KNAUER RF-20Axs Fluorescence Detector

The KNAUER RF-20Axs Fluorescence Detector is the high-performance version of the RF-20A fluorescence detector series, designed for laboratories requiring maximum sensitivity, exceptional reproducibility, and advanced validation capabilities. Covering a wavelength range from 200 to 750 nm, the detector supports both routine and demanding high-performance HPLC applications.

With a signal-to-noise ratio of at least 2000 for the water Raman band, the RF-20Axs is ideally suited for trace-level analysis. The temperature-controlled flow cell with integrated cooling function ensures outstanding stability, reproducibility, and sensitivity, even during long analytical sequences. The detector’s fast 10 ms response time supports ultra-fast chromatography without compromising chromatographic resolution.

The RF-20Axs enables highly sensitive multi-component analysis through programmable wavelength switching during a chromatographic run. An integrated low-pressure mercury lamp allows convenient verification of wavelength accuracy, supporting validation and compliance requirements. Both the xenon lamp and flow cell are easily accessible from the front panel, and lamp replacement requires no optical adjustment. Combined with a lamp lifetime of approximately 2,000 hours, this minimizes downtime and operating costs.

Key Features

• High-sensitivity fluorescence detector for HPLC applications
• Wavelength range from 200 to 750 nm
• Signal-to-noise ratio ≥2000 (water Raman band)
• Designed for trace-level analysis
• Temperature-controlled flow cell with cooling function
• Fast 10 ms response time
• Programmable wavelength switching during analysis
• Simultaneous multi-component analysis at optimal wavelengths
• Integrated low-pressure mercury lamp for wavelength verification
• Long-life xenon lamp with approximately 2,000 operating hours
• Front-accessible lamp and flow cell
• No optical realignment required after lamp replacement
• Advanced validation and support functions
• Stand-alone operation via keypad and display
• Compatible with ClarityChrom®, OpenLAB®, and PurityChrom® software
• Easy integration into virtually any LC system

Technical Specifications

Detection System

• Detection channels: 2
• Number of signals: 2
• Light source:
  • Xenon lamp
  • Low-pressure mercury lamp for wavelength accuracy verification
• Wavelength range: 200–750 nm
• Spectral bandwidth: 20 nm
• Wavelength accuracy: ±2 nm
• Wavelength precision: ±0.2 nm

Sensitivity

• Signal-to-noise ratio: ≥2000 (water Raman band)
• Sensitivity settings:
  • HIGH (×1)
  • MED (×32)
  • LOW (×1024)

Flow Cell

• Standard flow cell included
• Temperature-controlled flow cell
• Integrated cooling function
• Flow cell volume: 12 µL
• Wetted materials:
  • SUS316L stainless steel
  • PTFE
  • Quartz

Signal Processing

• Response time: 10 ms
• Gain settings: ×1, ×4, ×16

Functions

• Autozero function
• Baseline shift function
• Time-programmed wavelength switching
• Wavelength accuracy verification using integrated mercury lamp

Communication

• RS-232 interface
• Analog output
• Software control
• Stand-alone operation via keypad and display

Environmental Conditions

• Operating temperature: 4–35 °C
• Relative humidity: 20–85% (non-condensing)

Power and Dimensions

• Power supply: AC 220–240 V
• Frequency: 50/60 Hz
• Power consumption: 400 VA
• Dimensions (W × H × D): 260 × 210 × 420 mm
• Weight: 18 kg

Applications

• Trace-level pharmaceutical analysis
• Impurity profiling
• Environmental monitoring
• Food safety testing
• Amino acid analysis
• Vitamin analysis
• PAH analysis
• Biomolecule detection
• Fluorescent labeling applications
• Research and quality control laboratories