Scientists at IOCB Prague are uncovering new details of gene transcription. They have identified a previously unknown molecular mechanism by which the transcription of genetic information from deoxyribonucleic acid (DNA) into ribonucleic acid (RNA) can be initiated. The researchers focused on a specific class of molecules known as alarmones, which are found in cells across a wide range of organisms and whose levels often increase under conditions of cellular stress. The results were published in the prestigious scientific journal Nature Chemical Biology.<img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/UOCHB_Vyzkum_z_UOCHB_odhaluje_dosud_nepoznany_zpusob_prepisu_geneticke_informace_bc2bfffa6c.jpg" alt="IOCB Prague/Tomáš Belloň: Research from IOCB Prague reveals a previously unknown mechanism of genetic transcription: Dr. Hana Cahová, head of the Chemical Biology of Nucleic Acids research group." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_UOCHB_Vyzkum_z_UOCHB_odhaluje_dosud_nepoznany_zpusob_prepisu_geneticke_informace_bc2bfffa6c.jpg 234w," sizes="100vw" width="2000" height="1335"><div>Molecules of ribonucleic acid can carry a variety of chemical modifications at one end, known as caps. In eukaryotic organisms, including human cells, the best-known cap plays an important role in RNA stability and in regulating its subsequent fate. In recent years, however, it has become clear that alternative, non-canonical RNA caps also exist, although their formation and the mechanisms by which they are attached to RNA remain only partially understood. These include alarmone caps, formed by dinucleoside polyphosphate molecules, that protect cellular RNA at moments when the cell is under threat.In their study, <a target="_blank" rel="noopener noreferrer" href="https://cahova.group.uochb.cz/en">Dr. Hana Cahová</a> and colleagues examined bacterial RNA polymerase and investigated how this enzyme can initiate transcription using dinucleoside polyphosphates (NpNs) instead of the standard RNA building blocks. For the first time, the scientists described, at the atomic level, how RNA bearing an alarmone cap can be generated directly at the initiation of gene transcription. They also observed that NpNs bind through a different type of base pairing than is typically observed.The work was significantly shaped by Valentina Serianni from Hana Cahová’s team, who demonstrated the ability of dinucleoside polyphosphates to initiate gene transcription, and by Jana Škerlová, who focused on the structural analysis of RNA polymerase. Using cryogenic electron microscopy data, she showed how dinucleoside polyphosphate molecules bind within the active site of RNA polymerase – that is, the core of the enzyme where genetic information is transcribed. Together, their findings help to elucidate the processes that accompany the transcription of genetic information from DNA to RNA.Hana Cahová adds: “We’re describing something that truly occurs in cells and that we’re now able to observe directly at the level of individual molecules. This allows us to answer fundamental questions about cellular processes, such as how cells adapt to stress. RNA plays a central role in this, as it carries the cascade of information underlying any cellular response – for example, to threatening conditions caused by nutrient deprivation or temperature shock.”<img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/UOCHB_Vyzkum_z_UOCHB_odhaluje_dosud_nepoznany_zpusob_prepisu_geneticke_informace_2_0fe88ff9e9.jpg" alt="IOCB Prague/Tomáš Belloň: Research from IOCB Prague reveals a previously unknown mechanism of genetic transcription: Dr. Tomáš Kouba, head of the Cryogenic Electron Microscopy core facility." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_UOCHB_Vyzkum_z_UOCHB_odhaluje_dosud_nepoznany_zpusob_prepisu_geneticke_informace_2_0fe88ff9e9.jpg 234w," sizes="100vw" width="2000" height="1335"><div>Cryogenic electron microscopy (cryo-EM) was central to the project. This part of the work was led by Dr. Tomáš Kouba, one of the authors of the study. “Cryogenic electron microscopy allows us to freeze biological molecules in a state very close to their natural form and then determine their three-dimensional structure. This makes it possible to look directly into the active centers of enzymes and observe their function down to the atomic level,” he explains.Last year, IOCB Prague opened a new <a target="_blank" rel="noopener noreferrer" href="https://cryoem.group.uochb.cz/en">cryo-EM center</a> that is unique within the Czech research environment. State-of-the-art cryogenic electron microscopes are housed in a specially designed building and enable the study of biological processes with exceptional structural precision.<img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/UOCHB_Vyzkum_z_UOCHB_odhaluje_dosud_nepoznany_zpusob_prepisu_geneticke_informace_3_4d881e7a58.jpg" alt="IOCB Prague: Research from IOCB Prague reveals a previously unknown mechanism of genetic transcription: This work shows how NpnNs initiate bacterial transcription. Cryo-EM reveals how nucleobases pair with the template: one in canonical and one in reverse Watson–Crick mode, providing a structural basis for their function as non-canonical RNA caps." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_UOCHB_Vyzkum_z_UOCHB_odhaluje_dosud_nepoznany_zpusob_prepisu_geneticke_informace_3_4d881e7a58.jpg 245w," sizes="100vw" width="2000" height="1077"><blockquote><h4>The Original article</h4><h4><a target="_blank" rel="noopener noreferrer" href="https://www.nature.com/articles/s41589-025-02134-5">Molecular insight into 5′ RNA capping with NpnNs by bacterial RNA polymerase</a></h4>Valentina M. Serianni, Jana Škerlová, Anna Knopp Dubánková, Anton Škríba, Hana Šváchová, Tereza Vučková, Anatolij Filimoněnko, Milan Fábry, Pavlína Řezáčová, Tomáš Kouba &amp; Hana Cahova&nbsp;Nat. Chem. Biol. 21 (2026)<a target="_blank" rel="noopener noreferrer" href="https://doi.org/10.1038/s41589-025-02134-5">https://doi.org/10.1038/s41589-025-02134-5</a><h6>licensed under <a target="_blank" rel="noopener noreferrer" href="https://creativecommons.org/licenses/by/4.0/">CC-BY 4.0</a></h6></blockquote><h4>Abstract</h4>RNA capped with dinucleoside polyphosphates has been discovered in bacteria and eukaryotes only recently. The likely mechanism of this specific capping involves direct incorporation of dinucleoside polyphosphates by RNA polymerase as noncanonical initiating nucleotides. However, how these compounds bind into the active site of RNA polymerase during transcription initiation is unknown. Here, we explored transcription initiation in vitro, using a series of DNA templates in combination with dinucleoside polyphosphates and model RNA polymerase from Thermus thermophilus. We observed that the transcription start site can vary on the basis of the compatibility of the specific template and dinucleoside polyphosphate. Cryo-electron microscopy structures of transcription initiation complexes with dinucleoside polyphosphates revealed that both nucleobase moieties can pair with the DNA template. The first encoded nucleotide pairs in a canonical Watson–Crick manner, whereas the second nucleobase pairs noncanonically in a reverse Watson–Crick manner. Our work provides a structural explanation of how dinucleoside polyphosphates initiate RNA transcription.</div></div>

Research from IOCB Prague reveals a previously unknown mechanism of genetic transcription

Food adulteration is a phenomenon with a long history that has gained increasing importance in recent years due to the globalization of food supply chains and strong economic pressures. Whether it involves diluting olive oil with cheaper oils, adding sugar to honey, or the hidden use of substitutes in protein supplements, the result is a loss of consumer trust and, in some cases, a potential risk to health. Analytical chemistry plays a key role in this area by providing methods and technologies capable of identifying and quantifying target compounds as well as unwanted impurities in a wide range of food matrices.<img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Watrex_Praha_Falsovani_potravin_a_jak_ho_odhali_analyticka_chemie_6912db5492.jpg" alt="Watrex: Food Adulteration and How It Is Revealed by Analytical Chemistry" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Watrex_Praha_Falsovani_potravin_a_jak_ho_odhali_analyticka_chemie_6912db5492.jpg 245w," sizes="100vw" width="1600" height="900"><h4>The Role of Chromatography in Forensic Food Analysis</h4>Chromatography, particularly liquid chromatography (HPLC), is one of the most effective analytical tools for separating complex mixtures of molecules. It enables:<ul><li>qualitative identification of individual components in a sample,</li><li>quantitative determination of their concentrations,</li><li>detection of impurities and unexpected components that may indicate adulteration.</li></ul>In practice, HPLC is often combined with various types of detectors (UV/VIS, DAD, refractive index detectors) or with other techniques (e.g., mass spectrometry) to increase selectivity and sensitivity.<h4>Isocratic vs. Gradient Elution</h4>In the analysis of food samples, chromatographic methods are selected according to the analytical goal:<ul><li>Isocratic HPLC uses a constant mobile phase composition throughout the analysis. It is simple, robust, and ideal for routine determinations of well-defined target analytes (e.g., sugars or vitamins).</li><li>Gradient HPLC changes the mobile phase composition during separation, enabling better resolution of complex mixtures with a wide range of polarities (e.g., peptides or phenolic compounds).</li></ul>Gradient methods are particularly useful when analytes have very different properties and high separation efficiency is required.<h4>Watrex Praha and the Contribution of Modern Systems to Food Analysis</h4><a target="_blank" rel="noopener noreferrer" href="https://watrex.com/?utm_source=labrulez&amp;utm_medium=propagace&amp;utm_campaign=labrulez_clanky_HPLCsystemy">Watrex Praha, s.r.o.</a> is one of the leading Czech manufacturers and distributors of liquid chromatography systems and instruments, with a long tradition dating back to 1990. The company has successfully transformed from a distributor into a manufacturer of its own HPLC and related technologies, focusing on modular, robust systems suitable even for demanding analytical tasks.Their <a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/products?manufacturerItems=93&amp;search=streamline">Streamline™ product line</a> includes several HPLC system concepts suitable for food analysis:<ul><li>Isocratic HPLC system – a simple yet precise configuration with a single pump, column thermostat, and UV/VIS detector; suitable for routine determination of known analytes (e.g., basic carbohydrates, food additives).</li><li>Binary gradient HPLC system – equipped with two pumps enabling mobile phase gradient formation, which enhances separation performance for complex mixtures (e.g., phenolic profiles of wine, components of aromatic extracts).</li><li>GPC/SEC system (Gel Permeation / Size Exclusion Chromatography) – a specialized solution for the determination of macromolecules and polymers, also relevant for the analysis of high-molecular-weight components in foods (e.g., polysaccharides).</li></ul><blockquote>👉<a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/labrulez-bucket-strapi-h3hsga3/Watrex_catalog_2024_ba97395f92.pdf">Product catalogue HERE</a></blockquote>Thanks to the modular nature of these systems, analysts can easily adapt the configuration to a specific analytical problem, from simple routine analyses to complex characterization of food samples.<h4>Practical Examples of Adulteration Revealed by HPLC</h4><ul><li>Detection of olive oil dilution: HPLC can separate and quantify characteristic triacylglycerols and fatty acids; unusual patterns indicate the presence of cheaper oils.</li><li>Estimation of sugar addition in honey: Isocratic HPLC with a refractive index detector can determine sugar profiles (glucose, fructose, sucrose), revealing dilution or the addition of sugar syrups.</li><li>Identification of aromatic adulterants in wine: Gradient HPLC with a PDA detector can reveal phenolic and aromatic profiles that are altered by the addition of synthetic compounds.</li></ul><h4>Conclusion</h4>Food adulteration remains a current issue that requires reliable and sensitive analytical methods. Liquid chromatography, and in particular modern HPLC systems, represent one of the most powerful tools for detecting unwanted additives, impurities, and unexpected components in complex food samples. Manufacturers such as <a target="_blank" rel="noopener noreferrer" href="https://watrex.com/?utm_source=labrulez&amp;utm_medium=propagace&amp;utm_campaign=labrulez_clanky_HPLCsystemy">Watrex Praha</a> provide flexible chromatographic platforms covering a wide range of analytical tasks from simple isocratic to advanced gradient and GPC/SEC applications thereby supporting analysts in ensuring food quality and consumer protection.<blockquote>Did you find this article interesting? Follow <a target="_blank" rel="noopener noreferrer" href="https://watrex.com/?utm_source=labrulez&amp;utm_medium=propagace&amp;utm_campaign=labrulez_clanky_HPLCsystemy">Watrex Praha</a> on their social media (<a target="_blank" rel="noopener noreferrer" href="https://www.linkedin.com/company/watrex/?viewAsMember=true">LinkedIn</a> and <a target="_blank" rel="noopener noreferrer" href="https://www.facebook.com/WatrexPrague">Facebook</a>) for more engaging topics.</blockquote>

Food Adulteration and How It Is Revealed by Analytical Chemistry

Join us for a fascinating conversation with Dr. Subhoja Chakraborty from the University of Central Florida, whose cutting-edge research targets the global challenge of malaria drug resistance. Dr. Chakraborty shares her journey from structure-based drug design during her PhD to her recent collaborative work repurposing human kinase inhibitors as rapid, selective antimalarials against drug-resistant Plasmodium falciparum strains.<h4>In this episode, you'll learn:</h4><ul><li>How structure-guided approaches reveal potential drug targets, including cysteine proteases like falcipain-2, and kinases implicated in malaria's complex life cycle.</li><li>The power of repurposing kinase inhibitors originally developed for cancer and other diseases, focusing on compound 12, which blocks hemozoin formation and protein kinase 6—key steps in parasite survival.</li><li>Lab techniques such as affinity, gel filtration, and size exclusion chromatography for recombinant protein purification—and how troubleshooting is crucial for high-yield, stable complexes.</li><li>Advances in mass spectrometry for characterizing protein interactions, validating drug targets, and mapping molecular pathways pivotal to malaria therapeutics.</li><li>Tips and lessons for new researchers facing the challenges of sample preparation, complex protocols, and working as part of a collaborative team.</li></ul>Dr. Chakraborty’s insights bridge the gap between basic science and translational drug discovery, offering hope for more effective, targeted malaria treatments in a world where resistance to frontline therapies threatens millions.<h4>Referenced Papers:</h4><ul><li>"Plasmodium falciparum protein kinase 6 and hemozoin formation are inhibited by a type II human kinase inhibitor exhibiting antimalarial activity" (Cell Chemical Biology, 2025)</li><li>"New insights of falcipain 2 structure from Plasmodium falciparum 3D7 strain" (BBRC, 2022)</li><li>"Structure-Based Optimization of Protease−Inhibitor Interactions to Enhance Specificity of Human Stefin‑A against Falcipain‑2 from the Plasmodium falciparum 3D7 Strain" (Biochemistry, 2023)</li></ul>If you’re a student, scientist, or simply passionate about advances in infectious disease research, this episode offers actionable insights, inspiring advice, and a front-row seat to innovation in combating malaria.<h3>Video Transcription</h3><h4>From Structural Biology to Drug Discovery: Chromatography and Mass Spectrometry in Malaria Research</h4><h5>Introduction</h5>In this discussion, Subhoja shares insights into her research journey, focusing on malaria drug discovery and the role of modern analytical techniques in biological and biomedical research. The conversation is aimed primarily at undergraduate students and early-career researchers interested in chemistry, biotechnology, and life sciences.<h5>Research Focus: Malaria Drug Discovery and Kinase Targets</h5>Subhoja is currently involved in malaria drug discovery research at the University of Central Florida, where she has been working since beginning her postdoctoral position two years ago. Her earlier PhD research focused on structure-based drug design in malaria, using a combination of in silico approaches, recombinant protein expression, and X-ray crystallography to determine precise three-dimensional structures of target proteins and their inhibitors.This structural perspective provided a foundation for her current work, which centers on drug discovery through compound screening and repurposing. A recent publication from her laboratory explores the use of repurposed mammalian CDK-like kinase inhibitors as potential antimalarial agents. While such kinase inhibitors are well known in cancer and cardiovascular research, their application to malaria targets represents a novel and promising approach.Kinases are attractive drug targets because of their central role in cell signaling and growth pathways. By targeting specific kinases involved in different stages of the malaria parasite life cycle—whether asexual growth, gametocytogenesis, or differentiation—it may be possible to significantly inhibit parasite growth and progression.<h5>Chromatography in Protein Expression and Purification Workflows</h5>Chromatography plays a critical role throughout Subhoja’s research. Her experience spans from basic chromatography techniques during her early training to advanced protein purification workflows using affinity chromatography, gel filtration (size exclusion chromatography), and ion exchange chromatography.Recombinant proteins are routinely purified using affinity resins such as nickel or cobalt, followed by further purification to remove contaminants. Fast Protein Liquid Chromatography (FPLC) enables compact, stepwise purification workflows suitable for producing proteins for in vitro assays.A recurring challenge in this process is the expression of high-molecular-weight proteins, which often accumulate in insoluble fractions or inclusion bodies. Careful selection of expression vectors, host systems, and induction conditions is therefore essential to maximize yield and solubility.<h5>Integrating Size Exclusion Chromatography into the Workflow</h5>Size exclusion chromatography (SEC) is typically used after affinity purification to further improve protein purity. Following elution with imidazole or glutathione, desalting is required to prevent buffer components from interfering with downstream assays. This is often achieved using desalting columns such as G-25 or PD-10.To remove lower-molecular-weight contaminants, finer SEC media—such as Superdex or Sephacryl columns—are employed. Concentration steps using molecular-weight cut-off filters are then applied to obtain the desired protein concentration for functional studies.These iterative purification and troubleshooting steps are a routine part of protein biochemistry and reflect the importance of methodical optimization and careful validation.<h5>The Role of Mass Spectrometry in Biomolecular Characterization</h5>Mass spectrometry is a key analytical tool in Subhoja’s research pipeline, particularly for target identification and validation. In both malaria and cancer research, MS is used alongside chromatography, immunoprecipitation, and pull-down assays to study protein interactions and molecular recognition events.Affinity-based mass spectrometry enables the identification of binding partners and interactors, whether studying proteins, long non-coding RNAs, or protein complexes. Techniques such as native MS, tandem affinity purification, and proximity labeling (e.g., TurboID-based workflows) provide deeper insights into molecular networks and signaling pathways.These approaches are essential for understanding how candidate drug targets function within complex biological systems.<h5>Sample Preparation Challenges in Biological Research</h5>Sample preparation remains one of the most challenging aspects of biological experimentation. Whole-cell lysates contain proteases, nucleases, and other components that can rapidly degrade target molecules. Preventing degradation requires minimizing preparation time, maintaining low temperatures, and using appropriate inhibitor cocktails.Reproducibility is also critical: biological experiments require multiple replicates, and variability is often unavoidable. Careful handling, protocol optimization, and continuous troubleshooting are essential to ensure reliable results, whether preparing samples for chromatography, mass spectrometry, sequencing, or immunoprecipitation.<h5>Advances in Chromatography and MS for Malaria Research</h5>Modern advances in chromatography and mass spectrometry significantly enhance malaria research by enabling precise target identification, quantification, and functional characterization. Drug discovery workflows begin with construct design and recombinant expression, followed by purification and interaction studies.In malaria research, stage-specific parasite biology requires careful extraction and analysis of proteins from different life cycle stages. Tandem affinity MS, immunoprecipitation, and in vivo validation studies help confirm target relevance and therapeutic potential.Recent work on repurposed kinase inhibitors has shown promising results, including inhibition of hemozoin formation—a key detoxification pathway for the malaria parasite—highlighting the potential of such compounds for further development.<h5>Malaria, Drug Resistance, and Global Impact</h5>Malaria remains a major global health burden, with over 600,000 deaths annually and widespread drug resistance complicating treatment. Artemisinin resistance, particularly associated with mutations in the K13 propeller domain, has driven the search for new targets and therapies.During her PhD, Subhoja focused on falcipain-2, a cysteine protease involved in hemoglobin degradation within the parasite. This pathway is critical for parasite survival and has emerged as a promising drug target. Her work revealed that falcipain-2 operates within a larger protein complex responsible for hemoglobin processing and amino acid release.Understanding these molecular mechanisms is essential for developing robust, stage-specific antimalarial strategies.<h5>Advice for Early-Career Researchers</h5>For researchers just starting out, Subhoja emphasizes the importance of hands-on experience. Working directly with cells, lysates, and analytical tools is essential for truly understanding experimental systems. Failure is an inherent part of research, and perseverance is key.Motivation, teamwork, careful sample handling, and thorough preparation all contribute to long-term success. Reading extensively, critically evaluating one’s own work, and appreciating incremental progress are vital aspects of a sustainable research career.Every successful experiment—no matter how small—represents meaningful progress and contributes to the broader goals of science and medicine.<h5>Closing Remarks</h5>The discussion highlights how interdisciplinary approaches combining chromatography, mass spectrometry, structural biology, and biochemistry are driving advances in malaria drug discovery. Through careful experimental design, analytical rigor, and collaborative teamwork, researchers can address complex biological challenges and contribute to global health solutions.<blockquote>This text has been automatically transcribed from a video presentation using AI technology. It may contain inaccuracies and is not guaranteed to be 100% correct.</blockquote><blockquote><h4>Concentrating on Chromatography Podcast</h4>Dive into the frontiers of chromatography, mass spectrometry, and sample preparation with host David Oliva. Each episode features candid conversations with leading researchers, industry innovators, and passionate scientists who are shaping the future of analytical chemistry. From decoding PFAS detection challenges to exploring the latest in AI-assisted liquid chromatography, this show uncovers practical workflows, sustainability breakthroughs, and the real-world impact of separation science. Whether you’re a chromatographer, lab professional, or researcher you'll discover inspiring content!You can find Concentrating on Chromatography Podcast in podcast apps:<ul><li><a target="_blank" rel="noopener noreferrer" href="https://open.spotify.com/show/1wZg67rCjoGju4yhqxI3tL">Spotify</a></li><li><a target="_blank" rel="noopener noreferrer" href="https://podcasts.apple.com/tz/podcast/concentrating-on-chromatography/id1788429185">Apple podcast</a></li><li><a target="_blank" rel="noopener noreferrer" href="https://podscan.fm/podcasts/concentrating-on-chromatography">podscan</a></li></ul>and on <a target="_blank" rel="noopener noreferrer" href="https://www.youtube.com/@ChromatographyTalk/videos">YouTube channel</a></blockquote>

Affinity, Gel Filtration, Size Exclusion Chromatography: Repurposing Kinase Inhibitors for Malaria

Article

Product

Video

Scientists

Food

Interview

Science and research

The Institute of Organic Chemistry and Biochemistry of the Czech Academy of Sciences (IOCB Prague) is a leading scientific institution in the Czech Republic, recognized internationally. Its primary mission is basic research in the fields of chemical biology and medicinal chemistry, organic and material oriented chemistry, chemistry of natural compounds, biochemistry and molecular biology, physical chemistry, theoretical chemistry, and analytical chemistry. 

Institute of Organic Chemistry and Biochemistry of the Czech Academy of Sciences

Organomation, founded in 1959, designs and manufactures high-quality nitrogen evaporators and extraction systems. Known for innovation and durability, their lab instruments are used globally for efficient sample preparation with strong customer support.

Organomation

WATREX Prague is the distributor and producer of HPLC techniques in the Czech Republic and Slovakia. Main Chromatografic and Related Techniques Supported: LC, GPC, IC, IEC, Automated SPE, LC Columns and special column packings, LC method development, Software development, Laboratory Robots.

Watrex Praha

Separation Science

Metrohm

Thermo Fisher Scientific

SelectScience

Learn more about KF titration and the oven technique, the choice of a suitable reagent, and the difference between volumetric and coulometric titration.

Metrohm: Karl Fischer titration: Troubleshooting

Karl Fischer titration: Troubleshooting

Learn more about KF titration and the oven technique, the choice of a suitable reagent, and the difference between volumetric and coulometric titration.

For many years/decades, Karl Fischer (KF) titration and Hydranal™ reagents have been the solution of choice for measuring water content in all types of substances.
While modern KF titration is safe and easy to use, there are still some pitfalls when it comes to handling and maintenance that even experienced users may not be aware of.
By viewing this presentation you will learn...
<ul>
<li>what the correct combinations of instrument types and reagents are for KF titration and what will happen if they are not compatible
</li>
<li>how to perform titer determinations and system checks with different types of water standards
</li>
<li>how to calculate sample sizes
</li>
<li>how to select the optimal temperature for heating the sample in a KF oven
</li>
<li>how to protect your system against ambient moisture
</li>
<li>how to properly assemble, disassemble, clean, and store the titration equipment
</li>
</ul>
After viewing this presentation, you will know how to perform accurate Karl Fischer titrations, how to properly use and store your reagents, and how to use and maintain your titration equipment correctly.
<blockquote>
Presenter: Agnieszka Kossakowska (Hydranal Marketing Specialist, Honeywell Research Chemicals)
</blockquote>
<blockquote>
Presenter: Michael Margreth (Senior Product Specialist Titration, Metrohm International Headquarters, Switzerland)
</blockquote>

Separation Science: Everything You Need to Know About Karl Fischer Titration

Everything You Need to Know About Karl Fischer Titration

How to perform accurate Karl Fischer titrations, how to properly use and store your reagents, and how to use and maintain your titration equipment correctly.

This webinar will provide tips and tricks on troubleshooting in titration.

Titration: Troubleshooting

This webinar will provide tips and tricks on troubleshooting in titration.

Watch this webinar to learn about titration methods, titer determination, and sample preparation.

Titration: Fundamentals

Watch this webinar to learn about titration methods, titer determination, and sample preparation.

Titration is one of the most basic analytical methods for chemical analysis.
However, even power users sometimes struggle with the quality of their titrations. In this expert webinar, we explore the basics of volumetric titration and present tips and advice to help you make sure that your titration results are always precise and reproducible. The following topics will be addressed:
What is the most suitable titration method for my sample or sample matrix?
<ul>
<li>Photometric vs. potentiometric titration
</li>
<li>Direct vs. back (residual) titration
</li>
<li>End point vs. equivalent point titration
</li>
</ul>
Hardware selection
<ul>
<li>Sensor/burette size/stirrer</li>
</ul>
Reagent selection
<ul>
<li>Buffer solutions/titrant/standard reference reagents</li>
</ul>
Method development &amp; validation
<ul>
<li>Titration modes &amp; parameters
</li>
<li>Method validation (selectivity, linearity, accuracy &amp; reproducibility)
</li>
</ul>
Key learning objectives
<ul>
<li>How to select the proper hardware and the right chemistry for your titrations
</li>
<li>How to optimize titration performance depending on your application
</li>
<li>How to verify the suitability and accuracy of a titration method
</li>
<li>How to convert manual titration methods into automated procedures
</li>
</ul>
Who should attend?
This webinar will be of particular interest to people in charge of developing or qualifying volumetric titrations, especially lab technicians, lab managers and quality assurance professionals
<blockquote>
Presenter: Dr. Christian Haider (Head of Competence Center Titration, Metrohm AG)
</blockquote>
<blockquote>
Dr. Christian Haider joined Metrohm AG in the year 2002 as Application Specialist Titration. As Head of the Competence Center Titration he leads a team of product managers, product specialists and application chemists for all different titration techniques, pH and conductivity measurement, titration automation and software.
</blockquote>
<blockquote>
Presenter: Angela Schwehn (Chemical Laboratory Technician, Merck KGaA, Darmstadt, Germany)
</blockquote>
<blockquote>
Angela Schwehn is a chemical laboratory technician in the Life Science R&amp;D department at Merck KGaA, Darmstadt, Germany. She has more than 15 years of experience in modern volumetric titration and Karl Fischer titration and is responsible for the development of new reagents and standards in the field of volumetric titration and Karl Fischer titration.
</blockquote>
<blockquote>
Presenter: Sarah Thomas (Editorial Team, SelectScience)
</blockquote>
<blockquote>
Sarah studied biology at the University of Bath, UK, and has worked in the Pharmaceutical Sciences Department of St. Jude Children’s Research Hospital, Memphis. As a member of the Editorial team, Sarah plays an integral role in shaping the content on SelectScience.
</blockquote>
<blockquote>
Presenter: Ellie Abbott (Editorial Team, SelectScience)
</blockquote>
<blockquote>
Ellie studied biochemistry at the University of Nottingham, UK, and has previous experience working in management consultancy. As a member of the Editorial team, she now plays an integral role in shaping the content on SelectScience.
</blockquote>

Metrohm: Volumetric titration: A step-by-step guide to correct results

Volumetric titration: A step-by-step guide to correct results

The basics of volumetric titration and present tips and advice to help you make sure that your titration results are always precise and reproducible.

Volumetric titration: A step-by-step guide to correct result

In most laboratories, the age of manual titrations is over. Good so: Modern, digital autotitrators support users with various features that help reduce errors and optimize titration results.
However, even routine users are sometimes struggling with the basics, e.g., which kind of electrode to choose for which titration, which titration mode to select for which matrix, or how to make sure results are properly evaluated by the device.
In this presentation, we focus on how to get the basics of titration right so you can always trust your results:
<ul>
<li>How to select the right hardware for your titration (electrode, burette size, stirrer)
</li>
<li>How to handle your hardware properly (this is critical!)
</li>
<li>How to choose the correct settings and parameters on your autotitrator, depending on your matrix and method
</li>
<li>How endpoints are evaluated correctly and how to optimize endpoint evaluation
</li>
<li>Troubleshooting: What to do in case you get incorrect results
</li>
</ul>
This presentation is intended for all users of potentiometric titrators, chemists developing titration applications, laboratory managers – simply everyone who is interested in modern titration.
<blockquote>
Presenter: ChristianHaiderWebDr. Christian Haider (Head of Competence Center Titration, Metrohm AG)
</blockquote>
<blockquote>
Dr. Haider joined Metrohm AG in the year 2002 as Application Specialist Titration. As Head of the Competence Center Titration he leads a team of Product Managers, Product Specialists and Application Chemists for all different titration techniques, pH and conductivity measurement, titration automation and software.
</blockquote>

Metrohm: How to Improve Your Titration Results – Optimizing Performance and Troubleshooting

How to Improve Your Titration Results – Optimizing Performance and Troubleshooting

How to get the basics of titration right so you can always trust your results.

How to Improve Your Titration Results & Troubleshooting

Have you ever wondered how the quality of your sensor can influence your titration results?
In this webinar, Dr. Sabrina Gschwind, sensors product manager at Metrohm, will detail proper sensor maintenance and how you can check and monitor the performance of your unit. In addition, you will receive tips and tricks to increase the quality of your titration results and thus avoid wasting time on tedious root cause analysis and instrument downtime due to sensor failure.
Key Learning Objectives:
<ul>
<li>Learn which component of a sensor has which function
</li>
<li>Learn how to clean, maintain, and store the most commonly used titration sensors
</li>
<li>Understand how the sensor can influence your titration results
</li>
<li>Gain the know-how to overcome the most common titration errors
</li>
</ul>
Who should attend?
Everybody involved in performing potentiometric or photometric titrations and who wishes to gain more in-depth knowledge about which sensors to use, sensor maintenance, storage, as well as tips and tricks for best titration results.
<blockquote>
Presenter: Sabrina Gschwind (Product Manager Sensors, Metrohm AG)
</blockquote>
<blockquote>
Sabrina has been working at Metrohm International Headquarters for almost 5 years, initially in the application lab developing tailor-made applications. Since 2016, Sabrina has been the product manager responsible for the Metrohm sensor portfolio, which includes pH, metal and ion-selective electrodes, and photometric sensors as well as conductivity measuring cells. In this role, she is not only taking care of the entire product lifecycle, but also provides support and training for sensors.
</blockquote>
<blockquote>
Presenter: Dr. Matthew McArdle (Editor, SelectScience)
</blockquote>
<blockquote>
Matthew McArdle is a member of the editorial team at SelectScience and plays a key role in content production, specializing in molecular biology and biochemistry fields. He has a Ph.D. from Leeds University, where he researched the effects of promoter-driven gene expression on vascular smooth muscle cell growth.
</blockquote>

Metrohm: Avoid titration mistakes through best practice sensor handling

Avoid titration mistakes through best practice sensor handling

Proper sensor maintenance and how you can check and monitor the performance of your unit.

Avoid titration mistakes via best practice sensor handling

Perfect and fully traceable titration results require maximum synergy between the titration hardware and the reagents used – this is especially required when working under GxP conditions or in a regulated environment.
Is the titration performed with the correct equipment and reagents? Do the titration hardware and reagents qualify for the intended application? Have there been any deviations from normal routine? And, maybe most importantly, how can this all be proved, immediately before or during the titration, as well as later in your archived titration data?
In this webinar, Eyla Reuss (Merck KGaA) and Christian Haider (Metrohm AG) introduce you to the advantages of the unique combination of the Omnis titration platform with the jointly developed 3S titration reagents. Find out how automated transfer of relevant data ensures data integrity and traceability.
By viewing this presentation you will learn...
<ul>
<li>how modern titration hardware improves safety in the lab and can prevent handling or application errors
</li>
<li>how modern reagents leave no data gap for the reagents used
</li>
<li>how modern titration software can simplify your documentation and maintain data security and integrity
</li>
<li>how easy it is to track titration data to the manufacturer’s certificates
</li>
</ul>
Who should view this webinar?
Lab chemists, laboratory managers, quality control professionals, pharmaceutical industry professionals or simply everybody that is interested in titration.
<blockquote>
Presenter: Eyla Reuss (Merck KGaA)
</blockquote>
<blockquote>
As Chemical Laboratory Technician in the Life Science R&amp;D department, Eyla Reuss joined Merck in 2012 and is now responsible for new developments of reagents, standards and devices in the field of titration and Karl Fischer titration.
</blockquote>
<blockquote>
Presenter: Christian Haider (Metrohm AG)
</blockquote>
<blockquote>
Dr. Haider joined Metrohm AG in 2002 as Application Specialist Titration. He leads the Competence Center Titration that is specialized on all different titration techniques, pH and conductivity measurement, titration automation and software.
</blockquote>

Titration and Lab 4.0

The advantages of the unique combination of the Omnis titration platform with the jointly developed 3S titration reagents.

Titration and Lab 4.0 (Full Traceability & Data Integrity)

Separation Science, in collaboration with Metrohm, has developed an on-demand webinar covering the benefits of automating sample preparation for titration.
Stand-alone potentiometric or Karl Fischer titrations are a full-time job for lab technicians, as they have to do sample preparation, start the titration, dispose of the titrated solution, and rinse the titration equipment, followed by re-conditioning of the electrode prior to the next titration if requested.
Sample preparation includes the addition of solvents and auxiliary solutions, aliquoting of liquid samples, dissolution or homogenization of solids, filtration, or even more complex techniques, e.g., time-controlled pre-reactions with auxiliary reagents prior to titration. In addition, other chemical or physical properties of the sample such as consistency, poor solubility, hygroscopy, or possible side reactions may contribute to inconsistent results.
This on-demand webinar presents options and examples of how sample preparation for potentiometric and Karl Fischer titration can be fully automated and, therefore, be made safer, more efficient and more reproducible. Furthermore, the webinar shows how the whole titration workflow can be made much faster.
By viewing this on-demand webinar you will...
<ul>
<li>Learn how automation can eliminate manual handling of toxic reagents and waste solutions.
</li>
<li>Understand how automation increases reproducibility because all sample preparation steps are performed automatically.
</li>
<li>Learn how automation saves time and money because parallel operation allows for a higher throughput and frees up laboratory staff.
</li>
</ul>
The presentation is ideal for...
<ul>
<li>Anyone who is interested in automated titration systems and would like to know more about it.
</li>
<li>Lab technicians who want to reduce human errors during sample preparation and increase the safety handling aggressive reagents.
</li>
<li>Lab managers who are interested in time and money that could be saved by working with automated systems.
</li>
</ul>
<blockquote>
Presenter: Heike Risse (Product Manager Titration Automation, Metrohm)
</blockquote>
<blockquote>
Heike is Product Manager Titration Automation in the Competence Center for Titration at Metrohm International Headquarters, Herisau, Switzerland. She has worked with titration for more than 18 years at Metrohm. During this time, Heike worked as an Application Specialist Titration with focus on method and application development for all industries. For more than 13 years, she has been responsible for the product management of automated titration systems.
</blockquote>

Metrohm: Improve Titration Reproducibility and Efficiency with Automation

Improve Titration Reproducibility and Efficiency with Automation

Options and examples of how sample preparation for potentiometric and Karl Fischer titration can be fully automated.

Titration Reproducibility and Efficiency with Automation

Water is a vital resource for refineries and petrochemical plants. Supporting processes that pre-treat crude oil, remove and recover sulfur, and prevent corrosion, catalyst poisoning, and fouling are vital to keep operations running efficiently and safely.
Maintaining optimal water chemistry requires many measurements for multiple analytes. We will discuss a variety of topics including: which technology provides the best measurement and throughput for your needs; what other automated techniques can be applied in place of traditional wet chemistry methods, such as titration, to reduce error and hazards; and how the combination of discrete analyzer and ion chromatography (Disc-IC) solves routine and comprehensive water analysis problems.
<blockquote>
Presenter: Hari Narayanan (Product Marketing Manager, Thermo Fisher Scientific)
</blockquote>
<blockquote>
Dr. Hari Narayanan has nearly 20 years of experience in managing research activities and applications development. He collaborates with regulatory agencies and has been part of several new method developments. He is well-versed in multivariate analysis, sample preparation, titration, ion chromatography, combustion IC, molecular spectroscopy (FTNIR, FTIR, RAMAN, and UV), and voltammetry. He received his B.Sc. and M.Sc. in Chemistry from the Madurai Kamaraj University and a Ph.D. in Analytical Chemistry from the University of Madras, India.
</blockquote>
<blockquote>
Presenter: Kirk Chassaniol (Manager, Application Scientists, Thermo Fisher Scientific)
</blockquote>
<blockquote>
Kirk is currently a technical leader in the Ion Chromatography (IC) field support group within the North American Centers of Excellence. Over the last 28 years, he has been involved in IC application and customer support in sales and marketing roles. He has helped with the development of IC methods and standards for chloride and sulfate in ethanol, cations in hydrogen cell feed gas, and combustion IC within the ASTM D02 and D03 Elemental Analysis subcommittee.
</blockquote>

Thermo Scientific: It Takes a Lot of Water to Turn Oil into Gold

It Takes a Lot of Water to Turn Oil into Gold

Variety of topics including: which technology provides the best measurement and throughput for your needs.

LCMS webinars focusing on Titration - page 5