<p><strong>Join us for a fascinating conversation with Dr. Subhoja Chakraborty from the University of Central Florida, whose cutting-edge research targets the global challenge of malaria drug resistance. Dr. Chakraborty shares her journey from structure-based drug design during her PhD to her recent collaborative work repurposing human kinase inhibitors as rapid, selective antimalarials against drug-resistant Plasmodium falciparum strains.</strong></p><h4><strong>In this episode, you'll learn:</strong></h4><ul><li>How structure-guided approaches reveal potential drug targets, including cysteine proteases like falcipain-2, and kinases implicated in malaria's complex life cycle.</li><li>The power of repurposing kinase inhibitors originally developed for cancer and other diseases, focusing on compound 12, which blocks hemozoin formation and protein kinase 6—key steps in parasite survival.</li><li>Lab techniques such as affinity, gel filtration, and size exclusion chromatography for recombinant protein purification—and how troubleshooting is crucial for high-yield, stable complexes.</li><li>Advances in mass spectrometry for characterizing protein interactions, validating drug targets, and mapping molecular pathways pivotal to malaria therapeutics.</li><li>Tips and lessons for new researchers facing the challenges of sample preparation, complex protocols, and working as part of a collaborative team.</li></ul><p>Dr. Chakraborty’s insights bridge the gap between basic science and translational drug discovery, offering hope for more effective, targeted malaria treatments in a world where resistance to frontline therapies threatens millions.</p><h4><strong>Referenced Papers:</strong></h4><ul><li>"Plasmodium falciparum protein kinase 6 and hemozoin formation are inhibited by a type II human kinase inhibitor exhibiting antimalarial activity" (Cell Chemical Biology, 2025)</li><li>"New insights of falcipain 2 structure from Plasmodium falciparum 3D7 strain" (BBRC, 2022)</li><li>"Structure-Based Optimization of Protease−Inhibitor Interactions to Enhance Specificity of Human Stefin‑A against Falcipain‑2 from the Plasmodium falciparum 3D7 Strain" (Biochemistry, 2023)</li></ul><p>If you’re a student, scientist, or simply passionate about advances in infectious disease research, this episode offers actionable insights, inspiring advice, and a front-row seat to innovation in combating malaria.</p><h3><strong>Video Transcription</strong></h3><h4><strong>From Structural Biology to Drug Discovery: Chromatography and Mass Spectrometry in Malaria Research</strong></h4><h5><strong>Introduction</strong></h5><p>In this discussion, Subhoja shares insights into her research journey, focusing on malaria drug discovery and the role of modern analytical techniques in biological and biomedical research. The conversation is aimed primarily at undergraduate students and early-career researchers interested in chemistry, biotechnology, and life sciences.</p><h5><strong>Research Focus: Malaria Drug Discovery and Kinase Targets</strong></h5><p>Subhoja is currently involved in malaria drug discovery research at the University of Central Florida, where she has been working since beginning her postdoctoral position two years ago. Her earlier PhD research focused on structure-based drug design in malaria, using a combination of in silico approaches, recombinant protein expression, and X-ray crystallography to determine precise three-dimensional structures of target proteins and their inhibitors.</p><p>This structural perspective provided a foundation for her current work, which centers on drug discovery through compound screening and repurposing. A recent publication from her laboratory explores the use of repurposed mammalian CDK-like kinase inhibitors as potential antimalarial agents. While such kinase inhibitors are well known in cancer and cardiovascular research, their application to malaria targets represents a novel and promising approach.</p><p>Kinases are attractive drug targets because of their central role in cell signaling and growth pathways. By targeting specific kinases involved in different stages of the malaria parasite life cycle—whether asexual growth, gametocytogenesis, or differentiation—it may be possible to significantly inhibit parasite growth and progression.</p><h5><strong>Chromatography in Protein Expression and Purification Workflows</strong></h5><p>Chromatography plays a critical role throughout Subhoja’s research. Her experience spans from basic chromatography techniques during her early training to advanced protein purification workflows using affinity chromatography, gel filtration (size exclusion chromatography), and ion exchange chromatography.</p><p>Recombinant proteins are routinely purified using affinity resins such as nickel or cobalt, followed by further purification to remove contaminants. Fast Protein Liquid Chromatography (FPLC) enables compact, stepwise purification workflows suitable for producing proteins for in vitro assays.</p><p>A recurring challenge in this process is the expression of high-molecular-weight proteins, which often accumulate in insoluble fractions or inclusion bodies. Careful selection of expression vectors, host systems, and induction conditions is therefore essential to maximize yield and solubility.</p><h5><strong>Integrating Size Exclusion Chromatography into the Workflow</strong></h5><p>Size exclusion chromatography (SEC) is typically used after affinity purification to further improve protein purity. Following elution with imidazole or glutathione, desalting is required to prevent buffer components from interfering with downstream assays. This is often achieved using desalting columns such as G-25 or PD-10.</p><p>To remove lower-molecular-weight contaminants, finer SEC media—such as Superdex or Sephacryl columns—are employed. Concentration steps using molecular-weight cut-off filters are then applied to obtain the desired protein concentration for functional studies.</p><p>These iterative purification and troubleshooting steps are a routine part of protein biochemistry and reflect the importance of methodical optimization and careful validation.</p><h5><strong>The Role of Mass Spectrometry in Biomolecular Characterization</strong></h5><p>Mass spectrometry is a key analytical tool in Subhoja’s research pipeline, particularly for target identification and validation. In both malaria and cancer research, MS is used alongside chromatography, immunoprecipitation, and pull-down assays to study protein interactions and molecular recognition events.</p><p>Affinity-based mass spectrometry enables the identification of binding partners and interactors, whether studying proteins, long non-coding RNAs, or protein complexes. Techniques such as native MS, tandem affinity purification, and proximity labeling (e.g., TurboID-based workflows) provide deeper insights into molecular networks and signaling pathways.</p><p>These approaches are essential for understanding how candidate drug targets function within complex biological systems.</p><h5><strong>Sample Preparation Challenges in Biological Research</strong></h5><p>Sample preparation remains one of the most challenging aspects of biological experimentation. Whole-cell lysates contain proteases, nucleases, and other components that can rapidly degrade target molecules. Preventing degradation requires minimizing preparation time, maintaining low temperatures, and using appropriate inhibitor cocktails.</p><p>Reproducibility is also critical: biological experiments require multiple replicates, and variability is often unavoidable. Careful handling, protocol optimization, and continuous troubleshooting are essential to ensure reliable results, whether preparing samples for chromatography, mass spectrometry, sequencing, or immunoprecipitation.</p><h5><strong>Advances in Chromatography and MS for Malaria Research</strong></h5><p>Modern advances in chromatography and mass spectrometry significantly enhance malaria research by enabling precise target identification, quantification, and functional characterization. Drug discovery workflows begin with construct design and recombinant expression, followed by purification and interaction studies.</p><p>In malaria research, stage-specific parasite biology requires careful extraction and analysis of proteins from different life cycle stages. Tandem affinity MS, immunoprecipitation, and in vivo validation studies help confirm target relevance and therapeutic potential.</p><p>Recent work on repurposed kinase inhibitors has shown promising results, including inhibition of hemozoin formation—a key detoxification pathway for the malaria parasite—highlighting the potential of such compounds for further development.</p><h5><strong>Malaria, Drug Resistance, and Global Impact</strong></h5><p>Malaria remains a major global health burden, with over 600,000 deaths annually and widespread drug resistance complicating treatment. Artemisinin resistance, particularly associated with mutations in the K13 propeller domain, has driven the search for new targets and therapies.</p><p>During her PhD, Subhoja focused on falcipain-2, a cysteine protease involved in hemoglobin degradation within the parasite. This pathway is critical for parasite survival and has emerged as a promising drug target. Her work revealed that falcipain-2 operates within a larger protein complex responsible for hemoglobin processing and amino acid release.</p><p>Understanding these molecular mechanisms is essential for developing robust, stage-specific antimalarial strategies.</p><h5><strong>Advice for Early-Career Researchers</strong></h5><p>For researchers just starting out, Subhoja emphasizes the importance of hands-on experience. Working directly with cells, lysates, and analytical tools is essential for truly understanding experimental systems. Failure is an inherent part of research, and perseverance is key.</p><p>Motivation, teamwork, careful sample handling, and thorough preparation all contribute to long-term success. Reading extensively, critically evaluating one’s own work, and appreciating incremental progress are vital aspects of a sustainable research career.</p><p>Every successful experiment—no matter how small—represents meaningful progress and contributes to the broader goals of science and medicine.</p><h5><strong>Closing Remarks</strong></h5><p>The discussion highlights how interdisciplinary approaches combining chromatography, mass spectrometry, structural biology, and biochemistry are driving advances in malaria drug discovery. Through careful experimental design, analytical rigor, and collaborative teamwork, researchers can address complex biological challenges and contribute to global health solutions.</p><blockquote><p><strong>This text has been automatically transcribed from a video presentation using AI technology. It may contain inaccuracies and is not guaranteed to be 100% correct.</strong></p></blockquote><blockquote><h4><strong>Concentrating on Chromatography Podcast</strong></h4><p>Dive into the frontiers of chromatography, mass spectrometry, and sample preparation with host David Oliva. Each episode features candid conversations with leading researchers, industry innovators, and passionate scientists who are shaping the future of analytical chemistry. From decoding PFAS detection challenges to exploring the latest in AI-assisted liquid chromatography, this show uncovers practical workflows, sustainability breakthroughs, and the real-world impact of separation science. Whether you’re a chromatographer, lab professional, or researcher you'll discover inspiring content!</p><p>You can find <strong>Concentrating on Chromatography Podcast </strong>in podcast apps:</p><ul><li><a target="_blank" rel="noopener noreferrer" href="https://open.spotify.com/show/1wZg67rCjoGju4yhqxI3tL"><strong>Spotify</strong></a></li><li><a target="_blank" rel="noopener noreferrer" href="https://podcasts.apple.com/tz/podcast/concentrating-on-chromatography/id1788429185"><strong>Apple podcast</strong></a></li><li><a target="_blank" rel="noopener noreferrer" href="https://podscan.fm/podcasts/concentrating-on-chromatography"><strong>podscan</strong></a></li></ul><p>and on <a target="_blank" rel="noopener noreferrer" href="https://www.youtube.com/@ChromatographyTalk/videos"><strong>YouTube channel</strong></a></p></blockquote>

Affinity, Gel Filtration, Size Exclusion Chromatography: Repurposing Kinase Inhibitors for Malaria

<p><strong>In the week of February 9, the following webinars await you in the field of liquid phase and especially HPLC and LC/MS.</strong></p><blockquote><h4><a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/webinars"><strong>👉 DETAILS AND REGISTRATION IN THE WEBINARS SECTION</strong></a></h4><p><strong>👉 Search and filter in the largest global database</strong> with over <strong>5 000 GC, LC, MS and spectroscopy webinars</strong>.</p></blockquote><h5><strong>1. SelectScience: High-Throughput Stability Screening Strategies for Antibody Formulation</strong></h5><ul><li>Tu, 10.2.2026 17:00 CET</li><li><a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/webinar/5807">Registration</a></li></ul><p>Learn how high-throughput DLS/SLS and rapid-screening DSC accelerate stability assessment of high-concentration antibody formulations, enabling faster, data-driven biologics development.</p><h5><strong>2. Phenomenex: Big Scale, Big Wins – Prep Chromatography Explained</strong></h5><ul><li>We, 11.2.2026 10:00 CET</li><li><a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/webinar/5793">Registration</a></li></ul><p>Learn how to scale analytical methods to preparative HPLC, optimize loading capacity, and understand the limits of preparative columns for pharmaceutical applications.</p><h5><strong>3. ALS Czech Republic: PFAS in Food &amp; Food Contact Material</strong></h5><ul><li>We, 11.2.2026 15:00 CET</li><li><a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/webinar/5816">Registration</a></li></ul><p>Get a practical overview of PFAS in food and food contact materials, including EU and US regulations, key analytical challenges, and reliable PFAS testing strategies.</p><h5><strong>4. New Food Magazine: The Science of Next-Gen Ingredients – Fermentation, Formulation and Function</strong></h5><ul><li>We, 11.2.2026 16:00 CET</li><li><a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/webinar/5720">Registration</a></li></ul><p>Explore how fermentation-driven innovation is transforming functional food ingredients and how advanced analytics optimize flavour, functionality, and stability.</p><h5><strong>5. SCIEX: Accurate Mass Fundamentals – Interpreting and Applying Your Data</strong></h5><ul><li>We, 11.2.2026 19:00 CET</li><li><a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/webinar/5772">Registration</a></li></ul><p>Session 3 focuses on interpreting and applying accurate mass data to confirm compound identity, move beyond basic quantitation, and troubleshoot results for confident reporting.</p><h5><strong>6. Agilent Technologies: Process Analytical Techniques – Online HPLC for Real-Time Monitoring and Control of Bioprocesses</strong></h5><ul><li>Th, 12.2.2026 06:30 CET</li><li><a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/webinar/5834">Registration</a></li></ul><p>Discover how PAT HPLC enables near-real-time monitoring of critical quality attributes in bioprocessing, with a case study on charge-variant control by cation-exchange chromatography.</p><h5><strong>7. Thermo Fisher Scientific: Introducing the Orbitrap Excedion Pro Hybrid MS</strong></h5><ul><li>Th, 12.2.2026 11:00 CET</li><li><a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/webinar/5889">Registration</a></li></ul><p>Discover the new Orbitrap Exploris Pro with expanded ETD/EThcD capabilities for confident biopharmaceutical characterization, including intact proteins, PTMs, ADCs, and large biomolecules.</p><h5><strong>8. Waters Corporation: Planning Your NuGenesis Upgrade – Insights and Best Practices for a Smooth Transition</strong></h5><ul><li>Th, 12.2.2026 19:00 CET</li><li><a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/webinar/5833">Registration</a></li></ul><p>Learn how to plan a successful NuGenesis upgrade, transition to Windows 11, and unlock new capabilities such as dashboards for real-time operational insights.</p><h5><strong>9. Thermo Fisher Scientific: Comprehensive Analytical Strategies for Peptide Therapeutics and GLP-1 Agonists with Agilent</strong></h5><ul><li>Th, 12.2.2026 19:00 CET</li><li><a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/webinar/5814">Registration</a></li></ul><p>Explore analytical workflows for peptide therapeutics, including method development, peptide mapping, GLP-1 QC, and LC-MS strategies across Agilent platforms.</p><h5><strong>10. Agilent Technologies: Moving from R&amp;D and Limited QC on a UV-Vis-NIR Instrument to Large-Scale QC Using an Autosampler</strong></h5><ul><li>Th, 12.2.2026 20:00 CET</li><li><a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/webinar/2104">Registration</a></li></ul><p>This webinar covers practical aspects of adding and using an autosampler to maximize UV-Vis-NIR QC workflows at scale.</p><h5><strong>11. Agilent Technologies: LC Method Transfer – Hidden Challenges &amp; Absolute Solution</strong></h5><ul><li>Fr, 13.2.2026 06:30 CET</li><li><a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/webinar/5787">Registration</a></li></ul><p>Discover why LC method transfer between legacy and modern systems is challenging, which hidden parameters affect retention and peak shape, and how to achieve robust, reliable results.</p>

Webinars LabRulezLCMS Week 07/2026

<p><strong>Charge detection mass spectrometry (CDMS) allows direct mass determination of individual ions and is increasingly used to study heterogeneous biological samples. This work demonstrates the use of Orbitrap-based CDMS/Direct Mass Technology (DMT) for quantitative analysis of transthyretin (TTR) tetramer samples below 100 kDa, extending CDMS beyond its traditional qualitative role.</strong></p><p>DMT resolved wild-type and C-terminally modified TTR homotetramers, as well as hybrid tetramers, enabling kinetic analysis of subunit exchange and stability. The approach also quantified thyroxine binding to TTR variants, revealing differences in binding affinity linked to C-terminal modification. By separating overlapping m/z signals through charge measurement, CDMS enables accurate mass and abundance determination, highlighting its potential for quantitative analysis of complex protein systems.</p><blockquote><h4><strong>The original article</strong></h4><h4><a target="_blank" rel="noopener noreferrer" href="https://pubs.acs.org/doi/10.1021/acs.analchem.5c05771"><strong>Dissecting Heterogeneous Populations of Protein-Complex Samples Using Direct Mass Technology</strong></a></h4><p>Robert L. Rider, Jared Hampton, Zhenyu Xi, Carter Lantz, Sangho D. Yun, Weijing Liu, Rosa Viner, Arthur Laganowsky, and David H. Russell*</p><p>Anal. Chem. 2025, 97, 49, 27057–27063</p><p><a target="_blank" rel="noopener noreferrer" href="https://doi.org/10.1021/acs.analchem.5c05771">https://doi.org/10.1021/acs.analchem.5c05771</a></p><h6>licensed under <a target="_blank" rel="noopener noreferrer" href="https://creativecommons.org/licenses/by/4.0/"><strong>CC-BY 4.0</strong></a></h6></blockquote><p><strong>Selected sections from the article follow. Formats and hyperlinks were adapted from the original.</strong></p><p>Transthyretin (TTR) is a homotetrameric transport protein that circulates in plasma and cerebrospinal fluid, delivering thyroxine (T<sub>4</sub>) and retinol-binding protein–vitamin A complexes. <a href="javascript:void(0);">(1)</a> The stability of the TTR tetramer is central to its physiological role because dissociation into monomers can initiate misfolding and amyloid formation, leading to systemic or hereditary transthyretin amyloidosis. <a href="javascript:void(0);">(2)</a> Subunit exchange between wild-type (WT) and variant TTR proteoforms, including disease-associated mutations such as V30M and V122I, have been implicated in amyloid progression by generating hybrid tetramers (tetramers containing WT and mutant subunits) with altered stabilities. <a href="javascript:void(0);">(3,4)</a> Ligand binding, particularly at the two thyroxine-binding sites located at the dimer–dimer interface, can kinetically stabilize the tetramer and modulate amyloidogenicity. <a href="javascript:void(0);">(5,6)</a> Accurate measurement of both subunit exchange kinetics and ligand binding stoichiometry is therefore critical for elucidating the TTR dynamics and stability.</p><p>Affinity or sequence tags are widely employed in protein expression, purification, or detection. <a href="javascript:void(0);">(7,8)</a> While these tags are often assumed to be inert, their influence on protein structure and dynamics are rarely examined in detail. <a href="javascript:void(0);">(9,10)</a> Protein tags at the N- and C-terminus of TTR have been shown to have differing gas phase stabilities from those of WT-TTR. <a href="javascript:void(0);">(11)</a> The C-terminus is of particular interest because this region participates in the dimer–dimer interface that governs tetramer stability. <a href="javascript:void(0);">(11,12)</a> Characterizing how the C-terminal tag (CT) (-ASGENLFYQ) affects TTR’s solution stability and ligand binding is not only relevant for understanding TTR dynamics but also provides an example of how expression tags can alter protein dynamics. <a href="javascript:void(0);">(13)</a></p><p>Native mass spectrometry (nMS) has been invaluable for characterizing protein complexes, <a href="javascript:void(0);">(14)</a> yet conventional nMS often struggles with heterogeneous samples where overlapping charge state distributions and adduct heterogeneity limit resolution and quantitation. <a href="javascript:void(0);">(15)</a> Charge detection mass spectrometry (CDMS) addresses these limitations by independently measuring the charge and <i>m</i>/<i>z</i> of individual ions, enabling the direct determination of molecular mass and abundance. Development of CDMS has largely focused on achieving higher mass resolution <a href="javascript:void(0);">(16)</a> and extending measurements toward assemblies into the GDa range. <a href="javascript:void(0);">(17,18)</a> However, there is untapped utility for CDMS experiments other than mass and proteoform determination. Jordan et al. showed the capability to assess conformational heterogeneity of mAb aggregates using the added charge dimension that CDMS provides. <a href="javascript:void(0);">(19)</a> The current work demonstrates that CDMS can separate proteoforms with overlapping <i>m</i>/<i>z</i> values based on charge and quantify their relative abundances in complex mixtures. While nMS is already a highly quantitative technique, our results establish CDMS as an equally powerful tool for quantitative analysis in heterogeneous systems. <a href="javascript:void(0);">(20,21)</a></p><p>Here, we apply Orbitrap-based CDMS/Direct Mass Technology (DMT) using the selective temporal overview of resonant ions (STORI) approach <a href="javascript:void(0);">(22,23)</a> to quantitatively compare WT-TTR and CT-TTR, focusing on two biologically relevant properties: tetramer stability assessed via subunit exchange kinetics and ligand-binding affinity. Conventional nMS cannot fully resolve the overlapping charge states of WT- and CT-TTR or their ligand-bound states. However, DMT can resolve these species by directly measuring the mass (<i>m</i>/<i>z</i> × charge), enabling quantitation of all proteoforms. The data reveal that the CT tag alters tetramer stability and thyroxine-binding affinity, demonstrating the structural sensitivity of TTR to modifications at its C-terminus. More broadly, this work demonstrates the impact that protein tags can have on native protein interactions and highlights the broader utility of quantitative CDMS for probing heterogeneous biological samples.</p><h4><strong>Experimental Section</strong></h4><h5><strong>Data Collection</strong></h5><p>All experiments were conducted on a <a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/products/560"><strong>Thermo Scientific Q-Exactive UHMR</strong></a> with added DMT capabilities (Bremen, Germany). Before DMT, mass spectra were collected at various resolving powers (6–200k) for each sample, with the instrument parameters reported in the Supporting Information (<a target="_blank" rel="noopener noreferrer" href="https://pubs.acs.org/doi/suppl/10.1021/acs.analchem.5c05771/suppl_file/ac5c05771_si_001.pdf">Table S1</a>). For DMT experiments, the trapping gas pressure in the HCD cell was reduced to 0.3–0.8 to attenuate the signal intensity to a single ion level, as well as decreasing the UHV pressure to assist ion retention through the entire transient of Orbitrap analysis. We observed no significant difference between mass spectra collected at various HCD cell pressures (Supporting Information, <a target="_blank" rel="noopener noreferrer" href="https://pubs.acs.org/doi/suppl/10.1021/acs.analchem.5c05771/suppl_file/ac5c05771_si_001.pdf">Figure S1</a>). For DMT collection, automated ion control (AIC) and 100% target density were used with 200k resolving power.</p><h4><strong>Results and Discussion</strong></h4><p>CDMS has emerged as a powerful tool for analyzing heterogeneous biomolecular samples, enabling accurate, direct mass determination by simultaneously measuring the charge and <i>m</i>/<i>z</i> for individual ions. While CDMS has been widely applied for qualitative analyses, its potential for quantitative and less than MDa applications remains underexplored. <a target="_blank" rel="noopener noreferrer" href="https://pubs.acs.org/doi/10.1021/acs.analchem.5c05771#fig1">Figure 1</a>A shows the theoretical mass spectrum of WT-TTR (14+ to 16+) and CT-TTR (15+ to 17+) homotetramers, where the two proteoforms become increasingly resolved as the charge states increase (&lt;3900 <i>m</i>/<i>z</i>). <a target="_blank" rel="noopener noreferrer" href="https://pubs.acs.org/doi/10.1021/acs.analchem.5c05771#fig1">Figure 1</a>B zooms in on the 14+ (WT) and 15+ (CT) charge states, highlighting their strong overlap, which prevents accurate deconvolution of the proteoforms by conventional methods. <a target="_blank" rel="noopener noreferrer" href="https://pubs.acs.org/doi/10.1021/acs.analchem.5c05771#fig1">Figure 1</a>C displays theoretical distributions for 0, 1, and 2 T<sub>4</sub> bound states of both WT- and CT-TTR tetramers, where &lt;3900 <i>m</i>/<i>z</i> has observable separation between all proteoforms. However, for the 14+ and 15+ charge states of WT- and CT-TTR, respectively, the 0 T<sub>4</sub>- and 1 T<sub>4</sub>-bound populations overlap (<a target="_blank" rel="noopener noreferrer" href="https://pubs.acs.org/doi/10.1021/acs.analchem.5c05771#fig1">Figure 1</a>D). Furthermore, commonly adducted species (Na<sup>+</sup>, K<sup>+</sup>, and Zn<sup>2+</sup>) observed in experimental spectra broaden these distributions, making it increasingly difficult to distinguish and deconvolute the proteoforms (see <a target="_blank" rel="noopener noreferrer" href="https://pubs.acs.org/doi/suppl/10.1021/acs.analchem.5c05771/suppl_file/ac5c05771_si_001.pdf">Figure S2</a>). Here, we employ Orbitrap-based CDMS/DMT to overcome this <i>m</i>/<i>z</i> overlap to resolve and quantify hybrid tetramer formation and T<sub>4</sub> binding.</p><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Anal_Chem_2025_97_49_27057_27063_Figure_1_A_Theoretical_mass_spectra_of_WT_TTR_and_CT_TTR_homotetramers_47232849d9.jpg" alt="Anal. Chem. 2025, 97, 49, 27057–27063: Figure 1. (A) Theoretical mass spectra of WT-TTR and CT-TTR homotetramers. (B) Zoomed-in view of the 14+ WT-TTR and 15+ CT-TTR distributions to show the large overlap of the proteoforms. (C) Theoretical mass spectra of WT-TTR and CT-TTR homotetramers with thyroxine (T4) binding. (D) Zoomed-in view of the 14+ WT-TTR and 15+ CT-TTR distributions where the 0 and 1 T4 have overlapping distributions. These theoretical distributions do not contain any commonly adducted species (Na+, K+, Zn2+), which broaden the distributions and create more overlap even for the proteoforms in the range 3300–3900 m/z (see Figure S2)." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Anal_Chem_2025_97_49_27057_27063_Figure_1_A_Theoretical_mass_spectra_of_WT_TTR_and_CT_TTR_homotetramers_47232849d9.jpg 187w," sizes="100vw" width="1417" height="1182"></p><h5><strong>Thyroxine Binding to TTR Homotetramers</strong></h5><p>Thyroxine is the less active form of a thyroid hormone important for metabolism that binds to TTR for transport throughout plasma and cerebral spinal fluid. <a href="javascript:void(0);">(31)</a> Turnover of T<sub>4</sub> into T<sub>3</sub> (active) occurs after dissociation, where it can then modulate gene expression via binding to nuclear receptors. <a href="javascript:void(0);">(32)</a> The TTR tetramer gains substantial stability as it binds up to 2 T<sub>4</sub> at the tetramer interface, decreasing tetramer dissociation and SUE. <a href="javascript:void(0);">(33)</a> This has been shown previously to decrease the amyloidogenic rate of TTR, which led to tafamidis (similar in structure to T<sub>4</sub>) to be designed and is currently the only drug that is approved for clinical treatment of TTR amyloidosis. <a href="javascript:void(0);">(34)</a> Measuring the difference in binding affinity between TTR proteoforms may provide insight into regions of TTR or TTR intermolecular/intramolecular interactions that are important for binding of small molecules such as tafamidis and T<sub>4</sub>.</p><p>DMT was further applied to quantify T<sub>4</sub> binding to WT- and CT-TTR homotetramers, highlighting the technique’s utility in probing protein–ligand interactions. <a target="_blank" rel="noopener noreferrer" href="https://pubs.acs.org/doi/10.1021/acs.analchem.5c05771#fig3">Figure 3</a>A shows a representative mass spectrum of TTR homotetramers in the presence of T<sub>4</sub>. While ligand-bound species can be observed, homotetramers cannot be confidently resolved across the entire 3000–5000 <i>m</i>/<i>z</i> range. By leveraging the charge-resolving capabilities of DMT, we achieve separation of the homotetramers (<a target="_blank" rel="noopener noreferrer" href="https://pubs.acs.org/doi/10.1021/acs.analchem.5c05771#fig3">Figure 3</a>B,C) and the 0, 1, and 2 ligand-bound states. The 2D plot clearly shows the gain in resolution achieved when charge is simultaneously measured with <i>m</i>/<i>z</i> by separating the 14+ and 15+ charge states for WT and CT, respectively, in the range 3900–4200 <i>m</i>/<i>z</i>. Additionally, using ion mobility does not provide the same kind of 2D resolution due to the similarity in arrival times for the homotetramer proteoforms, as evidenced by <a target="_blank" rel="noopener noreferrer" href="https://pubs.acs.org/doi/suppl/10.1021/acs.analchem.5c05771/suppl_file/ac5c05771_si_001.pdf">Figures S4 and S5</a> (see the Supporting Information). These data provide evidence that when ion mobility is able to separate out species that overlap in <i>m</i>/<i>z</i> yet differ in mobility, CDMS could be an alternative or used complementarily since the species likely also differ in charge.</p><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Anal_Chem_2025_97_49_27057_27063_Figure_3_A_Mass_spectra_of_WT_TTR_and_CT_TTR_homotetramers_with_thyroxine_binding_61b1e98a94.jpg" alt="Anal. Chem. 2025, 97, 49, 27057–27063: Figure 3. (A) Mass spectra of WT-TTR and CT-TTR homotetramers with thyroxine binding. Ligand binding can be resolved, but homotetramers cannot be confidently identified. Charge states labeled are for WT; CT is always one charge state higher at the same m/z range. (B) DMT heatmap resolving WT-TTR and CT-TTR homotetramers with the additional measurement of charge. (C) Deconvoluted mass spectrum from DMT analysis. Both homotetramers and their ligand binding are resolved, and fit for analysis is shown in red. (D) Mole fraction of each homotetramer and their thyroxine binding species. Error bars represent the standard deviation of replicates (n = 3). The significance for each ligand state is in respect to the left adjacent state. *p &lt; 0.05." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Anal_Chem_2025_97_49_27057_27063_Figure_3_A_Mass_spectra_of_WT_TTR_and_CT_TTR_homotetramers_with_thyroxine_binding_61b1e98a94.jpg 181w," sizes="100vw" width="1266" height="1094"></p><h4><strong>Conclusion</strong></h4><p>This work demonstrates that quantitatively, Orbitrap-based CDMS/DMT can determine differences in protein-complex stability and ligand binding. By directly comparing WT- with CT-TTR, we show that the CT tag accelerates tetramer dissociation while enhancing T<sub>4</sub> binding affinity. These results indicate that the C-terminus, located at the dimer–dimer interface, plays a more significant role in TTR stability and ligand recognition than previously appreciated. <a href="javascript:void(0);">(12)</a> Even the addition of a short peptide sequence perturbs noncovalent interactions at the interface, likely altering solvent accessibility, and the dynamics of the thyroxine binding channel. <a href="javascript:void(0);">(37,54)</a> Such findings underscore the importance of evaluating how seemingly minor sequence modifications, such as affinity tags, can influence fundamental biophysical properties.</p><p>Beyond TTR, this work positions quantitative CDMS as a broadly applicable strategy for analyzing heterogeneous protein systems, where charge domain separation overcomes the resolution limits of <i>m</i>/<i>z</i> domain measurements in traditional nMS experiments. While much of the field has emphasized extending CDMS to ultrahigh masses and maximal resolution, <a href="javascript:void(0);">(16,18)</a> our results highlight the underexplored potential of applying the method in the 1000–10,000 <i>m</i>/<i>z</i> range common to nMS experiments. This capability is directly relevant to heterogeneous membrane protein complexes, which often display broad distributions of oligomeric states, PTMs, lipid, and ligand adducts that complicate spectra. <a href="javascript:void(0);">(48,55)</a> Similarly, mAbs can oligomerize and exhibit mass heterogeneity from variable glycosylation, affecting pharmacokinetics, effector function, and stability. <a href="javascript:void(0);">(19)</a> CDMS may resolve and quantify such proteoforms in a single experiment without the need for charge reduction or chemical derivatization. <a href="javascript:void(0);">(48,56,57)</a></p>

Dissecting Heterogeneous Populations of Protein-Complex Samples Using Direct Mass Technology

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DataApex CE - Capillary Electrophoresis Extension for Clarity

CE module provides specific terminology, evaluation by time corrected area and peak identification by peak start or end (additionally to peak apex).

<h3><strong>Extension for Capillary Electrophoresis</strong></h3>
<p>The CE Extension <strong>activates the CE mode in Clarity</strong> environment and can be activated <strong>on any <a href="https://lcms.labrulez.com/products/999">Clarity</a> Instrument</strong>. CE Extension is compatible also with <strong><a href="https://lcms.labrulez.com/products/1000">Clarity Offline</a> software</strong>. CE module <strong>provides specific terminology</strong>, <strong>evaluation by time corrected area</strong> and peak identification by peak start or end (additionally to peak apex).</p>
<h4><strong>Main changes in Clarity environment</strong></h4>
<ul>
<li>CE specific terminology</li>
<li>Evaluation by time corrected area</li>
<li>Peak identification by peak start or end (additionally to peak apex).</li>
<li>Additional calculations</li>
</ul>
<h4><strong>CE specific terminology</strong></h4>
<p>The terms used in chromatography mode were substituted by those used in CE:</p>
<ul>
<li>Default mode =&gt; CE mode</li>
<li>Chromatogram =&gt; Electropherogram</li>
<li>Retention time (RT) =&gt; Migration Time (MT)</li>
<li>Column =&gt; Capillary</li>
<li>Mobile Phase =&gt; Electrolyte</li>
<li>Flow Rate =&gt; Power supply</li>
<li>Pressure =&gt; Injection Mode</li>
</ul>
<h4><strong>Separation parameters</strong></h4>
<p>Method Setup allows a user to adjust the Capillary Setting:</p>
<ul>
<li>Total Capillary Length</li>
<li>Length of the capillary.</li>
<li>Effective Capillary Length</li>
<li>Effective capillary length from injection end to the detector position</li>
<li>Separation Voltage</li>
<li>Separation voltage. Allows you to enter also negative values</li>
<li>Reference Peak Mobility</li>
<li>Effective mobility of the EOF reference peak.</li>
</ul>
<h4><strong>Calculations</strong></h4>
<p>The Result Table contains additional columns containing calculations:</p>
<ul>
<li>Apparent Mobility</li>
<li>Effective Mobility</li>
</ul>


Wiley KnowItAll Analytical Edition Software

KnowItAll by Wiley offers a comprehensive solution for identifying, analyzing, and managing analytical data, enhancing spectral analysis speed and overall lab efficiency.

<p class="cke-user_style-important"><strong>Solutions for IR, MS, NMR, Raman, &amp; UV-Vis</strong></p><blockquote><p><strong>More information on the Wiley Science Solutions website: </strong><a target="_blank" rel="noopener noreferrer" href="https://sciencesolutions.wiley.com/knowitall-analytical-edition-software/"><strong>KnowItAll Analytical Edition Software</strong></a></p></blockquote><h5><strong data-start="4" data-end="56">Accelerate Spectral Analysis &amp; Boost Efficiency!</strong></h5><p data-start="60" data-end="206"><strong data-start="60" data-end="73">KnowItAll</strong> by <strong data-start="77" data-end="86">Wiley</strong> is an <strong data-start="93" data-end="107" data-is-only-node="">all-in-one</strong> solution for <strong data-start="121" data-end="161">identifying, analyzing, and managing</strong> spectral data, streamlining lab workflows.</p><ul data-start="208" data-end="581" data-is-only-node="" data-is-last-node=""><li data-start="208" data-end="328"><strong data-start="210" data-end="237">User-friendly interface</strong> with tools for <strong data-start="253" data-end="326">processing, searching, mixture analysis, deconvolution, and reporting</strong></li><li data-start="329" data-end="406"><strong data-start="331" data-end="365">Patented spectral intelligence</strong> for automated, expert-driven workflows</li><li data-start="407" data-end="481"><strong data-start="409" data-end="458">Supports multiple techniques &amp; 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Molecular Structures</strong></li></ul><h3 data-start="520" data-end="542"><strong data-start="524" data-end="540">Key Features</strong></h3><p data-start="543" data-end="699">Eliminate the need for multiple software packages—KnowItAll <strong data-start="603" data-end="657">combines powerful tools in one intuitive interface</strong> for seamless transitions between tasks.</p><ul data-start="701" data-end="998"><li data-start="701" data-end="750"><strong data-start="703" data-end="748">Comprehensive Spectrum &amp; Mixture Analysis</strong></li><li data-start="751" data-end="799"><strong data-start="753" data-end="797">Database Management &amp; Advanced Searching</strong></li><li data-start="800" data-end="863"><strong data-start="802" data-end="848">Structure Drawing &amp; Professional Reporting</strong> (ChemWindow)</li><li data-start="864" data-end="907"><strong data-start="866" data-end="905">Spectrum Processing &amp; Deconvolution</strong></li><li data-start="908" data-end="956"><strong data-start="910" data-end="954">Extensive Spectral Library Subscriptions</strong></li><li data-start="957" data-end="998"><strong data-start="959" data-end="996">Quantitative Analysis Module</strong></li><li data-start="1030" data-end="1072"><strong data-start="1032" data-end="1070">IR/Raman functional group analysis</strong></li><li data-start="1073" data-end="1128"><strong data-start="1075" data-end="1107">MS reverse &amp; adaptive search</strong> <em data-start="1108" data-end="1126"><i>(patente)</i></em></li><li data-start="1129" data-end="1174"><strong data-start="1131" data-end="1172">Automated MS deconvolution &amp; analysis</strong></li><li data-start="1175" data-end="1212"><strong data-start="1177" data-end="1210">NMR chemical shift prediction</strong></li></ul><h4><strong>What’s Included?</strong></h4><h5><strong data-start="1245" data-end="1261">Data Toolbox</strong></h5><ul data-start="1264" data-end="1540"><li data-start="1264" data-end="1318"><strong data-start="1266" data-end="1280">ID Expert™</strong> – One-click spectral identification</li><li data-start="1319" data-end="1411"><strong data-start="1321" data-end="1334">SearchIt™</strong> – Advanced database search with mixture analysis &amp; multi-technique support</li><li data-start="1412" data-end="1470"><strong data-start="1414" data-end="1425">MineIt™</strong> – Spectral database creation &amp; data mining</li><li data-start="1471" data-end="1540"><strong data-start="1473" data-end="1487">QC Expert™</strong> – Quality control for IR, Raman, and Chromatograms</li></ul><h5><strong data-start="1547" data-end="1578">Spectral Processing Toolbox</strong></h5><ul data-start="1581" data-end="1716"><li data-start="1581" data-end="1652"><strong data-start="1583" data-end="1597">MS Expert™</strong> – GC-MS deconvolution with automated database search</li><li data-start="1653" data-end="1716"><strong data-start="1655" data-end="1669">ProcessIt™</strong> – Spectrum processing for IR, MS, NMR, Raman</li></ul><h5><strong data-start="1723" data-end="1752">Spectral Analysis Toolbox</strong></h5><ul data-start="1755" data-end="1882"><li data-start="1755" data-end="1827"><strong data-start="1757" data-end="1771">AnalyzeIt™</strong> – Functional group analysis for IR, Raman, &amp; polymers</li><li data-start="1828" data-end="1882"><strong data-start="1830" data-end="1848">PredictIt™ NMR</strong> – NMR chemical shift prediction</li></ul><h5><strong data-start="1889" data-end="1907">Basics Toolbox</strong></h5><ul data-start="1910" data-end="2115"><li data-start="1910" data-end="1965"><strong data-start="1912" data-end="1926">ChemWindow</strong> – Full-featured 2D structure drawing</li><li data-start="1966" data-end="2049"><strong data-start="1968" data-end="1981">ReportIt™</strong> – Professional reporting for structures, spectra, &amp; chromatograms</li><li data-start="2050" data-end="2115"><strong data-start="2052" data-end="2065">BrowseIt™</strong> – Web portal with KnowItAll resources &amp; updates</li><li data-start="2050" data-end="2115"><strong data-start="2121" data-end="2153">Optional Quantitation Module - </strong>Enhance <strong data-start="2164" data-end="2200">GC-MS, IR, Raman, UV-Vis, and GC</strong> workflows with integrated quantification.</li></ul><h5><strong data-start="2250" data-end="2292">Access High-Quality Spectral Libraries</strong></h5><ul><li><p data-start="2295" data-end="2423" data-is-last-node="" data-is-only-node="">Subscribe to <strong data-start="2308" data-end="2386">Wiley’s industry-leading IR, MS, NMR, Raman, and UV-Vis spectral databases</strong> for <strong data-start="2391" data-end="2402">optimal</strong> analytical accuracy.</p></li></ul><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Wiley_Wiley_Know_It_All_Analytical_Edition_Software_Know_It_All_Spectral_Libraries_2fdbf59fd0/Wiley-Wiley-KnowItAll-Analytical-Edition-Software-KnowItAll-Spectral-Libraries.jpg" alt="Wiley: Wiley KnowItAll Analytical Edition Software - KnowItAll Spectral Libraries" width="474" height="343"></p><h4><strong>System Recommendations &amp; Compatibility</strong></h4><ul><li><a target="_blank" rel="noopener noreferrer" href="https://sciencesolutions.wiley.com/system-recommendations/">See system recommendations here.</a></li><li><a target="_blank" rel="noopener noreferrer" href="https://sciencesolutions.wiley.com/knowitall-supported-file-formats/">View file formats supported.</a></li></ul><p>*Subscription required to KnowItAll Spectral Libraries.</p>

Modern HPLC separations in theory and practice

This book is a unique combination of theory and practice, but also overviews of stationary phases, sample prep materials, and vendor’s brief presentations.

SIELC Alltesta Gradient Automated HPLC Analyzer

SIELC’s Alltesta™ Analyzer is an affordable HPLC analytical system with for measuring small and trace concentrations of various chemicals.

<p><strong>SIELC’s Alltesta™ Analyzer is an affordable HPLC analytical system that provides both scientific and educational environments with a convenient, simple solution for measuring small and trace concentrations of various chemicals in products such as milk, baby formula, pet food, pills, household formulations, and more.</strong></p><p>No difficult sample preparation is necessary, and no prior experience in chromatography or analytical chemistry is required for the operator. Measurements typically take less than 5 minutes, and the Alltesta™ comes with a set of supplies sufficient for 200 analyses.</p><ul><li><strong>Small foot-print</strong>, <strong>light-weight</strong>, can be used on a small table</li><li>No difficult sample preparation</li><li>Takes only a few <strong>minutes to start</strong> operating</li><li><strong>No chemical experience is required</strong> for instrument user</li><li><strong>User friendly</strong>, self explanatory <strong>cloud-based software</strong></li><li>Produces easy-to-understand <strong>results within 5-10 minutes</strong></li></ul><h4><strong>Alltesta Gradient Automated HPLC Analyzer includes:</strong></h4><ul><li><a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/products/1249"><strong>Alltesta™ Power Tower</strong></a> (provides power and cloud connectivity)</li><li><a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/products/1244"><strong>Alltesta Mini Autosampler</strong></a> (includes <a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/products/1245"><strong>Alltesta High-Pressure Switching Valve</strong></a>)</li><li>Two <a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/products/1247"><strong>Alltesta™ Ultra High Pressure Mini Syringe Pumps</strong></a> for gradient flow</li><li><a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/products/1248"><strong>Alltesta UV-Vis Detector</strong></a></li></ul><h4><strong>Examples of Alltesta™-based HPLC methods</strong></h4><ul><li><a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/labrulez-bucket-strapi-h3hsga3/HPLC_Method_for_Analysis_of_Aspirin_in_Alka_Seltzer_Extra_Strength_Effervescent_Aspirin_on_Primesep_100_Column_d33816ae83.pdf"><strong>HPLC Method for Analysis of Aspirin in Alka-Seltzer Extra Strength Effervescent Aspirin</strong></a></li><li><a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/labrulez-bucket-strapi-h3hsga3/HPLC_Method_for_Analysis_of_Phenylephrine_Hydrochloride_1_in_Equate_Nasal_Spray_on_Primesep_100_Column_02f2983c25.pdf"><strong>HPLC Method for Analysis of Phenylephrine Hydrochloride 1% in Equate – Nasal Spray</strong></a></li><li><a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/labrulez-bucket-strapi-h3hsga3/HPLC_Method_for_Analysis_of_Rockstar_Zero_Carbs_Energy_Drink_with_Caffeine_and_Taurine_on_Primesep_100_Column_c87a85a0b4.pdf"><strong>HPLC Method for Analysis of Rockstar Zero Carbs Energy Drink with Caffeine and Taurine</strong></a></li></ul><p>See this cloud-based example of the <strong>2-Naphthalenesulfonic acid, Allura red, Fast Green FCF, 1,5-Naphthalenedisulfonic acid, Tartrazine</strong> separation method <a target="_blank" rel="noopener noreferrer" href="https://chrom.app/share/#/samples/11972/injection/21307/?t=a992fefcdd3647b19f6bed81e182d3d8"><strong>here</strong></a>.</p><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/SIELC_SIELC_Alltesta_Gradient_Automated_Analyzer_example_of_the_2_Naphthalenesulfonic_acid_Allura_red_Fast_Green_FCF_1_5_Naphthalenedisulfonic_acid_Tartrazine_separation_b96e648d63.jpg" alt="SIELC: SIELC Alltesta™ Gradient Automated Analyzer - example of the 2-Naphthalenesulfonic acid, Allura red, Fast Green FCF, 1,5-Naphthalenedisulfonic acid, Tartrazine separation."></p>

SIELC Alltesta Cromite HPLC Analyzer

The Cromite™ Analyzer represents a new generation of affordable and easy-to-learn HPLC instrumentation for schools, colleges and universities.

<p><strong>With automated single-vial injection, high-pressure flow and UV/Vis detection capabilities, scholars can use this instrument to separate compounds, such as vitamins in juices, food dyes, caffeine and active ingredients in pharmaceutical products. The free cloud-connected software allows you to run tests, analyze results and share data from anywhere and with any device.</strong></p><p>SIELC Technologies provides a vast library of free methods for a variety of compounds, enabling students to characterize and understand the chemical composition of many different products. The Cromite™ Analyzer comes with a column of your choice and all necessary accessories to perform multiple analyses.</p><blockquote><p class="cke-user_style-important"><strong>We offer a special 40% discount for schools. (From 9 000 Eur excl. VAT)</strong></p></blockquote><h4><strong>Key Characteristics</strong></h4><ul><li><strong>Miniature size</strong> (WHL 115 x 190 x 115 mm / 4.5 x 7.5 x 4.5 in)</li><li>Single <strong>auto injection</strong></li><li>Pressure <strong>up to 4000 psi</strong></li><li><strong>Columns with 3 and 2 mm ID</strong></li><li><strong>Real-time data streaming</strong></li><li><strong>Optical signal monitoring</strong> (up to 4 wavelengths simultaneously)</li><li>Continuous <strong>pressure monitoring</strong></li><li>Create, store, and access <strong>methods</strong>, <strong>sample information</strong> and <strong>analysis results in the cloud</strong></li><li>Build calibration from standards, then <strong>analyze and quantitate</strong> samples</li><li><strong>Start</strong>, <strong>stop</strong>, and <strong>edit your analysis remotely</strong> from anywhere in the world</li><li><strong>Access</strong> your instrument &amp; data <strong>via PC, tablet or mobile phone</strong></li><li><strong>Only 2 cables</strong> need – power and Ethernet.</li></ul><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/SIELC_Alltesta_Cromite_Analyzer_39037f4c54.jpg" alt="SIELC: Alltesta™ Cromite Analyzer."></p><h4><strong>Examples of Alltesta™-based methods</strong></h4><ul><li><a target="_blank" rel="noopener noreferrer" href="https://chrom.app/share/#/samples/12579/injection/22619?t=3f67e3c308cc479085c836cc682e8f1a"><strong>HPLC Method of Analysis of Ascorbic Acid</strong></a></li><li><a target="_blank" rel="noopener noreferrer" href="https://chrom.app/share/#/samples/12533/injection/22589/?t=43cad31c0e924440a18874658565c3db"><strong>HPLC Method of Analysis of Theobromine, Theophylline, Caffeine and C7</strong></a></li><li><a target="_blank" rel="noopener noreferrer" href="https://chrom.app/share/#/samples/12411/injection/22415/?t=0c93f4eb855c43aebcfd4c3b1fd55ce9"><strong>HPLC Method of Analysys of Aspirine</strong></a></li><li><a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/labrulez-bucket-strapi-h3hsga3/HPLC_Method_for_Analysis_of_Phenylephrine_Hydrochloride_1_in_Equate_Nasal_Spray_on_Primesep_100_Column_02f2983c25.pdf"><strong>HPLC Method for Analysis of Phenylephrine Hydrochloride 1% in Equate – Nasal Spray</strong></a></li><li><a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/labrulez-bucket-strapi-h3hsga3/HPLC_Method_for_Analysis_of_Rockstar_Zero_Carbs_Energy_Drink_with_Caffeine_and_Taurine_on_Primesep_100_Column_c87a85a0b4.pdf"><strong>HPLC Method for Analysis of Rockstar Zero Carbs Energy Drink with Caffeine and Taurine</strong></a></li></ul><h5><strong>Specifications</strong></h5><ul><li>Power Requirements → 110-250 VAC</li><li>Current Requirements → Up to 0.5 A</li><li>Dimensions (WHL) → 115 x 190 x 115 mm / 4.5 x 7.5 x 4.5 in</li><li>Weight → 1.45 kg / 3.15 lb</li><li>Sampling Rate → 20 Samples per second</li><li>Noise Level → Less than 0.05 mAU</li><li>Sync Time → Chromatogram is synced with HPLC Cloud every second</li><li>Operating Temperature → 0 to 40° C, non-condensing</li></ul><h4><strong>Connectors</strong></h4><ul><li>Standard DC 5V (5521) power plug connector</li><li>CAT5 ethernet connector for communication with the software</li></ul>

SIELC Alltesta Mini HPLC Autosampler

The Alltesta™ Mini Autosampler is an affordable and dependable solution for automated sample handling in various analytical and liquid handling applications, including HPLC.

<p><strong>The Alltesta™ Mini Autosampler is an affordable and dependable solution for automated sample handling in various analytical and liquid handling applications, including HPLC. With its proprietary alpha/beta mechanical design, this compact autosampler offers direct and comprehensive control of its features, making integration quick and easy. Users can tailor the mini autosampler to their needs with customizable valve configurations, tray designs, and syringe volumes and pressures. Additionally, a built-in shaking feature enables sample mixing before injection and/or simple sample extraction within the vial.</strong></p>
<p>This compact autosampler comes designed with original equipment manufacturer (OEM) customization in mind. Custom shape, color, and communication interfaces can be adapted to integrate with any system. Comprehensive software integration support is provided. Other options include metal-free sampling and reagent addition and mixing capabilities.</p>
<ul>
<li><p><strong>FRACTION COLLECTION CAPABILITY:</strong> A simple software modification can quickly convert this small autosampler into a fraction collector.</p>
</li>
<li><p><strong>REACTOR PROBE SAMPLER CAPABILITY:</strong> A simple software modification can quickly convert this small autosampler into a reactor probe sampler.</p>
</li>
</ul>
<p>This miniature autosampler can also be used as a component of our <a href="https://lcms.labrulez.com/products/1242"><strong>Alltesta™ Gradient Automated Analyzer</strong></a> and <a href="https://lcms.labrulez.com/products/1243"><strong>Alltesta™ Cromite Analyzer</strong></a>.</p>
<blockquote>
<h4><strong>The price of the Alltesta™ Mini Autosampler varies depending on the specific configuration requested.</strong></h4>
</blockquote>
<h4><strong>Specifications</strong></h4>
<ul>
<li>Dimensions (WHL): 160 x 145 x 195 mm / 6.3 x 5.71 x 7.68 in</li>
<li>Weight: 3 kg / 6.6 lb</li>
<li>Power: 24 V</li>
<li>Capacity: 48 vials or 96-well plate</li>
<li>Pressure: 5000 psi</li>
<li>Needle Wash: Inside and outside</li>
<li>Syringe Capacity: 120µL high-pressure / 4000µL low-pressure stainless / 4000µL low-pressure ceramic</li>
<li>Valve options: 6×2, 7×6 and 10×9 (more details here).</li>
<li>Volume Accuracy: 0.1µL</li>
<li>Communication: RS232 Virtual COM port via USB</li>
</ul>
<p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/SIELC_Alltesta_Mini_Autosampler_A_very_simple_schematic_operation_of_the_Alltesta_Autosampler_b79b0ad187.jpg" alt="SIELC: Alltesta™ Mini Autosampler - A very simple schematic operation of the Alltesta™ Autosampler."/></p>
<h4><strong>Software</strong></h4>
<p>The Alltesta™ Autosampler can be controlled:</p>
<ul>
<li>directly via serial commands (Alltesta™ protocol available upon request, please contact us at support@sielc.com),</li>
<li>directly via our demo software, allowing to learn the whole functionality of the unit (<a href="https://sielc.com/wp-content/uploads/2022/12/Alltesta_Autosampler_Control_Windows_10.zip"><strong>Windows 10, 32/64bit</strong></a>, 5Mb; <a href="https://sielc.com/wp-content/uploads/2022/12/Alltesta_Autosampler_Control_Windows_7.zip"><strong>Windows 7, 32/64bit</strong></a>, 126Mb).</li>
<li>remotely through our cloud software (as a part of Alltesta™ Analyzer or Alltesta™ Mini Analyzer),</li>
</ul>
<p>Serial communication drivers are available <a href="https://ftdichip.com/drivers/vcp-drivers/"><strong>here</strong></a>.</p>


SIELC Alltesta Mini High-Pressure Switching HPLC Valve

The Alltesta™ Mini High-Pressure Switching Valve offers versatility and convenience for laboratory fluid management applications.

<p><strong>The Alltesta™ Mini High-Pressure Switching Valve offers versatility and convenience for laboratory fluid management applications. It features flexible flow switching at varying flow rates and can be used independently or as part of the <a href="https://lcms.labrulez.com/products/124">Alltesta™ Analyzer</a> or <a href="https://lcms.labrulez.com/products/1244">Alltesta™ Autosampler</a>.</strong></p>
<p>With customizable options, including up to 10 available ports on the stator, the valve can be tailored to specific needs. It is built to handle pressures up to 5000 psi (350 bar) and boasts a fast 60 degree switch time of 200 milliseconds.</p>
<h4><strong>Specifications</strong></h4>
<ul>
<li>Power requirements: USB type B</li>
<li>Dimensions (WHL): 63 x 46 x 128 mm / 2.48 x 1.81 x 5.04 in</li>
<li>Weight: 0.45 kg / 0.95 lb</li>
<li>Switching speed for 60 degree: 200 ms</li>
<li>Max pressure: 350 bar (5000 psi)</li>
<li>Ports style: 10-32 thread with conical 1/16″ tube compression type seal</li>
<li>Materials in contact with liquid: coated SS316, Vespel® or PEEK or PTFE</li>
</ul>
<h4><strong>Available Configurations</strong></h4>
<ul>
<li>6x2 Valve</li>
<li>7x6 Valve</li>
<li>10x9 Valve</li>
</ul>
<p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/SIELC_SIELC_Alltesta_Mini_High_Pressure_Switching_Valve_Available_Configurations_72e3b13ea2.jpg" alt="SIELC: SIELC Alltesta™ Mini High-Pressure Switching Valve - Available Configurations."/></p>
<h4><strong>Software</strong></h4>
<p>The Alltesta™ High-Pressure Switching Valve can be controlled:</p>
<ul>
<li>directly via serial commands (option with greatest flexibility, Alltesta™ protocol available upon request, please contact us at support@sielc.com),</li>
<li>directly via our software, allowing to use the whole functionality of the unit without the need for automation (<a href="https://sielc.com/wp-content/uploads/2022/12/Alltesta_Valve_Control_Windows_10.zip"><strong>Windows 10, 32/64bit</strong></a>, 6 Mb, <a href="https://sielc.com/wp-content/uploads/2022/12/Alltesta_Valve_Control_Windows_7.zip"><strong>Windows 7, 32/64 bit</strong></a>, 127 Mb),</li>
<li>remotely through our cloud software (as a part of <a href="https://lcms.labrulez.com/products/1242"><strong>Alltesta™ Analyzer</strong></a>)</li>
<li>operator may also simply press the button on the front of the device to switch it to the next position</li>
</ul>
<p>Serial Communication Drivers are available <a href="https://ftdichip.com/drivers/vcp-drivers/"><strong>here</strong></a>.</p>
<h4><strong>Available Custom Options</strong></h4>
<ul>
<li>A High-flow version of the valve (preparative) is available</li>
<li>A metal-free version of the valve is also available.</li>
</ul>
<h4><strong>Valve Rotor Position Indication</strong></h4>
<p>LED color indicates the valve’s rotor position. By pressing the LED, the rotor can be advanced by one position. Depending on the valve configuration, the second press will either advance the rotor forward or backward. Position 1 – LED Green Position 2 – LED Navy Position 3 – LED Purple Position 4 – LED Blue Position 5 – LED Red Position 6 – LED White</p>
<h4><strong>Applications</strong></h4>
<ul>
<li>Column Switching</li>
<li>Solvent Selection</li>
<li>Sample Injection</li>
<li>Sample Cleaning</li>
</ul>


SIELC Alltesta™ Mini LHS (Liquid Handling Station)

The Alltesta™ Mini Liquid Handling Station serves as an affordable, reliable solution for automated sample handling.

<p><strong>The Alltesta™ Mini Liquid Handling Station serves as an affordable, reliable solution for automated sample handling. With its compact design, proprietary alpha/beta mechanical structure, and adjustable operation speeds, it offers comprehensive control for quick and seamless integration with other equipment. The station comes equipped with a built-in sample shaking feature for in-vial sample mixing and extraction, and a needle washing station capable of accommodating up to four solvents.</strong></p>
<p>Designed with Original Equipment Manufacturer (OEM) customization in mind, the Alltesta™ Mini Liquid Handling Station can be tailored to fit any system’s requirements. Custom needle lengths and trays enable the device to accommodate various types of vials. An array of integrated valves and a measuring syringe can be added to the device. Additional customization options, such as metal-free flow paths and customer-specified enclosure colors, are also available.</p>
<p>Comprehensive software integration support is provided to ensure smooth operation and functionality.</p>
<h4><strong>Specifications</strong></h4>
<ul>
<li>Dimensions (WHL): 160 x 145 x 195 mm / 6.3 x 5.71 x 7.68 in</li>
<li>Weight: 2.25 kg / 5 lb</li>
<li>Power: 24 V</li>
<li>Capacity: 48-vial tray or 96-well plate</li>
<li>Needle Wash: Inside and Outside</li>
<li>Communication: RS232 Virtual COM port via USB</li>
</ul>
<h4><strong>Software</strong></h4>
<p>The Alltesta™ LHS can be fully controlled via serial commands. Alltesta™ protocol available upon request, please contact us at support@sielc.com. Serial communication drivers are available <a href="https://ftdichip.com/drivers/vcp-drivers/"><strong>here</strong></a>.</p>


SIELC Alltesta Mini Syringe Pump

Alltesta™ Ultra High Pressure Mini Syringe Pump is designed to produce a steady, high pressure, pulsation-free flow, so there is no need for bulky dampeners, mixers, and precision check valve arrangements.

<p><strong>Alltesta™ Ultra High Pressure Mini Syringe Pump is designed to produce a steady, high pressure, pulsation-free flow, so there is no need for bulky dampeners, mixers, and precision check valve arrangements. It can refill prior to each run in less than a minute, and can produce flow rates from as low 1 μL/min to as fast as 4000 μL/min with pressure up to 4000 psi (285 bar).</strong></p>
<p>The Mini Syringe Pump allows for several communication options via its USB/Serial port. Just like the rest of our Alltesta™ line of devices, our serial protocol allows to control and automate every function of the device. We also provide free Windows-based software allowing full control of the device.</p>
<h4><strong>Specifications</strong></h4>
<ul>
<li>Syringe volume: 5.8 mL</li>
<li>Power Requirements 24 VDC</li>
<li>Current Requirements Up to 1.5 A</li>
<li>Dimensions (WHL) 63x167x128 mm / 2.48×6.57×5.04 in</li>
<li>Weight 1.65 kg / 3.7 lb</li>
<li>Flow Rate Range 0.001 to 4 mL/min</li>
<li>Speed Accuracy 1% of full-scale</li>
<li>Linearity Full Range 0.001 to 4.0 mL/min</li>
<li>Pressure 4000 psi (285 bar) continuous</li>
<li>Pressure accuracy ±1 psi</li>
<li>Operating Temperature 0 to 40° C, non-condensing</li>
<li>Inlet/Outlet Thread 10-32 UNF compression type</li>
<li>Materials in contact with liquid SS316, PTFE, PEEK</li>
<li>Communication USB virtual serial port</li>
</ul>
<h4><strong>Software</strong></h4>
<p>The Mini Syringe Pump can be controlled:</p>
<ul>
<li>directly through your software via serial commands (Alltesta™ protocol available upon request, please contact us at support@sielc.com),</li>
<li>remotely through our cloud software (as a part of <a href="https://lcms.labrulez.com/products/1242"><strong>Alltesta™ Gradient Automated Analyzer</strong></a> and <a href="https://lcms.labrulez.com/products/1243"><strong>Alltesta™ Cromite Analyzer</strong></a>.),</li>
<li>directly via our demo software, allowing to learn the whole functionality of the unit (<a href="https://sielc.com/wp-content/uploads/2022/12/Alltesta_Pump_Control_Windows_10.zip"><strong>Windows 10, 32/64bit</strong></a>, 5Mb; <a href="https://sielc.com/wp-content/uploads/2022/12/Alltesta_Pump_Control_Windows_7.zip"><strong>Windows 7, 32/64bit</strong></a>, 126Mb)</li>
</ul>
<p>Serial Communication Drivers are available <a href="https://ftdichip.com/drivers/vcp-drivers/"><strong>here</strong></a>.</p>
<h4><strong>Available Custom Options</strong></h4>
<p>Metal-free version of the pump includes no metal contact pump head and can be operated up to 4000 psi.</p>


SIELC Alltesta Mini UV-Vis Detector

The Alltesta™ UV-Vis Multi-wavelength Mini Detector is a compact and versatile device that can continuously monitor the optical properties of solutions flowing through it.

<p><strong>The Alltesta™ UV-Vis Multi-wavelength Mini Detector is a compact and versatile device that can continuously monitor the optical properties of solutions flowing through it. The Mini Detector is ideal for use in HPLC, Flow Injection Analysis (FIA), and preparative and flash chromatography.</strong></p>
<p>The device comes standard with four different wavelength channels, including 470 nm (Blue), 520 nm (Green), 630 nm (Red), and a choice between 255 nm or 275 nm for the UV channel. The Mini Detector features a simple setup process with a USB interface that allows it to communicate with the <a href="https://lcms.labrulez.com/products/1242"><strong>Alltesta™ Gradient Automated Analyzer</strong></a> or <a href="https://lcms.labrulez.com/products/1243"><strong>Alltesta™ Cromite Analyzer</strong></a> or integrate with other instrumentation.</p>
<p>Powered solely by its USB port, this mini detector is capable of monitoring multiple analytes simultaneously. It has a low optical cell volume of &lt; 3 µL, which results in minimal dispersion even at low flow rates, and can collect data at rates of up to 20 sps for all four channels, making it suitable for demanding applications. The Mini Detector boasts low noise and linearity over more than 4 orders of magnitude.</p>
<p>In addition to its compact size and versatility, the Mini Detector also has zero warm-up time and exceptional durability. The detector cell volume is available in two sizes: 3 μL and 10 μL.</p>
<h4><strong>Specifications</strong></h4>
<ul>
<li>Dimensions (WHL): 63 x 46 x 116 mm / 2.48 x 1.81 x 4.57 in</li>
<li>Weight: 0.25 kg / 0.55 lb</li>
<li>Sample Cell Volume: &lt; 3µL</li>
<li>Cell Optical Path: 7.0mm</li>
<li>Liquid Ports: 10-32 UNF</li>
<li>Wavelength Range: 250nm &amp; up</li>
<li>Simultaneous Wavelengths: Up to 4</li>
<li>Data Rate: 20 sps</li>
<li>Noise Level: 0.03 mAU</li>
<li>Power: USB Type B</li>
</ul>
<h4><strong>Software</strong></h4>
<p>The Multi-wavelength Detector can be controlled:</p>
<ul>
<li>directly via serial commands (Alltesta™ protocol available upon request, please contact us at support@sielc.com),</li>
<li>directly via our demo software, allowing to learn the whole functionality of the unit (<a href="https://sielc.com/wp-content/uploads/2022/12/Alltesta_Detector_Control_Windows_10.zip"><strong>Windows 10, 32/64bit</strong></a>, 5Mb, <a href="https://sielc.com/wp-content/uploads/2022/12/Alltesta_Detector_Control_Windows_7.zip"><strong>Windows 7, 32/64bit</strong></a>, 127Mb)</li>
<li>remotely through our cloud software (as a part of <a href="https://lcms.labrulez.com/products/1242"><strong>Alltesta™ Gradient Automated Analyzer</strong></a> and <a href="https://lcms.labrulez.com/products/1243"><strong>Alltesta™ Cromite Analyzer</strong></a>).</li>
</ul>
<p>Serial Communication Drivers are available <a href="https://ftdichip.com/drivers/vcp-drivers/"><strong>here</strong></a>.</p>


Products distributed by LabRulez - page 4

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