News from LabRulezLCMS Library - Week 49, 2024

Metabolomics and sensory evaluation of white asparagus ingredients in instant soups unveil important (off-)flavours

<p><strong>Our Library never stops expanding. What are the most recent contributions to LabRulezLCMS Library in the week of 9th December 2024? Check out new documents from the field of liquid phase, especially HPLC and LC/MS techniques!</strong></p><blockquote><p><span style="font-size:20px;">👉 </span><a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/library"><span style="font-size:20px;"><strong>SEARCH THE LARGEST REPOSITORY OF DOCUMENTS ABOUT LCMS AND RELATED TECHNIQUES</strong></span></a></p></blockquote><blockquote><p><strong>👉 Need info about different analytical techniques?</strong> Peek into <a target="_blank" rel="noopener noreferrer" href="https://gcms.labrulez.com/library"><strong>LabRulezGCMS</strong></a> or <a target="_blank" rel="noopener noreferrer" href="https://icpms.labrulez.com/library"><strong>LabRulezICPMS</strong></a> libraries.</p></blockquote><p>This week we bring you applications by <strong>Shimadzu</strong>,<strong> Metrohm</strong>, and<strong> &nbsp;Waters Corporation</strong>, a presentation by <strong>Agilent Technologies</strong>, and a poster from <strong>MSACL </strong>by <strong>Thermo Fisher Scientific</strong>!</p><h4><strong>1. Shimadzu: </strong><a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/labrulez-bucket-strapi-h3hsga3/an_01_00750_en_bee79113e7.pdf"><strong>Analysis of Per- and Polyfluoroalkyl Substances (PFAS) using Triple Quadrupole Mass Spectrometer Part 1 - Fish Fillet</strong></a></h4><ul><li>Application</li></ul><p><strong>User Benefits</strong></p><ul><li>The optimized procedure for pretreatment and LC-MS/MS analytical conditions enable accurate quantification of thirty major PFASs targeted by AOAC SMPR from 0.1 μg/kg.</li><li>The method allows the initiation of PFASs analysis in food.</li></ul><p><strong>Introduction</strong></p><p>Per- and Polyfluoroalkyl Substances (PFASs) are a collective name for more than four thousand organofluorine compounds. Perfluorooctane sulfonic acid (PFOS) and perfluorooctanoic acid (PFOA) are representative compounds of PFAS. They are used in a wide range of applications, such as fire retardants, food packaging materials, and non-stick coatings, due to their water-repellent, oil-repellent, heat-resistant, and chemical-resistant properties. Due to their structural stability, PFAS widely remains in the environment. There are concerns about health risks caused by human ingestion of fish that have ingested seawater, river water, or feed contaminated with PFAS. Therefore, quantitative assessment of PFAS levels in fish should be important.</p><p>This application news introduces a quantitative analysis of PFAS in tuna fillets with LC-MS/MS. Thirty principal PFASs targeted by AOAC INTERNATIONAL, a North American organization that standardizes food testing methods and verifies analytical methods, were analyzed using newly created method, which was investigated from the pretreatment procedure then validated by recovery test. By reducing losses during the pretreatment and optimizing the analytical conditions, good recovery rates were obtained for all compounds.</p><p><strong>Conclusion</strong></p><p>This application news described a quantitative analysis of thirty PFAS targeted by AOAC INTERNATIONAL in tuna fillet. The analysis was performed using a quadrupole mass spectrometer LCMS-8060NX equipped with ultra-high performance liquid chromatograph Nexera X3 UHPLC system. Shim-pack Scepter, which provides good separation and peak shape, was used as the column. A spiked recovery test were conducted, and all compounds showed recovery rate within 80-120% and repeatability below 20% at spiked concentrations of 0.1, 1, 5 μg/kg. In particular, recovery rates of PFOS, PFOA, PFNA, and PFHxS were within 95.5-108.4% at all spiked concentrations. Using optimized pretreatment and analytical methods, accurate quantitation is possible from 0.1 μg/kg.</p><h4><strong>2. Agilent Technologies: </strong><a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/labrulez-bucket-strapi-h3hsga3/getting_the_most_from_your_diode_array_detector_aug292024_a46bb2eeed.pdf"><strong>Getting the Most from Your Diode Array Detector</strong></a></h4><ul><li>Presentation</li></ul><h5><strong>Agenda</strong></h5><ul><li><strong>Introduction</strong></li><li><strong>Fundamentals</strong><ul><li>Beer-Lambert law</li><li>UV-vis detection</li><li>DAD and MWD</li><li>DAD spectra collection</li><li>UV spectrum confirmation</li><li>peak purity</li></ul></li><li><strong>Maximizing sensitivity</strong><ul><li>What is sensitivity?</li><li>Factors affecting sensitivity</li><li>Analyte</li><li>Factors affecting sensitivity</li><li>System</li><li>Detector</li></ul></li><li><strong>Spectral acquisition</strong><ul><li>Settings</li><li>Spectrum storage</li><li>Range, step, and threshold</li><li>OpenLab Resources</li></ul></li><li><strong>DAD maintenance</strong><ul><li>Maintenance schedule</li><li>InfinityLab LC performance standard</li><li>Lab advisor</li><li>Care of flow cells</li><li>Flow cells</li><li>Best practices</li><li>Maintenance resources</li></ul></li><li><strong>Troubleshooting</strong></li><li><strong>Additional Resources</strong></li></ul><h4><strong>3. Waters Corporation: </strong><a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/labrulez-bucket-strapi-h3hsga3/720008521_en_ff59413ad8.pdf"><strong>HPLC Autosampler Performance II: Improved Injection Precision of USP Methods With the Alliance™ iS HPLC System</strong></a></h4><ul><li>Application</li></ul><p><strong>Abstract</strong></p><p>Method conditions and instrument characteristics can affect the autosampler performance of High-Performance Liquid Chromatography (HPLC) systems. Challenging method conditions, such as low injection volume and highly organic sample diluents, require high precision to meet strict suitability requirements. Instrument characteristics, such as sample aspiration mechanism and injector draw rate, often vary between vendors and may impact the precision performance of the autosampler. This can pose a challenge for analytical laboratories that desire to run methods with strict precision suitability criteria across HPLC systems from different vendors. In this study, the injection precision performance of the Alliance iS HPLC System and similar HPLC systems were compared using four compendial HPLC methods from the United States Pharmacopeia (USP) with strict precision criteria and challenging method conditions. Peak area reproducibility was evaluated as a proxy for autosampler performance of each system.</p><p><strong>Benefits</strong></p><ul><li>High precision for a range of sample diluents and injection volumes on the Alliance iS HPLC System</li><li>Meets strict injection precision criteria for challenging USP methods on the Alliance iS HPLC System</li><li>Demonstrates improved precision on the Alliance iS HPLC System over similar HPLC systems for highly organic sample diluent</li></ul><p><strong>Introduction</strong></p><p>For many regulated methods in the United States Pharmacopeia (USP), injection precision is a common system suitability criterion. Injection precision is a measure of the autosampler performance in a high-performance liquid chromatography (HPLC) system. The autosampler is responsible for delivering precise aliquots of sample to the HPLC system flow path.<sup>1</sup> This performance can be impacted by method conditions and instrument characteristics.<sup>2,3,4</sup> To ensure that acquired data is accurate and meets suitability requirements of the method, high performance is required from an autosampler.</p><p>Instrument characteristics, such as sample aspiration mechanism and injector draw rate, vary among vendors due to differences in system design. Sample aspiration is typically controlled by either a metering device or a sampling syringe.<sup>1</sup> Injector draw rate is a setting typically set to a default speed that often varies with instrument vendor.<sup>4</sup> These different characteristics can complicate running methods across systems from different vendors. A routine method that performs well on one HPLC system may not meet system suitability criteria on a different HPLC system. Therefore, it is important to consider how autosampler performance is affected by instrument characteristics.</p><p>In this study, four USP Assay monographs were used to examine the impact of instrument characteristics on injection precision of an Alliance iS HPLC System and comparable HPLC systems. The HPLC systems include sampling syringe and metering device aspiration mechanisms and utilize different injector draw speeds. The USP Assay methods have challenging method conditions: varying organic content in the sample diluent (20%–100% organic), low injection volumes (6.6 μL–20 μL), and narrow injection precision criteria (0.73% to 2.0%).</p><p><strong>Conclusion</strong></p><p>The ability of an HPLC system to acquire consistent, repeatable data is critical and requires high performance from an autosampler. This study examined the impact of instrument characteristics, such as sample aspiration mechanisms and injector draw rates, on HPLC performance in meeting precision requirements. Autosampler performance is complex and was found to vary with the method and the system. The metering device has benefits for precision performance over the sampling syringe, using a more finely tuned syringe draw motor and being located in-line with the high-pressure flow path.</p><p>For fluconazole and ketoconazole USP assays, the Alliance iS HPLC demonstrated the best performance overall, with the lowest peak area %RSDs. The ketoconazole assay had particularly challenging method conditions and produced widely varying results among the HPLC systems. Alliance iS HPLC System demonstrated high precision and produced consistent and reproducible results across the three-day period, meeting the stringent 0.73% peak area RSD requirement.</p><p>The ketoconazole assay produced disparate results among the HPLC systems using metering devices, which was unexpected. Upon tracking the absolute peak area of ketoconazole for each system, evaporation and carryover were ruled out as causes of the peak variability. Additionally, despite identical injector draw rates on two of the HPLC systems, their performance varied significantly. While aspiration mechanism and injector draw rate are important factors, they do not fully explain the observed variations in autosampler performance.</p><h4><strong>4. Metrohm: </strong><a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/labrulez-bucket-strapi-h3hsga3/an_c_198_download_6dcbc46fdf.pdf"><strong>Calcium acetate assay in calcium acetate capsules</strong></a></h4><ul><li>Application</li></ul><p><strong>Method validation according to the U.S. Pharmacopeia (USP)</strong></p><p>Calcium acetate functions as a phosphate binder in the gastrointestinal tract, helping to lower high phosphate levels in individuals with kidney disease who are receiving dialysis treatment [1,2]. To meet the stringent quality standards for pharmaceutical products, manufacturers and laboratories must use validated methods from the United States Pharmacopeia – National Formulary (USP-NF). Previously, such methods included titration or liquid chromatography (LC) with UV detection. As part of their modernization efforts, the USP has updated the calcium monograph to include ion chromatographic (IC) analysis, which is more straightforward and sensitive than previous methods. For the calcium acetate assay, the USP specifies ion chromatography using a cation-exchange column with L76 column material and non-suppressed conductivity detection to quantify the amount of calcium ions in calcium acetate capsules [3].</p><p>The current IC method employs a Metrosep C 6 -150/4.0 column (L76) to separate calcium from other ions in calcium acetate capsules. This method has been validated according to USP General Chapters &lt;621&gt; Chromatography [4] and &lt;1225&gt; Validation of Compendial Procedures [5]. All acceptance criteria for the calcium acetate assay in the USP monograph «Calcium Acetate Capsules» are met.</p><p><strong>SAMPLE AND SAMPLE PREPARATION</strong></p><p>The standard solution nominally contains 0.08 mg/mL of USP Calcium Acetate Reference Standard (Cat#1086334) in water. It is prepared by accurately weighing 80.0 mg of USP Calcium Acetate Reference Standard and transferring it into a clean 1000 mL volumetric flask. It is dissolved and made up to the mark with ultrapure water (UPW).</p><p>For sample stock solutions nominally containing 6.7 mg/mL of calcium acetate, an appropriate portion of the contents of at least 20 capsules are transferred<br>into a 2000 mL volumetric flask. UPW is added to about 40% of the final volume of the volumetric flask, and the solution is then sonicated for 30 minutes with intermittent shaking. Afterwards, the solution is made up to the mark with UPW and filtered through 0.2 μm filter paper.</p><p>For sample solutions nominally containing 0.08 mg/mL of calcium acetate, 5.97 mL of sample stock solution are transferred into a clean 500 mL volumetric flask. This is diluted and made up to the mark with UPW. All solutions are sonicated for 5 minutes before injection.</p><p><strong>CONCLUSION</strong></p><p>According to the USP monograph for calcium acetate capsules [3], the assay for calcium acetate involves determining calcium content using ion chromatography (IC) on a separation column with L76 packing material (here: Metrosep C 6). The validation results met all requirements of the monograph and adhered to the guidelines specified in USP General Chapters &lt;621&gt; Chromatography and &lt;1225&gt; Validation of Compendial Procedures [4,5]. The described IC method is appropriate for quantifying calcium in calcium acetate capsules.</p><h4><strong>5. Thermo Fisher Scientific / </strong><a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/companies/294"><strong>MSACL</strong></a><strong>: </strong><a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/labrulez-bucket-strapi-h3hsga3/po_quantitation_per_polyfluoroalkyl_human_serum_po_en_e61e41c57b.pdf"><strong>Quantitation of a Panel of Per- and Polyfluoroalkyl Substances (PFAS) in Human Serum</strong></a></h4><ul><li>Poster (<a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/companies/294"><strong>MSACL</strong></a>)</li></ul><h5><strong>Abstract</strong></h5><p><strong>Purpose:</strong> Develop an LC-MS analytical method for quantitation of PFAS compound in human serum.</p><p><strong>Methods:</strong> Human serum samples were spiked with 37 PFAS compounds followed by protein precipitation with 4x volume of methanol. The supernatant was analyzed on an LC-MS system equipped with low-PSAF tubing and a delay column to minimize background interferences.</p><p><strong>Results: </strong>LOQs from 0.025 to 2.5 ng/mL were reached for all compounds.</p><h5><strong>Introduction</strong></h5><p>Per- and polyfluoroalkyl substances have been monitored in environmental and industrial samples for some time. Wellness panels are becoming more frequent and quantitating PFAS in biological samples are a part of these panels. Due to the evolving regulations and discoveries of potential health effects with these compounds it important to measure a wide range of PFAS compounds.</p><h5><strong>Conclusions</strong></h5><ul><li>10-minute method for 37 PFAS compounds on the Vanquish UHPLC and TSQ Altis Plus was developed. The method showed good accuracy and precision measurements.</li><li>While this method used simple protein precipitation for sample processing, future work includes developing an SPE method to enhance detection limits.</li></ul>

News from LabRulezLCMS Library - Week 50, 2024

<p><strong>In the week of December 16, the following webinars await you in the field of liquid phase and especially HPLC and LC/MS.</strong></p><blockquote><p><a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/webinars"><strong>👉 DETAILS AND REGISTRATION IN THE WEBINARS SECTION</strong></a></p><p><strong>👉 Search and filter in the largest global database</strong> with over <strong>3 500 GC, LC, MS and spectroscopy webinars</strong>.</p></blockquote><h5><strong>1. SelectScience/Beckman Coulter: Enable seamless, scalable high-throughput proteomics sample prep solutions with open platform liquid handling</strong></h5><div><div><ul><li><div>Mo, 16.12.2024</div></li><li><div><a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/webinar/4540">Registration</a></div></li></ul></div><p>Evosep and Beckman Coulter Life Sciences have partnered to automate sample preparation workflows utilizing the unique analytical properties of the Evotip Pure.</p></div><h5><strong>2.&nbsp;Waters Corporation: Tackling separation challenges with advanced asymmetric field flow fractionation: Future directions in technology</strong></h5><div><div><ul><li><div>Tu, 17.12.2024 17:00 CET</div></li><li><div><a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/webinar/4539">Registration</a></div></li></ul></div><p>We will share the most recent advances in instrumentation and software that bring streamlined workflows and highly reproducible results. Join us and discover the latest innovations in FFF.</p></div><h5><strong>3.&nbsp;Waters Corporation: LG Polymers</strong></h5><div><div><ul><li><div>Th, 19.12.2024 19:00 CET</div></li><li><div><a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/webinar/4170">Registration</a></div></li></ul></div><p>Solving Problems That Matter - a Waters webinar series featuring USP speakers. Episode 11: Complex excipients: LG Polymers.</p></div><h5><strong>4.&nbsp;Shimadzu Corporation: Unlocking EPA Methods 537.1 &amp; 533: Optimize Your PFAS Analysis</strong></h5><div><div><ul><li><div>Th, 19.12.2024 20:00 CET</div></li><li><div><a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/webinar/4463">Registration</a></div></li></ul></div><p>Update your knowledge about the essential factors of PFAS analysis to support the PFAS National Primary Drinking Water Regulation.</p></div>

Webinars LabRulezLCMS Week 51/2024

<p><strong>High-Performance Liquid Chromatography (HPLC)</strong> is a powerful analytical technique, but achieving consistent and reliable results <strong>requires attention to detail and technique</strong>. Here you can find webinars selection from year 2024 focused on <strong>HPLC tips and tricks</strong> to optimize your experiments and troubleshoot common issues:</p><h5><strong>1. MAC-MOD ANALYTICAL: Introducing a Screening Platform for U/HPLC Method Development</strong></h5><div><div><ul><li><div><span style="color:#D92B04;">RECORD</span> | Already taken place Th, 12.12.2024</div></li><li><div><a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/webinar/4533">Registration</a></div></li></ul></div><p>This webinar is the final part in a three-part series called "A Systematic UHPLC/HPLC Method Development Strategy with Complementary Stationary Phases to Maximize Selectivity and Resolution."</p><h5><strong>2.&nbsp;Waters Corporation: Chromatographic System Troubleshooting: A Systematic Approach</strong></h5><div><div><ul><li><div><span style="color:#D92B04;">RECORD</span> | Already taken place We, 4.12.2024</div></li><li><div><a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/webinar/4501">Registration</a></div></li></ul></div><p>In this webinar, a simple and easy to follow protocol for troubleshooting LC systems will be proposed.</p><blockquote><h5><strong>Tip 1: Mobile Phase Preparation</strong></h5><ul><li><strong>Use High-Purity Solvents:</strong> Always use HPLC-grade solvents to prevent contamination and reduce baseline noise.</li><li><strong>Filter and Degas:</strong> Filter the mobile phase using 0.2 µm or 0.45 µm filters and degas it to remove air bubbles that can cause pump and detector issues.</li><li><strong>Proper Mixing:</strong> Ensure solvents are properly mixed when preparing gradients. Use a magnetic stirrer or mix by shaking thoroughly.</li></ul></blockquote></div></div><h5><strong>3.&nbsp;SelectScience/Agilent Technologies: Elevate your purification strategy with best practices for workflow efficiency and scale-up</strong></h5><div><div><ul><li><div><span style="color:#D92B04;">RECORD</span> | Already taken place Th, 14.11.2024</div></li><li><div><a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/webinar/4424">Registration</a></div></li></ul></div><p>Best practices for method optimization to enhance your purification efficiency. You will also learn about scaling up methods and discover practical tips to improve workflow efficiency.</p><h5><strong>4.&nbsp;Phenomenex: LC Pressure 101</strong></h5><div><div><ul><li><div><span style="color:#D92B04;">RECORD</span> | Already taken place Tu, 12.11.2024</div></li><li><div><a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/webinar/4417">Registration</a></div></li></ul></div><p>Familiarize yourself with a standardized protocol for transitioning an LC column from one solvent system to another, ensuring a seamless solvent switch.</p></div></div><h5><strong>5.&nbsp;Phenomenex: How to Choose a Phenyl Phase</strong></h5><div><div><ul><li><div><span style="color:#D92B04;">RECORD</span> | Already taken place Th, 24.10.2024</div></li><li><div><a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/webinar/4393">Registration</a></div></li></ul></div><p>Learn about selectivity benefits of phenyl phases during reversed-phase HPLC. Review mobile-phase optimization when using a phenyl column.</p><blockquote><h5><strong>Tip 2: Column Care</strong></h5><ul><li><strong>Equilibration:</strong> Allow the column to equilibrate with the mobile phase for 10–20 column volumes before starting a run.</li><li><strong>Storage:</strong> Use an appropriate storage solvent (e.g., 100% organic solvent or a mobile phase with preservatives) to prevent microbial growth.</li><li><strong>Flush Regularly:</strong> After using buffer-based mobile phases, flush the column with water followed by an organic solvent like methanol or acetonitrile.</li><li><strong>Avoid Pressure Spikes:</strong> Gradually ramp up the flow rate to protect the column.</li></ul></blockquote><h5><strong>6.&nbsp;Agilent Technologies: USP 621 Decoded</strong></h5><div><div><ul><li><div><span style="color:#D92B04;">RECORD</span> | Already taken place Tu, 8.10.2024</div></li><li><div><a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/webinar/4325">Registration</a></div></li></ul></div><p>Enhance Lab Productivity While Meeting Regulatory Requirements</p></div></div><h5><strong>7.&nbsp;Waters Corporation: HPLC Troubleshooting: A System Monitoring Approach</strong></h5><div><div><ul><li><div><span style="color:#D92B04;">RECORD</span> | Already taken place Tu, 24.9.2024</div></li><li><div><a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/webinar/4279">Registration</a></div></li></ul></div><p>Troubleshooting HPLC systems and chromatographic issues using a structured workflow.</p></div><h5><strong>8.&nbsp;Waters Corporation: Development of Reversed-Phase Methods for the Analysis of Biotherapeutic Synthetic Peptides including GLP-1 Agonists</strong></h5><div><div><ul><li><div><span style="color:#D92B04;">RECORD</span> | Already taken place Tu, 17.9.2024</div></li><li><div><a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/webinar/4287">Registration</a></div></li></ul></div><p>This webinar will review some of the principles and practices to develop robust RP separation methods showing examples using GLP-1 Agonists.</p><blockquote><h5><strong>Tip 3: Sample Preparation</strong></h5><ul><li><strong>Filter Samples:</strong> Use a syringe filter (0.2 µm or 0.45 µm) to remove particulates that can clog the column.</li><li><strong>Match Solvent Strength:</strong> Dissolve samples in a solvent similar to the starting mobile phase composition to avoid peak distortion.</li><li><strong>Use Internal Standards:</strong> These help improve quantitation and compensate for variability.</li></ul></blockquote></div><h5><strong>9.&nbsp;Agilent Technologies: Getting the Most from Your Diode Array Detector: From Selection to Optimization</strong></h5><div><div><ul><li><div><span style="color:#D92B04;">RECORD</span> | Already taken place Th, 29.8.2024</div></li><li><div><a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/webinar/3913">Registration</a></div></li><li><div><a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/labrulez-bucket-strapi-h3hsga3/getting_the_most_from_your_diode_array_detector_aug292024_a46bb2eeed.pdf">Presentation in pdf for download</a></div></li></ul></div><p>we will cover the fundamentals of how your DAD works. From there we will discuss the various options for DAD method optimization.</p></div><h5><strong>10.&nbsp;Agilent Technologies: Decoding LC: Troubleshooting and Maintenance Challenges.</strong></h5><div><div><ul><li><div><span style="color:#D92B04;">RECORD</span> | Already taken place Tu, 23.7.2024</div></li><li><div><a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/webinar/4127">Registration</a></div></li></ul></div><p>This exclusive session is designed specifically to address and help you overcome most pressing maintenance and troubleshooting hurdles in HPLC operation.</p></div><h5><strong>11.&nbsp;Agilent Technologies: Care and Feeding of Your HPLC System: Preventive Maintenance Tips</strong></h5><div><div><ul><li><div><span style="color:#D92B04;">RECORD</span> | Already taken place Tu, 16.7.2024</div></li><li><div><a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/webinar/3899">Registration</a></div></li><li><div><a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/labrulez-bucket-strapi-h3hsga3/care_and_feeding_of_your_hplc_system_july162024_c4c41291fb.pdf">Presentation in pdf for download</a></div></li></ul></div><p>We’ll provide an overview of the LC system, from solvent bottles to detector, with a focus on preventive maintenance and keeping systems running at optimal performance.</p><blockquote><h5><strong>Tip 4: System Maintenance</strong></h5><ul><li><strong>Regularly Clean the System:</strong> Flush the injector, pump, and tubing to prevent salt build-up or contamination.</li><li><strong>Monitor Seals and Filters:</strong> Check and replace worn pump seals, check valves, and inline filters periodically.</li><li><strong>Avoid Overloading:</strong> Use an appropriate sample concentration to prevent column overloading and ensure accurate results.</li></ul></blockquote></div><h5><strong>12.&nbsp;Agilent Technologies: My Chromatography Has Changed: Steps for Effective Troubleshooting</strong></h5><div><div><ul><li><div><span style="color:#D92B04;">RECORD</span> | Already taken place Tu, 18.6.2024</div></li><li><div><a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/webinar/3890">Registration</a></div></li><li><div><a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/labrulez-bucket-strapi-h3hsga3/my_chromatography_has_change_steps_for_effective_troubleshooting_june182024_584cf8c812.pdf">Presentation in pdf for download</a></div></li></ul></div><p>We’ll discuss some of the most common chromatography issues that we see as technical support specialists.</p></div><h5><strong>13.&nbsp;Phenomenex: HPLC Troubleshooting</strong></h5><div><div><ul><li><div><span style="color:#D92B04;">RECORD</span> | Already taken place Tu, 11.6.2024</div></li><li><div><a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/webinar/3587">Registration</a></div></li></ul></div><p>Learn how to prevent and minimize your LC problems before they occur.</p><blockquote><h5><strong>Tip 5: Troubleshooting Common Issues</strong></h5><ul><li><strong>Baseline Noise or Drift</strong><ul><li>Check for air bubbles in the detector or mobile phase.</li><li>Ensure the mobile phase is fresh and fully degassed.</li><li>Inspect the detector lamp for aging or instability.</li></ul></li><li><strong>Peak Tailoring</strong><ul><li>Use columns with appropriate stationary phases and well-maintained packing.</li><li>Ensure no column voids are present.</li><li>Adjust mobile phase pH for ionizable compounds.</li></ul></li><li><strong>Low Resolution or Overlapping Peaks</strong><ul><li>Optimize flow rate, gradient, or mobile phase composition.</li><li>Use a column with smaller particle size or longer length.</li></ul></li><li><strong>Retention Time Shifts</strong><ul><li>Confirm consistent mobile phase composition and column temperature.</li><li>Check for leaks in the system or improper mixing in gradient systems.</li></ul></li></ul></blockquote></div><h5><strong>14.&nbsp;Agilent Technologies: Decoding LC: Navigating Workflow Challenges</strong></h5><div><div><ul><li><div><span style="color:#D92B04;">RECORD</span> | Already taken place We, 15.5.2024</div></li><li><div><a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/webinar/3824">Registration</a></div></li></ul></div><p>Get actionable insights, practical tips, and proven strategies to not only overcome obstacles but to significantly enhance your workflow's efficiency.</p></div><h5><strong>15.&nbsp;Agilent Technologies: Method Makeover: Updating Older HPLC Methods</strong></h5><div><div><ul><li><div><span style="color:#D92B04;">RECORD</span> | Already taken place Tu, 16.4.2024</div></li><li><div><a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/webinar/3000">Registration</a></div></li><li><div><a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/labrulez-bucket-strapi-h3hsga3/method_makeover_updating_older_hplc_methods_april162024_bca4658cbc.pdf">Presentation in pdf for download</a></div></li></ul></div><p>Modernizing a legacy method can improve accuracy, ruggedness, and sample throughput.</p><blockquote><h5><strong>Tip 6: Optimize Method Development</strong></h5><ul><li><strong>Gradient Optimization:</strong> Start with a wide gradient range to identify peak separation potential, then narrow it for resolution.</li><li><strong>pH Adjustment:</strong> Fine-tune mobile phase pH for ionizable analytes to maximize separation.</li><li><strong>Temperature Control:</strong> Maintain consistent column temperature to improve reproducibility and resolution.</li></ul></blockquote></div><h5><strong>16.&nbsp;Thermo Fisher Scientific: Back to basics: Revealing the strength of HPLC-CAD methods</strong></h5><div><div><ul><li><div><span style="color:#D92B04;">RECORD</span> | Already taken place Th, 4.4.2024</div></li><li><div><a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/webinar/3689">Registration</a></div></li></ul></div><p>In this live webinar, we will highlight some distinctions between CAD and other UV-absorbance detection, such as the non-linear response to analyte concentration, and sensitivity to mobile phase impurities.</p></div><h5><strong>17.&nbsp;Phenomenex: Knowing your Analyte Properties to Optimize Analysis</strong></h5><div><div><ul><li><div><span style="color:#D92B04;">RECORD</span> | Already taken place Tu, 13.2.2024</div></li><li><div><a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/webinar/3476">Registration</a></div></li></ul></div><p>Understand the importance of knowing your analyte and how they influence analysis and optimization.</p></div><h5><strong>18.&nbsp;Phenomenex: How To Start Up and Shut Down LC and LC-MS Instruments</strong></h5><div><div><ul><li><div><span style="color:#D92B04;">RECORD</span> | Already taken place We, 31.1.2024</div></li><li><div><a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/webinar/3383">Registration</a></div></li></ul></div><p>Getting your system ready. LC System Start Up and Shut Down. LC-MS System Start Up and Shut Down. On and Off System Column Storage.</p><blockquote><h5><strong>7. General Best Practices</strong></h5><ul><li><strong>Log System Conditions:</strong> Record mobile phase, flow rate, column temperature, and pressure for reproducibility.</li><li><strong>Keep Spare Parts:</strong> Maintain spare seals, filters, and lamps to minimize downtime.</li><li><strong>Training:</strong> Regularly train operators on best practices and maintenance.</li></ul></blockquote></div>

HPLC Tips & Tricks webinars selection in 2024

<p><strong>LC/MS (Liquid Chromatography-Mass Spectrometry) </strong>is a powerful analytical tool that combines the separation capabilities of <strong>liquid chromatography</strong> with the detection and identification strengths of <strong>mass spectrometry</strong>. Here are some tips and tricks to optimize your LC/MS analysis:</p><blockquote><p><strong>See also the first part: </strong><a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/article/4850"><strong>HPLC Tips &amp; Tricks webinars selection in 2024</strong></a></p></blockquote><h5><strong>1.&nbsp;Shimadzu Corporation: Unlocking EPA Methods 537.1 &amp; 533: Optimize Your PFAS Analysis</strong></h5><div><div><ul><li><div><span style="color:#D92B04;">RECORD</span> | Already taken place Th, 19.12.2024</div></li><li><div><a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/webinar/4463">Registration</a></div></li></ul></div><p>Update your knowledge about the essential factors of PFAS analysis to support the PFAS National Primary Drinking Water Regulation.</p></div><h5><strong>2.&nbsp;SelectScience/Agilent Technologies: Metabolite imaging signals the next frontier in spatial biology</strong></h5><div><div><ul><li><div><span style="color:#D92B04;">RECORD</span> | Already taken place Tu, 12.11.2024</div></li><li><div><a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/webinar/4419">Registration</a></div></li></ul></div><p>An overview of the various technologies available for MSI, covering different sources and mass spectrometers, from home-built solutions to commercial options.</p><blockquote><h5><strong>Tip 1: Preparation and Setup</strong></h5><ul><li><strong>Mobile Phase Considerations</strong>: Use LC/MS-grade solvents to minimize contamination. Avoid non-volatile salts in the mobile phase as they can interfere with ionization and clog the MS source. If buffers are required, use volatile options (e.g., ammonium acetate or ammonium formate).</li><li><strong>Sample Preparation</strong>: Avoid high concentrations of salts, detergents, or other contaminants that suppress ionization.</li><li><strong>Column Selection</strong>: Ensure the column is MS-compatible and can handle your solvent composition.</li></ul></blockquote></div><h5><strong>3.&nbsp;Agilent Technologies: Troubleshoot Your Misbehaving Triple Quadrupole LC/MS</strong></h5><div><div><ul><li><div><span style="color:#D92B04;">RECORD</span> | Already taken place Fr, 25.10.2024</div></li><li><div><a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/webinar/4394">Registration</a></div></li></ul></div><p>Are you ready to take your LC-TQ troubleshooting skills to the next level?</p></div><h5><strong>4.&nbsp;Waters Corporation: Development of Reversed-Phase Methods for the Analysis of Biotherapeutic Synthetic Peptides including GLP-1 Agonists</strong></h5><div><div><ul><li><div><span style="color:#D92B04;">RECORD</span> | Already taken place Tu, 17.9.2024</div></li><li><div><a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/webinar/4287">Registration</a></div></li></ul></div><p>This webinar will review some of the principles and practices to develop robust RP separation methods showing examples using GLP-1 Agonists.</p><blockquote><h5><strong>Tip 2: System Maintenance</strong></h5><ul><li><strong>Tuning and Calibration</strong>: Regularly calibrate the MS to maintain mass accuracy. Optimize source parameters (e.g., voltage, temperature, gas flows) for your compound class.</li><li><strong>Prevent Contamination</strong>: Clean the ion source periodically to remove salt or matrix deposits. Regularly clean or replace parts like the cone, capillary, or skimmer.</li></ul></blockquote></div><h5><strong>5.&nbsp;Agilent Technologies: Reaching Ultra-Trace PFAS Detection Limits by Overcoming Solvent Effects at High Injection Volumes</strong></h5><div><div><ul><li><div><span style="color:#D92B04;">RECORD</span> | Already taken place We, 21.8.2024</div></li><li><div><a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/webinar/4165">Registration</a></div></li></ul></div><p>High concentration factor in sample preparation with the use of Feed Injection for improved chromatographic performance at high injection volumes and with LC/MS allows lowest possible PFAS detection limits.</p><h5><strong>6.&nbsp;Separation Science/SCIEX: PFAS Analysis in Food: A Robustness Study in Sensitivity and Stability</strong></h5><div><div><ul><li><div><span style="color:#D92B04;">RECORD</span> | Already taken place We, 31.7.2024</div></li><li><div><a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/webinar/4112">Registration</a></div></li></ul></div><p>Learn how cutting-edge mass spec technology can co-exist with stability and robustness. It doesn’t have to be a compromise.</p></div></div><h5><strong>7.&nbsp;SCIEX: Troubleshooting at the source</strong></h5><div><div><ul><li><div><span style="color:#D92B04;">RECORD</span> | Already taken place Tu, 2.7.2024</div></li><li><div><a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/webinar/3855">Registration</a></div></li></ul></div><p>Elevate your analytical expertise with advanced techniques and workflows.</p><blockquote><h5><strong>Tip 3: Operation</strong></h5><ul><li><strong>Ionization Source Optimization</strong>: Select the appropriate ionization mode (ESI for polar compounds, APCI for less polar). Adjust nebulizer and desolvation gas flows for optimal droplet formation.</li><li><strong>Gradient Design</strong>: Use a suitable gradient profile for efficient separation without excessively long run times. Gradually increase organic content to avoid sudden pressure changes or retention issues.</li><li><strong>Flow Rate Management</strong>: Match LC flow rates to the MS inlet specifications (typically 0.2 – 1.0 mL/min). Use a flow splitter if necessary to prevent overloading the MS.</li></ul></blockquote></div><h5><strong>8.&nbsp;SCIEX: It's a TRAP! Getting the most out of your QTRAP system</strong></h5><div><div><ul><li><div><span style="color:#D92B04;">RECORD</span> | Already taken place Tu, 18.6.2024</div></li><li><div><a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/webinar/3854">Registration</a></div></li></ul></div><p>Elevate your analytical expertise with advanced techniques and workflows.</p></div><h5><strong>9.&nbsp;SCIEX: Understanding capillary electrophoresis</strong></h5><div><div><ul><li><div><span style="color:#D92B04;">RECORD</span> | Already taken place Tu, 18.6.2024</div></li><li><div><a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/webinar/3891">Registration</a></div></li></ul></div><p>In this series, you will learn the fundamentals of the classical applications of CE.</p><blockquote><h5><strong>Tip 4: Troubleshooting</strong></h5><ul><li><strong>Signal Problems</strong>:<ul><li><strong>Low signal:</strong> Check ionization efficiency, tuning parameters, and solvent purity.</li><li><strong>Signal instability:</strong> Verify pump performance, solvent degassing, and source cleanliness.</li></ul></li><li><strong>Peak Issues</strong>:<ul><li><strong>Broad or tailing peaks:</strong> Optimize column temperature, flow rate, and injection volume.</li><li><strong>Missing peaks:</strong> Verify sample preparation, gradient, and detection settings.</li></ul></li><li><strong>Mass Accuracy</strong>:<ul><li>Use lock mass or internal calibration to correct drift.</li><li>Monitor and adjust for environmental factors like temperature and humidity.</li></ul></li></ul></blockquote></div><h5><strong>10.&nbsp;Thermo Fisher Scientific: Sample Preparation for Clinical Liquid Chromatography-Mass Spectrometry Assays</strong></h5><div><div><ul><li><div><span style="color:#D92B04;">RECORD</span> | Already taken place Th, 13.6.2024</div></li><li><div><a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/webinar/3978">Registration</a></div></li></ul></div><p>This webinar encompasses various sample preparation options for liquid chromatography-mass spectrometry (LC-MS) assays.</p></div><h5><strong>11.&nbsp;SCIEX: Excel strategies for mass spectrometry data analysis</strong></h5><div><div><ul><li><div><span style="color:#D92B04;">RECORD</span> | Already taken place Th, 16.5.2024</div></li><li><div><a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/webinar/3709">Registration</a></div></li></ul></div><p>Take an in-depth look at when and how to use Excel to increase your productivity.</p><blockquote><h5><strong>Tip 5: Data Analysis</strong></h5><ul><li><strong>Baseline Noise</strong>: Minimize noise by using clean solvents and optimizing the source parameters. Apply appropriate smoothing or baseline correction during data processing.</li><li><strong>Quantitation Tips</strong>: Use stable isotope-labeled internal standards when possible. Ensure the linearity of calibration curves across the concentration range.</li><li><strong>Fragmentation Optimization</strong>: Adjust collision energy for clear and interpretable fragmentation patterns. Use MS/MS or MRM modes for increased specificity.</li></ul></blockquote></div><h5><strong>12.&nbsp;SCIEX: Troubleshooting at the source (LC/MS)</strong></h5><div><div><ul><li><div><span style="color:#D92B04;">RECORD</span> | Already taken place Th, 18.4.2024</div></li><li><div><a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/webinar/3707">Registration</a></div></li></ul></div><p>Acquire the knowledge needed to optimize source parameters and identify and resolve ionization-related problems.</p></div><h5><strong>13.&nbsp;SCIEX: It's a TRAP! Getting the most out of your QTRAP system</strong></h5><div><div><ul><li><div><span style="color:#D92B04;">RECORD</span> | Already taken place Th, 4.4.2024</div></li><li><div><a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/webinar/3706">Registration</a></div></li></ul></div><p>Learn when to utilize QTRAP functionality to create more selective and sensitive methods.</p></div><h5><strong>14.&nbsp;Waters Corporation: Empower Tips: Ask Me Anything About Minimizing Risk in Your Analysis with Empower Software and Mass Detection</strong></h5><div><div><ul><li><div><span style="color:#D92B04;">RECORD</span> | Already taken place We, 13.3.2024</div></li><li><div><a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/webinar/3664">Registration</a></div></li></ul></div><p>Collecting UV and MS spectra contemporaneously helps to assure the absence of contaminants with similar chemical properties to your main compounds of interest.</p></div><h5><strong>15.&nbsp;Phenomenex: How To Start Up and Shut Down LC and LC-MS Instruments</strong></h5><div><div><ul><li><div><span style="color:#D92B04;">RECORD</span> | Already taken place We, 31.1.2024</div></li><li><div><a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/webinar/3383">Registration</a></div></li></ul></div><p>Getting your system ready. LC System Start Up and Shut Down. LC-MS System Start Up and Shut Down. On and Off System Column Storage.</p><blockquote><h5><strong>Tip 6: General Best Practices</strong></h5><ul><li><strong>Documentation</strong>: Maintain detailed logs of instrument settings, mobile phase compositions, and sample information. Record and save method parameters and calibration data.</li><li><strong>Preventive Maintenance</strong>: Regularly clean or replace worn parts (e.g., seals, capillaries, and detector components). Follow the manufacturer’s maintenance schedule to prolong system life.</li><li><strong>Training and Knowledge</strong>: Stay updated on new techniques, applications, and troubleshooting strategies. Collaborate with colleagues and attend workshops or webinars to enhance your skills.</li></ul></blockquote><p><strong>By applying these tips and tricks, you can improve the reliability, sensitivity, and accuracy of your LC/MS analyses while reducing downtime and troubleshooting efforts.</strong></p><ul><li><p class="cke-user_style-important"><a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/webinars?tags=21-15"><strong>Explore all LCMS webinars focusing on LC/MS, Tips &amp; Tricks in the LabRulez LCMS database.</strong></a></p></li><li><p class="cke-user_style-important"><a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/library?instrumentationItems=31&amp;search=Troubleshooting"><strong>Explore all litarature docused on LC/MS, Tips &amp; Tricks and Troubleshooting in the LabRulez LCMS library.</strong></a></p></li></ul></div>

LC/MS Tips & Tricks webinars selection in 2024

<p><strong>Dear LabRulez portal users, customers and friends,</strong></p><p>As the year draws to a close, we would like to thank you for your trust, support and cooperation in helping us to make 2024 another successful year and to confirm our position in the complex online world.</p><ul><li><strong>Number of users: + 311 500</strong></li><li><strong>Page views: + 866 700</strong></li><li><strong>Google views: + 21 338 000</strong></li><li><strong>Number of opened PDFs: + 1 000 000</strong></li></ul><p>This year has been special for us:</p><ul><li><strong>We have expanded the amount of content and companies/institutions on our Czech and Global ENG portals:</strong><ul><li><div><strong>+ 1 100 </strong><a style="text-decoration:none!important;" target="_blank" rel="noopener noreferrer" href="https://labrulezlinks.seurl.cz/email-audience/link?acc=123254b4-60ba-11eb-abc8-ac1f6bc402ab&amp;stat=fc61f5bbc6404c10b69e0526dce56695&amp;link=365f29c05f59f09b27a7d9401c64cdb8" 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institutions.</strong></p><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Lab_Rulez_Servisni_centrum_a_laborator_Lab_Rulez_pro_udrzbu_a_servis_zanovnichstarsich_BAZAR_instrumentu_18aa8af0de.jpg" alt="LabRulez: LabRulez Service Centre and Laboratory for maintenance and servicing of older BAZAAR instruments" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Lab_Rulez_Servisni_centrum_a_laborator_Lab_Rulez_pro_udrzbu_a_servis_zanovnichstarsich_BAZAR_instrumentu_18aa8af0de.jpg 245w," sizes="100vw" width="4032" height="2268"></p><h3><strong>What are our plans for 2025?</strong></h3><p>We plan to continue innovating and we are preparing several new sections for you:</p><ul><li><strong>The main new feature will be an open academic section</strong> (analytical Wikipedia), which will bring you <strong>theoretical and practical quality/proof educational content, service tips &amp; tricks and discussion forum</strong>.</li><li>We will also expand <strong>the BAZAAR section</strong> and our service facilities.</li></ul><p>Thank you for being part of our journey. We look forward to more successes together and what 2025 will bring.</p><p>May the year 2025 be full of fulfilled wishes and successes for you!</p><p>With friendly regards</p><p><strong>Ivo Novotný</strong><br>(CEO &amp; Founder, LabRulez s.r.o.)</p><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Lab_Rulez_Ivo_Novotny_CEO_and_Founder_Lab_Rulez_s_r_o_af1d7cc897.jpg" alt="LabRulez: Ivo Novotný  CEO &amp; Founder, LabRulez s.r.o.)" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Lab_Rulez_Ivo_Novotny_CEO_and_Founder_Lab_Rulez_s_r_o_af1d7cc897.jpg 245w," sizes="100vw" width="1713" height="963"></p>

LabRulez portals in 2024 and our plans for 2025

<p><strong>In the week of January 6, the following webinars await you in the field of liquid phase and especially HPLC and LC/MS.</strong></p><p><strong>We bring you also a selection of the TOP 10 most requested webinars in the field of liquid phase and especially HPLC and LC/MS on LabRuleLCMS portals in 2024.</strong></p><blockquote><p><a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/webinars"><strong>👉 DETAILS AND REGISTRATION IN THE WEBINARS SECTION</strong></a></p><p><strong>👉 Search and filter in the largest global database</strong> with over <strong>3 500 GC, LC, MS and spectroscopy webinars</strong>.</p></blockquote><h4><strong>Week 2/2025</strong></h4><h5><strong>1.&nbsp;Waters Corporation: Advancing GLP-1 analog drug development using a variety of light scattering techniques​</strong></h5><div><div><ul><li><div>We, 8.1.2025 05:00 CET</div></li><li><div><a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/webinar/4543">Registration</a></div></li></ul></div><p>This webinar will focus on the basics of light scattering and its data analysis, as well as present case studies that highlight how light scattering can be utilized in GLP-1 analogs drug development.</p></div><h5><strong>2. &nbsp;Shimadzu Corporation: Accelerating PFAS Detection in Complex Matrices by EPA Method 1633</strong></h5><div><div><ul><li><div>Th, 9.1.2025 20:00 CET</div></li><li><div><a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/webinar/4464">Registration</a></div></li></ul></div><p>Learn how to get excellent LC-MS/MS performance for simplified PFAS analysis within the flexibility afforded by EPA method 1633.</p></div><h4><strong>TOP 10 in 2024</strong></h4><h5><strong>1.&nbsp;Separation Science/Merck: Validation of Parameters for Karl Fischer Titration</strong></h5><div><div><ul><li><div><span style="color:#D92B04;">RECORD</span> | Already taken place We, 21.6.2023</div></li><li><div><a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/webinar/2612">Registration</a></div></li></ul></div><p>The objective of this session is to present the requirements for a titration process.</p></div><h5><strong>2.&nbsp;Waters Corporation: Empower Tips Special Edition: Ask Me Anything About Our Newest Empower Release - Empower 3.8.0</strong></h5><div><div><ul><li><div><span style="color:#D92B04;">RECORD</span> | Already taken place Tu, 11.4.2023</div></li><li><div><a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/webinar/2488">Registration</a></div></li></ul></div><p>Join us for a live discussion as we explore Empower 3.8.0. As always, don’t forget to bring your questions about Empower Software.</p></div><h5><strong>3.&nbsp;Waters Corporation: Empower Tips: Ask Me Anything About Calculating Signal to Noise Ratio</strong></h5><div><div><ul><li><div><span style="color:#D92B04;">RECORD</span> | Already taken place Tu, 8.8.2023</div></li><li><div><a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/webinar/2892">Registration</a></div></li></ul></div><p>The calculation of signal to noise ratio is a critical parameter during method development, method validation and routine monitoring the performance of a chromatographic method.</p></div><h5><strong>4.&nbsp;Thermo Fisher Scientific: Quantitation of Poloxamer P188 in Biopharmaceutical Formulations Using Charged Aerosol Detection</strong></h5><div><div><ul><li><div><span style="color:#D92B04;">RECORD</span> | Already taken place Tu, 11.7.2023</div></li><li><div><a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/webinar/2750">Registration</a></div></li></ul></div><p>In his presentation, he will share his experience of the development and validation strategies of a robust method for the quantitation of poloxamer P188 in biopharmaceuticals.</p></div><h5><strong>5.&nbsp;Agilent Technologies: Quant Made Easy: Building a Calibration Curve with Agilent MassHunter Quantitative Analysis</strong></h5><div><div><ul><li><div><span style="color:#D92B04;">RECORD</span> | Already taken place We, 29.3.2023</div></li><li><div><a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/webinar/2504">Registration</a></div></li></ul></div><p>In MassHunter Quantitative software, the calibration curve basics will be reviewed and a convenient and quick method for importing the calibration levels from CSV file will be featured.</p></div><h5><strong>6.&nbsp;Agilent Technologies: OpenLab CDS UV Spectral &amp; Peak Purity - How to Perform 3D data analysis for compound &amp; UV purity confirmation</strong></h5><div><div><ul><li><div><span style="color:#D92B04;">RECORD</span> | Already taken place Fr, 8.4.2022</div></li><li><div><a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/webinar/1740">Registration</a></div></li></ul></div><p>In this session, we will talk about 3D data analysis features available in OpenLab CDS.</p></div><h5><strong>7.&nbsp;Agilent Technologies: OpenLab CDS System Suitability &amp; Calibration - How to perform System suitability &amp; Calibration</strong></h5><div><div><ul><li><div><span style="color:#D92B04;">RECORD</span> | Already taken place Fr, 1.4.2022</div></li><li><div><a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/webinar/1739">Registration</a></div></li></ul></div><p>In this session you will learn how to setup the method to calculate system suitability parameters like Signal to noise ratio, Resolution, theoretical plates, etc from different pharmacopeia like USP, JP &amp; EP.</p></div><h5><strong>8.&nbsp;Agilent Technologies: OpenLab CDS - Archive, Backup/Restore and server maintenance essentials.</strong></h5><div><div><ul><li><div><span style="color:#D92B04;">RECORD</span> | Already taken place Fr, 29.4.2022</div></li><li><div><a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/webinar/1743">Registration</a></div></li></ul></div><p>In this session, we will talk about how to perform Backup / restore functionalities and best practise to maintain OpenLab CDS server.</p></div><h5><strong>9.&nbsp;Agilent Technologies: Basics of LC/QTOF Operation - Part 1</strong></h5><div><div><ul><li><div><span style="color:#D92B04;">RECORD</span> | Already taken place Tu, 15.6.2021</div></li><li><div><a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/webinar/970">Registration</a></div></li></ul></div><p>The first part will focus on the proper setup of the QTOF and LC system, a review on how to properly make up fresh tuning solution, and an explanation of how to properly tune and calibrate the QTOF system.</p></div><h5><strong>10.&nbsp;Thermo Fisher Scientific: Compound Discoverer software workshops (General Compound Discoverer software training)</strong></h5><div><div><ul><li><div><span style="color:#D92B04;">RECORD</span> | Already taken place We, 27.9.2023</div></li><li><div><a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/webinar/2936">Registration</a></div></li></ul></div><p>The workshops are designed to bring beginners up-to-speed with best practices as well as allow advanced users to interact with software experts and enquire how to get the most out of their data.</p></div>

Webinars LabRulezLCMS Week 2/2025 + TOP 10 in 2024

Bioinert UHPLC system improves sensitivity and peak shapes for ionic metabolites

<p><strong>Our Library never stops expanding. What are the most recent contributions to LabRulezLCMS Library in the week of 6th January 2025? Check out new documents from the field of liquid phase, especially HPLC and LC/MS techniques!</strong></p><blockquote><p><span style="font-size:20px;">👉 </span><a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/library"><span style="font-size:20px;"><strong>SEARCH THE LARGEST REPOSITORY OF DOCUMENTS ABOUT LCMS AND RELATED TECHNIQUES</strong></span></a></p></blockquote><blockquote><p><strong>👉 Need info about different analytical techniques?</strong> Peek into <a target="_blank" rel="noopener noreferrer" href="https://gcms.labrulez.com/library"><strong>LabRulezGCMS</strong></a> or <a target="_blank" rel="noopener noreferrer" href="https://icpms.labrulez.com/library"><strong>LabRulezICPMS</strong></a> libraries.</p></blockquote><p>This week we bring you applications by <strong>Shimadzu</strong>,<strong> Metrohm</strong>, and<strong> &nbsp;Waters Corporation</strong>, a presentation by <strong>Agilent Technologies</strong>, and a poster from <strong>MSACL </strong>by <strong>Thermo Fisher Scientific</strong>!</p><h4><strong>1. ALS Europe: Environmental Challenges of Pharmaceuticals: Persistence and Accumulation in Ecosystems</strong></h4><ul><li>Technical note</li><li><a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/labrulez-bucket-strapi-h3hsga3/enviromail_21_europe_environmental_challenges_of_pharmaceuticals_nov2024_ad8337748c.pdf"><strong>Full PDF for download</strong></a></li></ul><p>The drugs are used extensively not only in human and veterinary medicine but are also widely found in livestock feed. Because pharmaceuticals are designed to be effective even at low concentrations, these specific contaminants can exist in the environment at extremely low levels. As with pesticides, these compounds can accumulate in different parts of the ecosystem. Since conventional wastewater treatment plants cannot adequately remove these substances, they are released into the environment, including surface and groundwater, and consequently into drinking water sources.</p><h5><strong>Pharmaceuticals as "New" Pesticides...</strong></h5><p>Pharmaceuticals represent a broad and chemically diverse group of substances that is continuously expanding and is characterised by a wide range of clinical effects and extensive use, often leading to overuse, in both human and veterinary medicine. From an environmental perspective, they are classified as organic micropollutants due to their presence in water at low concentrations (ranging from ng/L to μg/L). Both the public and experts are concerned not only about their negative impacts on natural ecosystems but also about their presence in drinking water and the related effects on human health. Their contribution to the emergence of antimicrobial resistance (AMR) is also significant, as it greatly complicates the treatment of infectious diseases and represents a major challenge for current and future medicine.</p><p>Drugs can enter the environment through various pathways. One of the primary sources of contamination is wastewater from households and health and social care facilities, which contains human urine and faeces, as well as unused or expired pharmaceuticals. Another significant source is wastewater from the pharmaceutical industry. Consequently, wastewater treatment plants (WWTPs) themselves become sources of environmental contamination, as they are unable to effectively remove pharmaceuticals, allowing them to be transported further into the environment [1].</p><p><strong>ALS Laboratories have developed and accredited a multiresidue methods for the determination of more than 100 different pharmaceuticals and their metabolites in various types of water. All of our analytical methods use the LC/MS/MS technique for analytes determination, which provides high sensitivity, selectivity and precision of measurement and allows the determination of target compounds at the very low limits required for residue analysis.</strong></p><h4><strong>2. Thermo Fisher Scientific:&nbsp;Orbitrap Astral MS Literature List: Rethink what is possible</strong></h4><ul><li>Guide</li><li><a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/labrulez-bucket-strapi-h3hsga3/eb003337_ms_orbitrap_astral_litlist_eb003337_na_en_454d9ad02c.pdf"><strong>Full PDF for download</strong></a></li></ul><p><strong>Bacterial Proteomics, Drug Resistance, Crosslinking-MS, Metaproteomics, Data Analysis, Mamalian Development, Oxidation, Plant Proteomics, Phosphoproteomics, Plasma Proteomics, Single-Cell Proteomics, Reproducibility, Spatial Proteomics, Salivary Proteome, Technology, Tissue Proteomics, Ubiquitylation, Vaccine Research, Whole Proteome</strong></p><h4><strong>3. Agilent Technologies: Easy and Fast Automation of Sample Preparation</strong></h4><ul><li>Application</li><li><a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/labrulez-bucket-strapi-h3hsga3/te_injector_program_sample_preparation_hplc_5994_7703en_agilent_05f73923a6.pdf"><strong>Full PDF for download</strong></a></li></ul><p><strong>Sample preparation using injector workflows with the Agilent 1260 Infinity III Multisampler and Vialsampler.</strong></p><p>The demand for automated workflows is increasing as the number of samples in the lab continues to grow, but implementing automation in the lab environment is difficult, time-consuming, and costly. This technical overview describes an alternative method for implementing an automation method quickly and easily for sample preparation. This includes derivatization of amino acid samples, sample dilution, and preparation of calibration standards using an Agilent 1260 Infinity III Multisampler or Agilent 1260 Infinity III Vialsampler in combination with injector workflows. The results obtained were excellent in terms of precision, repeatability, and sensitivity, and are independent of the operator's skills.</p><h5><strong>The 1260 Infinity III LC comprised the following modules:</strong></h5><ul><li>Agilent 1260 Infinity III Binary Pump (G7112B)</li><li>Either: Agilent 1260 Infinity III Vialsampler (G7129A) with Integrated Column Compartment (G7130A), 100 μL analytical head and 100 μL sample loop (default setup)</li><li>Or: Agilent 1260 Infinity III Multisampler (G7167A) with 100 μL analytical head and 100 μL sample loop (default setup) and additional Multicolumn Thermostat (G7116A)</li><li>Agilent 1260 Infinity III Variable Wavelength Detector (VWD) (G7114A), equipped with a 10 mm standard flow cell (14 μL, option #018)</li><li>Agilent 1260 Infinity III Fluorescence Detector Spectra (FLD) (G7121B), equipped with an 8 μL FLD cell (G1321-60005)</li></ul><h5><strong>Agilent OpenLab CDS version 2.8 or later</strong></h5><h5><strong>Columns</strong></h5><ul><li>Agilent InfinityLab Poroshell 120 EC-C18, 2.1 × 50 mm, 2.7 μm (part number 699975-902)</li><li>Agilent AdvanceBio AAA LC column, 3.0 × 100 mm, 2.7 μm (part number 695975-322) with Agilent AdvanceBio AAA guard column, 3.0 × 5 mm, 2.7 μm (part number 823750-946)</li></ul><p>Injector workflows, in combination with the Agilent 1260 Infinity III Multisampler or Agilent 1260 Infinity III Vialsampler enable automation of certain sample preparation methods such as derivatization of amino acids, sample dilution, and preparation of calibration standards with excellent precision, repeatability, and sensitivity. The injector workflow differs from the conventional injector program in its ability to set up methods faster and easier without error, saving time and cost.</p><h4><strong>4. Waters Corporation:&nbsp;Evaluation of Modern and Legacy HPLC Systems for the Size-Exclusion Analysis of Aggregation and Fragmentation in a Monoclonal Antibody Drug</strong></h4><ul><li>Application</li><li><a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/labrulez-bucket-strapi-h3hsga3/720008614_en_f03ac5dc38.pdf"><strong>Full PDF for download</strong></a></li></ul><p>Size-exclusion chromatography is a widely used characterization technique for the analysis of aggregation and fragmentation of monoclonal antibody drugs. Traditional high-performance liquid chromatography systems have a large amount of extra-column dispersion which makes size-exclusion data analysis difficult due to band broadening and increased peak tailing. Additionally, biologic analytes may experience non-specific adsorption throughout the chromatographic flow path, further increasing the effects of peak tailing. The Alliance™ iS Bio HPLC System addresses both concerns by offering lower system dispersion than legacy HPLC systems alongside a bio-inert flow path employing MaxPeak™ High-Performance Surfaces Technology.</p><p>In this work an Alliance iS Bio HPLC System, an Alliance e2695, and a competitive Bio-inert HPLC system are compared using a size-exclusion method for analysis of monoclonal antibody aggregation and fragmentation. Dispersion on each system is determined and the Alliance iS Bio HPLC is shown to have the lowest 4σ dispersion. This translates to lower peak tailing, tighter peak width, and a more accurate measurement of low- molecular weight species of infliximab due to lower monomer peak tailing and better monomer resolution.</p><h5><strong>Benefits</strong></h5><ul><li>Biocompatible system for use in salty bio-applications</li><li>MaxPeak HPS for reduced off-target analyte interaction results in less peak tailing</li><li>Low system dispersion results in better fragmentation determination</li></ul><h4><strong>5. Shimadzu: Rapid Screening of 501 Emerging Contaminants in Water Using Polarity-Extended Liquid Chromatography-Mass Spectrometry</strong></h4><ul><li>Application</li><li><a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/labrulez-bucket-strapi-h3hsga3/an_03_lcmsms_919_en_1790a25e03.pdf"><strong>Full PDF for download</strong></a></li></ul><h5><strong>User Benefits</strong></h5><ul><li>Polarity-Extended Liquid Chromatography combines hydrophilic interaction chromatography and reversed-phase chromatography to achieve simultaneous analysis of polar and weakly polar compounds.</li><li>This method can detect 501 emerging contaminants, offering a broad scope in terms of both quantity and variety.</li><li>The water sample is concentrated using freeze-drying method, effectively recovering both polar and non-polar compounds are recovered well.</li></ul><h5><strong>Introduction</strong></h5><p>In recent years, water pollution has become increasingly serious, and the problem of emerging pollutants in water has attracted much attention. Traditional analytical methods for emerging pollutants mainly focus on 1-2 categories of emerging pollutants and pay less attention to strongly polar pollutants. The Polarity-Extended Liquid Chromatography, through patented technologies such as polarity splitting, online dilution, and dual gradients, combines a Hilic column (first dimension) and a C18 column (second dimension), which can broaden the polarity analysis range of compounds and achieve simultaneous analysis of polar and weakly polar compounds. Fig. 1 shows the Polarity-Extended Liquid Chromatography-Mass Spectrometry System.</p><p>In this paper, a method for rapid screening of 501 emerging pollutants was established by using Shimadzu's Polarity-Extended Liquid Chromatography-Mass Spectrometry System. This method solves the problem of simultaneous analysis of strong and weak polar pollutants.</p><h5><strong>Conclusion</strong></h5><p>In this study, a method for detecting emerging pollutants in water by combining the Polarity-Extended Liquid Chromatography and LCMS - 8050 series coupling system was established. This method combines hydrophilic interaction chromatography and reversed - phase chromatography to achieve the simultaneous separation of polar and non - polar compounds with a single injection. The detection of 501 emerging pollutants was achieved within 28 minutes. The linearity and repeatability of the method meet the quantitative requirements. After pretreatment operations such as freeze - drying, re-dissolution, and nitrogen blowing of the samples, the recovery rates for most compounds were satisfactory. This method has been successfully used for the rapid screening of new pollutants in tap water and sewage. The combination of Polarity-Extended Liquid Chromatography and mass spectrometry enables the one-injection analysis of compounds with different polarities without significantly increasing analysis time, making it suitable for the rapid screening of emerging pollutants in water.</p><h4><strong>6. Metrohm:&nbsp;Installation Instruction for ProfIC Vario 7 Anion</strong></h4><ul><li>Manual</li><li><a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/labrulez-bucket-strapi-h3hsga3/AB_379_2_1d0cb4a2d3.pdf"><strong>Full PDF for download</strong></a></li></ul><p>The ProfIC Vario 7 Anion is a Professional IC Vario system with Inline Dilution and Inline Dialysis for the fully automatic determination of anions with sequential suppression in very high concentrations in matrices containing emulsions. This system enables users to dilute the sample and eliminate droplets of fat or organic solvents or particles prior to the analysis.</p>

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News from LabRulezLCMS Library - Week 2, 2025

Bioinert UHPLC system improves sensitivity and peak shapes for ionic metabolites

Webinars LabRulezLCMS Week 2/2025 + TOP 10 in 2024

LabRulez portals in 2024 and our plans for 2025

LC/MS Tips & Tricks webinars selection in 2024

HPLC Tips & Tricks webinars selection in 2024

News from LabRulezLCMS Library - Week 50, 2024

Metabolomics and sensory evaluation of white asparagus ingredients in instant soups unveil important (off-)flavours

Webinars LabRulezLCMS Week 51/2024

News from LabRulezLCMS Library - Week 49, 2024

Upcoming events

Thermo Fisher Scientific Liquid Chromatography Solutions Tour

Agilent Lab Innovation Day for Academia

Is Your LC Method Ready for Today’s Biopharma Challenges? Parsippany, NJ

Webinars

The Journey of Safer Science through Safer Labs

Strategies to Mitigate Sporadic PFAS Contamination in Analytical Workflows

Tracking irrigation-related contaminants in water and soils (QTOF-MS)