Professor Michaela Rumlová, head of a research group of the National Institute of Virology and Bacteriology at UCT Prague (and Vice-Rector for doctoral studies), holder of a licensed patent &nbsp;for antiretroviral testing assays, and recipient of the Czech Science Foundation’s President’s Award for HIV research, has also become the principal investigator of the bilateral INTER-EXCELLENCE II project “Cellular factors regulating the export and packaging of unspliced ​​retroviral RNA”, starting this year. In this interview, she explains how retroviruses such as HIV “hack” the cell in order to export their own genome from the nucleus into the cytoplasm, and then to the site where it is specifically packaged into nascent viral particles, a key step in viral replication. Her team is collaborating with the NIH (National Institutes of Health) and Rockefeller University to elucidate mechanisms that may help reveal new vulnerable points in the retrovirus replication cycle while simultaneously clarifying related cellular processes.<h5>Could you describe your project and its main objectives?</h5>Retroviruses, such as HIV, are viruses that carry genetic information in the form of RNA; however, after entering a cell, they are able to reverse transcribe it into DNA and integrate it into the host genome. In order to replicate, they must transport their genetic information—in the form of unspliced RNA—from the nucleus into the cytosol. There, it serves both as a template for the production of viral proteins and as the genome of new viral particles. This is highly unusual, as the cell very carefully controls which RNA molecules are allowed to leave the nucleus. Normal protein-coding cellular RNAs, known as messenger RNAs (mRNAs), must undergo splicing—a processing step in which certain segments are removed—before being exported. When this process fails, mRNA that retains these segments is held back in the nucleus as part of quality control and subsequently degraded. Retroviruses have managed to outwit this cellular control machinery, allowing their unspliced RNA to pass into the cytoplasm.Our project focuses on identifying the cellular proteins that assist retroviruses in this step and on the mechanism by which protein complexes bound to viral RNA are remodelled during RNA transport. The goal is to understand how the virus uses cellular mechanisms to its advantage and how this pathway is linked to the subsequent fate of the RNA, i.e. to its translation and packaging into new viral particles.<h5>Why is this export of unspliced ​​RNA so important for retroviral replication?</h5>Because this RNA has a dual role for the virus. On the one hand, it serves as a template for synthesizing viral proteins in the cytoplasm; on the other, it represents the genetic material itself, which must be packaged into new viral particles. The virus must package its RNA genome in its unspliced form, as only this RNA contains essential sequences without which further viral replication would be impossible.Simply put: the virus needs to get a complete copy of its genetic instructions out of the nucleus. Once the viral RNA is in the cytoplasm, it can be used as a template for synthesizing viral proteins; and a portion of these RNA molecules is then selected and packaged into a new particle as a genome. We are interested in how the virus forces the cell to allow viral RNA that is, from the cell’s perspective, “unfinished,” that is unspliced, to leave the nucleus. We are also investigating how the cell and the virus together - albeit unintentionally from the cell’s perspective - determine which RNA molecule will be translated into proteins and which will be packaged as the genome. This is one of the key, yet still not fully understood, steps of retroviral replication.<h5>What do you think it was about your project that impressed the Ministry of Education, Youth and Sports’ jury the most?</h5>I believe the decisive factor was a combination of several elements. First, the reviewers were primarily interested in the scientific problem itself. We are investigating a mechanism that is essential for retroviruses, but still not sufficiently understood, which may also have broader implications for the regulation of cellular processes. In addition, the quality of the team, our strong collaboration with the NIH and Rockefeller University, and the use of modern methods that will allow us to track viral RNA in great detail also played a role. It was also important that the project has clear objectives and a realistic plan.<h5>How will this research contribute to the development of new treatments for retroviral infections?</h5>It is important to say that this is primarily basic research. Rather than developing a new drug directly, we are trying to understand, at the molecular level, the step that the virus cannot do without. Only when we fully understand the mechanism of these cellular processes and identify the essential factors for the export of unspliced ​​RNA and how they function, will we be able to better determine which interactions or mechanisms represent potentially vulnerable points. Similar mechanisms associated with the export of unspliced ​​mRNA also appear in some diseases, such as cancer, so I &nbsp;see the benefit of our research mainly in that it will refine our understanding of these pathological processes in the cell, and retroviruses help us do so. Therefore, our work on retroviruses not only shows us how the virus works, but also how the cell itself works.<h5>What responsibilities does your UCT Prague team have? How does the collaboration with your American partners work?</h5>At UCT Prague, we are conducting the project’s primary experimental work. Our research involves cell cultures, viral models, and various biochemical techniques ranging from immunochemistry to microscopy that allow us to track viral RNA and identify its cellular partners. In collaboration with colleagues from NIH, Frederick, we are developing single-particle tracking methods to observe viral RNA movement in living cells across both space and time. Furthermore, we are working with colleagues from Rockefeller University to employ iCLIP methods for the precise mapping of protein-binding sites on RNA. Both collaborations are valuable to us, because they are not just about data sharing, but also about the transfer of expertise and detailed know-how.<h5>Who else is collaborating on the project?</h5>At UCT Prague, we collaborate closely with the Department of Biotechnology and the Department of Biochemistry and Microbiology. Externally, we work with colleagues from the Institute of Organic Chemistry and Biochemistry of the CAS (IOCB Prague) and the Central European Institute of Technology in Brno (CEITEC). Our research naturally necessitates the integration of diverse fields of expertise; it requires combining virology, cell biology, and biochemistry with advanced imaging and modern approaches to study RNA-protein interactions. This is exactly what makes our project capable of addressing complex questions that a single laboratory cannot tackle on its own.<h5>Are there plans to continue the project? Have you brought on new collaborators, and are you looking to welcome new colleagues to the team?</h5>Yes, definitely. In basic research, it often happens that one answered question opens up several more. High-quality, properly interpreted data provide a foundation you can build on — and that is what moves scientists forward. It is already clear that the topic of retroviral RNA export and packaging has broader implications and may reveal more general principles of how RNA functions within the cell. We also hope the project will be attractive to students, as it connects classical molecular biology, biochemistry, and virology with cutting-edge biotechnological methods. Students are already involved in the project as part of their bachelor’s and master’s theses.<h5>What was the personal turning point that motivated you to pursue a long-term investigation into retroviral particles?</h5>I first encountered retroviruses in the early 1990s during my diploma thesis, when I was working at IOCB Prague on HIV-1 protease inhibitors. At that time, AIDS could not yet be effectively treated, and retrovirus research had a completely different dimension. From the very beginning, I was fascinated by how such a relatively small and simple virus can outsmart evolutionarily highly optimized cellular processes and turn them to its own advantage. And that has never stopped fascinating me. From a scientific point of view, retroviruses are remarkable; just consider one of their enzymes, reverse transcriptase, without which no diagnostic or research laboratory in the world could function today. Without it, it would be impossible to diagnose RNA viruses like SARS-CoV-2, influenza, or HIV using RT-PCR tests, or, for example to produce recombinant human proteins in bacterial cells.

Tracking unspliced RNA: Professor Rumlová studies how retroviruses “hack” cells

High-performance liquid chromatography (HPLC) remains one of the most widely used analytical techniques for separating, identifying, and quantifying compounds in complex mixtures. Successful HPLC method development depends not only on selecting the correct column and optimizing the mobile phase composition, but also on choosing the appropriate detector. Even a perfectly optimized chromatographic separation provides little value if the detection system cannot properly capture and interpret the analytes of interest.In many ways, the detector acts as the “vision system” of the chromatograph. It converts separated compounds into measurable electronic signals, allowing analysts to identify components and determine their concentrations. The choice of detector therefore has a major impact on sensitivity, selectivity, robustness, and the overall quality of analytical data.This article provides an overview of the most common HPLC detection techniques, discusses their strengths and limitations, and outlines the most important criteria to consider when selecting a detector for a specific analytical task.<h3>HPLC Detection Technologies</h3>HPLC detectors are typically divided into two major categories:<ol><li>Specific detectors (solute-property detectors)</li><li>Bulk property detectors</li></ol>Specific detectors respond to a particular chemical or physical property of the analyte. Ideally, their signal is largely unaffected by changes in mobile phase composition. Common examples include UV/Vis detectors, fluorescence detectors, and mass spectrometers.Bulk property detectors, on the other hand, measure differences between the pure mobile phase and the mobile phase containing analytes. These detectors are considered more universal because they respond to properties shared by many compounds. Refractive index detectors are among the best-known examples, alongside conductivity and light-scattering detectors.Both detector categories have important advantages depending on the application, sample type, and analytical goals.<h3>Common Detector Types Used in HPLC</h3>A wide variety of detectors are available for HPLC systems, ranging from highly universal devices to extremely selective and sensitive technologies.Common detector options include:<ul><li>UV/Vis detectors</li><li>Diode array detectors (DAD/PDA)</li><li>Fluorescence detectors</li><li>Refractive index detectors (RID)</li><li>Conductivity detectors</li><li>Evaporative light scattering detectors (ELSD)</li><li>Charged aerosol detectors (CAD)</li><li>Mass spectrometry detectors (MS)</li></ul>Each technology offers a different balance of sensitivity, selectivity, robustness, operational complexity, and cost.<img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/KNAUER_Detector_overview_with_characteristics_Graphic_by_KNAUER_84b0dd30b1.jpg" alt="KNAUER: Detector overview with characteristics. Graphic by KNAUER." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_KNAUER_Detector_overview_with_characteristics_Graphic_by_KNAUER_84b0dd30b1.jpg 245w," sizes="100vw" width="1293" height="596"><h3>Selecting the Right HPLC Detector</h3>There is no universally ideal detector for all HPLC applications. The best choice depends on several factors, including analyte chemistry, matrix complexity, required detection limits, and instrument compatibility.Careful detector selection is essential for obtaining reliable and meaningful analytical results.The following considerations can help guide the selection process.<h4>1. Chemical and Physical Properties of the Analytes</h4>The chemical nature of the compounds being analyzed is one of the most important selection criteria.Questions to consider include:<ul><li>Do the analytes contain chromophores?</li><li>Are the compounds naturally fluorescent?</li><li>What is their polarity?</li><li>What is their molecular weight?</li><li>Are they volatile or non-volatile?</li></ul>Compounds containing UV-active groups are often well suited for UV/Vis detection, while fluorescent molecules can benefit from the high sensitivity of fluorescence detectors. Non-UV-active compounds may require universal detection approaches such as CAD, ELSD, or mass spectrometry.<h4>2. Required Sensitivity</h4>Detector sensitivity determines the minimum concentration that can be reliably detected and quantified.Routine quality control analyses often rely on UV/Vis detectors because they provide sufficient sensitivity for many applications while remaining relatively simple and cost-effective. However, trace-level analysis or highly complex matrices may require more advanced technologies such as:<ul><li>Fluorescence detection</li><li>Charged aerosol detection</li><li>Mass spectrometry</li></ul>These techniques offer significantly lower detection limits and greater analytical specificity.<h4>3. Detector Linearity</h4>Linearity describes how well detector response correlates with analyte concentration across a defined range.A detector with strong linearity ensures accurate quantification and simplifies calibration procedures. Poor linearity can introduce quantification errors, especially when analyzing samples across broad concentration ranges.<h4>4. Dynamic Range</h4>Dynamic range refers to the concentration interval over which the detector can reliably measure analytes.Applications involving samples with highly variable concentrations require detectors capable of maintaining accurate response over a broad range. A wide dynamic range reduces the need for repeated dilutions or multiple analytical runs.<h4>5. Selectivity</h4>Selectivity defines the detector’s ability to distinguish target analytes from other matrix components.This factor becomes particularly important for complex samples containing many interfering substances. Highly selective detectors reduce the risk of overlapping signals and false positives.Mass spectrometry is often advantageous when dealing with unknown compounds or highly complicated sample matrices because it provides structural information in addition to quantitative data.<h4>6. Budget Considerations</h4>Detector cost is another practical factor that laboratories must evaluate.UV/Vis and refractive index detectors are generally among the more economical choices, making them attractive for routine analyses and standard applications.More sophisticated technologies such as fluorescence detection, CAD, or mass spectrometry typically require:<ul><li>Higher initial investment</li><li>Additional maintenance</li><li>More complex infrastructure</li><li>Greater operational expertise</li></ul>However, the overall value of the detector should always be considered in relation to analytical performance requirements and long-term application needs.<h4>7. Instrument and Mobile Phase Compatibility</h4>The selected detector must be compatible with the operational conditions of the HPLC system.Important parameters include:<ul><li>Flow rate range</li><li>Pressure limitations</li><li>Mobile phase composition</li><li>Solvent compatibility</li></ul>Some detectors are highly sensitive to mobile phase composition or gradient changes, while others tolerate a broader range of operating conditions.Ensuring proper compatibility helps prevent baseline instability, signal interference, and reduced detector performance.<h4>8. Destructive vs. Non-Destructive Detection</h4>Another important consideration is whether the analytical workflow involves only detection or also preparative purification.Non-destructive detectors allow collected fractions to remain intact after detection, which is highly beneficial for preparative chromatography and purification workflows.Destructive detectors, by contrast, consume or alter the sample during measurement and are therefore more suitable for analytical applications where sample recovery is not required.<h4>9. Reliability and Ease of Operation</h4>Practical usability is often just as important as analytical performance.Some detectors are designed for straightforward plug-and-play operation, while others require:<ul><li>Frequent calibration</li><li>Specialized maintenance</li><li>Dedicated gas supplies</li><li>Vacuum systems</li><li>Advanced operator expertise</li></ul>Laboratories should evaluate the available technical support, user experience, and maintenance capabilities before implementing more sophisticated detection technologies.Reliable operation and manageable maintenance requirements can significantly improve laboratory productivity and reduce downtime.Choosing the correct HPLC detector starts with understanding both the sample and the analytical objective. Rather than selecting a detector simply because it is available, laboratories should ensure that the technology aligns with the chemistry of the analytes, required performance characteristics, and workflow demands.In many cases, combining multiple detectors within a single HPLC system can provide even greater analytical insight by delivering complementary information from the same chromatographic separation.<img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/KNAUER_Flow_scheme_for_detector_selection_Graphic_by_KNAUER_486004045f.jpg" alt="KNAUER: Flow scheme for detector selection. Graphic by KNAUER." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_KNAUER_Flow_scheme_for_detector_selection_Graphic_by_KNAUER_486004045f.jpg 245w," sizes="100vw" width="1316" height="808">Interested in learning more? Future articles will explore individual HPLC detection techniques in greater detail, including their operating principles, application areas, and practical advantages for different analytical workflows.

A comprehensive guide for selecting the appropriate HPLC detector

News from LabRulezLCMS Library - Week 20, 2026

Article

Product

Application

Scientists

Science and research

The University of Chemistry and Technology, Prague is a natural centre of first-rate study and research in the area of chemistry in Czechia and is one of the country's largest educational and research institutions focused on technical chemistry, chemical and biochemical technologies, material and chemical engineering, food chemistry, and environmental studies.

UCT Prague

Everything from the world of analytical chemistry in one place. We connect people in solving their problems. At Labrulez you will find all the necessary information easily, quickly and clearly. Stop searching and start finding.

LabRulez

Based in Berlin, KNAUER is a medium-sized, owner-managed company that has been serving the sciences since 1962. We develop and manufacture scientific instruments of superior quality for liquid chromatography, including: Analytical HPLC/UHPLC, Preparative HPLC, Fast protein liquid chromatography (FPLC), Multi-column chromatography/Simulated moving bed (SMB) chromatography. High pressure dosing, in-line detection systems, and osmometry are additional business areas.

KNAUER

Separation Science

Metrohm

The Analytical Scientist

Karl Fischer Titration is a standard analytical method that is applied in many laboratories around the world for precise and fast water determination. However, its precision strongly depends on the correct procedure – in this webinar you will learn the most important steps to consider:
1) Basics of Karl Fischer Titration
2) What is the most suitable Karl Fischer titration method for my sample or sample matrix?
3) Correct titer determination and why is it important?
4) Application development
5) Troubleshooting
This webinar is intended for everybody who is interested in learning more details about Karl Fischer Titration, developing Karl Fischer applications, or working in QC/QA departments, especially lab technicians, lab managers and quality assurance managers.
<blockquote>
Presenter: Michael Margreth (Application Specialist, Metrohm)
</blockquote>
<blockquote>
Michael Margreth studied chemistry at the University of Applied Science in Muttenz, Switzerland. He joined Metrohm in 2005 as an application specialist and gained titration experience in the application laboratory.
</blockquote>
<blockquote>
Since 2008 he mainly focuses on Karl Fischer titration and the determination of water.
</blockquote>
<blockquote>
Presenter: Bettina Jubb Straub (Global Product Manager, Merck KGaA)
</blockquote>
<blockquote>
Bettina is the global product manager and lead expert for titration at Merck KGaA based in Darmstadt, Germany.
</blockquote>
<blockquote>
She has more than 20 years’ experience in the instrumental analysis business, especially with Karl Fischer Titration and general Titration, Bettina is a graduated engineer and holds a degree in Chemical Technology from the University of Applied Sciences, Darmstadt Germany and a degree as Medical Technical Laboratory Assistant from the Medical Technical Academy Esslingen, Germany.
</blockquote>

The Analytical Scientist: Karl Fischer Titration – A step-by-step guide to correct results

Karl Fischer Titration – A step-by-step guide to correct results

Karl Fischer Titration precision strongly depends on the correct procedure – in this webinar you will learn the most important steps to consider.

Karl Fischer Titration - A step-by-step guide

Having comprehensive documentation of chemical analyses is crucial for any pharma QC laboratory. Capturing the entire analytical process – from sample preparation to crucial system components such as sensors and even the chemicals used for the analysis – requires the right data management solution. These solutions should also provide all the features needed for secure and efficient data management in networked processes within large, integrated organizations.
In this upcoming webinar, we will discuss the latest advances in data management solutions for the pharmaceutical QC laboratory.
<h5>Register now and join the discussion to learn:</h5>
<ul>
<li>How digitalization facilitates the integration of all relevant data – for seamless compliance and superior data integrity according to the ALCOA+ principles</li>
<li>How to create secure workflows and manage them efficiently within your organization</li>
<li>About the power of the One Click Audit Trail, integration of smart chemicals, and the next level of compliance and data integrity</li>
</ul>
<blockquote>
Presenter: Simon Böhm, PhD (Product Manager Software, Metrohm International Headquarters)
</blockquote>
<blockquote>
Simon Böhm is Product Manager of the OMNIS Software platform at the Competence Centre Software/Workflow/Platforms at Metrohm International Headquarters in Herisau, Switzerland. Simon started working for Metrohm in 2017, when he joined Metrohm Germany. In 2020 he moved on to join Metrohm International Headquarters. Simon studied chemistry at the University of Constance and earned his PhD from the ETH Zurich in physical chemistry.
</blockquote>

Metrohm: Facing Pharma Lab Audits with Confidence

Facing Pharma Lab Audits with Confidence

In this upcoming webinar, we will discuss the latest advances in data management solutions for the pharmaceutical QC laboratory.

Karl Fischer (KF) titration is an industry standard method for measuring water content in all types of substances. However, for various reasons some substances are not suitable for a direct Karl Fischer titration:
<ul>
<li>They release their water only slowly</li>
<li>The water is released at high temperature only</li>
<li>The substances are poorly soluble in KF solvents</li>
<li>undergo side reactions with KF reagents</li>
<li>They contaminate the titration cell or the electrodes</li>
</ul>
By using the KF oven method, the above-mentioned problems can be avoided. This makes the KF oven method a perfect solution for the analysis of difficult samples.
By viewing this webinar you will learn…
<ul>
<li>how to handle liquid samples and water standards correctly</li>
<li>how to analyze difficult samples</li>
<li>how to properly prepare your titration system with the KF oven</li>
<li>how to prepare your sample</li>
<li>how to select the optimal heating temperature</li>
<li>which reagents and which water standards you should use to obtain accurate results</li>
</ul>
After viewing this webinar you will know how to perform accurate titrations using the Karl Fischer oven method. Additionally, the speakers will reveal tips and tricks to help you in the daily routine. The webinar will finish with a live Q&amp;A session.
This webinar will be moderated by Allison Reiter, Offering Management Lead – Honeywell Research Chemicals.
<blockquote>
Presenter: Dr. Roman Neufeld (Senior Research Scientist, Honeywell)
</blockquote>
<blockquote>
Roman joined Honeywell in 2017 as an Application Chemist, providing support to a global customer base on technical queries and application development for Karl Fischer titration. Since 2018, he has expanded his role to include development of new and innovative Hydranal reagents to stay ahead of market needs.
</blockquote>
<blockquote>
Presenter: Michael Margreth (Sr. Product Specialist, Karl Fischer Titration, Metrohm)
</blockquote>
<blockquote>
Michael Margreth studied chemistry at the University of Applied Science in Muttenz, Switzerland. He joined Metrohm in 2005 as an application specialist and gained titration experience in the application laboratory. Since 2008 he mainly focuses on Karl Fischer titration and the determination of water.
</blockquote>

Metrohm: Tips and tricks for Karl Fischer water determination using the oven method

Tips and tricks for Karl Fischer water determination using the oven method

After viewing this webinar you will know how to perform accurate titrations using the Karl Fischer oven method. 

Tips and tricks for Karl Fischer water determination

Titration, as we know it, is almost 200 years old, but has only gained in importance since. To this day, it is a critical technique used to ensure the quality and safety of many products we consume daily. While not a complicated technique, there are some subtleties that can help ensure your titration results are accurate. Simple electrode care and optimization of basic titration parameters can take the guesswork out of your analysis and help you obtain results you can trust.
During this webinar, Eduardo Simões, Product Specialist for Titration at Metrohm USA, will share best practices for accurate titrations and tips that he’s picked up in his 20 years of performing titrations. Dr. Kerri-Ann Blake, Product Manager for Titration at Metrohm USA, will discuss how updated titration software can improve traceability and alert you to results that do not meet quality standards. The live event will conclude with a Q&amp;A, where you’ll get the chance to ask about your specific titrations. 
<blockquote>
Presenter: Eduardo Simões (Titration Product Specialist, Metrohm USA)
</blockquote>
<blockquote>
Eduardo Simões, MSc is the Titration Product Specialist at Metrohm USA in Riverview, Florida. He received his bachelor and masters in analytical chemistry at the University of Campinas, Brazil. He joined Metrohm Brazil as an application specialist in 2003. Over the last 20 years with Metrohm, he specialized in potentiometry and electroanalysis, gaining extensive field experience in applications and instrument installations, technical support and training. Before moving to the US, Eduardo managed the application lab at Metrohm Brazil where he extended his knowledge to other analytical techniques. In addition, Eduardo has experience developing and implementing ISO methods.
</blockquote>
<blockquote>
Presenter: Kerri-Ann Blake (Titration Product Manager, Metrohm USA)
</blockquote>
<blockquote>
Dr. Kerri-Ann Blake is the Titration Product Manager at Metrohm USA in Riverview, Florida. She earned her PhD in Materials Science and Engineering at the University of Florida where she was a researcher at the Particle Engineering Research Center. In her years with Metrohm USA, she has performed proof of concept studies, internal and external trainings and provided applications support. Kerri-Ann plays a key role in product support and product marketing and also has experience developing and implementing ASTM methods.
</blockquote>

Metrohm: Don’t Miss the Endpoint – Titrate It Right the First Time!

Don’t Miss the Endpoint – Titrate It Right the First Time!

We will share best practices for accurate titrations and tips that he’s picked up in his 20 years of performing titrations.

Three Webinars and a Roundtable with Experts from Merck KGaA, Darmstadt, Germany, Metrohm &amp; Mettler Toledo
A Week Dedicated to Karl Fischer Titration!
Karl Fischer titration is still the most used, most accurate, and fastest method to determine water content in all kinds of sample matrices. This titration week covers different aspects that need to be considered when determining the water content in products, raw materials, and other substances. Experts from the life science business of Merck KGaA, Darmstadt, Germany, Metrohm AG, and Mettler Toledo will present and discuss different steps of the Karl Fischer titration process and how to improve it. Learnings include:
<ul>
<li>Karl Fischer titration process and tips for how to improve it</li>
<li>Karl Fischer oven method and its advantages</li>
<li>Reference materials to use for titer determination, instrument check, and result verification</li>
<li>Parameters needed to validate a Karl Fischer titration application</li>
<li>News and future aspects of Karl Fischer titration</li>
</ul>
<h3>How to Achieve Accurate Water Content Results with Karl Fischer Titration</h3>
Transparent and accurate analysis results are an important prerequisite for meeting your customers&#39; quality expectations on products, raw materials and processes. A central analytical parameter is the water content. Water can influence stability, durability, permeability, or consistency of materials, or the energy consumption, yield, and safety in production processes. Therefore, exact water determination is necessary in a wide variety of industries. The Karl Fischer titration method is still the most used, fastest, and most precise method for measuring water content to get accurate, transparent, and comparable results. By attending this presentation, you will learn:
<ul>
<li>The difference between Volumetry and Coulometry</li>
<li>How to improve your Karl Fischer titration process</li>
<li>The key quality assurance requirement</li>
<li>How to perform an accurate titer determination</li>
<li>Tips to avoid failures</li>
</ul>
<blockquote>
Presenter: Bettina Straub-Jubb (Product manager &amp; Lead Expert for Titration at the life science business of Merck KGaA, Darmstadt, Germany)
</blockquote>

Separation Science: How to Achieve Accurate Water Content Results with Karl Fischer Titration

How to Achieve Accurate Water Content Results with Karl Fischer Titration

Topics: The difference between Volumetry and Coulometry, How to improve your Karl Fischer titration process, The key quality assurance requirement ...

Accurate Water Content Results with Karl Fischer Titration

Three Webinars and a Roundtable with Experts from Merck KGaA, Darmstadt, Germany, Metrohm &amp; Mettler Toledo
A Week Dedicated to Karl Fischer Titration!
Karl Fischer titration is still the most used, most accurate, and fastest method to determine water content in all kinds of sample matrices. This titration week covers different aspects that need to be considered when determining the water content in products, raw materials, and other substances. Experts from the life science business of Merck KGaA, Darmstadt, Germany, Metrohm AG, and Mettler Toledo will present and discuss different steps of the Karl Fischer titration process and how to improve it. Learnings include:
<ul>
<li>Karl Fischer titration process and tips for how to improve it</li>
<li>Karl Fischer oven method and its advantages</li>
<li>Reference materials to use for titer determination, instrument check, and result verification</li>
<li>Parameters needed to validate a Karl Fischer titration application</li>
<li>News and future aspects of Karl Fischer titration</li>
</ul>
<h3>The Karl Fischer Oven Method – Step by Step</h3>
arl Fischer (KF) titration is a standard method for the water content determination in all types of substances. However, for various reasons, some substances are not suitable for a direct Karl Fischer titration. These reasons include that they undergo side reaction with KF reagents; they contaminate the titration cell and/or the electrodes; the substances are poorly soluble in alcohol; they release their water only slowly; or the water is released at high temperatures only. These problems can be avoided by using the oven method. This makes the KF oven method the perfect solution for the analysis of difficult samples. By attending this presentation, you will learn:
<ul>
<li>The principle of the oven method</li>
<li>How to properly prepare your titration system with an oven</li>
<li>How to prepare your sample</li>
<li>How to select the optimal oven temperature</li>
</ul>
After viewing this webinar, you will know how to perform accurate sample measurements using the oven method.
<blockquote>
Presenter: Michael Margreth (Application Specialist - Karl Fischer Titration and Water Determination, Metrohm)
</blockquote>

The Karl Fischer Oven Method – Step by Step

After viewing this webinar, you will know how to perform accurate sample measurements using the oven method.

Three Webinars and a Roundtable with Experts from Merck KGaA, Darmstadt, Germany, Metrohm &amp; Mettler Toledo
A Week Dedicated to Karl Fischer Titration!
Karl Fischer titration is still the most used, most accurate, and fastest method to determine water content in all kinds of sample matrices. This titration week covers different aspects that need to be considered when determining the water content in products, raw materials, and other substances. Experts from the life science business of Merck KGaA, Darmstadt, Germany, Metrohm AG, and Mettler Toledo will present and discuss different steps of the Karl Fischer titration process and how to improve it. Learnings include:
<ul>
<li>Karl Fischer titration process and tips for how to improve it</li>
<li>Karl Fischer oven method and its advantages</li>
<li>Reference materials to use for titer determination, instrument check, and result verification</li>
<li>Parameters needed to validate a Karl Fischer titration application</li>
<li>News and future aspects of Karl Fischer titration</li>
</ul>
<h3>Importance of CRM-grade Karl Fischer Standards for Accurate KF Titration</h3>
Karl Fischer (KF) titration is one of the most rapid and accurate methods to determine water content in various samples. For accurate KF titration, high quality Certified Reference Materials (CRMs) are essential. In this webinar, we will present key reference material terms, different quality grades of reference materials, and an overview of our portfolio of high-quality CRM-grade KF Standards. By attending this presentation, you will learn:
<ul>
<li>Key terms: Metrological Traceability, Quality Grades, 17025 &amp; 17034 Accreditation</li>
<li>The importance of using the right quality grade of KF standards for your application</li>
<li>An overview of our unmatched portfolio of KF standards</li>
</ul>
<blockquote>
Presenter: Shailly Krishna-Rajusth (Product Manager for Physical &amp; Chemical Property Standards at the life science business of Merck KGaA, Darmstadt, Germany)
</blockquote>

Separation Science: Importance of CRM-grade Karl Fischer Standards for Accurate KF Titration

Importance of CRM-grade Karl Fischer Standards for Accurate KF Titration

Key reference material terms, different quality grades of reference materials, and an overview of our portfolio of high-quality CRM-grade KF Standards.

CRM-grade Karl Fischer Standards for Accurate KF Titration

Three Webinars and a Roundtable with Experts from Merck KGaA, Darmstadt, Germany, Metrohm &amp; Mettler Toledo
A Week Dedicated to Karl Fischer Titration!
Karl Fischer titration is still the most used, most accurate, and fastest method to determine water content in all kinds of sample matrices. This titration week covers different aspects that need to be considered when determining the water content in products, raw materials, and other substances. Experts from the life science business of Merck KGaA, Darmstadt, Germany, Metrohm AG, and Mettler Toledo will present and discuss different steps of the Karl Fischer titration process and how to improve it. Learnings include:
<ul>
<li>Karl Fischer titration process and tips for how to improve it</li>
<li>Karl Fischer oven method and its advantages</li>
<li>Reference materials to use for titer determination, instrument check, and result verification</li>
<li>Parameters needed to validate a Karl Fischer titration application</li>
<li>News and future aspects of Karl Fischer titration</li>
</ul>
<h3>Direct Karl-Fischer Titration or Gas Phase Extraction – A Closer Look</h3>
In this presentation we will compare the direct addition with the gas phase extraction method. For this purpose, various sample types were analyzed by either direct addition, gas phase extraction, or both. Among the tested sample types are powders, oils, and cannabis flower. We will discuss the challenges that such samples represent and highlight the analytical measures that will ensure to obtain the best results possible. By attending this presentation, you will learn about:
<ul>
<li>Direct titration vs. gas phase extraction method</li>
<li>Advantages of the Karl Fischer oven method (gas phase extraction)</li>
<li>Application examples: oils / cannabis flower</li>
</ul>
<blockquote>
Presenter: Thomas Hitz, PhD (Application Development, Automated Karl-Fischer Titration, Mettler Toledo)
</blockquote>

Separation Science: Direct Karl-Fischer Titration or Gas Phase Extraction – A Closer Look

Direct Karl-Fischer Titration or Gas Phase Extraction – A Closer Look

In this presentation we will compare the direct addition with the gas phase extraction method.

Direct Karl-Fischer Titration or Gas Phase Extraction

Three Webinars and a Roundtable with Experts from Merck KGaA, Darmstadt, Germany, Metrohm &amp; Mettler Toledo
A Week Dedicated to Karl Fischer Titration!
Karl Fischer titration is still the most used, most accurate, and fastest method to determine water content in all kinds of sample matrices. This titration week covers different aspects that need to be considered when determining the water content in products, raw materials, and other substances. Experts from the life science business of Merck KGaA, Darmstadt, Germany, Metrohm AG, and Mettler Toledo will present and discuss different steps of the Karl Fischer titration process and how to improve it. Learnings include:
<ul>
<li>Karl Fischer titration process and tips for how to improve it</li>
<li>Karl Fischer oven method and its advantages</li>
<li>Reference materials to use for titer determination, instrument check, and result verification</li>
<li>Parameters needed to validate a Karl Fischer titration application</li>
<li>News and future aspects of Karl Fischer titration</li>
</ul>
<h3>Validation of Parameters for Karl Fischer Titration</h3>
Validation of parameters for a Karl Fischer titration method is an essential prerequisite for laboratories. Performance characteristics of the procedure need to meet the requirements for its intended purpose. You will learn typical analytical performance parameters that should be considered in the validation of the Karl Fischer titration procedures. The objective of this session is to present the requirements for a titration process.
By attending this presentation, you will learn about:
<ul>
<li>General aspects of validation</li>
<li>Required validation parameters</li>
<li>How to verify the suitability and accuracy of a titration method</li>
<li>An overview of the parameters described in ICH (International Council for Harmonization) guidance Q2(R1) and USP general chapter &lt;1225&gt; (selectivity, linearity, accuracy &amp; reproducibility)</li>
</ul>
<blockquote>
Presenter: Angela Schwehn (Laboratory Scientist in the R&amp;D Department of the life science business of Merck KGaA, Darmstadt, Germany)
</blockquote>

Separation Science: Validation of Parameters for Karl Fischer Titration

Validation of Parameters for Karl Fischer Titration

The objective of this session is to present the requirements for a titration process.

Three Webinars and a Roundtable with Experts from Merck KGaA, Darmstadt, Germany, Metrohm &amp; Mettler Toledo
A Week Dedicated to Karl Fischer Titration!
Karl Fischer titration is still the most used, most accurate, and fastest method to determine water content in all kinds of sample matrices. This titration week covers different aspects that need to be considered when determining the water content in products, raw materials, and other substances. Experts from the life science business of Merck KGaA, Darmstadt, Germany, Metrohm AG, and Mettler Toledo will present and discuss different steps of the Karl Fischer titration process and how to improve it. Learnings include:
<ul>
<li>Karl Fischer titration process and tips for how to improve it</li>
<li>Karl Fischer oven method and its advantages</li>
<li>Reference materials to use for titer determination, instrument check, and result verification</li>
<li>Parameters needed to validate a Karl Fischer titration application</li>
<li>News and future aspects of Karl Fischer titration</li>
</ul>
<h3>Live Roundtable Expert Discussion (Karl Fisher Titration Week)</h3>
Together with our partners Metrohm and Mettler Toledo, Merck KGaA, Darmstadt, Germany will discuss the trends and key aspects of Karl Fischer titration. We will come back on some questions asked during the earlier three webinars, as well as answering new questions during the roundtable discussion. 
This live event will be moderated by Separation Science and participants will include Bettina Straub-Jubb, Dr. Shailly Krishna Rajusth, and Angela Schwehn from Merck Life Science KGaA, Darmstadt, Germany, Dr. Leila Mosberger from Mettler-Toledo, and Michael Margreth from Metrohm AG.

LCMS webinars focusing on Titration - page 4

Live Roundtable Expert Discussion (Karl Fisher Titration Week)