Ask any analytical chemist which hyphenated technique has become indispensable over the past decade, and the answer will almost certainly be the same: tandem chromatography–mass spectrometry. Whether it is <a target="_blank" rel="noopener noreferrer" href="https://www.organomation.com/preparing-samples-for-lc-ms/ms-analysis">LC–MS/MS</a> quantifying a novel biologic’s metabolic fate, or <a target="_blank" rel="noopener noreferrer" href="https://www.organomation.com/preparing-samples-for-gc-ms/ms-analysis">GC–MS/MS</a> chasing per- and polyfluoroalkyl substances (PFAS) down to sub-part-per-trillion levels in municipal water, the combination of chromatographic separation with the specificity of tandem mass detection has become the analytical workhorse of choice across virtually every regulated sector.Below we examine the key forces propelling this expansion, from biopharma pipeline dynamics and PFAS regulatory mandates to AI-driven automation and reshoring of pharmaceutical manufacturing—and explore what each trend means for laboratories managing sample preparation and analytical workflows.<h3>Biopharma: The Engine Room of LC–MS/MS Demand</h3><h4>GLP-1 Agonists and the Metabolomics Gold Rush</h4>The explosive commercial and scientific interest in GLP-1 receptor agonists—<a target="_blank" rel="noopener noreferrer" href="https://www.sepscience.com/advancing-glp-1-peptide-analysis-chromatography-challenges-and-innovations-12163">semaglutide and related peptide therapeutics </a>for diabetes and obesity—has dramatically expanded the analytical demands placed on tandem MS platforms. Discovery programs require precise quantification of peptide drug candidates and endogenous biomarkers across complex biological matrices. These workflows rely on high-sensitivity LC–MS/MS for pharmacokinetic profiling, receptor occupancy studies, and metabolomics characterization. Metabolomics is, in fact, projected to be the fastest-growing application segment in mass spectrometry, driven in part by its growing role in cancer screening, biomarker discovery, and metabolic disease research.I have been spending more time learning about GLP-1s because this is the hot topic that people want to discuss on the Concentrating on <a target="_blank" rel="noopener noreferrer" href="https://open.spotify.com/show/1wZg67rCjoGju4yhqxI3tL?si=n9GLz9zjSCOrVfXPxFPhng&amp;nd=1&amp;dlsi=52868e027aad4d22">Chromatography podcast</a>. With that said, I was astounded looking at CNBC.com during breakfast this morning to see that <a target="_blank" rel="noopener noreferrer" href="https://www.cnbc.com/2026/03/21/glp-1-diets-restaurants-protein-fiber-weight-loss-drugs.html">one in eight Americans are currently taking one of these drugs such as Ozempic</a>.<h4>Antibody–Drug Conjugates and Gene Therapy: Complexity at Every Step</h4>The ascent of antibody–drug conjugates (ADCs) and gene therapies has introduced a new level of analytical complexity. ADCs blend the target specificity of monoclonal antibodies with the cytotoxic potency of small-molecule payloads—and characterizing the linker chemistry, drug-to-antibody ratio (DAR), and conjugate stability demands high-resolution LC–MS/MS at every stage from discovery through QC release testing. Gene therapy vectors require equally rigorous characterization of impurities, aggregates, and empty/full capsid ratios.This translates into a direct need for low-evaporation, high-recovery concentration methods that preserve labile protein structures and maintain sample integrity prior to LC–MS/MS injection. <a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/article/5392">Nitrogen evaporation workflows</a> that minimize oxidative stress and maintain sample temperature are increasingly critical in these pipelines.<h4>Precision Medicine and Multi-Omics Integration</h4>The shift toward precision medicine has accelerated adoption of high-throughput, multi-analyte MS platforms. Proteomics, metabolomics, and lipidomics workflows are converging on a single analytical platform—tandem MS—and generating datasets of a scale and complexity that were unimaginable ten years ago.Make no mistake: the triple quadrupole serves an irreplaceable role in quantitative targeted analysis. At the same time, <a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/products?category=134&amp;manufacturerItems=3">Orbitrap </a>and Q–TOF hybrid platforms are growing rapidly as researchers demand full-scan accurate mass for untargeted discovery.<h3>Environmental Mandates: PFAS Testing Drives Adoption</h3>Environmental testing is something that every person, chemist or not, can understand the importance of. Mounting mandates from the U.S. Environmental Protection Agency and European regulators regarding PFAS—the “forever chemicals” whose extraordinary environmental persistence is matched only by the analytical challenge of detecting them at trace levels—have created immediate, compliance-driven investment in LC–MS/MS infrastructure.<h4>The Regulatory Framework Chemists Need to Know</h4>The EPA has codified a suite of LC–MS/MS-based methods as the analytical standard for PFAS in water and solid matrices:<ul><li><a target="_blank" rel="noopener noreferrer" href="https://blog.organomation.com/blog/epa-533-drying-down-pfas-samples-after-spe">EPA Method 533</a>: Weak anion exchange SPE combined with LC–MS/MS; covers 25 PFAS including short-chain compounds in drinking water.</li><li><a target="_blank" rel="noopener noreferrer" href="https://blog.organomation.com/blog/sample-concentration-epa-method-537-pfas-determination">EPA Method 537.1</a>: Reversed-phase SPE followed by LC–MS/MS; covers 18 PFAS including the GenX compound, with method detection limits in the low ng/L range.</li><li><a target="_blank" rel="noopener noreferrer" href="https://www.agilent.com/en/solutions/environmental/water-testing/pfas-in-water/us-epa-method-8327?srsltid=AfmBOooCY1pcavj7B1PH-75d3HO4BGDglNlhmyTyrpDY3zvUksMO1dlg">EPA Method 8327</a>: LC–MS/MS for selected PFAS in prepared extracts of aqueous and solid matrices.</li><li><a target="_blank" rel="noopener noreferrer" href="https://blog.organomation.com/blog/preparing-diverse-sample-matrices-for-epa-method-1633">EPA Draft Method 1633</a>: The emerging benchmark for PFAS in non-aqueous matrices—aqueous, solid, biosolids, and tissue—quantifying up to 40 PFAS.</li></ul>All of these methods designate LC–MS/MS, specifically LC coupled to triple quadrupole or tandem-in-time platforms operating in negative electrospray ionization mode with multiple reaction monitoring (MRM), as the required or recommended analytical technique. PFAS analysis is, at its core, an LC–MS/MS problem.<h4>The Sample Preparation Bottleneck</h4>Chemists who have worked with PFAS methods understand the particular demands they place on sample preparation. The ubiquity of<a target="_blank" rel="noopener noreferrer" href="https://blog.organomation.com/blog/tackling-pfas-contamination-practical-insights-from-epa-537.1-method-development"> PFAS in laboratory materials</a>—PTFE tubing, some SPE sorbents, standard bench supplies—means that blank contamination control is as analytically demanding as the detection itself. EPA 537.1 requires that sample volumes of approximately 250 mL be extracted and reconstituted in as little as 1 mL, creating a 250:1 concentration factor that can expose even trace contamination in the sample processing chain. The installation of a delay column between the LC pump and autosampler to prevent instrument background interference is now standard practice.This is precisely where robust, <a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/article/5385">inert nitrogen evaporation </a>systems earn their keep. PFAS workflows that use nitrogen-assisted evaporation to concentrate extracts prior to reconstitution benefit from materials that avoid PTFE and other fluoropolymers that can leach PFAS background—a consideration Organomation addresses in its concentration system designs.The analytical community is also moving beyond targeted PFAS analysis. High-resolution accurate mass (HRAM) spectrometry via Orbitrap technology, paired with non-targeted screening workflows, is gaining traction for the identification of the more than 5,000 currently catalogued PFAS compounds and the novel structures that continue to emerge. LC–MS/MS remains the quantitative gold standard; HRAM provides the discovery layer.<h3>Technological Trends Reshaping the LC–MS/MS Workflow</h3><h4>Artificial Intelligence and Automated Data Interpretation</h4>The datasets generated by modern tandem MS platforms—particularly in omics applications—are too large and too complex for conventional manual review. AI-driven spectral deconvolution, automated peak integration, and AI-assisted hypothesis generation are now embedded in the software suites accompanying leading instruments. <a target="_blank" rel="noopener noreferrer" href="https://www.agilent.com/about/newsroom/presrel/2022/17feb-gp22004.html?srsltid=AfmBOop3warRIaEojXM2YyMML6RXfMIDzE-Wm0h-SiKkkocBeeET2m7u">Agilent’s acquisition of Virtual Control’s ACIES </a>software specifically to automate LC–MS and GC–MS data analysis is emblematic of this direction.For laboratory managers, AI integration means smaller specialized teams can operate high-throughput multi-analyte workflows—but it also means that the front-end sample preparation must be even more reproducible, since algorithmic interpretation amplifies inconsistencies introduced upstream.<h4>Online SPE Coupling and “Chromatography Without Walls”</h4>A significant trend is the integration of solid-phase extraction directly into the HPLC flow path—so-called online SPE–HPLC coupling. Systems that combine extraction and chromatographic separation in a single analytical instrument reduce manual transfer steps, minimize the risk of sample loss or contamination, and dramatically increase throughput. For analytes at very low concentrations, online SPE provides the on-column concentration capability that traditional offline preparation achieves with manual evaporation steps.That said, offline sample preparation—including <a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/article/5392">nitrogen-assisted evaporation</a> under controlled conditions, <a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/products/2407">liquid–liquid extraction</a>, and protein precipitation—retains significant advantages in flexibility, matrix handling, and the ability to process sample volumes that exceed online system capacity. The two approaches are increasingly complementary rather than competitive in well-designed analytical laboratories.<h4>High-Purity Solvents and Reference Materials</h4>As detection limits push further into the femtogram range and regulatory methods demand demonstrable blank performance, the quality of analytical consumables—mobile phase solvents, derivatization reagents, reference standards—has moved from background consideration to front-of-mind. Method validation in regulated environments now routinely requires vendor certification of solvent purity at a level that directly interfaces with instrument performance.<h3>The Ever-Present Need for Sample Evaporation</h3>As LC–MS/MS methods push detection limits lower, the analytical burden shifts increasingly upstream. The quality of the concentration, cleanup, and reconstitution steps preceding injection determines whether the instrument’s theoretical sensitivity is achievable in practice.Several specific developments are particularly relevant:<ul><li>ADC and gene therapy characterization workflows require gentle, low-temperature evaporation that preserves protein structure and prevents aggregation prior to LC–MS/MS. Nitrogen evaporation under controlled conditions is the preferred approach.</li><li>PFAS trace analysis demands evaporation systems constructed from PFAS-inert materials, with rigorous attention to avoiding fluoropolymer surfaces throughout the sample path.</li><li>High-throughput pharmaceutical QC driven by reshoring demands parallel processing capability—multi-position evaporators that maintain consistent flow and temperature across all positions simultaneously.</li><li>Multi-omics and metabolomics workflows generate large sample batches that benefit from automated, walk-away evaporation with programmable endpoints to ensure reproducible final volumes across a cohort.</li></ul>Tandem chromatography–mass spectrometry is not merely growing; it is becoming structurally embedded in the analytical workflows of every major regulated industry. The convergence of biopharma complexity, PFAS regulatory mandates, AI-enabled data analysis, and pharmaceutical reshoring is creating a multi-year demand cycle that will require laboratories to invest not only in instruments, but in the entire sample preparation ecosystem that makes those instruments perform.For analytical chemists, the practical takeaway is this: as mass spectrometry sensitivity continues to advance, the sample preparation step becomes the binding constraint on method performance. Whether you are developing a new PFAS method to meet EPA 1633, characterizing an ADC’s drug-to-antibody ratio, or running a 500-sample metabolomics cohort overnight, the reliability and cleanliness of your concentration and cleanup workflow determines the quality of every data point that follows.&nbsp;

The Continued Rise of Tandem Chromatography–Mass Spectrometry

<h3>A Practical Guide to Getting the Most from Syringe Filters: Tips, Tricks, and Common Pitfalls</h3>Syringe filters play a crucial role in chromatography and many other laboratory applications by removing particulate matter before analysis. Proper sample filtration helps protect analytical instruments, extends column lifetime, and improves data quality. However, selecting an unsuitable filter—or using one incorrectly—can introduce contamination, reduce filtration efficiency, or even compromise analytical results. The following recommendations will help you avoid common mistakes and ensure reliable filtration every time.<img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/KNAUER_Avoiding_Common_Syringe_Filter_Mistakes_3_150e8c1c2b.webp" alt="KNAUER: Avoiding Common Syringe Filter Mistakes" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_KNAUER_Avoiding_Common_Syringe_Filter_Mistakes_3_150e8c1c2b.webp 133w," sizes="100vw" width="1276" height="1496"><h4>1. Improper Filter Handling</h4>Using excessive force during filtration or attempting to reuse disposable syringe filters can damage the membrane and increase the risk of sample contamination.<ul><li>Solution: Apply slow, even pressure throughout the filtration process and always dispose of single-use filters after one use. If the flow rate decreases noticeably, replace the filter instead of applying additional force.</li></ul><h4>2. Selecting an Inappropriate Pore Size</h4>Using the wrong pore size may either fail to remove unwanted particles or cause the membrane to clog prematurely, reducing filtration efficiency.<ul><li>Solution: A 0.45 µm membrane is generally suitable for routine particulate removal, while a 0.22 µm membrane is recommended for finer filtration and bacterial removal. Samples with higher viscosity may require a pre-filter to prevent rapid clogging. It is also important to consider the intended analytical technique. For example, 0.22 µm filters are typically preferred for UHPLC and LC-MS applications, whereas 0.45 µm filters are often sufficient for conventional HPLC analyses.</li></ul><h4>3. Choosing the Wrong Membrane Material</h4>Different membrane materials exhibit different chemical resistance. Using an incompatible membrane may lead to degradation of the filter, sample contamination, or complete filtration failure.<ul><li>Solution: Always verify solvent compatibility before filtration. PTFE membranes are an excellent choice for organic solvents, while PES and nylon membranes are better suited to aqueous samples. If compatibility is uncertain, perform a small-scale trial before filtering valuable samples.</li><li>Pro Tip: For additional guidance, see our blog article "How to Choose the Right Membrane for your Syringe Filter", which provides a detailed comparison of membrane materials and their recommended applications.</li></ul><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/KNAUER_Avoiding_Common_Syringe_Filter_Mistakes_2_e47021fb11.jpg" alt="KNAUER Sepapure® Syringe Filters are available with multiple membrane materials, each identified by a dedicated color code for quick and reliable selection." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_KNAUER_Avoiding_Common_Syringe_Filter_Mistakes_2_e47021fb11.jpg 245w," sizes="100vw" width="1580" height="902"><h4>4. Ignoring Filter Capacity</h4>Using a filter with insufficient capacity can result in rapid membrane blockage, increasing filtration time and reducing laboratory productivity.<ul><li>Solution: When filtering larger sample volumes, choose high-capacity syringe filters or introduce a pre-filtration step. Never force liquid through a clogged membrane, as doing so may damage the filter and negatively affect sample quality.</li></ul><h4>5. Overlooking Sample Adsorption</h4>Certain membrane materials may adsorb analytes of interest, leading to lower recoveries and potentially affecting quantitative results.<ul><li>Solution: For protein samples or other adsorption-sensitive analytes, select membranes with low binding characteristics, such as nylon or glass fiber, whenever appropriate. Performing a small recovery test before routine analysis can help identify potential analyte losses.</li></ul><h4>6. Failing to Optimize Filtration Performance</h4>Slow filtration is more than just an inconvenience—it often indicates that the filtration conditions are not optimal.<ul><li>Solution: If filtration becomes unusually slow, investigate possible causes such as membrane clogging, excessive sample viscosity, or an unnecessarily small pore size. Depending on the sample, dilution or sequential filtration using progressively finer filters may significantly improve performance.</li></ul><h4>Final Thoughts</h4>Selecting the appropriate syringe filter is a straightforward process, but overlooking small details can have a significant impact on analytical performance. Choosing the correct membrane material, pore size, and filtration strategy helps improve laboratory efficiency, protects chromatography systems and columns, and contributes to accurate, reproducible analytical results.With the right syringe filter and proper filtration technique, you can minimize sample preparation problems and maximize confidence in every analysis.<img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/KNAUER_Avoiding_Common_Syringe_Filter_Mistakes_4_983a15e289.jpg" alt="KNAUER: Sepapure® Syringe Filter Selection" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_KNAUER_Avoiding_Common_Syringe_Filter_Mistakes_4_983a15e289.jpg 245w," sizes="100vw" width="1160" height="627">Happy filtering!

Avoiding Common Syringe Filter Mistakes

News from LabRulezLCMS Library - Week 27, 2026

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Everything from the world of analytical chemistry in one place. We connect people in solving their problems. At Labrulez you will find all the necessary information easily, quickly and clearly. Stop searching and start finding.

LabRulez

Organomation, founded in 1959, designs and manufactures high-quality nitrogen evaporators and extraction systems. Known for innovation and durability, their lab instruments are used globally for efficient sample preparation with strong customer support.

Organomation

Based in Berlin, KNAUER is a medium-sized, owner-managed company that has been serving the sciences since 1962. We develop and manufacture scientific instruments of superior quality for liquid chromatography, including: Analytical HPLC/UHPLC, Preparative HPLC, Fast protein liquid chromatography (FPLC), Multi-column chromatography/Simulated moving bed (SMB) chromatography. High pressure dosing, in-line detection systems, and osmometry are additional business areas.

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Agilent Technologies

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Shimadzu Corporation

Waters Corporation

<ul>
<li>Wait what?! Who knew!? There are so many things that a mass spec can do.
</li>
<li>I&#39;ve used it for years, yes this may be true.
</li>
<li>I&#39;ve processed some samples, more than a few.
</li>
<li>But between me and you, I had no idea all the things it could do!
</li>
<li>From sample prep to separation, you just won&#39;t believe…
</li>
<li>All of the tips and tricks we have up our sleeve.
</li>
<li>So, take a few minutes, about 15 should do.
</li>
<li>And learn all the things your mass spec can do.
</li>
</ul>
A triple quad does more than just MRM. In this webinar the objective is to explore the different scan functions of a triple quad and how they can be used to solve complex problems. We will give examples of using the precursor ion and neutral loss scanning for lipid analysis. We will also talk about the unique mixed mode acquisition capability of the Agilent Ultivo Triple Quadrupole mass spectrometer, which allows for the sequential use of different acquisition modes in a single injection. Examples of field applications where mixed mode analysis would be fundamentally valuable will also be discussed.
<blockquote>
Presenter: Julie Cichelli, PhD (Agilent LC/MS applications Scientist, Agilent Technologies, Inc.)
</blockquote>
<blockquote>
Julie Cichelli obtained her Bachelor’s Degree from Villanova University in 2003 with a major in chemistry and a minor in mathematics. She went on to pursue graduate school at the University of Utah, studying under Dr. Zharov, completing her Ph.D. in Organic Chemistry in 2008. Following graduate school in 2009 she accepted a postdoctoral fellowship at the Huntsman Cancer Institute in Salt Lake City researching colon cancer under Dr. Stafforini and Dr. Topham. Subsequently, she joined the Agilent LC/MS applications team in 2010, with a focus in clinical and forensic applications.
</blockquote>
<blockquote>
Presenter: Sheher Banu Mohsin, Ph.D. (Senior Applications Scientist, Agilent Technologies, Inc.)
</blockquote>
<blockquote>
Sheher Mohsin is a senior applications scientist at Agilent Technologies. She received her Ph. D in physical chemistry from the University of Illinois and an MBA from Rockhurst University. She started her career at the US Environmental Protection Agency working on dioxin analysis with high resolution mass spectrometers. She later joined Bayer and worked in the special analysis lab using mass spectrometry to solve problems in synthesis, impurity determination and submission of final product impurity profile to regulatory agencies. Sheher’s current focus is on lipidomics using GC, LC and SFC separations and mass spectrometry. Sheher collaborates with academic and government researchers working on complex problems to come up with innovative, simplified workflows using the latest tools in separation and mass spectrometry.
</blockquote>

Agilent Technologies: Exploring the Different Scan modes of a Triple Quad beyond Just MRMs

Exploring the Different Scan modes of a Triple Quad beyond Just MRMs

In this webinar the objective is to explore the different scan functions of a triple quad and how they can be used to solve complex problems.

The Different Scan modes of a Triple Quad beyond Just MRMs

Identifying fentanyl analogs and other novel psychoactive substances (NPS) in the recreational drug market poses a significant analytical challenge for forensic toxicology laboratories.
In this webinar, two expert speakers will showcase how accurate mass diagnostic fragments of fentanyl and other analogs can be utilized to identify new fentanyl analog biomarkers. They will demonstrate the efficiency and simplicity of SCIEX Molecule Profiler software as a comprehensive and user-friendly platform for identifying novel metabolites, precursors and impurities of emerging drugs.
Attend this webinar to learn about:
<ul>
<li>The challenges associated with the identification of fentanyl analogs and other NPS emerging on the recreational drug market</li>
<li>The use of accurate mass diagnostic fragments for the identification of drug biomarkers</li>
<li>The streamlined process of identifying drug biomarkers using diagnostic fragment screening with SCIEX Molecule Profiler software</li>
<li>The benefits of this approach for the identification of novel metabolites, precursors and impurities of emerging new drugs</li>
</ul>
<blockquote>
Presenter: Dr. Aaron Stella (R&amp;D Scientist, SCIEX)
</blockquote>
<blockquote>
Presenter: Dr. Karl Oetjen (Senior Scientist, Technical Marketing, SCIEX)
</blockquote>

Uncovering Novel Psychoactive Substance Biomarkers Using Diagnostic Fragment Screening

We will showcase how accurate mass diagnostic fragments of fentanyl and other analogs can be utilized to identify new fentanyl analog biomarkers.

NPS Biomarkers Using Diagnostic Fragment Screening

This presentation will focus on our use of a RapidFire 400 High Throughput Mass Spectrometry system coupled to a triple quadrupole mass spectrometer, to build and implement a single platform for the rapid biochemical characterization of multiple biological enzyme targets.
Because of the high sample throughput compared to traditional LC-MS/MS, we can comprehensively characterize the activity of multiple enzymes across a variety of substrates including biologically relevant substrates (e.g. oligonucleosomes) and tailor our Medicinal Chemistry small molecule compound design efforts to target the enzyme activity of interest. Employing a single platform with internal redundancy, enables us to both quickly and confidently develop suites of assays within and across multiple enzyme classes, without many of the limitations present with other technologies.
<blockquote>
Presenter: Patrick J. Bingham (Senior Scientist, Pfizer)
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Agilent Technologies: RapidFire affords extreme versatility in one platform for detecting reaction analytes across a diverse group of enzyme targets

RapidFire affords extreme versatility in one platform for detecting reaction analytes across a diverse group of enzyme targets

This presentation will focus on our use of a RapidFire 400 High Throughput Mass Spectrometry system coupled to a triple quadrupole mass spectrometer.

Reaction analytes across a diverse group of enzyme targets

Data independent acquisition (DIA) allows for suspect screening in complex matrices with retroactive analysis capabilities important to emerging compounds of interest in food safety. However, with increased analyte scope, the review of questionable identifications can create an analysis burden requiring timely review and reinjections.
Here we present a robust DIA methodology with a novel prototype LC/Q-TOF, leveraging a combination of high-resolution, extended dynamic range, stable accurate mass, and isotopic fidelity with coeluting fragmentation ions for identification, analyzed with MH Quantitative Analysis 12.1 and LC Screener Tool.
Over 200 analytes were identified from a spiked broccoli matrix prepared with QuEChERS, resulting in good calibration curve linearity and low area %RSDs. Fused, is the Intelligent Reflex Workflow used to automatically evaluate and reinject samples in which results were flagged as questionable and/or identified, via targeted MS/MS utilizing the available m/z and RT information. Transferring questionable results into confirmed identifications by acquiring targeted data for present compounds. Leveraging the combination of DIA and automatically enabled targeted MS/MS reinjections via Intelligent Reflex allows for broad screening analysis and confident identification confirmation of analytes.
<blockquote>
Presenter: Cathyrin Simmermaker (LC/MS Applications Engineer, Agilent Technologies, Inc.)
</blockquote>
<blockquote>
Cate Simmermaker is an LC/MS Applications Engineer with Agilent Technologies working primarily with high-resolution mass spectrometry in small molecule analysis. Cate has worked in applications including metabolomics, food safety, and forensics on Q-TOF and QQQ platforms. She is currently pursuing her PhD in Chemistry from the University of the Pacific and received a Master’s in Biochemistry from San Francisco State University.
</blockquote>
<blockquote>
Presenter: Christian Klein, PhD (Product Manager LC/Q-TOF, Agilent Technologies, Inc.)
</blockquote>
<blockquote>
With an education in chemistry, Chris Klein received his PhD in 2005 at the Max-Planck-Institute for Biochemistry in Martinsried, Germany, working on membrane proteins and mass spectrometry. After postdoctoral research and three years as a demo chemist and product specialist with Bruker Daltonics, Chris joined the Agilent LC/Q-TOF research and development team in 2010. Since 2018, he has been the LC/Q-TOF and IM-QTOF product manager.
</blockquote>

Agilent Technologies: From Screening to Confirmation: A Comprehensive Food Safety Analysis from Initial DIA Screening to Automatic Reinjection for Suspect Confirmation

From Screening to Confirmation: A Comprehensive Food Safety Analysis from Initial DIA Screening to Automatic Reinjection for Suspect Confirmation

Here we present a robust DIA methodology with a novel prototype LC/Q-TOF.

From Screening to Confirmation: Food Safety Analysis

<h3>Part 1: Overview of Agilent Biopharma</h3>
<blockquote>
Presenter: Mike Knierman (Biopharma Workflow Manager, Agilent Technologies, Inc.)
</blockquote>
<h3>Part 2: Analytical Innovations to Speed-up Antibody Characterization</h3>
The characterization biotherapeutics is essential to assess their safety, quality, and efficacy. In this presentation, we show novel technologies that can address the growing demand for comprehensive and faster characterization of multi-specific antibodies. In the first example, Mobile Affinity Selection Chromatography is used to rapidly assess antibody titer and aggregation levels of crude culture filtrate. We take advantage of this method for unbiased analysis of mispaired chains and aggregates directly from culture supernatants obviating a protein A clean-up step. We show fast aggregate and titer molecular structure quantifications without sample preparation can be achieved with multi-sampler overlapped injections, dramatically improving analysis throughput (~ 2.5 min per sample) without carryover. In the second example, mAb digestion process (&gt; 30 min) is accelerated on the surface of microdroplets (&lt;1 ms) (Anal. Chem., 2021, 93, 3997-4005). We show automated microdroplet reactions under native-pH conditions by combining programmed flow injection with a JetStream ionization source. Intact subunit mass analysis and middle-down characterization of antibody subunits are completed every 2 min. (Anal. Chem., 2023, 95, 3340-3348). Finally, we propose droplet reactions every 2-4 seconds per antibody sample using RapidFire BLAZE mode for ultrafast sample introduction and digestion. We envision, that these analytical innovations will enable comprehensive and faster analysis of biotherapeutics.
<blockquote>
Presenter: Harsha Gunawardena, Ph.D. (Senior Scientist, Mass Spectrometry, Janssen Research &amp; Development, The Janssen Pharmaceutical Companies of Johnson &amp; Johnson)
</blockquote>
<blockquote>
Harsha Gunawardena obtained his Ph.D. in Analytical Chemistry from Purdue University and a Master of Science degree in Analytical Chemistry from University of North Carolina. He was a post-doctoral fellow at the Lineberger comprehensive cancer center, UNC school of medicine where his research focused on refining quantitative platforms for large-scale clinical proteomics and proteogenomic analysis. He was a co-investigator for the NCI clinical proteomics tumor analysis consortium (CPTAC), the ENCODE consortium. His industry career as scientist started at Bayer Health Care providing mass spectrometry and bioanalytical support of plasma-based therapeutics. He later worked at Amgen applying proteomics methods to support biomarker programs and discovery programs in oncology and inflammation. In his current role at Janssen R&amp;D, Johnson &amp; Johnson, he supports discovery early development programs that span several therapeutic modalities.
</blockquote>
<h3>Part 3: Towards the Absolute Quantitation of the Human Proteome: Next Generation of Absolute Protein Quantitation in Biological Matrixes; Higher Multiplexicity While Faster, Robust and Sensitive</h3>
<blockquote>
Presenter: Christoph Borchers, Ph.D. (Professor, Department Oncology, McGill University; CSO, MRM Proteomics)
</blockquote>
<blockquote>
Professor in the Department of Oncology at McGill University, and holds the Segal Chair in Molecular Oncology. His research involves proteomics and metabolomics technologies for clinical diagnostics and structural proteomics. He is also head of a newly established Omics laboratory for personalized medicine and health at Skoltech, Moscow, Russia.
</blockquote>

Accelerating Analysis in the BioPharma Laboratory

Overview of Agilent Biopharma. Analytical Innovations to Speed-up Antibody Characterization. Absolute Protein Quantitation in Biological Matrixes.

First of all, Lakshmanan Deenadayalan will present a sensitive method for the quantification of formoterol – a drug used to treat asthma and COPD – in human plasma.
Then, Eshani Nandita will introduce a sensitive multiplexed quantification of two commonly co-administered mAbs using the ZenoTOF 7600 system with SCIEX OS software.
Finally, Ian Moore will show us how to achieve increased sensitivity for quantification in complex matrices using the SCIEX 7500 system.
At the end of the presentations, you will be able to interact with the speakers and ask any questions.
Attend this webinar to:
<ul>
<li>Meet front-end technology that improves your quantitative mass spectrometry methods</li>
<li>Achieve ultra-low quantification levels for compounds in human plasma using a simple liquid-liquid extraction sample preparation</li>
<li>Reach LLOQs of 0.15 µg/mL for the quantification of monoclonal antibodies in human serum with a QTOF instrument</li>
<li>Explore lower dosage, higher efficacy compounds and improve the efficiency of bioanalysis in negative mode</li>
</ul>
<blockquote>
Presenter: Ian Moore (Senior Technical Product Manager, Nominal Mass SCIEX)
</blockquote>
<blockquote>
Presenter: Lakshmanan Deenadayalan (Application Support Specialist, Indian Innovation Center (IIC) SCIEX)
</blockquote>
<blockquote>
Presenter: Eshani Nandita (Global Biopharma Quant Tech Marketing Lead, SCIEX)
</blockquote>

Pixabay/Gerd Altmann: Inside the Tech Note: The Story Behind the Data

Inside the Tech Note: The Story Behind the Data

In this webinar, hear three LC/MS Tech Note authors present their work and explain the genesis of their research.

This meeting covers the latest trends and techniques to meet current water and environmental testing demands for emerging contaminants and evolving regulations. Our guest speakers from the water industry and environmental research and testing laboratories will share their expertise on their latest work, analytical workflows, and automation.
Join us and discover valuable insights to maximise testing productivity and instrument uptime, simplify workflows and standardise data workup.
<h3>June 27 (Day 1) Emerging contaminants</h3>
This session will explore PFAS, microplastics, untargeted screening, automation and sample preparation.
Attend the whole day or selected presentations only.
<h5>10:00 Online access opens (Streaming begins 09:30 BST/10:30 CEST)</h5>
<h5>10:30 Opening of the event, welcome and introduction</h5>
<ul>
<li>Presenter: Jeff Stubbs (Thermo Fisher Scientific)</li>
</ul>
<h5>10:50 Screening of unknowns using Thermo Scientific Orbitrap MS and Thermo Scientific Compound Discoverer software</h5>
<ul>
<li>Presenter: Janine Elliot (Scottish Environment Protection Agency)</li>
</ul>
<h5>11:20 Biotoxins, seafood safety and mass spectrometry</h5>
<ul>
<li>Presenter: Ben Maskrey (Centre of Environment, Fisheries and Aquaculture Science)</li>
</ul>
<h5>11:50 Determination of microplastics with pyrolysis and GC-MS</h5>
<ul>
<li>Presenter: Dr. Michael Soll (Frontier Lab Europe)</li>
</ul>
<h5>12:20 Break</h5>
<h5>12:40 What combustion ion chromatography sum parameter analysis can do for the analysis of PFAS</h5>
<ul>
<li>Presenter: Dr. Lewis Mark (A1-envirosciences Limited)</li>
</ul>
<h5>13:10 Use of Thermo Scientific Exploris 120 Orbitrap HRAM-LC-MS for the analysis of 47 PFAS compounds in UK waters</h5>
<ul>
<li>Presenter: John Quick (ALS Environmental Ltd.)</li>
</ul>
<h5>13:40 Workshop - new untargeted PFAS analysis workflow capabilities within Compound Discoverer software</h5>
<ul>
<li>Presenter: Frans Schoutsen (Thermo Fisher Scientific)</li>
</ul>
<h5>14:10 Break</h5>
<h5>15:10 Starting your voyage into PFAS? Tools and protocols to succeed</h5>
<ul>
<li>Presenter: Ed George (Thermo Fisher Scientific)</li>
</ul>
<h5>16:10 Workshop - new approaches in performing automation by GC (and LC)</h5>
<ul>
<li>Presenter: Dan Carrier (Thermo Fisher Scientific)</li>
</ul>
<h5>16:30 Closing of the live streaming event</h5>

Thermo Scientific: Water & Environmental Analysis User Meeting - Day 1

Water & Environmental Analysis User Meeting - Day 1

Streaming live from our Demo Centre in Hemel Hempstead | Join the complete event or the presentations of your choice only.

This meeting covers the latest trends and techniques to meet current water and environmental testing demands for emerging contaminants and evolving regulations. Our guest speakers from the water industry and environmental research and testing laboratories will share their expertise on their latest work, analytical workflows, and automation.
Join us and discover valuable insights to maximise testing productivity and instrument uptime, simplify workflows and standardise data workup.
<h3>June 28 (Day 2) – Evolving regulations</h3>
This session will explore disinfection by-products, haloacetic acids, heavy metals, volatiles/semi-volatiles analysis.
Attend the whole day or selected presentations only.
<h5>10:00 Online access opens (Streaming begins 09:30 BST/10:30 CEST)</h5>
<h5>10:30 Opening of the event, welcome and introduction</h5>
<ul>
<li>Presenter: Ed George (Thermo Fisher Scientific)</li>
</ul>
<h5>10:50 Beyond compliance - use of the Orbitrap Exploris 120 HRAM-LC-MS for the monitoring of catchments</h5>
<ul>
<li>Presenter: Mark Collins (Northern Ireland Water)</li>
</ul>
<h5>11:20 Utilizing IC-MS/MS for the analysis of halo acetic acids and disinfection by products in drinking water</h5>
<ul>
<li>Presenter: Chris Pegg (Anglian Water)</li>
</ul>
<h5>11:50 GC-MS analysis of environmental samples using automated sample preparation</h5>
<ul>
<li>Presenter: John Quick (ALS Environmental Ltd.)</li>
</ul>
<h5>12:20 Break</h5>
<h5>12:40 Development of an ion chromatography method with EC and MS detection for the analysis of bromide and the disinfection by products bromate, chlorate and chlorite</h5>
<ul>
<li>Presenter: Joseph Scott (Affinity Water)</li>
</ul>
<h5>13:10 High throughput nutrients analysis - developing methodologies for environmental and wastewater matrices</h5>
<ul>
<li>Presenter: Red Godwin (Thermo Fisher Scientific)</li>
</ul>
<h5>13:40 Performing the analysis of trace metals in environmental samples via ICP and ICP-MS</h5>
<ul>
<li>Presenter: Trudy McMurray (Northern Ireland Environment Agency)</li>
</ul>
<h5>14:15 Closing of the live streaming event</h5>

Thermo Scientific: Water & Environmental Analysis User Meeting - Day 2

Water & Environmental Analysis User Meeting - Day 2

Expansion of the MRM Toxicology Screening Methodology for Use With Waters Xevo TQ-S micro Tandem mass spectrometry has gained popularity over the years owing to its analytical sensitivity and selectivity, especially when used in MRM mode.
MRM can be used for quantitative analysis as well as targeted qualitative screening. Broad screening techniques that can detect a wide variety of toxicants in complex biological matrices are very much in demand in forensic toxicology laboratories.
At Waters, we have provided and supported, ready-to-implement MRM based toxicology screening solutions for almost two decades. 
This session will detail the further development of the popular Xevo TQ-S micro method and the expansion of the MRM Toxicology Screening Methodology which includes the addition of novel psychoactive substances (NPS) and other new drug classes.
Learning objectives – what you will learn:   
<ul>
<li>Expansion of the MRM nominal mass screening method</li>
<li>How the samples were prepared and data utilized</li>
<li>Total number of substances added and their drug classes</li>
</ul>
<blockquote>
Presenter: Nayan. S. Mistry (Senior Application Scientist, Forensics, Research &amp; Development, Waters Corporation, Waters Corporation)
</blockquote>
<blockquote>
Nayan Mistry is a Senior Application Scientist at Waters Corporation. Nayan joined Waters in 2009 and is part of a global team dedicated to performing research and development of applications and analytical solutions for Forensic Toxicology. 
</blockquote>
<blockquote>
Nayan’s key focus is on toxicology screening and he has expertise with both nominal mass and accurate mass-based screening using UNIFI and Waters_Connect application software. Nayan plays a key role in the development of these solutions and in the expansion of associated libraries. Currently Nayan is playing a key role in the development and support of the recently launched Xevo G3 QTof mass spectrometer in addition to other responsibilities for the nominal mass applications. His favourite aspect of the role is customer support - Nayan is greatly involved in global on-site customer support and training and has presented his work at conferences worldwide.
</blockquote>

Waters Corporation: Expanded Screening with a Nominal Mass Tandem Quadrupole System

Expanded Screening with a Nominal Mass Tandem Quadrupole System

This session will detail the further development of the popular Xevo TQ-S micro method and the expansion of the MRM Toxicology Screening Methodology.

Screening with a Nominal Mass Tandem Quadrupole MS

This webinar introduces an analytical method that utilizes ion-exchange chromatography to separate and analyze oligonucleotides of different lengths.
The primary focus is on eliminating impurities that are produced during the synthesis process. The method incorporates an inert ultra-high-performance liquid chromatography system, liquid chromatography-tandem mass spectrometry, and automated data analysis software. These components work together to facilitate efficient oligonucleotide development and quality control. The results emphasize the challenges associated with metal-based methods and propose a metal-free approach for precise quantification. Additionally, a high-resolution ion-pair reversed-phase LC-MS system is presented for accurate separation and mass analysis of oligonucleotides. The developed method also offers sensitive, precise, and reproducible results, making it an invaluable tool for various scientific fields, including therapeutic oligonucleotide-based drug development and quality control.
<blockquote>
Presenter: Mridul Mandal, Ph.D.
</blockquote>

Shimadzu: Oligonucleotide Analysis by LC and LC and LC-MS

Oligonucleotide Analysis by LC and LC and LC-MS

Analytical method that utilizes ion-exchange chromatography to separate and analyze oligonucleotides of different lengths.

LCMS webinars focusing on LC/MS/MS - page 67