Professor Michaela Rumlová, head of a research group of the National Institute of Virology and Bacteriology at UCT Prague (and Vice-Rector for doctoral studies), holder of a licensed patent &nbsp;for antiretroviral testing assays, and recipient of the Czech Science Foundation’s President’s Award for HIV research, has also become the principal investigator of the bilateral INTER-EXCELLENCE II project “Cellular factors regulating the export and packaging of unspliced ​​retroviral RNA”, starting this year. In this interview, she explains how retroviruses such as HIV “hack” the cell in order to export their own genome from the nucleus into the cytoplasm, and then to the site where it is specifically packaged into nascent viral particles, a key step in viral replication. Her team is collaborating with the NIH (National Institutes of Health) and Rockefeller University to elucidate mechanisms that may help reveal new vulnerable points in the retrovirus replication cycle while simultaneously clarifying related cellular processes.<h5>Could you describe your project and its main objectives?</h5>Retroviruses, such as HIV, are viruses that carry genetic information in the form of RNA; however, after entering a cell, they are able to reverse transcribe it into DNA and integrate it into the host genome. In order to replicate, they must transport their genetic information—in the form of unspliced RNA—from the nucleus into the cytosol. There, it serves both as a template for the production of viral proteins and as the genome of new viral particles. This is highly unusual, as the cell very carefully controls which RNA molecules are allowed to leave the nucleus. Normal protein-coding cellular RNAs, known as messenger RNAs (mRNAs), must undergo splicing—a processing step in which certain segments are removed—before being exported. When this process fails, mRNA that retains these segments is held back in the nucleus as part of quality control and subsequently degraded. Retroviruses have managed to outwit this cellular control machinery, allowing their unspliced RNA to pass into the cytoplasm.Our project focuses on identifying the cellular proteins that assist retroviruses in this step and on the mechanism by which protein complexes bound to viral RNA are remodelled during RNA transport. The goal is to understand how the virus uses cellular mechanisms to its advantage and how this pathway is linked to the subsequent fate of the RNA, i.e. to its translation and packaging into new viral particles.<h5>Why is this export of unspliced ​​RNA so important for retroviral replication?</h5>Because this RNA has a dual role for the virus. On the one hand, it serves as a template for synthesizing viral proteins in the cytoplasm; on the other, it represents the genetic material itself, which must be packaged into new viral particles. The virus must package its RNA genome in its unspliced form, as only this RNA contains essential sequences without which further viral replication would be impossible.Simply put: the virus needs to get a complete copy of its genetic instructions out of the nucleus. Once the viral RNA is in the cytoplasm, it can be used as a template for synthesizing viral proteins; and a portion of these RNA molecules is then selected and packaged into a new particle as a genome. We are interested in how the virus forces the cell to allow viral RNA that is, from the cell’s perspective, “unfinished,” that is unspliced, to leave the nucleus. We are also investigating how the cell and the virus together - albeit unintentionally from the cell’s perspective - determine which RNA molecule will be translated into proteins and which will be packaged as the genome. This is one of the key, yet still not fully understood, steps of retroviral replication.<h5>What do you think it was about your project that impressed the Ministry of Education, Youth and Sports’ jury the most?</h5>I believe the decisive factor was a combination of several elements. First, the reviewers were primarily interested in the scientific problem itself. We are investigating a mechanism that is essential for retroviruses, but still not sufficiently understood, which may also have broader implications for the regulation of cellular processes. In addition, the quality of the team, our strong collaboration with the NIH and Rockefeller University, and the use of modern methods that will allow us to track viral RNA in great detail also played a role. It was also important that the project has clear objectives and a realistic plan.<h5>How will this research contribute to the development of new treatments for retroviral infections?</h5>It is important to say that this is primarily basic research. Rather than developing a new drug directly, we are trying to understand, at the molecular level, the step that the virus cannot do without. Only when we fully understand the mechanism of these cellular processes and identify the essential factors for the export of unspliced ​​RNA and how they function, will we be able to better determine which interactions or mechanisms represent potentially vulnerable points. Similar mechanisms associated with the export of unspliced ​​mRNA also appear in some diseases, such as cancer, so I &nbsp;see the benefit of our research mainly in that it will refine our understanding of these pathological processes in the cell, and retroviruses help us do so. Therefore, our work on retroviruses not only shows us how the virus works, but also how the cell itself works.<h5>What responsibilities does your UCT Prague team have? How does the collaboration with your American partners work?</h5>At UCT Prague, we are conducting the project’s primary experimental work. Our research involves cell cultures, viral models, and various biochemical techniques ranging from immunochemistry to microscopy that allow us to track viral RNA and identify its cellular partners. In collaboration with colleagues from NIH, Frederick, we are developing single-particle tracking methods to observe viral RNA movement in living cells across both space and time. Furthermore, we are working with colleagues from Rockefeller University to employ iCLIP methods for the precise mapping of protein-binding sites on RNA. Both collaborations are valuable to us, because they are not just about data sharing, but also about the transfer of expertise and detailed know-how.<h5>Who else is collaborating on the project?</h5>At UCT Prague, we collaborate closely with the Department of Biotechnology and the Department of Biochemistry and Microbiology. Externally, we work with colleagues from the Institute of Organic Chemistry and Biochemistry of the CAS (IOCB Prague) and the Central European Institute of Technology in Brno (CEITEC). Our research naturally necessitates the integration of diverse fields of expertise; it requires combining virology, cell biology, and biochemistry with advanced imaging and modern approaches to study RNA-protein interactions. This is exactly what makes our project capable of addressing complex questions that a single laboratory cannot tackle on its own.<h5>Are there plans to continue the project? Have you brought on new collaborators, and are you looking to welcome new colleagues to the team?</h5>Yes, definitely. In basic research, it often happens that one answered question opens up several more. High-quality, properly interpreted data provide a foundation you can build on — and that is what moves scientists forward. It is already clear that the topic of retroviral RNA export and packaging has broader implications and may reveal more general principles of how RNA functions within the cell. We also hope the project will be attractive to students, as it connects classical molecular biology, biochemistry, and virology with cutting-edge biotechnological methods. Students are already involved in the project as part of their bachelor’s and master’s theses.<h5>What was the personal turning point that motivated you to pursue a long-term investigation into retroviral particles?</h5>I first encountered retroviruses in the early 1990s during my diploma thesis, when I was working at IOCB Prague on HIV-1 protease inhibitors. At that time, AIDS could not yet be effectively treated, and retrovirus research had a completely different dimension. From the very beginning, I was fascinated by how such a relatively small and simple virus can outsmart evolutionarily highly optimized cellular processes and turn them to its own advantage. And that has never stopped fascinating me. From a scientific point of view, retroviruses are remarkable; just consider one of their enzymes, reverse transcriptase, without which no diagnostic or research laboratory in the world could function today. Without it, it would be impossible to diagnose RNA viruses like SARS-CoV-2, influenza, or HIV using RT-PCR tests, or, for example to produce recombinant human proteins in bacterial cells.

Tracking unspliced RNA: Professor Rumlová studies how retroviruses “hack” cells

High-performance liquid chromatography (HPLC) remains one of the most widely used analytical techniques for separating, identifying, and quantifying compounds in complex mixtures. Successful HPLC method development depends not only on selecting the correct column and optimizing the mobile phase composition, but also on choosing the appropriate detector. Even a perfectly optimized chromatographic separation provides little value if the detection system cannot properly capture and interpret the analytes of interest.In many ways, the detector acts as the “vision system” of the chromatograph. It converts separated compounds into measurable electronic signals, allowing analysts to identify components and determine their concentrations. The choice of detector therefore has a major impact on sensitivity, selectivity, robustness, and the overall quality of analytical data.This article provides an overview of the most common HPLC detection techniques, discusses their strengths and limitations, and outlines the most important criteria to consider when selecting a detector for a specific analytical task.<h3>HPLC Detection Technologies</h3>HPLC detectors are typically divided into two major categories:<ol><li>Specific detectors (solute-property detectors)</li><li>Bulk property detectors</li></ol>Specific detectors respond to a particular chemical or physical property of the analyte. Ideally, their signal is largely unaffected by changes in mobile phase composition. Common examples include UV/Vis detectors, fluorescence detectors, and mass spectrometers.Bulk property detectors, on the other hand, measure differences between the pure mobile phase and the mobile phase containing analytes. These detectors are considered more universal because they respond to properties shared by many compounds. Refractive index detectors are among the best-known examples, alongside conductivity and light-scattering detectors.Both detector categories have important advantages depending on the application, sample type, and analytical goals.<h3>Common Detector Types Used in HPLC</h3>A wide variety of detectors are available for HPLC systems, ranging from highly universal devices to extremely selective and sensitive technologies.Common detector options include:<ul><li>UV/Vis detectors</li><li>Diode array detectors (DAD/PDA)</li><li>Fluorescence detectors</li><li>Refractive index detectors (RID)</li><li>Conductivity detectors</li><li>Evaporative light scattering detectors (ELSD)</li><li>Charged aerosol detectors (CAD)</li><li>Mass spectrometry detectors (MS)</li></ul>Each technology offers a different balance of sensitivity, selectivity, robustness, operational complexity, and cost.<img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/KNAUER_Detector_overview_with_characteristics_Graphic_by_KNAUER_84b0dd30b1.jpg" alt="KNAUER: Detector overview with characteristics. Graphic by KNAUER." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_KNAUER_Detector_overview_with_characteristics_Graphic_by_KNAUER_84b0dd30b1.jpg 245w," sizes="100vw" width="1293" height="596"><h3>Selecting the Right HPLC Detector</h3>There is no universally ideal detector for all HPLC applications. The best choice depends on several factors, including analyte chemistry, matrix complexity, required detection limits, and instrument compatibility.Careful detector selection is essential for obtaining reliable and meaningful analytical results.The following considerations can help guide the selection process.<h4>1. Chemical and Physical Properties of the Analytes</h4>The chemical nature of the compounds being analyzed is one of the most important selection criteria.Questions to consider include:<ul><li>Do the analytes contain chromophores?</li><li>Are the compounds naturally fluorescent?</li><li>What is their polarity?</li><li>What is their molecular weight?</li><li>Are they volatile or non-volatile?</li></ul>Compounds containing UV-active groups are often well suited for UV/Vis detection, while fluorescent molecules can benefit from the high sensitivity of fluorescence detectors. Non-UV-active compounds may require universal detection approaches such as CAD, ELSD, or mass spectrometry.<h4>2. Required Sensitivity</h4>Detector sensitivity determines the minimum concentration that can be reliably detected and quantified.Routine quality control analyses often rely on UV/Vis detectors because they provide sufficient sensitivity for many applications while remaining relatively simple and cost-effective. However, trace-level analysis or highly complex matrices may require more advanced technologies such as:<ul><li>Fluorescence detection</li><li>Charged aerosol detection</li><li>Mass spectrometry</li></ul>These techniques offer significantly lower detection limits and greater analytical specificity.<h4>3. Detector Linearity</h4>Linearity describes how well detector response correlates with analyte concentration across a defined range.A detector with strong linearity ensures accurate quantification and simplifies calibration procedures. Poor linearity can introduce quantification errors, especially when analyzing samples across broad concentration ranges.<h4>4. Dynamic Range</h4>Dynamic range refers to the concentration interval over which the detector can reliably measure analytes.Applications involving samples with highly variable concentrations require detectors capable of maintaining accurate response over a broad range. A wide dynamic range reduces the need for repeated dilutions or multiple analytical runs.<h4>5. Selectivity</h4>Selectivity defines the detector’s ability to distinguish target analytes from other matrix components.This factor becomes particularly important for complex samples containing many interfering substances. Highly selective detectors reduce the risk of overlapping signals and false positives.Mass spectrometry is often advantageous when dealing with unknown compounds or highly complicated sample matrices because it provides structural information in addition to quantitative data.<h4>6. Budget Considerations</h4>Detector cost is another practical factor that laboratories must evaluate.UV/Vis and refractive index detectors are generally among the more economical choices, making them attractive for routine analyses and standard applications.More sophisticated technologies such as fluorescence detection, CAD, or mass spectrometry typically require:<ul><li>Higher initial investment</li><li>Additional maintenance</li><li>More complex infrastructure</li><li>Greater operational expertise</li></ul>However, the overall value of the detector should always be considered in relation to analytical performance requirements and long-term application needs.<h4>7. Instrument and Mobile Phase Compatibility</h4>The selected detector must be compatible with the operational conditions of the HPLC system.Important parameters include:<ul><li>Flow rate range</li><li>Pressure limitations</li><li>Mobile phase composition</li><li>Solvent compatibility</li></ul>Some detectors are highly sensitive to mobile phase composition or gradient changes, while others tolerate a broader range of operating conditions.Ensuring proper compatibility helps prevent baseline instability, signal interference, and reduced detector performance.<h4>8. Destructive vs. Non-Destructive Detection</h4>Another important consideration is whether the analytical workflow involves only detection or also preparative purification.Non-destructive detectors allow collected fractions to remain intact after detection, which is highly beneficial for preparative chromatography and purification workflows.Destructive detectors, by contrast, consume or alter the sample during measurement and are therefore more suitable for analytical applications where sample recovery is not required.<h4>9. Reliability and Ease of Operation</h4>Practical usability is often just as important as analytical performance.Some detectors are designed for straightforward plug-and-play operation, while others require:<ul><li>Frequent calibration</li><li>Specialized maintenance</li><li>Dedicated gas supplies</li><li>Vacuum systems</li><li>Advanced operator expertise</li></ul>Laboratories should evaluate the available technical support, user experience, and maintenance capabilities before implementing more sophisticated detection technologies.Reliable operation and manageable maintenance requirements can significantly improve laboratory productivity and reduce downtime.Choosing the correct HPLC detector starts with understanding both the sample and the analytical objective. Rather than selecting a detector simply because it is available, laboratories should ensure that the technology aligns with the chemistry of the analytes, required performance characteristics, and workflow demands.In many cases, combining multiple detectors within a single HPLC system can provide even greater analytical insight by delivering complementary information from the same chromatographic separation.<img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/KNAUER_Flow_scheme_for_detector_selection_Graphic_by_KNAUER_486004045f.jpg" alt="KNAUER: Flow scheme for detector selection. Graphic by KNAUER." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_KNAUER_Flow_scheme_for_detector_selection_Graphic_by_KNAUER_486004045f.jpg 245w," sizes="100vw" width="1316" height="808">Interested in learning more? Future articles will explore individual HPLC detection techniques in greater detail, including their operating principles, application areas, and practical advantages for different analytical workflows.

A comprehensive guide for selecting the appropriate HPLC detector

News from LabRulezLCMS Library - Week 20, 2026

Article

Product

Application

Scientists

Science and research

The University of Chemistry and Technology, Prague is a natural centre of first-rate study and research in the area of chemistry in Czechia and is one of the country's largest educational and research institutions focused on technical chemistry, chemical and biochemical technologies, material and chemical engineering, food chemistry, and environmental studies.

UCT Prague

Everything from the world of analytical chemistry in one place. We connect people in solving their problems. At Labrulez you will find all the necessary information easily, quickly and clearly. Stop searching and start finding.

LabRulez

Based in Berlin, KNAUER is a medium-sized, owner-managed company that has been serving the sciences since 1962. We develop and manufacture scientific instruments of superior quality for liquid chromatography, including: Analytical HPLC/UHPLC, Preparative HPLC, Fast protein liquid chromatography (FPLC), Multi-column chromatography/Simulated moving bed (SMB) chromatography. High pressure dosing, in-line detection systems, and osmometry are additional business areas.

KNAUER

Separation Science

Metrohm

Honeywell

VWR/Avantor

C&EN

Water content can affect product quality, texture, shelf-life, chemical stability and reactivity across a wide variety of industries.
Karl Fischer titration is a universally accepted method for measuring water content in all types of substances, including chemicals, oils, pharmaceuticals and food.
In this webinar we will share best practices in KF titration so that users can be confident in their moisture-content results.
<blockquote>
Presenter: Allison Reiter
</blockquote>
<blockquote>
Allison Reiter is the Global Product Manager for Honeywell’s Hydranal Karl Fischer titration reagents. She has a BS in Chemical Engineering and has held various technical roles across Honeywell since joining the company in 2014
</blockquote>

Avantor: Get the most out of your Karl Fischer titration

Get the most out of your Karl Fischer titration

In this webinar we will share best practices in KF titration so that users can be confident in their moisture-content results.

Water impacts battery materials at various levels, ranging from the molecular structure to overall performance. It can react with salts, causing corrosion and short circuiting and, ultimately, a reduction in battery life.
Karl Fischer titration, known for its accuracy in measuring trace amounts of water, is an ideal method for monitoring water content in various battery components. This includes not only cathode and anode raw materials, but also electrolytes and separators used in the battery manufacturing process. 
During this webinar, Dr. Roman Neufeld, Sr. Research Scientist from Honeywell, will discuss recommended coulometric KF reagents for battery electrolytes with and without reactive additives. Eduardo Simões, Product Specialist at Metrohm USA, will discuss how to analyze battery materials using Karl Fischer titration and provide an overview of other titration battery applications. 
Key Learning Objectives:
<ul>
<li>Learn how to analyze battery liquid samples for moisture and avoid side reactions with special KF reagents.
</li>
<li>Understand how the KF Oven technique works for solid materials and for liquid samples that cause side reactions with direct inject.
</li>
<li>Learn about the main potentiometric titration applications for battery materials such as lithium salts, HF, and pure metals like Ni/Co/Mn.
</li>
</ul>
Who Should Attend:
<ul>
<li>QC analysts performing KF titration on battery raw materials, electrolytes, and separators. 
</li>
<li>R&amp;D scientists searching for improvements on their research projects. 
</li>
<li>Anyone from the battery market wanting to learn about how important this analysis is for their materials and products.
</li>
</ul>
<blockquote>
Presenter: Dr. Roman Neufeld (Sr. Research Scientist, Honeywell)
</blockquote>
<blockquote>
Presenter: Eduardo Simões (Product Specialist, Metrohm USA)
</blockquote>
<blockquote>
Presenter: Ann Thayer (Contributing Editor, C&amp;EN Media Group)
</blockquote>

C&EN: Moisture Matters: Accurate Water Determination in Battery Materials

Moisture Matters: Accurate Water Determination in Battery Materials

Recommended coulometric KF reagents for battery electrolytes with and without reactive additives.

Accurate Water Determination in Battery Materials

In this webinar, our expert speakers will focus on the Karl Fischer titration process, quality assurance, and relevant regulations such as pharmacopeias to improve application accuracy.
It will be of particular interest to people developing Karl Fischer applications or qualifying Karl Fischer titrations, especially lab technicians, lab managers, and quality control or assurance managers.
By attending this webinar, you will gain insight into the following:
<ul>
<li>Overview of the Karl Fischer titration process
</li>
<li>Tips to optimize your Karl Fischer application performance
</li>
<li>How to fulfill Quality Assurance Aspects and get an insight into regulations such as United States Pharmacopeia (USP) and European Pharmacopoeia (Ph. Eur.)
</li>
<li>Understanding of the Official Medicines Control Laboratory (OMCL) guidelines concerning the Karl Fischer oven system
</li>
</ul>
<blockquote>
Presenter: Bettina Straub-Jubb (Global Product Manager, Karl Fischer Titration at the life science business of Merck KGaA, Darmstadt, Germany)
</blockquote>
<blockquote>
Bettina is the Global Franchise Product Manager and lead expert for titration at Merck Life Science KGaA based in Darmstadt, Germany. She has more than 20 years’ experience in the instrumental analysis business, especially with Karl Fischer titration, general titration, reference materials, and diagnostics. Her responsibilities include improving and simplifying titration processes, driving new technologies, and advancing applications and reagents. She always works closely with the QC and application laboratories for titration as well as with the R&amp;D department to develop titration continuously. Bettina is a graduate engineer and holds a degree in Chemical Technology from the University of Applied Sciences, Darmstadt, Germany, and a degree as a Medical Technical Laboratory Assistant from the Medical Technical Academy Esslingen, Germany.
</blockquote>
<blockquote>
Presenter: Thomas Hitz (Application Chemist, Mettler Toledo)
</blockquote>
<blockquote>
Thomas Hitz studied biochemistry at the University of Zurich and completed his PhD in 2003 at the Swiss Federal Institute of Technology Zurich in the Department of Material Sciences and Post Doctorate in 2006. He provides market support at Mettler Toledo and has more than 15 years&#39; experience in application development, sales, and customer training, and as a technical advisor for product development. He provides support for complex automation solutions and is an expert in automated Karl Fischer titration.
</blockquote>

Separation Science: How to Fulfil Regulatory Compliance with Your Karl Fischer Titration Process

How to Fulfil Regulatory Compliance with Your Karl Fischer Titration Process

Karl Fischer titration process, quality assurance, and relevant regulations such as pharmacopeias to improve application accuracy.

Regulatory Compliance & Your Karl Fischer Titration Process

The pH value is one of the most important and therefore also one of the most frequently measured parameters in analytical chemistry.
Sometimes, a simple pH test strip will do for a quick check; however, a precise pH meter and the right choice of the pH electrode are a must when reliable results are needed. 
In our educational webinar on November 14, Doris Hoffmann, Product Manager at the Competence Center Titration at Metrohm International Headquarters, will show you that the determination of the pH value can be quite simple, if you pay attention to a few critical details. 
Whether you perform a pH measurement for the first time or are a routine user, we are sure there is room for improvement. Join this webinar and make the next step towards higher accuracy and better reproducibility of your pH measurement results.
Register &amp; participate in this webinar to learn:
<ul>
<li>What the pH value is and how it is measured
</li>
<li>The functional components and design of a pH electrode
</li>
<li>Which kind of pH electrode is suitable for which application
</li>
<li>What parameters are determined during a pH calibration, and what values are acceptable
</li>
<li>How to properly care for and maintain a pH electrode
</li>
</ul>
<blockquote>
Presenter: Doris Hoffmann (Product Manager at CC Titration, Metrohm International Headquarters)
</blockquote>
<blockquote>
Doris Hoffmann has studied chemistry and has specialized in analytical chemistry and electrochemistry in her Master`s degree. She joined Metrohm International Headquarters in 2021 as a Product Manager and is responsible for the extensive portfolio of Metrosensors including pH electrodes, electrodes for titration, and more.
</blockquote>

Metrohm: The basics of precise pH measurement

The basics of precise pH measurement

The determination of the pH value can be quite simple, if you pay attention to a few critical details.

Are complex and/or contaminated samples making your titrations a challenge? There is no suitable potentiometric sensor available for your analyte? We recommend you consider thermometric titration (TET). Instead of a potentiometric sensor, thermometric titration uses a maintenance-free temperature sensor and records the temperature change in the sample solution during the titration reaction: The endpoint is registered when the titration reaction has stopped and with it any change in the temperature of the sample solution. 
Thermometric titration provides results in less than 3 minutes and hardly any sample preparation is required. The thermometric sensor can be stored dry, and you can use this sensor for different applications such as acid number (ASTM D8045) and base number in petrochemical samples, NPK in mineral fertilizers, sodium in foodstuffs, and many more. 
Register now for this webinar and learn more about the many benefits of thermometric titration for your routine analytics! 
Key learnings of this webinar:
<ul>
<li>Understanding the principles of thermometric titration
</li>
<li>Getting an idea of the many applications of thermometric titration in various industries (food, petrochemicals, fertilizers, ...)
</li>
<li>Learning how to implement thermometric titration in your laboratory
</li>
<li>Learning how thermometric titration can be automated using a sample robot
</li>
</ul>
<blockquote>
Presenter: Dr. Mona Wagner (Application Specialist, Metrohm International Headquarters)
</blockquote>
<blockquote>
Mona Wagner studied chemistry at the University of Marburg (Germany) and earned her PhD from the Federal Technical University (ETH) Zurich (Switzerland). She joined Metrohm in 2020 as an application specialist and has gained extensive practical experience in various titration techniques, including thermometric titration.
</blockquote>

Metrohm - Thermometric titration: What it is and how it makes your most challenging samples a walk in the park

Thermometric titration: What it is and how it makes your most challenging samples a walk in the park

Thermometric titration can help with challenging samples

<h4>Overview:</h4>
Water impacts battery materials at various levels, ranging from the molecular structure to overall performance. It can react with salts, causing corrosion and short circuiting and, ultimately, a reduction in battery life. Karl Fischer titration, known for its accuracy in measuring trace amounts of water, is an ideal method for monitoring water content in various battery components. This includes not only cathode and anode raw materials, but also electrolytes and separators used in the battery manufacturing process.
During this webinar, Dr. Roman Neufeld, Sr. Research Scientist from Honeywell, will discuss recommended coulometric KF reagents for battery electrolytes with and without reactive additives. Michael Margreth, Sr. Product Specialist Titration at Metrohm International Headquarters, will discuss how to analyze solid battery materials using Karl Fischer oven methodology.
Key Learning Objectives:
<ul>
<li>Learn how to analyze battery liquid samples for moisture and avoid side reactions with special KF reagents.
</li>
<li>Understand how the KF Oven technique works for solid materials.
</li>
</ul>
Who Should Attend:
<ul>
<li>QC analysts performing KF titration on battery raw materials, electrolytes, and separators.
</li>
<li>R&amp;D scientists searching for improvements on their research projects.
</li>
<li>Anyone from the battery market wanting to learn about how important this analysis is for their materials and products.
</li>
</ul>
<blockquote>
Presenter: Dr. Roman Neufeld (Senior Research Scientist, Honeywell)
Roman works as a Senior Research Scientist, leading the development of new and innovative Karl Fischer (KF) reagents. As one of the major experts in the KF water determination methodology, he also provides superior support to a global customer base on technical queries and application development for KF titration. His newest patent-pending development includes the first commercially available alcohol-and imidazole-free KF reagents for highly accurate water determination in modern lithium-ion battery electrolytes.
</blockquote>
<blockquote>
Presenter: Michael Margreth (Senior Product Specialist, Metrohm Schweiz AG)
Michael Margreth is a Senior Product Specialist for Karl Fischer titration at Metrohm International Headquarters in Herisau, Switzerland. Previously, he worked as Application Specialist for Titration at Metrohm AG. He studied chemistry at the University of Basel, Switzerland and at the FHBB in Muttenz, Switzerland. He obtained his degree in chemistry and joined Metrohm in 2005.
</blockquote>

Metrohm: MOISTURE MATTERS: ACCURATE WATER DETERMINATION IN BATTERY MATERIALS

MOISTURE MATTERS: ACCURATE WATER DETERMINATION IN BATTERY MATERIALS

ACCURATE WATER DETERMINATION IN BATTERY MATERIALS

Get ready to dive into the thrilling world of process analytics - game show style!
Join us for an engaging and educational event as you showcase your knowledge by answering questions to discover how process analyzers can be equipped with titration, ion chromatography or spectroscopy to solve even the toughest challenges.
Our hosts, Elena Hagemann, Product Manager for Process Spectroscopy, and Kraig Kmiotek, Product Manager for Process Wet Chemistry, will test your knowledge while introducing the FUNdamentals of process analytics and unraveling practical insights that can be applied in real-world situations.
Whether you&#39;re a seasoned expert or just curious about the basics, this interactive event promises to educate and entertain, combining the fun of a game show with the science and engineering of process analytics.
Register now, don’t miss out on the end-of-the-year fun!
<blockquote>
Presenter: Kraig Kmiotek (Product Manager for Process Wet Chemistry, Metrohm USA)
</blockquote>
<blockquote>
Kraig Kmiotek is the Product Manager for Process Wet Chemistry at Metrohm USA and has a Bachelor of Science in Chemistry from Purdue University. Since joining the Process Analytics team, his focus has been on application work, analyzer design and programming and customer and internal training of process analyzers. He is currently responsible for understanding customer requirements, driving product and service development, and strategic product marketing and demand generation.
</blockquote>
<blockquote>
Presenter: Elena Hagemann (Product Manager for Process Spectroscopy, Metrohm USA)
</blockquote>
<blockquote>
Elena Hagemann is the Product Manager for Process Spectroscopy at Metrohm USA in Riverview, Florida. She holds a master’s degree in Analytical and Bioanalytical Chemistry from the Applied University of Aalen, Germany, where her master thesis focused on Karl Fischer Titration, NIR Spectroscopy, and Multivariate Data Analysis. Elena joined the Metrohm Group in August 2013 and started with Metrohm USA in January 2018, focusing on Raman and NIR Spectroscopy.
</blockquote>

Metrohm: Process Analytics Showdown: Putting the Fun in Fundamentals

Process Analytics Showdown: Putting the Fun in Fundamentals

Karl Fischer (KF) titration is the primary technique for moisture analysis in many industries.
Although the technique is well-defined, sample preparation and injection are greatly impacted by human error. Even with robust SOPs, slight differences in sample handling by analysts can deliver troubling inconsistencies in results.
Attend this webinar to hear Doug Clark share tips on how you can reduce the impact of human error and improve the accuracy and repeatability of your Karl Fischer results. Doug is an expert with over 30 years of experience in Karl Fischer titration. Our webinar session will conclude with a live question and answer forum moderated by Kerri-Ann Blake, Product Specialist for Titration at Metrohm. This session will answer your questions and present the latest technologies to improve the accuracy of your KF results. 
Key Learning Objectives
<ul>
<li>Learn sample handling techniques to improve KF titration results
</li>
<li>Receive tips for improving KF titration accuracy with proper reagent selection
</li>
<li>Understand how 3rd party devices can be added to titration systems to improve workflow and remove human error
</li>
</ul>
Who Should Attend
<ul>
<li>Analysts performing KF titration who are interested in identifying how human error might be skewing their results
</li>
<li>Lab managers looking to increase accuracy by learning simple tips they can share with their lab staff
</li>
<li>Anyone interested in learning how auxiliary devices, such as an oven, can be integrated into KF titration systems to increase accuracy and repeatability
</li>
</ul>
<blockquote>
Presenter: Doug Clark (Karl Fischer Expert)
</blockquote>
<blockquote>
Doug Clark has over 30 years of experience dedicated to Karl Fischer titration. His extensive career includes developing KF reagents and applications as well as having lead the Honeywell and Sigma Aldrich HYDRANAL Technical Center in St. Louis.
</blockquote>
<blockquote>
Presenter: Dr. Kerri-Ann Blake (Titration Product Manager, Metrohm USA)
</blockquote>
<blockquote>
Dr. Kerri-Ann Blake is the Titration Product Manager at Metrohm USA in Riverview, Florida. She earned her PhD in Materials Science and Engineering at the University of Florida where she was a researcher at the Particle Engineering Research Center. In her years with Metrohm USA, she has performed proof of concept studies, internal and external trainings and provided applications support. Kerri-Ann plays a key role in product support and product marketing and also has experience developing and implementing ASTM methods.
</blockquote>

Metrohm: Impact of Human Error in Karl Fischer Titration

Impact of Human Error in Karl Fischer Titration

How you can reduce the impact of human error and improve the accuracy and repeatability of your Karl Fischer results.

Along with gravimetry, titration is one of the oldest and most reliable quantitative analytical methods known. It is generally used as the primary reference method for other, mostly qualitative, analytical methods and it is indispensable for routine analysis in the laboratory.
In this webinar, Robert Tobler, Senior Application Specialist Titration at Metrohm International Headquarters, will give you an introduction to the basics of titration. After a brief excursion into the theory, Robert will demonstrate how to perform a titration step by step on a modern, automatic titrator with a photometric or an electrochemical sensor.
During a Q&amp;A session at the end of the webinar you can ask Robert any question you may have about titration. Join us on March 27; we are looking forward to welcoming you in this webinar!
<h5>Key learnings of this webinar:</h5>
<ul>
<li>The most important measuring modes for potentiometric titration and application examples from various industries</li>
<li>How to optimize your titration results for higher accuracy and better reproducibility</li>
<li>Common mistakes and how you can avoid them</li>
<li>How to properly handle and take care of your electrodes</li>
</ul>
<h5>Who should attend?</h5>
Lab technicians, chemists, and everybody else using titration for their routine analytics.
<blockquote>
Presenter: Robert Tobler (Senior Application Specialist Titration, Metrohm International Headquarters)
</blockquote>
<blockquote>
After his apprenticeship as a chemical laboratory technician and further training as a biomedical analyst, Robert Tobler studied chemistry at the Zurich University of Applied Science in Waedenswil, Switzerland. He joined Metrohm in 2015 as an application specialist titration. Since then, Robert has become an expert in applied titration sharing his expertise through the global network of Metrohm subsidiaries.
</blockquote>

Metrohm - The Basics of Titration Demonstrated and Explained

The Basics of Titration Demonstrated and Explained

Introduction to the basics of titration and performing a titration step by step on a modern, automatic titrator with a photometric or an electrochemical sensor.

A surge in the number of samples can push everyone in the laboratory to their limits. The risk of human error is considerable, especially when complex sample preparation procedures are required and when these procedures are performed manually by different people, such as when staff work in shifts.
In this webinar, Dr. Julian Konsek, Jr. Product Specialist Titration at Metrohm International Headquarters, will show you how to overcome these challenges by automating your routine analysis.
Focusing on titration applications, Julian will present the capabilities of state-of-the- art automation and explain:
<ul>
<li>How automation can systematically reduce the risk of human error</li>
<li>How you can maximise sample throughput</li>
<li>How automation can improve accuracy and reproducibility of your results</li>
<li>How automation can help you better comply with laboratory safety regulations</li>
</ul>
Who should attend?
Lab managers, lab technicians, chemists, and everybody else involved in routine laboratory analysis.
<blockquote>
Presenter: Dr. Julian Konsek (Jr. Product Specialist Titration, Metrohm International Headquarters)
</blockquote>
<blockquote>
Dr. Julian Konsek earned his PhD in 2022 at the University of Konstanz in Physical Chemistry. He joined Metrohm in 2023 as a product specialist for titration automation. Since then, he has gained an extensive experience in automation for all kind of titration systems working for the Competence Center Titration at Metrohm International Headquarters.
</blockquote>

Metrohm: The benefits of automating your titration applications

The benefits of automating your titration applications

In this webinar, we will show you how to overcome challenges associated with titration analysis by state-of-the- art automation.

LCMS webinars focusing on Titration - page 3