<div><p><strong>Mulching films used in agriculture gradually break down and can become a significant source of microplastics in soils. A new study shows that these particles affect not only soil structure but also microbial life and nitrogen cycling.</strong></p><p><i>“We wanted to understand how microplastics from both conventional and biodegradable mulching films influence soil properties, microbial diversity and functions in real conditions,”</i> explains <strong>Klára Šmídová</strong>, the study’s co-author . <i>“That’s why we added realistic amounts of microplastics to soils in experimental plots in Finland, Germany, and Spain, monitored them over two growing seasons, and compared them with the control plots.”</i></p><p>The results revealed that microplastics alter soil characteristics and microbial communities, although not always in the same way. <i>“ However, a consistent finding was the decline in microbial activity associated with key processes, including nitrogen cycling, with negative effects increasing at higher microplastic concentrations and becoming more pronounced in the second year,” </i>Šmídová adds. Because the microplastic levels used in the study reflect amounts already found in European soils, the findings highlight real risks to soil health. <i>“We also found that the effects were stronger in southern than in northern Europe, likely due to differences in climate and soil conditions. This means that protecting soils from plastic pollution will require region-specific risk assessments,”</i> she says.</p><p>Over 60% of European soils are estimated to be unhealthy, with ongoing degradation driven by unsustainable land use, contamination, climate change, and extreme weather events. In response, the EU Parliament adopted the Soil Monitoring Law in October 2025, aiming to achieve healthy soils across Europe by 2050. <i>“The law highlights the role of soil health in food security, water quality, and climate objectives, and establishes consistent soil monitoring across Member States. It includes measures to support farmers and foresters, while also creating a public list of potentially contaminated sites and an indicative watch list of emerging soil contaminants,” </i>explains Klára Šmídová.</p><p>The project brought together 19 research groups across Europe. <i>“After each season, hundreds of samples were colle cted and sent to partners for analysis. This paper focuses on soil properties, microbial diversity, and greenhouse gas emissions. Two more manuscripts—on plastic additives and microplastic fate, and on soil invertebrates—are in preparation. Together, they will give us a complete picture of how agricultural microplastics affect soil ecosystems,” </i>concludes Klára Šmídová.</p><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/RECETOX_Vysledky_nove_studie_ukazaly_ze_mikroplasty_meni_vlastnosti_pudy_a_mikrobialni_spolecenstva_i_v_realnych_podminkach_6ee3413a7b.jpg" alt="RECETOX: New Study Reveals How Agricultural Plastics Impact Soil Health In Real Conditions" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_RECETOX_Vysledky_nove_studie_ukazaly_ze_mikroplasty_meni_vlastnosti_pudy_a_mikrobialni_spolecenstva_i_v_realnych_podminkach_6ee3413a7b.jpg 171w," sizes="100vw" width="738" height="674"></p><blockquote><h4><strong>The Original article</strong></h4><h4><a target="_blank" rel="noopener noreferrer" href="https://www.sciencedirect.com/science/article/pii/S0269749125015866?via%3Dihub#sec2"><span><strong>Conventional and biodegradable agricultural microplastics: effects on soil properties and microbial functions across a European pedoclimatic gradient</strong></span></a></h4><p>Šmídová K., Soinne H., Kim S. W., Tirronniemi J., Meffe R., Redondo-Hasselerharm P. E., Braun M., Rillig M. C., Fritze H., Adamczyk B., Nikama J., Kaseva J., Saartama V., Amelung W., Hurley R., Hofman J., Nizzetto L., Selonen S., Velmala S.</p><p>Environ. Pollut. 386 (2025) 127212</p><p><a href="DOI:%2010.1016/j.envpol.2025.127212"><span>DOI:&nbsp;10.1016/j.envpol.2025.127212</span></a></p><h6>licensed under <a target="_blank" rel="noopener noreferrer" href="https://creativecommons.org/licenses/by/4.0/"><strong>CC-BY 4.0</strong></a></h6></blockquote><h4><strong>Abstract</strong></h4><div><p>Agricultural plastics like mulching films may become a major source of microplastic (MP) soil contamination during their degradation and fragmentation. This study investigates the effects of agricultural MPs from conventional (linear low-density polyethylene, PE) and biodegradable (starch-blended polybutylene adipate co-terephthalate, PBAT-BD) mulching films on soil physicochemical properties, aggregation, microbial diversity and functions, litter decomposition, and greenhouse gases emissions (GHG). For this purpose, MPs were mixed into soils at realistic MP concentrations of 0.005&nbsp;% and 0.05&nbsp;% (w/w) in 2022 on experimental plots in three EU countries representing different pedoclimatic conditions (Finland, Germany and Spain), followed by monitoring of the above-mentioned variables in the subsequent growing seasons 2022 and 2023. We found several significant MP-induced effects for soil properties, aggregation, microbial diversity, litter decomposition, and GHG, but the effect endpoints were less pronounced or varied considerably. Contrarily, microbial activity, contributing to soil functions such as nitrogen cycling, was consistently reduced by both conventional and biodegradable MPs. The reductions were more pronounced after the second season and for the higher MP treatment. As the higher MP concentration (i.e., 0.05&nbsp;% w/w) is environmentally relevant in Europe, our findings emphasize the potential effects of environmentally relevant MP concentrations on soil health. Furthermore, the effects increased from north to south, probably modulated by varying pedoclimatic conditions, inducing reflection of a need for regionally tailored risk</p></div></div>

New Study Reveals How Agricultural Plastics Impact Soil Health In Real Conditions

<h3><strong>Charged for Action: The Hidden Secrets of AEX Oligonucleotide Analysis</strong></h3><p><strong>Oligonucleotides—short, well-defined fragments of DNA or RNA—have become indispensable tools in modern life sciences. They underpin cutting-edge applications in molecular diagnostics, gene therapy, and pharmaceutical development. Before oligonucleotides can be confidently used in research or clinical settings, however, their quality must be rigorously assessed. Purity, sequence integrity, length distribution, and chemical modifications all play a decisive role in their performance.</strong></p><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/KNAUER_Oligos_Made_Easy_Part_2_AEX_2_45eee54fa0.jpg" alt="KNAUER: Oligos Made Easy - Part 2: AEX" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_KNAUER_Oligos_Made_Easy_Part_2_AEX_2_45eee54fa0.jpg 245w," sizes="100vw" width="1534" height="711"></p><p>Among the analytical techniques available today, <strong>anion exchange chromatography (AEX)</strong> stands out as a robust and highly informative method for characterizing oligonucleotides. By exploiting their intrinsic negative charge, AEX provides detailed insight into product composition and impurity profiles that are difficult to obtain by other approaches.</p><blockquote><p><a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/article/6808"><strong>Part 1 of the Oligos series: A Practical Guide to Oligonucleotide Production – From Concept to Final Product</strong></a></p></blockquote><h4><strong>How Scientists Read Oligonucleotides Using Charge</strong></h4><p>At the core of AEX lies a simple but powerful principle: oligonucleotides carry a negatively charged phosphate backbone. This charge increases in a predictable way with strand length and, to a lesser extent, sequence composition. Anion exchange chromatography uses this property to separate oligonucleotides based on differences in charge strength and interaction with a positively charged stationary phase.</p><p>As a result, AEX has become one of the most widely used analytical tools for evaluating oligonucleotide quality. It allows scientists to distinguish full-length products from truncated species, degradation products, or closely related variants—often with single-nucleotide resolution.</p><h4><strong>Why Ion Exchange Works: Binding, Retention, and Release</strong></h4><p>Ion exchange chromatography (IEX) relies on electrostatic interactions between analytes and a charged stationary phase. In the case of oligonucleotides, <strong>anion exchange</strong> is used, where negatively charged molecules interact with positively charged functional groups on the resin—most commonly quaternary amines.</p><p>The system consists of two main components:</p><ul><li><strong>Stationary phase:</strong> A positively charged resin that binds anionic analytes.</li><li><strong>Mobile phase:</strong> An aqueous buffer system, typically combined with a salt gradient, that controls both pH and elution strength.</li></ul><p>Retention in AEX is governed primarily by charge. Molecules with weaker overall charge interact less strongly with the stationary phase and elute earlier, while more highly charged oligonucleotides bind more tightly and require stronger elution conditions.</p><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/KNAUER_Oligos_Made_Easy_Part_2_AEX_Figure_1_IEX_overview_including_elution_order_dependency_on_analyte_charge_3419ad3075.jpg" alt="KNAUER: Oligos Made Easy - Part 2 AEX: Figure 1 IEX overview including elution order dependency on analyte charge." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_KNAUER_Oligos_Made_Easy_Part_2_AEX_Figure_1_IEX_overview_including_elution_order_dependency_on_analyte_charge_3419ad3075.jpg 245w," sizes="100vw" width="1583" height="654"></p><h4><strong>The Five-Step Workflow of Ion Exchange Chromatography</strong></h4><p>AEX separations generally follow a well-established sequence of steps:</p><ol><li><strong>Column equilibration</strong><br>The column is conditioned with the starting buffer until the baseline and pH are stable. This ensures that all charged sites on the resin are ready for interaction.</li><li><strong>Sample loading</strong><br>Samples, prepared in the starting buffer, are introduced under conditions that promote binding of the target oligonucleotides while minimizing retention of unwanted impurities.</li><li><strong>Washing</strong><br>The column is washed with the starting buffer to remove uncharged or weakly interacting components. The baseline stabilizes as these species are flushed away.</li><li><strong>Elution</strong><br>Bound oligonucleotides are released using a salt or pH gradient. Increasing ionic strength introduces competing ions that displace the analytes from the resin. Weakly bound species elute first; strongly charged oligonucleotides elute later.</li><li><strong>Regeneration and re-equilibration</strong><br>A high-salt buffer removes any remaining bound material. The column is then returned to starting conditions in preparation for the next analysis.</li></ol><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/KNAUER_Oligos_Made_Easy_Part_2_AEX_Figure_2_Five_steps_of_IEX_procedure_in_blue_outlined_using_an_example_chromatogram_in_red_38dd70b1b1.jpg" alt="KNAUER: Oligos Made Easy - Part 2 AEX: Figure 2  Five steps of IEX procedure (in blue), outlined using an example chromatogram (in red)." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_KNAUER_Oligos_Made_Easy_Part_2_AEX_Figure_2_Five_steps_of_IEX_procedure_in_blue_outlined_using_an_example_chromatogram_in_red_38dd70b1b1.jpg 245w," sizes="100vw" width="1583" height="674"></p><h4><strong>Why AEX Is Especially Effective for Oligonucleotides</strong></h4><p>The repeating phosphate backbone of oligonucleotides gives rise to a strong and uniform anionic character across a wide pH range (typically pH 7–9). This makes oligonucleotides exceptionally well suited for anion exchange separations.</p><p>Key factors influencing AEX behavior include:</p><ul><li><strong>Charge density:</strong> Longer oligonucleotides carry more negative charges and therefore exhibit stronger retention.</li><li><strong>Resin chemistry:</strong> Strong and weak anion exchangers differ in functional groups and pH stability, influencing selectivity and robustness.</li><li><strong>Buffer composition:</strong> The choice of buffering ions and salt gradients directly affects elution strength and resolution.</li></ul><p>Salt gradients—commonly based on chloride ions—compete with the oligonucleotide backbone for binding sites on the resin. By carefully tuning gradient slope and composition, analysts can achieve highly reproducible and finely resolved separations.</p><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/KNAUER_Oligos_Made_Easy_Part_2_AEX_Figure_3_Ionic_interactions_and_p_H_influence_on_Anion_Exchange_Chromatography_AEX_612e231ba4.jpg" alt="KNAUER: Oligos Made Easy - Part 2 AEX: Figure 3 Ionic interactions and pH influence on Anion Exchange Chromatography (AEX)." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_KNAUER_Oligos_Made_Easy_Part_2_AEX_Figure_3_Ionic_interactions_and_p_H_influence_on_Anion_Exchange_Chromatography_AEX_612e231ba4.jpg 245w," sizes="100vw" width="1547" height="593"></p><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/KNAUER_Oligos_Made_Easy_Part_2_AEX_Figure_4_AEX_buffer_selection_and_gradient_strategy_chromatogram_with_red_buffer_gradient_curve_53fd1bb11b.jpg" alt="KNAUER: Oligos Made Easy - Part 2 AEX: Figure 4 AEX buffer selection and gradient strategy (chromatogram with red buffer gradient curve)." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_KNAUER_Oligos_Made_Easy_Part_2_AEX_Figure_4_AEX_buffer_selection_and_gradient_strategy_chromatogram_with_red_buffer_gradient_curve_53fd1bb11b.jpg 245w," sizes="100vw" width="1600" height="327"></p><h4><strong>AEX as the Gold Standard for Oligonucleotide Separation</strong></h4><p>Anion exchange chromatography has become the method of choice for both analytical and preparative oligonucleotide workflows. Its advantages include:</p><ul><li><strong>High resolving power:</strong> Closely related impurities, including n–1 or n–2 species, can often be separated and quantified.</li><li><strong>Predictable retention behavior:</strong> In many cases, retention time correlates linearly with oligonucleotide length, simplifying identification.</li><li><strong>Compatibility with modified oligonucleotides:</strong> Even chemically modified sequences retain their anionic nature, allowing AEX to resolve both products and related impurities.</li><li><strong>Excellent control via gradient elution:</strong> Salt gradients provide precise tuning of selectivity and resolution.</li></ul><p>Typically, shorter or degraded oligonucleotides elute at lower ionic strength, while full-length products with higher charge density appear later in the chromatogram.</p><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/KNAUER_Oligos_Made_Easy_Part_2_AEX_Figure_5_AEX_Advantages_and_Challenges_7835521e34.jpg" alt="KNAUER: Oligos Made Easy - Part 2 AEX: Figure 5 AEX Advantages and Challenges." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_KNAUER_Oligos_Made_Easy_Part_2_AEX_Figure_5_AEX_Advantages_and_Challenges_7835521e34.jpg 245w," sizes="100vw" width="1535" height="707"></p><h4><strong>Final Perspective: Mastering AEX with Confidence</strong></h4><p>Anion exchange chromatography may appear complex at first, but with thoughtful optimization of pH, buffer composition, and gradient conditions, it becomes a highly precise and reliable tool for oligonucleotide analysis. When properly applied, AEX delivers sharp peaks, excellent resolution, and robust performance—even for challenging sequences and modifications.</p><p>For laboratories developing or refining oligonucleotide workflows, AEX offers both analytical depth and operational confidence.</p><blockquote><p>If you would like to discuss AEX method development or optimization for oligonucleotides, feel free to contact us at <strong>sales@knauer.net</strong>. More insights into practical oligonucleotide analysis will follow in our <i>“Oligos Made Easy”</i> series.</p><p>For technical questions or in-depth discussion, the author can be reached at <strong>kindler@knauer.net</strong></p></blockquote>

Oligos Made Easy - Part 2: AEX

News from LabRulezLCMS Library - Week 07, 2026

Article

Application

Plastics

Environment

Science and research

Poster

RECETOX (Research Centre for Toxic Compounds in the Environment) is an independent department of the Faculty of Science of Masaryk University in Brno, which has been established in 1983. RECETOX is focused on the research and education in cross-cutting area of Environment and Health. It addresses interactions among chemicals, environment, and biological systems, including consequences at local, regional, and global levels.

RECETOX - Centrum pro výzkum toxických látek v prostředí

Everything from the world of analytical chemistry in one place. We connect people in solving their problems. At Labrulez you will find all the necessary information easily, quickly and clearly. Stop searching and start finding.

LabRulez

Based in Berlin, KNAUER is a medium-sized, owner-managed company that has been serving the sciences since 1962. We develop and manufacture scientific instruments of superior quality for liquid chromatography, including: Analytical HPLC/UHPLC, Preparative HPLC, Fast protein liquid chromatography (FPLC), Multi-column chromatography/Simulated moving bed (SMB) chromatography. High pressure dosing, in-line detection systems, and osmometry are additional business areas.

KNAUER

Tosoh Bioscience

Axcend

The Analytical Scientist

Phenomenex

Thermo Fisher Scientific

Shimadzu Corporation

Waters Corporation

<p><strong>Interactions of analytes with metal surfaces in high-performance liquid chromatography (HPLC) instruments and columns have been reported to cause deleterious effects ranging from peak tailing to a complete loss of the analyte signal. These effects are due to the adsorption of certain analytes on the metal oxide layer on the surface of the metal components.</strong></p>
<p>We have developed a novel surface modification technology and applied it to the metal components in ultra HPLC (UHPLC) instruments and columns to mitigate these interactions. A hybrid organic−inorganic surface, based on an ethylene-bridged siloxane chemistry, was developed for use with reversed-phase and hydrophilic interaction chromatography. We have characterized the performance of UHPLC instruments and columns that incorporate this surface technology and compared the results with those obtained using their conventional counterparts. </p>
<p>We demonstrate improved performance when using the hybrid surface technology for separations of various small molecules as well as nucleotides, phosphopeptides, and oligonucleotides. The hybrid surface technology was found to result in higher and more consistent analyte peak areas and improved peak shape, particularly when using low analyte mass loads and acidic mobile phases. Reduced abundances of iron adducts in the mass spectrum of a peptide were also observed when using UHPLC systems and columns that incorporate hybrid surface technology. These results suggest that this technology will be particularly beneficial in UHPLC/mass spectrometry investigations of metal-sensitive analytes.</p>
<p>Join this session to:</p>
<ul>
<li><strong>Learn about non-specific adsorption and the challenges it can cause</strong></li>
<li><strong>Learn about current workarounds that scientists currently use to mitigate non-specific adsorption</strong></li>
<li><strong>Learn about MaxPeak HPS technology and how this prevent non-specific adsorption across many different workflows</strong></li>
</ul>
<blockquote>
<p><strong>Presenter:</strong> Kim Haynes (Director of Chemistry Marketing and Product Management , Waters Corporation)</p>
</blockquote>
<blockquote>
<p>Kim Haynes is a Director of Chemistry Marketing and Product Management in the Consumables and Lab Automation Group at Waters Corporation.<br/>Kim manages MaxPeak High Performance Surface Technologies as well as a variety of other programs. Prior to this position, she has worked with Waters Corporation as a chemistry sales specialist and a business development manager.</p>
</blockquote>
<blockquote>
<p>During her 23 years at Waters, Kim has focused on helping scientists and colleagues understand chromatographic separations and sample preparation. Before joining Waters Corporation, Kim worked as an analytical chemist at Bacardi Martini. She holds a BS in Chemistry from Ohio University.</p>
</blockquote>


The Analytical Scientist: Increasing method reproducibility by eliminating unwanted analyze surfaced interactions

Increasing method reproducibility by eliminating unwanted analyze surfaced interactions

Performance when using the hybrid surface technology for separations of various small molecules as well as nucleotides, phosphopeptides, and oligonucleotides. 

Reproducibility & eliminating unwanted surfaced interactions

<p><strong>WEOF is back for 2023, and this time we will do it 100% virtually to allow even more people to participate. But don&#39;t expect to see only some presentations! We have put together for you an interesting agenda with Live Demos, customer talks, and the chance to ask our experts your questions. It will be a great opportunity to learn more about the advancements in oligonucleotide analysis and about the analytical challenges and successes of your peers.</strong></p>
<ul>
<li>Join our virtual Live Demos</li>
<li>Hear from other scientists like you about their approaches and experiences</li>
<li>Stay up to date with the latest analytical developments for nucleic acids</li>
</ul>
<p>The agenda will be a mix of talks from the scientific community and Waters presentations. We will discuss current analytical needs and show some Live Demos of LC/MS solutions and software:</p>
<h3><strong>AGENDA</strong></h3>
<h5><strong>10:00 Welcome and Short Introduction</strong></h5>
<ul>
<li><strong>Speaker:</strong> David Muñoz (Waters Corporation)</li>
</ul>
<h5><strong>10:05 Waters Live Demo: Impurity Analysis by LC/MS</strong></h5>
<ul>
<li><strong>Speaker:</strong> Emma L Harry (Waters Corporation)</li>
</ul>
<h5><strong>10:25 Customer Talk: Cytiva Sweden AB</strong></h5>
<h5><strong>Analysis of Oligonucleotides using BioAccord LC-MS</strong></h5>
<ul>
<li><strong>Speaker:</strong> Filip Bergqvist (Senior Scientist)</li>
</ul>
<p>Topic: intact analysis of oligonucleotides, specifically development/performance with IP-RPC</p>
<h5><strong>10:40 Customer Talk: BioNTech Manufacturing GmbH</strong></h5>
<h5><strong>QC Analytics for mRNA therapies</strong></h5>
<ul>
<li><strong>Speaker:</strong> Jonas Sander</li>
</ul>
<p>Topic: How BioNTech has masterdto set up QC analytics for mRNQ therapies and the added value of Waters solutions</p>
<h5><strong>10:55 MaxPeak Premier Technology for Oligonucleotide Analysis</strong></h5>
<ul>
<li><strong>Speaker:</strong> Ali Tiss (Waters Corporation)</li>
</ul>
<h5><strong>11:10 Waters Live Demo: Software</strong></h5>
<h5><strong>CONFIRM &amp; INTACT app</strong></h5>
<ul>
<li><strong>Speaker:</strong> Chris Knowles (Waters Corporation)</li>
</ul>
<h5><strong>11:30 Q&amp;A with Waters</strong></h5>
<p>You ask - our application chemists answer</p>
<h5><strong>11:45 Closing</strong></h5>
<h4><strong>INVITED GUEST SPEAKER</strong></h4>
<h5><strong>Filip Bergqvist</strong> (Senior Scientist, Cytiva Sweden AB)</h5>
<p>Filip is leading bioanalytical activities to support product development and application work for bioprocessing. His main expertise is LC-MS, where he is developing methods for characterization of proteins and nucleic acid therapeutics. </p>
<h5><strong>Dr. Jonas Sander</strong> (BioNTech Manufacturing GmbH)</h5>
<p>(further info will follow soon)</p>
<h4><strong>YOUR WATERS HOSTS</strong></h4>
<h5><strong>David Muñoz</strong> (Principal Biopharma Market Development Manager, Waters Corporation)</h5>
<p>David will be your host for this event. He completed his PhD in Biochemistry at Nottingham Trent University, focused on the effects of toxins on glioma cells. After this he joined Lonza to work as a research and development scientist using different analytical techniques to characterize antibodies and proteins. Later he worked for Thermo Fisher Scientific as applications specialist and Biopharma Expert, and as business development manager in two CDMOs,  focusing on antibodies and viral vector projects. David joined Waters in June 2022 as principal biopharma market development manager.   </p>
<h5><strong>Emma L Harry</strong> (European Applications Laboratory Team Manager, Waters Corporation)</h5>
<p>Emma completed her PhD in Analytical Chemistry at Loughborough University, focused on the development of ion mobility mass spectrometry for complex mixtures.</p>
<p>After her post doctoral research at University of Leicester and having worked as analytical scientist at Hall Analytical, using a variety of analytical techniques, Emma joined Waters in June 2019 as a senior applications chemist in the Demo lab in Wilmslow. In 2021 she was promoted to European applications lab manager.</p>
<h5><strong>Chris Knowles</strong> (Senior Applications Scientist, Waters Corporation)</h5>
<p>Chris is currently working as a Principal Domain Specialist (Applications science) at Waters, having been with the company for 5 years.  He works with the Waters informatics teams to help deliver the waters_connect software applications. His focus has been on the CONFIRM Sequence application.  His background is in Bioprocessing, having worked at FUJIFILM Diasynth Biotechnologies for 3 years as a principal scientist in Mammalian cell culture, following completion of a PhD in mammalian cell line engineering at the University of Aberdeen in 2016.</p>
<h5><strong>Ali Tiss</strong> (Sales Specialist Biopharma Chemistry, Europe South, Waters Corporation)</h5>
<p>Ali completed his Ph.D. in biochemistry and structural biology at the Mediterranean University (CNRS, Marseille, France), where he contributed to the R&amp;D of the anti-obesity drug, Xenical®, developed by Roche Ltd. Then, he spent about 20 years of academic research (France, UK, Kuwait) in biochemistry, liquid chromatography, mass-spectrometry, and proteomics, focusing on detecting and characterizing biomarkers related to human diseases (cancer, diabetes, obesity). Ali joined Waters in October 2021 as a BioPharma and New Modalities Specialist in Chemistry for Southern Europe.</p>


Waters Corporation: Waters European Oligonucleotides Forum 2023

Waters European Oligonucleotides Forum 2023

We have put together for you an interesting agenda with Live Demos, customer talks, and the chance to ask our experts your questions. 

<p><strong>Axcend Focus LC Free Webinar</strong></p>


What's New in HPLC?

Axcend Focus LC Free Webinar by Axcend team.

<p><strong>This meeting covers the latest trends and techniques to meet current water and environmental testing demands for emerging contaminants and evolving regulations. Our guest speakers from the water industry and environmental research and testing laboratories will share their expertise on their latest work, analytical workflows, and automation.</strong></p>
<p>Join us and discover valuable insights to maximise testing productivity and instrument uptime, simplify workflows and standardise data workup.</p>
<h3><strong>June 28 (Day 2) – Evolving regulations</strong></h3>
<p>This session will explore disinfection by-products, haloacetic acids, heavy metals, volatiles/semi-volatiles analysis.</p>
<p>Attend the whole day or selected presentations only.</p>
<h5><strong>10:00</strong> Online access opens (Streaming begins 09:30 BST/10:30 CEST)</h5>
<h5><strong>10:30 Opening of the event, welcome and introduction</strong></h5>
<ul>
<li>Presenter: Ed George (Thermo Fisher Scientific)</li>
</ul>
<h5><strong>10:50 Beyond compliance - use of the Orbitrap Exploris 120 HRAM-LC-MS for the monitoring of catchments</strong></h5>
<ul>
<li>Presenter: Mark Collins (Northern Ireland Water)</li>
</ul>
<h5><strong>11:20 Utilizing IC-MS/MS for the analysis of halo acetic acids and disinfection by products in drinking water</strong></h5>
<ul>
<li>Presenter: Chris Pegg (Anglian Water)</li>
</ul>
<h5><strong>11:50 GC-MS analysis of environmental samples using automated sample preparation</strong></h5>
<ul>
<li>Presenter: John Quick (ALS Environmental Ltd.)</li>
</ul>
<h5><strong>12:20</strong> Break</h5>
<h5><strong>12:40 Development of an ion chromatography method with EC and MS detection for the analysis of bromide and the disinfection by products bromate, chlorate and chlorite</strong></h5>
<ul>
<li>Presenter: Joseph Scott (Affinity Water)</li>
</ul>
<h5><strong>13:10 High throughput nutrients analysis - developing methodologies for environmental and wastewater matrices</strong></h5>
<ul>
<li>Presenter: Red Godwin (Thermo Fisher Scientific)</li>
</ul>
<h5><strong>13:40 Performing the analysis of trace metals in environmental samples via ICP and ICP-MS</strong></h5>
<ul>
<li>Presenter: Trudy McMurray (Northern Ireland Environment Agency)</li>
</ul>
<h5><strong>14:15</strong> Closing of the live streaming event</h5>


Thermo Scientific: Water & Environmental Analysis User Meeting - Day 2

Water & Environmental Analysis User Meeting - Day 2

Streaming live from our Demo Centre in Hemel Hempstead | Join the complete event or the presentations of your choice only.

<p><strong>As a continuation of our Chromatography of Bioseparations 101 series, we bring to you 2 NEW SESSIONS in our Chromatography of Bioseparations 102 webinar series!  Join us live for our new parts 6 and 7.  If you haven&#39;t had an opportunity to register for Parts 1-5, feel free to regster and listen to them on-demand now.</strong></p>
<p>These new webinars will focus on the analytical separation of biomolecules such as peptides and amino acids.</p>
<p>Reverse-phase chromatography is the predominant technique used for the analyses and or purification of chemically synthesized peptides or peptides generated by the enzymatic digestion of proteins (i.e., peptide mapping). It is advantageous to have a simplified step-by-step approach to develop a method that can be applied to chemically synthesized peptides or protein digests.</p>
<p>Amino acid analysis is required in several different areas of research and is a fundamental tool in various product analytical activities.  Due to the varied properties of amino acids (i.e., acidic, basic, etc.), the separation and detection represents an analytical challenge and depending on throughput, sample preparation can be laborious.  </p>
<p>Who should attend?</p>
<ul>
<li><strong>Those interested in a refresher on foundational principles and tips of reverse phase separations of peptides.</strong></li>
<li><strong>Those interested in  how to select columns and mobile phases that are optimal for peptide and amino acid workflows.</strong></li>
<li><strong>Those looking for an overview of industry advancements and new offerings.</strong></li>
</ul>
<h3><strong>PART 7 | June 27th, 2023 | 10:30AM – 12:00PM EDT</strong></h3>
<h3><strong>UNDERSTANDING AMINO ACID ANALYSIS FOR PROTEIN HYDROLYSATES AND CELL CULTURE MEDIA</strong></h3>
<ul>
<li><strong>Understand the challenges of sample preparation, separation, and detection of amino acids in different matrices.</strong></li>
<li><strong>Review analytical techniques that addresses the challenges of amino acid analysis.</strong></li>
</ul>
<blockquote>
<p><strong>Presenter:</strong> Paula Phenix (Principal Product Manager – Consumables and Lab Automation, Waters Corporation)</p>
</blockquote>
<blockquote>
<p>Paula is a Principal Marketing Manager at Waters with a focus on Waters products for amino acid and glycan analysis. Prior to her current role in the Chemistry and Lab Automation Group, she was the Product Marketing Manager for Specialty LC and SFC Systems in the Instruments group. Before joining Waters, she held product management, marketing and sales roles for several companies (Life Technologies, Promega, MilliporeSigma) in the molecular biology, immunology, cell culture and single-use bioprocessing areas. Paula graduated from the University of Delaware with a Bachelor’s Degree in Biology and from Johns Hopkins University with a Masters Degree in Business.</p>
</blockquote>


UNDERSTANDING AMINO ACID ANALYSIS FOR PROTEIN HYDROLYSATES AND CELL CULTURE MEDIA

Understand the challenges of sample preparation, separation, and detection of amino acids in different matrices.

UNDERSTANDING AMINO ACID ANALYSIS

<p><strong>Purification is critical to many scientific workflows. Compounds of interest are rarely found to be the right purity in their native state.  Join Waters and Virscidian for this 6-part webinar series to learn the basics of purification and application of purification principles for more advanced workflows.</strong></p>
<h3><strong>PART 3  | Things to Consider When Configuring Purification Systems and Informatics</strong></h3>
<p>Preparative chromatography is a separation technique where one or more peaks are collected for use in another experiment or process.</p>
<p>In this module, we will discuss the various hardware options available, from pumps and injectors through detection techniques to fraction collection. We’ll also touch on some of the software options available and look at different schemes for triggering fraction collection. </p>
<p>Learning Objectives:  </p>
<ul>
<li><strong>Understand various instrument configuration and the impact on purification</strong></li>
<li><strong>Optimize system performance</strong></li>
</ul>
<blockquote>
<p><strong>Presenter:</strong>  Darcy Shave (Senior Product Manager, Waters Corporation)</p>
</blockquote>
<blockquote>
<p>Darcy Shave is currently aprincipalproduct manager in the Integrated Systems group atWaters Corporation (Milford, MA, USA), responsible for the purification and hardwareautomation products. Darcy has been with Waters for over 20 years, starting as anapplications chemist in the purification group. From there, he moved into a variety ofbusiness development roles before becoming a product manager for purification softwareabout 10 years ago and more recently for the entire purification portfolio.​</p>
</blockquote>
<blockquote>
<p>He has a degree in Agricultural Chemistry from the University of Saskatchewan and workedin value added processing of natural products before joining Waters.</p>
</blockquote>


Waters Corporation: Things to Consider When Configuring Purification Systems and Informatics

Things to Consider When Configuring Purification Systems and Informatics

We will discuss the various hardware options available, from pumps and injectors through detection techniques to fraction collection.

When Configuring Purification Systems and Informatics

<p><strong>This webinar introduces an analytical method that utilizes ion-exchange chromatography to separate and analyze oligonucleotides of different lengths. The primary focus is on eliminating impurities that are produced during the synthesis process.</strong></p>
<p>The method incorporates an inert ultra-high-performance liquid chromatography system, liquid chromatography-tandem mass spectrometry, and automated data analysis software. These components work together to facilitate efficient oligonucleotide development and quality control. The results emphasize the challenges associated with metal-based methods and propose a metal-free approach for precise quantification. Additionally, a high-resolution ion-pair reversed-phase LC-MS system is presented for accurate separation and mass analysis of oligonucleotides. The developed method also offers sensitive, precise, and reproducible results, making it an invaluable tool for various scientific fields, including therapeutic oligonucleotide-based drug development and quality control.</p>
<blockquote>
<p><strong>Speaker: Mridul Mandal, Ph.D.</strong></p>
</blockquote>
<blockquote>
<p>Marketing Manager, Shimadzu Scientific Instruments<br/>Mridul Mandal, Ph.D. is currently working as a market manager -pharma/biopharma at Shimadzu Scientific Instruments. Before entering this position, he held numerous roles in academia and pharma CRO for over 12 years. He has published over 30 peer-reviewed publications and book chapters on mass spectrometry, chromatography, and other instrumentation in biomedical science</p>
</blockquote>


Shimadzu: Oligonucleotide Analysis by LC and LC-MS

Oligonucleotide Analysis by LC and LC-MS

This webinar introduces an analytical method that utilizes ion-exchange chromatography to separate and analyze oligonucleotides of different lengths.

<p><strong>On December 1, 2022 a harmonized standard for General Chapter 621 Chromatography was released. This standard incorporates the USP with 2.2.46. Chromatographic Separation Techniques European Pharmacopoeia (EuPh) and 2.01 Liquid Chromatography Japanese Pharmacopeia (JP) texts. Harmonization of these regulatory guidance provides increased method flexibility through the employment of modernized of chromatographic tools without the need for full monograph re-validation.</strong></p>
<p>The extent to which the various parameters of a compendial chromatographic test may be adjusted without fundamentally modifying the pharmacopeial analytical procedures is defined in U.S. Pharmacopeia (USP) General Chapter 621 Chromatography.</p>
<p>In this presentation, we combine the gradient method adjustments described in this chapter to achieve both column and system modernization for the USP monograph separation of antiviral drug abacavir sulfate. </p>
<h5>Key Learning Objectives:</h5>
<ul>
<li><strong>Learn how to modernize the HPLC column and reduce operation costs using the USP 621 allowable adjustments</strong></li>
<li><strong>Learn how to modernize the LC platform and further reduce operation costs using the USP 621 allowable adjustments</strong></li>
<li><strong>Leverage Empower CDS fields to apply the updated chromatography reporting calculations</strong></li>
</ul>
<h5>Who Should Attend:</h5>
<ul>
<li>Scientists and lab managers involved with compendial methods with a drive towards modernization, productivity and compliance.</li>
</ul>
<blockquote>
<p><strong>Presenter:</strong> Catharine Layton (Principal Scientist, Scientific Operations, Waters Corporation)</p>
</blockquote>


Waters Corporation: Modernizing LC Methods as per Harmonized USP Chapter 621

Modernizing LC Methods as per Harmonized USP Chapter 621

We combine the gradient method adjustments described in this chapter to achieve both column and system modernization for the USP monograph.

<p>Key Learning Points:</p>
<ul>
<li><p><strong>Gain a comprehensive understanding of solvent miscibility and recognize its significance</strong></p>
</li>
<li><p><strong>Develop an in-depth comprehension of how to properly solvent switch a column, and the potential consequences associated with incorrect procedures</strong></p>
</li>
<li><p><strong>Familiarize yourself with a standardized protocol for transitioning an LC column from one solvent system to another, ensuring a seamless solvent switch</strong></p>
</li>
</ul>
<p>Who Should Attend:</p>
<p>Any LC users looking to utilize different modes of separation and are required to switch a column that is shipped with an immiscible solvent to the one method requires</p>
<blockquote>
<p><strong>Presenter:</strong> Genevieve Hodson (Technical Support, Phenomenex)</p>
</blockquote>


Phonomenex: How to Solvent Switch Successfully

How to Solvent Switch Successfully

Gain a comprehensive understanding of solvent miscibility and recognize its significance.

<p><strong>Christian Rohrer, Director of Sales &amp; Support – EMEA at Tosoh Bioscience GmbH, will present the next edition of Tosoh Tuesday, a webinar series held by Chromatography Experts.</strong></p>
<p>The talk introduced various chromatographic methods applied in protein purification such as protein A and L affinity chromatography for antibody-derived molecules, chelate affinity for proteins expressed with His-tag, and ion exchange as a versatile first step for protein purification.</p>
<blockquote>
<p><strong>Presenter:</strong> Christian Rohrer (Director of Sales &amp; Support)</p>
</blockquote>


Tosoh Bioscience: Tosoh Tuesday - Unlocking the Secrets of Recombinant Protein Purification: A Beginner's Guide

LCMS webinars focusing on HPLC - page 34