<div>Purifying the target protein is crucial for investigating its specific properties and interactions with biomolecules like DNA, RNA, and other proteins. A range of purification strategies is available, and the optimal approach depends on factors such as the required scale, throughput, and downstream application. In many cases, the process must be fine-tuned through empirical optimization to achieve the desired purity and yield.</div><div><div><div><div><h3>What is Protein Purification?</h3>Protein purification is a critical process in biotechnology and life sciences, aimed at isolating a specific protein from complex biological mixtures such as cell lysates or tissue extracts. The goal is to obtain a highly purified protein sample, free from contaminants like other proteins, nucleic acids, and lipids, to enable accurate analysis of its structure, function, and interactions.This process is especially vital in biotechnological areas inside immunology and microbiology. In immunology, it underpins the production of vaccines, therapeutic antibodies, and diagnostic assays by enabling the isolation of key immune proteins and antibodies. It also supports the investigation of cell signaling and immune response mechanisms. In microbiology, purified proteins are essential for studying pathogens, identifying disease-related antigens, and driving drug discovery efforts. Ultimately, protein purification is fundamental to the development of targeted vaccines, innovative diagnostics, and effective therapeutic strategies.</div></div></div></div><div><div><div><div><h3>Types of Protein Purification Methods</h3></div></div></div></div><div>There are several types of protein purification methods, each chosen based on the protein’s characteristics such as size, charge, solubility, and binding affinity. Below are some commonly used methods:</div><div><div><div><div><h4>Non-Chromatographic Approaches</h4></div></div><div>Non-chromatographic approaches use simpler physicochemical principles—such as solubility, molecular size, or diffusional properties—to separate and purify proteins. These techniques are often employed as initial purification or concentration steps before further refinement.<h5>Precipitation Techniques</h5>Protein precipitation is a widely used approach in purification workflows, typically relying on one of three main methods:<ul><li>Organic solvent precipitation involves adding solvents like ethanol or acetone to lower the solution's dielectric constant. This promotes protein–protein interactions, reducing solubility and leading to aggregation and precipitation.</li><li>Isoelectric point precipitation exploits the pH at which a protein carries no net charge. At this point, solubility is minimized, causing the protein to precipitate from solution.</li><li>Nonionic polymer precipitation uses polymers such as dextran, polyethylene glycol (PEG), or NPEP to alter the solution’s physical properties. These agents create crowding effects that promote protein aggregation without directly binding to the proteins.</li></ul><h5>Dialysis</h5>Dialysis involves placing a protein solution inside a semi-permeable membrane that allows water and ions to pass while the protein remains inside, facilitating buffer exchange.This method is commonly used during protein expression and purification workflows for buffer exchange, desalting, and removal of unwanted small molecules such as imidazole, urea, or ammonium sulfate. The process helps maintain protein stability and activity by gradually equilibrating the sample with the desired buffer conditions.<h5>Immunocapture (Magnetic Bead-Based Purification</h5>Immunocapture uses antibodies immobilized on solid supports like magnetic beads to selectively bind and isolate a target protein. This method is highly specific and particularly useful for low-abundance targets or small-scale prep, such as:<ul><li>Pulling down proteins from complex mixtures</li><li>Capturing tagged recombinant proteins (e.g., FLAG, HA, or His tags)</li><li>Enrichment before western blotting, MS, or ELISA</li></ul>Magnetic beads offer speed, ease of use, and minimal sample loss, making them ideal for lab-scale workflows.<a target="_blank" rel="noopener noreferrer" href="https://www.phenomenex.com/products/biozenspe-sample-preparation-products/biozen-magbeads">Biozen MagBeads</a> provide a fast and efficient method for purifying, isolating, and cleaning up monoclonal antibodies (mAbs), proteins, and peptides. These paramagnetic beads feature a streptavidin-coated surface, enabling high-affinity binding in immuno-based workflows. Compared to traditional sample preparation techniques, magnetic bead-based immunocapture offers greater speed and consistency by delivering high binding capacity with uniform particle size, ensuring accurate and reproducible results in significantly less time.<h5>Centrifugation</h5>Centrifugation of crude protein mixtures is an essential step in the investigation of protein purification and aggregation. Outlined below are the various types of centrifugations employed in protein purification.Preparative Ultracentrifugation Ultracentrifugation is an effective method of purifying proteins from aggregates based on their size, shape and density. Centrifugation, widely recognized as a robust and versatile method, is commonly used to separate complex mixtures of cellular components. Ultracentrifuges separate small particles by spinning at high speeds, utilizing centrifugal force based on size and density.Differential Centrifugation Differential centrifugation operates by separating components based on differences in size and density, using varying speeds of centrifugation to achieve this. Its resolving power ranges from low to medium. A key advantage of this technique is its convenience for processing multiple samples simultaneously, making it effective for purifying crude samples. Additionally, it preserves cell viability and maintains the natural distribution of hematopoietic cell populations.Rate-zonal Gradient Centrifugation This technique separates components based on their sedimentation rate within a medium that contains a density gradient. It offers medium resolving power and is particularly effective for separating multiple components in complex mixtures with high precision. It is also considered a cost-effective and widely used technique in molecular biology and biochemistry.Isopycnic Centrifugation (Equilibrium Centrifugation) Isopycnic centrifugation is an advanced protein purification method that relies on the principle of separating particles according to their intrinsic buoyant density, irrespective of their size or morphology. During the process, proteins migrate through a density gradient until they equilibrate at a position where their density matches that of the surrounding medium, resulting in precise separation without further sedimentation.</div></div></div><div><div><div><div><h4>Chromatographic Approaches</h4>Chromatography is a common method of protein purification and is based on the separation of molecules in a mixture as they move through a stationary phase and a mobile phase. Outlined below are the various types of chromatography employed in protein purification.<h5>Ion Exchange Chromatography</h5><a target="_blank" rel="noopener noreferrer" href="https://www.phenomenex.com/techniques/hplc-ion-exchange">Ion exchange chromatography</a> relies on the interaction between a charged stationary phase and oppositely charged analytes, with cation exchange chromatography attracting positively charged molecules to a negatively charged stationary phase. Successful application of this technique requires careful selection of the appropriate ion exchange column, buffer pH, and salt concentration. This approach is often used in the biopharmaceutical industries as mAb polishing steps, viral clearance and charge variant analyses as part of the product quality control.<a target="_blank" rel="noopener noreferrer" href="https://www.phenomenex.com/">Phenomenex</a> offers a range of protein purification columns. Their ion exchange chromatography solutions include:&nbsp;<ul><li><a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/products/1526">Luna SCX </a>for strong selectivity of positively charged compounds, such as peptides</li><li><a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/products/1540">Biozen WCX</a> for efficient separation of proteins such as monoclonal antibodies charged variants</li><li><a target="_blank" rel="noopener noreferrer" href="https://www.phenomenex.com/part/00g-3151-e0">PhenoSphere SAX</a>, a strong anion-exchanger with fully porous 80Å particles, particularly useful for small molecules such as nucleotides</li></ul><h5>Size Exclusion Chromatography</h5><a target="_blank" rel="noopener noreferrer" href="https://www.phenomenex.com/techniques/hplc-sec">Size exclusion chromatography (SEC)</a> separates molecules based on their size, with larger analytes eluting first due to their exclusion from the pores in the column packing material, while smaller molecules are able to enter the pores, facing a longer travelling path, and hence, eluting later. This method can be categorized into aqueous SEC (gel filtration chromatography, GFC) for analyzing biomolecules such as proteins and antibodies. This type of chromatography is widely used as an analytical tool for quality control in the biopharmaceutical industry and can also be an approach of choice for polishing steps.Phenomenex offers a range of Biozen columns for SEC separations. In the case of mAbs, <a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/products/1534">Biozen dSEC-2</a> and<a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/products/1537"> Biozen dSEC-7 </a>columns are the top choices for monoclonal antibody (mAb) analysis, delivering high-resolution separation of monomers, aggregates, and fragments formed during mAb manufacturing, processing, or storage. The dSEC-7 column, featuring a larger pore size and optimized particle design, extends the dynamic range to accommodate larger biomolecules and complex biotherapeutics.For more general applications, <a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/products/1533">Biozen SEC-2</a> and <a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/products/1532">Biozen SEC-3</a> phases cover different MW ranges for high-efficiency separation of biomolecules (e.g., proteins and antibodies). Additionally, the BioSep and Yarra lines become top choices when looking for preparative scale columns.<h5>Reversed-Phase Chromatography</h5><a target="_blank" rel="noopener noreferrer" href="https://www.phenomenex.com/techniques/hplc-reversed-phase">Reversed-phase chromatography</a>, the most common mode in high-performance liquid chromatography/ ultra-high-performance liquid chromatography (HPLC/UHPLC), separates compounds based on hydrophobic and van der Waals interactions using alkyl (C18, C8, C4) stationary phases or other hydrophobic polymers. For large biomolecules such as proteins and peptides, <a target="_blank" rel="noopener noreferrer" href="https://www.phenomenex.com/resources/blog/wider-pore-biozen-column">wide-pore</a> (typically 300 Å) C4, C8 and C18 columns are ideal, as the larger pore size allows better accessibility of the protein molecules to the stationary phase surface, improving mass transfer and peak shape during separation.The Biozen line from Phenomenex offers a wide range of HPLC/UHPLC columns for the analysis of biomolecules.These include phases optimized for high-resolution analysis of intact proteins (Native-RP, Widepore C4, Intact XB-C8) and peptides (Peptide PS-C18, Peptide XB-C18 and Peptide Polar-C18). Kinetex could also be an option when looking for more selectivities for the analysis of peptides and small proteins. Additionally, when aiming to preparative scale processes, several of these media are also available in Phenomenex Axia formats to easily up-scale your separations.<h5>Hydroxyapatite Chromatography</h5>Hydroxyapatite chromatography (HAC) is a sophisticated mixed-mode chromatographic technique that exploits the unique properties of hydroxyapatite (Ca10(PO4)6(OH)2), utilizing both cation-exchange interactions via its phosphate groups and metal affinity through its calcium sites to achieve highly selective protein separations.Renowned for its efficacy in resolving complex biomolecular separations, HAC excels in tasks like aggregate removal from monoclonal antibodies, surpassing the capabilities of traditional single-mode chromatographic methods such as ion-exchange or hydrophobic interaction chromatography.<h5>Hydrophobic Interaction Chromatography</h5>Hydrophobic interaction chromatography (HIC) is a method of protein purification that separates molecules based on their hydrophobicity, using high-salt buffers to promote protein-ligand interactions while maintaining the protein's biological activity. The process involves gradually reducing the salt concentration to elute proteins in order to increase hydrophobicity, making it effective for purifying target molecules and removing impurities or aggregates, making it a valuable approach for intermediate and polishing steps during purification processes.<h5>Affinity Chromatography</h5>Affinity chromatography is a powerful and highly selective technique used to purify biomolecules based on specific, reversible interactions between a target molecule and an immobilized ligand. In many cases, recombinant proteins are engineered with affinity tags—such as His-tags or GST-tags—that facilitate binding to complementary ligands on the chromatography matrix.This method offers the advantage of achieving several thousand-fold purifications in a single step while preserving the biological activity of the protein. It is widely employed for purifying enzymes, antibodies, and antigens, using a variety of ligands including specific antibodies, enzymes, and multifunctional compounds like concanavalin A and triazine dyes.</div></div></div></div>

Exploring Protein Purification Methods: Essential Techniques for Accurate Results

<h3>A Practical Guide to Getting the Most from Syringe Filters: Tips, Tricks, and Common Pitfalls</h3>Syringe filters play a crucial role in chromatography and many other laboratory applications by removing particulate matter before analysis. Proper sample filtration helps protect analytical instruments, extends column lifetime, and improves data quality. However, selecting an unsuitable filter—or using one incorrectly—can introduce contamination, reduce filtration efficiency, or even compromise analytical results. The following recommendations will help you avoid common mistakes and ensure reliable filtration every time.<img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/KNAUER_Avoiding_Common_Syringe_Filter_Mistakes_3_150e8c1c2b.webp" alt="KNAUER: Avoiding Common Syringe Filter Mistakes" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_KNAUER_Avoiding_Common_Syringe_Filter_Mistakes_3_150e8c1c2b.webp 133w," sizes="100vw" width="1276" height="1496"><h4>1. Improper Filter Handling</h4>Using excessive force during filtration or attempting to reuse disposable syringe filters can damage the membrane and increase the risk of sample contamination.<ul><li>Solution: Apply slow, even pressure throughout the filtration process and always dispose of single-use filters after one use. If the flow rate decreases noticeably, replace the filter instead of applying additional force.</li></ul><h4>2. Selecting an Inappropriate Pore Size</h4>Using the wrong pore size may either fail to remove unwanted particles or cause the membrane to clog prematurely, reducing filtration efficiency.<ul><li>Solution: A 0.45 µm membrane is generally suitable for routine particulate removal, while a 0.22 µm membrane is recommended for finer filtration and bacterial removal. Samples with higher viscosity may require a pre-filter to prevent rapid clogging. It is also important to consider the intended analytical technique. For example, 0.22 µm filters are typically preferred for UHPLC and LC-MS applications, whereas 0.45 µm filters are often sufficient for conventional HPLC analyses.</li></ul><h4>3. Choosing the Wrong Membrane Material</h4>Different membrane materials exhibit different chemical resistance. Using an incompatible membrane may lead to degradation of the filter, sample contamination, or complete filtration failure.<ul><li>Solution: Always verify solvent compatibility before filtration. PTFE membranes are an excellent choice for organic solvents, while PES and nylon membranes are better suited to aqueous samples. If compatibility is uncertain, perform a small-scale trial before filtering valuable samples.</li><li>Pro Tip: For additional guidance, see our blog article "How to Choose the Right Membrane for your Syringe Filter", which provides a detailed comparison of membrane materials and their recommended applications.</li></ul><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/KNAUER_Avoiding_Common_Syringe_Filter_Mistakes_2_e47021fb11.jpg" alt="KNAUER Sepapure® Syringe Filters are available with multiple membrane materials, each identified by a dedicated color code for quick and reliable selection." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_KNAUER_Avoiding_Common_Syringe_Filter_Mistakes_2_e47021fb11.jpg 245w," sizes="100vw" width="1580" height="902"><h4>4. Ignoring Filter Capacity</h4>Using a filter with insufficient capacity can result in rapid membrane blockage, increasing filtration time and reducing laboratory productivity.<ul><li>Solution: When filtering larger sample volumes, choose high-capacity syringe filters or introduce a pre-filtration step. Never force liquid through a clogged membrane, as doing so may damage the filter and negatively affect sample quality.</li></ul><h4>5. Overlooking Sample Adsorption</h4>Certain membrane materials may adsorb analytes of interest, leading to lower recoveries and potentially affecting quantitative results.<ul><li>Solution: For protein samples or other adsorption-sensitive analytes, select membranes with low binding characteristics, such as nylon or glass fiber, whenever appropriate. Performing a small recovery test before routine analysis can help identify potential analyte losses.</li></ul><h4>6. Failing to Optimize Filtration Performance</h4>Slow filtration is more than just an inconvenience—it often indicates that the filtration conditions are not optimal.<ul><li>Solution: If filtration becomes unusually slow, investigate possible causes such as membrane clogging, excessive sample viscosity, or an unnecessarily small pore size. Depending on the sample, dilution or sequential filtration using progressively finer filters may significantly improve performance.</li></ul><h4>Final Thoughts</h4>Selecting the appropriate syringe filter is a straightforward process, but overlooking small details can have a significant impact on analytical performance. Choosing the correct membrane material, pore size, and filtration strategy helps improve laboratory efficiency, protects chromatography systems and columns, and contributes to accurate, reproducible analytical results.With the right syringe filter and proper filtration technique, you can minimize sample preparation problems and maximize confidence in every analysis.<img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/KNAUER_Avoiding_Common_Syringe_Filter_Mistakes_4_983a15e289.jpg" alt="KNAUER: Sepapure® Syringe Filter Selection" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_KNAUER_Avoiding_Common_Syringe_Filter_Mistakes_4_983a15e289.jpg 245w," sizes="100vw" width="1160" height="627">Happy filtering!

Avoiding Common Syringe Filter Mistakes

News from LabRulezLCMS Library - Week 27, 2026

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Everything from the world of analytical chemistry in one place. We connect people in solving their problems. At Labrulez you will find all the necessary information easily, quickly and clearly. Stop searching and start finding.

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Phenomenex is a global technology leader committed to developing novel analytical chemistry solutions that solve the separation and purification challenges of researchers in industrial, government and academic laboratories.

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Based in Berlin, KNAUER is a medium-sized, owner-managed company that has been serving the sciences since 1962. We develop and manufacture scientific instruments of superior quality for liquid chromatography, including: Analytical HPLC/UHPLC, Preparative HPLC, Fast protein liquid chromatography (FPLC), Multi-column chromatography/Simulated moving bed (SMB) chromatography. High pressure dosing, in-line detection systems, and osmometry are additional business areas.

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Thermo Scientific Accucore aQ C18 Polar Endcapped HPLC Columns

Separate polar compounds with improved speed and resolution using Thermo Scientific™ Accucore™ aQ C18 Polar Endcapped LC columns.

Separate polar compounds with improved speed and resolution using Thermo Scientific™ Accucore™ aQ C18 Polar Endcapped LC columns. Rugged 2.6μm solid-core particles enable fast, high-efficiency separations at low backpressures.The polar endcapped C18 stationary phase provides optimal retention of polar analytes and is fully compatible with 100% aqueous mobile phases. Robust bonding technology and automated packing procedures ensure excellent reproducibility and long column lifetimes.<h5>Alternative Selectivity</h5><ul><li>Polar endcapped C18 stationary phase provides optimum retention of polar analytes</li><li>Accucore aQ Columns are compatible with highly aqueous mobile phases.</li><li>Polar encapping allows use in 100% aqueous mobile phases without loss of performance or stability.</li></ul><h5>Fast Separations</h5><ul><li>2.6μm core-enhanced technology particles enable operation at high flow rates without generating excessive backpressures.</li></ul><h5>Excellent Peak Shapes for Enhanced Resolution and Sensitivity</h5><ul><li>Solid-core 2.6μm particles and narrow particle size distribution reduce band broadening and improve separation efficiencies.</li><li>High surface coverage of silica minimizes secondary interactions and peak tailing.</li></ul><h5>Low Backpressures</h5><ul><li>Solid-core 2.6μm particles for less backpressure compared to UHPLC sub-2μm particles</li><li>Compatible with conventional HPLC instrumentation and UHPLC platforms</li></ul><h5>Excellent Reproducibility and Robustness</h5><ul><li>Advanced bonding technology</li><li>Automated packing process</li></ul><h4>Specifications</h4><ul><li>Endcapped: Polar</li><li>Max. Pressure: 14500 psi (1000 bar)</li><li>Packing Material: Spherical, Solid Core, Ultrapure Silica</li><li>Particle Shape: Spherical Solid Core</li><li>Stationary Phase: C18</li><li>Surface Area: 130 m²/g</li><li>Temperature: 70°C</li><li>USP Type: L1</li><li>Carbon Load: 9%</li><li>Phase: Reversed Phase</li><li>Pore Size: 80 Å</li><li>pH: 2 to 9</li></ul>

Thermo Scientific Accucore Polar Premium HPLC Columns

Achieve fast, high-resolution separations of polar and nonpolar analytes using Thermo Scientific™ Accucore™ Polar Premium LC columns.

Achieve fast, high-resolution separations of polar and nonpolar analytes using Thermo Scientific™ Accucore™ Polar Premium LC columns. Rugged 2.6μm solid-core particles enable fast, high-efficiency separations at low backpressures.&nbsp;The robust amide-embedded C18 stationary phase provides unique selectivity complementary to conventional C18 columns, is stable from pH 1.5–10, and is fully compatible with highly aqueous mobile phases. Robust bonding technology and automated packing procedures ensure excellent reproducibility and long column lifetimes.<h5>Alternative Selectivity</h5><ul><li>Polar embedded reverse phase material offers complementary selectivity to standard C18 columns.</li></ul><h5>Stable over Wide pH Range</h5><ul><li>Specially designed bonded phase is stable from pH 1.5–10.5.</li></ul><h5>Compatible with Highly Aqueous Mobile Phases</h5><ul><li>Rugged amide-embedded C18 phase will not undergo phase collapse in 100% aqueous mobile phase.</li></ul><h5>Fast Separations</h5><ul><li>2.6μm core-enhanced technology particles enable operation at high flow rates without generating excessive backpressures.</li></ul><h5>Excellent Peak Shapes for Enhanced Resolution and Sensitivity</h5><ul><li>Solid-core 2.6μm particles and narrow particle size distribution reduce band broadening and improve separation efficiencies.</li><li>High surface coverage of silica minimizes secondary interactions and peak tailing.</li></ul><h5>Low Backpressures</h5><ul><li>Solid-core 2.6μm particles for less backpressure compared to UHPLC sub-2μm particles</li><li>Compatible with conventional HPLC instrumentation and UHPLC platforms</li></ul><h5>Excellent Reproducibility and Robustness</h5><ul><li>Advanced bonding technology</li><li>Automated packing process</li></ul><h4>Specifications</h4><ul><li>Endcapped: Yes</li><li>Max. Pressure: 14500 psi (1000 bar)</li><li>Packing Material: Spherical, Solid Core, Ultrapure Silica</li><li>Particle Shape: Spherical Solid Core</li><li>Stationary Phase: Polar Embedded</li><li>Surface Area: 90 m²/g</li><li>Temperature: 70°C</li><li>USP Type: L60</li><li>Carbon Load: 9%</li><li>Phase: Reversed Phase</li><li>Pore Size: 150 Å</li><li>pH: 1.5 to 10.5</li></ul>

Thermo Scientific Accucore Phenyl-Hexyl HPLC Columns

Offer unique selectivity for aromatic and moderately polar analytes with Thermo Scientific™ Accucore™ Phenyl-Hexyl LC Columns.

Offer unique selectivity for aromatic and moderately polar analytes with Thermo Scientific™ Accucore™ Phenyl-Hexyl LC Columns.Based on Core Enhanced Technology, Accucore columns provide fast, high-resolution separations without the elevated backpressures required by sub-2μm particles.<ul><li>Fast separations with excellent resolution and sensitivity</li><li>Lower backpressures so that UHPLC is not required</li><li>Mixed-Mode Selectivity for Aromatic and Moderately Polar Analytes</li><li>Enhanced Pi-pi Interactions with Aromatics</li><li>Moderate Hydrophobicity</li></ul><h4>Specifications</h4><ul><li>Endcapped: Yes</li><li>For Use With: HPLC</li><li>Max. Pressure: 14500 psi (1000 bar)</li><li>Packing Material: Spherical, Solid Core, Ultrapure Silica</li><li>Particle Shape: Spherical Solid Core</li><li>Stationary Phase: Phenyl/Hexyl</li><li>Surface Area: 130 m²/g</li><li>Temperature: 70°C</li><li>USP Type: L11</li><li>Carbon Load: 5%</li><li>Phase: Reversed Phase</li><li>Pore Size: 80 Å</li><li>pH: 2 to 8</li></ul>

Thermo Scientific Q Exactive Plus Hybrid Quadrupole-Orbitrap Mass Spectrometer

Thermo Scientific Q Exactive Plus Hybrid Quadrupole-Orbitrap Mass Spectrometer confidently and routinely characterize, quantify, and confirm in a single analysis using a single mass spectrometer.

Take Quanfirmation™ to the next level. The Thermo Scientific™ Q Exactive™ Hybrid Quadrupole-Orbitrap™ Mass Spectrometer introduced the revolutionary concept of Quanfirmation. The Q Exactive™ Plus Hybrid Quadrupole-Orbitrap™ Mass Spectrometer takes Quanfirmation to the next level with increased performance and new options that enhance applications from confident DMPK qual/quan screening studies to characterization of intact monoclonal antibodies.<h5>Superior Quantitation</h5><ul><li>Advanced quadrupole technology (AQT) improves precursor selection and transmission for more-accurate quantitation of low-abundance analytes in complex matrices</li><li>Sophisticated data-independent acquisition (DIA) and parallel reaction monitoring (PRM) deliver reproducible quantitation with complete qualitative confidence</li><li>An advanced active beam guide (AABG) reduces noise and extends maintenance intervals</li></ul><h5>Enhanced Analysis of Intact Proteins</h5><ul><li>Optional intact protein mode provides superior trapping of large molecules for improved analysis of intact proteins and protein complexes</li><li>Optional enhanced resolution ensures maximum ID confidence in top-down proteomics and lipidomics</li></ul><h5>High-Confidence Results</h5><ul><li>HR/AM and full-scan capabilities capture all sample data, all of the time, enabling retrospective data analysis without need to re-run samples</li><li>Resolving power up to 140,000 FWHM eliminates isobaric interferences, increasing confidence in results when analyzing samples in complex matrices</li><li>Better than 1-ppm mass accuracy in full- and all-ion fragmentation (AIF) scan modes ensures confident compound identification</li><li>Fast scanning at 12 Hz and spectral multiplexing suited to UHPLC applications</li><li>Rapid polarity switching maximizes information obtained</li><li>AIF and multiple dissociation techniques — in-source CID and HCD — aid in compound identification</li><li>Mass range to 6000 m/z enhances detection of singly charged small molecules and biomolecules</li><li>More than four orders of magnitude intrascan dynamic range, along with femtogram-level sensitivity, allow detection of trace-level and high-abundance compounds in the same scan</li></ul>

Thermo Scientific™ Q Exactive™ UHMR Hybrid Quadrupole Orbitrap™ Mass Spectrometer

Thermo Scientific Q Exactive UHMR Hybrid Quadrupole-Orbitrap MS offers ultra-high mass range native MS with top-down capabilities, extreme sensitivity, and resolution up to 80,000 m/z.

<h3>Go beyond what you thought possible with native mass spectrometry</h3>The Thermo Scientific™ Q Exactive™ UHMR is the first hybrid quadrupole-Orbitrap™ mass spectrometer designed specifically for ultra-high mass range (UHMR) native MS and top-down protein analysis. It combines exceptional sensitivity, ultra-high resolution at high m/z, and unique pseudo-MS³ capabilities, enabling detailed characterization of intact protein assemblies and complex biological systems.<h3>🔬 Breakthrough in Native Mass Spectrometry</h3>Native mass spectrometry preserves the non-covalent structure and interactions of proteins and protein complexes, making it an indispensable tool for structural biology and biopharma. The Q Exactive UHMR system enables direct mass analysis of intact macromolecules, including membrane proteins, virus capsids, and ribosomal particles—with unrivaled detail.<ul><li>Ultra-high mass range detection up to 80,000 m/z</li><li>Native top-down analysis of protein complexes and assemblies</li><li>High sensitivity for precious or low-abundance samples</li><li>Resolve mass differences even in heterogeneous megaDalton assemblies</li></ul><h3>⚙️ Technological Innovations</h3>The Q Exactive UHMR system integrates multiple hardware advancements to overcome the historical limitations of native MS:<ul><li>In-source trapping for efficient desolvation and controlled dissociation</li><li>Advanced quadrupole mass filter with selection up to 25,000 m/z</li><li>Reduced RF frequencies across the ion path for better high-mass transmission</li><li>Pseudo-MS³ capability: fragment large complexes into subunits, then sequence them in HCD cell</li></ul><h3>🧬 Applications and Capabilities</h3><h4>Confident characterization of complex biological systems:</h4><ul><li>Virus capsid analysis: Differentiation and sequencing of 3–4 MDa hepatitis B virus capsids</li><li>Ribosome studies: Resolution of substoichiometric proteins in E. coli 70S particles</li><li>Membrane proteins: Gentle liberation from micelles and native top-down sequencing (e.g., AmtB trimer)</li><li>Chaperonin complexes: Native top-down analysis of GroEL tetradecamer</li></ul><h4>Detect and resolve small differences in high-mass species:</h4><ul><li>Post-translational modifications</li><li>Ligand binding</li><li>Protein truncations and isoforms</li></ul><h3>💡 Software for Confident Data Interpretation</h3>Maximize the power of native MS with industry-leading software:<ul><li><a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/products/1837">BioPharma Finder™ Software </a>– For intact mass confirmation, top-down analysis, and PTM localization</li><li>ProSightPC™ Software – Advanced top-down proteomics with integrated UniProt database search</li></ul><h3>🧪 Who Is It For?</h3>Ideal for research groups and industry labs focused on:<ul><li>Structural biology</li><li>Biotherapeutic development</li><li>Native and top-down proteomics</li><li>Protein-ligand interaction studies</li></ul><h3>🧭 Summary of Key Features</h3><ul><li>Mass range: Up to 80,000 m/z</li><li>Quadrupole isolation: Up to 25,000 m/z</li><li>Sensitivity: Orders of magnitude higher at high m/z</li><li>Fragmentation: HCD, pseudo-MS3 for native top-down</li><li>In-source trapping: Yes, optimized for membrane protein assemblies</li><li>Software: BioPharma Finder, ProSightPC</li><li>Compatible analyses: Native MS, top-down, intact mass, charge detection</li></ul><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Pragolab_Thermo_Q_Exactive_UHMR_Hybrid_Quadrupole_Orbitrap_hmotnostni_spektrometr_a7f594a56d/Pragolab-Thermo-Q-Exactive-UHMR-Hybrid-Quadrupole-Orbitrap-hmotnostni-spektrometr.webp" alt="Thermo Scientific™ Q Exactive™ UHMR Hybrid Quadrupole Orbitrap™ Mass Spectrometer" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Pragolab_Thermo_Q_Exactive_UHMR_Hybrid_Quadrupole_Orbitrap_hmotnostni_spektrometr_a7f594a56d/thumbnail_Pragolab-Thermo-Q-Exactive-UHMR-Hybrid-Quadrupole-Orbitrap-hmotnostni-spektrometr.webp 156w," sizes="100vw" width="650" height="650">

Thermo Scientific™ Orbitrap™ IQ-X™ Tribrid™ Mass Spectrometer

Know your unknown compounds with confidence, using intelligent mass spectrometry that combines ease-of-use with smart acquisition methods to deliver higher data certainty.

Designed to unravel complex chemical structures while delivering the highest versatility and comprehensive workflow support for unknown compound ID and small molecule analysis, the Thermo Scientific™ Orbitrap™ IQ-X™ Tribrid™ mass spectrometer empowers you to collect more meaningful data.Combining industry-leading mass analyzer technology analysis with intelligent acquisition, intuitive software and remote, hands free calibration, discover a new level of improved confidence in structure elucidation, with on-the-fly identification of small molecules to reveal more chemical structures with ease.The Orbitrap IQ-X Tribrid MS seamlessly integrates with industry-leading solutions to provide a complete workflow solution, including Thermo Scientific™ Vanquish™ Duo UHPLC Systems, Thermo Scientific™ FAIMS Pro Duo interface for differential ion mobility, <a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/products/1759">Thermo Scientific™ Compound Discoverer™ data analysis software</a>, and the most comprehensive spectral libraries available on <a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/products/1764">Thermo Scientific™ mzCloud™</a>, enabling an intelligent MSⁿ data acquisition strategy to easily conduct small molecule structural elucidation, identification, and characterization.<img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Pragolab_Thermo_Orbitrap_IQ_X_Tribrid_hmotnostni_spektrometr_1_a565298840/Pragolab-Thermo-Orbitrap-IQ-X-Tribrid-hmotnostni-spektrometr-1.webp" alt="Thermo Scientific™ Orbitrap™ IQ-X™ Tribrid™ Mass Spectrometer" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Pragolab_Thermo_Orbitrap_IQ_X_Tribrid_hmotnostni_spektrometr_1_a565298840/thumbnail_Pragolab-Thermo-Orbitrap-IQ-X-Tribrid-hmotnostni-spektrometr-1.webp 238w," sizes="100vw" width="1447" height="949"><h5>Other benefits of this mass spectrometry system include:</h5><ul><li>A combination of the best of quadrupole, linear ion trap and Thermo Scientific™ Orbitrap™ mass analyzer technology for acquisition of the richest MSⁿ data per sample.</li><li>Spend more time on results, less time on instrument set-up with the new Auto-Ready ion source for remote, hands free calibration, combined with the Thermo Scientific™ EASY-IC™ (Internal Calibration) source to deliver superior mass accuracy in every scan, mass calibration can be fully automated, increasing productivity.</li><li>Real-Time Library Search provides higher confidence small molecule analysis using on-the-fly spectral matching for decision-based triggering of MSⁿ scan events that increase certainty.</li><li>Expanded <a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/products/1763">Thermo Scientific™ AcquireX workflow</a> capabilities provide easy and intelligent acquisitions to find more compounds by integrating independent experiments into an automated workflow for efficient and comprehensive sample and study characterization.</li><li>Flexible fragmentation choices for structural determination of lipids, metabolites, and other small molecules utilizing Collision induced dissociation (CID), Higher-energy collisional dissociation (HCD) and optional Ultraviolet Photodissociation (UVPD).</li><li>Improved unknown analysis and fine isotope detection with optional 1,000,000 (1M) FWHM resolution at m/z 200.</li></ul><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Pragolab_Thermo_Orbitrap_IQ_X_Tribrid_hmotnostni_spektrometr_2_3b50aefc74/Pragolab-Thermo-Orbitrap-IQ-X-Tribrid-hmotnostni-spektrometr-2.webp" alt="Thermo Scientific™ Orbitrap™ IQ-X™ Tribrid™ Mass Spectrometer" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Pragolab_Thermo_Orbitrap_IQ_X_Tribrid_hmotnostni_spektrometr_2_3b50aefc74/thumbnail_Pragolab-Thermo-Orbitrap-IQ-X-Tribrid-hmotnostni-spektrometr-2.webp 245w," sizes="100vw" width="1880" height="822"><h3>Thermo Scientific™ Orbitrap™ IQ-X™ Tribrid™ Mass Spectrometer Specifications</h3><ul><li>Dimensions (D x W x H): 30 x 50 x 28 in. (77 x 127 x 70 cm)</li><li>Dynamic Range: &gt;5000 within a single scan</li><li>Mass Accuracy:&nbsp;<ul><li>Orbitrap mass accuracy:<ul><li>&lt;3 ppm RMS using external calibration</li><li>&lt;1 ppm RMS using internal calibration</li></ul></li></ul></li><li>Polarity Switching: One Full Cycle in 1.1 sec</li><li>Scan Rates:&nbsp;<ul><li>OTMSⁿ: Up to 40 Hz</li><li>ITMSⁿ: Up to 45 Hz</li></ul></li><li>Weight (Metric): 318 kg (700 lb.)</li><li>Mass Range: Full MS Mass Range: 50 to 2000 m/z to MSⁿ Mass Range: 40 to 2000 m/z</li><li>Max. Resolution: Up to 500,000 at m/z 200</li><li>Resolution:&nbsp;<ul><li>7500 to 500,000 (FWHM) at m/z 200</li><li>1M Option: up to 1,000,000 (FWHM) at m/z 200</li></ul></li><li>Type: Orbitrap IQ-X Tribrid MS IC</li><li>Unit Size: Each</li></ul>

Thermo Scientific™ Orbitrap Eclipse™ Tribrid™ Mass Spectrometer

With enhanced performance and usability, the Orbitrap Eclipse Tribrid MS makes it easy to produce the high-quality data required to drive the right decisions, while expanding the breadth of your work and pushing your science beyond today’s discovery.

Introducing the Thermo Scientific™ Orbitrap Eclipse™ Tribrid™ mass spectrometer, our newest generation instrument designed with your most difficult analytical challenges in mind. With enhanced performance and usability, the Orbitrap Eclipse Tribrid mass spectrometer makes it easy to produce the high-quality data required to drive the right decisions, while expanding the breadth of your work and pushing your science beyond today’s discovery.Obtain high confidence insights for very complex molecules with the Thermo Scientific™ Orbitrap Eclipse™ Tribrid™ mass spectrometer. It combines industry-leading versatility and usability with the exceptional performance afforded by its built-in intelligence and revolutionary Thermo Scientific™ Tribrid™ architecture to deliver an instrument designed to address the most difficult analytical challenges.<img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Pragolab_Thermo_Orbitrap_Eclipse_Tribrid_hmotnostni_spektrometr_1_622422d68a/Pragolab-Thermo-Orbitrap-Eclipse-Tribrid-hmotnostni-spektrometr-1.webp" alt="Thermo Scientific™ Orbitrap Eclipse™ Tribrid™ Mass Spectrometer" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Pragolab_Thermo_Orbitrap_Eclipse_Tribrid_hmotnostni_spektrometr_1_622422d68a/thumbnail_Pragolab-Thermo-Orbitrap-Eclipse-Tribrid-hmotnostni-spektrometr-1.webp 245w," sizes="100vw" width="596" height="310"><h5>This newest Tribrid platform includes:</h5><ul><li>Advanced Ion Management Technology (AIM+) with the new QR5 segmented quadrupole mass filter</li><li>Real-Time Search</li><li>Enhanced Vacuum Technology</li><li>Optional Proton Transfer Charge Reduction (PTCR)</li><li>Optional High Mass Range MSⁿ (HMRⁿ) mode</li></ul><h5>Collectively, these features make this instrument uniquely suited for:</h5><ul><li>Accurate and high-throughput full-proteome quantitation</li><li>Characterization of complex mixtures of protein or small molecule-based pharmaceuticals</li><li>Deciphering higher-order protein structures</li></ul>The Orbitrap Eclipse Tribrid MS makes it easy to tackle these difficult analyses and collect the high-quality data required to drive the right decisions, pushing your science beyond today's discovery.With its built-in intelligence and versatility, the Orbitrap Eclipse Tribrid MS makes it easy to produce the high quality data required to drive the right decisions.<ul><li>Exceptional precursor selectivity and sensitivity with the next generation quadrupole mass filter</li><li>Unmatched depth and accuracy of TMT analysis with novel Real-Time Search</li><li>High Mass Range MSⁿ (HMRⁿ) option for comprehensive analysis of native protein complexes</li><li>Unique Proton Transfer Charge Reduction (PTCR) option for simplifying complex top-down spectra</li><li>Full experimental flexibility with revolutionary instrument control</li><li>A wide range of optional functionalities for unprecedented versatility</li><li>Common user interface with <a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/products/401">Thermo Scientific™ Orbitrap Exploris™ 480</a> and <a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/products?category=20&amp;manufacturerItems=3&amp;search=TSQ">TSQ™ triple quadrupole mass spectrometers</a></li></ul><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Pragolab_Thermo_Orbitrap_Eclipse_Tribrid_hmotnostni_spektrometr_2_53ee609c22/Pragolab-Thermo-Orbitrap-Eclipse-Tribrid-hmotnostni-spektrometr-2.webp" alt="Thermo Scientific™ Orbitrap Eclipse™ Tribrid™ Mass Spectrometer" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Pragolab_Thermo_Orbitrap_Eclipse_Tribrid_hmotnostni_spektrometr_2_53ee609c22/thumbnail_Pragolab-Thermo-Orbitrap-Eclipse-Tribrid-hmotnostni-spektrometr-2.webp 245w," sizes="100vw" width="1452" height="710"><h3>Thermo Scientific™ Orbitrap Eclipse™ Tribrid™ Mass Spectrometer Specifications</h3><ul><li>Dimensions (D x W x H): 30 x 50 x 28 in. (77 x 127 x 70 cm)</li><li>Dynamic Range: &gt;5000 with a single OTMS spectrum</li><li>Mass Accuracy:&nbsp;<ul><li>Orbitrap mass accuracy:<ul><li>&lt;3 ppm RMS using external calibration</li><li>&lt;1 ppm RMS using internal calibration</li></ul></li></ul></li><li>Model: Tribrid MS</li><li>Polarity Switching: One Full Cycle in &lt; 1.1 sec</li><li>Scan Rates:&nbsp;<ul><li>OTMSⁿ: Up to 40 Hz</li><li>ITMSⁿ: Up to 45 Hz</li></ul></li><li>Weight (Metric): 180 kg (400 lb.)</li><li>Mass Range:&nbsp;<ul><li>Standard mass range: 50 to 2000 m/z</li><li>Mid mass range: 200 to 6000 m/z</li><li>Optomenal HMRⁿ mass range: 500 to 8000 m/z</li></ul></li><li>Max. Resolution: Up to 500,000 at m/z 200</li><li>Type: Orbitrap LC-MS</li><li>Unit Size: Each</li></ul>

Thermo Scientific Vanquish Duo UHPLC Systems

A Vanquish Duo UHPLC system enables you to run two methods simultaneously on one instrument, doubling your throughput or deepening your sample knowledge while maximizing your productivity and performance.

<h3>Optimize your productivity</h3>In today’s challenging research and production environments, you are being asked to be more productive without increasing spending or compromising quality.&nbsp;At the same time, advancements and innovations are needed to improve operational efficiency and performance. How do you accomplish this? It’s easy with the Thermo Scientific™ Vanquish™ High-throughput LC systems built for enhanced productivity. Vanquish High-throughput LC systems combine individual Vanquish components and software wizards to deliver increased throughput or detector utilization.<img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Thermo_Scientific_Vanquish_Duo_UHPLC_Systems_8_2befa2ab8e.jpg" alt="Thermo Scientific Vanquish Duo UHPLC Systems." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Thermo_Scientific_Vanquish_Duo_UHPLC_Systems_8_2befa2ab8e.jpg 191w," sizes="100vw" width="1325" height="1086"><h3>Two flow paths for maximized productivity</h3>Your laboratory may be facing increased pressure to improve working efficiency and deal with limiting resources, such as budget, space, or manpower. Vanquish High-throughput LC systems are designed to overcome these limitations by providing multiple flow paths for parallelization of specific stages of the LC run.<img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Thermo_Scientific_Vanquish_Duo_UHPLC_Systems_2_06370d93f8.jpg" alt="Thermo Scientific Vanquish Duo UHPLC Systems." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Thermo_Scientific_Vanquish_Duo_UHPLC_Systems_2_06370d93f8.jpg 245w," sizes="100vw" width="955" height="591"><h4>Unique dual technology</h4>The Vanquish platform contains two unique dual modules. Vanquish dual pumps merge two individual, low pressure mixing pumps in one housing enabling separate eluents and gradients for each pump.Vanquish dual split samplers possess two independently controlled injection units including separate injection valves and injection needles. Used in Dual LC and LC-MS workflows only, this enables high throughput and extensive sample characterization workflows.<h3>Two configurations to best serve your needs</h3>Vanquish High-throughput workflows are configured with Thermo Scientific™ Vanquish™ Core system, Thermo Scientific™ Vanquish™ Flex system or Thermo Scientific™ Vanquish™ Horizon system modules. Both workflows make use of two pumps to increase instrument productivity.<img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Thermo_Scientific_Vanquish_Duo_UHPLC_Systems_3_77acff4bea.jpg" alt="Thermo Scientific Vanquish Duo UHPLC Systems: Two configurations to best serve your needs." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Thermo_Scientific_Vanquish_Duo_UHPLC_Systems_3_77acff4bea.jpg 245w," sizes="100vw" width="1682" height="735"><h4>Vanquish Duo UHPLC Systems</h4><ul><li>Supported systems:<ul><li><a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/products/744">Vanquish Horizon</a></li><li><a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/products/743">Vanquish Flex</a></li></ul></li><li>Sampler: Dual split sampler</li><li>Detector: Two out of the complete detector portfolio</li><li>Software control:<ul><li><a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/products/76">Thermo Scientific™ Chromeleon™ Chromatography Data System (CDS)</a></li><li><a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/products/1838">Thermo Scientific™ Standard Instrument Integration (SII)</a> for <a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/products/627">Waters™ Empower™ 3 CDS</a></li></ul></li></ul><h4>Vanquish Tandem LC Systems</h4><ul><li>Supported systems:<ul><li><a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/products/744">Vanquish Horizon</a></li><li><a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/products/743">Vanquish Flex</a></li><li><a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/products/397">Vanquish Core</a></li></ul></li><li>Sampler: Split sampler</li><li>Detector: One out of the complete detector portfolio and/or one mass spectrometer</li><li>Software control:<ul><li><a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/products/76">Chromeleon CDS</a></li><li>Standard System Integration for <a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/products/1758">Thermo Scientific™ Xcalibur™ software</a></li></ul></li></ul><h3>Vanquish Duo UHPLC systems</h3>A Vanquish Duo UHPLC system enables you to run two methods simultaneously on one instrument, doubling your throughput or deepening your sample knowledge while maximizing your productivity and performance. The unique Vanquish Duo UHPLC system will:<ul><li>Improve return on investment by reducing cost per sample</li><li>Increase sample throughput without changing validated methods</li><li>Increase capacity without sacrificing bench space</li><li>Accelerate your method development process</li><li>Analyze the same set of samples with multiple methods, reducing sample preparation time and maximizing sample knowledge</li></ul><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Thermo_Scientific_Vanquish_Duo_UHPLC_Systems_4_4cb62100fd.jpg" alt="Thermo Scientific Vanquish Duo UHPLC Systems." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Thermo_Scientific_Vanquish_Duo_UHPLC_Systems_4_4cb62100fd.jpg 245w," sizes="100vw" width="1678" height="512"><h4>What can you do with a Vanquish Duo UHPLC system?</h4><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Thermo_Scientific_Vanquish_Duo_UHPLC_Systems_5_84912c40d4.jpg" alt="Thermo Scientific Vanquish Duo UHPLC Systems: What can you do with a Vanquish Duo UHPLC system?" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Thermo_Scientific_Vanquish_Duo_UHPLC_Systems_5_84912c40d4.jpg 245w," sizes="100vw" width="1604" height="658"><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Thermo_Scientific_Vanquish_Duo_UHPLC_Systems_6_f8939b888a.jpg" alt="Thermo Scientific Vanquish Duo UHPLC Systems: Two independent assays run simultaneously from a single sample on one LC instrument to deepen your sample knowledge." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Thermo_Scientific_Vanquish_Duo_UHPLC_Systems_6_f8939b888a.jpg 245w," sizes="100vw" width="1372" height="385"><h4>Complete control with just a few clicks</h4><a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/products/76">Chromeleon CDS</a> delivers industry-leading ease-of-use for the Vanquish Duo system. Independently control the two channels of the Vanquish Duo system from a single interface with full traceability of all actions that is 21 CFR Part 11 compliant.<img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Thermo_Scientific_Vanquish_Duo_UHPLC_Systems_c2d9ed2c2c.jpg" alt="Thermo Scientific Vanquish Duo UHPLC Systems: Easy-to-use and intuitive system control of the Dual LC workflow within Chromeleon CDS." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Thermo_Scientific_Vanquish_Duo_UHPLC_Systems_c2d9ed2c2c.jpg 245w," sizes="100vw" width="1190" height="632"><h3>See the Vanquish Duo UHPLC system</h3>The Vanquish Duo UHPLC system has been built to utilize a wide range of analytical detectors, including mass spectrometry (MS), diode array detection (DAD), charged aerosol detection (CAD), variable wavelength detection (VWD), and fluorescence detection (FLD).<img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Thermo_Scientific_Vanquish_Duo_UHPLC_Systems_7_c288dc2658.jpg" alt="Thermo Scientific Vanquish Duo UHPLC Systems: See the Vanquish Duo UHPLC system." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Thermo_Scientific_Vanquish_Duo_UHPLC_Systems_7_c288dc2658.jpg 174w," sizes="100vw" width="939" height="841"><h5>1. Vanquish Solvent Monitor</h5>Use the Vanquish Solvent Monitor to increase your regulatory compliance, productivity, and safety of your laboratory. That device guarantees that your HPLC systems will never run dry and the waste will not spill over during your analysis, reducing loss of valuable measurement time and sample material.<h5>2. Vanquish User Interface</h5>With the Vanquish User Interface, you are the master of your LC instrument. The display always shows you the key parameters of your instrument such as flow and pressure and guides you in a visual step-by-step video tutorial through key maintenance tasks.<h5>3. Versatile detection capabilities</h5>Differing molecular characteristics of your analyte, from small molecules to complex biomolecules, necessitate multiple detector methodologies. Vanquish Duo UHPLC systems can be used with any two detectors out of the broad detector portfolio including MS, DAD, VWD, FLD and CAD.<h5>4. Compatible with your software infrastructure</h5>A Vanquish Duo UHPLC system can be operated in various software environments including the latest Chromeleon software feature release, Chromeleon CDS long-term, and extended support version and Waters Empower.<h5>5. Versatile column management</h5>Greater column capacity (two 30 cm columns or a greater number of shorter columns per compartment), plus class-leading temperature range and temperature stability.<h5>6. Delivering powerful separations</h5>Run samples with two similar or different columns to increase throughput or maximize output. Setup with optional second compartment for independent column temperatures.<h5>7. Easier operation</h5>Patented tool-free Thermo Scientific™ Viper™ Fingertight Fittings with near-zero dead volume operation and ergonomic design.<h5>8. Unique Thermo Scientific™ SmartInject technology</h5>Superior retention time precision and enhanced column protection with intelligent SmartInject technology on both flow paths.<h5>9. Two proprietary injection valves</h5>Two separate injection valves with no shared fluidics allowing full application flexibility, including individual eluents, wash liquids and loop sizes.<h5>10. Easier sample handling</h5>Automated barcode reader to eliminate tedious rack configuration.<h5>11. Higher sample capacity</h5>High sample capacity is standard (four plates versus two plates on industry average). Increase capacity further with the optional Thermo Scientific™ Vanquish™ Charger module.<h5>12. Accurate flow for more data confidence</h5>The flow is delivered by a dual pump offering two truly individual devices in a single housing with ternary gradient proportioning.<h5>13. Simple maintenance</h5>Module maintenance is reduced to a minimum and can be worked on without instrument de-stacking, plus smart light bars on the front of the system provide instant system status update.<h5>14. Keep the lab tidy</h5>The built-in drawer stores accessories, related documents, or any other useful material for the system.<h3>Thermo Scientific LC portfolio overview</h3><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Thermo_Scientific_LC_portfolio_overview_2db5044366.jpg" alt="Thermo Scientific LC portfolio overview." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Thermo_Scientific_LC_portfolio_overview_2db5044366.jpg 188w," sizes="100vw" width="795" height="660">

Thermo Scientific Vanquish FLEX UHPLC System

Whether you are analyzing innovative biotherapeutics or novel chemical medicines, method development with Thermo Scientific™ Vanquish™ Flex™ UHPLC system has never been easier.

Thermo Scientific Vanquish Horizon UHPLC System

Designed with innovative technology and fine detail, the Vanquish Horizon UHPLC delivers outstanding precision, sensitivity, and simplicity for unmatched performance and robustness.

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