC-MS was used later</h4>For demethylation studies, the group moved to GC-MS because the substrates and products were small and volatile enough for gas chromatography. GC-MS was better suited for rapidly screening many reaction conditions, including different photocatalysts, solvents, and oxidants.Instead of generating full calibration curves for every experiment, the team used an internal standard to streamline analysis and determine percent conversion based on relative response factors.<h4>Practical lessons from the project</h4>Repka highlights the importance of careful sample preparation, especially when working with very small quantities of photocatalysts. A high-quality microbalance, stable granite platform, antistatic tools, and aluminum weigh boats improved weighing accuracy and reproducibility.She also argues that mass spectrometry should be a standard part of photoredox method development because it can reveal minor products, support reaction optimization, and provide insights that may be difficult or impossible to obtain by NMR alone.<h4>Final perspective</h4>Beyond the chemistry, Repka emphasizes the importance of student training and mentoring. For her, research is not only about discovering new reactivity but also about giving students practical experience, helping them develop independence, and preparing them for future scientific careers.<blockquote>This text has been automatically transcribed from a video presentation using AI technology. It may contain inaccuracies and is not guaranteed to be 100% correct.</blockquote><blockquote><h4>Concentrating on Chromatography Podcast</h4>Dive into the frontiers of chromatography, mass spectrometry, and sample preparation with host David Oliva. Each episode features candid conversations with leading researchers, industry innovators, and passionate scientists who are shaping the future of analytical chemistry. From decoding PFAS detection challenges to exploring the latest in AI-assisted liquid chromatography, this show uncovers practical workflows, sustainability breakthroughs, and the real-world impact of separation science. Whether you’re a chromatographer, lab professional, or researcher you'll discover inspiring content!You can find Concentrating on Chromatography Podcast in podcast apps:<ul><li><a target="_blank" rel="noopener noreferrer" href="https://open.spotify.com/show/1wZg67rCjoGju4yhqxI3tL">Spotify</a></li><li><a target="_blank" rel="noopener noreferrer" href="https://podcasts.apple.com/tz/podcast/concentrating-on-chromatography/id1788429185">Apple podcast</a></li><li><a target="_blank" rel="noopener noreferrer" href="https://podscan.fm/podcasts/concentrating-on-chromatography">podscan</a></li></ul>and on <a target="_blank" rel="noopener noreferrer" href="https://www.youtube.com/@ChromatographyTalk/videos">YouTube channel</a></blockquote>

Using LC-MS & GC-MS to Decode Photoredox Catalyst Stability

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<h4>European Free Educational Webinar Series</h4>
Let&#39;s go back to the basics of Chromatography, Mass Spectrometry, Data Analysis and Trouble-shooting: understanding the importance of the basics will enable you to be more effective in your daily laboratory life.
<h4>EPISODE 6</h4>
The importance of Compliance
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<li>Data Integrity Matters - A short Overview</li>
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Prezenter: Arjan Timmerman (Pharma Market Development Manager EMEA, Waters Corporation)
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Arjan Timmerman is a market development manager at Waters working in EMEA. Prior to joining Waters in 2014 he worked for 17 years as an Analytical Scientist in the Small Molecule Pharmaceutical industry in various Chemical and Pharmaceutical late-stage development labs.
</blockquote>
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Since 2023 he became QA/QC market development manager for EMEA working in a dedicated team and focuses on Pharmaceutical workflows, AQbD, Impurities, LC Chromatography and Compliance.
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Back to Basics - Sample Preparation: Theory and Overview
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Back to Basics - Theory of Liquid Chromatography
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Back to Basics - The importance of Compliance
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Waters Corporation: Back to Bacisc Free Educational Webinar Serries

The importance of Compliance

Why data integrity matters and what is important in your lab environment.

In this session, discover how Wiley’s KnowItAll saves hours of GC-MS manual analysis time by combining fast, flexible deconvolution with an integrated reference database search into a single, automated workflow to identify components.<h4>We will demonstrate through a series of live examples:</h4><ul><li>KnowItAll’s various methods for GC-MS analysis and how they can impact your workflow.</li><li>Professors and students engaged in chemistry and mass spectrometry courses or lab work.</li></ul>About KnowItAll: KnowItAll combines all the tools and spectral databases you need for effective analysis into a single, easy-to-use interface to make your lab its most efficient. Compatible with over 130 instrument formats, it streamlines your entire workflow regardless of how many techniques and instruments you use. Learn more at sciencesolutions.wiley.com<blockquote>Presenter: Dr. Leanna Ergin (Associate Editor, Wiley Science Solutions)Dr. Leanna Ergin is an Associate Editor for Wiley Science Solutions, for which she helps create tools for spectroscopy, structural, and scientific standard reference data and helps create innovative software applications. Leanna completed her PhD in Clinical-Bioanalytical Chemistry from Cleveland State University, where she developed a protocol for processing hyperspectral image data that distinguishes the spectra, removes degeneracies caused by symmetry, and is generalized to work with any number of wavelength dimensions and spectra. The results are mapped to the full RGB colorspace of modern computer displays. Leanna joined Wiley in 2022.Presenter:&nbsp;Dr. Michelle D’Souza (Product Manager, Software Products, Wiley Science Solutions)Dr. D’Souza has over 15 years of experience in the management and development of spectroscopy software, including her current position as product manager for software products at Wiley Science Solutions and prior to that at Bio-Rad Laboratories, Inc. She also worked many years in the pharmaceutical industry after obtaining a Ph.D. in chemistry from Northern Illinois University.</blockquote>

The Analytical Scientist: Introduction to KnowItAll for Mass Spectrometry

Introduction to KnowItAll for Mass Spectrometry

How Wiley’s KnowItAll saves hours of GC-MS manual analysis time.

This webinar will guide you through optimizing your Q-TOF for superior data quality by selecting the most suitable tune mode for your samples.We will delve into various MS acquisition modes, including MS only, All Ions (QRAI), Auto MS/MS (directed MS/MS; targeted MS/MS), and PRM mode.Learn how to fine-tune method settings to enhance your data quality and achieve the best analytical results. Whether you’re a seasoned user or new to QTOF, this session will provide valuable insights to elevate your mass spectrometry.<blockquote>Presenter: &nbsp;Stefan Schaebler (Application Specialist LC/MS, Agilent Technologies)Stefan completed his PhD in Biology, specializing in LC-MS-based lipidomics and metabolomics. Following his doctoral studies, he joined Agilent Technologies as an LC/MS Application Specialist. In this role, Stefan focuses on small molecule applications using LC-QTOF, with a strong emphasis on software tools and data interpretation.</blockquote>

Agilent Technologies: MassHunter Masterclass Months

MassHunter Acquisition Q-TOF Part II – Advance Settings

Join us for an in-depth exploration of advanced settings in MassHunter Acquisition for Q-TOF systems.

This webinar will guide you through optimizing your Q-TOF for superior data quality by selecting the most suitable tune mode for your samples.You will learn about the different optimisation workflows available within MassHunter Acquisition, including how to optimise Multiple Reaction Monitoring (MRM) methods for new compounds. We will also review key tools for creating and editing worklists, and discuss the benefits of Intelligent Reflex tools, demonstrating how they can be effectively implemented in your workflows.This webinar is designed to enhance your understanding and application of MassHunter Acquisition, ensuring you can achieve optimal performance in your analytical processes.<blockquote>Presenter: Day Powell (Field Application Specialist, Agilent Technologies)Day has worked for Agilent as an Environmental Application Specialist for over a year, before this he worked for United Utilities (UU) as a Technical Specialist responsible for developing new methodologies for both LC/MS and GC/MS applications. Day has worked with PFAS for several years and helped UU to UKAS accredit a novel method for 47 PFAS in drinking water using direct injection to address the latest DWI regulations. Presenter: Jonathan Spencer (Application Specialist, Agilent Technologies)Jonathan studied BSc Natural Sciences at the University of Nottingham and later went on to Sheffield Hallam University to study a MSc in Forensic Science.Following graduation, he spent 2 years as a Confirmatory Analyst in an anti-doping and forensic toxicology lab, before joining Agilent as an LC/MS Applications Scientist. At Agilent, he focuses on small molecule applications using LC/TQ and LC/Q-TOF in fields of like food, forensic and environmental analysis.</blockquote>

MassHunter Acquisition for LC/TQ and MRM Method Optimisation

This session will provide a comprehensive overview of the various aspects of MassHunter Acquisition for Liquid Chromatography/Triple Quadrupole (LC/TQ) systems.

We’ll explore how outlier setup can help you focus on samples and compounds that require your attention, ensuring accurate and reliable results.Additionally, you’ll learn how viewing compounds and calibration at a glance can expedite data review, making the process faster and more intuitive.Finally, we’ll demonstrate how easily you can summarize your results in comprehensive reports or export them to Excel, streamlining your workflow and enhancing productivity.Don’t miss this opportunity to enhance your data analysis skills with MassHunter Quant!<blockquote>Presenter: Peter Kornas (LC/MS Applikationsspezialist, Agilent Technologies)Peter Kornas has more than 10 years of experience in the development and validation of analytical methods for the determination of pesticides, mycotoxins and contaminants using LC/MS/MS. Before joining Agilent as an LC/MS application specialist in 2018, he worked as an analytical chemist in the food industry. During this time, he was responsible for the development and validation of analytical methods and worked in close coordination with Regulatory Affairs to ensure compliance with the legal requirements of the LC/MS method portfolio.</blockquote>

MassHunter Quant – A Fast and Easy Way from Data to Results

Join us and discover how quickly a quantitation method can be set up, enabling efficient data processing.

This is the second in our three-part Ask Me Anything webinar series on one of the most frequently asked about topics - processing data. The Components tab in the Processing Method displays many fields of information, giving you options about peak identification, calibration, reference peaks, and fields used in conjunction with custom fields.In Part 2 of this series, we will discuss:<ul><li>Peak identification</li><li>Calibration options including external and internal calibration</li><li>Reference Peaks for peak identification and calibration</li><li>Impurity fields</li><li>CCompRef1, 2, 3, and CConst1-7</li><li>Fields related to chemical structures</li></ul>Please join us for a lively discussion as we explore this topic. As always, please bring your questions for this exclusive opportunity to ask our experts anything about Empower Software.<blockquote>Presenter: Neil Lander (Principal Product Manager, Informatics, Waters Corporation)Neil has worked for Waters Corporation since 1994 and has held a variety of positions including Technical Support Specialist, Technical Trainer, Manager Customer Education and currently, Principal Marketing Manager focusing on Informatics solutions. He provides internal support for Empower Chromatography Data System and is focused on developing laboratory software solutions that help organizations achieve their scientific and operational objectives. Prior to joining Waters, he was an analytical chemist where he gained experience in both the cosmetics and specialty chemical industries.</blockquote>

Waters Corporation: Empower Tips: Ask Me Anything About Processing Data - Part 2

Empower Tips: Ask Me Anything About Processing Data - Part 2

This is the second in our three-part Ask Me Anything webinar series on one of the most frequently asked about topics - processing data.

Empower Tips: Ask Me Anything About Processing Data

Software can help streamline critical processes in therapeutic analysis, enhancing efficiency, ensuring compliance and maintaining product quality.This webinar explores how to implement digitization to accelerate decision-making, meet regulatory demands and eliminate bottlenecks in biotherapeutic development, helping to drive new therapeutics into the clinic. Attend this webinar to:<ul><li>Explore how analytical software can streamline and automate critical processes in therapeutic development</li><li>Understand how digital solutions can overcome bottlenecks in analytical testing</li><li>Discover best practices and strategies for harnessing the power of software</li></ul><blockquote>Presenter: Makesh Karpagavinayagam (Sr. Manager, Product Manager, at Thermo Fisher Scientific)Makesh Karpagavinayagam serves as the Senior Manager of Product Management at ThermoFisher Scientific, overseeing Rapid Microbial Identification and Software products that ensure the quality, safety and potency of pharmaceutical products. With over a decade of experience, Makesh has led product management teams, launched customer-centric products and collaborated with R&amp;D groups to provide high-quality genetic analysis solutions for applications in biopharma, forensics and academia.</blockquote>

Technology Networks: Streamlining Data for Superior Therapeutic Manufacturing

Streamlining Data for Superior Therapeutic Manufacturing

Explore how to implement digitization to accelerate decision-making, meet regulatory demands and eliminate bottlenecks in biotherapeutic development.

Automated lab workflows often require many interactions with pre-existing in-house and commercial software systems. This webinar uses the real example of building a purification factory, to give some insights on how this can be done with Mestrelab tools in conjunction with in-house and commercial software.<div data-aos-delay="0" data-aos-duration="1000"><div>Join us for an insightful webinar where we’ll demonstrate how to integrate Mestrelab’s tools into a complex IT ecosystem to automate lab workflows. Using a real-world example, we'll show you how to build a purification factory that seamlessly connects in-house and commercial software systems.<p data-ccp-props="{&quot;335551550&quot;:1,&quot;335551620&quot;:1,&quot;335559683&quot;:0,&quot;335559685&quot;:0,&quot;335559731&quot;:0,&quot;335559737&quot;:0,&quot;335562764&quot;:2,&quot;335562765&quot;:1,&quot;335562766&quot;:4,&quot;335562767&quot;:10,&quot;335562768&quot;:4,&quot;335562769&quot;:0}">Who Should Attend?​<ul style="font-weight:400;"><li data-charcodes="8226" data-font="Arial,Sans-Serif" data-buautonum="8" data-aria-posinset="1" data-aria-level="1">Lab Managers and Scientists looking to automate and enhance lab workflows​</li><li data-charcodes="8226" data-font="Arial,Sans-Serif" data-buautonum="8" data-aria-posinset="2" data-aria-level="1">IT and Automation Specialists in life sciences or chemical industries seeking integration solutions​</li><li data-charcodes="8226" data-font="Arial,Sans-Serif" data-buautonum="8" data-aria-posinset="3" data-aria-level="1">Anyone interested in embedding Mestrelab tools into a powerful, scalable IT infrastructure</li></ul><blockquote>Presenter: John Hollerton (Senior Scientific Director, Mestrelab Research)John has over 40 years of experience in the scientific and informatics fields at GSK and its predecessor companies. He has managed various analytical functions, including NMR, X-ray crystallography, mass spectrometry, physical chemistry, and chromatography, and has expertise in chemical structure representation and registration. In informatics, he led projects to create a global scientific data management system and wrote software for instrument control, data transformation, and analysis. His team developed software to automate LCMS purification factories in the US and UK.&nbsp;</blockquote></div></div>

Mestrelab: Building a Purification Factory: Embedding Mestrelab Tools into a Rich IT Infrastructure

Building a Purification Factory: Embedding Mestrelab Tools into a Rich IT Infrastructure

We’ll demonstrate how to integrate Mestrelab’s tools into a complex IT ecosystem to automate lab workflows.

Embedding Mestrelab Tools into a Rich IT Infrastructure

<div><div>Therapeutic synthetic oligonucleotides represent unique analytical challenges in late-stage development. To meet the stringent regulatory requirements, highly specific and sensitive methods such as liquid chromatography-mass spectrometry (LC/MS) must be used. However, neither chromatography nor mass spectrometry data systems have the capabilities, both in function and in data integrity, for the typical QC analyst end user to run a highly complex method such as purity, assay, and impurity profiling for therapeutic oligos such as anti-sense oligonucleotides.In this presentation, we present a paradigm shift in software with Oligo Analysis Accelerator for OpenLab CDS. This application streamlines the analytical workflow by using a fit-for-purpose user interface while leveraging existing data integrity and compliance architecture within OpenLab CDS.<h4>By attending this webinar, you will:</h4><ul><li>Understand the fundamentals of ion-pair reversed-phase LC/MS for oligonucleotides</li><li>Discover how a single quadrupole detector is used for impurity profiling of anti-sense oligonucleotides</li><li>Learn how fit-for-purpose software for LC/MS analysis in QC lot release can save time and enhance quality</li></ul><h5>Who should attend</h5><ul><li>Analytical chemists interested in cutting-edge software for large molecule late-stage development</li><li>Analytical research and development labs interested in developing robust methods for single-stranded oligo therapeutics</li><li>Process scientists who rely on in-process control purity results to optimize synthesis and purification for oligos</li><li>Quality assurance and quality control personnel supporting oligo testing labs</li></ul><blockquote>Presenter: Brian Rivera (Software Product Manager, Agilent Technologies)Brian Rivera is a software product manager at Agilent Technologies with 20 years of experience in HPLC analysis of large molecules.</blockquote></div></div>

Separation Sciences: Boost Efficiency in Oligonucleotide QC with Software Designed for the Job

Boost Efficiency in Oligonucleotide QC with Software Designed for the Job

In this presentation, we present a paradigm shift in software with Oligo Analysis Accelerator for OpenLab CDS.

Oligonucleotide QC with Software Designed for the Job

Join us as we dive into a common challenge faced by research facilities: managing the ever-growing mountain of measurement data. As a facility manager, you’re all too familiar with the constant juggling act of handling sample submissions, data collection, and distribution—all while trying to keep your workflows efficient and your focus on the science.In today’s data-driven landscape, adhering to the FAIR principles—making your data Findable, Accessible, Interoperable, and Reusable—is not just a best practice but a growing requirement for securing public funding. Funding bodies are increasingly looking for research facilities to demonstrate that their data management processes align with these principles.That’s why we’re excited to introduce you to LOGS for Facilities, our comprehensive software solution tailored specifically for research facilities like yours. Supported by the powerful automation engine Mnova Gears, LOGS streamlines your entire workflow—from sample submission and automatic data processing to data distribution—into a single, seamless platform that adheres to the FAIR principles.In today’s session, we’ll explore how LOGS can simplify your data management processes, helping you save time, reduce errors, and get back to what matters most: advancing your research by making your data findable - wherever you are.<blockquote>Presenter: Peter Bellstedt, PhD (Director of Business Development and Product Manager)Peter studied Biochemistry at the University of Jena before completing a PhD on the structural characterization of a DNA repair protein using solution and solid-state NMR. He was then appointed as the head of the NMR Spectroscopy Platform in Jena, Germany, where he pioneered the implementation of modern, highly efficient data-driven workflows. After five years, he moved to the University of Zurich, Switzerland, as a principal investigator, where his lab focused on structure-based drug discovery. This year, he brought his wealth of experience to the LOGS by SciY team, focusing on efficient workflow solutions for research labs and facilities on a global scale.</blockquote>

Mestrelab Research: Integrating Measurement Requests, Data Collection, and Data Distribution in One Platform

Integrating Measurement Requests, Data Collection, and Data Distribution in One Platform

In today’s session, we’ll explore how LOGS can simplify your data management processes, helping you save time

LCMS webinars focusing on Software - page 20