<p><strong>Tasty beer starts with knowing what’s in your hops. Explore how online SPE makes bitter acid analysis in hops faster and more reliable.</strong></p><h4><strong>How can you detect bitter acid profiles quickly, reliably, and more sustainably?</strong></h4><p>For craft brewers and beer lovers alike, a refreshing experience is all about flavor.</p><p>So how can you achieve the desired bitterness in your beer? The answer is not just about the recipe. Finding the right flavor profile starts with the bitter acids present in the starting hops material.</p><div><div><p>Hops (the heart of your favorite beer) are the source of the brew’s distinctive flavor tied to bitterness and aroma.</p><p>At the molecular level, bitterness is driven by <a target="_blank" rel="noopener noreferrer" href="https://www.sciencedirect.com/science/article/pii/S0308814622016338">α- and β-bitter acids</a>, like humulones and lupulones, whose concentrations determine the final sensory profile of the beer (<strong>Figure 1</strong>).</p><p>Accurately characterizing these compounds in each batch allows brewers to unlock the full potential of every variety of hops.</p><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Thermo_Fisher_Scientific_How_Bitter_Acid_Analysis_Helps_Brewers_Better_Control_Beer_Flavors_2_6435757c03.jpeg" alt="Thermo Fisher Scientific: How Bitter Acid Analysis Helps Brewers Better Control Beer Flavors: Figure 1. General chemical structure of hop bitter acids." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Thermo_Fisher_Scientific_How_Bitter_Acid_Analysis_Helps_Brewers_Better_Control_Beer_Flavors_2_6435757c03.jpeg 222w," sizes="100vw" width="700" height="493"></p><p>While conventional analytical methods rely on long, manual sample preparation steps and hazardous solvents, laboratories now strive for greener and more efficient workflows.</p><p>And moving from manual to online sample preparation is one of the easiest ways your lab can reduce waste and — most importantly — improve reliability with automation.</p><h4><strong>Key takeaways</strong></h4><ul><li>Online SPE reduces hands-on work, shortens turnaround time, and supports more sustainable methods by cutting solvent use and eliminating single-use cartridges.</li><li>Automated UHPLC workflows are easier for new users to execute, offering more reproducible quantifications of α- and β-bitter acids in hops.</li><li>Accurate bitter acid profiles help brewers distinguish hop varieties and control flavor with confidence.</li></ul><h4><strong>Comparing offline vs. online solid phase extraction for bitter acid HPLC analysis</strong></h4><p>If you’ve spent long hours in the lab preparing samples for hops analysis, you’re not alone.</p><p>Manual sample preparation often takes the most time in hops analysis, an area where online <a target="_blank" rel="noopener noreferrer" href="https://www.thermofisher.com/content/lifetech/global/en/home/industrial/chromatography/chromatography-learning-center/chromatography-consumables/sample-prep.html">solid phase extraction</a> (SPE) can make a real difference. Here’s why.</p><p>Manual SPE has a lot of manual steps. Extraction. Cartridge loading. Washing. Elution. And sometimes solvent exchange or reduction. All before your actual analysis begins. This labor-intensive workflow can take up to an hour and depends strongly on operator skillsets, making reproducibility a challenge for new hires.</p><h4><strong>Online SPE analysis provides key benefits like:</strong></h4><ul><li>Hands-off workflow free of cartridges, manifolds, or vacuum steps.</li><li>Column washing and re-equilibration occur in parallel with separation.</li><li>SPE step is complete in just two minutes.</li><li>Minimizes operator-dependent variability.</li><li>Uses less than 8 mL of solvent.</li></ul><p>Let’s explore what this technique looks like in practice.</p><h4><strong>Online SPE for analyzing α- and β-bitter acids in hops standard solutions.</strong></h4><p>Changing your sample preparation from manual to automated isn’t complicated. With the right tools you can fully integrate online SPE with your UHPLC system (<strong>Figure 2</strong>).</p><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Thermo_Fisher_Scientific_How_Bitter_Acid_Analysis_Helps_Brewers_Better_Control_Beer_Flavors_3_4fb14243b4.jpeg" alt="Thermo Fisher Scientific: How Bitter Acid Analysis Helps Brewers Better Control Beer Flavors: Figure 2. Schematic workflow for the determination of bitter acids in hops. The process involves clean up and instrumental analysis using the Thermo Scientific Vanquish Online SPE UHPLC System." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Thermo_Fisher_Scientific_How_Bitter_Acid_Analysis_Helps_Brewers_Better_Control_Beer_Flavors_3_4fb14243b4.jpeg 245w," sizes="100vw" width="700" height="323"></p><h4><strong>The process for analyzing a hops standard solution is straightforward:</strong></h4><ol><li>First, extract hop samples with ethanol (a greener alternative to toluene or diethyl ether in conventional methods).</li><li>Inject the extract directly into your instrument for analysis. Bitter acids retain<a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/products/1970"><strong> on the SPE column</strong></a> while matrix interferences that can comprise quantitation are removed.</li><li>Next, backflush the SPE column to transfer the analytes to the<a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/products/423"><strong> analytical column </strong></a>for separation. While this separation is taking place, the SPE column is washed and re-equilibrated in parallel, keeping the total run time to 15 minutes, with the separation of all six major bitter acids in a single analysis (<strong>Figure 3</strong>).</li></ol><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Thermo_Fisher_Scientific_How_Bitter_Acid_Analysis_Helps_Brewers_Better_Control_Beer_Flavors_4_1e47405e3c.jpeg" alt="Thermo Fisher Scientific: How Bitter Acid Analysis Helps Brewers Better Control Beer Flavors: Figure 3. UV chromatogram of hop bitter acids in standard solution (1-cohumulone, 2-humulone, 3-adhumulone, 4-colupulone, 5-lupulone, 6-adlupulone)." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Thermo_Fisher_Scientific_How_Bitter_Acid_Analysis_Helps_Brewers_Better_Control_Beer_Flavors_4_1e47405e3c.jpeg 245w," sizes="100vw" width="700" height="347"></p><h4><strong>Online SPE for analyzing α- and β-bitter acids in real hops samples</strong></h4><p>Analytical labs need methods that are accurate and reliable for brewers to develop distinct and reproducible flavor profiles across batches.</p><p>To assess the applicability of online SPE UHPLC methods for hops analysis, we analyzed bitter acid profiles from four species — Sultana, Barbe Rouge, Azacca, and Sabro.</p><p>The concentration of α-acids dominated all samples, while each hops species exhibited distinct profiles in terms of α-acid and β-acid content, making them suitable for different brewing strategies (<strong>Figure 4</strong>).</p><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Thermo_Fisher_Scientific_How_Bitter_Acid_Analysis_Helps_Brewers_Better_Control_Beer_Flavors_5_3a179217db.jpeg" alt="Thermo Fisher Scientific: How Bitter Acid Analysis Helps Brewers Better Control Beer Flavors: Figure 4. Bitter acid profiles in four different hop species, error bars display SD (n=6). To check method consistency, we measured each hops extract six times. Variation between measurements was low, with relative standard deviation below 3.5 % across all hops species." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Thermo_Fisher_Scientific_How_Bitter_Acid_Analysis_Helps_Brewers_Better_Control_Beer_Flavors_5_3a179217db.jpeg 245w," sizes="100vw" width="700" height="410"></p><ul><li>For testing labs, this process translates into a workflow that is easy to run, fast, and delivers reliable results without added complexity.</li><li>For craft brewers, reliability means clear, trustworthy insights into hops bitter acid profiles, for better hop selection and more precise control of bitterness and flavor in your beer.</li></ul><h4><strong>From carefully procured samples to tasty sips</strong></h4><p>Efficient UHPLC workflows support faster results, less solvent use, and reliable bitterness data for brewers. And online SPE turns those advantages into practical, day-to-day benefits for brewers and labs alike.</p><ul><li>Less hands-on skills required for lab analysts</li><li>Faster results from labs to brewers</li><li>A more sustainable way to work</li><li>Clear insights into hops flavor</li></ul><p>To learn more about the method and see the full application data, <a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/labrulez-bucket-strapi-h3hsga3/cn_004238_hplc_vanquish_online_spe_bitter_acids_hops_cn004238_na_en_587a5d300b.pdf"><strong>check out our application note today</strong></a>.</p><h4><strong>FAQs</strong></h4><div><div><h5><strong>What are the key compounds in hops analyzed by HPLC?</strong></h5><p>Bitter acids, namely α-acids and β-acids, especially the six major bitter acids (humulones and lupulones) that define hop bitterness and are required for quality control and brewing decisions.</p></div><div><h5><strong>What column types are recommended for hop acid separations?</strong></h5><p>Classical C18 Silica columns are ideal for bitter acids separation. UHPLC compatible columns with particle size ≤ 2 µm boost analysis with higher flow rate, thus shorter analysis time and solvent consumption.</p></div><div><h5><strong>What’s the best workflow for hops analysis?</strong></h5><p>A typical workflow for hop analysis consists of three main steps: extraction of the target compounds, clean-up of co-extracted matrix components, and instrumental analysis by HPLC or UHPLC. To keep this workflow efficient and reproducible, you should minimize manual handling and add in automation when possible. Integrating online SPE into your LC instrument allows sample clean-up to run automatically, reducing hands-on time, solvent use, and variability while delivering faster, more reliable results.</p></div></div><blockquote><p><strong>Visit us on&nbsp;</strong><a target="_blank" rel="noopener noreferrer" href="https://www.linkedin.com/showcase/72003132/admin/dashboard/"><strong><u>LinkedIn:</u></strong></a>&nbsp;#UHPLC #Automation</p></blockquote></div></div>

How Bitter Acid Analysis Helps Brewers Better Control Beer Flavors

<p><strong>In the week of June 29, the following webinars await you in the field of liquid phase and especially HPLC and LC/MS.</strong></p><blockquote><h4><a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/webinars"><strong>👉 DETAILS AND REGISTRATION IN THE WEBINARS SECTION</strong></a></h4><p><strong>👉 Search and filter in the largest global database</strong> with over <strong>5 500 GC, LC, MS and spectroscopy webinars</strong>.</p></blockquote><h5><strong>1. Thermo Fisher Scientific: Insights from an IC specialist: To be suppressed or not suppressed?</strong></h5><ul><li>Tu, 30.6.2026 09:00 CEST</li><li><a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/webinar/6310">Registration</a></li></ul><p>Join Thermo Fisher’s Ask the Experts session to learn how suppressor technology impacts ion chromatography performance, sensitivity, reliability, maintenance, and troubleshooting.</p><h5><strong>2. SCIEX: Advancing Proteomics with ZenoTOF: From Single-Cell Analysis to Plasma Biomarker Discovery</strong></h5><ul><li>Tu, 30.6.2026 11:30 CEST</li><li><a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/webinar/6311">Registration</a></li></ul><p>Discover how ZenoTOF high-resolution mass spectrometry is accelerating single-cell proteomics and plasma biomarker discovery through enhanced sensitivity, throughput, and automation.</p><h5><strong>3. Agilent Technologies: Measuring Diverse Samples with UV/VIS Spectrometers and Integrating Spheres</strong></h5><ul><li>Tu, 30.6.2026 20:30 CEST</li><li><a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/webinar/6052">Registration</a></li></ul><p>This webinar will discuss diffuse transmission and reflection UV/VIS measurements, including how to perform measurements with Agilent’s Cary 60 UV/VIS Spectrometer.</p><h5><strong>4. Waters Corporation: Informatics series #33 Tips, Tricks and Best Practices</strong></h5><ul><li>Th, 2.7.2026 11:00 CEST</li><li><a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/webinar/6315">Registration</a></li></ul><p>This interactive session revisits essential features, shortcuts, and practical techniques in Empower software that can streamline everyday laboratory work and reduce unnecessary effort.</p><h5><strong>5. Waters Corporation: Unlocking Therapeutic Peptide Characterisation (GLP-1RAs) with Cyclic IMS</strong></h5><ul><li>Th, 2.7.2026 15:00 CEST</li><li><a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/webinar/6313">Registration</a></li></ul><p>Discover how cyclic ion mobility spectrometry (cyclic IMS) improves characterization of GLP-1 analogues and therapeutic peptides, supporting development, quality control, and regulatory submissions.</p><h5><strong>6. Agilent Technologies: Spectral Flow Cytometry Masterclass - Session 1</strong></h5><ul><li>Fr, 3.7.2026 07:00 CEST</li><li><a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/webinar/6316">Registration</a></li></ul><p>Learn how full-spectrum flow cytometry enables high-dimensional cell analysis. Explore spectral flow technology, experimental design, and applications in immunology, oncology, and vaccine research.</p>

Webinars LabRulezLCMS Week 27/2026

<p><strong>The international standard ISO/IEC 17025 [1] clause 7.7.2 with regards to ensuring the validity of results, requires a laboratory to participate in proficiency testing (PT) and/or to participate in interlaboratory comparisons (ILCs) other than PT. However, no guidance as to what these other ILCs might be, or indeed how they might compare in relation to PT, is provided. This leaflet aims to outline other ILCs available, pointing out some of the limitations as compared to PT.&nbsp;</strong></p><p>There are three major types of ILCs in which a group of laboratories analyses identical portions from a homogeneous, stable test sample, with each type being characterized by its intended purpose [2,3]:</p><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Eurachem_Interlaboratory_comparisons_other_than_proficiency_testing_2_d9263807fb.jpg" alt="Eurachem: Interlaboratory comparisons other than proficiency testing" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Eurachem_Interlaboratory_comparisons_other_than_proficiency_testing_2_d9263807fb.jpg 245w," sizes="100vw" width="1507" height="607"></p><p>A key point is that PT is an ILC that is designed to specifically evaluate the performance of participants, providing them with an infrastructure to monitor and assess the validity of their results. This is not the key purpose of the two other ILCs, and thus depending on their intended purpose they have a number of limitations that laboratories need to be aware of when using them rather than PT. Only proficiency testing should have been conducted in full compliance to the competency standard ISO/IEC 17043 [4].&nbsp;</p><h4><strong>Evaluation of measurement procedure performance&nbsp;</strong></h4><p>These ILCs, are designed to assess how a particular measurement procedure performs and whether it is fit for its intended purpose. These ILCs are often referred to as a ‘method performance study’ or a ‘collaborative study’ [2]. Some limitations of using such ILCs for monitoring laboratory performance as an alternative to PT are:&nbsp;</p><ul><li>All participants use the same measurement procedure i.e. the measurement procedure that is being validated. Unless the measurement procedure is being routinely used by the participating laboratory such an ILC will not assess the routine performance of the laboratory using their own routine measurement procedure. Nor does it enable them to compare their performance with other laboratories using alternative measurement procedures.&nbsp;</li><li>The measurement procedure is likely to be new, so participants may not have established stable procedures and quality control, so performance may not reflect routine use.&nbsp;</li><li>The report from such an ILC would not provide information on the performance of the participant, if the statistical model used makes the assumption that all laboratories perform with equal variability.</li><li>The design of the study may require the participants to assess multiple samples and/or to undertake multiple replicate analysis on the test materials. This may not be representative of the routine measurements or tests undertaken by the laboratory.&nbsp;</li><li>The conclusion of the ILC might be that the measurement procedure being assessed is not valid.&nbsp;</li></ul><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Eurachem_Interlaboratory_comparisons_other_than_proficiency_testing_3_a53ecd2f5d.jpg" alt="Eurachem: Interlaboratory comparisons other than proficiency testing" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Eurachem_Interlaboratory_comparisons_other_than_proficiency_testing_3_a53ecd2f5d.jpg 157w," sizes="100vw" width="570" height="566"></p><h4><strong>Characterisation of candidate reference materials&nbsp;</strong></h4><p>These are ILCs that assign a property value to a candidate reference material, usually with a stated measurement uncertainty. These are often referred to as a ‘material certification study’ [2]. Limitations of using such ILCs as an alternative to PT are:&nbsp;</p><ul><li>The analysis of the candidate reference material is likely to require additional care in exercising the routine measurement procedure, such as substantially more replication, and/or may require quite different reporting formats (e.g. all valid replicates instead of a mean result). Thus, such an ILC will not necessarily provide an evaluation of the performance of the laboratory based on the routine use of their measurement procedures.&nbsp;</li><li>The report from such an ILC may include the results from the participating laboratories but would not provide information on the performance of each laboratory.&nbsp;</li><li>Participating laboratories may be required to assess multiple samples and/or to undertake multiple replicate analysis on the candidate reference materials. Thus this may not be representative of the routine measurements or tests undertaken by the laboratory.&nbsp;</li></ul><h4><strong>Other ILCs&nbsp;</strong></h4><p>Some other ILCs, whilst not being conducted in full compliance of the requirements of ISO/IEC 17043, may be designed to evaluate participant performance. These would include small ILCs as defined by EA‑2/21 [5] or split sample ILCs i.e. where a portion of a single sample is sent to two or three laboratories. Whilst these ILCs are specifically designed to evaluate participant performance, they may not provide a comprehensive comparison that a PT might provide. In such cases the laboratory needs to evaluate carefully the competence of the organization managing the ILC and the suitability of the service externally provided (ISO/IEC 17025 clause 6.6).</p>

Interlaboratory comparisons other than proficiency testing

Article

Webinars

Academy

A unique company dedicated to serving science helping our customers drive productivity and accelerate results. Through our premier brands – Thermo Scientific, Applied Biosystems, Invitrogen, Fisher Scientific and Unity Lab Services.

Thermo Fisher Scientific

Eurachem is a European network promoting international traceability of chemical measurements and good analytical practice. It provides expert guidance, supports method validation, uncertainty evaluation, proficiency testing, and facilitates discussion on technical and policy issues in analytical quality.

Eurachem

Everything from the world of analytical chemistry in one place. We connect people in solving their problems. At Labrulez you will find all the necessary information easily, quickly and clearly. Stop searching and start finding.

LabRulez

New Food magazine

Technology Networks

Bruker

Wiley

SCIEX

Evosep Biosystems

<p><strong>This online event has been designed for our customers and brings together opinion leaders, top scientists and industry innovators to share their research in the biopharma field.</strong></p>
<p>The technologies presented will be CE and LC-MS.</p>
<p>Agenda:</p>
<h5><strong>10:00–10:10 Welcome and introduction</strong></h5>
<ul>
<li>Steve Lock, SCIEX</li>
</ul>
<h5><strong>10:10–10:35 Use of LC-MS in process analytical testing as part of development of a continuous manufacturing workflow</strong></h5>
<ul>
<li>Daniel Myatt / Ronan Gough, CPI</li>
</ul>
<h5><strong>10:35–11:00 Tips and tricks for biotherapeutics characterization by peptide mapping</strong></h5>
<ul>
<li>Giulia Calloni, SCIEX</li>
</ul>
<h5><strong>11:00–11:25 Developing methods for SEC-MS and IEX-MS for the analysis of biotherapeutics</strong></h5>
<ul>
<li>Sarah Nguyen, Strasbourg University</li>
</ul>
<h5><strong>11:25–11:35</strong> Break</h5>
<h5><strong>11:35–12:00 The power of CZE analysis for characterization of NANOBODY® molecules</strong></h5>
<ul>
<li>Dries Dejaegere, Sanofi</li>
</ul>
<h5><strong>12:00–12:25 Multi-capillary gel electrophoresis to increase throughput for the purity analysis of biotherapeutic proteins</strong></h5>
<ul>
<li>Theresa Merlino, The Janssen Pharmaceutical Companies of Johnson &amp; Johnson</li>
</ul>
<h5><strong>12:25–12:50 A disruptive approach to the characterization of mAb charge variants during biopharmaceutical process development</strong></h5>
<ul>
<li>Greg Adams, FUJIFILM</li>
</ul>
<h5><strong>12:50–13:00 Wrap-up and Q&amp;A</strong></h5>
<blockquote>
<p><strong>Presenter:</strong> Steve Lock, PhD (Snr. Marketing and Market Development Manager, Biopharma, EMEAI, SCIEX)</p>
</blockquote>
<blockquote>
<p>Steve obtained his PhD in Physical Organic Chemistry from the University College of Swansea in 1993 and is a chartered chemist and a member of the Royal Society of Chemistry. He has worked for SCIEX in various technical roles for over 20 years and has presented at over 80 international meetings around the world. He currently works closely with customers in the biopharmaceutical industry. Steve is also currently an Adjunct Professor at the University of Pannonia.</p>
</blockquote>
<p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/SCIEX_agenda_day_1_6818ee0331/SCIEX-agenda-day-1.jpg" alt="SCIEX - agenda day 1.jpg"/></p>


SCIEX: SCIEX Biopharma meeting - 3rd edition! Day 1 - Gene therapy, vaccines and oligonucleotides

SCIEX Biopharma meeting (Day 1) - Gene therapy, vaccines and oligonucleotides

Gene therapy, vaccines and oligonucleotides. The technologies presented will be CE and LC-MS.

Day 1 - Gene therapy, vaccines and oligonucleotides

<p><strong>This online event has been designed for our customers and brings  together opinion leaders, top scientists and industry innovators to share their research in the biopharma field.</strong></p>
<p>The technologies presented will be CE and LC-MS.</p>
<p>Agenda:</p>
<h5><strong>10:00–10:10 Welcome and introduction</strong></h5>
<ul>
<li>Steve Lock, SCIEX</li>
</ul>
<h5><strong>10:10–10:35 Particle seizing by CE</strong></h5>
<ul>
<li>Hervé Cottet, Montpellier University</li>
</ul>
<h5><strong>10:35–11:00 Application of CE for purity analysis in cell and gene therapy</strong></h5>
<ul>
<li>Ryan Hylands / Luiza Chrojan, Pharmaron</li>
</ul>
<h5><strong>11:00–11:25 Tips and tricks for success in CE analysis</strong></h5>
<ul>
<li>Whitney Smith, SCIEX</li>
</ul>
<h5><strong>11:25–11:35</strong> Break</h5>
<h5><strong>11:35–12:00 RNA payload analysis: development and analytical challenges</strong></h5>
<ul>
<li>Jérémie Parot, SINTEF Industry</li>
</ul>
<h5><strong>12:00–12:25 The use of CE for AAV analytics</strong></h5>
<ul>
<li>Padraig Flaherty, MeiraGTx</li>
</ul>
<h5><strong>12:25–12:50 Oligo analysis by LC-MS</strong></h5>
<ul>
<li>Alexandra Bushby, Labcorp</li>
</ul>
<h5><strong>12:50–13:00 Wrap-up and Q&amp;A</strong></h5>
<blockquote>
<p><strong>Presenter:</strong> Steve Lock, PhD (Snr. Marketing and Market Development Manager, Biopharma, EMEAI, SCIEX)</p>
</blockquote>
<blockquote>
<p>Steve obtained his PhD in Physical Organic Chemistry from the University College of Swansea in 1993 and is a chartered chemist and a member of the Royal Society of Chemistry. He has worked for SCIEX in various technical roles for over 20 years and has presented at over 80 international meetings around the world. He currently works closely with customers in the biopharmaceutical industry. Steve is also currently an Adjunct Professor at the University of Pannonia.</p>
</blockquote>
<p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/SCIEX_agenda_day_2_b442145213/SCIEX-agenda-day-2.jpg" alt="SCIEX - agenda day 2.jpg"/></p>


SCIEX: SCIEX Biopharma meeting - 3rd edition! Day 2 - Therapeutic proteins and peptides

SCIEX Biopharma meeting (Day 2) - Therapeutic proteins and peptides

Therapeutic proteins and peptides. The technologies presented will be CE and LC-MS.

Day 2 - Therapeutic proteins and peptides

<p><strong>During drug metabolite identification (Met ID) studies collision-induced dissociation (CID) is the most commonly used fragmentation technique for LC-MS experiments.</strong></p>
<p>Many conjugation metabolites are found to have multiple bonding potentials to parent drugs. It is a great challenge to locate the bonding sites by CID due to the information lost by highly selective cleavage on these bonds. </p>
<p>The novel electron-activated dissociation (EAD) is a recently developed technology on the SCIEX ZenoTOF 7600 system. From a single LC-MS/MS experiment CID and EAD data can be collected, providing information rich fragmentation spectra. The additional information can help in confident localization and identification of metabolites compared to CID alone. These fragments can be crucial to locate the metabolic modification sites, such as conjugations. </p>
<p>In this study, the application of EAD in drug metabolite profiling is explored. The efficiency in the structural elucidation of conjugation metabolites is compared with that of CID.</p>
<p>Webinar Learning Objectives:</p>
<ul>
<li><p><strong>Learn how EAD can be advantageous in confident identification and localization of drug metabolites</strong></p>
</li>
<li><p><strong>Discover the sensitivity of the Zeno trap technology in the identification of the low abundant metabolites</strong></p>
</li>
<li><p><strong>Explore the capabilities of the Molecule Profiler software in processing EAD and CID data for drug metabolite identification</strong></p>
</li>
</ul>
<blockquote>
<p><strong>Presenter:</strong> Ming Yao (Principal Scientist, CPPDB, Bristol-Myers Squibb)</p>
</blockquote>
<blockquote>
<p>Ming Yao currently works at Bristol-Myers Squibb (BMS). He received his B.Sc. from Henan University, and his M.Sc. from the Chinese Academy of Preventive Medicine China. After graduation, he worked at the Institute of Occupational Medicine as an associate professor.</p>
</blockquote>
<blockquote>
<p>As a visiting Associate Professor, he conducted research for bioactivation of chemicals at Merck as well as the University of Western Ontario. After then, he joined BMS and he was responsible for designing and conducting in vitro and in vivo biotransformation studies for multiple programs to support both development and discovery projects. Most recently, he has also been involved in the development of protein drugs, such as the antibody drug conjugates (ADCs) and biotransformation of biologics.</p>
</blockquote>
<blockquote>
<p>Ming has over 28 years of experiences in the pharmaceutical industry and has over 50 publications and 5 book chapters covering new technologies of mass spectrometry and its applications in drug metabolism.</p>
</blockquote>
<blockquote>
<p><strong>Presenter:</strong> Rahul Baghla (Manager Pharma Applications, SCIEX)</p>
</blockquote>
<blockquote>
<p>Rahul Baghla, Manager Pharma Applications is a research scientist with 12+ years of experience in various applications of mass spectrometry delivering in Pharma/Biopharma/CRO industries. He is specialised in implementing new mass spectrometry technologies in bioanalytical/analytical method development/validation, drug discovery, ADME assays and clinical studies.</p>
</blockquote>
<blockquote>
<p>He has supported various functions within pharma industry including nutrition, wellness, and oral care based clinical trial studies over the years. Rahul has master’s degree in pharmaceutical analysis, master’s in business administration and a professional diploma in clinical research.</p>
</blockquote>
<blockquote>
<p>He is responsible for continuous engagement with pharmaceutical community to identify major analytical challenges, provide solutions and to find significant analytical opportunities for SCIEX.</p>
</blockquote>


SCIEX: Discover how Bristol-Myers Squibb uses electron-activated dissociation (EAD) to confidently identify drug metabolites and localize site of metabolism.

Discover how Bristol-Myers Squibb uses electron-activated dissociation (EAD) to confidently identify drug metabolites and localize site of metabolism.

Learn how EAD can be advantageous in confident identification and localization of drug metabolites.

LC/MS EAD & drug metabolites & localization of metabolism

<p><strong>Over the last few years, spatial biology has refined the understanding of coordinated biological processes that govern how systems both thrive and become vulnerable to disease.</strong></p>
<p>The latest tools offer advanced images that track the location and types of proteins and RNA across tissue microdomains and cellular phenotypes using a variety of detection techniques but lack the ability to see other post-transcriptional processes defined by metabolites and glycans. MALDI Imaging is a true multiomics technique that allows for spatial determination of metabolites and lipids in tissue, as well as expanding targeted analysis to small molecules, glycans, and proteins. The addition of MALDI-2 and microGRID technology enables imaging for maximum biological information at previously unreachable sensitivity and robust (sub)cellular resolution.</p>
<p>This webinar will introduce how Bruker’s new MALDI HiPLEX-IHC Imaging workflow delivers greater speed and field-of-view for multiplexed protein imaging in tissue, while providing thousands of untargeted molecular images that enable deeper tissue subtyping. The combination of protein spatial localization combined with metabolites and glycans offers a new pathway for coordinated biomarker discovery to complement existing spatial techniques.</p>
<p>What to expect?</p>
<ul>
<li><strong>Live presentation from Bruker’s experts to see ease of use of the timsTOF fleX platform, new MALDI HiPLEX-IHC workflows and highlighting user stories, including focus on improved spatial resolution with microGRID, improved sensitivity with MALDI-2, improved separation selectivity with trapped ion mobility and comprehensive software solutions</strong></li>
</ul>
<p>Who should attend?</p>
<ul>
<li><p>Anyone using or interested in using tools for spatial biology discovery or translation</p>
</li>
<li><p>Scientists &amp; researchers interested in expanding their workflows to the spatial dimension, scientists working with spatial distribution of small molecule biologics</p>
</li>
<li><p>MALDI Imaging users wanting to expand their workflows</p>
</li>
<li><p>Anyone interested in learning more about MALDI Imaging capabilities</p>
</li>
</ul>
<blockquote>
<p><strong>Presenter:</strong> Kate Stumpo, Ph.D. (Senior Market Manager Imaging, Bruker, Billerica, MA, USA)</p>
<ul>
<li>Introduction and welcome to MALDI Imaging </li>
<li>Spatial biology meets Mass Spectrometry in cancer research </li>
<li>MALDI Imaging utility in neurological research</li>
</ul>
</blockquote>
<blockquote>
<p><strong>Presenter:</strong> Corinna Henkel, Ph.D. (Application Scientist Imaging, Bruker, Bremen, Germany)</p>
<ul>
<li>MALDI HiPLEX-IHC workflow technical introduction</li>
<li>Software solutions for multiomics integration</li>
</ul>
</blockquote>
<blockquote>
<p><strong>Presenter:</strong> Bjoern Wendik, Ph.D. (Business Development Manager, Bruker, Bremen, Germany)</p>
<ul>
<li>Bridging non-MS imaging to the MALDI world</li>
<li>Automation, workflow and relevance for future clinical applications</li>
</ul>
</blockquote>


Bruker: Redefining Hiplexed Spatial Multiomics

Redefining Hiplexed Spatial Multiomics

How Bruker’s new MALDI HiPLEX-IHC Imaging workflow delivers greater speed and field-of-view for multiplexed protein imaging in tissue.

<p><strong>Join this webinar to hear from Dr. Karl Oetjen and Dr. Craig Butt, who will share their recent research on nontargeted analysis of food products.</strong></p>
<p>Discover how advanced analytical methods can detect unlisted, unexpected, and unwanted ingredients, leading to increased confidence throughout the supply chain, ultimately benefiting consumers.</p>
<p>This webinar looks at several sample types in the application of diagnostic ion screening to prioritise nontarget features for food safety analysis. Data from this study includes the analysis of supplements products from North American Gas Stations to determine what are the actual ingredients that “boost metabolism”, “elevates mood” and supply “good luck energy”. This study also looks at saffron and its origins along with an update on nontargeted screening of pesticides in food samples.</p>
<p>Discover the ingredients behind marketing slogans.</p>
<ul>
<li><p><strong>Understand why ZenoTOF technology is at the forefront of detecting the unknown, unexpected and unwanted at trace levels.</strong></p>
</li>
<li><p><strong>Discover how mass spectrometry can be used to determine authenticity of expensive ingredients.</strong></p>
</li>
<li><p><strong>Learn how nontargeted workflows can test the regulated and the non-regulated in pesticide assays.</strong></p>
</li>
<li><p><strong>A chance to ask our expert speakers questions and these exciting workflows.</strong></p>
</li>
</ul>
<blockquote>
<p><strong>Presenter:</strong> Karl Oetjen (Senior Scientist, Technical Marketing, SCIEX)</p>
</blockquote>
<blockquote>
<p>Karl Oetjen is a Senior Scientist at SCIEX in the technical marketing group and has over 10 years of mass spectrometry experience. Before joining SCIEX, he completed his PhD at Colorado School of Mines (Golden, Colorado) in Hydrologic Science and Engineering under Dr. Chris Higgins. Karl’s dissertation research focused on nontarget characterisation of complex surfactant mixtures, including aqueous film forming foams. This work led to the discovery several novel per- and polyfluorinated alkyl substances (PFAS) that since have been found in variety of environmental samples and industrial chemicals. Since joining SCIEX, Karl has worked with numerous labs creating and implementing both regulated and unregulated quantitative and qualitative screening methods.  </p>
</blockquote>
<blockquote>
<p><strong>Presenter:</strong> Craig Butt (Manager, Applied Markets, SCIEX)</p>
</blockquote>
<blockquote>
<p>Craig M. Butt is Manager of the Applied Markets group in the Global Technical Marketing division of SCIEX with over 20 years of mass spectrometry experience.  Previously at SCIEX he was an applications scientist where he developed methods for targeted and non-targeted methods in the cannabis, environmental, food and beverage group.  He obtained his Ph.D. in environmental chemistry at the University of Toronto, his thesis research investigated the fate of poly- and perfluorinated organic compounds (PFAS) in biological systems.  He was a NSERC post-doctoral research fellow in the Nicholas School of the Environment at Duke University, later becoming a research scientist in the department.</p>
</blockquote>


New Food:Enhancing food safety analysis with diagnostic Ion screening

Enhancing food safety analysis with diagnostic Ion screening

Several sample types in the application of diagnostic ion screening to prioritise nontarget features for food safety analysis.

<p><strong>Exposomics research studies the impact of environmental factors, including exposure to contaminants, on health.</strong></p>
<p>Untargeted workflows for metabolomics and exposomics typically report hundreds of features belonging to diverse compound classes, identified with varying levels of confidence.</p>
<p>In this webinar, our expert speaker will discuss how to optimize your untargeted approaches on Zeno SWATH DIA using a modular workflow. With this approach, each subset of the full mass range can be individually adjusted to meet project-specific and user-defined goals, enhancing the performance and adaptability of untargeted MS tools for exposomics research.</p>
<p>Attend this webinar to: </p>
<ul>
<li><p><strong>Discover exposomics, the insights it provides into human health and the role of mass spectrometry in this field</strong></p>
</li>
<li><p><strong>Learn a rational approach to optimize chromatography and mass spectrometry parameters</strong></p>
</li>
<li><p><strong>Make your method development efforts modular so they can be reused to meet different project-specific needs</strong></p>
</li>
<li><p><strong>Find out how using Zeno SWATH DIA can benefit your metabolomics or exposomics research.</strong></p>
</li>
</ul>
<blockquote>
<p><strong>Presenter:</strong> Dr. Cristina Balcells Nadal (Research Associate, Imperial College London)</p>
</blockquote>


Technology Networks: Optimizing SWATH-DIA for Exposomics and Metabolomics

Optimizing SWATH-DIA for Exposomics and Metabolomics

How to optimize your untargeted approaches on Zeno SWATH DIA using a modular workflow.

<p><strong>The 1st key-note presentation will explore the pursuit of enhanced chromatographic performance in low-flow applications.</strong></p>
<p>Microfluidic pillar array columns offer a compelling alternative to traditional packed beds and monolithic columns, resulting in microfluidic separation beds with nanometer-scale control over geometry, enhancing quantitation accuracy and precision. The presentation focuses on a 5.5 cm separation channel housing radially elongated rectangular pillar shapes, ideal for high-throughput chromatographic separations. Integrated with advanced LC-MS instruments, the Vanquish Neo UHPLC system, and the latest Orbitrap mass spectrometers, this configuration achieves unparalleled quantitation accuracy and precision, handling up to 300 samples daily. </p>
<p>The second part will take us to detection technologies. HPLC has seen the advent and often also decline of various techniques which were claimed to enable universal detection. The emphasis will be on the use of an instrumental technique that was published specifically for charged aerosol detection (CAD) and is referred to as inverse gradient with the goal to widely eliminate the influence of the mobile phase composition on the analyte response. Practical examples will address extractables &amp; leachables, but also very common excipients in biopharmaceutical formulations. The method uses multiple orthogonal detectors (UV, CAD, MS) combined with the inverse gradient technique to facilitate analysis of a chemically diverse range of analytes. </p>
<p>Learning points:</p>
<ul>
<li><p><strong>How to achieve unparalleled quantitation accuracy and precision in high-throughput chromatographic separations</strong> </p>
</li>
<li><p><strong>Detection technologies</strong></p>
</li>
</ul>
<p>Who should attend:</p>
<ul>
<li><p>Lab managers and directors </p>
</li>
<li><p>Principal scientists </p>
</li>
<li><p>Laboratory analysts</p>
</li>
</ul>


Thermo Scientific: Leveraging innovative stationary phase architectures and multi-detection strategies in biopharma and proteomics research to enhance precision, efficiency, and results

Leveraging innovative stationary phase architectures and multi-detection strategies in biopharma and proteomics research to enhance precision, efficiency, and results

We will explore the pursuit of enhanced chromatographic performance in low-flow applications. The second part will take us to detection technologies.

Stationary phase & multi-detection in biopharma & proteomics

<p><strong>This webinar is your guide to get started with a fast and efficient, automated sample preparation protocol on the Opentrons OT-2.</strong></p>
<p>Utilize protein aggregation capture (PAC) on magnetic microparticles in an end-to-end  standardized and hands-off workflow, starting from protein lysate or plasma to peptide loaded on Evotips.</p>
<h4>Why choose an automated workflow?</h4>
<p>Automated sample preparation workflows have become a popular tool in proteomics laboratories for a number of reasons. With increased sample throughput on the Evosep One, you can prioritize numerous technical and biological replicates, adding robustness to downstream bioinformatic analyses.</p>
<p>By adopting full automation, you can gain precise control over protocols, ensuring reproducibility across samples and experiments. This minimizes batch effects, resulting in more reliable data for analysis and interpretation.</p>
<p>Furthermore, enhanced automation significantly reduces manual labor, enhancing reproducibility and providing researchers with more time for critical decision-making. This workflow also proves cost-efficient for large-scale applications, optimizing protein input for digestion.</p>
<h4>AUTOMATED WORKFLOW FOR INTEGRATED SAMPLE DIGESTION AND EVOTIP LOADING FOR HIGH-THROUGHPUT CLINICAL PROTEOMICS</h4>
<blockquote>
<p><strong>Presenter:</strong> Anders H.Kverneland (MD &amp; Postdoctoral researcher at Center for Cancer Immune Therapy, Herlev hospital)</p>
</blockquote>
<p>Protein identification and quantification is an important tool for biomarker discovery. With the increased sensitivity and shortened the analysis tim​e of modern mass spectrometers, sample-preparation remains a bottleneck and source of variability when studying large cohorts. To address this issue, we evaluated the use of a simple integrated workflow on the Opentrons OT-2 robot enabling both sample digestion and Evotip loading. Our results demonstrate high sensitivity combined with both reproducibility and stability even with very low sample inputs. The integration of the digestion and Evotip loading also strongly reduced the amount of reagents needed for the sample preparation. Using the integrated workflow, we evaluate the effects of cancer immune therapy on the plasma proteome in metastatic melanoma patients.</p>
<h4>STANDARDIZED AND FULLY AUTOMATED DEEP PROTEOME PROFILING OF PLASMA INTEGRATED WITH EVOSEP ONE ENABLES LARGE-SCALE CLINICAL COHORT ANALYSIS</h4>
<blockquote>
<p><strong>Presenter:</strong> Dorte Becker-Jensen (Application Manager at Evosep)</p>
</blockquote>
<p>This streamlined end-to-end workflow enables deep plasma profiling through the automated enrichment of membrane-bound vesicles and depletion of abundant proteins from a mere 4 μl of plasma. Integrated with the SCIEX ZenoTOF, it consistently identifies more than 3,500 protein groups, all with the ease of processing 30 samples per day at the push of a button.</p>


Evosep: Elevate efficiency: The power of automation

Elevate efficiency: The power of automation

Get started with a fast and efficient, automated sample preparation protocol on the Opentrons OT-2.

<p><strong>In this webinar, we will present end-to-end solutions for biopharma lab workflows with a focus on multiple mass spectrometry- based analysis techniques, ranging from SQ, TQ, TOF and Q-TOF.</strong></p>
<p>A broad portfolio in sample prep automation for protein therapeutics allows for highly reproducible results in the starting point of the analysis. A broad range of consumables and columns will be presented, leading into the subsequent separation via HPLC and detection by mass spectrometry.</p>
<p>The 6545XT AdvanceBio was particularly developed for intact protein analysis, and the ultra-high vacuum and large molecule autotune led to excellent sensitivity in denatured and native applications, allowing quantitation on the intact protein level as well as ADR calculations. Peptide mapping and glycan analysis results, along with the confirmation of oligonucleotide sequences using Q-TOF-based workflows, will be presented.</p>
<p>We will also highlight the ultrahigh performance of the novel 6495 triple quadrupole LC/MS system (G6495D) with MassHunter Workstation 12.1 for comprehensive multiplexed protein quantification. Artificial intelligence (AI)-based SWARM autotune and enhanced system features provide seamless protein quantification method optimization, ultimate analytical sensitivity, and high analytical precision as well as user-friendly instrument care and maintenance.</p>
<p>Lastly, we will show results from oligonucleotides and proteins analyzed on LC/MS single quadrupole and Time of Flight (TOF) instruments, the latest scientific applications, and recent method improvements.</p>
<blockquote>
<p><strong>Presenter:</strong> Ian Edwards, Ph.D. (LC/TQ Portfolio Product Manager, Agilent Technologies Inc.)</p>
</blockquote>
<blockquote>
<p>Ian holds a PhD from the Centre for Proteome Research at the University of Liverpool. He has spent time as a pre- and post-sales MS applications specialist focused on MS imaging and proteomics, as a field marketing manager for -omics, and as a global pharmaceutical marketing manager. Ian joined Agilent recently as LC/TQ Product Manager.</p>
</blockquote>
<blockquote>
<p><strong>Presenter:</strong> Russell Burge, Ph.D. (LC/SQ and LC/TOF Portfolio Product Manager, Agilent Technologies Inc.)</p>
</blockquote>
<blockquote>
<p>Russell earned a PhD in biochemistry and molecular biology from Scripps Research. He has extensive experience in the R&amp;D of biomolecules and biopharmaceuticals, and the implementation of automated workflows. Russell has acted as a Product Manager for various products in Life Science and Industrial Markets, and he currently manages the LC/MS SQ and LC/MS TOF product portfolios at Agilent.</p>
</blockquote>
<blockquote>
<p><strong>Presenter:</strong> Christian Klein, Ph.D. (LC/Q-TOF Portfolio Product Manager, Agilent Technologies Inc.)</p>
</blockquote>
<blockquote>
<p>With an education in Chemistry, Chris Klein received his Ph.D. in 2005 at the Max-Planck-Institute for Biochemistry in Martinsried, Germany. He joined Agilent Technologies in 2010 as the lead chemist in the LC/Q-TOF R&amp;D team. In 2018 Chris jointed the marketing team as Portfolio Product Manager, responsible for LC/Q-TOF and Ion Mobility.</p>
</blockquote>
<blockquote>
<p><strong>Presenter:</strong> Róisín Murtagh (Editor, Wiley)</p>
</blockquote>
<blockquote>
<p>Róisín Murtagh has been an editor for Wiley Analytical Science since 2021. She holds a science degree from Maynooth University, Ireland, and an MSc in Medicinal Chemistry from the Open University, UK. With a background encompassing over 10 years of experience in analytical science, she has actively contributed to both industry and academic research.</p>
</blockquote>


Wiley: Intelligent LC/MS tools for quantitation and characterization of biopharmaceuticals

Intelligent LC/MS tools for quantitation and characterization of biopharmaceuticals

End-to-end solutions for biopharma lab workflows with a focus on multiple mass spectrometry- based analysis techniques, ranging from SQ, TQ, TOF and Q-TOF.**

LC/MS quantitation & characterization of biopharmaceuticals

<p><strong>In this webinar, we will walk you through the use of Zeno SWATH DIA on the ZenoTOF 7600 system for high-performance proteomics analysis, highlighting the following:</strong></p>
<ul>
<li><p>Maximizing nanoflow chromatographic separation performance using IonOpticks Aurora SX series columns</p>
</li>
<li><p>Achieving high sensitivity for protein detection and quantitation on low column loads of a complex digest standard, approximating single-cell protein levels</p>
</li>
<li><p>Demonstrating depth of proteome coverage and quantitative fidelity with mixtures of digests from 3 different organisms, with protein ratios between mixtures accurately quantified using a label-free quantitation (LFQ) strategy</p>
</li>
</ul>
<blockquote>
<p><strong>Presenter:</strong> Patrick Pribil (SCIEX)</p>
</blockquote>


SCIEX: Pushing the boundaries of depth-of-coverage for nanoflow proteomics using Zeno SWATH DIA and IonOpticks Aurora SX series columns

LCMS webinars focusing on LC/TOF - page 22