Meeting EU Drinking Water Standards: The Critical Role of Sample Prep in Trace Contaminant Analysis

<h3>Charged for Action: The Hidden Secrets of AEX Oligonucleotide Analysis</h3>Oligonucleotides—short, well-defined fragments of DNA or RNA—have become indispensable tools in modern life sciences. They underpin cutting-edge applications in molecular diagnostics, gene therapy, and pharmaceutical development. Before oligonucleotides can be confidently used in research or clinical settings, however, their quality must be rigorously assessed. Purity, sequence integrity, length distribution, and chemical modifications all play a decisive role in their performance.<img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/KNAUER_Oligos_Made_Easy_Part_2_AEX_2_45eee54fa0.jpg" alt="KNAUER: Oligos Made Easy - Part 2: AEX" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_KNAUER_Oligos_Made_Easy_Part_2_AEX_2_45eee54fa0.jpg 245w," sizes="100vw" width="1534" height="711">Among the analytical techniques available today, anion exchange chromatography (AEX) stands out as a robust and highly informative method for characterizing oligonucleotides. By exploiting their intrinsic negative charge, AEX provides detailed insight into product composition and impurity profiles that are difficult to obtain by other approaches.<blockquote><a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/article/6808">Part 1 of the Oligos series: A Practical Guide to Oligonucleotide Production – From Concept to Final Product</a></blockquote><h4>How Scientists Read Oligonucleotides Using Charge</h4>At the core of AEX lies a simple but powerful principle: oligonucleotides carry a negatively charged phosphate backbone. This charge increases in a predictable way with strand length and, to a lesser extent, sequence composition. Anion exchange chromatography uses this property to separate oligonucleotides based on differences in charge strength and interaction with a positively charged stationary phase.As a result, AEX has become one of the most widely used analytical tools for evaluating oligonucleotide quality. It allows scientists to distinguish full-length products from truncated species, degradation products, or closely related variants—often with single-nucleotide resolution.<h4>Why Ion Exchange Works: Binding, Retention, and Release</h4>Ion exchange chromatography (IEX) relies on electrostatic interactions between analytes and a charged stationary phase. In the case of oligonucleotides, anion exchange is used, where negatively charged molecules interact with positively charged functional groups on the resin—most commonly quaternary amines.The system consists of two main components:<ul><li>Stationary phase: A positively charged resin that binds anionic analytes.</li><li>Mobile phase: An aqueous buffer system, typically combined with a salt gradient, that controls both pH and elution strength.</li></ul>Retention in AEX is governed primarily by charge. Molecules with weaker overall charge interact less strongly with the stationary phase and elute earlier, while more highly charged oligonucleotides bind more tightly and require stronger elution conditions.<img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/KNAUER_Oligos_Made_Easy_Part_2_AEX_Figure_1_IEX_overview_including_elution_order_dependency_on_analyte_charge_3419ad3075.jpg" alt="KNAUER: Oligos Made Easy - Part 2 AEX: Figure 1 IEX overview including elution order dependency on analyte charge." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_KNAUER_Oligos_Made_Easy_Part_2_AEX_Figure_1_IEX_overview_including_elution_order_dependency_on_analyte_charge_3419ad3075.jpg 245w," sizes="100vw" width="1583" height="654"><h4>The Five-Step Workflow of Ion Exchange Chromatography</h4>AEX separations generally follow a well-established sequence of steps:<ol><li>Column equilibration The column is conditioned with the starting buffer until the baseline and pH are stable. This ensures that all charged sites on the resin are ready for interaction.</li><li>Sample loading Samples, prepared in the starting buffer, are introduced under conditions that promote binding of the target oligonucleotides while minimizing retention of unwanted impurities.</li><li>Washing The column is washed with the starting buffer to remove uncharged or weakly interacting components. The baseline stabilizes as these species are flushed away.</li><li>Elution Bound oligonucleotides are released using a salt or pH gradient. Increasing ionic strength introduces competing ions that displace the analytes from the resin. Weakly bound species elute first; strongly charged oligonucleotides elute later.</li><li>Regeneration and re-equilibration A high-salt buffer removes any remaining bound material. The column is then returned to starting conditions in preparation for the next analysis.</li></ol><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/KNAUER_Oligos_Made_Easy_Part_2_AEX_Figure_2_Five_steps_of_IEX_procedure_in_blue_outlined_using_an_example_chromatogram_in_red_38dd70b1b1.jpg" alt="KNAUER: Oligos Made Easy - Part 2 AEX: Figure 2 Five steps of IEX procedure (in blue), outlined using an example chromatogram (in red)." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_KNAUER_Oligos_Made_Easy_Part_2_AEX_Figure_2_Five_steps_of_IEX_procedure_in_blue_outlined_using_an_example_chromatogram_in_red_38dd70b1b1.jpg 245w," sizes="100vw" width="1583" height="674"><h4>Why AEX Is Especially Effective for Oligonucleotides</h4>The repeating phosphate backbone of oligonucleotides gives rise to a strong and uniform anionic character across a wide pH range (typically pH 7–9). This makes oligonucleotides exceptionally well suited for anion exchange separations.Key factors influencing AEX behavior include:<ul><li>Charge density: Longer oligonucleotides carry more negative charges and therefore exhibit stronger retention.</li><li>Resin chemistry: Strong and weak anion exchangers differ in functional groups and pH stability, influencing selectivity and robustness.</li><li>Buffer composition: The choice of buffering ions and salt gradients directly affects elution strength and resolution.</li></ul>Salt gradients—commonly based on chloride ions—compete with the oligonucleotide backbone for binding sites on the resin. By carefully tuning gradient slope and composition, analysts can achieve highly reproducible and finely resolved separations.<img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/KNAUER_Oligos_Made_Easy_Part_2_AEX_Figure_3_Ionic_interactions_and_p_H_influence_on_Anion_Exchange_Chromatography_AEX_612e231ba4.jpg" alt="KNAUER: Oligos Made Easy - Part 2 AEX: Figure 3 Ionic interactions and pH influence on Anion Exchange Chromatography (AEX)." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_KNAUER_Oligos_Made_Easy_Part_2_AEX_Figure_3_Ionic_interactions_and_p_H_influence_on_Anion_Exchange_Chromatography_AEX_612e231ba4.jpg 245w," sizes="100vw" width="1547" height="593"><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/KNAUER_Oligos_Made_Easy_Part_2_AEX_Figure_4_AEX_buffer_selection_and_gradient_strategy_chromatogram_with_red_buffer_gradient_curve_53fd1bb11b.jpg" alt="KNAUER: Oligos Made Easy - Part 2 AEX: Figure 4 AEX buffer selection and gradient strategy (chromatogram with red buffer gradient curve)." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_KNAUER_Oligos_Made_Easy_Part_2_AEX_Figure_4_AEX_buffer_selection_and_gradient_strategy_chromatogram_with_red_buffer_gradient_curve_53fd1bb11b.jpg 245w," sizes="100vw" width="1600" height="327"><h4>AEX as the Gold Standard for Oligonucleotide Separation</h4>Anion exchange chromatography has become the method of choice for both analytical and preparative oligonucleotide workflows. Its advantages include:<ul><li>High resolving power: Closely related impurities, including n–1 or n–2 species, can often be separated and quantified.</li><li>Predictable retention behavior: In many cases, retention time correlates linearly with oligonucleotide length, simplifying identification.</li><li>Compatibility with modified oligonucleotides: Even chemically modified sequences retain their anionic nature, allowing AEX to resolve both products and related impurities.</li><li>Excellent control via gradient elution: Salt gradients provide precise tuning of selectivity and resolution.</li></ul>Typically, shorter or degraded oligonucleotides elute at lower ionic strength, while full-length products with higher charge density appear later in the chromatogram.<img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/KNAUER_Oligos_Made_Easy_Part_2_AEX_Figure_5_AEX_Advantages_and_Challenges_7835521e34.jpg" alt="KNAUER: Oligos Made Easy - Part 2 AEX: Figure 5 AEX Advantages and Challenges." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_KNAUER_Oligos_Made_Easy_Part_2_AEX_Figure_5_AEX_Advantages_and_Challenges_7835521e34.jpg 245w," sizes="100vw" width="1535" height="707"><h4>Final Perspective: Mastering AEX with Confidence</h4>Anion exchange chromatography may appear complex at first, but with thoughtful optimization of pH, buffer composition, and gradient conditions, it becomes a highly precise and reliable tool for oligonucleotide analysis. When properly applied, AEX delivers sharp peaks, excellent resolution, and robust performance—even for challenging sequences and modifications.For laboratories developing or refining oligonucleotide workflows, AEX offers both analytical depth and operational confidence.<blockquote>If you would like to discuss AEX method development or optimization for oligonucleotides, feel free to contact us at sales@knauer.net. More insights into practical oligonucleotide analysis will follow in our “Oligos Made Easy” series.For technical questions or in-depth discussion, the author can be reached at kindler@knauer.net</blockquote>

Oligos Made Easy - Part 2: AEX

Although modern agriculture needs to be based on accurate real-time data on soil, plant, and environmental conditions, affordable, sufficiently sensitive, and robust monitoring devices for this purpose are currently missing. According to scientists, low-cost sensors based on new smart materials and printing technologies offer a suitable solution. The results of multidisciplinary research thus far and proposals for the development of these devices have been presented by researchers from Czechia, Great Britain, Spain, and the USA in a review article in the prestigious journal <a target="_blank" rel="noopener noreferrer" href="https://www.nature.com/articles/s41467-026-68778-6#Abs1">Nature Communications</a>.“Sensor technologies for monitoring plant health are still in their infancy. Only simple sensors capable of monitoring basic parameters such as pH, soil moisture, or temperature are commonly available on the market. However, if farmers are to face current challenges, including climate change, and ensure sufficient food for humanity, they need much more effective tools that will enable them to monitor, for example, various pathogens, including bacteria and fungi. In our opinion, a suitable solution is a combination of new smart materials and printing for the production of inexpensive, durable, and scalable materials,” said the article’s first author, David Panáček from CATRIN, who worked on the study during his internship at Imperial College London.<img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/CATRIN_Soucasne_senzory_nestaci_Vedci_navrhuji_novou_cestu_ke_sledovani_zdravi_rostlin_b4014952a3.jpg" alt="CATRIN: David Panáček" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_CATRIN_Soucasne_senzory_nestaci_Vedci_navrhuji_novou_cestu_ke_sledovani_zdravi_rostlin_b4014952a3.jpg 208w," sizes="100vw" width="1536" height="1152">Sensors for monitoring plant health, for example, lag behind similar devices used in human medicine. According to the authors, advances in materials science, which will enable the development of scalable sensor technologies, play a key role in this potential shift. “Printing techniques, including inkjet printing, screen printing, aerosol printing, 3D printing, and direct laser writing, offer flexible ways to produce flexible sensors, large-area sensors, and sensors integrated directly into plants. The review article summarizes recent advances in printable low-dimensional materials for agricultural sensors, analyzes their physico-chemical properties in relation to sensor performance, and discusses key challenges and future opportunities requiring interdisciplinary integration,” said one of the corresponding authors, Michal Otyepka.The research brought together materials scientists, chemists, biologists, sensing experts, and printing technology specialists. Materials research and the use of graphene-based smart materials were led by scientists from CATRIN at Palacký University and VŠB-Technical University of Ostrava. Plant biologists from CATRIN contributed their knowledge of plant development, their responses to stress conditions, and the monitoring of their properties. Researchers from Imperial College London took care of the electronics, while representatives from<a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/companies/36"> CEITEC – VUT</a> focused on printing technologies. The team also included leading experts in sensing from the Catalan Institute of Nanoscience and Nanotechnology in Barcelona, and Joseph Wang of the University of California San Diego, the “father of sensing and electrochemistry,” also contributed his extensive knowledge.“This is a groundbreaking study on this topic, which has managed to attract some truly big names in their respective fields. Last but not least, the study demonstrates that interdisciplinarity at CATRIN gives rise to completely new and promising research topics, such as plant sensing and its use for studying plant-environment interactions and smart agriculture,” added another corresponding author, Nuria De Diego.

Current sensors are not good enough. Scientists propose a new way to monitor plant health

Article

Environment

Scientific article

Science and research

Video

The Czech Advanced Technology and Research Institute (CATRIN) at Palacký University is a cutting-edge scientific hub dedicated to advancing research in the fields of nanotechnology, biotechnology, and biomedicine. At its core, CATRIN boasts outstanding scientific teams, featuring international researchers. We emphasize interdisciplinarity, foster global collaborations, and work to translate our research findings into practical applications.

CATRIN

Organomation, founded in 1959, designs and manufactures high-quality nitrogen evaporators and extraction systems. Known for innovation and durability, their lab instruments are used globally for efficient sample preparation with strong customer support.

Organomation

Based in Berlin, KNAUER is a medium-sized, owner-managed company that has been serving the sciences since 1962. We develop and manufacture scientific instruments of superior quality for liquid chromatography, including: Analytical HPLC/UHPLC, Preparative HPLC, Fast protein liquid chromatography (FPLC), Multi-column chromatography/Simulated moving bed (SMB) chromatography. High pressure dosing, in-line detection systems, and osmometry are additional business areas.

KNAUER

Phenomenex

Thermo Fisher Scientific

Restek Corporation

Waters Corporation

PhenoAcademy Webinars
 Liquid Chromatography Series
<h4>HPLC Method Development: Practical Approaches for Identifying Equivalent and Orthogonal Columns</h4>
<ul>
<li>Recognize columns exhibiting comparable selectivity to your current column selection</li>
<li>Identify columns with different or orthogonal selectivity to your current column of choice</li>
<li>Understand the resources that are available for selectivity data for HPLC columns</li>
</ul>
<blockquote>
Presenter: Kevin Zhao (Senior Technical Specialist)
</blockquote>
<blockquote>
Kevin Zhao is the Senior Technical Specialist with Phenomenex and has 5 years of experience in biochemistry research; 4 years of Protein/Cancer research with CSULA-City of Hope Cancer Collaborative and over 9 years in Phenomenex. Mr. Zhao is a member of a global Technical Support Team which includes providing problem-solving, troubleshooting, and applications assistance for customers worldwide in the fields of chromatography (liquid and gas) and sample preparation, and also handling Industries supported include – pharmaceutical and biopharmaceutical, clinical research and clinical toxicology, food safety, environmental, fuels and specialty chemicals. Mr. Zhao received his B.S. in Biology at the California State Polytechnic University, Pomona. earned his M.S. in Biochemistry at California State University, Los Angeles, and also earned a MBA at the University of the Incarnate Word.
</blockquote>
<blockquote>
Presenter: Koen Askamp (Technical Specialist)
</blockquote>
<blockquote>
Koen is from the Netherlands, studied Molecular Life Sciences at Wageningen University (Netherlands), majoring in immunology. Analytical Chemistry was one of my favorite courses – who doesn’t love solving puzzles?
</blockquote>
<blockquote>
I like drumming, scuba diving, motorcycle-riding, and this past winter I learned how to ski (a little)!
</blockquote>
<blockquote>
Presenter: Namrata Saxena (Technical Manager)
</blockquote>
<blockquote>
Namrata Saxena is a Technical Manager - Asia/Pacific” with Phenomenex and is based in Sydney, Australia. She has a post graduate degree with a specialization in Analytical Chemistry, with extensive experience in the Liquid chromatography domain, and her current focus is in Omics and Biologics field. 
</blockquote>
<blockquote>
Before joining Phenomenex she had worked as “Product Specialist” and spent several years in column chemistry, column manufacturing and method development in the application and manufacturing labs of Bischoff Chromatography, Germany. 
</blockquote>
<blockquote>
In her current role, she provides product selection, method development, training and troubleshooting support to customers as well as Phenomenex Sales Consultants globally. 
</blockquote>

Phenomenex: PhenoAcademy Webinars - Liquid Chromatography Series

HPLC Method Development: Practical Approaches for Identifying Equivalent and Orthogonal Columns

Recognize columns exhibiting comparable selectivity to your current column selection.

Identification of Equivalent and Orthogonal HPLC Columns

Since 2018 when the presence of Nitrosamines was first reported in several ARB (angiotensin II receptor blocker) drugs, there has been increasing regulatory focus to mitigate the presence of these potential human carcinogens in drug substances and drug products.
<h5>Who Should Attend:</h5>
<ul>
<li>Anyone with an interest in, or challenged by, the analysis N-Nitrosamines and Nitrosamine Drug Substance Related Impurities (NDSRI) in API and/or Drug Products
</li>
<li>Analysts and Scientists working in the Pharmaceutical and Generic Pharmaceutical industries.
</li>
</ul>
<h5>Key Learning Points:</h5>
<ul>
<li>Review the latest regulatory updates (FDA and EMA)
</li>
<li>Learn about published LC-MS/MS applications which address the N-Nitrosamines and NDSRI analysis challenges in drug products
</li>
<li>Find solutions to sample preparation challenges for N-Nitrosamines analysis in drug products
</li>
<li>Obtain examples of specific Phenomenex applications for nitrosamines (10 mutagenic nitrosamine impurities in Acyclovir) and NDSRIs (N-nitroso propranolol in a propranolol drug substance and product)
</li>
</ul>
<blockquote>
Presenter: Phil Koerner (Market Development Manager, Pharmaceuticals)
</blockquote>

Phenomenex: Nitrosamines in Drug Products: Where Are We Now?

Nitrosamines in Drug Products: Where Are We Now?

Learn about published LC-MS/MS applications which address the N-Nitrosamines and NDSRI analysis challenges in drug products

AAVs are attractive vehicles for targeted drug delivery. There are numerous Critical Quality Attributes (CQAs) evaluated to ensure safety and efficacy of AAVs. Aggregation is a CQA and should be controlled as AAVs are prone to aggregation from freeze thaw cycles during storage.
<ul>
<li>In this webinar, we will explore CQAs using a novel size exclusion column optimal for AAV analysis, which enables the separation of AAV monomer from aggregate species with excellent peak shape, resolution and peak profile to provide better data quality. Due to both the inert surface chemistry and flow-path in the BiozenTM dSEC-7 column, the analysis conditions were independent of the serotype showcasing the utilization of platform methodology.</li>
<li>In addition to calculating the aggregation level, aggregate molar mass, molar masses of protein and nucleic acid, and extinction coefficients of capsid and DNA were also determined. The novel particle chemistry of Biozen dSEC-7 provides instant stabilization of sensitive detectors like MALS to provide quality data.</li>
<li>Additionally, Full-to-Empty capsid ratio was determined to accurately predict capsid load demonstrating Biozen dSEC-7’s utility as a holistic solution for the determination of CQAs of AAVs.</li>
</ul>
Key Learning Points:
<ul>
<li>Learn how to optimize AAV aggregate analysis.</li>
<li>Understand how size exclusion can be effectively utilized for capsid load quantification.</li>
<li>Find out how optimized column specifications can improve data quality to determine critical quality attributes</li>
</ul>
Who should attend?
Any scientist, associate or manager that optimizes AAV aggregate analysis methods.
<blockquote>
Presenter: Claire Butré, PhD, (R&amp;D Technical Leader, Quality Assistance &amp; Ramkumar Dhandapani, PhD, Senior Product Management, Phenomenex)
</blockquote>

Phenomenex: Evaluation of AAV Aggregation & Capsid Load (Empty / Full) CQAs

Evaluation of AAV Aggregation & Capsid Load (Empty / Full) CQAs

Explore CQAs using a novel SEC column for AAV analysis, which enables the separation of AAV monomer from aggregate species.

Evaluation of AAV Aggregation & Capsid Load CQAs

<h4>Event Overview:</h4>
In this webinar, we present an mRNA characterization workflow that includes sample preparation, LC-MS methodology and bioinformatics processing for the evaluation of 5&#39; Cap efficiency, mapping of oligonucleotide sequence fragments, isomer resolution and Poly (A) tail intact mass analysis.
Who Should Attend:
<ul>
<li>Researchers and Scientists: Ideal for those seeking to expand their insights into LC-MS characterization techniques and applications of mRNA modifications.</li>
<li>Quality Control and In-Process Testing Professionals: Recommended for professionals involved in quality control, in-process testing, or analytical development.</li>
<li>Biopharmaceutical Industry Professionals: Valuable for those evaluating and optimizing their current mRNA-based therapeutics development and characterization solutions.</li>
</ul>
Key Learning Points:
<ul>
<li>Explore the LC-MS characterization method for 5&#39; Cap, coding sequence mapping, and Poly(A) tail analysis.</li>
<li>Gain insights into utilizing ion-pairing reversed-phase LC-MS to characterize mRNA drug substances.</li>
<li>Discover the benefits of utilizing BioTi core-shell column technology to improve sensitivity and resolution for oligonucleotide identification.</li>
<li>Review of bioinformatics processing workflow</li>
</ul>
<blockquote>
Presenter: Roxana Eggleston-Rangel (Senior Applications Scientist, Phenomenex)
</blockquote>

Phenomenex: mRNA Characterization - From 5’ Cap to Poly (A): What IPRP–MS Can Tell You

mRNA Characterization - From 5’ Cap to Poly (A): What IPRP–MS Can Tell You

Presentation of mRNA characterization workflow including sample preparation, LC-MS methodology and bioinformatics processing.

mRNA Characterization: What IPRP–MS Can Tell You

<ul>
<li>Learn about physical and chemical properties of common reverse phase stationary phases</li>
<li>Find an understanding and recieve steps for stationary phase selection</li>
<li>Take a deep dive into mobile phase selectivity</li>
</ul>
<blockquote>
Presenter: Kevin Zhao (Senior Technical Specialist)
</blockquote>
<blockquote>
Kevin Zhao is the Senior Technical Specialist with Phenomenex and has 5 years of experience in biochemistry research; 4 years of Protein/Cancer research with CSULA-City of Hope Cancer Collaborative and over 9 years in Phenomenex. Mr. Zhao is a member of a global Technical Support Team which includes providing problem-solving, troubleshooting, and applications assistance for customers worldwide in the fields of chromatography (liquid and gas) and sample preparation, and also handling Industries supported include – pharmaceutical and biopharmaceutical, clinical research and clinical toxicology, food safety, environmental, fuels and specialty chemicals. Mr. Zhao received his B.S. in Biology at the California State Polytechnic University, Pomona. earned his M.S. in Biochemistry at California State University, Los Angeles, and also earned a MBA at the University of the Incarnate Word.
</blockquote>
<blockquote>
Presenter: Koen Askamp (Technical Specialist)
</blockquote>
<blockquote>
Koen Askamp is a Technical Specialist in Phenomenex. He was awarded his BSc and MSc degrees in Molecular Life Sciences at Wageningen University in the Netherlands and joined Phenomenex in 2020 as Technical Sales Consultant. Koen became part of Phenomenex’s global technical support team in 2022 and assists laboratories in training chromatographers and developing analytical methods.
</blockquote>
<blockquote>
Presenter: Namrata Saxena (Technical Manager)
</blockquote>
<blockquote>
Namrata Saxena is a Technical Manager - Asia/Pacific with Phenomenex and is based in Sydney, Australia. She has a post graduate degree with a specialization in Analytical Chemistry, with extensive experience in the Liquid chromatography domain, and her current focus is in Omics and Biologics field. 
</blockquote>
<blockquote>
Before joining Phenomenex she had worked as “Product Specialist” and spent several years in column chemistry, column manufacturing and method development in the application and manufacturing labs of Bischoff Chromatography, Germany. 
</blockquote>
<blockquote>
In her current role, she provides product selection, method development, training and troubleshooting support to customers as well as Phenomenex Sales Consultants globally. 
</blockquote>

Phenomenex: HPLC Method Development: Practical Approaches for Identifying Equivalent and Orthogonal Columns

Learn about physical and chemical properties of common reverse phase stationary phases

HPLC Method Development: Approaches for Identifying Columns

Many chromatographers experience difficulty retaining and separating polar analytes. This will often require reanalysis using alternative methods or changing column chemistries. The pain can be minimized by understanding the mechanisms for polar analyte retention and using the proper column designed for these difficult separations.
<blockquote>
Presenter: Chris Collins, Ph.D. (Principal Product Marketing Manager, Waters Corporation)
</blockquote>
<blockquote>
Chris Collins, Ph.D. earned his Ph.D. in Analytical Chemistry from North Carolina State University in 2014. He has over 20 years experience in the pharmaceutical industry with a focus on LC/MS Method Development. Chris started working at Waters Corporation in 2018 as a Chemistry Account Manager for North Carolina/South Carolina. In August, he transitioned to the role of Principal Product Marketing Manager for ACQUITY UPLC columns, UHPLC columns, Guards, and MaxPeak Premier Column Technology.
</blockquote>

Waters Corporation: Going Beyond the Boundaries of Retaining and Separating Polar Compounds

Going Beyond the Boundaries of Retaining and Separating Polar Compounds

The pain can be minimized by understanding the mechanisms for polar analyte retention and using the proper column designed for these difficult separations.

Retaining and Separating Polar Compounds

Chlorhexidine is a disinfectant and antiseptic widely used for skin disinfection prior to surgery, and for cleaning wounds or treating oral infections. The current European Pharmacopeia chlorhexidine digluconate monograph has proved problematic. In this webinar the proposed draft monograph is evaluated, considering multiple batches of HPLC columns, and also the impact of system dwell volume on the overall performance and robustness of the monograph method.
Who Should Attend:
Any LC laboratory managers, analysts looking to learn more about Chlorhexidine
Key Learning Points:
<ul>
<li>Find what is it required to achieve system suitability criteria in LC analysis</li>
<li>Learn why batch to batch reproducibility test important in method validation </li>
<li>Understand why and when must gradient delay volume be considered </li>
<li>Catch the importance of choosing the “right” column and “correct” gradient elution for separating a great number of impurities</li>
</ul>
<blockquote>
Presenter: Dr. Amra Perva (University of Maribor, Faculty of Chemistry and Chemical Engineering)
</blockquote>
<blockquote>
Amra is employed as a research fellow at the Faculty of Chemistry and Chemical Engineering at the University of Maribor (Slovenia). As the project lead in a GMP certified Contract Research Organization (Group for Separation Analysis) she has over a decade of experience working with pharmaceutical companies in the field of LC and GC method development, validation, stability and release testing for drug substance and drug product.
</blockquote>

Phenomenex: Batch Reproducibility and Effect of Gradient Delay Volume on Separation of Impurities of Chlorhexidine Digluconate

Batch Reproducibility and Effect of Gradient Delay Volume on Separation of Impurities of Chlorhexidine Digluconate

Separation of Impurities of Chlorhexidine Digluconate

Developing methods of analysis for drug compounds is not an easy task. Knowing how to develop a new method or how to incorporate new compounds into an existing method, as well as what is needed in terms of both materials and methods, can be challenging.
In this webinar, we will get you started on your analytical method with your column choice, including the particle, phase, and dimension. We’ll then discuss the mobile phase choice and method conditions that will meet your analytical goals. Throughout this training, we will use Restek’s Pro EZLC Chromatogram Modeler to exemplify the practices discussed.
<ul>
<li>Learn key information on how to effectively analyze drug compounds.</li>
<li>See how the Pro EZLC Chromatogram Modeler can help with adding new compounds to your analysis.</li>
<li>Learn how the Raptor Biphenyl allows for easy incorporation of these compounds into existing methods.</li>
</ul>

Restek Corporation: Method Development Considerations for the LC-MS/MS Analysis of Drugs

Method Development Considerations for the LC-MS/MS Analysis of Drugs

In this webinar, we will get you started on your analytical method with your column choice, including the particle, phase, and dimension.

Method Development for the LC-MS/MS Analysis of Drugs

Event Overview:
<ul>
<li>Principle of Chirality
</li>
<li>Chiral Stationary Phases (CSP)
</li>
<li>Polysaccharide CSPs and their Chiral Retention Mechanisms
</li>
<li>Chiral Method Development: Separation Modes and Screening Protocol
</li>
<li>SFC Chiral Method Development
</li>
<li>Chiral Method Optimization: Case Study
</li>
</ul>
Who Should Attend:
<ul>
<li>Any LC users who would like to understand how chiral separations work and how to apply the principles of chiral theory in developing an analytical method for separation of enantiomers.
</li>
<li>Any analyst who wishes to learn the basics of chiral theory.
</li>
<li>Any academic or pharmaceutical scientists who need to develop a method for separation and characterization of chiral compounds.
</li>
</ul>
Key Learning Points:
<ul>
<li>Learn what chiral compounds are and how they are separated using LC and what a chiral stationary phase (CSP) is and the different types there are
</li>
<li>Understand the three critical components of Polysaccharide CSPs and review the difference between coated and immobilized CSPs and how they are applied in different analytical contexts
</li>
<li>Learn how to approach a chiral screen using different separation modes.
</li>
</ul>
<blockquote>
Presenter: Laura K Ward (Technical Specialist, Phenomenex)
</blockquote>
<blockquote>
Laura Ward is a Technical Specialist for the Asia-Pacific region with Phenomenex, located in New Zealand. She completed an Honours thesis in the field of Geochemistry at the University of Sydney, Australia where she discovered a love for Analytical Chemistry. Following her academic pursuits, Laura joined Phenomenex Australia as a Technical Sales Consultant where she supported various industries and laboratories. After 5 years and a move to New Zealand, she has joined the Phenomenex Technical Team where she supports scientists globally with their method development, troubleshooting and application support. In her spare time Laura enjoys being outdoors in the New Zealand countryside and cooking from scratch, most recently venturing into cheesemaking!
</blockquote>

Phenomenex: LC Chiral Theory and Method Development

LC Chiral Theory and Method Development

Learn what chiral compounds are and how they are separated using LC and what a chiral stationary phase (CSP) is and the different types there are.

Are you tired of dealing with inconsistent LC results? Are you looking to extend the life of your LC columns and improve lab efficiency?
Our upcoming webinar, LC Columns Tips &amp; Tricks, is designed to help you get the most out of your LC columns.
Our expert will share invaluable insights on:
<ul>
<li>Understanding LC column chemistry to optimize separations
</li>
<li>Proper handling of new LC columns for maximum lifespan
</li>
<li>Proven techniques to extend column life and reduce downtime
</li>
<li>Effective column maintenance and troubleshooting strategies
</li>
</ul>
This live webinar is an excellent opportunity to deepen your knowledge, improve your lab efficiency, and reduce operational costs. Whether you&#39;re a seasoned pro or just getting started, you&#39;ll walk away with practical knowledge to elevate your column performance and lifetime. Adham Ahmed will be presenting and answering your questions live.
Who Should Attend?
<ul>
<li>Laboratory Analysts and Scientists who are involved in small molecule pharmaceutical analysis
</li>
<li>Chromatographers seeking to optimize LC column performance
</li>
<li>Quality control professionals aiming to improve data reliability and reproducibility
</li>
<li>Research and development scientists working on method development and validation
</li>
<li>Lab Managers: Leaders looking to improve operational efficiency and extend the lifespan of lab equipment.
</li>
<li>Anyone involved in LC column selection, maintenance, and troubleshooting
</li>
</ul>
<blockquote>
Presenter: Adham Ahmed (Global Product Manager, Thermo Fisher Scientific)
</blockquote>
<blockquote>
Adham Ahmed has 15+ years of experience in particle development and functionality for chromatographic application. Presently, he is the Global Product Manager of the Hypersil BDS and ODS and other columns within the Thermo Fisher Scientific Portfolio. His experience includes working in R&amp;D, application specialist and Product management, with a focus on understanding customer’s challenges and laboratory needs.
</blockquote>

Thermo Scientific: LC Columns Tips & Tricks

LC Columns Tips & Tricks

Our upcoming webinar, LC Columns Tips & Tricks, is designed to help you get the most out of your LC columns.

LCMS webinars focusing on Columns - page 9

LC Chiral Theory and Method Development

LC Columns Tips & Tricks

Method Development Considerations for the LC-MS/MS Analysis of Drugs

HPLC Method Development: Practical Approaches for Identifying Equivalent and Orthogonal Columns

Batch Reproducibility and Effect of Gradient Delay Volume on Separation of Impurities of Chlorhexidine Digluconate

Going Beyond the Boundaries of Retaining and Separating Polar Compounds

mRNA Characterization - From 5’ Cap to Poly (A): What IPRP–MS Can Tell You

HPLC Method Development: Practical Approaches for Identifying Equivalent and Orthogonal Columns

Evaluation of AAV Aggregation & Capsid Load (Empty / Full) CQAs

Nitrosamines in Drug Products: Where Are We Now?

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