Professor Michaela Rumlová, head of a research group of the National Institute of Virology and Bacteriology at UCT Prague (and Vice-Rector for doctoral studies), holder of a licensed patent &nbsp;for antiretroviral testing assays, and recipient of the Czech Science Foundation’s President’s Award for HIV research, has also become the principal investigator of the bilateral INTER-EXCELLENCE II project “Cellular factors regulating the export and packaging of unspliced ​​retroviral RNA”, starting this year. In this interview, she explains how retroviruses such as HIV “hack” the cell in order to export their own genome from the nucleus into the cytoplasm, and then to the site where it is specifically packaged into nascent viral particles, a key step in viral replication. Her team is collaborating with the NIH (National Institutes of Health) and Rockefeller University to elucidate mechanisms that may help reveal new vulnerable points in the retrovirus replication cycle while simultaneously clarifying related cellular processes.<h5>Could you describe your project and its main objectives?</h5>Retroviruses, such as HIV, are viruses that carry genetic information in the form of RNA; however, after entering a cell, they are able to reverse transcribe it into DNA and integrate it into the host genome. In order to replicate, they must transport their genetic information—in the form of unspliced RNA—from the nucleus into the cytosol. There, it serves both as a template for the production of viral proteins and as the genome of new viral particles. This is highly unusual, as the cell very carefully controls which RNA molecules are allowed to leave the nucleus. Normal protein-coding cellular RNAs, known as messenger RNAs (mRNAs), must undergo splicing—a processing step in which certain segments are removed—before being exported. When this process fails, mRNA that retains these segments is held back in the nucleus as part of quality control and subsequently degraded. Retroviruses have managed to outwit this cellular control machinery, allowing their unspliced RNA to pass into the cytoplasm.Our project focuses on identifying the cellular proteins that assist retroviruses in this step and on the mechanism by which protein complexes bound to viral RNA are remodelled during RNA transport. The goal is to understand how the virus uses cellular mechanisms to its advantage and how this pathway is linked to the subsequent fate of the RNA, i.e. to its translation and packaging into new viral particles.<h5>Why is this export of unspliced ​​RNA so important for retroviral replication?</h5>Because this RNA has a dual role for the virus. On the one hand, it serves as a template for synthesizing viral proteins in the cytoplasm; on the other, it represents the genetic material itself, which must be packaged into new viral particles. The virus must package its RNA genome in its unspliced form, as only this RNA contains essential sequences without which further viral replication would be impossible.Simply put: the virus needs to get a complete copy of its genetic instructions out of the nucleus. Once the viral RNA is in the cytoplasm, it can be used as a template for synthesizing viral proteins; and a portion of these RNA molecules is then selected and packaged into a new particle as a genome. We are interested in how the virus forces the cell to allow viral RNA that is, from the cell’s perspective, “unfinished,” that is unspliced, to leave the nucleus. We are also investigating how the cell and the virus together - albeit unintentionally from the cell’s perspective - determine which RNA molecule will be translated into proteins and which will be packaged as the genome. This is one of the key, yet still not fully understood, steps of retroviral replication.<h5>What do you think it was about your project that impressed the Ministry of Education, Youth and Sports’ jury the most?</h5>I believe the decisive factor was a combination of several elements. First, the reviewers were primarily interested in the scientific problem itself. We are investigating a mechanism that is essential for retroviruses, but still not sufficiently understood, which may also have broader implications for the regulation of cellular processes. In addition, the quality of the team, our strong collaboration with the NIH and Rockefeller University, and the use of modern methods that will allow us to track viral RNA in great detail also played a role. It was also important that the project has clear objectives and a realistic plan.<h5>How will this research contribute to the development of new treatments for retroviral infections?</h5>It is important to say that this is primarily basic research. Rather than developing a new drug directly, we are trying to understand, at the molecular level, the step that the virus cannot do without. Only when we fully understand the mechanism of these cellular processes and identify the essential factors for the export of unspliced ​​RNA and how they function, will we be able to better determine which interactions or mechanisms represent potentially vulnerable points. Similar mechanisms associated with the export of unspliced ​​mRNA also appear in some diseases, such as cancer, so I &nbsp;see the benefit of our research mainly in that it will refine our understanding of these pathological processes in the cell, and retroviruses help us do so. Therefore, our work on retroviruses not only shows us how the virus works, but also how the cell itself works.<h5>What responsibilities does your UCT Prague team have? How does the collaboration with your American partners work?</h5>At UCT Prague, we are conducting the project’s primary experimental work. Our research involves cell cultures, viral models, and various biochemical techniques ranging from immunochemistry to microscopy that allow us to track viral RNA and identify its cellular partners. In collaboration with colleagues from NIH, Frederick, we are developing single-particle tracking methods to observe viral RNA movement in living cells across both space and time. Furthermore, we are working with colleagues from Rockefeller University to employ iCLIP methods for the precise mapping of protein-binding sites on RNA. Both collaborations are valuable to us, because they are not just about data sharing, but also about the transfer of expertise and detailed know-how.<h5>Who else is collaborating on the project?</h5>At UCT Prague, we collaborate closely with the Department of Biotechnology and the Department of Biochemistry and Microbiology. Externally, we work with colleagues from the Institute of Organic Chemistry and Biochemistry of the CAS (IOCB Prague) and the Central European Institute of Technology in Brno (CEITEC). Our research naturally necessitates the integration of diverse fields of expertise; it requires combining virology, cell biology, and biochemistry with advanced imaging and modern approaches to study RNA-protein interactions. This is exactly what makes our project capable of addressing complex questions that a single laboratory cannot tackle on its own.<h5>Are there plans to continue the project? Have you brought on new collaborators, and are you looking to welcome new colleagues to the team?</h5>Yes, definitely. In basic research, it often happens that one answered question opens up several more. High-quality, properly interpreted data provide a foundation you can build on — and that is what moves scientists forward. It is already clear that the topic of retroviral RNA export and packaging has broader implications and may reveal more general principles of how RNA functions within the cell. We also hope the project will be attractive to students, as it connects classical molecular biology, biochemistry, and virology with cutting-edge biotechnological methods. Students are already involved in the project as part of their bachelor’s and master’s theses.<h5>What was the personal turning point that motivated you to pursue a long-term investigation into retroviral particles?</h5>I first encountered retroviruses in the early 1990s during my diploma thesis, when I was working at IOCB Prague on HIV-1 protease inhibitors. At that time, AIDS could not yet be effectively treated, and retrovirus research had a completely different dimension. From the very beginning, I was fascinated by how such a relatively small and simple virus can outsmart evolutionarily highly optimized cellular processes and turn them to its own advantage. And that has never stopped fascinating me. From a scientific point of view, retroviruses are remarkable; just consider one of their enzymes, reverse transcriptase, without which no diagnostic or research laboratory in the world could function today. Without it, it would be impossible to diagnose RNA viruses like SARS-CoV-2, influenza, or HIV using RT-PCR tests, or, for example to produce recombinant human proteins in bacterial cells.

Tracking unspliced RNA: Professor Rumlová studies how retroviruses “hack” cells

News from LabRulezLCMS Library - Week 20, 2026

🎤Lindsay Repka&nbsp;Lindsay Repka is a chemistry professor at Middlebury College whose research explores photoredox chemistry, catalyst stability, and visible-light-driven transformations. Her lab emphasizes both mechanistic insight and hands-on student training in advanced analytical instrumentation.In this episode of Concentrating on Chromatography, we sit down with Lindsay Repka to discuss how LC-MS and GC-MS transformed her lab’s approach to photoredox chemistry.What began as a project to develop a visible-light photocrosslinking handle unexpectedly led to a major discovery: the solvent (DMF) was reacting with the photocatalyst itself. Using high-resolution LC-MS, Lindsay’s team observed multiple solvent adducts forming — sometimes with complete catalyst consumption. That discovery reshaped their research direction.This episode is a rare deep dive into both LC-MS and GC-MS within the same research project, showing how chromatography-driven insight can turn unexpected degradation into productive new reactivity.If you work in:<ul><li>Photoredox chemistry</li><li>Reaction optimization</li><li>Mass spectrometry method development</li><li>Catalyst screening</li><li>Academic synthetic chemistry</li></ul>…this conversation will resonate.<h4>Key Topics Covered</h4><ul><li>Photocatalyst stability in DMF, DCE, and MeCN</li><li>Demethylation under mild visible-light conditions</li><li>High-resolution Q-TOF LC-MS quantitation</li><li>Internal standard methodology in GC-MS</li><li>Signal-to-noise improvement using extracted ion chromatograms</li><li>Reaction reproducibility and quality control strategy</li></ul><h3>Video Transcription</h3>In this interview, Lindsay Repka discusses the role of photoredox chemistry in method development, photocrosslinking, solvent activation, catalyst stability, and the use of LC-MS and GC-MS to understand reaction pathways.<h4>What is photoredox chemistry?</h4>Photoredox chemistry uses light to activate a photocatalyst, which can either donate or accept an electron from a substrate. This generates radical species that can undergo new types of reactivity.Repka explains that one of the key advantages of photoredox chemistry is its ability to operate under mild conditions, often using visible light. This makes it attractive for sensitive molecules and biological systems, including potential applications in drug release, live-cell chemistry, and drug development.<h4>Photocrosslinking and drug-target interactions</h4>Repka’s group initially aimed to develop visible-light photocrosslinking methods to study drug interactions with biological targets. Photocrosslinking can covalently link a drug to its target, making it easier to identify the biological target of a compound.Traditional photocrosslinking often uses UV light, but visible light offers milder and more biologically compatible conditions.<h4>From photocrosslinking to solvent activation</h4>The project shifted direction when the desired photocrosslinking reactions gave very low yields. Instead of reacting with amino acids as intended, the radical intermediate mainly reacted with itself.The team explored different solvents to stabilize the radical, but found that some solvents, such as DMF, were not inert. Instead, the solvent reacted with the photocatalyst or radical species, revealing unexpected solvent activation chemistry.<h4>Why LC-MS was critical</h4>LC-MS became essential because the reaction mixtures were too complex and low-yielding for NMR or GC-MS. High-resolution LC-MS allowed the team to detect minor products, identify photocatalyst modifications, and observe solvent addition products with high confidence.Repka emphasizes that LC-MS was both a qualitative and quantitative tool. It was used quantitatively to measure how much photocatalyst remained after light exposure, and qualitatively to identify degradation products and new reactivity pathways.<h4>Photocatalyst stability workflow</h4>The team developed a systematic workflow to evaluate photocatalyst stability under light exposure. Because catalyst degradation was time-sensitive, reproducibility required strict control of experimental conditions.Key steps included using a glove box, commercial deoxygenated solvents, accurate weighing on a microbalance, controlled light exposure in a photoreactor, and temperature control with a chiller. These precautions helped reduce day-to-day variability and enabled reproducible measurements of catalyst remaining.<h4>Quantifying catalyst remaining by LC-MS</h4>Calibration curves were prepared using pure photocatalyst solutions and LC-MS ion areas. The team worked in the nanomolar range to maintain linearity.Instead of relying only on total ion chromatograms, they used extracted ion chromatograms (EICs) to improve signal-to-noise for the photocatalyst signal. Baseline subtraction was also applied to reduce noise and improve quantitation at low concentrations.Calibration curves and quality control samples were prepared and measured on the same day as the reaction samples, because LC-MS signal intensity can vary significantly from day to day.<h4>Why GC-MS was used later</h4>For demethylation studies, the group moved to GC-MS because the substrates and products were small and volatile enough for gas chromatography. GC-MS was better suited for rapidly screening many reaction conditions, including different photocatalysts, solvents, and oxidants.Instead of generating full calibration curves for every experiment, the team used an internal standard to streamline analysis and determine percent conversion based on relative response factors.<h4>Practical lessons from the project</h4>Repka highlights the importance of careful sample preparation, especially when working with very small quantities of photocatalysts. A high-quality microbalance, stable granite platform, antistatic tools, and aluminum weigh boats improved weighing accuracy and reproducibility.She also argues that mass spectrometry should be a standard part of photoredox method development because it can reveal minor products, support reaction optimization, and provide insights that may be difficult or impossible to obtain by NMR alone.<h4>Final perspective</h4>Beyond the chemistry, Repka emphasizes the importance of student training and mentoring. For her, research is not only about discovering new reactivity but also about giving students practical experience, helping them develop independence, and preparing them for future scientific careers.<blockquote>This text has been automatically transcribed from a video presentation using AI technology. It may contain inaccuracies and is not guaranteed to be 100% correct.</blockquote><blockquote><h4>Concentrating on Chromatography Podcast</h4>Dive into the frontiers of chromatography, mass spectrometry, and sample preparation with host David Oliva. Each episode features candid conversations with leading researchers, industry innovators, and passionate scientists who are shaping the future of analytical chemistry. From decoding PFAS detection challenges to exploring the latest in AI-assisted liquid chromatography, this show uncovers practical workflows, sustainability breakthroughs, and the real-world impact of separation science. Whether you’re a chromatographer, lab professional, or researcher you'll discover inspiring content!You can find Concentrating on Chromatography Podcast in podcast apps:<ul><li><a target="_blank" rel="noopener noreferrer" href="https://open.spotify.com/show/1wZg67rCjoGju4yhqxI3tL">Spotify</a></li><li><a target="_blank" rel="noopener noreferrer" href="https://podcasts.apple.com/tz/podcast/concentrating-on-chromatography/id1788429185">Apple podcast</a></li><li><a target="_blank" rel="noopener noreferrer" href="https://podscan.fm/podcasts/concentrating-on-chromatography">podscan</a></li></ul>and on <a target="_blank" rel="noopener noreferrer" href="https://www.youtube.com/@ChromatographyTalk/videos">YouTube channel</a></blockquote>

Using LC-MS & GC-MS to Decode Photoredox Catalyst Stability

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The University of Chemistry and Technology, Prague is a natural centre of first-rate study and research in the area of chemistry in Czechia and is one of the country's largest educational and research institutions focused on technical chemistry, chemical and biochemical technologies, material and chemical engineering, food chemistry, and environmental studies.

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Waters Corporation

<h3>Solving Problems That Matter - a Waters webinar series featuring USP speakers</h3>
<h3>Episode 4: aQbD for OTC products</h3>
Summary
Analyzing over-the-counter formulations can be overwhelming due to the wide range of APIs and excipients used in varying concentrations. These excipients can cause problematic matrix effects and coelutions, making the analysis of formulations complex. Our panelists will discuss how to leverage aQbD software to develop a single chromatographic method suitable to analyze multiple APIs in a variety of formulations.
Key learning objective:
Learn how to leverage aQbD tools to develop a single method suitable for a variety of OTC formulations.
Who sould attend:
Pharmaceutical method developers 
<blockquote>
Presenter: Fadi Alkhateeb (Principle Scientist, Waters Corporations)
</blockquote>
<blockquote>
Dr. Fadi Alkhateeb is a Senior Scientist in the Scientific Operations Department at WatersCorporation HQ in Milford, MA USA. He joined Waters in 2017 and since then he has beenmainly focusing on exploring various approaches for analytical method development forpharmaceutical drugs. Prior to joining Waters, he was a postdoctoral fellow at the University ofCalgary (Calgary, AB, Canada), where he did research at Alberta Sulphur Research, one ofNorth America&#39;s leading Sulphur research institutes. He received his PhD from the University ofCalgary, focusing on the development of novel, rapid separation and sample preparationtechniques for the analysis of a wide range of chemical compounds. Dr. Alkhateeb publishedseveral peer-reviewed articles in top analytical journals.​
</blockquote>
<blockquote>
Moderated by: Isabelle Vu Trieu (Pharmaceutical Market Development Manager, Americas, Waters Corporation)
</blockquote>
<blockquote>
Isabelle is a business development professional dedicated to delivering benefits to analytical scientists in the life sciences industry. With over 20 years of experience in providing chromatography and mass spectrometry solutions, Isabelle is particularly interested in helping pharmaceutical scientists achieve the data quality and integrity needed to drive decisions for drug quality, safety and efficacy. Isabelle joined Waters in 2009 and currently manages the development of the pharmaceutical market in the Americas. She holds Masters’ degrees in Chemistry from Columbia University and the University Claude Bernard in Lyon, France as well as a Chemical Engineering degree from Ecole Supérieure de Chimie Physique Electronique in Lyon, France.
</blockquote>

Waters Corporation: Solving Problems That Matter - a Waters webinar series featuring USP speakers

aQbD for OTC products

Solving Problems That Matter - a Waters webinar series featuring USP speakers. Episode 4: aQbD for OTC products.

PhenoAcademy Webinars
 Liquid Chromatography Series
<h4>HPLC Troubleshooting</h4>
<ul>
<li>Learn how to prevent and minimize your LC problems before they occur</li>
<li>Understand how to approach problem solving and troubleshooting in a logical way, including: diagnose cause and possible solutions</li>
<li>Review peak shape, and column performance problems</li>
</ul>
<blockquote>
Presenter: Kevin Zhao (Senior Technical Specialist)
</blockquote>
<blockquote>
Kevin Zhao is the Senior Technical Specialist with Phenomenex and has 5 years of experience in biochemistry research; 4 years of Protein/Cancer research with CSULA-City of Hope Cancer Collaborative and over 9 years in Phenomenex. Mr. Zhao is a member of a global Technical Support Team which includes providing problem-solving, troubleshooting, and applications assistance for customers worldwide in the fields of chromatography (liquid and gas) and sample preparation, and also handling Industries supported include – pharmaceutical and biopharmaceutical, clinical research and clinical toxicology, food safety, environmental, fuels and specialty chemicals. Mr. Zhao received his B.S. in Biology at the California State Polytechnic University, Pomona. earned his M.S. in Biochemistry at California State University, Los Angeles, and also earned a MBA at the University of the Incarnate Word.
</blockquote>
<blockquote>
Presenter: Koen Askamp (Technical Specialist)
</blockquote>
<blockquote>
Koen is from the Netherlands, studied Molecular Life Sciences at Wageningen University (Netherlands), majoring in immunology. Analytical Chemistry was one of my favorite courses – who doesn’t love solving puzzles?
</blockquote>
<blockquote>
I like drumming, scuba diving, motorcycle-riding, and this past winter I learned how to ski (a little)!
</blockquote>
<blockquote>
Presenter: Namrata Saxena (Technical Manager)
</blockquote>
<blockquote>
Namrata Saxena is a Technical Manager - Asia/Pacific” with Phenomenex and is based in Sydney, Australia. She has a post graduate degree with a specialization in Analytical Chemistry, with extensive experience in the Liquid chromatography domain, and her current focus is in Omics and Biologics field. 
</blockquote>
<blockquote>
Before joining Phenomenex she had worked as “Product Specialist” and spent several years in column chemistry, column manufacturing and method development in the application and manufacturing labs of Bischoff Chromatography, Germany. 
</blockquote>
<blockquote>
In her current role, she provides product selection, method development, training and troubleshooting support to customers as well as Phenomenex Sales Consultants globally. 
</blockquote>

Phenomenex: PhenoAcademy Webinars - Liquid Chromatography Series

HPLC Troubleshooting

Learn how to prevent and minimize your LC problems before they occur.

<h4>Facing Challenges in Your Liquid Chromatography Workflow?</h4>
How do you tackle peak shape issues, pressure fluctuations, or retention time inconsistencies? Where do you turn for insights on method optimization or lab workflow management? If these challenges sound familiar, our upcoming session is your opportunity to find answers.
This exclusive session, led by a panel of seasoned Agilent LC experts, is designed to address the hurdles you face in your LC workflows. Get actionable insights, practical tips, and proven strategies to not only overcome these obstacles but to significantly enhance your workflow&#39;s efficiency. 
No challenge is too big or small for our discussion. Our goal is to give you the answers you need. 
When registering, you&#39;re invited to submit your burning questions and specific challenges, ensuring that our panel addresses what matters the most to you and provides tailored solutions. 
Why Attend?
<ul>
<li>Engage with Experts: Your chance to ask questions and get answers from leading Agilent LC experts</li>
<li>Exclusive Rewards: Receive a special promo code for LC Columns, a certificate of attendance, and an LC Workflow Resource Kit, filled with tools, guides, and application notes</li>
<li>Professional Growth: This session isn&#39;t just about solving today&#39;s problems; it&#39;s about equipping you for tomorrow&#39;s successes, reaffirming your commitment to staying at the forefront of analytical science</li>
</ul>
Don’t let workflow challenges slow you down.
<blockquote>
Presenter: Mr. Sunil B Lakhmapure (Technical Support Specialist, Agilent Technologies)
</blockquote>
<blockquote>
Sunil holds 19 year plus experience in the analytical field with major exposure to LC/GC with Single Quad, Triple Quad and high resolution (QToF) mass spectroscopy. Started career in Pharma R&amp;D as a scientist with hands on experience on high end instruments like LC/MS, XRD &amp; NMR. Developed &amp; published application notes using Chromatographic techniques on real world samples/issues at various levels.
</blockquote>
<blockquote>
Presenter: Ms. Sreelakshmy Menon (Application Scientist, Agilent Technologies)
</blockquote>
<blockquote>
Sreelakshmy is working as an Application Specialist, at Agilent Technologies for the past 11 years. She has experience in working with Analytical techniques such as HPLC, UHPLC, CE, SFC, Method development solutions etc. Her primary interest is in developing liquid chromatography application solutions focusing on AQbD approach concentrating on pharma and biopharma workflows. To her credit she holds around12 publications on innovative Liquid Chromatographic solutions.
</blockquote>
<blockquote>
Presenter: Mr. Pintu Nandasana (Application Engineer, Solution Support Division, Agilent Techologies India)
</blockquote>
<blockquote>
Pintu Nandasana is working as Application Engineer at Agilent Technologies India from last 11 years. Education background, He has completed master’s in pharmacy. Currently as a Field System Specialist, he is supporting all PL29 products like LC, LCMS, GPC, 2D-LC, Preparative LC &amp; Method development solutions.
</blockquote>
<blockquote>
Presenter: Mr. Shreyas Tribhuvane (Business Development Manager, LC Columns, Agilent Technologies)
</blockquote>
<blockquote>
Shreyas has 10 years experience in working closely with biopharma customers in understanding their analytical characterization needs. As a BDM for Biocolumns and Glycan kits his prime responsibility is to work on the Biopharma market needs and communicate with Agilent solutions unit team in order to have apt solutions. He has a hands-on experience on Agilent Glycan sample prep kits and helped customers in setting up these kits for their glycan characterization work.
</blockquote>

Agilent: Decoding LC: Navigating Workflow Challenges

Decoding LC: Navigating Workflow Challenges

Insights, tips, and proven strategies to not only overcome obstacles but to significantly enhance your workflow's efficiency.

<h4>LC-MS/MS fundamentals webinar series 2.0</h4>
Advanced techniques for data acquisition and processing
Elevate your analytical expertise with advanced techniques and workflows
Sharpen your data acquisition and processing skills with our LC-MS/MS 2.0 webinar series. Based on our LC-MS/MS 1.0 webinar series, these sessions will go beyond the fundamentals of LC-MS/MS and introduce ways to advance your analytical methods and data processing workflows.
Every other week, we will explore how to optimize SCIEX OS software with short, 30-minute expert-led presentations.
<h4>This webinar:</h4>
June 4, 2024 11:00 a.m. - 11:30 a.m. CET - Mastering chromatography
Discover how to optimize your chromatography for even the most challenging compounds while creating and building LC methods.
<h4>Other webinars from the series:</h4>
May 7, 2024 11:00 a.m. - 11:30 a.m. CET - <a href="https://lcms.cz/webinar/3820">Beyond the fundamentals</a>
May 21, 2024 11:00 a.m. - 11:30 a.m. CET - <a href="https://lcms.labrulez.com/webinar/3852">Advanced MS/MS method building</a>
Gain insight into advanced features for maximizing sensitivity in your mass spectrometry analyses.
June 18, 2024 11:00 a.m. - 11:30 a.m. CET - <a href="https://lcms.labrulez.com/webinar/3854">It&#39;s a TRAP! Getting the most out of your QTRAP system</a>
Learn when to utilize QTRAP functionality to create more selective and sensitive methods.
July 2, 2024 11:00 a.m. - 11:30 a.m. CET - <a href="https://lcms.labrulez.com/webinar/3855">Troubleshooting at the source</a>
Acquire the knowledge needed to optimize source parameters and identify and resolve ionization-related problems.
July 16, 2024 11:00 a.m. - 11:30 a.m. CET - <a href="https://lcms.labrulez.com/webinar/3856">Automating data processing</a>
Learn how to automate your data processing with custom flagging and rules for efficient data review in SCIEX OS software.
July 30, 2024 11:00 a.m. - 11:30 a.m. CET - <a href="https://lcms.labrulez.com/webinar/3857">Excel strategies for mass spectrometry data analysis</a> Take an in-depth look at when and how to use Excel to increase your productivity.

SCIEX: LC-MS/MS fundamentals webinar series 2.0

Mastering chromatography

Sharpen your data acquisition and processing skills with our LC-MS/MS 2.0 webinar series.

The successful regulatory filing of any pharmaceutical product development dossier relies on scientific and rational way of analytical method development.
There are many analytical development strategies which can be followed like “Traditional” or “Enhanced approach”. Enhanced Approach as stated by ICH, or DoE based Quality-by-Design (QbD) approach are one of such method development strategies which will be discussed in webinar. These approaches are now part of regulations across USP, ICH and USFDA. We will also discuss the different concepts like ATP, Lifecycle Management, Risk Assessment etc. Join us for this webinar to understand different guidelines related to analytical development and how can you adopt QbD or Enhanced approach in the analytical method development.
<blockquote>
Presenter: Manu Grover (Business Development Manager – Pharma Markets, Agilent Technologies, Inc.)
</blockquote>
<blockquote>
Manu Grover holds a postgraduate degree in Pharmacy from Manipal Academy, India. He has more than 21 years of chromatography experience and has worked in pharmaceuticals to support IND, NDA, and ANDA filings incompanies such as Dr. Reddy&#39;s, Panacea Biotec, and Ozone Pharmaceuticals. During his professional journey, he has developed and validated analytical methods on HPLC, GC, particle sizing, DSC, pXRD, and LC/MS instruments, as well as successfully filing multiple analytical dossiers in U.S./EU markets. In his current role, he works closely with the Agilent sales team, regulators, and pharmaceutical scientists. He shares market updates, trends, and expertise related tothe Agilent portfolio of columns and supplies.
</blockquote>

Agilent: Becoming a Better Chromatographer - Educational Webinar Series

Regulatory Guidelines on Analytical Method Development: USFDA, ICH and USP Perspective

Understand different guidelines related to analytical development and how can you adopt QbD or Enhanced approach in the analytical method development.

Regulatory Guidelines on Analytical Method Development

HPLC is a powerful analytical technique widely used in the food industry, research labs and commercial testing labs for quality control, safety assessment, and nutritional analysis. It is particularly valuable for analyzing compounds such as vitamins, amino acids, sugars, oils and toxins like mycotoxins. HPLC plays a crucial role in ensuring the safety, quality, and nutritional integrity of food products. Its ability to provide detailed information about the composition of food samples makes it an indispensable tool for food testing laboratories and the food industry.
Altium is happy to announce webinar on Agilent LC solutions for food industry. Reserve your spot now!
<a href="https://events.teams.microsoft.com/event/ecf82ef6-1be9-404d-8dda-732d993c8699@3a2a17c9-a748-438b-8c0e-b375b3713c75">REGISTRATION</a>
Key learning points:
<ul>
<li>HPLC introduction.</li>
<li>Key HPLC features which can reduce human error and enhance efficiency.</li>
<li>HPLC applications for vitamins analysis.</li>
<li>HPLC applications for amino acid analysis using automated pre column derivatization method.</li>
<li>HPLC application for sugar analysis.</li>
<li>HPLC application for aflatoxin analysis.</li>
<li>GPC as a technique of sample prep for removing high molecular matrix.</li>
</ul>
There will be a Q&amp;A session with the support of local Altium teams.
Looking forward to meeting you.
<blockquote>
Speaker: Laura Montis (IDO Product Specialist LPS Agilent Technologies)
</blockquote>

Altium International: Agilent LC Solutions For Food Testing and Food Fortification Webinar

Agilent LC Solutions For Food Testing and Food Fortification Webinar

HPLC is widely used in the food industry, research labs and commercial testing labs for quality control, safety assessment, and nutritional analysis.

Agilent LC Solutions For Food Testing and Food Fortification

<h4>Waters 2024 LC Symposium</h4>
Pushing the Boundaries of LC to Address Today’s Challenges
<h5>Overview</h5>
Today’s scientists and lab managers need to confidently deliver accurate, reliable, and reproducible data while facing increasingly complex molecules, aggressive sustainability goals, and accelerated timelines. 
Join this free symposium to get insights from global LC thought leaders on the advancements of LC technology to address current challenges. 
<h3>Day 1 – June 12 (10:00 AM EDT | 15:00 PM BST | 16:00 PM CEST)</h3>
<h5>Advanced LC Technologies for Increasingly Complex Molecules</h5>
Our panel of industry leaders will discuss recent developments in liquid chromatography designed to meet today&#39;s analytical challenges. Our speakers will explore innovative technologies to mitigate unpredictable chromatography due to non-specific adsorption and explore the insights from light scattering detection.
<h5>Learning objectives</h5>
<ul>
<li>Hear from LC thought leaders about state-of-the-art LC technologies </li>
<li>Learn how to control metal-based adsorption</li>
<li>Explore orthogonal detection schemes</li>
<li>Discover the next generation of intuitively simple LC systems</li>
</ul>
Join us for Waters 2024 LC Symposium, we are really looking forward to answering your questions in our live Q&amp;A session and discussing this topic further with you.
<blockquote>
<h3>Day 2 – June 13 (10:00 AM EDT | 15:00 PM BST | 16:00 PM CEST)</h3>
</blockquote>
<blockquote>
<h5>Next Generation HPLC: the Quest for Performance and Simplicity</h5>
</blockquote>
<blockquote>
Advancements in HPLC technology impact routine testing laboratories where error reduction, ease of use and system performance play a crucial role. Our expert panel will discuss recent advances in HPLC technology, revisions to the USP &lt;621&gt; guidelines, application of Analytical Quality by Design (AQbD) software to assess method robustness, and innovative technologies which enhance simplicity and performance.
</blockquote>
<blockquote>
Presenter: Davy Guillarme, Ph.D. (Sr Lecturer and Research Associate, University of Geneva)
</blockquote>
<blockquote>
Davy Guillarme, holds a Ph.D. in analytical chemistry from the University of Lyon, France, currently serves as a senior lecturer and research associate at the University of Geneva in Switzerland. With over 340 published journal articles in pharmaceutical analysis and h-index of 75, his expertise spans various techniques, including HPLC, UHPLC, HILIC, LC−MS, SFC, SFC-MS, multidimensional LC, and the analysis of biopharmaceuticals (proteins, mAbs, oligonucleotides, gene therapy products, mRNA…).
</blockquote>
<blockquote>
He is an associate editor of the Journal of Chromatography A and has been honored with the LC-GC Emerging Leader Award in Chromatography (2013), the Jubilee Medal from the Chromatographic Society (2018), and the International Award of the Belgian Society of Pharmaceutical Sciences (2022). His global recognition extends to being listed among the world&#39;s most influential analytical scientists by &quot;Analytical Scientist&quot; magazine in multiple years.
</blockquote>
<blockquote>
Presenter: Guilherme (Gui) Guimaraes, Ph.D. (Scientist, Biogen)
</blockquote>
<blockquote>
Dr. Guilherme (Gui) Guimaraes is a scientist in drug metabolism and pharmacokinetics at Biogen. He is responsible for bioanalytical analysis of small molecules and antisense oligonucleotides. Gui holds a Ph.D in pharmaceutical and biomedical sciences and a masters in international biomedical regulatory sciences from the University of Georgia. His graduate work was focused on the application of novel analytical strategies to improve the chromatographic performance and sensitivity of oligonucleotide LC-MS methods. Some of these strategies include the use of low adsorption surfaces and microflow LC-MS.
</blockquote>
<blockquote>
Presenter: Michelle Chen, Ph.D. (Sr Director, Research and Development, Waters l Wyatt Technology)
</blockquote>
<blockquote>
Dr. Michelle Chen is a senior director at Waters | Wyatt Technology located in Santa Barbara, California. Michelle obtained her Ph.D. from the Chemical Engineering Department at Yale University, where she focused on developing novel HPLC approaches for high-speed and high-efficiency separations of biopolymers. Since joining Wyatt, Michelle has incorporated multi-angle light scattering and dynamic light scattering detection with HPLC and field-flow fractionation, for characterizing synthetic and biological polymers, proteins, and nanoparticles. In recent years she has led the application team at Wyatt Technology to develop novel methods to characterize and quantify emerging therapeutics and delivery vehicles including new protein conjugates, viral vectors, lipid nanoparticles, and extracellular vesicles.
</blockquote>
<blockquote>
Presenter: Moderated by: Isabelle Vu Trieu (Pharmaceutical Market Development Manager, Americas, Waters Corporation)
</blockquote>
<blockquote>
Isabelle is a business development professional dedicated to delivering benefits to analytical scientists in the life sciences industry. With over 20 years of experience in providing chromatography and mass spectrometry solutions, Isabelle is particularly interested in helping pharmaceutical scientists achieve the data quality and integrity needed to drive decisions for drug quality, safety and efficacy. Isabelle joined Waters in 2009 and currently manages the development of the pharmaceutical market in the Americas. She holds Masters’ degrees in Chemistry from Columbia University and the University Claude Bernard in Lyon, France as well as a Chemical Engineering degree from Ecole Supérieure de Chimie Physique Electronique in Lyon, France.
</blockquote>

Waters Corporation: Waters 2024 LC Symposium

Advanced LC Technologies for Increasingly Complex Molecules

Free symposium to get insights from global LC thought leaders on the advancements of LC technology to address current challenges.

<h4>Waters 2024 LC Symposium</h4>
Pushing the Boundaries of LC to Address Today’s Challenges
<h5>Overview</h5>
Today’s scientists and lab managers need to confidently deliver accurate, reliable, and reproducible data while facing increasingly complex molecules, aggressive sustainability goals, and accelerated timelines. 
Join this free symposium to get insights from global LC thought leaders on the advancements of LC technology to address current challenges. 
<h3>Day 2 – June 13 (10:00 AM EDT | 15:00 PM BST | 16:00 PM CEST)</h3>
<h5>Next Generation HPLC: the Quest for Performance and Simplicity</h5>
Advancements in HPLC technology impact routine testing laboratories where error reduction, ease of use and system performance play a crucial role. Our expert panel will discuss recent advances in HPLC technology, revisions to the USP &lt;621&gt; guidelines, application of Analytical Quality by Design (AQbD) software to assess method robustness, and innovative technologies which enhance simplicity and performance.
Learning objectives
<ul>
<li>Hear from LC thought leaders about state-of-the-art LC technologies </li>
<li>Learn how to control metal-based adsorption</li>
<li>Explore orthogonal detection schemes</li>
<li>Discover the next generation of intuitively simple LC systems</li>
</ul>
Join us for Waters 2024 LC Symposium, we are really looking forward to answering your questions in our live Q&amp;A session and discussing this topic further with you.
<blockquote>
<h3>Day 1 – June 12 (10:00 AM EDT | 15:00 PM BST | 16:00 PM CEST)</h3>
</blockquote>
<blockquote>
<h5>Advanced LC Technologies for Increasingly Complex Molecules</h5>
</blockquote>
<blockquote>
Our panel of industry leaders will discuss recent developments in liquid chromatography designed to meet today&#39;s analytical challenges. Our speakers will explore innovative technologies to mitigate unpredictable chromatography due to non-specific adsorption and explore the insights from light scattering detection.
</blockquote>
<blockquote>
Presenter: Davy Guillarme, Ph.D. (Sr Lecturer and Research Associate, University of Geneva)
</blockquote>
<blockquote>
Davy Guillarme, holds a Ph.D. in analytical chemistry from the University of Lyon, France, currently serves as a senior lecturer and research associate at the University of Geneva in Switzerland. With over 340 published journal articles in pharmaceutical analysis and h-index of 75, his expertise spans various techniques, including HPLC, UHPLC, HILIC, LC−MS, SFC, SFC-MS, multidimensional LC, and the analysis of biopharmaceuticals (proteins, mAbs, oligonucleotides, gene therapy products, mRNA…).
</blockquote>
<blockquote>
He is an associate editor of the Journal of Chromatography A and has been honored with the LC-GC Emerging Leader Award in Chromatography (2013), the Jubilee Medal from the Chromatographic Society (2018), and the International Award of the Belgian Society of Pharmaceutical Sciences (2022). His global recognition extends to being listed among the world&#39;s most influential analytical scientists by &quot;Analytical Scientist&quot; magazine in multiple years.
</blockquote>
<blockquote>
Presenter: Guilherme (Gui) Guimaraes, Ph.D. (Scientist, Biogen)
</blockquote>
<blockquote>
Dr. Guilherme (Gui) Guimaraes is a scientist in drug metabolism and pharmacokinetics at Biogen. He is responsible for bioanalytical analysis of small molecules and antisense oligonucleotides. Gui holds a Ph.D in pharmaceutical and biomedical sciences and a masters in international biomedical regulatory sciences from the University of Georgia. His graduate work was focused on the application of novel analytical strategies to improve the chromatographic performance and sensitivity of oligonucleotide LC-MS methods. Some of these strategies include the use of low adsorption surfaces and microflow LC-MS.
</blockquote>
<blockquote>
Presenter: Michelle Chen, Ph.D. (Sr Director, Research and Development, Waters l Wyatt Technology)
</blockquote>
<blockquote>
Dr. Michelle Chen is a senior director at Waters | Wyatt Technology located in Santa Barbara, California. Michelle obtained her Ph.D. from the Chemical Engineering Department at Yale University, where she focused on developing novel HPLC approaches for high-speed and high-efficiency separations of biopolymers. Since joining Wyatt, Michelle has incorporated multi-angle light scattering and dynamic light scattering detection with HPLC and field-flow fractionation, for characterizing synthetic and biological polymers, proteins, and nanoparticles. In recent years she has led the application team at Wyatt Technology to develop novel methods to characterize and quantify emerging therapeutics and delivery vehicles including new protein conjugates, viral vectors, lipid nanoparticles, and extracellular vesicles.
</blockquote>
<blockquote>
Presenter: Moderated by: Isabelle Vu Trieu (Pharmaceutical Market Development Manager, Americas, Waters Corporation)
</blockquote>
<blockquote>
Isabelle is a business development professional dedicated to delivering benefits to analytical scientists in the life sciences industry. With over 20 years of experience in providing chromatography and mass spectrometry solutions, Isabelle is particularly interested in helping pharmaceutical scientists achieve the data quality and integrity needed to drive decisions for drug quality, safety and efficacy. Isabelle joined Waters in 2009 and currently manages the development of the pharmaceutical market in the Americas. She holds Masters’ degrees in Chemistry from Columbia University and the University Claude Bernard in Lyon, France as well as a Chemical Engineering degree from Ecole Supérieure de Chimie Physique Electronique in Lyon, France.
</blockquote>

Next Generation HPLC: the Quest for Performance and Simplicity

Next Generation HPLC: Performance and Simplicity

This webinar unveils the development of a high-throughput multidimensional liquid chromatography approach in combination with charged aerosol detection (CAD), leveraging a combination of ion exchange and reversed-phase columns, ensuring accurate and precise analysis within minutes.
Discover how this method advances formulation development, stability testing, and batch release, providing researchers with valuable insights into surfactant degradation dynamics. 
Learning Points: 
<ul>
<li>Discover cutting-edge techniques for rapid analysis of surfactants in antibody formulations</li>
<li>Unveil high-throughput liquid chromatography with charged aerosol detection</li>
<li>Gain valuable insights for optimizing formulation development and stability testing</li>
</ul>
Who should attend?
<ul>
<li>Biopharmaceutical scientists and researchers specializing in monoclonal antibody development</li>
<li>Analytical chemists and technicians involved in pharmaceutical formulation analysis</li>
<li>Quality control and regulatory affairs professionals in the pharmaceutical industry</li>
</ul>
<blockquote>
Presenter: Maksymilian Zegota, PhD (Senior Scientist, Coriolis Pharma)
</blockquote>
<blockquote>
Dr. Zegota earned his PH.D. in chemistry from the Max Planck Institute for polymer research in Mainz, Germany, concentrating on site-selective protein modification.
</blockquote>
<blockquote>
After completing his doctorate, he joined Coriolis Pharma in Martinsried, Germany.
</blockquote>
<blockquote>
Currently, Dr. Zegota focuses on developing Novel methods for surfactant analysis in biologics to assist in the root-cause analysis of polysorbate and poloxamer degradation, contributing to formulation development in the pharmaceutical field.
</blockquote>
<blockquote>
Presenter: Marcus Klee (Marcus Klee, Regional Marketing Manager HPLC, Thermo Fisher Scientific)
</blockquote>
<blockquote>
Graduated in Chemical Engineering from Hochschule Fresenius in 2000, specialized in Marketing &amp; Sales. Since then, held various marketing roles since 2000, currently serving as the Regional Marketing Manager EMEA for HPLC
</blockquote>

Thermo Scientific: Advancing analytical techniques in monoclonal antibody formulations

Advancing analytical techniques in monoclonal antibody formulations

Explore cutting-edge methodology for online protein removal and characterization of polysorbates and poloxamer in monoclonal antibody formulations.

Analytical techniques in monoclonal antibody formulations

Advances in monoclonal antibody (mAb) engineering have led to additional challenges in the purification of these modalities, including high levels of aggregates. Ion exchange chromatography is often not sufficient to achieve purity targets meaning hydrophobic interaction chromatography (HIC) and mixed-mode resins are needed as orthogonal tools. These technologies can be implemented in flow-through mode allowing for high loadings and fast processing times, making them well-suited for large-scale implementation.
This webinar highlights three case studies that illustrate how novel chromatographic tools can improve the purification process for complex therapeutics by reducing the levels of aggregates and host cell proteins (HCPs) and effectively clearing retroviruses.
Attend this webinar to explore case studies on:
<ul>
<li>Effective aggregate removal and viral clearance via HIC flow-through unit operation</li>
<li>High molecular weight reduction and viral clearance for a novel, pre-conjugated mAb for ADC production</li>
<li>Simultaneous HCP reduction and aggregate removal with mixed-mode chromatography for a high aggregate mAb feedstream</li>
</ul>
<blockquote>
Presenter: Robert Stairs (Field Applications Staff Scientist, Thermo Fisher Scientific)
</blockquote>

Technology Networks: Advances in Flow-Through Technology To Enhance mAb Polishing

Advances in Flow-Through Technology To Enhance mAb Polishing

Case studies that illustrate how novel chromatographic tools can improve the purification process for complex therapeutics.

LCMS webinars focusing on HPLC - page 24