Professor Michaela Rumlová, head of a research group of the National Institute of Virology and Bacteriology at UCT Prague (and Vice-Rector for doctoral studies), holder of a licensed patent &nbsp;for antiretroviral testing assays, and recipient of the Czech Science Foundation’s President’s Award for HIV research, has also become the principal investigator of the bilateral INTER-EXCELLENCE II project “Cellular factors regulating the export and packaging of unspliced ​​retroviral RNA”, starting this year. In this interview, she explains how retroviruses such as HIV “hack” the cell in order to export their own genome from the nucleus into the cytoplasm, and then to the site where it is specifically packaged into nascent viral particles, a key step in viral replication. Her team is collaborating with the NIH (National Institutes of Health) and Rockefeller University to elucidate mechanisms that may help reveal new vulnerable points in the retrovirus replication cycle while simultaneously clarifying related cellular processes.<h5>Could you describe your project and its main objectives?</h5>Retroviruses, such as HIV, are viruses that carry genetic information in the form of RNA; however, after entering a cell, they are able to reverse transcribe it into DNA and integrate it into the host genome. In order to replicate, they must transport their genetic information—in the form of unspliced RNA—from the nucleus into the cytosol. There, it serves both as a template for the production of viral proteins and as the genome of new viral particles. This is highly unusual, as the cell very carefully controls which RNA molecules are allowed to leave the nucleus. Normal protein-coding cellular RNAs, known as messenger RNAs (mRNAs), must undergo splicing—a processing step in which certain segments are removed—before being exported. When this process fails, mRNA that retains these segments is held back in the nucleus as part of quality control and subsequently degraded. Retroviruses have managed to outwit this cellular control machinery, allowing their unspliced RNA to pass into the cytoplasm.Our project focuses on identifying the cellular proteins that assist retroviruses in this step and on the mechanism by which protein complexes bound to viral RNA are remodelled during RNA transport. The goal is to understand how the virus uses cellular mechanisms to its advantage and how this pathway is linked to the subsequent fate of the RNA, i.e. to its translation and packaging into new viral particles.<h5>Why is this export of unspliced ​​RNA so important for retroviral replication?</h5>Because this RNA has a dual role for the virus. On the one hand, it serves as a template for synthesizing viral proteins in the cytoplasm; on the other, it represents the genetic material itself, which must be packaged into new viral particles. The virus must package its RNA genome in its unspliced form, as only this RNA contains essential sequences without which further viral replication would be impossible.Simply put: the virus needs to get a complete copy of its genetic instructions out of the nucleus. Once the viral RNA is in the cytoplasm, it can be used as a template for synthesizing viral proteins; and a portion of these RNA molecules is then selected and packaged into a new particle as a genome. We are interested in how the virus forces the cell to allow viral RNA that is, from the cell’s perspective, “unfinished,” that is unspliced, to leave the nucleus. We are also investigating how the cell and the virus together - albeit unintentionally from the cell’s perspective - determine which RNA molecule will be translated into proteins and which will be packaged as the genome. This is one of the key, yet still not fully understood, steps of retroviral replication.<h5>What do you think it was about your project that impressed the Ministry of Education, Youth and Sports’ jury the most?</h5>I believe the decisive factor was a combination of several elements. First, the reviewers were primarily interested in the scientific problem itself. We are investigating a mechanism that is essential for retroviruses, but still not sufficiently understood, which may also have broader implications for the regulation of cellular processes. In addition, the quality of the team, our strong collaboration with the NIH and Rockefeller University, and the use of modern methods that will allow us to track viral RNA in great detail also played a role. It was also important that the project has clear objectives and a realistic plan.<h5>How will this research contribute to the development of new treatments for retroviral infections?</h5>It is important to say that this is primarily basic research. Rather than developing a new drug directly, we are trying to understand, at the molecular level, the step that the virus cannot do without. Only when we fully understand the mechanism of these cellular processes and identify the essential factors for the export of unspliced ​​RNA and how they function, will we be able to better determine which interactions or mechanisms represent potentially vulnerable points. Similar mechanisms associated with the export of unspliced ​​mRNA also appear in some diseases, such as cancer, so I &nbsp;see the benefit of our research mainly in that it will refine our understanding of these pathological processes in the cell, and retroviruses help us do so. Therefore, our work on retroviruses not only shows us how the virus works, but also how the cell itself works.<h5>What responsibilities does your UCT Prague team have? How does the collaboration with your American partners work?</h5>At UCT Prague, we are conducting the project’s primary experimental work. Our research involves cell cultures, viral models, and various biochemical techniques ranging from immunochemistry to microscopy that allow us to track viral RNA and identify its cellular partners. In collaboration with colleagues from NIH, Frederick, we are developing single-particle tracking methods to observe viral RNA movement in living cells across both space and time. Furthermore, we are working with colleagues from Rockefeller University to employ iCLIP methods for the precise mapping of protein-binding sites on RNA. Both collaborations are valuable to us, because they are not just about data sharing, but also about the transfer of expertise and detailed know-how.<h5>Who else is collaborating on the project?</h5>At UCT Prague, we collaborate closely with the Department of Biotechnology and the Department of Biochemistry and Microbiology. Externally, we work with colleagues from the Institute of Organic Chemistry and Biochemistry of the CAS (IOCB Prague) and the Central European Institute of Technology in Brno (CEITEC). Our research naturally necessitates the integration of diverse fields of expertise; it requires combining virology, cell biology, and biochemistry with advanced imaging and modern approaches to study RNA-protein interactions. This is exactly what makes our project capable of addressing complex questions that a single laboratory cannot tackle on its own.<h5>Are there plans to continue the project? Have you brought on new collaborators, and are you looking to welcome new colleagues to the team?</h5>Yes, definitely. In basic research, it often happens that one answered question opens up several more. High-quality, properly interpreted data provide a foundation you can build on — and that is what moves scientists forward. It is already clear that the topic of retroviral RNA export and packaging has broader implications and may reveal more general principles of how RNA functions within the cell. We also hope the project will be attractive to students, as it connects classical molecular biology, biochemistry, and virology with cutting-edge biotechnological methods. Students are already involved in the project as part of their bachelor’s and master’s theses.<h5>What was the personal turning point that motivated you to pursue a long-term investigation into retroviral particles?</h5>I first encountered retroviruses in the early 1990s during my diploma thesis, when I was working at IOCB Prague on HIV-1 protease inhibitors. At that time, AIDS could not yet be effectively treated, and retrovirus research had a completely different dimension. From the very beginning, I was fascinated by how such a relatively small and simple virus can outsmart evolutionarily highly optimized cellular processes and turn them to its own advantage. And that has never stopped fascinating me. From a scientific point of view, retroviruses are remarkable; just consider one of their enzymes, reverse transcriptase, without which no diagnostic or research laboratory in the world could function today. Without it, it would be impossible to diagnose RNA viruses like SARS-CoV-2, influenza, or HIV using RT-PCR tests, or, for example to produce recombinant human proteins in bacterial cells.

Tracking unspliced RNA: Professor Rumlová studies how retroviruses “hack” cells

High-performance liquid chromatography (HPLC) remains one of the most widely used analytical techniques for separating, identifying, and quantifying compounds in complex mixtures. Successful HPLC method development depends not only on selecting the correct column and optimizing the mobile phase composition, but also on choosing the appropriate detector. Even a perfectly optimized chromatographic separation provides little value if the detection system cannot properly capture and interpret the analytes of interest.In many ways, the detector acts as the “vision system” of the chromatograph. It converts separated compounds into measurable electronic signals, allowing analysts to identify components and determine their concentrations. The choice of detector therefore has a major impact on sensitivity, selectivity, robustness, and the overall quality of analytical data.This article provides an overview of the most common HPLC detection techniques, discusses their strengths and limitations, and outlines the most important criteria to consider when selecting a detector for a specific analytical task.<h3>HPLC Detection Technologies</h3>HPLC detectors are typically divided into two major categories:<ol><li>Specific detectors (solute-property detectors)</li><li>Bulk property detectors</li></ol>Specific detectors respond to a particular chemical or physical property of the analyte. Ideally, their signal is largely unaffected by changes in mobile phase composition. Common examples include UV/Vis detectors, fluorescence detectors, and mass spectrometers.Bulk property detectors, on the other hand, measure differences between the pure mobile phase and the mobile phase containing analytes. These detectors are considered more universal because they respond to properties shared by many compounds. Refractive index detectors are among the best-known examples, alongside conductivity and light-scattering detectors.Both detector categories have important advantages depending on the application, sample type, and analytical goals.<h3>Common Detector Types Used in HPLC</h3>A wide variety of detectors are available for HPLC systems, ranging from highly universal devices to extremely selective and sensitive technologies.Common detector options include:<ul><li>UV/Vis detectors</li><li>Diode array detectors (DAD/PDA)</li><li>Fluorescence detectors</li><li>Refractive index detectors (RID)</li><li>Conductivity detectors</li><li>Evaporative light scattering detectors (ELSD)</li><li>Charged aerosol detectors (CAD)</li><li>Mass spectrometry detectors (MS)</li></ul>Each technology offers a different balance of sensitivity, selectivity, robustness, operational complexity, and cost.<img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/KNAUER_Detector_overview_with_characteristics_Graphic_by_KNAUER_84b0dd30b1.jpg" alt="KNAUER: Detector overview with characteristics. Graphic by KNAUER." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_KNAUER_Detector_overview_with_characteristics_Graphic_by_KNAUER_84b0dd30b1.jpg 245w," sizes="100vw" width="1293" height="596"><h3>Selecting the Right HPLC Detector</h3>There is no universally ideal detector for all HPLC applications. The best choice depends on several factors, including analyte chemistry, matrix complexity, required detection limits, and instrument compatibility.Careful detector selection is essential for obtaining reliable and meaningful analytical results.The following considerations can help guide the selection process.<h4>1. Chemical and Physical Properties of the Analytes</h4>The chemical nature of the compounds being analyzed is one of the most important selection criteria.Questions to consider include:<ul><li>Do the analytes contain chromophores?</li><li>Are the compounds naturally fluorescent?</li><li>What is their polarity?</li><li>What is their molecular weight?</li><li>Are they volatile or non-volatile?</li></ul>Compounds containing UV-active groups are often well suited for UV/Vis detection, while fluorescent molecules can benefit from the high sensitivity of fluorescence detectors. Non-UV-active compounds may require universal detection approaches such as CAD, ELSD, or mass spectrometry.<h4>2. Required Sensitivity</h4>Detector sensitivity determines the minimum concentration that can be reliably detected and quantified.Routine quality control analyses often rely on UV/Vis detectors because they provide sufficient sensitivity for many applications while remaining relatively simple and cost-effective. However, trace-level analysis or highly complex matrices may require more advanced technologies such as:<ul><li>Fluorescence detection</li><li>Charged aerosol detection</li><li>Mass spectrometry</li></ul>These techniques offer significantly lower detection limits and greater analytical specificity.<h4>3. Detector Linearity</h4>Linearity describes how well detector response correlates with analyte concentration across a defined range.A detector with strong linearity ensures accurate quantification and simplifies calibration procedures. Poor linearity can introduce quantification errors, especially when analyzing samples across broad concentration ranges.<h4>4. Dynamic Range</h4>Dynamic range refers to the concentration interval over which the detector can reliably measure analytes.Applications involving samples with highly variable concentrations require detectors capable of maintaining accurate response over a broad range. A wide dynamic range reduces the need for repeated dilutions or multiple analytical runs.<h4>5. Selectivity</h4>Selectivity defines the detector’s ability to distinguish target analytes from other matrix components.This factor becomes particularly important for complex samples containing many interfering substances. Highly selective detectors reduce the risk of overlapping signals and false positives.Mass spectrometry is often advantageous when dealing with unknown compounds or highly complicated sample matrices because it provides structural information in addition to quantitative data.<h4>6. Budget Considerations</h4>Detector cost is another practical factor that laboratories must evaluate.UV/Vis and refractive index detectors are generally among the more economical choices, making them attractive for routine analyses and standard applications.More sophisticated technologies such as fluorescence detection, CAD, or mass spectrometry typically require:<ul><li>Higher initial investment</li><li>Additional maintenance</li><li>More complex infrastructure</li><li>Greater operational expertise</li></ul>However, the overall value of the detector should always be considered in relation to analytical performance requirements and long-term application needs.<h4>7. Instrument and Mobile Phase Compatibility</h4>The selected detector must be compatible with the operational conditions of the HPLC system.Important parameters include:<ul><li>Flow rate range</li><li>Pressure limitations</li><li>Mobile phase composition</li><li>Solvent compatibility</li></ul>Some detectors are highly sensitive to mobile phase composition or gradient changes, while others tolerate a broader range of operating conditions.Ensuring proper compatibility helps prevent baseline instability, signal interference, and reduced detector performance.<h4>8. Destructive vs. Non-Destructive Detection</h4>Another important consideration is whether the analytical workflow involves only detection or also preparative purification.Non-destructive detectors allow collected fractions to remain intact after detection, which is highly beneficial for preparative chromatography and purification workflows.Destructive detectors, by contrast, consume or alter the sample during measurement and are therefore more suitable for analytical applications where sample recovery is not required.<h4>9. Reliability and Ease of Operation</h4>Practical usability is often just as important as analytical performance.Some detectors are designed for straightforward plug-and-play operation, while others require:<ul><li>Frequent calibration</li><li>Specialized maintenance</li><li>Dedicated gas supplies</li><li>Vacuum systems</li><li>Advanced operator expertise</li></ul>Laboratories should evaluate the available technical support, user experience, and maintenance capabilities before implementing more sophisticated detection technologies.Reliable operation and manageable maintenance requirements can significantly improve laboratory productivity and reduce downtime.Choosing the correct HPLC detector starts with understanding both the sample and the analytical objective. Rather than selecting a detector simply because it is available, laboratories should ensure that the technology aligns with the chemistry of the analytes, required performance characteristics, and workflow demands.In many cases, combining multiple detectors within a single HPLC system can provide even greater analytical insight by delivering complementary information from the same chromatographic separation.<img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/KNAUER_Flow_scheme_for_detector_selection_Graphic_by_KNAUER_486004045f.jpg" alt="KNAUER: Flow scheme for detector selection. Graphic by KNAUER." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_KNAUER_Flow_scheme_for_detector_selection_Graphic_by_KNAUER_486004045f.jpg 245w," sizes="100vw" width="1316" height="808">Interested in learning more? Future articles will explore individual HPLC detection techniques in greater detail, including their operating principles, application areas, and practical advantages for different analytical workflows.

A comprehensive guide for selecting the appropriate HPLC detector

News from LabRulezLCMS Library - Week 20, 2026

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The University of Chemistry and Technology, Prague is a natural centre of first-rate study and research in the area of chemistry in Czechia and is one of the country's largest educational and research institutions focused on technical chemistry, chemical and biochemical technologies, material and chemical engineering, food chemistry, and environmental studies.

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Based in Berlin, KNAUER is a medium-sized, owner-managed company that has been serving the sciences since 1962. We develop and manufacture scientific instruments of superior quality for liquid chromatography, including: Analytical HPLC/UHPLC, Preparative HPLC, Fast protein liquid chromatography (FPLC), Multi-column chromatography/Simulated moving bed (SMB) chromatography. High pressure dosing, in-line detection systems, and osmometry are additional business areas.

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Monitoring the quality of hydraulic and/or lube oils is critical to prevent premature wear of any kind of machinery. In this session we will look at this topic from a new perspective focusing on the combination of titration (potentiometric, thermometric, coulometric Karl Fischer titration) and Vis-Near-Infrared Spectroscopy (Vis-NIRS).
Combining titration (highly sensitive, specific, a widely used primary method) with Vis-NIRS (fast, non-destructive, results for multiple parameters at once) can make your oil condition monitoring and/or lubricant analysis much more efficient, especially, if your laboratory has to analyze large sample series every day.
Attending this session, you will learn and understand:
<ul>
<li>The specific advantages of each method (titration and Vis-NIR-Spectroscopy)</li>
<li>How and when to use these methods to measure critical chemical and physical parameters in lube and hydraulic oils</li>
<li>What thermometric titration is and how it helps to improve robustness of serial acid number testing</li>
<li>How to automate measurements of large sample series to increase efficiency and reproducibility</li>
<li>How Vis-NIRS for lubricant analysis complies with ASTM guidelines and can save costs and time</li>
</ul>
<blockquote>
Presenter: Robert Tobler (Application Specialist Titration)
</blockquote>
<blockquote>
Robert Tobler studied chemistry at the Zurich University of Applied Science in Wädenswil, Switzerland. He joined Metrohm in 2015 as an application specialist titration. Since 2018 he has also been involved in stability measurement.
</blockquote>
<blockquote>
Presenter: Nicolas Rühl, PhD (Product Manager Spectroscopy)
</blockquote>
<blockquote>
Dr. Nicolas Rühl is working in the Competence Center Spectroscopy since 2015. In this time, he has been in charge of developing the near-infrared (NIR) spectroscopy business at Metrohm. Nicolas has a Master in Business Administration and received his PhD in physical chemistry on single-molecule spectroscopy using Vis-NIR and Raman spectroscopy technologies.
</blockquote>
<blockquote>
Moderator: Tom Lynch CChem FRSC (Independent Analytical Consultant, International Labmate)
</blockquote>
<blockquote>
Tom has 35 years of experience in the petroleum industry, specialising in the delivery of forensic and problem solving /method development capabilities for BP Fuels and Lubricants businesses globally. He was also a member of the BP Science Council representing Analytical Science and led a BP wide Analytical Science network.Tom has published over 30 citable papers, 4 book chapters and has given over 60 presentations at conferences. He is a past Vice President of the Royal Society of Chemistry (RSC) Analytical Division and a past Chairman of the RSC Separation Science Group. In addition, Tom is a recipient of the Silver Jubilee Medal by the Chromatographic Society and a technical achievement award by the Energy Institute.
</blockquote>

Metrohm: Making your quality control of hydraulic and lube oils more efficient

Making your quality control of hydraulic and lube oils more efficient

Combining titration and Vis-Near-Infrared Spectroscopy for higher sample throughput & faster results

Efficient quality control of hydraulic and lube oils

Increasing numbers of samples, multiple parameters to be determined, mandatory compliance with national and international standards – water works, bottlers of mineral water and some sectors of the process industry all face the same challenge: How to cope with more analytical tasks while human resources in the laboratory are limited?
In this eLearning session we demonstrate how automation can help you keep pace with today’s complex QC requirements in the water industry. Learn how all your standard measurements and titrations but also parameters like turbidity, color, and all critical ions can be consolidated on a modular, fully automated analytical platform – including dedicated sample preparation. Best practice examples are given from modern water laboratories that were able to achieve time savings of up to 60% while safeguarding robust, consistent results of their daily routine analytics.
<h5>Key Learning Objectives</h5>
<ul>
<li>How to consolidate typical QC applications from the water industry on a fully automated analytical platform</li>
<li>How much time can be freed up performing multiple different analytical measurements in parallel</li>
<li>How automated sample preparation can be seamlessly integrated on such a platform</li>
<li>How automated systems can increase reproducibility of results</li>
</ul>
<h5>Who should attend this eLearning?</h5>
<ul>
<li>Water authorities responsible for water quality monitoring</li>
<li>Managers and experts from environmental water testing laboratories</li>
<li>Managers and experts in charge of industrial process water monitoring</li>
</ul>
<blockquote>
Presenter: Heike Risse (Product Manager Titration Automation, Metrohm AG)
</blockquote>
<blockquote>
Heike is Product Manager Titration Automation at the Competence Center for Titration at Metrohm International Headquarters, Herisau, Switzerland. She has more than 21 years of work experience with titration. During her time with Metrohm, Heike used to work as an Application Specialist Titration with focus on method and application development for virtually every industry sector. For more than 16 years, she has been responsible for the product management of automated titration systems.
</blockquote>
<blockquote>
Presenter: Jennifer Lüber (Product Specialist Titration, Metrohm AG)
</blockquote>
<blockquote>
Jennifer Lüber is the Product Specialist for Titration/TitrIC at Metrohm AG in Switzerland. She finished her apprenticeship as a Lab Technician at Metrohm AG in 2014. Afterwards, she was involved in projects related to column development, and worked in the laboratory for a short time at Metrohm UK. In 2015, Jennifer returned to Metrohm AG to join the competence center for titration, eventually taking responsibility for the TitrIC systems in 2018. Jennifer received her bachelor’s degree in business process management in 2020.
</blockquote>
<blockquote>
Moderator: Tom Lynch CChem FRSC (Independent Analytical Consultant, International Labmate)
</blockquote>
<blockquote>
Tom has 35 years of experience in the petroleum industry, specialising in the delivery of forensic and problem solving /method development capabilities for BP Fuels and Lubricants businesses globally. He was also a member of the BP Science Council representing Analytical Science and led a BP wide Analytical Science network.Tom has published over 30 citable papers, 4 book chapters and has given over 60 presentations at conferences. He is a past Vice President of the Royal Society of Chemistry (RSC) Analytical Division and a past Chairman of the RSC Separation Science Group. In addition, Tom is a recipient of the Silver Jubilee Medal by the Chromatographic Society and a technical achievement award by the Energy Institute.
</blockquote>

Metrohm: Water analysis – automation as a viable approach to increase efficiency and reproducibility

Water analysis – automation as a viable approach to increase efficiency and reproducibility

In this eLearning session we demonstrate how automation can help you keep pace with today’s complex QC requirements in the water industry.

Water analysis - automation & efficiency & reproducibility

Karl Fischer titration is a widely used analytical method in quality control and research &amp; development. Accuracy and precision of the results are critical to ensure the efficacy of process and final products.
Weighing is an integral step of analytical measurements and determinations in all laboratories. As weighing forms the first stage of the analysis chain, accuracy and precision of the eventual results would be highly influenced by the reliability of the weight. 
<h5>Key Learnings:</h5>
Join us in this webinar by Metrohm and Sartorius to learn about: 
<ul>
<li>The factors affecting accuracy and precision in Karl Fischer titration</li>
<li>Fundamentals in weighing</li>
<li>System integration for titrator and balance</li>
<li>Compliant documentation of the weighing process (FDA CFR 21 Part 11)</li>
</ul>
<blockquote>
Presenter: James Palmer (Field Application Specialist, Sartorius Corporation, USA)
</blockquote>
<blockquote>
James has worked in the weighing industry for 30 years. He has been with Sartorius for 10 years as the lead Metrologist for North America and Weighing Specialist for both the West and East Coast. Prior to Sartorius Jim worked for distribution in the weighing industry for 20 years performing service and sales of weighing devices.
</blockquote>
<blockquote>
Presenter: Michael Margreth (Senior Product Specialist Titration, Metrohm International Headquarters, Switzerland)
</blockquote>
<blockquote>
Michael Margreth studied chemistry at the University of Applied Science in Muttenz, Switzerland, before joining Metrohm in 2005 as an application specialist where he gained valuable titration experience in the application laboratory. Since 2008, he has mainly focused on Karl Fischer titration and the determination of water.
</blockquote>
<blockquote>
Moderator: Róisín Murtagh (Editor Wiley Analytical Science, Wiley)
</blockquote>
<blockquote>
Róisín Murtagh is an editor for Wiley Analytical Science Magazine. She studied science at Maynooth University, Ireland, and has an MSc in Medicinal Chemistry from the Open University, UK. She has over 10 years of analytical science experience from both industry and academic research.
</blockquote>

Wiley Analytical Science: Weigh It Right the First Time in Karl Fischer Titration

Weigh It Right the First Time in Karl Fischer Titration

Karl Fischer titration is a widely used analytical method in quality control and research & development. Accuracy and precision of the results are critical.

Titration is one of the most basic analytical methods for chemical analysis.
However, even power users sometimes struggle with the quality of their titrations. In this expert webinar, we explore the basics of volumetric titration and present tips and advice to help you make sure that your titration results are always precise and reproducible. The following topics will be addressed:
What is the most suitable titration method for my sample or sample matrix?
<ul>
<li>Photometric vs. potentiometric titration
</li>
<li>Direct vs. back (residual) titration
</li>
<li>End point vs. equivalent point titration
</li>
</ul>
Hardware selection
<ul>
<li>Sensor/burette size/stirrer</li>
</ul>
Reagent selection
<ul>
<li>Buffer solutions/titrant/standard reference reagents</li>
</ul>
Method development &amp; validation
<ul>
<li>Titration modes &amp; parameters
</li>
<li>Method validation (selectivity, linearity, accuracy &amp; reproducibility)
</li>
</ul>
Key learning objectives
<ul>
<li>How to select the proper hardware and the right chemistry for your titrations
</li>
<li>How to optimize titration performance depending on your application
</li>
<li>How to verify the suitability and accuracy of a titration method
</li>
<li>How to convert manual titration methods into automated procedures
</li>
</ul>
Who should attend?
This webinar will be of particular interest to people in charge of developing or qualifying volumetric titrations, especially lab technicians, lab managers and quality assurance professionals
<blockquote>
Presenter: Dr. Christian Haider (Head of Competence Center Titration, Metrohm AG)
</blockquote>
<blockquote>
Dr. Christian Haider joined Metrohm AG in the year 2002 as Application Specialist Titration. As Head of the Competence Center Titration he leads a team of product managers, product specialists and application chemists for all different titration techniques, pH and conductivity measurement, titration automation and software.
</blockquote>
<blockquote>
Presenter: Angela Schwehn (Chemical Laboratory Technician, Merck KGaA, Darmstadt, Germany)
</blockquote>
<blockquote>
Angela Schwehn is a chemical laboratory technician in the Life Science R&amp;D department at Merck KGaA, Darmstadt, Germany. She has more than 15 years of experience in modern volumetric titration and Karl Fischer titration and is responsible for the development of new reagents and standards in the field of volumetric titration and Karl Fischer titration.
</blockquote>
<blockquote>
Presenter: Sarah Thomas (Editorial Team, SelectScience)
</blockquote>
<blockquote>
Sarah studied biology at the University of Bath, UK, and has worked in the Pharmaceutical Sciences Department of St. Jude Children’s Research Hospital, Memphis. As a member of the Editorial team, Sarah plays an integral role in shaping the content on SelectScience.
</blockquote>
<blockquote>
Presenter: Ellie Abbott (Editorial Team, SelectScience)
</blockquote>
<blockquote>
Ellie studied biochemistry at the University of Nottingham, UK, and has previous experience working in management consultancy. As a member of the Editorial team, she now plays an integral role in shaping the content on SelectScience.
</blockquote>

Metrohm: Volumetric titration: A step-by-step guide to correct results

Volumetric titration: A step-by-step guide to correct results

The basics of volumetric titration and present tips and advice to help you make sure that your titration results are always precise and reproducible.

Volumetric titration: A step-by-step guide to correct result

In most laboratories, the age of manual titrations is over. Good so: Modern, digital autotitrators support users with various features that help reduce errors and optimize titration results.
However, even routine users are sometimes struggling with the basics, e.g., which kind of electrode to choose for which titration, which titration mode to select for which matrix, or how to make sure results are properly evaluated by the device.
In this presentation, we focus on how to get the basics of titration right so you can always trust your results:
<ul>
<li>How to select the right hardware for your titration (electrode, burette size, stirrer)
</li>
<li>How to handle your hardware properly (this is critical!)
</li>
<li>How to choose the correct settings and parameters on your autotitrator, depending on your matrix and method
</li>
<li>How endpoints are evaluated correctly and how to optimize endpoint evaluation
</li>
<li>Troubleshooting: What to do in case you get incorrect results
</li>
</ul>
This presentation is intended for all users of potentiometric titrators, chemists developing titration applications, laboratory managers – simply everyone who is interested in modern titration.
<blockquote>
Presenter: ChristianHaiderWebDr. Christian Haider (Head of Competence Center Titration, Metrohm AG)
</blockquote>
<blockquote>
Dr. Haider joined Metrohm AG in the year 2002 as Application Specialist Titration. As Head of the Competence Center Titration he leads a team of Product Managers, Product Specialists and Application Chemists for all different titration techniques, pH and conductivity measurement, titration automation and software.
</blockquote>

Metrohm: How to Improve Your Titration Results – Optimizing Performance and Troubleshooting

How to Improve Your Titration Results – Optimizing Performance and Troubleshooting

How to get the basics of titration right so you can always trust your results.

How to Improve Your Titration Results & Troubleshooting

Have you ever wondered how the quality of your sensor can influence your titration results?
In this webinar, Dr. Sabrina Gschwind, sensors product manager at Metrohm, will detail proper sensor maintenance and how you can check and monitor the performance of your unit. In addition, you will receive tips and tricks to increase the quality of your titration results and thus avoid wasting time on tedious root cause analysis and instrument downtime due to sensor failure.
Key Learning Objectives:
<ul>
<li>Learn which component of a sensor has which function
</li>
<li>Learn how to clean, maintain, and store the most commonly used titration sensors
</li>
<li>Understand how the sensor can influence your titration results
</li>
<li>Gain the know-how to overcome the most common titration errors
</li>
</ul>
Who should attend?
Everybody involved in performing potentiometric or photometric titrations and who wishes to gain more in-depth knowledge about which sensors to use, sensor maintenance, storage, as well as tips and tricks for best titration results.
<blockquote>
Presenter: Sabrina Gschwind (Product Manager Sensors, Metrohm AG)
</blockquote>
<blockquote>
Sabrina has been working at Metrohm International Headquarters for almost 5 years, initially in the application lab developing tailor-made applications. Since 2016, Sabrina has been the product manager responsible for the Metrohm sensor portfolio, which includes pH, metal and ion-selective electrodes, and photometric sensors as well as conductivity measuring cells. In this role, she is not only taking care of the entire product lifecycle, but also provides support and training for sensors.
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Presenter: Dr. Matthew McArdle (Editor, SelectScience)
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Matthew McArdle is a member of the editorial team at SelectScience and plays a key role in content production, specializing in molecular biology and biochemistry fields. He has a Ph.D. from Leeds University, where he researched the effects of promoter-driven gene expression on vascular smooth muscle cell growth.
</blockquote>

Metrohm: Avoid titration mistakes through best practice sensor handling

Avoid titration mistakes through best practice sensor handling

Proper sensor maintenance and how you can check and monitor the performance of your unit.

Avoid titration mistakes via best practice sensor handling

Perfect and fully traceable titration results require maximum synergy between the titration hardware and the reagents used – this is especially required when working under GxP conditions or in a regulated environment.
Is the titration performed with the correct equipment and reagents? Do the titration hardware and reagents qualify for the intended application? Have there been any deviations from normal routine? And, maybe most importantly, how can this all be proved, immediately before or during the titration, as well as later in your archived titration data?
In this webinar, Eyla Reuss (Merck KGaA) and Christian Haider (Metrohm AG) introduce you to the advantages of the unique combination of the Omnis titration platform with the jointly developed 3S titration reagents. Find out how automated transfer of relevant data ensures data integrity and traceability.
By viewing this presentation you will learn...
<ul>
<li>how modern titration hardware improves safety in the lab and can prevent handling or application errors
</li>
<li>how modern reagents leave no data gap for the reagents used
</li>
<li>how modern titration software can simplify your documentation and maintain data security and integrity
</li>
<li>how easy it is to track titration data to the manufacturer’s certificates
</li>
</ul>
Who should view this webinar?
Lab chemists, laboratory managers, quality control professionals, pharmaceutical industry professionals or simply everybody that is interested in titration.
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Presenter: Eyla Reuss (Merck KGaA)
</blockquote>
<blockquote>
As Chemical Laboratory Technician in the Life Science R&amp;D department, Eyla Reuss joined Merck in 2012 and is now responsible for new developments of reagents, standards and devices in the field of titration and Karl Fischer titration.
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Presenter: Christian Haider (Metrohm AG)
</blockquote>
<blockquote>
Dr. Haider joined Metrohm AG in 2002 as Application Specialist Titration. He leads the Competence Center Titration that is specialized on all different titration techniques, pH and conductivity measurement, titration automation and software.
</blockquote>

Titration and Lab 4.0

The advantages of the unique combination of the Omnis titration platform with the jointly developed 3S titration reagents.

Titration and Lab 4.0 (Full Traceability & Data Integrity)

Separation Science, in collaboration with Metrohm, has developed an on-demand webinar covering the benefits of automating sample preparation for titration.
Stand-alone potentiometric or Karl Fischer titrations are a full-time job for lab technicians, as they have to do sample preparation, start the titration, dispose of the titrated solution, and rinse the titration equipment, followed by re-conditioning of the electrode prior to the next titration if requested.
Sample preparation includes the addition of solvents and auxiliary solutions, aliquoting of liquid samples, dissolution or homogenization of solids, filtration, or even more complex techniques, e.g., time-controlled pre-reactions with auxiliary reagents prior to titration. In addition, other chemical or physical properties of the sample such as consistency, poor solubility, hygroscopy, or possible side reactions may contribute to inconsistent results.
This on-demand webinar presents options and examples of how sample preparation for potentiometric and Karl Fischer titration can be fully automated and, therefore, be made safer, more efficient and more reproducible. Furthermore, the webinar shows how the whole titration workflow can be made much faster.
By viewing this on-demand webinar you will...
<ul>
<li>Learn how automation can eliminate manual handling of toxic reagents and waste solutions.
</li>
<li>Understand how automation increases reproducibility because all sample preparation steps are performed automatically.
</li>
<li>Learn how automation saves time and money because parallel operation allows for a higher throughput and frees up laboratory staff.
</li>
</ul>
The presentation is ideal for...
<ul>
<li>Anyone who is interested in automated titration systems and would like to know more about it.
</li>
<li>Lab technicians who want to reduce human errors during sample preparation and increase the safety handling aggressive reagents.
</li>
<li>Lab managers who are interested in time and money that could be saved by working with automated systems.
</li>
</ul>
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Presenter: Heike Risse (Product Manager Titration Automation, Metrohm)
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Heike is Product Manager Titration Automation in the Competence Center for Titration at Metrohm International Headquarters, Herisau, Switzerland. She has worked with titration for more than 18 years at Metrohm. During this time, Heike worked as an Application Specialist Titration with focus on method and application development for all industries. For more than 13 years, she has been responsible for the product management of automated titration systems.
</blockquote>

Metrohm: Improve Titration Reproducibility and Efficiency with Automation

Improve Titration Reproducibility and Efficiency with Automation

Options and examples of how sample preparation for potentiometric and Karl Fischer titration can be fully automated.

Titration Reproducibility and Efficiency with Automation

Water is a vital resource for refineries and petrochemical plants. Supporting processes that pre-treat crude oil, remove and recover sulfur, and prevent corrosion, catalyst poisoning, and fouling are vital to keep operations running efficiently and safely.
Maintaining optimal water chemistry requires many measurements for multiple analytes. We will discuss a variety of topics including: which technology provides the best measurement and throughput for your needs; what other automated techniques can be applied in place of traditional wet chemistry methods, such as titration, to reduce error and hazards; and how the combination of discrete analyzer and ion chromatography (Disc-IC) solves routine and comprehensive water analysis problems.
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Presenter: Hari Narayanan (Product Marketing Manager, Thermo Fisher Scientific)
</blockquote>
<blockquote>
Dr. Hari Narayanan has nearly 20 years of experience in managing research activities and applications development. He collaborates with regulatory agencies and has been part of several new method developments. He is well-versed in multivariate analysis, sample preparation, titration, ion chromatography, combustion IC, molecular spectroscopy (FTNIR, FTIR, RAMAN, and UV), and voltammetry. He received his B.Sc. and M.Sc. in Chemistry from the Madurai Kamaraj University and a Ph.D. in Analytical Chemistry from the University of Madras, India.
</blockquote>
<blockquote>
Presenter: Kirk Chassaniol (Manager, Application Scientists, Thermo Fisher Scientific)
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<blockquote>
Kirk is currently a technical leader in the Ion Chromatography (IC) field support group within the North American Centers of Excellence. Over the last 28 years, he has been involved in IC application and customer support in sales and marketing roles. He has helped with the development of IC methods and standards for chloride and sulfate in ethanol, cations in hydrogen cell feed gas, and combustion IC within the ASTM D02 and D03 Elemental Analysis subcommittee.
</blockquote>

Thermo Scientific: It Takes a Lot of Water to Turn Oil into Gold

It Takes a Lot of Water to Turn Oil into Gold

Variety of topics including: which technology provides the best measurement and throughput for your needs.

<div id="text">Karl Fischer Titration is the preferred method for the determination of the water content and the reference method in many industries. Safe Karl Fischer titration is therefore an important topic.&nbsp;The safety of a Karl Fischer titration is influenced by several important factors:<ul style="margin-top:0px;"><li>Selection of the correct titrant and working medium</li><li>Selection of the correct certified standard for titer determination and system checks</li><li>Monitoring of the titration system to ensure its proper function</li><li>The Karl-Fischer titration itself – never miss the right moment when to start the titration</li><li>Replacement of the working medium when it is exhausted or when the titration vessel is full</li></ul>OMNIS – the new titration platform from Metrohm and Hydranal’s NEXTGEN reagents from Honeywell offer the most advanced solution for this.<h5>Co-Host Metrohm</h5>By attending this webinar, you will learn…<ul><li>How to carry out a Karl Fischer titration safely with reagents which are free of CMR (carcinogenic, mutagenic and reprotoxic) substances or halogenated hydrocarbons</li><li>How modern Karl Fischer titrators improve safety in the lab</li><li>How OMNIS titrator can help to achieve perfect results</li></ul><blockquote>Presenter: Dr. Roman Neufeld (Senior Research Scientist, Honeywell)<div>Roman joined Honeywell in 2017 as an Application Chemist, providing support to a global customer base on technical queries and application development for Karl Fischer titration. Since 2018, he has expanded his role to include development of new and innovative Hydranal reagents to stay ahead of market needs.</div>Presenter: Michael Margreth (Senior Product Specialist Titration, Metrohm Schweiz AG)<div>Michael Margreth is a Senior Product Specialist for Karl Fischer Titration at Metrohm International Headquarters in Herisau, Switzerland. Previously, he worked as Application Specialist for Titration at Metrohm AG. He studied chemistry at the University of Basel, Switzerland and at the FHBB in Muttenz, Switzerland. He obtained his degree in chemistry and joined Metrohm in 2005.</div></blockquote></div>

Honeywell: Safer Karl Fischer Titrations with CMR-free reagents and OMNIS

Safer Karl Fischer Titrations with CMR-free reagents and OMNIS

KF Titration is the preferred method for the determination of the water content and the reference method in many industries. 

LCMS webinars focusing on Titration - page 5