Ask any analytical chemist which hyphenated technique has become indispensable over the past decade, and the answer will almost certainly be the same: tandem chromatography–mass spectrometry. Whether it is <a target="_blank" rel="noopener noreferrer" href="https://www.organomation.com/preparing-samples-for-lc-ms/ms-analysis">LC–MS/MS</a> quantifying a novel biologic’s metabolic fate, or <a target="_blank" rel="noopener noreferrer" href="https://www.organomation.com/preparing-samples-for-gc-ms/ms-analysis">GC–MS/MS</a> chasing per- and polyfluoroalkyl substances (PFAS) down to sub-part-per-trillion levels in municipal water, the combination of chromatographic separation with the specificity of tandem mass detection has become the analytical workhorse of choice across virtually every regulated sector.Below we examine the key forces propelling this expansion, from biopharma pipeline dynamics and PFAS regulatory mandates to AI-driven automation and reshoring of pharmaceutical manufacturing—and explore what each trend means for laboratories managing sample preparation and analytical workflows.<h3>Biopharma: The Engine Room of LC–MS/MS Demand</h3><h4>GLP-1 Agonists and the Metabolomics Gold Rush</h4>The explosive commercial and scientific interest in GLP-1 receptor agonists—<a target="_blank" rel="noopener noreferrer" href="https://www.sepscience.com/advancing-glp-1-peptide-analysis-chromatography-challenges-and-innovations-12163">semaglutide and related peptide therapeutics </a>for diabetes and obesity—has dramatically expanded the analytical demands placed on tandem MS platforms. Discovery programs require precise quantification of peptide drug candidates and endogenous biomarkers across complex biological matrices. These workflows rely on high-sensitivity LC–MS/MS for pharmacokinetic profiling, receptor occupancy studies, and metabolomics characterization. Metabolomics is, in fact, projected to be the fastest-growing application segment in mass spectrometry, driven in part by its growing role in cancer screening, biomarker discovery, and metabolic disease research.I have been spending more time learning about GLP-1s because this is the hot topic that people want to discuss on the Concentrating on <a target="_blank" rel="noopener noreferrer" href="https://open.spotify.com/show/1wZg67rCjoGju4yhqxI3tL?si=n9GLz9zjSCOrVfXPxFPhng&amp;nd=1&amp;dlsi=52868e027aad4d22">Chromatography podcast</a>. With that said, I was astounded looking at CNBC.com during breakfast this morning to see that <a target="_blank" rel="noopener noreferrer" href="https://www.cnbc.com/2026/03/21/glp-1-diets-restaurants-protein-fiber-weight-loss-drugs.html">one in eight Americans are currently taking one of these drugs such as Ozempic</a>.<h4>Antibody–Drug Conjugates and Gene Therapy: Complexity at Every Step</h4>The ascent of antibody–drug conjugates (ADCs) and gene therapies has introduced a new level of analytical complexity. ADCs blend the target specificity of monoclonal antibodies with the cytotoxic potency of small-molecule payloads—and characterizing the linker chemistry, drug-to-antibody ratio (DAR), and conjugate stability demands high-resolution LC–MS/MS at every stage from discovery through QC release testing. Gene therapy vectors require equally rigorous characterization of impurities, aggregates, and empty/full capsid ratios.This translates into a direct need for low-evaporation, high-recovery concentration methods that preserve labile protein structures and maintain sample integrity prior to LC–MS/MS injection. <a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/article/5392">Nitrogen evaporation workflows</a> that minimize oxidative stress and maintain sample temperature are increasingly critical in these pipelines.<h4>Precision Medicine and Multi-Omics Integration</h4>The shift toward precision medicine has accelerated adoption of high-throughput, multi-analyte MS platforms. Proteomics, metabolomics, and lipidomics workflows are converging on a single analytical platform—tandem MS—and generating datasets of a scale and complexity that were unimaginable ten years ago.Make no mistake: the triple quadrupole serves an irreplaceable role in quantitative targeted analysis. At the same time, <a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/products?category=134&amp;manufacturerItems=3">Orbitrap </a>and Q–TOF hybrid platforms are growing rapidly as researchers demand full-scan accurate mass for untargeted discovery.<h3>Environmental Mandates: PFAS Testing Drives Adoption</h3>Environmental testing is something that every person, chemist or not, can understand the importance of. Mounting mandates from the U.S. Environmental Protection Agency and European regulators regarding PFAS—the “forever chemicals” whose extraordinary environmental persistence is matched only by the analytical challenge of detecting them at trace levels—have created immediate, compliance-driven investment in LC–MS/MS infrastructure.<h4>The Regulatory Framework Chemists Need to Know</h4>The EPA has codified a suite of LC–MS/MS-based methods as the analytical standard for PFAS in water and solid matrices:<ul><li><a target="_blank" rel="noopener noreferrer" href="https://blog.organomation.com/blog/epa-533-drying-down-pfas-samples-after-spe">EPA Method 533</a>: Weak anion exchange SPE combined with LC–MS/MS; covers 25 PFAS including short-chain compounds in drinking water.</li><li><a target="_blank" rel="noopener noreferrer" href="https://blog.organomation.com/blog/sample-concentration-epa-method-537-pfas-determination">EPA Method 537.1</a>: Reversed-phase SPE followed by LC–MS/MS; covers 18 PFAS including the GenX compound, with method detection limits in the low ng/L range.</li><li><a target="_blank" rel="noopener noreferrer" href="https://www.agilent.com/en/solutions/environmental/water-testing/pfas-in-water/us-epa-method-8327?srsltid=AfmBOooCY1pcavj7B1PH-75d3HO4BGDglNlhmyTyrpDY3zvUksMO1dlg">EPA Method 8327</a>: LC–MS/MS for selected PFAS in prepared extracts of aqueous and solid matrices.</li><li><a target="_blank" rel="noopener noreferrer" href="https://blog.organomation.com/blog/preparing-diverse-sample-matrices-for-epa-method-1633">EPA Draft Method 1633</a>: The emerging benchmark for PFAS in non-aqueous matrices—aqueous, solid, biosolids, and tissue—quantifying up to 40 PFAS.</li></ul>All of these methods designate LC–MS/MS, specifically LC coupled to triple quadrupole or tandem-in-time platforms operating in negative electrospray ionization mode with multiple reaction monitoring (MRM), as the required or recommended analytical technique. PFAS analysis is, at its core, an LC–MS/MS problem.<h4>The Sample Preparation Bottleneck</h4>Chemists who have worked with PFAS methods understand the particular demands they place on sample preparation. The ubiquity of<a target="_blank" rel="noopener noreferrer" href="https://blog.organomation.com/blog/tackling-pfas-contamination-practical-insights-from-epa-537.1-method-development"> PFAS in laboratory materials</a>—PTFE tubing, some SPE sorbents, standard bench supplies—means that blank contamination control is as analytically demanding as the detection itself. EPA 537.1 requires that sample volumes of approximately 250 mL be extracted and reconstituted in as little as 1 mL, creating a 250:1 concentration factor that can expose even trace contamination in the sample processing chain. The installation of a delay column between the LC pump and autosampler to prevent instrument background interference is now standard practice.This is precisely where robust, <a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/article/5385">inert nitrogen evaporation </a>systems earn their keep. PFAS workflows that use nitrogen-assisted evaporation to concentrate extracts prior to reconstitution benefit from materials that avoid PTFE and other fluoropolymers that can leach PFAS background—a consideration Organomation addresses in its concentration system designs.The analytical community is also moving beyond targeted PFAS analysis. High-resolution accurate mass (HRAM) spectrometry via Orbitrap technology, paired with non-targeted screening workflows, is gaining traction for the identification of the more than 5,000 currently catalogued PFAS compounds and the novel structures that continue to emerge. LC–MS/MS remains the quantitative gold standard; HRAM provides the discovery layer.<h3>Technological Trends Reshaping the LC–MS/MS Workflow</h3><h4>Artificial Intelligence and Automated Data Interpretation</h4>The datasets generated by modern tandem MS platforms—particularly in omics applications—are too large and too complex for conventional manual review. AI-driven spectral deconvolution, automated peak integration, and AI-assisted hypothesis generation are now embedded in the software suites accompanying leading instruments. <a target="_blank" rel="noopener noreferrer" href="https://www.agilent.com/about/newsroom/presrel/2022/17feb-gp22004.html?srsltid=AfmBOop3warRIaEojXM2YyMML6RXfMIDzE-Wm0h-SiKkkocBeeET2m7u">Agilent’s acquisition of Virtual Control’s ACIES </a>software specifically to automate LC–MS and GC–MS data analysis is emblematic of this direction.For laboratory managers, AI integration means smaller specialized teams can operate high-throughput multi-analyte workflows—but it also means that the front-end sample preparation must be even more reproducible, since algorithmic interpretation amplifies inconsistencies introduced upstream.<h4>Online SPE Coupling and “Chromatography Without Walls”</h4>A significant trend is the integration of solid-phase extraction directly into the HPLC flow path—so-called online SPE–HPLC coupling. Systems that combine extraction and chromatographic separation in a single analytical instrument reduce manual transfer steps, minimize the risk of sample loss or contamination, and dramatically increase throughput. For analytes at very low concentrations, online SPE provides the on-column concentration capability that traditional offline preparation achieves with manual evaporation steps.That said, offline sample preparation—including <a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/article/5392">nitrogen-assisted evaporation</a> under controlled conditions, <a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/products/2407">liquid–liquid extraction</a>, and protein precipitation—retains significant advantages in flexibility, matrix handling, and the ability to process sample volumes that exceed online system capacity. The two approaches are increasingly complementary rather than competitive in well-designed analytical laboratories.<h4>High-Purity Solvents and Reference Materials</h4>As detection limits push further into the femtogram range and regulatory methods demand demonstrable blank performance, the quality of analytical consumables—mobile phase solvents, derivatization reagents, reference standards—has moved from background consideration to front-of-mind. Method validation in regulated environments now routinely requires vendor certification of solvent purity at a level that directly interfaces with instrument performance.<h3>The Ever-Present Need for Sample Evaporation</h3>As LC–MS/MS methods push detection limits lower, the analytical burden shifts increasingly upstream. The quality of the concentration, cleanup, and reconstitution steps preceding injection determines whether the instrument’s theoretical sensitivity is achievable in practice.Several specific developments are particularly relevant:<ul><li>ADC and gene therapy characterization workflows require gentle, low-temperature evaporation that preserves protein structure and prevents aggregation prior to LC–MS/MS. Nitrogen evaporation under controlled conditions is the preferred approach.</li><li>PFAS trace analysis demands evaporation systems constructed from PFAS-inert materials, with rigorous attention to avoiding fluoropolymer surfaces throughout the sample path.</li><li>High-throughput pharmaceutical QC driven by reshoring demands parallel processing capability—multi-position evaporators that maintain consistent flow and temperature across all positions simultaneously.</li><li>Multi-omics and metabolomics workflows generate large sample batches that benefit from automated, walk-away evaporation with programmable endpoints to ensure reproducible final volumes across a cohort.</li></ul>Tandem chromatography–mass spectrometry is not merely growing; it is becoming structurally embedded in the analytical workflows of every major regulated industry. The convergence of biopharma complexity, PFAS regulatory mandates, AI-enabled data analysis, and pharmaceutical reshoring is creating a multi-year demand cycle that will require laboratories to invest not only in instruments, but in the entire sample preparation ecosystem that makes those instruments perform.For analytical chemists, the practical takeaway is this: as mass spectrometry sensitivity continues to advance, the sample preparation step becomes the binding constraint on method performance. Whether you are developing a new PFAS method to meet EPA 1633, characterizing an ADC’s drug-to-antibody ratio, or running a 500-sample metabolomics cohort overnight, the reliability and cleanliness of your concentration and cleanup workflow determines the quality of every data point that follows.&nbsp;

The Continued Rise of Tandem Chromatography–Mass Spectrometry

<h3>A Practical Guide to Getting the Most from Syringe Filters: Tips, Tricks, and Common Pitfalls</h3>Syringe filters play a crucial role in chromatography and many other laboratory applications by removing particulate matter before analysis. Proper sample filtration helps protect analytical instruments, extends column lifetime, and improves data quality. However, selecting an unsuitable filter—or using one incorrectly—can introduce contamination, reduce filtration efficiency, or even compromise analytical results. The following recommendations will help you avoid common mistakes and ensure reliable filtration every time.<img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/KNAUER_Avoiding_Common_Syringe_Filter_Mistakes_3_150e8c1c2b.webp" alt="KNAUER: Avoiding Common Syringe Filter Mistakes" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_KNAUER_Avoiding_Common_Syringe_Filter_Mistakes_3_150e8c1c2b.webp 133w," sizes="100vw" width="1276" height="1496"><h4>1. Improper Filter Handling</h4>Using excessive force during filtration or attempting to reuse disposable syringe filters can damage the membrane and increase the risk of sample contamination.<ul><li>Solution: Apply slow, even pressure throughout the filtration process and always dispose of single-use filters after one use. If the flow rate decreases noticeably, replace the filter instead of applying additional force.</li></ul><h4>2. Selecting an Inappropriate Pore Size</h4>Using the wrong pore size may either fail to remove unwanted particles or cause the membrane to clog prematurely, reducing filtration efficiency.<ul><li>Solution: A 0.45 µm membrane is generally suitable for routine particulate removal, while a 0.22 µm membrane is recommended for finer filtration and bacterial removal. Samples with higher viscosity may require a pre-filter to prevent rapid clogging. It is also important to consider the intended analytical technique. For example, 0.22 µm filters are typically preferred for UHPLC and LC-MS applications, whereas 0.45 µm filters are often sufficient for conventional HPLC analyses.</li></ul><h4>3. Choosing the Wrong Membrane Material</h4>Different membrane materials exhibit different chemical resistance. Using an incompatible membrane may lead to degradation of the filter, sample contamination, or complete filtration failure.<ul><li>Solution: Always verify solvent compatibility before filtration. PTFE membranes are an excellent choice for organic solvents, while PES and nylon membranes are better suited to aqueous samples. If compatibility is uncertain, perform a small-scale trial before filtering valuable samples.</li><li>Pro Tip: For additional guidance, see our blog article "How to Choose the Right Membrane for your Syringe Filter", which provides a detailed comparison of membrane materials and their recommended applications.</li></ul><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/KNAUER_Avoiding_Common_Syringe_Filter_Mistakes_2_e47021fb11.jpg" alt="KNAUER Sepapure® Syringe Filters are available with multiple membrane materials, each identified by a dedicated color code for quick and reliable selection." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_KNAUER_Avoiding_Common_Syringe_Filter_Mistakes_2_e47021fb11.jpg 245w," sizes="100vw" width="1580" height="902"><h4>4. Ignoring Filter Capacity</h4>Using a filter with insufficient capacity can result in rapid membrane blockage, increasing filtration time and reducing laboratory productivity.<ul><li>Solution: When filtering larger sample volumes, choose high-capacity syringe filters or introduce a pre-filtration step. Never force liquid through a clogged membrane, as doing so may damage the filter and negatively affect sample quality.</li></ul><h4>5. Overlooking Sample Adsorption</h4>Certain membrane materials may adsorb analytes of interest, leading to lower recoveries and potentially affecting quantitative results.<ul><li>Solution: For protein samples or other adsorption-sensitive analytes, select membranes with low binding characteristics, such as nylon or glass fiber, whenever appropriate. Performing a small recovery test before routine analysis can help identify potential analyte losses.</li></ul><h4>6. Failing to Optimize Filtration Performance</h4>Slow filtration is more than just an inconvenience—it often indicates that the filtration conditions are not optimal.<ul><li>Solution: If filtration becomes unusually slow, investigate possible causes such as membrane clogging, excessive sample viscosity, or an unnecessarily small pore size. Depending on the sample, dilution or sequential filtration using progressively finer filters may significantly improve performance.</li></ul><h4>Final Thoughts</h4>Selecting the appropriate syringe filter is a straightforward process, but overlooking small details can have a significant impact on analytical performance. Choosing the correct membrane material, pore size, and filtration strategy helps improve laboratory efficiency, protects chromatography systems and columns, and contributes to accurate, reproducible analytical results.With the right syringe filter and proper filtration technique, you can minimize sample preparation problems and maximize confidence in every analysis.<img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/KNAUER_Avoiding_Common_Syringe_Filter_Mistakes_4_983a15e289.jpg" alt="KNAUER: Sepapure® Syringe Filter Selection" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_KNAUER_Avoiding_Common_Syringe_Filter_Mistakes_4_983a15e289.jpg 245w," sizes="100vw" width="1160" height="627">Happy filtering!

Avoiding Common Syringe Filter Mistakes

News from LabRulezLCMS Library - Week 27, 2026

Article

Product

Application

Academy

Poster

Everything from the world of analytical chemistry in one place. We connect people in solving their problems. At Labrulez you will find all the necessary information easily, quickly and clearly. Stop searching and start finding.

LabRulez

Organomation, founded in 1959, designs and manufactures high-quality nitrogen evaporators and extraction systems. Known for innovation and durability, their lab instruments are used globally for efficient sample preparation with strong customer support.

Organomation

Based in Berlin, KNAUER is a medium-sized, owner-managed company that has been serving the sciences since 1962. We develop and manufacture scientific instruments of superior quality for liquid chromatography, including: Analytical HPLC/UHPLC, Preparative HPLC, Fast protein liquid chromatography (FPLC), Multi-column chromatography/Simulated moving bed (SMB) chromatography. High pressure dosing, in-line detection systems, and osmometry are additional business areas.

KNAUER

The Analytical Scientist

Agilent Technologies

Phenomenex

Thermo Fisher Scientific

Shimadzu Corporation

Waters Corporation

<ul>
<li>Wait what?! Who knew!? There are so many things that a mass spec can do.
</li>
<li>I&#39;ve used it for years, yes this may be true.
</li>
<li>I&#39;ve processed some samples, more than a few.
</li>
<li>But between me and you, I had no idea all the things it could do!
</li>
<li>From sample prep to separation, you just won&#39;t believe…
</li>
<li>All of the tips and tricks we have up our sleeve.
</li>
<li>So, take a few minutes, about 15 should do.
</li>
<li>And learn all the things your mass spec can do.
</li>
</ul>
Thinking of developing a new method utilizing MS? Selecting the best analytical method begins with a decision on how to best separate the analytes of interest. This choice will affect sample preparation and sample introduction, so it is important to get it right early. In this webinar we will review analyte structural properties that make the compound more amenable to either liquid chromatography or to gas chromatography.
<blockquote>
Presenter: Karen Yannell (LC/MS Application Engineer, Agilent Technologies, Inc.)
</blockquote>
<blockquote>
Karen E. Yannell is a LC/MS Application Engineer at Agilent Technologies. She is an expert in small molecule high throughput analysis by triple quadrupole (TQ) and quadrupole time-of-flight (QTOF) mass spectrometry for applied markets. Her recent work has focused on developing routine Q-TOF workflows for both expert and non-expert users alike. Previously, Karen worked on forensic, metabolomics, and biomarker discovery applications with ambient ionization sources. She received her PhD in Analytical Chemistry from Purdue University where studied under the advisement of Prof. R. Graham Cooks. Outside of the laboratory, she enjoys cooking unique dishes and hiking along the beach with her dog.
</blockquote>
<blockquote>
Presenter: David Weil, PhD (Senior Applications Scientist, Agilent Technologies, Inc.)
</blockquote>
<blockquote>
David Weil is a senior applications scientist in Agilent located in Wood Dale with over 30 years of experience using mass spectrometry. Prior to joining Agilent in 2004, he worked for 14 years at 3M in the Corporate Research Analytical Laboratories (St.Paul, MN) and 4 years at Nicolet/Extrel doing ultra-high resolution mass spectrometry. David received his Ph.D. in Physical Chemistry from the University of Minnesota and Post Doc with Dr Charles Wilkins at University of California-Riverside developing laser desorption FTMS and ion-molecule reactions. David has three grown sons and live with his wife Naomi just blocks from Wrigley Field and Lake Michigan.
</blockquote>
<blockquote>
Presenter: Matt Curtis, PhD (GC/MS Applications Scientist, Agilent Technologies, Inc.)
</blockquote>
<blockquote>
Matt joined Agilent in 2013 as a GC/MS applications scientist in the Santa Clara, CA Center of Excellence. His primary responsibility is focused on providing instrument demos for customer interested in each technology Agilent offers; the GC/MS single quadrupole, triple quadrupole and the quadrupole Time-of-Flight. The variety of different samples submitted to the demo lab provide a unique knowledge of optimizing parameters for different applications to provide the best quality data from the instrument.
</blockquote>

Agilent Technologies: Selecting your Separation: Are my Compounds Better for GC or LC

Selecting your Separation: Are my Compounds Better for GC or LC

We will review analyte structural properties that make the compound more amenable to either liquid chromatography or to gas chromatography.

Separation selection: Are my Compounds Better for GC or LC

Interactions of analytes with metal surfaces in high-performance liquid chromatography (HPLC) instruments and columns have been reported to cause deleterious effects ranging from peak tailing to a complete loss of the analyte signal. These effects are due to the adsorption of certain analytes on the metal oxide layer on the surface of the metal components.
We have developed a novel surface modification technology and applied it to the metal components in ultra HPLC (UHPLC) instruments and columns to mitigate these interactions. A hybrid organic−inorganic surface, based on an ethylene-bridged siloxane chemistry, was developed for use with reversed-phase and hydrophilic interaction chromatography. We have characterized the performance of UHPLC instruments and columns that incorporate this surface technology and compared the results with those obtained using their conventional counterparts. 
We demonstrate improved performance when using the hybrid surface technology for separations of various small molecules as well as nucleotides, phosphopeptides, and oligonucleotides. The hybrid surface technology was found to result in higher and more consistent analyte peak areas and improved peak shape, particularly when using low analyte mass loads and acidic mobile phases. Reduced abundances of iron adducts in the mass spectrum of a peptide were also observed when using UHPLC systems and columns that incorporate hybrid surface technology. These results suggest that this technology will be particularly beneficial in UHPLC/mass spectrometry investigations of metal-sensitive analytes.
Join this session to:
<ul>
<li>Learn about non-specific adsorption and the challenges it can cause</li>
<li>Learn about current workarounds that scientists currently use to mitigate non-specific adsorption</li>
<li>Learn about MaxPeak HPS technology and how this prevent non-specific adsorption across many different workflows</li>
</ul>
<blockquote>
Presenter: Kim Haynes (Director of Chemistry Marketing and Product Management , Waters Corporation)
</blockquote>
<blockquote>
Kim Haynes is a Director of Chemistry Marketing and Product Management in the Consumables and Lab Automation Group at Waters Corporation. Kim manages MaxPeak High Performance Surface Technologies as well as a variety of other programs. Prior to this position, she has worked with Waters Corporation as a chemistry sales specialist and a business development manager.
</blockquote>
<blockquote>
During her 23 years at Waters, Kim has focused on helping scientists and colleagues understand chromatographic separations and sample preparation. Before joining Waters Corporation, Kim worked as an analytical chemist at Bacardi Martini. She holds a BS in Chemistry from Ohio University.
</blockquote>

The Analytical Scientist: Increasing method reproducibility by eliminating unwanted analyze surfaced interactions

Increasing method reproducibility by eliminating unwanted analyze surfaced interactions

Performance when using the hybrid surface technology for separations of various small molecules as well as nucleotides, phosphopeptides, and oligonucleotides. 

Reproducibility & eliminating unwanted surfaced interactions

WEOF is back for 2023, and this time we will do it 100% virtually to allow even more people to participate. But don&#39;t expect to see only some presentations! We have put together for you an interesting agenda with Live Demos, customer talks, and the chance to ask our experts your questions. It will be a great opportunity to learn more about the advancements in oligonucleotide analysis and about the analytical challenges and successes of your peers.
<ul>
<li>Join our virtual Live Demos</li>
<li>Hear from other scientists like you about their approaches and experiences</li>
<li>Stay up to date with the latest analytical developments for nucleic acids</li>
</ul>
The agenda will be a mix of talks from the scientific community and Waters presentations. We will discuss current analytical needs and show some Live Demos of LC/MS solutions and software:
<h3>AGENDA</h3>
<h5>10:00 Welcome and Short Introduction</h5>
<ul>
<li>Speaker: David Muñoz (Waters Corporation)</li>
</ul>
<h5>10:05 Waters Live Demo: Impurity Analysis by LC/MS</h5>
<ul>
<li>Speaker: Emma L Harry (Waters Corporation)</li>
</ul>
<h5>10:25 Customer Talk: Cytiva Sweden AB</h5>
<h5>Analysis of Oligonucleotides using BioAccord LC-MS</h5>
<ul>
<li>Speaker: Filip Bergqvist (Senior Scientist)</li>
</ul>
Topic: intact analysis of oligonucleotides, specifically development/performance with IP-RPC
<h5>10:40 Customer Talk: BioNTech Manufacturing GmbH</h5>
<h5>QC Analytics for mRNA therapies</h5>
<ul>
<li>Speaker: Jonas Sander</li>
</ul>
Topic: How BioNTech has masterdto set up QC analytics for mRNQ therapies and the added value of Waters solutions
<h5>10:55 MaxPeak Premier Technology for Oligonucleotide Analysis</h5>
<ul>
<li>Speaker: Ali Tiss (Waters Corporation)</li>
</ul>
<h5>11:10 Waters Live Demo: Software</h5>
<h5>CONFIRM &amp; INTACT app</h5>
<ul>
<li>Speaker: Chris Knowles (Waters Corporation)</li>
</ul>
<h5>11:30 Q&amp;A with Waters</h5>
You ask - our application chemists answer
<h5>11:45 Closing</h5>
<h4>INVITED GUEST SPEAKER</h4>
<h5>Filip Bergqvist (Senior Scientist, Cytiva Sweden AB)</h5>
Filip is leading bioanalytical activities to support product development and application work for bioprocessing. His main expertise is LC-MS, where he is developing methods for characterization of proteins and nucleic acid therapeutics. 
<h5>Dr. Jonas Sander (BioNTech Manufacturing GmbH)</h5>
(further info will follow soon)
<h4>YOUR WATERS HOSTS</h4>
<h5>David Muñoz (Principal Biopharma Market Development Manager, Waters Corporation)</h5>
David will be your host for this event. He completed his PhD in Biochemistry at Nottingham Trent University, focused on the effects of toxins on glioma cells. After this he joined Lonza to work as a research and development scientist using different analytical techniques to characterize antibodies and proteins. Later he worked for Thermo Fisher Scientific as applications specialist and Biopharma Expert, and as business development manager in two CDMOs, focusing on antibodies and viral vector projects. David joined Waters in June 2022 as principal biopharma market development manager. 
<h5>Emma L Harry (European Applications Laboratory Team Manager, Waters Corporation)</h5>
Emma completed her PhD in Analytical Chemistry at Loughborough University, focused on the development of ion mobility mass spectrometry for complex mixtures.
After her post doctoral research at University of Leicester and having worked as analytical scientist at Hall Analytical, using a variety of analytical techniques, Emma joined Waters in June 2019 as a senior applications chemist in the Demo lab in Wilmslow. In 2021 she was promoted to European applications lab manager.
<h5>Chris Knowles (Senior Applications Scientist, Waters Corporation)</h5>
Chris is currently working as a Principal Domain Specialist (Applications science) at Waters, having been with the company for 5 years. He works with the Waters informatics teams to help deliver the waters_connect software applications. His focus has been on the CONFIRM Sequence application. His background is in Bioprocessing, having worked at FUJIFILM Diasynth Biotechnologies for 3 years as a principal scientist in Mammalian cell culture, following completion of a PhD in mammalian cell line engineering at the University of Aberdeen in 2016.
<h5>Ali Tiss (Sales Specialist Biopharma Chemistry, Europe South, Waters Corporation)</h5>
Ali completed his Ph.D. in biochemistry and structural biology at the Mediterranean University (CNRS, Marseille, France), where he contributed to the R&amp;D of the anti-obesity drug, Xenical®, developed by Roche Ltd. Then, he spent about 20 years of academic research (France, UK, Kuwait) in biochemistry, liquid chromatography, mass-spectrometry, and proteomics, focusing on detecting and characterizing biomarkers related to human diseases (cancer, diabetes, obesity). Ali joined Waters in October 2021 as a BioPharma and New Modalities Specialist in Chemistry for Southern Europe.

Waters Corporation: Waters European Oligonucleotides Forum 2023

Waters European Oligonucleotides Forum 2023

We have put together for you an interesting agenda with Live Demos, customer talks, and the chance to ask our experts your questions. 

There is a lot of interest in adeno-associated viruses (AAVs), especially as vectors for gene therapy. AAVs are good candidates for this application due to their ability to infect both dividing and non-dividing cells without eliciting a strong immune response. Before AAV products can be released, they require unambiguous identification.
Agilent delivers total solutions for efficient AAV characterization and analysis while monitoring critical quality attributes.
This virtual event will provide you with expertise on:
<ul>
<li>LC/MS workflows for the identification of AAV capsid proteins and impurities</li>
<li>SEC workflows for aggregate and fragment analysis</li>
<li>IEX workflows to determine empty/full capsid ratios</li>
</ul>
Confirmed presentations
<ul>
<li>Introduction to the Analysis of Adeno-associated Virus Particles</li>
<li>AAV Capsid Protein Analysis using high resolution LC/MS</li>
<li>Multiple Peptide-Based Attributes of AAV with a Comprehensive LC/MS Method</li>
<li>Analytics of AAV based Gene Therapeutics</li>
</ul>
Who should attend?
<ul>
<li>Analytical development scientists</li>
<li>CDMO and CRO lab personnel</li>
<li>Biopharmaceutical scientists in process development</li>
</ul>

Agilent Technologies: Agilent AAV Workflow Summit: From Identity Confirmation to Aggregate Analysis

Agilent AAV Workflow Summit: From Identity Confirmation to Aggregate Analysis

Join us for half a day of virtual presentations on AAV analytical workflows

Agilent AAV Workflow Summit: From Confirmation to Analysis

This meeting covers the latest trends and techniques to meet current water and environmental testing demands for emerging contaminants and evolving regulations. Our guest speakers from the water industry and environmental research and testing laboratories will share their expertise on their latest work, analytical workflows, and automation.
Join us and discover valuable insights to maximise testing productivity and instrument uptime, simplify workflows and standardise data workup.
<h3>June 28 (Day 2) – Evolving regulations</h3>
This session will explore disinfection by-products, haloacetic acids, heavy metals, volatiles/semi-volatiles analysis.
Attend the whole day or selected presentations only.
<h5>10:00 Online access opens (Streaming begins 09:30 BST/10:30 CEST)</h5>
<h5>10:30 Opening of the event, welcome and introduction</h5>
<ul>
<li>Presenter: Ed George (Thermo Fisher Scientific)</li>
</ul>
<h5>10:50 Beyond compliance - use of the Orbitrap Exploris 120 HRAM-LC-MS for the monitoring of catchments</h5>
<ul>
<li>Presenter: Mark Collins (Northern Ireland Water)</li>
</ul>
<h5>11:20 Utilizing IC-MS/MS for the analysis of halo acetic acids and disinfection by products in drinking water</h5>
<ul>
<li>Presenter: Chris Pegg (Anglian Water)</li>
</ul>
<h5>11:50 GC-MS analysis of environmental samples using automated sample preparation</h5>
<ul>
<li>Presenter: John Quick (ALS Environmental Ltd.)</li>
</ul>
<h5>12:20 Break</h5>
<h5>12:40 Development of an ion chromatography method with EC and MS detection for the analysis of bromide and the disinfection by products bromate, chlorate and chlorite</h5>
<ul>
<li>Presenter: Joseph Scott (Affinity Water)</li>
</ul>
<h5>13:10 High throughput nutrients analysis - developing methodologies for environmental and wastewater matrices</h5>
<ul>
<li>Presenter: Red Godwin (Thermo Fisher Scientific)</li>
</ul>
<h5>13:40 Performing the analysis of trace metals in environmental samples via ICP and ICP-MS</h5>
<ul>
<li>Presenter: Trudy McMurray (Northern Ireland Environment Agency)</li>
</ul>
<h5>14:15 Closing of the live streaming event</h5>

Thermo Scientific: Water & Environmental Analysis User Meeting - Day 2

Water & Environmental Analysis User Meeting - Day 2

Streaming live from our Demo Centre in Hemel Hempstead | Join the complete event or the presentations of your choice only.

As a continuation of our Chromatography of Bioseparations 101 series, we bring to you 2 NEW SESSIONS in our Chromatography of Bioseparations 102 webinar series! Join us live for our new parts 6 and 7. If you haven&#39;t had an opportunity to register for Parts 1-5, feel free to regster and listen to them on-demand now.
These new webinars will focus on the analytical separation of biomolecules such as peptides and amino acids.
Reverse-phase chromatography is the predominant technique used for the analyses and or purification of chemically synthesized peptides or peptides generated by the enzymatic digestion of proteins (i.e., peptide mapping). It is advantageous to have a simplified step-by-step approach to develop a method that can be applied to chemically synthesized peptides or protein digests.
Amino acid analysis is required in several different areas of research and is a fundamental tool in various product analytical activities. Due to the varied properties of amino acids (i.e., acidic, basic, etc.), the separation and detection represents an analytical challenge and depending on throughput, sample preparation can be laborious. 
Who should attend?
<ul>
<li>Those interested in a refresher on foundational principles and tips of reverse phase separations of peptides.</li>
<li>Those interested in how to select columns and mobile phases that are optimal for peptide and amino acid workflows.</li>
<li>Those looking for an overview of industry advancements and new offerings.</li>
</ul>
<h3>PART 7 | June 27th, 2023 | 10:30AM – 12:00PM EDT</h3>
<h3>UNDERSTANDING AMINO ACID ANALYSIS FOR PROTEIN HYDROLYSATES AND CELL CULTURE MEDIA</h3>
<ul>
<li>Understand the challenges of sample preparation, separation, and detection of amino acids in different matrices.</li>
<li>Review analytical techniques that addresses the challenges of amino acid analysis.</li>
</ul>
<blockquote>
Presenter: Paula Phenix (Principal Product Manager – Consumables and Lab Automation, Waters Corporation)
</blockquote>
<blockquote>
Paula is a Principal Marketing Manager at Waters with a focus on Waters products for amino acid and glycan analysis. Prior to her current role in the Chemistry and Lab Automation Group, she was the Product Marketing Manager for Specialty LC and SFC Systems in the Instruments group. Before joining Waters, she held product management, marketing and sales roles for several companies (Life Technologies, Promega, MilliporeSigma) in the molecular biology, immunology, cell culture and single-use bioprocessing areas. Paula graduated from the University of Delaware with a Bachelor’s Degree in Biology and from Johns Hopkins University with a Masters Degree in Business.
</blockquote>

UNDERSTANDING AMINO ACID ANALYSIS FOR PROTEIN HYDROLYSATES AND CELL CULTURE MEDIA

Understand the challenges of sample preparation, separation, and detection of amino acids in different matrices.

UNDERSTANDING AMINO ACID ANALYSIS

This webinar introduces an analytical method that utilizes ion-exchange chromatography to separate and analyze oligonucleotides of different lengths. The primary focus is on eliminating impurities that are produced during the synthesis process.
The method incorporates an inert ultra-high-performance liquid chromatography system, liquid chromatography-tandem mass spectrometry, and automated data analysis software. These components work together to facilitate efficient oligonucleotide development and quality control. The results emphasize the challenges associated with metal-based methods and propose a metal-free approach for precise quantification. Additionally, a high-resolution ion-pair reversed-phase LC-MS system is presented for accurate separation and mass analysis of oligonucleotides. The developed method also offers sensitive, precise, and reproducible results, making it an invaluable tool for various scientific fields, including therapeutic oligonucleotide-based drug development and quality control.
<blockquote>
Speaker: Mridul Mandal, Ph.D.
</blockquote>
<blockquote>
Marketing Manager, Shimadzu Scientific Instruments Mridul Mandal, Ph.D. is currently working as a market manager -pharma/biopharma at Shimadzu Scientific Instruments. Before entering this position, he held numerous roles in academia and pharma CRO for over 12 years. He has published over 30 peer-reviewed publications and book chapters on mass spectrometry, chromatography, and other instrumentation in biomedical science
</blockquote>

Shimadzu: Oligonucleotide Analysis by LC and LC-MS

Oligonucleotide Analysis by LC and LC-MS

This webinar introduces an analytical method that utilizes ion-exchange chromatography to separate and analyze oligonucleotides of different lengths.

Purification is critical to many scientific workflows. Compounds of interest are rarely found to be the right purity in their native state. Join Waters and Virscidian for this 6-part webinar series to learn the basics of purification and application of purification principles for more advanced workflows.
<h3>PART 4 | Using the Waters Fraction Manager Analytical (WFMA) to Achieve UPLC Scale Purification</h3>
Analytical scale purification provides for routine and robust isolation of your investigatory compounds. Challenges with UPLC purification include peak widths of 1-3 seconds and collecting the 6 to 50 µL peak volume. The Waters Fraction Manager Analytical (WFMA) addresses these challenges. Learn more about analytical scale purification with Waters. 
Learning Objectives: 
<ul>
<li>Understand analytical scale purification</li>
<li>Implement best practices to collect small volume fractions</li>
</ul>
<blockquote>
Presenter: Andrew Leightner (Market Development Manager – Americas Field Marketing, Waters Corporation)
</blockquote>
<blockquote>
Andy Leightner has over 10 years of experience serving the pharma market from contract research organizations. He specialized in method development and validation leveraging LC, GC, and mass spectrometry to meet complex client needs. He supports pharmaceutical and biopharmaceutical discovery and development in the Americas for Waters Corporation. Andy received a bachelors degree in biology from the University of Wisconsin-Madison.
</blockquote>

Waters Corporation: Using the Waters Fraction Manager Analytical (WFMA) to Achieve UPLC Scale Purification

Using the Waters Fraction Manager Analytical (WFMA) to Achieve UPLC Scale Purification

Learn more about analytical scale purification with Waters.

Waters Fraction Manager Analytical UPLC Scale Purification

Key Learning Points:
<ul>
<li>Gain a comprehensive understanding of solvent miscibility and recognize its significance
</li>
<li>Develop an in-depth comprehension of how to properly solvent switch a column, and the potential consequences associated with incorrect procedures
</li>
<li>Familiarize yourself with a standardized protocol for transitioning an LC column from one solvent system to another, ensuring a seamless solvent switch
</li>
</ul>
Who Should Attend:
Any LC users looking to utilize different modes of separation and are required to switch a column that is shipped with an immiscible solvent to the one method requires
<blockquote>
Presenter: Genevieve Hodson (Technical Support, Phenomenex)
</blockquote>

Phonomenex: How to Solvent Switch Successfully

How to Solvent Switch Successfully

Gain a comprehensive understanding of solvent miscibility and recognize its significance.

High performance liquid chromatography (HPLC) is a commonly used technique in the pharmaceutical research and manufacturing sectors to determine the concentration and purity of active pharmaceutical ingredients (APIs) or to measure process related residuals and formulation components. In biopharmaceuticals manufacturing or formulation, polysorbates or poloxamers are commonly present either as process related residuals or as formulation components.
The detection and quantitation of these compounds can be a challenge due to their lack of a chromophore which makes traditional UV detection impossible and interference from proteinaceous APIs. This presentation reviews the development and validation strategies of a robust method for the quantitation of poloxamer P188 in biopharmaceuticals. The method uses charged aerosol detection (CAD) on the Thermo Vanquish Flex HPLC system and has suitable sensitivity to quantitate poloxamer P188 down to 3 µg/mL in both AAV and mAb APIs. 
The method is specific in that it is capable of quantitating poloxamer P188 in common formulations and demonstrates non-interference from polysorbate-80 and PEI.
Join our webinar on Tuesday, July 11th at 15:00 CEST (14:00 GMT, 06:00 PDT, 09:00 EDT) to hear about the work of our guest speaker, Adam Punke, from PPD, Part of Thermo Fisher Scientific. In his presentation, he will share his experience of the development and validation strategies of a robust method for the quantitation of poloxamer P188 in biopharmaceuticals.
<h5>Learning points:</h5>
<ul>
<li>What makes charged aerosol detection so powerful</li>
<li>What benefits it offers</li>
<li>What applications are ideal for charged aerosol detection?</li>
</ul>
<h5>Who should attend:</h5>
<ul>
<li>Laboratory managers and directors</li>
<li>Technology leaders</li>
<li>Principal scientists</li>
<li>Laboratory analysts</li>
</ul>
<blockquote>
Presenter: Adam Punke (Guest Speaker, Sr. Research Scientist, PPD, Part of Thermo Fisher Scientific)
</blockquote>
<blockquote>
Presenter: Chris Tuczemskyi (Moderator, Product Marketing Manager, Thermo Fisher Scientific)
</blockquote>
<blockquote>
Chris Tuczemskyi is a chromatography specialist at Thermo Fisher Scientific, based in Cambridge, UK. After spending a number of years building up his experience with chromatography instruments in a water testing laboratory, he joined Thermo Fisher Scientific in 2014 as a chromatography service engineer for Unity Lab Services. Here he developed his passion for supporting customers using HPLC and in 2018 moved into the application specialist team. More recently, in 2022 he made the move to Product Marketing to help ensure we meet and exceed our customers expectation. In 2009, Chris graduated from the University of Essex in Biochemistry (Bsc Hons).
</blockquote>
<blockquote>
Presenter: Ian Acworth (Analytical Instruments Group, Thermo Fisher Scientific)
</blockquote>
<blockquote>
Ian Acworth is part of the marketing team at Thermo Fisher Scientific, based near Boston, Massachusetts. He directed application chemistry development for several HPLC companies while ensuring a strong focus on customer needs and support. He was part of the team that developed and supported the Charged Aerosol Detector. Ian completed his D. Phil in biochemistry at Oxford University in 1986 and completed his postdoctoral studies in neuroscience at MIT in 1989.
</blockquote>
<blockquote>
Presenter: Frank Steiner (Senior Manager of Product Applications, Thermo Fisher Scientific)
</blockquote>
<blockquote>
Frank Steiner is Senior Manager of Product Applications and coordinates HPLC based scientific collaborations in Thermo Fisher Scientific as scientific advisor. He joined Dionex Corporation in 2005, now a part of Thermo Fisher Scientific, and had been manager in various HPLC marketing functions. Frank received his PhD degree in chemistry in 1995 from Saarland University in Saarbruecken, Germany where he became assistant professor in 1997 and associate professor in 2003, after his postdoc at the nuclear research center in Saclay, France in 1996. Frank published more than 30 publications in refereed journals and more than 10 text book chapters on HPLC topics.
</blockquote>
<blockquote>
Presenter: Katherine Lovejoy (Product Application Specialist, Thermo Fisher Scientific)
</blockquote>
<blockquote>
Katherine Lovejoy, Ph.D., has been an application chemist focusing on charged aerosol detection at the Thermo Fisher Scientific HPLC factory site since 2016 and has a Ph.D. in inorganic chemistry from the Massachusetts Institute of Technology. She has experience as a chemist reviewer at the FDA and as a scientist at Los Alamos National Laboratory.
</blockquote>

Thermo Scientific: Quantitation of Poloxamer P188 in Biopharmaceutical Formulations Using Charged Aerosol Detection

Quantitation of Poloxamer P188 in Biopharmaceutical Formulations Using Charged Aerosol Detection

We will share his experience of the development and validation strategies of a robust method for the quantitation of poloxamer P188 in biopharmaceuticals.

LCMS webinars focusing on HPLC - page 37