<p><strong>Professor Michaela Rumlová, head of a research group of the National Institute of Virology and Bacteriology at UCT Prague (and Vice-Rector for doctoral studies), holder of a licensed patent &nbsp;for antiretroviral testing assays, and recipient of the Czech Science Foundation’s President’s Award for HIV research, has also become the principal investigator of the bilateral INTER-EXCELLENCE II project “</strong><i><strong>Cellular factors regulating the export and packaging of unspliced ​​retroviral RNA</strong></i><strong>”, starting this year. In this interview, she explains how retroviruses such as HIV “hack” the cell in order to export their own genome from the nucleus into the cytoplasm, and then to the site where it is specifically packaged into nascent viral particles, a key step in viral replication. Her team is collaborating with the NIH (National Institutes of Health) and Rockefeller University to elucidate mechanisms that may help reveal new vulnerable points in the retrovirus replication cycle while simultaneously clarifying related cellular processes.</strong></p><h5><strong>Could you describe your project and its main objectives?</strong></h5><p>Retroviruses, such as HIV, are viruses that carry genetic information in the form of RNA; however, after entering a cell, they are able to reverse transcribe it into DNA and integrate it into the host genome. In order to replicate, they must transport their genetic information—in the form of unspliced RNA—from the nucleus into the cytosol. There, it serves both as a template for the production of viral proteins and as the genome of new viral particles. This is highly unusual, as the cell very carefully controls which RNA molecules are allowed to leave the nucleus. Normal protein-coding cellular RNAs, known as messenger RNAs (mRNAs), must undergo splicing—a processing step in which certain segments are removed—before being exported. When this process fails, mRNA that retains these segments is held back in the nucleus as part of quality control and subsequently degraded. Retroviruses have managed to outwit this cellular control machinery, allowing their unspliced RNA to pass into the cytoplasm.</p><p>Our project focuses on identifying the cellular proteins that assist retroviruses in this step and on the mechanism by which protein complexes bound to viral RNA are remodelled during RNA transport. The goal is to understand how the virus uses cellular mechanisms to its advantage and how this pathway is linked to the subsequent fate of the RNA, i.e. to its translation and packaging into new viral particles.</p><h5><strong>Why is this export of unspliced ​​RNA so important for retroviral replication?</strong></h5><p>Because this RNA has a dual role for the virus. On the one hand, it serves as a template for synthesizing viral proteins in the cytoplasm; on the other, it represents the genetic material itself, which must be packaged into new viral particles. The virus must package its RNA genome in its unspliced form, as only this RNA contains essential sequences without which further viral replication would be impossible.</p><p><strong>Simply put: </strong>the virus needs to get a complete copy of its genetic instructions out of the nucleus. Once the viral RNA is in the cytoplasm, it can be used as a template for synthesizing viral proteins; and a portion of these RNA molecules is then selected and packaged into a new particle as a genome. We are interested in how the virus forces the cell to allow viral RNA that is, from the cell’s perspective, “unfinished,” that is unspliced, to leave the nucleus. We are also investigating how the cell and the virus together - albeit unintentionally from the cell’s perspective - determine which RNA molecule will be translated into proteins and which will be packaged as the genome. This is one of the key, yet still not fully understood, steps of retroviral replication.</p><h5><strong>What do you think it was about your project that impressed the Ministry of Education, Youth and Sports’ jury the most?</strong></h5><p>I believe the decisive factor was a combination of several elements. First, the reviewers were primarily interested in the scientific problem itself. We are investigating a mechanism that is essential for retroviruses, but still not sufficiently understood, which may also have broader implications for the regulation of cellular processes. In addition, the quality of the team, our strong collaboration with the<strong> NIH </strong>and <strong>Rockefeller University</strong>, and the use of modern methods that will allow us to track viral RNA in great detail also played a role. It was also important that the project has clear objectives and a realistic plan.</p><h5><strong>How will this research contribute to the development of new treatments for retroviral infections?</strong></h5><p>It is important to say that this is primarily basic research. Rather than developing a new drug directly, we are trying to understand, at the molecular level, the step that the virus cannot do without. Only when we fully understand the mechanism of these cellular processes and identify the essential factors for the export of unspliced ​​RNA and how they function, will we be able to better determine which interactions or mechanisms represent potentially vulnerable points. Similar mechanisms associated with the export of unspliced ​​mRNA also appear in some diseases, such as cancer, so I &nbsp;see the benefit of our research mainly in that it will refine our understanding of these pathological processes in the cell, and retroviruses help us do so. Therefore, our work on retroviruses not only shows us how the virus works, but also how the cell itself works.</p><h5><strong>What responsibilities does your UCT Prague team have? How does the collaboration with your American partners work?</strong></h5><p>At UCT Prague, we are conducting the project’s primary experimental work. Our research involves cell cultures, viral models, and various biochemical techniques ranging from immunochemistry to microscopy that allow us to track viral RNA and identify its cellular partners. In collaboration with colleagues from <strong>NIH, Frederick</strong>, we are developing single-particle tracking methods to observe viral RNA movement in living cells across both space and time. Furthermore, we are working with colleagues from <strong>Rockefeller University to employ iCLIP </strong>methods for the precise mapping of protein-binding sites on RNA. Both collaborations are valuable to us, because they are not just about data sharing, but also about the transfer of expertise and detailed know-how.</p><h5><strong>Who else is collaborating on the project?</strong></h5><p>At UCT Prague, we collaborate closely with the Department of Biotechnology and the Department of Biochemistry and Microbiology. Externally, we work with colleagues from the Institute of<strong> Organic Chemistry and Biochemistry of the CAS (IOCB Prague)</strong> and the <strong>Central European Institute of Technology in Brno (CEITEC)</strong>. Our research naturally necessitates the integration of diverse fields of expertise; it requires combining virology, cell biology, and biochemistry with advanced imaging and modern approaches to study RNA-protein interactions. This is exactly what makes our project capable of addressing complex questions that a single laboratory cannot tackle on its own.</p><h5><strong>Are there plans to continue the project? Have you brought on new collaborators, and are you looking to welcome new colleagues to the team?</strong></h5><p>Yes, definitely. In basic research, it often happens that one answered question opens up several more. High-quality, properly interpreted data provide a foundation you can build on — and that is what moves scientists forward. It is already clear that the topic of retroviral RNA export and packaging has broader implications and may reveal more general principles of how RNA functions within the cell. We also hope the project will be attractive to students, as it connects classical molecular biology, biochemistry, and virology with cutting-edge biotechnological methods. Students are already involved in the project as part of their bachelor’s and master’s theses.</p><h5><strong>What was the personal turning point that motivated you to pursue a long-term investigation into retroviral particles?</strong></h5><p>I first encountered retroviruses in the early 1990s during my diploma thesis, when I was working at IOCB Prague on HIV-1 protease inhibitors. At that time, AIDS could not yet be effectively treated, and retrovirus research had a completely different dimension. From the very beginning, I was fascinated by how such a relatively small and simple virus can outsmart evolutionarily highly optimized cellular processes and turn them to its own advantage. And that has never stopped fascinating me. From a scientific point of view, retroviruses are remarkable; just consider one of their enzymes, reverse transcriptase, without which no diagnostic or research laboratory in the world could function today. Without it, it would be impossible to diagnose RNA viruses like SARS-CoV-2, influenza, or HIV using RT-PCR tests, or, for example to produce recombinant human proteins in bacterial cells.</p>

Tracking unspliced RNA: Professor Rumlová studies how retroviruses “hack” cells

<p><strong>High-performance liquid chromatography (HPLC) remains one of the most widely used analytical techniques for separating, identifying, and quantifying compounds in complex mixtures. Successful HPLC method development depends not only on selecting the correct column and optimizing the mobile phase composition, but also on choosing the appropriate detector. Even a perfectly optimized chromatographic separation provides little value if the detection system cannot properly capture and interpret the analytes of interest.</strong></p><p>In many ways, the detector acts as the <i><strong>“vision system</strong></i>” of the chromatograph. It converts separated compounds into measurable electronic signals, allowing analysts to identify components and determine their concentrations. The choice of detector therefore has a major impact on sensitivity, selectivity, robustness, and the overall quality of analytical data.</p><p>This article provides an overview of the most common HPLC detection techniques, discusses their strengths and limitations, and outlines the most important criteria to consider when selecting a detector for a specific analytical task.</p><h3><strong>HPLC Detection Technologies</strong></h3><p>HPLC detectors are typically divided into two major categories:</p><ol><li>Specific detectors (solute-property detectors)</li><li>Bulk property detectors</li></ol><p>Specific detectors respond to a particular chemical or physical property of the analyte. Ideally, their signal is largely unaffected by changes in mobile phase composition. Common examples include UV/Vis detectors, fluorescence detectors, and mass spectrometers.</p><p>Bulk property detectors, on the other hand, measure differences between the pure mobile phase and the mobile phase containing analytes. These detectors are considered more universal because they respond to properties shared by many compounds. Refractive index detectors are among the best-known examples, alongside conductivity and light-scattering detectors.</p><p>Both detector categories have important advantages depending on the application, sample type, and analytical goals.</p><h3><strong>Common Detector Types Used in HPLC</strong></h3><p>A wide variety of detectors are available for HPLC systems, ranging from highly universal devices to extremely selective and sensitive technologies.</p><p>Common detector options include:</p><ul><li>UV/Vis detectors</li><li>Diode array detectors (DAD/PDA)</li><li>Fluorescence detectors</li><li>Refractive index detectors (RID)</li><li>Conductivity detectors</li><li>Evaporative light scattering detectors (ELSD)</li><li>Charged aerosol detectors (CAD)</li><li>Mass spectrometry detectors (MS)</li></ul><p>Each technology offers a different balance of sensitivity, selectivity, robustness, operational complexity, and cost.</p><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/KNAUER_Detector_overview_with_characteristics_Graphic_by_KNAUER_84b0dd30b1.jpg" alt="KNAUER: Detector overview with characteristics. Graphic by KNAUER." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_KNAUER_Detector_overview_with_characteristics_Graphic_by_KNAUER_84b0dd30b1.jpg 245w," sizes="100vw" width="1293" height="596"></p><h3><strong>Selecting the Right HPLC Detector</strong></h3><p>There is no universally ideal detector for all HPLC applications. The best choice depends on several factors, including analyte chemistry, matrix complexity, required detection limits, and instrument compatibility.</p><p>Careful detector selection is essential for obtaining reliable and meaningful analytical results.</p><p>The following considerations can help guide the selection process.</p><h4><strong>1. Chemical and Physical Properties of the Analytes</strong></h4><p>The chemical nature of the compounds being analyzed is one of the most important selection criteria.</p><p><strong>Questions to consider include:</strong></p><ul><li>Do the analytes contain chromophores?</li><li>Are the compounds naturally fluorescent?</li><li>What is their polarity?</li><li>What is their molecular weight?</li><li>Are they volatile or non-volatile?</li></ul><p>Compounds containing UV-active groups are often well suited for UV/Vis detection, while fluorescent molecules can benefit from the high sensitivity of fluorescence detectors. Non-UV-active compounds may require universal detection approaches such as CAD, ELSD, or mass spectrometry.</p><h4><strong>2. Required Sensitivity</strong></h4><p>Detector sensitivity determines the minimum concentration that can be reliably detected and quantified.</p><p>Routine quality control analyses often rely on UV/Vis detectors because they provide sufficient sensitivity for many applications while remaining relatively simple and cost-effective. However, trace-level analysis or highly complex matrices may require more advanced technologies such as:</p><ul><li>Fluorescence detection</li><li>Charged aerosol detection</li><li>Mass spectrometry</li></ul><p>These techniques offer significantly lower detection limits and greater analytical specificity.</p><h4><strong>3. Detector Linearity</strong></h4><p>Linearity describes how well detector response correlates with analyte concentration across a defined range.</p><p>A detector with strong linearity ensures accurate quantification and simplifies calibration procedures. Poor linearity can introduce quantification errors, especially when analyzing samples across broad concentration ranges.</p><h4><strong>4. Dynamic Range</strong></h4><p>Dynamic range refers to the concentration interval over which the detector can reliably measure analytes.</p><p>Applications involving samples with highly variable concentrations require detectors capable of maintaining accurate response over a broad range. A wide dynamic range reduces the need for repeated dilutions or multiple analytical runs.</p><h4><strong>5. Selectivity</strong></h4><p>Selectivity defines the detector’s ability to distinguish target analytes from other matrix components.</p><p>This factor becomes particularly important for complex samples containing many interfering substances. Highly selective detectors reduce the risk of overlapping signals and false positives.</p><p>Mass spectrometry is often advantageous when dealing with unknown compounds or highly complicated sample matrices because it provides structural information in addition to quantitative data.</p><h4><strong>6. Budget Considerations</strong></h4><p>Detector cost is another practical factor that laboratories must evaluate.</p><p>UV/Vis and refractive index detectors are generally among the more economical choices, making them attractive for routine analyses and standard applications.</p><p>More sophisticated technologies such as fluorescence detection, CAD, or mass spectrometry typically require:</p><ul><li>Higher initial investment</li><li>Additional maintenance</li><li>More complex infrastructure</li><li>Greater operational expertise</li></ul><p>However, the overall value of the detector should always be considered in relation to analytical performance requirements and long-term application needs.</p><h4><strong>7. Instrument and Mobile Phase Compatibility</strong></h4><p>The selected detector must be compatible with the operational conditions of the HPLC system.</p><p>Important parameters include:</p><ul><li>Flow rate range</li><li>Pressure limitations</li><li>Mobile phase composition</li><li>Solvent compatibility</li></ul><p>Some detectors are highly sensitive to mobile phase composition or gradient changes, while others tolerate a broader range of operating conditions.</p><p>Ensuring proper compatibility helps prevent baseline instability, signal interference, and reduced detector performance.</p><h4><strong>8. Destructive vs. Non-Destructive Detection</strong></h4><p>Another important consideration is whether the analytical workflow involves only detection or also preparative purification.</p><p>Non-destructive detectors allow collected fractions to remain intact after detection, which is highly beneficial for preparative chromatography and purification workflows.</p><p>Destructive detectors, by contrast, consume or alter the sample during measurement and are therefore more suitable for analytical applications where sample recovery is not required.</p><h4><strong>9. Reliability and Ease of Operation</strong></h4><p>Practical usability is often just as important as analytical performance.</p><p>Some detectors are designed for straightforward plug-and-play operation, while others require:</p><ul><li>Frequent calibration</li><li>Specialized maintenance</li><li>Dedicated gas supplies</li><li>Vacuum systems</li><li>Advanced operator expertise</li></ul><p>Laboratories should evaluate the available technical support, user experience, and maintenance capabilities before implementing more sophisticated detection technologies.</p><p>Reliable operation and manageable maintenance requirements can significantly improve laboratory productivity and reduce downtime.</p><p>Choosing the correct HPLC detector starts with understanding both the sample and the analytical objective. Rather than selecting a detector simply because it is available, laboratories should ensure that the technology aligns with the chemistry of the analytes, required performance characteristics, and workflow demands.</p><p>In many cases, combining multiple detectors within a single HPLC system can provide even greater analytical insight by delivering complementary information from the same chromatographic separation.</p><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/KNAUER_Flow_scheme_for_detector_selection_Graphic_by_KNAUER_486004045f.jpg" alt="KNAUER: Flow scheme for detector selection. Graphic by KNAUER." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_KNAUER_Flow_scheme_for_detector_selection_Graphic_by_KNAUER_486004045f.jpg 245w," sizes="100vw" width="1316" height="808"></p><p>Interested in learning more? Future articles will explore individual HPLC detection techniques in greater detail, including their operating principles, application areas, and practical advantages for different analytical workflows.</p>

A comprehensive guide for selecting the appropriate HPLC detector

News from LabRulezLCMS Library - Week 20, 2026

Article

Product

Application

Scientists

Science and research

The University of Chemistry and Technology, Prague is a natural centre of first-rate study and research in the area of chemistry in Czechia and is one of the country's largest educational and research institutions focused on technical chemistry, chemical and biochemical technologies, material and chemical engineering, food chemistry, and environmental studies.

UCT Prague

Everything from the world of analytical chemistry in one place. We connect people in solving their problems. At Labrulez you will find all the necessary information easily, quickly and clearly. Stop searching and start finding.

LabRulez

Based in Berlin, KNAUER is a medium-sized, owner-managed company that has been serving the sciences since 1962. We develop and manufacture scientific instruments of superior quality for liquid chromatography, including: Analytical HPLC/UHPLC, Preparative HPLC, Fast protein liquid chromatography (FPLC), Multi-column chromatography/Simulated moving bed (SMB) chromatography. High pressure dosing, in-line detection systems, and osmometry are additional business areas.

KNAUER

Metrohm

Thermo Fisher Scientific

<p><strong>Your chromatography is only as good as your column: In a free webinar on November 17, 2022, Dr. Vincent Diederich, Product Manager IC Columns at Metrohm International Headquarters, will present you the next generation of Metrohm separation columns for anion chromatography.</strong></p>
<p>New material and innovative design of the stationary phase are behind the outstanding stability, capacity, and resolution of the Metrosep A Supp 19 columns, which will be demonstrated by Dr. Diederich with application examples from the water, waste water, food, and power plant sectors. </p>
<p>Join us on November 17, 2022, at 3:00 p.m. CET and learn how you can take your anion chromatography to the next level.</p>
<h5>Topics of this webinar:</h5>
<ul>
<li>Robust anion analysis of samples with high matrix loads (e.g., carbonate in drinking water, separation of nitrite from chloride in sea and wastewater)</li>
<li>High-res separation of early eluting organic acids (e.g., acetate, formate, glycolate, and others in cooling waters)</li>
<li>Separation of other organic acids (e.g.,  lactate, malate, tartrate, oxalate, etc.) in foods and beverages</li>
</ul>
<blockquote>
<p><strong>Presenter:</strong> Vincent Diederich, PhD (Product Manager IC Columns, Metrohm International Headquarters)</p>
</blockquote>
<blockquote>
<p>Vincent Diederich has been working for more than seven years in the fields of column and method development for ion chromatography at Metrohm International Headquarters. Vincent obtained his PhD in Chemical Engineering at ETH Zurich in Switzerland where he gaind a deep understanding of polymer reaction engineering.</p>
</blockquote>


Metrohm: Redefining anion analysis with a new generation of separation columns

Redefining anion analysis with a new generation of separation columns

New material and innovative design of the stationary phase are behind the outstanding stability, capacity, and resolution of the Metrosep A Supp 19 columns.

Anion analysis with a new generation of separation columns

<p><strong>How to determine AOX and AOF by Combustion Elemental Analysis and Combustion Ion Chromatography?</strong></p>
<p>As the presence of organically bound halogens (AOX) and organically bound fluorine (AOF) in the environment is coming under scrutiny in many countries, there is a need for rapid screening of these parameters in environmental samples. </p>
<p>In a joint webinar by Analytik Jena and Metrohm, Christian Koch (Analytik Jena) and Theresa Steurer (Metrohm) present Combustion Elemental Analysis and Combustion Ion Chromatography (CIC) as a viable solution to determine the sum parameters AOX and AOF.<br/>In contrast to other techniques CIC can determine the individual sum parameters of the individual halogens, e.g., AOF, which is a screening parameter for per-and polyfluorinated alkyl substances (PFAS). Thus, CIC can provide a fast and accurate picture of the overall contamination levels before performing targeted analysis to identify individual PFAS.</p>
<p>As sample preparation is a crucial step in both techniques, this webinar puts a special focus on procedures making sure that only the organic halogen compounds are measured and any interfering compounds are removed prior to analysis (see ISO 9562; EPA 1650C, and notably recently published DIN 38409-59).</p>
<p>This webinar is intended for lab managers and analytical chemists looking for solutions to the challenges posed by AOX and PFAS monitoring in wastewater, surface water, and other water matrices.</p>
<p>Participants in this webinar will learn about:</p>
<ul>
<li><strong>the regulatory background</strong></li>
<li><strong>the challenges of sample preparation</strong></li>
<li><strong>combustion / pyrohydrolysis as an automated sample preparation technique</strong></li>
<li><strong>the analytical procedures for analysis of AOX by Combustion Elemental Analysis</strong></li>
<li><strong>the analytical procedures for analysis of AOF by Combustion Ion Chromatography</strong></li>
</ul>


Metrohm: Monitoring PFAS and other organically bound halogens in water and wastewater

Monitoring PFAS and other organically bound halogens in water and wastewater

How to determine AOX and AOF by Combustion Elemental Analysis and Combustion Ion Chromatography

PFAS and organically bound halogens in water and wastewater

<p><strong>Ion Chromatography (IC) is an established and useful analytical tool for pharmaceutical applications. Many times, IC is faster, easier to implement, and offers significant advantages over traditional wet chemistry methods. In fact, the United States Pharmacopeia has modernized a substantial number of monographs to stipulate IC methods for numerous pharmaceutical assays of APIs and analysis of impurities and excipients.</strong></p>
<p>In this webinar, you will hear about recently validated IC methods for various assays and their associated impurities and challenges associated with some magnesium-based OTC products.</p>
<p>We will also review the basics of IC and various sample preparation techniques to overcome difficult sample matrices. A live Q&amp;A session will be hosted at the end of the webinar to address any questions you may have.<br/>Key learning points of this webinar:</p>
<ul>
<li>Understand newly validated IC methods for pharmaceutical applications.</li>
<li>Learn how ion chromatography can be used for fast and reliable salt identification, assay, and impurities. </li>
<li>Learn what strategies are available for automation of sample preparation to deal with difficult sample matrices.</li>
</ul>
<blockquote>
<p><strong>Presenter:</strong> Linx Waclaski (Product Manager Ion Chromatography, Metrohm USA)</p>
</blockquote>
<blockquote>
<p>Linx Waclaski is the Product Manager for Ion Chromatography at Metrohm USA in Riverview, Florida. He has years of experience in multiple chromatographic techniques, including ion chromatography, gas chromatography, liquid chromatography, and mass spectrometry, with extensive experience in R&amp;D and applications development. His chromatography applications experience extends across environmental, food safety, and life sciences industries. He has an M.S. in Forensic Science from Duquesne University.</p>
</blockquote>
<blockquote>
<p><strong>Presenter:</strong> Shibu Paul (Product Specialist Ion Chromatography, Metrohm USA)</p>
</blockquote>
<blockquote>
<p>Shibu Paul, Product Specialist for Ion Chromatography at Metrohm USA, has more than 30 years of experience in the food, environmental and instrumentation industries, working in positions from bench chemist to laboratory director. He has a Ph.D. in Environmental Chemistry from Cochin University of Science &amp; Technology, India and an M.B.A. from the University of South Florida.</p>
</blockquote>


Metrohm: Ion Chromatography for Pharmaceutical Applications

Ion Chromatography for Pharmaceutical Applications

Recently validated IC methods for various assays and their associated impurities and challenges associated with some magnesium-based OTC products.

<p><strong>We have an exciting virtual three-day agenda with great speakers from industry experts, workshops, and poster sessions.</strong></p>
<p>Come and listen to industry experts to discover IC in the world of</p>
<ul>
<li>Glycan research</li>
<li>Pesticides</li>
<li>Metabolomics </li>
<li>Pharmaceutical analysis</li>
<li>Industrial processes</li>
<li>Geochemistry</li>
<li>Food and beverage</li>
</ul>
<p><strong>Discover tools, tips, and tricks to get the most out of your IC analysis in our IC workshops.</strong></p>
<p>Registration and the agenda will be available soon - Watch this space.</p>


Thermo Scientific: Global IC Symposium 2023

Global IC Symposium 2023 - Day 1

We have an exciting virtual three-day agenda with great speakers from industry experts, workshops, and poster sessions.

Thermo Scientific Global IC Symposium 2023

<p><strong>Chromium content and impurity analysis of plating baths using ion chromatography provide a highly accurate and easily automated solution compared with traditional wet chemistry methods. Quality control of these and similar plating bath sample types is essential for dialing in bath performance.</strong></p>
<p>Attend this session to learn how The Armoloy Corporation recently replaced tedious, manual analysis of their chromium baths with IC to monitor chromium and ionic contaminants. You’ll also learn how to combine this knowledge with the fundamentals of IC so that you can apply this to other sample types. Take part in our live Q&amp;A after the presentation to get your specific questions answered by our experts. </p>
<h5><strong>Who should attend?</strong></h5>
<ul>
<li>Analysts, technicians, chemists who want to learn how IC can simplify their analysis, eliminating more cumbersome techniques</li>
<li>Quality lab managers considering implementing IC into their lab</li>
</ul>
<h5><strong>Key learning points</strong></h5>
<ul>
<li><strong>Learn how IC can be used to simplify multiparameter analyses and eliminate tedious wet chemistry methods</strong></li>
<li><strong>Understand how IC can be used as an important tool for quality control in industry</strong></li>
<li><strong>Learn the basics of IC from a theoretical and equipment standpoint</strong></li>
</ul>
<blockquote>
<p><strong>Presenter:</strong> Kris Johnson (Chemist, Armoloy)</p>
</blockquote>
<blockquote>
<p>Kris Johnson is a chemist at Armoloy where he has been for four years and recently completed his CEF. He handles the chemical and metallurgical testing and heads R&amp;D, among other things. Kris graduated from the chemistry program at Northern Illinois University where he was also a researcher and was recognized in three different published articles. He uses his knowledge in the plating industry to participate in the Aerospace Materials Specifications B Committee to review and update several of these specifications used by numerous laboratories worldwide.</p>
</blockquote>
<blockquote>
<p><strong>Presenter:</strong> Bikash Chouhan (Applications Specialist, Metrohm USA)</p>
</blockquote>
<blockquote>
<p>Bikash Chouhan is an Applications Specialist for Ion Chromatography at Metrohm USA. He completed his PhD in Analytical Chemistry from the University of Texas at Arlington (UTA). His area of research during his PhD was in Capillary Scale Ion Chromatography. He has worked as a Post Doc at the Department of Chemistry and Biochemistry, UTA and as a Senior Scientist in IC at Eurofins, before joining Metrohm USA. He is currently involved in expanding IC applications to more unique and challenging sample matrices.</p>
</blockquote>


Metrohm: Analysis of Plating Baths with Ion Chromatography

Analysis of Plating Baths with Ion Chromatography

Learn how The Armoloy Corporation recently replaced tedious, manual analysis of their chromium baths with IC to monitor chromium and ionic contaminants.

<p><strong>This year we invite you to ignite your mind to Be Curious about what Ion chromatography (IC) can do with its versatility in many detections across the IC application spectrum to improve your workflows and solve challenges.</strong></p>
<p>During this three day virtual event, learn from a panel of experts how the latest IC applications are helping colleagues achieve breakthroughs, and walk away with valuable information on how advancements in IC can increase laboratory analytical capabilities. </p>
<p>Although each day is convenient for a specific region, you can attend any session on any day. All sessions and workshops will be available for on-demand viewing after the event concludes.</p>
<h3><strong>Day 1 Agenda | April 18 | North and South America</strong></h3>
<p>Discover IC column development, and where it’s headed with mass spectrometry, as they can be an integral detection for carbohydrate profiling and environmental metabolites characterization. Learn how standalone IC can solve industrial samples and degradation products with comprehensive IC information, all at once.</p>
<h5><strong>11:30 EDT: Opening Plenary: The Past, Present, and Future of IC Column Development</strong></h5>
<ul>
<li>Alexandra Zatirakha (Thermo Fisher Scientific, IC/SP R&amp;D, Sunnyvale)</li>
</ul>
<h5><strong>12:30 EDT: Characterizing Carbohydrate Degradation Products from Bacteroides Thetaiotaomicron to Understand Bacterial Dextran Utilization in the Gut</strong></h5>
<ul>
<li>Neil Rumachik (Thermo Fisher Scientific, IC/SP R&amp;D, Sunnyvale)</li>
<li>Fiona Cuskin (Newcastle University, UK)</li>
</ul>
<h5><strong>1:30 PM EDT: Toward Comprehensive Chemical Characterization of Metabolomic Using Orbitrap Fusion Tribrid Mass Spectrometer Coupled with Ion and Liquid Chromatography Techniques: Dissolved Organic Matter as an Example</strong></h5>
<ul>
<li>Hussain Abdulla (Department of Physical and Environmental Sciences, Texas A&amp;M University-Corpus Christi)</li>
</ul>
<h5><strong>2:30 PM EDT: The Use of Ion Chromatography in the Monitoring of Degradation Products from the Electrochemical Treatment Of Organic Compounds</strong></h5>
<ul>
<li>Emily Tossi (Federal University of Rio Grande do Norte)</li>
</ul>
<h5><strong>3:30 PM EDT: Ion Chromatography as a Tool for Industrial Problem Solving</strong></h5>
<ul>
<li>Isaac Negrete and Stephen Fosdick (Dow)</li>
</ul>
<h5><strong>4:30 PM EDT: Workshop: Everything, Everywhere, All at Once IC!</strong></h5>
<ul>
<li>Kelly Femrite (IC/SP Product Support, Sunnyvale)</li>
<li>Carl Fisher (IC/SP Product Marketing, Sunnyvale)</li>
<li>Criss Corchard-Keeler (Service Marketing Manager, CMD/MOL Thermo Fisher Scientific)</li>
</ul>


Thermo Fisher Scientific: IC Symposium 2023

IC Symposium 2023 - Day 1

Join us for the 2023 Global IC Symposium! North and South America

<p><strong>This year we invite you to ignite your mind to Be Curious about what Ion chromatography (IC) can do with its versatility in many detections across the IC application spectrum to improve your workflows and solve challenges.</strong></p>
<p>During this three day virtual event, learn from a panel of experts how the latest IC applications are helping colleagues achieve breakthroughs, and walk away with valuable information on how advancements in IC can increase laboratory analytical capabilities. </p>
<p>Although each day is convenient for a specific region, you can attend any session on any day. All sessions and workshops will be available for on-demand viewing after the event concludes.</p>
<h3><strong>Day 2 Agenda | April 19 | Europe and Africa</strong></h3>
<p>Untap the versatility of ion chromatography with challenging analytes such as chlorate, sulfite, cationic polar pesticides, and glycoprotein sialic acid in tricky matrices while optimizing your workflow.</p>
<h5><strong>9:00 AM CET: Opening Plenary: Untapped Potential in Ion Chromatography for Resolving Analytical Challenges</strong></h5>
<ul>
<li>Detlef Jensen (Thermo Fisher Scientific, IC Expert)</li>
</ul>
<h5><strong>11:00 AM CET: Determination of Chlorate in Agar Samples Using Ion Chromatography</strong></h5>
<ul>
<li>Flavio Zanetti (Biopolife Srl, Trieste, Italy)</li>
</ul>
<h5><strong>12:00 PM CET: Determination of Sulphur Dioxide in Foods by Ion Chromatography</strong></h5>
<ul>
<li>Noel Finn (Public Analyst Laboratory, St. Finbarr’s Hospital, Douglas Road, Cork)</li>
</ul>
<h5><strong>1:00 PM CET: 30 min Lunch</strong></h5>
<h5><strong>1:30 PM CET: Analysis of Polar Cationic Pesticides by IC-MS</strong></h5>
<ul>
<li>Anatte Margalit (DAFM)</li>
</ul>
<h5><strong>2:30 PM CET: Determination of Glycoprotein Sialic Acid Composition Using HPAE-PAD in Dual Eluent Generation Cartridge Mode</strong></h5>
<ul>
<li>Jingli Hu (Thermo Fisher Scientific, IC/SP Applications, Sunnyvale)</li>
</ul>
<h5><strong>3:30 PM CET: Optimizing Your IC Workflow Using Chromeleon CDS</strong></h5>
<ul>
<li>Dan Talbot (Thermo Fisher Scientific, IC/SP Senior, Application Sales Support, UK &amp; Ireland)</li>
</ul>


IC Symposium 2023 - Day 2

Join us for the 2023 Global IC Symposium! Europe and Africa

<p><strong>This year we invite you to ignite your mind to Be Curious about what Ion chromatography (IC) can do with its versatility in many detections across the IC application spectrum to improve your workflows and solve challenges.</strong></p>
<p>During this three day virtual event, learn from a panel of experts how the latest IC applications are helping colleagues achieve breakthroughs, and walk away with valuable information on how advancements in IC can increase laboratory analytical capabilities. </p>
<p>Although each day is convenient for a specific region, you can attend any session on any day. All sessions and workshops will be available for on-demand viewing after the event concludes.</p>
<h3><strong>Day 3 Agenda | April 20 | Asia Pacific</strong></h3>
<p>Expand the world of electrochemistry applications and IC hyphenation with high-resolution mass spectrometry and understand how Inductively Coupled Plasma Spectrometry can speciate isotopes while simplifying Pharmacopeia QC analysis and keeping your IC in top-notch condition.</p>
<h5><strong>11:00 AM SGT: Opening Plenary: Electrochemical Detection and Its Application in Ion Chromatography</strong></h5>
<ul>
<li>Yan Liu (Thermo Fisher Scientific, IC/SP R&amp;D, Sunnyvale)</li>
</ul>
<h5><strong>12:00 PM SGT: Application of Anion Chromatography Coupled High-Resolution Mass Spectrometry to Increase Cellular Metabolite Coverage</strong></h5>
<ul>
<li>Vinzenz Hofferek (McConville Lab, Department of Biochemistry and Pharmacology)</li>
</ul>
<h5><strong>1:00 PM SGT: Analysis of Anhydrosugars and Xylooligosaccharides by Ion Chromatography with Electrospray Lithium Cationisation-Triple Quadrupole Tandem Mass Spectrometry</strong></h5>
<ul>
<li>Dr. Estrella Sanz Rodriguez (Natural Products Analysis, Australian Centre for Research on Separation Science (ACROSS), University of Tasmania)</li>
</ul>
<h5><strong>2:00 PM SGT: You Gotta Keep ‘Em Separated: Novel IC Applications for Subsequent Isotopic Analyses by Rapid and/or Hyphenated Techniques</strong></h5>
<ul>
<li>Dr. Brandon Mahan (College of Science and Engineering, James Cook University)</li>
<li>Grace Manestar (IsoTropics PhD Candidate)</li>
</ul>
<h5><strong>3:00 PM SGT: Use of Ion Chromatography in Pharmaceutical Quality Control</strong></h5>
<ul>
<li>Catherine Frankis and Stella Planou (Reading Scientific Services Ltd., Reading Science Centre, Whiteknights Campus)</li>
</ul>
<h5><strong>4:00 PM SGT: Tips on Keeping Your Ion Chromatography in Top-Notch Condition</strong></h5>
<ul>
<li>Ryan Ong (Thermo Fisher Scientific, IC/SP Application Sales Support, Singapore)</li>
</ul>


IC Symposium 2023 - Day 3

Join us for the 2023 Global IC Symposium! Day 3 | Asia Pacific.

<p><strong>This meeting covers the latest trends and techniques to meet current water and environmental testing demands for emerging contaminants and evolving regulations. Our guest speakers from the water industry and environmental research and testing laboratories will share their expertise on their latest work, analytical workflows, and automation.</strong></p>
<p>Join us and discover valuable insights to maximise testing productivity and instrument uptime, simplify workflows and standardise data workup.</p>
<h3><strong>June 27 (Day 1) Emerging contaminants</strong></h3>
<p>This session will explore PFAS, microplastics, untargeted screening, automation and sample preparation.</p>
<p>Attend the whole day or selected presentations only.</p>
<h5><strong>10:00</strong> Online access opens (Streaming begins 09:30 BST/10:30 CEST)</h5>
<h5><strong>10:30 Opening of the event, welcome and introduction</strong></h5>
<ul>
<li>Presenter: Jeff Stubbs (Thermo Fisher Scientific)</li>
</ul>
<h5><strong>10:50 Screening of unknowns using Thermo Scientific Orbitrap MS and Thermo Scientific Compound Discoverer software</strong></h5>
<ul>
<li>Presenter: Janine Elliot (Scottish Environment Protection Agency)</li>
</ul>
<h5><strong>11:20 Biotoxins, seafood safety and mass spectrometry</strong></h5>
<ul>
<li>Presenter: Ben Maskrey (Centre of Environment, Fisheries and Aquaculture Science)</li>
</ul>
<h5><strong>11:50 Determination of microplastics with pyrolysis and GC-MS</strong></h5>
<ul>
<li>Presenter: Dr. Michael Soll (Frontier Lab Europe)</li>
</ul>
<h5><strong>12:20</strong> Break</h5>
<h5><strong>12:40 What combustion ion chromatography sum parameter analysis can do for the analysis of PFAS</strong></h5>
<ul>
<li>Presenter: Dr. Lewis Mark (A1-envirosciences Limited)</li>
</ul>
<h5><strong>13:10 Use of Thermo Scientific Exploris 120 Orbitrap HRAM-LC-MS for the analysis of 47 PFAS compounds in UK waters</strong></h5>
<ul>
<li>Presenter: John Quick (ALS Environmental Ltd.)</li>
</ul>
<h5><strong>13:40 Workshop - new untargeted PFAS analysis workflow capabilities within Compound Discoverer software</strong></h5>
<ul>
<li>Presenter: Frans Schoutsen (Thermo Fisher Scientific)</li>
</ul>
<h5><strong>14:10</strong> Break</h5>
<h5><strong>15:10 Starting your voyage into PFAS? Tools and protocols to succeed</strong></h5>
<ul>
<li>Presenter: Ed George (Thermo Fisher Scientific)</li>
</ul>
<h5><strong>16:10 Workshop - new approaches in performing automation by GC (and LC)</strong></h5>
<ul>
<li>Presenter: Dan Carrier (Thermo Fisher Scientific)</li>
</ul>
<h5><strong>16:30</strong> Closing of the live streaming event</h5>


Thermo Scientific: Water & Environmental Analysis User Meeting - Day 1

Water & Environmental Analysis User Meeting - Day 1

Streaming live from our Demo Centre in Hemel Hempstead | Join the complete event or the presentations of your choice only.

<p><strong>This meeting covers the latest trends and techniques to meet current water and environmental testing demands for emerging contaminants and evolving regulations. Our guest speakers from the water industry and environmental research and testing laboratories will share their expertise on their latest work, analytical workflows, and automation.</strong></p>
<p>Join us and discover valuable insights to maximise testing productivity and instrument uptime, simplify workflows and standardise data workup.</p>
<h3><strong>June 28 (Day 2) – Evolving regulations</strong></h3>
<p>This session will explore disinfection by-products, haloacetic acids, heavy metals, volatiles/semi-volatiles analysis.</p>
<p>Attend the whole day or selected presentations only.</p>
<h5><strong>10:00</strong> Online access opens (Streaming begins 09:30 BST/10:30 CEST)</h5>
<h5><strong>10:30 Opening of the event, welcome and introduction</strong></h5>
<ul>
<li>Presenter: Ed George (Thermo Fisher Scientific)</li>
</ul>
<h5><strong>10:50 Beyond compliance - use of the Orbitrap Exploris 120 HRAM-LC-MS for the monitoring of catchments</strong></h5>
<ul>
<li>Presenter: Mark Collins (Northern Ireland Water)</li>
</ul>
<h5><strong>11:20 Utilizing IC-MS/MS for the analysis of halo acetic acids and disinfection by products in drinking water</strong></h5>
<ul>
<li>Presenter: Chris Pegg (Anglian Water)</li>
</ul>
<h5><strong>11:50 GC-MS analysis of environmental samples using automated sample preparation</strong></h5>
<ul>
<li>Presenter: John Quick (ALS Environmental Ltd.)</li>
</ul>
<h5><strong>12:20</strong> Break</h5>
<h5><strong>12:40 Development of an ion chromatography method with EC and MS detection for the analysis of bromide and the disinfection by products bromate, chlorate and chlorite</strong></h5>
<ul>
<li>Presenter: Joseph Scott (Affinity Water)</li>
</ul>
<h5><strong>13:10 High throughput nutrients analysis - developing methodologies for environmental and wastewater matrices</strong></h5>
<ul>
<li>Presenter: Red Godwin (Thermo Fisher Scientific)</li>
</ul>
<h5><strong>13:40 Performing the analysis of trace metals in environmental samples via ICP and ICP-MS</strong></h5>
<ul>
<li>Presenter: Trudy McMurray (Northern Ireland Environment Agency)</li>
</ul>
<h5><strong>14:15</strong> Closing of the live streaming event</h5>


LCMS webinars focusing on IC - page 7

Water & Environmental Analysis User Meeting - Day 2