<p><strong>A nitrogen-enriched graphene nanomaterial (GN3) for energy storage in supercapacitors, developed by researchers at the Czech Advanced Technology and Research Institute (CATRIN) of Palacký University Olomouc, has been included in the European Commission’s </strong><a target="_blank" rel="noopener noreferrer" href="https://innovation-radar.ec.europa.eu/innovation/66621"><strong>Innovation Radar database as a technology with strong commercial potential</strong></a><strong>. The material has been assessed as highly market-ready, with significant potential to create new markets. Palacký University, which coordinated the prestigious European Innovation Council project TRANS2DCHEM, has also been recognised among the project’s key innovators.</strong></p><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/CATRIN_Grafenovy_material_z_Olomouce_se_dostal_mezi_perspektivni_evropske_inovace_0d2a178219.jpg" alt="CATRIN: Graphene nanomaterial developed in Olomouc recognised among Europe’s most promising innovations" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_CATRIN_Grafenovy_material_z_Olomouce_se_dostal_mezi_perspektivni_evropske_inovace_0d2a178219.jpg 156w," sizes="100vw" width="480" height="480"></p><p>Innovation Radar is a European platform that identifies promising innovations and key innovators emerging from research and innovation projects funded by the European Union.</p><p>“This is a great honour and recognition of our work. The excellent assessment within the Innovation Radar platform demonstrates the potential of CATRIN—and of Palacký University as a whole—to transform scientific discoveries into products with strong commercial potential. Recognition by independent experts highlights the technology’s high level of market readiness, which is exceptional even in the context of other outstanding projects supported by the European Innovation Council,” said <strong>Jiří Navrátil, Technology Transfer Coordinator at CATRIN</strong>.</p><p>The nitrogen-enriched graphene electrode material, based on graphene—a two-dimensional material consisting of a single layer of carbon atoms—was developed by researchers in Olomouc seven years ago. It originated from <strong>Michal Otyepka’s</strong> prestigious European Research Council project focused on the <strong>2D chemistry of fluorographene</strong>.</p><p>In the following years, researchers demonstrated the material’s significant potential for electrical energy storage, particularly in supercapacitors, which represent a promising alternative to today’s widely used lithium-ion batteries. Development of prototype supercapacitors with unique properties began under the prestigious European Innovation Council project <strong>TRANS2DCHEM</strong>, in collaboration with partners from<strong> Bar-Ilan University in Israel</strong> and the<strong> Italian company ITELCOND</strong>. The next logical step was the establishment of the spin-off company <strong>Atomiver</strong>, which has been operating since 2024.</p><p>“The continuous growth in global energy consumption and the rapid expansion of electronic devices are driving strong demand for high-performance, stable energy storage components. Our electrode material is unique because it pushes the current limits of commercially available technologies, particularly in terms of volumetric energy density—the amount of electrical energy that can be stored within a given volume. Compared with existing technologies, our material enables several-fold higher energy density. Its inclusion in the Innovation Radar database encourages us to continue developing the technology and bring it closer to commercial production,” said <strong>Michal Otyepka</strong>, one of the inventors of the technology and co-owner of the spin-out company.</p><p>The material is protected by <strong>several international patents</strong>, which have been licensed by Atomiver from Palacký University.</p><p>“Supercapacitors incorporating our graphene material could one day help ensure the safe operation of large data centres, Internet of Things (IoT) networks, transport systems, grid-scale energy storage, electronics for space applications and implantable medical devices,” added<strong> Veronika Šedajová</strong>, one of the inventors of the technology.</p><p>The significance of the technology was previously recognised when Atomiver was selected for the<strong> NATO DIANA </strong>accelerator programme, one of only<strong> 74 companies chosen from more than 2,600 applicants</strong>. The company has also received support from CzechInvest through its Technology Incubation programme.</p><p>“We are currently in intensive discussions with investors, who have shown strong interest in the technology, not least because the electrode material we have developed also has considerable potential for applications in other industries,” said<strong> Andrew Hladký, Director of Atomiver</strong>.</p><p><a target="_blank" rel="noopener noreferrer" href="https://innovation-radar.ec.europa.eu/innovation/66621"><strong>Innovation Radar</strong></a> serves as a platform for investors, entrepreneurs and the wider public to discover emerging technologies.</p>

Graphene nanomaterial developed in Olomouc recognised among Europe’s most promising innovations

<h3><strong>HPLC Detection Technologies: A Practical Overview</strong></h3><p><strong>High-performance liquid chromatography is a cornerstone of modern analytical chemistry. It is routinely applied in pharmaceutical quality control, biopharmaceutical development, environmental monitoring, food analysis, and polymer characterization. However, separating the components of a sample is only one part of the analytical process. The compounds leaving the column must also be recognized and converted into a signal that can be evaluated.</strong></p><p>This is the task of the HPLC detector. Its selection can strongly influence the sensitivity, selectivity, reliability, and overall usefulness of a chromatographic method.</p><p>In the first article of our HPLC detector series, we introduce the principal detection technologies, explain how they differ, and outline the factors that should be considered when selecting a detector for a particular application.</p><h4><strong>What Does an HPLC Detector Do?</strong></h4><p>After compounds have been separated inside the chromatographic column, they enter the detector one after another. The detector continuously monitors the column effluent and responds to a physical or chemical property of each analyte.</p><p>This response is converted into an electrical signal and displayed as a chromatogram. The position of a peak provides information related to retention time, while its height or area can be used to determine the amount of the corresponding compound.</p><p>A detector must therefore provide more than a visible signal. It should generate data that are sufficiently sensitive, stable, reproducible, and suitable for the purpose of the analytical method.</p><p>Several characteristics are particularly important when comparing detector technologies:</p><ul><li><strong>Sensitivity:</strong> The lowest amount or concentration that can be detected or reliably quantified.</li><li><strong>Selectivity:</strong> The ability to respond preferentially to certain compounds while minimizing signals from other sample components.</li><li><strong>Linearity and working range:</strong> The concentration interval over which detector response remains proportional to analyte concentration.</li><li><strong>Mobile-phase compatibility:</strong> The detector must function correctly with the selected solvents, additives, gradients, flow rates, and temperatures.</li><li><strong>Information content:</strong> Some detectors provide only a quantitative response, while others also deliver spectral or structural information.</li><li><strong>Practical requirements:</strong> Instrument cost, maintenance, method complexity, and operator experience must also be considered.</li></ul><p>There is consequently no single detector that is ideal for every separation. The most suitable choice depends on the analyte, sample matrix, expected concentration, mobile phase, and type of information required.</p><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/KNAUER_Figure_1_Typical_HPLC_system_configuration_with_two_detectors_d909b878b2.webp" alt="KNAUER: Figure 1 - Typical HPLC system configuration with two detectors." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_KNAUER_Figure_1_Typical_HPLC_system_configuration_with_two_detectors_d909b878b2.webp 245w," sizes="100vw" width="1024" height="531"></p><h4><strong>How HPLC Detector Responses Can Be Classified</strong></h4><p>HPLC detection principles can be viewed from three broad perspectives.</p><h5><strong>Analyte-property detection</strong></h5><p>These detectors respond to a characteristic of the individual compound, such as UV absorption, fluorescence, optical activity, or electrochemical behavior. Their response may therefore be highly selective, but only analytes possessing the relevant property can be measured.</p><h5><strong>Bulk-property detection</strong></h5><p>Bulk-property detectors monitor a change in a general physical property of the mobile phase as an analyte passes through the detector. Refractive index is a common example. Because many compounds can produce such a change, these detectors offer broad applicability, although they are often less sensitive and more dependent on stable operating conditions.</p><h5><strong>Hyphenated detection techniques</strong></h5><p>In hyphenated systems, HPLC is connected to another analytical technology capable of generating additional information. Liquid chromatography–mass spectrometry is the best-known example. These combinations extend detection beyond a simple chromatographic response and can provide molecular-mass or structural data.</p><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/KNAUER_Table_1_Common_HPLC_Detectors_8b54ef057f.webp" alt="KNAUER: Table 1 - Common HPLC Detectors." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_KNAUER_Table_1_Common_HPLC_Detectors_8b54ef057f.webp 245w," sizes="100vw" width="1024" height="228"></p><h4><strong>1. Optical Detectors</strong></h4><p>Optical detection is the most established and widely used approach in routine liquid chromatography. These detectors evaluate how an analyte absorbs, emits, rotates, or otherwise interacts with light.</p><h5><a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/products?category=175&amp;manufacturerItems=503"><strong>UV/VIS</strong></a><strong>, </strong><a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/products?category=177&amp;manufacturerItems=503"><strong>DAD, and PDA detectors</strong></a></h5><p>UV/VIS absorbance detectors measure the reduction in light intensity caused by an analyte at a selected wavelength. They are especially suitable for compounds containing chromophores, which are molecular structures capable of absorbing ultraviolet or visible light.</p><p>Diode-array and photodiode-array detectors record absorbance across a broader wavelength range. In addition to quantitative detection, they can provide spectral information that assists with wavelength selection, peak comparison, and the assessment of chromatographic purity.</p><p>UV-based detectors are popular because they are robust, relatively easy to operate, and compatible with a wide range of routine HPLC applications.</p><h5><a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/products?category=297&amp;manufacturerItems=503"><strong>Fluorescence detectors</strong></a></h5><p>Fluorescence detection measures light emitted by a compound after excitation at a specific wavelength. It can provide excellent sensitivity and selectivity when an analyte is naturally fluorescent.</p><p>Compounds without native fluorescence may sometimes be converted into fluorescent derivatives before or after separation. This extends the range of applications but introduces an additional sample-preparation or reaction step.</p><p>Fluorescence detection is frequently used for pharmaceutical compounds, environmental contaminants, biological molecules, and other analytes requiring sensitive measurement in complex samples.</p><h5><strong>Optical rotation and circular dichroism detectors</strong></h5><p>These detectors evaluate the interaction of optically active compounds with polarized light. They are particularly valuable in chiral separations, where the objective is to distinguish between enantiomers and evaluate enantiomeric purity.</p><p>Their application is more specialized than conventional UV detection, but they can provide information that cannot be obtained from a standard absorbance signal alone.</p><h4><strong>2. Aerosol and Light-Scattering Detectors</strong></h4><p>Some analytes do not absorb UV light strongly enough for reliable absorbance detection. Aerosol-based and light-scattering technologies provide alternatives for many nonvolatile compounds.</p><h5><strong>Evaporative light-scattering detection</strong></h5><p>In an evaporative light-scattering detector, the column effluent is nebulized and the volatile mobile phase is removed. The remaining analyte particles pass through a light beam, and the scattered light is measured.</p><p>ELSD can therefore detect many compounds that lack chromophores, including certain lipids, carbohydrates, surfactants, and polymers. The analyte must generally be less volatile than the mobile phase.</p><h5><strong>Charged aerosol detection</strong></h5><p>Charged aerosol detection also begins with nebulization and evaporation of the mobile phase. The resulting analyte particles are then electrically charged, and the charge is measured to generate the detector response.</p><p>CAD offers broad applicability for nonvolatile and semi-volatile analytes and is frequently considered when UV detection is unavailable or produces an insufficient response.</p><h5><strong>Multi-angle light scattering</strong></h5><p>Multi-angle light-scattering detectors measure light scattered at several angles. When combined with suitable chromatographic and concentration data, MALS can support the characterization of molecular size and absolute molar mass.</p><p>This capability is particularly valuable in polymer analysis, protein characterization, and size-exclusion chromatography.</p><h4><strong>3. Mass Spectrometric Detection</strong></h4><p>Mass spectrometry is among the most informative detection technologies available for liquid chromatography. Instead of responding only to absorbance or another general property, an MS detector measures ions according to their mass-to-charge ratio.</p><p>LC–MS can provide:</p><ul><li>High sensitivity for trace-level analysis</li><li>Strong selectivity in complex sample matrices</li><li>Molecular-mass information</li><li>Fragmentation data supporting compound identification</li><li>Qualitative and quantitative results within a single workflow</li></ul><p>Mass spectrometry is not universal in the strictest sense because the analyte must be successfully ionized. The mobile phase and its additives must also be compatible with the ion source.</p><p>Despite these requirements, LC–MS has become indispensable in pharmaceutical research, clinical and bioanalytical testing, food safety, environmental analysis, metabolomics, and many other fields. Its main disadvantages are higher acquisition costs, greater method complexity, and more demanding maintenance and data interpretation.</p><h4><strong>4. Electrochemical Detectors</strong></h4><p>Electrochemical detectors measure electrical changes associated with oxidation or reduction reactions at an electrode surface. They are suitable for compounds that are electrochemically active under the selected conditions.</p><p>Typical applications include the analysis of:</p><ul><li>Neurotransmitters</li><li>Catecholamines</li><li>Vitamins</li><li>Phenolic compounds</li><li>Redox-active pharmaceutical substances</li></ul><p>For suitable analytes, electrochemical detection can achieve very high sensitivity and selectivity. At the same time, reliable operation requires carefully controlled mobile-phase composition, clean solvents, stable electrode conditions, and thorough method optimization.</p><p>The technique is therefore extremely powerful for targeted applications but less broadly applicable than UV or mass spectrometric detection.</p><h4><strong>5. Bulk-Property and Specialized Detectors</strong></h4><h5><a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/products/2429"><strong>Refractive index detectors</strong></a></h5><p>A refractive index detector compares the refractive index of the mobile phase with that of the mobile phase containing an eluting analyte. Because many compounds alter this property, RID can be used for substances that do not absorb UV light.</p><p>Common applications include sugars, alcohols, lipids, and polymers. Refractive index detection is relatively straightforward, but it generally provides lower sensitivity than UV or fluorescence detection. It is also highly sensitive to changes in temperature and mobile-phase composition, making it poorly suited to gradient elution.</p><h5><strong>Viscosity and pH detectors</strong></h5><p>Viscosity detectors monitor changes in the flow characteristics of the mobile phase, while pH detectors respond to changes in acidity or alkalinity. These technologies are less common in general-purpose HPLC but can be useful in specific analytical or characterization workflows.</p><h5><strong>Radioactivity detectors</strong></h5><p>Radioactivity detectors are used when compounds contain radioactive labels. They are particularly important in tracer studies, metabolism research, drug development, and nuclear medicine.</p><p>Their selectivity is determined by the radioactive signal, allowing labelled compounds and their products to be followed even in complex matrices.</p><h4><strong>Selecting the Most Appropriate Detector</strong></h4><p>The detector should be selected according to the analytical question rather than according to a single performance specification.</p><p>For routine analysis of compounds with suitable chromophores, UV or diode-array detection is often the most practical solution. Fluorescence or electrochemical detection may be preferable when very high sensitivity is required for compounds with the appropriate chemical properties.</p><p>ELSD and CAD are useful for nonvolatile analytes that exhibit weak or no UV absorption. Refractive index detection remains valuable for relatively concentrated samples analyzed under isocratic conditions. When molecular identification, structural confirmation, or highly selective trace analysis is required, mass spectrometry is usually the strongest option.</p><p>In more complex workflows, several detectors may be connected in sequence. This makes it possible to obtain complementary information from the same chromatographic separation.</p><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/KNAUER_Figure_2_Detector_types_used_in_HPLC_b64aecb606.webp" alt="KNAUER: Figure 2 Detector types used in HPLC." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_KNAUER_Figure_2_Detector_types_used_in_HPLC_b64aecb606.webp 245w," sizes="100vw" width="1024" height="346"></p><h4><strong>Conclusion</strong></h4><p>A successful chromatographic separation must be paired with a detector capable of revealing the compounds of interest. Detector selection directly affects what can be measured, at what concentration, and with what degree of confidence.</p><p>Understanding the underlying detection principles makes it easier to balance sensitivity, selectivity, compatibility, information content, and practical laboratory requirements. Rather than searching for a universally superior technology, analysts should choose the detector whose response best matches the properties of the analyte and the objective of the method.</p><p>The next article in this series will examine detector performance in greater detail, including the specifications and evaluation criteria that make it possible to compare different technologies more fairly.</p>

Seeing is Believing – Detection Made Easy Part 1: Detector Overview

<p><strong>Our Library never stops expanding. What are the most recent contributions to LabRulezLCMS Library in the week of 10th August 2026? Check out new documents from the field of liquid phase, especially HPLC and LC/MS techniques!</strong></p><blockquote><p><span style="font-size:20px;">👉 </span><a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/library"><span style="font-size:20px;"><strong>SEARCH THE LARGEST REPOSITORY OF DOCUMENTS ABOUT LCMS AND RELATED TECHNIQUES</strong></span></a></p></blockquote><blockquote><p><strong>👉 Need info about different analytical techniques?</strong> Peek into <a target="_blank" rel="noopener noreferrer" href="https://gcms.labrulez.com/library"><strong>LabRulezGCMS</strong></a> or <a target="_blank" rel="noopener noreferrer" href="https://icpms.labrulez.com/library"><strong>LabRulezICPMS</strong></a> libraries.</p></blockquote><p>This week we bring you posters by <strong>Agilent Technologies / ASMS, Thermo Fisher Scientific / ASMS </strong>and<strong> Waters Corporation / ASMS </strong>and application notes by<strong> Metrohm and Shimadzu!</strong></p><h3><strong>1. Agilent Technologies / ASMS: Impurity Monitoring of GLP-1 Therapeutic Peptide RetatrutideUsing a 2D-LC/TOF System</strong></h3><ul><li>Poster</li><li><a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/labrulez-bucket-strapi-h3hsga3/glp_1_impurity_monitoring_2d_lc_tof_asms_2026_mp_388_agilent_eadce3f244.pdf"><strong>Full PDF for download</strong></a></li></ul><p>Retatrutide is a next-generation triple agonist (GLP1/GIP/glucagon) and an emerging candidate among multivalent GLP1–based therapeutics. Its structural complexity and diverse impurity profile challenge conventional one-dimensional LC methods, which often lack sufficient resolving power to separate closely related impurities, sequence variants, and synthetic byproducts.&nbsp;</p><p>In this study, we demonstrate an Agilent multiple heart-cutting 2D-LC platform that enables rapid online solvent exchange and enhanced chromatographic selectivity, allowing targeted isolation of impurity peaks from a TFA-based first-dimension separation. When coupled with the Agilent 6230C Accurate Mass TOF LC/MS system, the workflow delivers fast, sensitive, and accurate mass profiling under MS-friendly second-dimension conditions, providing a robust solution for comprehensive impurity characterization of GLP-1 peptides such as Retatrutide.</p><h4><strong>Experimental</strong></h4><h5><strong>Instrumentation&nbsp;</strong></h5><ul><li><a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/library?instrumentationItems=39&amp;mainauthorItems=10&amp;search=1290+infinity+III+2D-LC"><strong>Agilent 1290 Infinity III 2D-LC system</strong></a><strong> comprised the following modules:&nbsp;</strong><ul><li>1290 Infinity III Bio Multisampler with Thermostat (G7137A)&nbsp;</li><li>1290 Infinity III Multicolumn Thermostat (G7116B)&nbsp;</li><li>1290 Infinity III Diode Array Detector (G7117B) with Max-Light cartridge cell (G7117-60020)&nbsp;</li><li>1260 Infinity III Bio Flexible Pump (G7131C)&nbsp;</li><li>1290 Infinity III Bio High-speed Pump (G7132A)&nbsp;</li><li>1290 Infinity Valve Drive (G1170A) with 2D-LC ASM Valve (G5643B)&nbsp;</li><li>Two 1290 Infinity III Valve Drives (G1170A) with multiple heart-cutting valves (G5642-64000) equipped with 40 µL Bio loops&nbsp;</li></ul></li><li><a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/library?mainauthorItems=10&amp;search=6230C"><strong>Agilent 6230C Accurate Mass TOF LC/MS System</strong></a></li></ul><h4><strong>Conclusions</strong></h4><p>Agilent 2D-LC with multiple heart-cutting effectively bridges TFA-driven chromatographic performance with FA-compatible MS detection, overcoming ion suppression limitations.&nbsp;</p><p>The integrated 2D-LC/Accurate Mass TOF workflow delivers confident impurity identification and robust characterization, supporting peptide analysis in both QA/QC and development environments.</p><h3><strong>2. Metrohm: Vinylene carbonate water determination via coulometric Karl Fischer titration</strong></h3><p><strong>Water determination in vinylene carbonate by Karl Fischer coulometric titration made simple with alcohol-free reagents</strong></p><ul><li>Application note</li><li><a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/labrulez-bucket-strapi-h3hsga3/an_k_076_download_8658665940.pdf"><strong>Full PDF for download</strong></a></li></ul><p>Vinylene carbonate (vc) is commonly used as a battery electrolyte additive in lithium-ion batteries. Its popularity is due to the positive impact it has on anode cyclability and reduction of irreversible capacity. Small amounts of moisture in Li-ion batteries can significantly decrease performance and lifetime. Therefore, moisture levels in electrolyte solutions and their additives are controlled for use in manufacturing. Until recent years, accurate moisture quantification in vinylene carbonate samples was difficult due to side reactions. With the commercial development of alcohol-free coulometric Karl Fischer reagents, accurate determination is now much more accessible to end-users.</p><h4><strong>EXPERIMENTAL</strong></h4><p>This analysis was performed using an <a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/library?mainauthorItems=220&amp;search=852+titrando"><strong>852 Titrando</strong></a> (2.852.0060) instrument, equipped with the electrodes listed in Table 1.</p><h4><strong>CONCLUSION&nbsp;</strong></h4><p>During battery manufacturing, control of all moisture present is important to guarantee adequate performance in the finished product. More easily quantifying the moisture contribution from additives such as vinylene carbonate makes this a much simpler task. The introduction of alcohol-free Karl Fischer reagents accomplishes this effectively, allowing the generation of accurate and repeatable moisture data—even for a difficult sample matrix.</p><h3><strong>3. Shimadzu: Non-Targeted Analysis of Metabolites in Alcoholic Beverages Using LabSolutions Insight Profiler</strong></h3><ul><li>Application note</li><li><a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/labrulez-bucket-strapi-h3hsga3/an_01_01241_en_57b43edb57.pdf"><strong>Full PDF for download</strong></a></li></ul><h5><strong>User Benefits</strong></h5><ul><li>Using an LC-QTOFMS system with LabSolutions Insight Profiler non-targeted analysis software enables comprehensive comparative analysis of metabolites in food samples.&nbsp;</li><li>Principal component analysis and volcano plots can be used to detect characteristic features in each sample.&nbsp;</li><li>A variety of functions, including MS/MS spectral library searching, enable easy qualitative analysis of components.</li></ul><p>Metabolomics, a technique that analyzes metabolites in living organisms, is increasingly being applied in the food industry for product development and quality control. A particularly powerful approach is non-targeted metabolomics, which aims to analyze not only known metabolites but also unknown compounds without prior assumptions to reveal unexpected chemical differences between samples. Quadrupole time-of-flight mass spectrometry (QTOFMS), with its accurate-mass capability, is well suited for this purpose. However, the large volume of generated data creates challenges for feature identification and data comparison.&nbsp;</p><p>This article presents a case study in which LabSolutions Insight Profiler, a software platform for non-targeted analysis, was used to characterize compounds in beer and beer-like beverages. Peak detection, retention-time alignment, and library searching were performed across multiple datasets. Multivariate analysisrevealed compound profiles that likely reflect the differences in raw materials and manufacturing processes, demonstrating the utility of the approach for beverage characterization.</p><h4><strong>LabSolutions Insight Profiler&nbsp;</strong></h4><p><a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/library?mainauthorItems=1&amp;search=LabSolutions+Insight+Profiler"><strong>LabSolutions Insight Profiler </strong></a>is software for batch processing of LC-QTOFMS data that enables non-targeted analysis through an intuitive workflow. It readily supports easy spectral feature detection, alignment, statistical processing, filtering, list screening, MS/MS spectra-based library searching, principal component analysis (PCA), displaying volcano or box plots, and more. Fig. 1 shows the data analysis workflow.</p><h4><strong>Analysis Conditions&nbsp;</strong></h4><p><a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/library?mainauthorItems=1&amp;search=nexera+X3"><strong>Nexera X3 UHPLC</strong></a> and<strong> </strong><a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/library?mainauthorItems=1&amp;search=9030"><strong>LCMS-9030 systems</strong></a> were used as the analytical instruments. The LC method included in the LC/MS/MS Method Package for Primary Metabolites was used as the method. The data-dependent acquisition (DDA) mode was used to simultaneously acquire precursor m/z and MS/MS data. Table 2 shows the analysis conditions.</p><h4><strong>Conclusion&nbsp;</strong></h4><p>In this study, compounds in various beer and beer-like beverages were analyzed by non-targeted analysis using an LCMS-9030 quadrupole time-of-flight mass spectrometer system. Analyzing compounds characteristic of each sample with LabSolutions Insight Profiler software confirmed that the resulting profiles reflected differences in raw materials and manufacturing methods.&nbsp;</p><p>LabSolutions Insight Profiler enables efficient non-targeted analysis by processing LC-QTOFMS data with an intuitive workflow. This workflow allows for objective evaluation of the taste, quality, and nutritional value of food products. Furthermore, this method is applicable not only to the analysis of compounds in beverages but also to non-targeted analysis of other types of samples.</p><h3><strong>4. Thermo Fisher Scientific / ASMS: High throughput targeted proteomic workflows with Adaptive Retention Time on the </strong><a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/products/1959"><strong>Orbitrap Astral Zoom MS</strong></a></h3><ul><li>Poster</li><li><a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/labrulez-bucket-strapi-h3hsga3/po_004699_lsms_art_astral_zoom_proteomics_po004699_asms2026_na_en_78767efdc9.pdf"><strong>Full PDF for download</strong></a></li></ul><p>Advances in MS instrumentation have accelerated the identification of biomarkers, but validating and translating these discoveries into assays for clinical use remains challenging and typically requires robust and accurate targeted approaches. While these often rely on predetermined reference target retention time (RT) windows, analytical column degradation or replacement, aging solvents, sample matrix effects, etc. can cause peptide RT shifts between injections and across longer-term analyses. To accommodate this, targeted MS experiments generally schedule wide target RT windows (&gt;1 min) or rely on heavy labeled peptides to trigger acquisition of endogenous peptides. Both greatly limit the number of quantifiable targets per assay. To overcome this, we utilize adaptive RT (aRT) alignment for live correction of RTs together with the speed and sensitivity of the <a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/products/1959"><strong>Orbitrap Astral Zoom MS</strong></a>. This allows reproducible tracking of &gt;2000 targets with narrow RT windows, high throughputs, such as 300 samples per day (SPD), and hybrid workflows combining targeting, of up to 1000 targets, and discovery.</p><h4><strong>Materials and methods&nbsp;</strong></h4><h5><strong>LC-MS Experiments&nbsp;</strong></h5><p>Experiments were performed using Thermo Scientific Pierce HeLa protein digest resuspended in 0.1% TFA, or individual or pooled human plasma samples. Peptides were separated using a 15 cm Thermo Scientific PepMap analytical columns (150 µm inner diameter) and the <a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/products/398"><strong>Thermo Scientific Vanquish Neo UHPLC system</strong></a> at throughputs of 60 or 300 SPD. The liquid chromatography (LC) system was interfaced with the <a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/products/1959"><strong>Orbitrap Astral Zoom MS.</strong></a> Reference runs using MS1 and/or data-independent-acquisition (DIA) scans were acquired to obtain reference aRT spectra, and to determine target RTs. For aRT alignment, methods consisting of aRT MS1 and/or DIA scans, as well as targeted MS2 scans with RT correction were optimized. For hybrid DIA workflows, we used aRT MS1 scans for reference acquisition and alignment, combined with narrow window DIA and a targeted MS2 experiment. Observed RT shifts were caused by natural column degradation, sample matrix effects or when indicated induced by variation in gradient flow rate and buffer composition.&nbsp;</p><h5><strong>Data analysis&nbsp;</strong></h5><p>Reference DIA data was analyzed using Spectronaut. Targets lists were then generated from the search output by selecting varying number of targets evenly distributed across the RT dimension. Target RTs for smaller scale assays were manually validated and exported from Skyline, while target RTs for large scale assays were exported from Spectronaut. For alignment runs, DIA and PRM data were analyzed using Spectronaut and Skyline respectively.</p><h4><strong>Conclusions&nbsp;</strong></h4><ul><li>Adaptive RT alignment on Orbitrap Astral Zoom MS shows robustness against various sources of RT shifts and has high target recovery even for thousands of targets&nbsp;</li><li>aRT enables the use of target acquisition windows &lt;15 s for robust targeting at high throughput&nbsp;</li><li>aRT Hybrid DIA allows targeting of up to 1000 targets with minimal cost to DIA depth</li></ul><h3><strong>5. Waters Corporation / ASMS: BOOSTING SENSITIVITY IN TOP-DOWN PROTEIN STUDIES USING CYCLIC IMS-ENABLED WIDEBAND ENHANCEMENT</strong></h3><ul><li>Poster</li><li><a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/labrulez-bucket-strapi-h3hsga3/720009471_51f2e8b546.pdf"><strong>Full PDF for download</strong></a></li></ul><div><div><section><div><div><div><div><div><div><p>The poster evaluates <strong>Wideband Enhancement (WBE)</strong> as a way to improve sensitivity in top-down protein mass spectrometry. In top-down workflows, intact proteins are fragmented directly, but the signal is distributed across multiple charge states, isotope envelopes, and numerous product ions, which can limit MS/MS sensitivity. WBE addresses this problem by synchronizing ion packets leaving the ion mobility region with the time-of-flight pusher, thereby increasing the number of ions that reach the detector within a selected <i>m/z</i> range.</p><p>Experiments were performed using a <a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/products/2434"><strong>Waters Cyclic IMS P20 Mass Spectrometer</strong></a> equipped with an <strong>electron capture dissociation cell</strong>. Samples were introduced by direct infusion in positive electrospray ionization mode. Two proteins were studied under denaturing conditions: bovine ubiquitin, approximately 8.5 kDa, and bovine carbonic anhydrase II, approximately 29 kDa. Single-pass and bypass ion mobility measurements were carried out with and without WBE, using both <strong>collision-induced dissociation (CID)</strong> and <strong>electron capture dissociation (ECD)</strong>. Data were acquired in <a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/products/635"><strong>MassLynx</strong></a><strong> v4.2</strong> and processed using <strong>DriftScope</strong> and the <strong>waters_connect UNIFI peptide-mapping workflow</strong>.</p><p>For ubiquitin, WBE substantially increased the intensity of CID- and ECD-derived fragment ions. Improvements of up to tenfold were observed across the c- and z-ion series, while bypass ion mobility mode produced the largest overall sensitivity gain. The results indicate that WBE can enhance fragment detection while retaining the benefits of cyclic ion mobility separation and without sacrificing mass-spectral resolution.</p><p>The larger carbonic anhydrase II protein also showed marked improvements. CID fragment signals increased by up to tenfold for lower-<i>m/z</i> ions and about eightfold for higher-<i>m/z</i> fragments. Because ECD is generally less efficient than CID, the benefit of WBE was especially evident in ECD experiments: sequence coverage increased from <strong>54% without WBE to 70% with WBE</strong> in bypass mode. The authors conclude that combining WBE with complementary CID and ECD fragmentation on the Cyclic IMS P20 enables more sensitive and comprehensive top-down protein characterization.</p></div></div></div></div></div></div></section></div></div>

News from LabRulezLCMS Library - Week 33, 2026

Article

Product

Application

Academy

Science and research

Poster

The Czech Advanced Technology and Research Institute (CATRIN) at Palacký University is a cutting-edge scientific hub dedicated to advancing research in the fields of nanotechnology, biotechnology, and biomedicine. At its core, CATRIN boasts outstanding scientific teams, featuring international researchers. We emphasize interdisciplinarity, foster global collaborations, and work to translate our research findings into practical applications.

CATRIN

Everything from the world of analytical chemistry in one place. We connect people in solving their problems. At Labrulez you will find all the necessary information easily, quickly and clearly. Stop searching and start finding.

LabRulez

Based in Berlin, KNAUER is a medium-sized, owner-managed company that has been serving the sciences since 1962. We develop and manufacture scientific instruments of superior quality for liquid chromatography, including: Analytical HPLC/UHPLC, Preparative HPLC, Fast protein liquid chromatography (FPLC), Multi-column chromatography/Simulated moving bed (SMB) chromatography. High pressure dosing, in-line detection systems, and osmometry are additional business areas.

KNAUER

Bioanalytic Development of LC-MS/MS method

We take an individual and factual approach to developing a new method and performing analyses.

<h4><strong>An experienced partner in the LC-MS/MS analysis industry</strong></h4><p>If you are interested in performing quantitative, qualitative or mixed analysis using LC-MS/MS analytical methods but lack such capabilities due to lack of equipment, “know-how” or are looking for a team to perform analysis for a project - you have come to the right place. We work on <a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/companies/315"><strong>equipment</strong></a> with high sensitivity, speed and specificity. Our team is a group of specialists with comprehensive experience in the industry. The LabExperts <a target="_blank" rel="noopener noreferrer" href="https://labexperts.com.pl/zespol/">team</a> has participated in many scientific projects, R&amp;D and commercial implementations. We take an individual and factual approach to developing a new method and performing analyses:</p><ul><li>In the first stage, we get to know your requirements - to align expectations with capabilities;</li><li>Next, we will form and approve the framework for cooperation, ensuring the observance of confidentiality by signing a non-disclosure agreement (NDA);</li><li>We will plan together and, for our part, meet the deadlines for the completion of the set tasks;</li><li>We will perform preliminary studies, after which we will decide together whether and to what extent we continue to develop the methods;</li><li>We will handle the development of the sample preparation procedure;</li><li>We will select the best parameters for the LC-MS/MS analytical method;</li><li>The analytical method along with the sample preparation procedure will be validated according to generally accepted guidelines and standards, or we will establish the required acceptance criteria and ranges for your needs;</li><li>We will perform LC-MS/MS analyses of a series of samples;</li><li>We will provide results in the form of easy-to-read reports at each required stage;</li><li>We will evaluate the feasibility and assist in the transfer of the method to your laboratory.</li></ul><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Bioanalytic_Bioanalytic_Development_of_LC_MS_MS_method_2594c9fd54.jpg" alt="Bioanalytic: Bioanalytic Development of LC-MS-MS method." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Bioanalytic_Bioanalytic_Development_of_LC_MS_MS_method_2594c9fd54.jpg 234w," sizes="100vw" width="1536" height="1024"></p><p>Pricing and the framework for cooperation are always determined individually depending on the scope of the study. This is influenced by the amount of work and the complexity of the development of the sample preparation procedure and analytical method and routine analytics. As a rule, the final price consists of three separately priced elements: the cost of preliminary studies, method validation, and the cost of analysis of one sample within a series of analyses.</p><p>Don't beat your head against the wall - we will help you achieve your goal!</p><p>We look forward to working with you!</p>

Bioanalytic Individual LC-MS/MS training

We are ready to prepare customized LC-MS/MS training for the needs of participants.

<h4><strong>BASIC INFORMATION</strong></h4><ul><li>TITLE: <strong>Customized LC-MS/MS Training</strong></li><li>CATEGORY: <strong>LC-MS/MS training</strong></li><li>LOCATION: <strong>Lodz, PL</strong> or other</li><li>TRAINING TIME: <strong>1-5 days</strong> or more</li><li>NUMBER OF PARTICIPANTS: <strong>4-8 people</strong></li><li>EQUIPMENT:<strong> SCIEX QTRAP/QTOF LC-MS/MS</strong></li></ul><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Bioanalytic_Bioanalytic_Individual_LC_MS_MS_training_8a73408c56.jpg" alt="Bioanalytic: Bioanalytic Individual LC-MS-MS training." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Bioanalytic_Bioanalytic_Individual_LC_MS_MS_training_8a73408c56.jpg 245w," sizes="100vw" width="1536" height="930"></p><h4><strong>TRAINING DESCRIPTION</strong></h4><p>Customized LC-MS/MS training we are ready to prepare for the needs of participants, discussing:</p><ul><li>theoretical basics of chromatography, mass spectrometry,</li><li>practical operation of the LC-MS/MS system,</li><li>qualitative and quantitative analysis,</li><li>DDA &amp; DIA screening methods,</li><li>“omics” analyses,</li><li>validation of analytical methods,</li><li>solving basic methodological and technical problems,</li><li>software,</li><li>calibration,</li><li>“tips&amp;tricks”,</li><li>etc...</li></ul><p>We offer training for both novices starting the fascinating adventure of LC-MS/MS analytics, as well as for those who are LC-MS/MS users and are interested in expanding and consolidating their knowledge.</p><h4><strong>Framework training program</strong></h4><ul><li>individual LC-MS/MS training is developed according to the customer's demand</li><li>conducting theoretical and/or practical training at the customer's workplace</li><li>carrying out theoretical and/or practical training in a laboratory in Lodz</li></ul>

Bioanalytic Basic LC-MS/MS training

Basic LC-MS/MS training covers the theoretical basics of mass spectrometry and familiarization with the basic operation of the instrument.

<h4><strong>BASIC INFORMATION</strong></h4><ul><li>TITLE: <strong>Basic LC-MS/MS training</strong></li><li>CATEGORY: <strong>LC-MS/MS training</strong></li><li>LOCATION: <strong>Lodz, PL</strong></li><li>TRAINING TIME: <strong>3 days</strong></li><li>NUMBER OF PARTICIPANTS: <strong>up to 5 people</strong></li><li>EQUIPMENT:<strong> SCIEX QTRAP </strong><span class="ts-pf-details-content"><strong>&amp; </strong></span><strong>QTOF LC-MS/MS</strong></li></ul><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Bioanalytic_Bioanalytic_Basic_LC_MS_MS_training_fb1382a55b.jpg" alt="Bioanalytic: Bioanalytic Basic LC-MS-MS training." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Bioanalytic_Bioanalytic_Basic_LC_MS_MS_training_fb1382a55b.jpg 205w," sizes="100vw" width="1293" height="987"></p><h4><strong>TRAINING DESCRIPTION</strong></h4><p>Basic LC-MS/MS training covers the theoretical basics of mass spectrometry and familiarization with the basic operation of the instrument. Basic LC-MS/MS training is primarily aimed at those who are new to LC-MS/MS equipment or who wish to become familiar with this analytical technique. During the training, the following will be presented:</p><ul><li>principles of developing and optimizing quantitative and qualitative methods on low (triple quadrupole, QTRAP) and high resolution (QTOF) instruments,</li><li>basic functionality of the software,</li><li>procedures and objectives of instrument calibration,</li><li>principles of maintaining the cleanliness of mass spectrometer operation.</li></ul><h5><strong>Basic LC-MS/MS training - the program</strong></h5><p><span style="font-size:20px;"><strong>Day I</strong></span></p><p><strong>Theoretical classes:</strong></p><ul><li>Introduction to LC</li><li>Introduction to mass spectrometry (basics)</li><li>Quantitative analysis.</li></ul><p><strong>Practical part:</strong></p><p>QTRAP:</p><ul><li>QTRAP scanning modes on the instrument - Q1, , MS2, MRM, demonstration and discussion on the example of a selected pesticide of the fragmentation spectrum and its fragmentation pathway.</li><li>MRM pair formation (manual, semi-automatic, automatic).</li><li>optimization of ion source parameters -T-conector</li><li>explanation of the main parameters related to LC (column selection, gradient vs isocrat, autosampler)</li><li>LC-MS/MS method setup and optimization</li></ul><p>QTOF:</p><ul><li>manual and automatic optimization of MRM<sup>HR</sup></li><li>ion source and LC parameters (transfer from method developed above)</li></ul><p>Sequence of quantitative analysis on both instruments.</p><p><span style="font-size:20px;"><strong>Day II</strong></span></p><p><strong>Quantitative analysis:</strong></p><ul><li>data processing and analysis,</li><li>reporting - Sciex OS (QTRAP, QTOF).</li><li>Discussion of types of integration algorithms (MQ4, autopeak) and integration parameters.</li><li>independent selection of integration parameters and plotting of master curves.</li><li>independent data analysis and generation of analysis report.</li></ul><p><strong>Qualitative analysis:</strong></p><ul><li>Theoretical introduction - introduction to qualitative analysis:</li><li>qwadrupole modes (Prec/NL),</li><li>trap modes (EMS, EPI, MS3),</li><li>QTOF modes (TOF MS, TOF MS/MS).</li><li>mixed methods,</li><li>targeted vs. untargeted analysis,</li><li>DDA (IDA) - discussion of method development,</li></ul><p><strong>Practical part:</strong></p><ul><li>creation of qualitative methods on QTRAP (Prec-IDA, MRM-IDA), other parameters (LC, ion source) as the previous day,</li><li>LightSight - discussion of operation, creation of MRM-IDA multimethod for selected pesticide and analysis of samples (night).</li><li>QTOF - creation of IDA method, other parameters (LC, ion source) as the previous day.</li></ul><p>Sequence of qualitative analysis overnight.</p><p><span style="font-size:20px;"><strong>Day III</strong></span></p><p><strong>Qualitative data analysis:</strong></p><p>QTRAP:</p><ul><li>Discussion of Prec-IDA, MRM-IDA results - what we see in different types of MS and MS/MS scans. Manual review of data and via IDA Explorer (SciexOS).</li><li>Analysis of results in LightSight, comparison with Prec-IDA, for example - advantages/disadvantages</li></ul><p>QTOF:</p><ul><li>search for potentially present compounds in the sample,</li><li>manual comparison of blank vs. test sample,</li><li>manual by XIC extraction (by sum formula),</li><li>Formula finder - derivation of sum formula on TOF MS data and verification of fragmentation on TOF MS/MS data</li><li>search in IDA Explorer mode - application of filters: similarity, isotopic distribution, mass defect,</li><li>database searching,</li><li>Molecule Profiler - demonstration of software capabilities on selected pesticide example,</li></ul><p><strong>Instrument calibration:</strong></p><ul><li>resolution, mass-to-charge ratio, intensity,</li><li>Q1 and Q3, LIT (MS and MS/MS), TOF MS and TOF-MS/MS</li><li>Automation vs. manual calibration,</li></ul><p><strong>LC-MS/MS application examples</strong></p><p><strong>Summary</strong></p><p><strong>Discussion</strong></p>

Bioanalytic Quantitative LC-MS/MS analysis training

The goal of the training is to introduce users to the procedures and capabilities of the LC-MS/MS system and software to achieve sensitivity, selectivity, precision and reproducibility in quantitative analysis.

<h4><strong>BASIC INFORMATION</strong></h4><ul><li>TITLE: <strong>Quantitative LC-MS/MS analysis training</strong></li><li>CATEGORY: <strong>LC-MS/MS training</strong></li><li>LOCATION: <strong>Lodz, PL</strong></li><li>TRAINING TIME: <strong>3 days</strong></li><li>NUMBER OF PARTICIPANTS: <strong>up to 5 people</strong></li><li>EQUIPMENT:<strong> SCIEX QTRAP LC-MS/MS</strong></li></ul><figure class="image"><img style="aspect-ratio:1200/675;" src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Bioanalytic_Bioanalytic_Quantitative_LC_MS_MS_analysis_training_d13b5d8127.jpg" alt="Bioanalytic: Bioanalytic Quantitative LC-MS-MS analysis training." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Bioanalytic_Bioanalytic_Quantitative_LC_MS_MS_analysis_training_d13b5d8127.jpg 245w," sizes="100vw" width="1200" height="675"></figure><h4><strong>TRAINING DESCRIPTION</strong></h4><p>Quantitative LC-MS/MS analysis - the training is designed for laboratory personnel using LC-MS/MS systems for quantitative analysis. The following topics are covered during the training:</p><ul><li>Triple quadrupole spectrometer calibration</li><li>MRM pair formation and optimization</li><li>Optimization of ion source parameters</li><li>Optimization of chromatographic parameters</li><li>Sample preparation using the example of blood drawn on <a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/products/1283"><strong>Mitra®</strong></a> samplers (VAMS)</li><li>Matrix effect, internal standards (ISTD), matrix curves</li><li>Validation of the quantitative method</li><li>Reporting of results</li></ul><p>The goal of the training is to introduce users to the procedures and capabilities of the LC-MS/MS system and software to achieve sensitivity, selectivity, precision and reproducibility in quantitative analysis while covering the widest possible linear range of concentrations.</p><h3><strong>Training program</strong></h3><h4><span style="font-size:20px;"><strong>Day I</strong></span></h4><h5><strong>Theoretical part:</strong></h5><p>Triple quadrupole structure, ionization, MRM and sMRM scanning, chromatography, multimethods</p><h5><strong>Practical part:</strong></h5><ul><li>Q1/Q3 calibration (10 Da/s), MS/MS - resolution, m/z, intensity.</li><li>MRM pair formation and optimization (manual, semi-automatic, automatic).</li><li>Ion source optimization - T-concector and FIA</li><li>Gradient vs isocrat, dosing, column selection, temperature selection, other important parameters</li><li>Establishment and optimization of LC-MS/MS method.</li><li>How to take care of MS cleanliness - ionization time programming, multi-way valve, cleaning of ion source and instrument front end</li></ul><p>Sample preparation in pure solvent (standard curve, QC, solvent, solvent+ISTD, test samples), overnight analysis sequence.</p><h4><span style="font-size:20px;"><strong>Day II</strong></span></h4><h5><strong>Practical part - preparation of whole blood samples (Mitra®):</strong></h5><ul><li>Preparation of matrix standard curves</li><li>Preparation of quality control (QC) samples in three concentrations</li><li>Preparation of blank samples (without ISTD and with ISTD).</li><li>Preparation of samples for recovery determination (“pre-spiked” and “post-spiked”)</li><li>Preparation of test samples</li></ul><h5><strong>Theoretical part:</strong></h5><ul><li>Samples in the matrix - discussion of the matrix effect and ways to level it.</li><li>Optimization of sample preparation method and LC-MS/MS method</li></ul><h5><strong>Practical part:</strong></h5><ul><li>Sample extraction from Mitra® samplers for LC-MS/MS analysis.</li><li>Sequence of analysis of prepared samples overnight</li></ul><h4><span style="font-size:20px;"><strong>Day III</strong></span></h4><h5><strong>Theoretical part:</strong></h5><ul><li>Validation of the quantitative LC-MS/MS method</li></ul><h5><strong>Practical part:</strong></h5><ul><li>Software - data integration algorithms, signal smoothing, weighing.</li><li>Matrix curves vs curves in solvent, influence of ISTD (Sciex OS).</li><li>Method validation - validation criteria, determination of LOD, LLOQ, ULOQ, linearity, working range of the method, repeatability, recovery.</li><li>Preparation of summary validation reports</li><li>Reporting of results from test samples - report vs export of data to Excel.</li><li>Summary and discussion.</li></ul>

Bioanalytic QTRAP vs QTOF Quality analysis training

The training includes a presentation of the basic differences in application and a comparison of the capabilities of QTRAP and QTOF instruments in qualitative analysis.

<h4><strong>BASIC INFORMATION</strong></h4><ul><li>TITLE: <strong>QTRAP vs QTOF Quality analysis training</strong></li><li>CATEGORY: <strong>LC-MS/MS training</strong></li><li>LOCATION: <strong>Lodz, PL</strong></li><li>TRAINING TIME: <strong>3 days</strong></li><li>NUMBER OF PARTICIPANTS: <strong>up to 5 people</strong></li><li>EQUIPMENT:<strong> SCIEX QTRAP </strong><span class="ts-pf-details-content"><strong>&amp; </strong></span><strong>QTOF LC-MS/MS</strong></li></ul><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Bioanalytic_Bioanalytic_QTRAP_vs_QTOF_Quality_analysis_training_18ca66163f.jpg" alt="Bioanalytic: Bioanalytic QTRAP vs QTOF Quality analysis training." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Bioanalytic_Bioanalytic_QTRAP_vs_QTOF_Quality_analysis_training_18ca66163f.jpg 221w," sizes="100vw" width="1000" height="707"></p><h4><strong>TRAINING COURSE DESCRIPTION</strong></h4><p><strong>QTRAP vs QTOF qualitative analysis</strong> - the training is aimed at people who perform analyses related to substance identification or metabolite search in their research work. The training includes a presentation of the basic differences in application and a comparison of the capabilities of QTRAP and QTOF instruments in qualitative analysis. In addition to the theoretical session, participants will undergo a course in the development and optimization of <strong>DDA (IDA)</strong> (QTRAP &amp; QTOF) and <strong>DIA (SWATH) </strong>(QTOF) methods. Analysis and comparison of results will be conducted using <a target="_blank" rel="noopener noreferrer" href="https://sciex.com/products/software/sciex-os-software">SciexOS</a>, <a target="_blank" rel="noopener noreferrer" href="https://sciex.com/products/software/markerview-software">MarkerView</a>, <a target="_blank" rel="noopener noreferrer" href="https://sciex.com/products/software/lightsight-software">LightSight</a> and <a target="_blank" rel="noopener noreferrer" href="https://sciex.com/products/software/molecule-profiler-software">MoleculeProfiler</a> software.</p><h5><strong>Training program</strong></h5><h4><span style="font-size:20px;"><strong>Day I</strong></span></h4><h5><strong>Theoretical part</strong></h5><ul><li>Principle of operation of MS/MS systems</li><li>Differences and capabilities of QTRAP and QTOF</li><li>Comparison of typical analytical procedures and their application</li><li>DDA (IDA) vs DIA (SWATH) methodology.</li><li>Supporting software - LightSight, Molecule Profiler, ACD Labs, MarkerView</li><li>General principles of compound fragmentation</li></ul><h4><span style="font-size:20px;"><strong>Day II</strong></span></h4><p><strong>Objective - to develop methods to search for metabolites of a known substance.</strong></p><h5><strong>QTRAP</strong></h5><ul><li>Development of IDA screening methods (pMRM, Prec/NL, EMS)</li><li>Optimization of LC-MS/MS IDA method</li><li>Data acquisition</li></ul><h5><strong>QTOF</strong></h5><ul><li>Development and optimization of LC-MS/MS IDA and SWATH methods</li><li>Data acquisition</li></ul><h4><span style="font-size:20px;"><strong>Day III</strong></span></h4><p><strong>Objective - identification and profiling of metabolites of a known substance</strong></p><h5><strong>QTRAP data analysis</strong></h5><ul><li>Manual search and identification</li><li>LightSight, MarkerView</li></ul><h5><strong>QTOF data analysis</strong></h5><ul><li>Search and data analysis methods</li><li>Manual search and identification</li><li>IDA vs SWATH</li><li>Automated search and identification - SciexOS, databases</li><li>Molecule Profiler, MarkerView</li></ul><p>Summary and discussion<br>&nbsp;</p>

Bioanalytic QTOF quantitative and qualitative analysis training

The purpose of the training is to introduce users to the capabilities of the LC-HR-MS/MS (QTOF) system in quantitative and qualitative analysis.

<h4><strong>BASIC INFORMATION</strong></h4><ul><li>TITLE:&nbsp;<strong> QTOF quantitative and qualitative analysis training</strong></li><li>CATEGORY: <strong>LC-MS/MS training</strong></li><li>LOCATION: <strong>Lodz, PL</strong></li><li>TRAINING TIME: <strong>3 days</strong></li><li>NUMBER OF PARTICIPANTS: <strong>up to 5 people</strong></li><li>EQUIPMENT:<strong> SCIEX</strong><span class="ts-pf-details-content"><strong> </strong></span><strong>QTOF LC-MS/MS</strong></li></ul><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Bioanalytic_Bioanalytic_QTOF_quantitative_and_qualitative_analysis_training_af1620ae8c.jpg" alt="Bioanalytic: Bioanalytic QTOF quantitative and qualitative analysis training." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Bioanalytic_Bioanalytic_QTOF_quantitative_and_qualitative_analysis_training_af1620ae8c.jpg 221w," sizes="100vw" width="1000" height="707"></p><h4><strong>TRAINING COURSE DESCRIPTION</strong></h4><p><strong>Qualitative and quantitative analysis of QTOF</strong> - the training is designed for laboratory personnel using <a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/library?instrumentationItems=30&amp;mainauthorItems=55"><strong>high-resolution (HR) LC-MS/MS</strong></a> systems for qualitative and quantitative analysis. The following topics are covered during the training:</p><ul><li><strong>QTOF spectrometer calibration</strong></li><li><strong>Targeted and untargeted methods</strong></li><li><strong>Quantitative analysis (TOF MS, MRMhr)</strong></li><li><strong>Qualitative and quantitative analysis - DDA (IDA) vs DIA (SWATH) methodology</strong></li><li><strong>Software and its use in high-resolution data analysis</strong></li><li><strong>Reporting of results</strong></li></ul><p>The purpose of the training is to introduce users to the capabilities of the LC-HR-MS/MS (QTOF) system in quantitative and qualitative analysis. To point out the advantages of high resolution in identifying or confirming the presence of substances in a sample and also the strengths of modern QTOF systems in parallel quantitative analysis.</p><h3><strong>Training program</strong></h3><h4><span style="font-size:20px;"><strong>Day I</strong></span></h4><h5><strong>Theoretical part</strong></h5><ul><li>The principle of operation and capabilities of the QTOF system</li><li>Comparison of typical analytical procedures and their application</li><li>Targeted and quantitative analysis (TOF MS vs MRMhr)</li><li>DDA (IDA) vs DIA (SWATH) methodology.</li><li>Supporting software - Molecule Profiler, ACD Labs, MarkerView</li><li>General principles of compound fragmentation</li></ul><h4><span style="font-size:20px;"><strong>Day II</strong></span></h4><p><strong>Objective</strong> - to develop methods for quantitative analysis, confirmation or search for metabolites of a known substance, general characterization of the sample.</p><h5><strong>Practical part:</strong></h5><ul><li>Creation and optimization of the MRMhr targeted method</li><li>Creation and optimization of a non-targeted method IDA</li><li>Creation and optimization of the non-targeted method SWATH</li><li>Data acquisition (overnight)</li></ul><h4><span style="font-size:20px;"><strong>Day III</strong></span></h4><p><strong>Objective</strong> - quantitative analysis, confirmation of the presence or identification of metabolites of a known substance, general characterization of the sample (identification of compounds present in the sample), profiling.</p><h5><strong>Practical part:</strong></h5><ul><li>Quantitative analysis - TOF MS vs MRMhr</li><li>Methods for searching and analyzing data</li><li>IDA vs SWATH data</li><li>Manual search and identification</li><li>Automated search and identification - SciexOS, databases</li><li>Molecule Profiler, MarkerView</li><li>Summary and discussion</li></ul>

Thermo Scientific Orbitrap Exploris MX Mass Detector

Accelerate biopharmaceutical late-stage development, commercialization, manufacturing, and quality control (QC) by easily and reliably obtaining compliance-ready, high-quality mass information. 

<p><strong>Accelerate biopharmaceutical late-stage development, commercialization, manufacturing, and quality control (QC) by easily and reliably obtaining compliance-ready, high-quality mass information.&nbsp;</strong></p><p>The <strong>Thermo Scientific Orbitrap Exploris MX mass detector</strong> is the perfect fit for deploying routine mass monitoring.</p><p>In addition to high-resolution mass detection provided by Thermo Scientific Orbitrap mass analyzers, this fit for purpose system is simple to operate and compliance ready with <a target="_blank" rel="noopener noreferrer" href="https://www.thermofisher.com/cz/en/home/industrial/chromatography/chromatography-learning-center/chromeleon-cds-information/cds-mass-spectrometry-software.html">Thermo Scientific Chromeleon chromatography data system (CDS) software</a>. Consistent performance between the <a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/products/402">Thermo Scientific Orbitrap Exploris 240 mass spectrometer</a> and Orbitrap Exploris MX detector ensures seamless method transfer from development to QC.</p><h4><strong>High-resolution accurate-mass (HRAM) data provide utmost confidence for:</strong></h4><ul><li>Sequence confirmation</li><li>Peptide monitoring</li><li>Intact protein analysis (from small to large molecules, reduced mAbs, and intact mAbs under native and denaturing conditions)</li><li>Glycan profiling</li><li>Oligonucleotide and oligonucleotide impurity mass confirmation</li></ul><h3><strong>Highlights of Orbitrap Exploris MX mass detector&nbsp;</strong></h3><div colctrl-id="5-1"><div><div container-id="cq-colctrl-lt0" comp-id="A1"><div><h4><strong>Simplicity for scientists and technicians</strong></h4><p>Simplify tasks and reduce errors for more ‘right first time’ analyses with Chromeleon CDS, with built in methods, control, data processing, and management. The suite of smart tools makes your work faster and easier, while ensuring reproducible, high-quality mass monitoring.</p></div></div></div><div><div container-id="cq-colctrl-lt0" comp-id="B1"><div><h4><strong>Accelerate productivity</strong></h4><p>High uptime boosts productivity. Rapid “set and forget” calibration procedures provide consistent mass stability for at least four weeks at prescribed conditions. Instrument status monitoring and optimized pressure control alert users when maintenance is needed, avoiding unnecessary downtime and repeat analyses.</p></div></div></div></div><div colctrl-id="6-1"><div><div container-id="cq-colctrl-lt0" comp-id="A1"><div><h4><strong>Compliance for competitive advantage</strong></h4><p>Designed for data integrity, data security, and compliance, fully scalable Chromeleon CDS provides comprehensive technical controls to give you a competitive edge in meeting global regulatory requirements.</p></div></div></div><div><div container-id="cq-colctrl-lt0" comp-id="B1"><div><h4><strong>Consistent results deliver confidence</strong></h4><p>Robust and reliable performance from system to system and site to site ensure confidence in results used to make critical decisions. Industry-proven Orbitrap technology, now purposely designed for mass monitoring in future-proof lab operations.</p></div></div></div></div><div colctrl-id="7-1"><div><div container-id="cq-colctrl-lt0" comp-id="A1"><div><h4><strong>Seamless method transfer to MAM</strong></h4><p>Easy transfer of sophisticated Multi-Attribute Method (MAM) assays from development to manufacturing bridges the gap between development and QC.</p></div></div></div><div><div container-id="cq-colctrl-lt0" comp-id="B1"><div><h4><strong>Dedicated, global support team</strong></h4><p>The dedicated, global Thermo Scientific MAM team maximizes your productivity and confidence with installation, training, and fast service and support. Standardized system performance evaluation test (SET) and installation qualification/operational qualification (IQ/OA) thoroughly assess system performance with comprehensive installation and post-maintenance acceptance criteria.</p><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Thermo_Scientific_Orbitrap_Exploris_MX_Mass_Detector_4_2ad72d9b21.jpg" alt="Orbitrap Exploris MX mass stability: mass deviation relative to first observed mass in parts-per-million for 9 BSA tryptic peptides monitored over a period of 6 weeks using the new internal recalibration option. Each data point is an average of 19 individual injections." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Thermo_Scientific_Orbitrap_Exploris_MX_Mass_Detector_4_2ad72d9b21.jpg 184w," sizes="100vw" width="1240" height="1052"></p><h3><strong>Accelerate MAM deployment&nbsp;</strong></h3><h4><strong>Seamless knowledge and method transfer</strong></h4><p>Benefit from the end-to-end Thermo Scientific MAM 2.0 workflow approach to comprehensive characterization and monitoring of biotherapeutics from development to commercialization. <a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/products/402">Orbitrap Exploris 240</a> mass spectrometer methods, established during development, seamlessly transfer to the Orbitrap Exploris MX detector in manufacturing or by contract partners. In addition to full integration into the high-resolution MAM 2.0 workflow, the usability and low cost of the Orbitrap Exploris MX detector make it the perfect fit for high-throughput QC and contract labs.</p><p>Users can identify and evaluate relevant product quality attributes (PQAs) via high-throughput analytics provided by Thermo Scientific BioPharma Finder software. Connected labs can access data from development through QC using MAM 2.0 powered by Thermo Scientific Ardia Platform. And, PQAs can be monitored throughout the entire drug development process using the MAM target peptide workbook built from the list of identified peptides upon incorporation into a Chromeleon processing method. The processing method along with the instrument method, injection sequence, view setting and report template can be stored into a MAM eWorkflow for easy and direct transfer to QC via Chromeleon CDS Enterprise software.</p><div><h5><strong>MAM 2.0 offers:</strong></h5><ul><li>Fast decision making based on high-confidence PQAs obtained using industry-leading HRAM Orbitrap technology</li><li>Seamless knowledge and method transfer across instruments, departments, and sites using compliance-ready, Chromeleon CDS enterprise software, and Ardia Platform</li></ul><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Thermo_Scientific_Orbitrap_Exploris_MX_Mass_Detector_cd7474e7ed.jpg" alt="Thermo Scientific Orbitrap Exploris MX Mass Detector" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Thermo_Scientific_Orbitrap_Exploris_MX_Mass_Detector_cd7474e7ed.jpg 245w," sizes="100vw" width="1170" height="504"></p><blockquote><p><strong>Thermo Scientific MAM 2.0</strong></p><p>The end-to-end MAM solution for a fully connected, compliance-ready platform. MAM 2.0 includes everything you need to bring your next biotherapeutic candidate straight through to breakthrough.</p><ul><li><a target="_blank" rel="noopener noreferrer" href="https://www.thermofisher.com/cz/en/home/industrial/pharma-biopharma/biopharmaceutical-analytical-testing/multi-attribute-method.html"><strong>Learn more</strong></a></li></ul></blockquote></div></div></div></div></div>

Thermo Scientific Orbitrap Exploris Mass Spectrometers

Discover which Orbitrap Exploris mass spectrometry system will meet your business needs for more efficient screening, identification, confirmation and quantitation.

<p><strong>Benefit from record-setting performance. With built-in intelligence, sensitivity and selectivity; the Thermo Scientific Orbitrap Exploris mass spectrometry is quantitative high-resolution, accurate-mass (HRAM) liquid chromatography mass spectrometry (LC-MS) for analysis depth to the lowest levels with high quantitative accuracy and precision to meet your exact analytical requirements. Scale up your research and translate your results more rapidly and simply than ever before.</strong></p><h3><strong>Why choose an Orbitrap Exploris mass spectrometer for your laboratory?</strong></h3><p>Discover which Orbitrap Exploris mass spectrometry system will meet your business needs for more efficient screening (including targeted, suspected and nontargeted), identification, confirmation and quantitation.</p><h4><a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/products/1490"><strong>Orbitrap Exploris MX mass detector</strong></a></h4><p>Accelerate biopharmaceutical commercialization, manufacturing and quality control by easily and reliably obtaining compliance-ready, high-quality mass information with the Thermo Scientific Orbitrap Exploris MX Mass Detector.</p><p><strong>Typical applications: </strong>Biopharmaceutical characterization and QC, intact protein analysis, Multi-Attribute Method (MAM), oligonucleotides, pharmaceuticals</p><p><strong>Analyses: </strong>Targeted / non-targeted screening (MS), Unknown Identification (MS), Absolute and Relative Quantitation (MS)</p><p><strong>Resolution powers: </strong>180,000</p><p><strong>Mass range: </strong>m/z 40–3,000 up to m/z 8,000 with BioPharma option</p><p><strong>Scan speed: </strong>22Hz</p><p><strong>Sources &amp; Flexibility: </strong><a target="_blank" rel="noopener noreferrer" href="https://www.thermofisher.com/cz/en/home/industrial/mass-spectrometry/liquid-chromatography-mass-spectrometry-lc-ms/lc-ms-systems/orbitrap-lc-ms/orbitrap-exploris-mass-spectrometers.html#small">AP-SMALDI<sup>5</sup>&nbsp;AF source for MS Imaging</a></p><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Thermo_Scientific_Orbitrap_Exploris_MX_Mass_Detector_2_a84ed39672.jpg" alt="Thermo Scientific Orbitrap Exploris MX Mass Detector" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Thermo_Scientific_Orbitrap_Exploris_MX_Mass_Detector_2_a84ed39672.jpg 169w," sizes="100vw" width="1696" height="1575"></p><h4><a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/products/403"><strong>Orbitrap Exploris 120 Mass Spectrometer&nbsp;</strong></a></h4><p>Benefit from best in class high-resolution accurate mass for confident testing, screening and quantification of small molecules with the Thermo Scientific Orbitrap Exploris 120 Mass Spectrometer.&nbsp;&nbsp;</p><p><strong>Typical applications: </strong>Food and environmental testing, metabolomics, forensic toxicology, clinical research, pharmaceuticals</p><p><strong>Analyses: </strong>Targeted / non-targeted screening (MS), Unknown Identification (MS), Confirmation (MS/MS), Absolute and Relative Quantitation (MS and/or MS<sup>2</sup>)</p><p><strong>Resolution powers: </strong>120,000</p><p><strong>Mass range: </strong><i>m/z</i>&nbsp;40 – 3,000</p><p><strong>Scan speed: </strong>22Hz</p><p><strong>Sources &amp; Flexibility: </strong><a target="_blank" rel="noopener noreferrer" href="https://www.thermofisher.com/order/catalog/product/IQLAAEGABBFAGOMZZZ">APCI/APPI Combination</a>, <a target="_blank" rel="noopener noreferrer" href="https://www.thermofisher.com/cz/en/home/industrial/mass-spectrometry/liquid-chromatography-mass-spectrometry-lc-ms/lc-ms-systems/orbitrap-lc-ms/orbitrap-exploris-mass-spectrometers.html#small">AP-SMALDI<sup>5</sup>&nbsp;AF source for MS Imaging</a></p><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Thermo_Orbitrap_Exploris_120_Mass_Spectrometer_3_77e4e07555/Thermo-Orbitrap-Exploris-120-Mass-Spectrometer-3.jpg" alt="Thermo Orbitrap Exploris 120 Mass Spectrometer " srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Thermo_Orbitrap_Exploris_120_Mass_Spectrometer_3_77e4e07555/thumbnail_Thermo-Orbitrap-Exploris-120-Mass-Spectrometer-3.jpg 171w," sizes="100vw" width="938" height="856"></p><h4><a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/products/402"><strong>Orbitrap Exploris 240 Mass Spectrometer&nbsp;</strong></a></h4><p>Get unmatched confidence for discovery and identification, with application versatility and operational simplicity with the Thermo Scientific Orbitrap Exploris 240 Mass Spectrometer.&nbsp;</p><p><strong>Typical applications: </strong>Biopharmaceutical characterization, proteomics, metabolomics, lipidomics, food and environmental research, clinical research</p><p><strong>Analyses: </strong>Targeted / non-targeted screening (MS), Unknown Identification (MS), Confirmation (MS/MS), Absolute and Relative Quantitation (MS and/or MS<sup>2</sup>)</p><p><strong>Resolution powers: </strong>240,000</p><p><strong>Mass range: </strong><i>m/z</i>&nbsp;40 - 6000, (up to 8000 with BioPharma option)</p><p><strong>Scan speed: </strong>30Hz</p><p><strong>Sources &amp; Flexibility: </strong><a target="_blank" rel="noopener noreferrer" href="https://www.thermofisher.com/us/en/home/industrial/mass-spectrometry/liquid-chromatography-mass-spectrometry-lc-ms/lc-ms-accessories/field-asymmetric-ion-mobility-spectrometry-faims.html">FAIMS Pro Duo interface</a>, <a target="_blank" rel="noopener noreferrer" href="https://www.thermofisher.com/order/catalog/product/IQLAAEGABBFAGOMZZZ">APCI/APPI Combination</a>, <a target="_blank" rel="noopener noreferrer" href="https://www.thermofisher.com/order/catalog/product/ES081">EASY-Spray Source</a>, <a target="_blank" rel="noopener noreferrer" href="https://www.thermofisher.com/order/catalog/product/ES071">Nanospray Flex Ion Sources</a>&nbsp;, <a target="_blank" rel="noopener noreferrer" href="https://www.thermofisher.com/cz/en/home/industrial/mass-spectrometry/liquid-chromatography-mass-spectrometry-lc-ms/lc-ms-systems/orbitrap-lc-ms/orbitrap-exploris-mass-spectrometers.html#small">AP-SMALDI<sup>5</sup>&nbsp;AF source for MS Imaging</a></p><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Thermo_Orbitrap_Exploris_240_Mass_Spectrometer_2_a5085c34b0/Thermo-Orbitrap-Exploris-240-Mass-Spectrometer-2.jpg" alt="Thermo Orbitrap Exploris 240 Mass Spectrometer " srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Thermo_Orbitrap_Exploris_240_Mass_Spectrometer_2_a5085c34b0/thumbnail_Thermo-Orbitrap-Exploris-240-Mass-Spectrometer-2.jpg 175w," sizes="100vw" width="945" height="841"></p><h4><a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/products/401"><strong>Orbitrap Exploris 480 Mass Spectrometer&nbsp;</strong></a></h4><p>Achieve next-level insights, exploratory and targeted quantitation, next-gen power, performance, and ease of use with the Thermo Scientific Orbitrap Exploris 480 Mass Spectrometer.</p><p><strong>Typical applications: </strong>Shotgun proteomics (LFQ DIA, DDA), single cell proteomics, TMT multiplexing, targeted peptide quantification, metabolomics, biopharmaceutical applications (including HCP and protein characterization).</p><p><strong>Analyses: </strong>Targeted / non-targeted screening (MS), Unknown Identification (MS), Confirmation (MS/MS), Absolute and Relative Quantitation (MS and/or MS<sup>2</sup>)</p><p><strong>Resolution powers: </strong>480,000</p><p><strong>Mass range: </strong><i>m/z</i>&nbsp;40 - 6000, (up to 8000 with BioPharma option)</p><p><strong>Scan speed: </strong>40Hz</p><p><strong>Sources &amp; Flexibility: </strong><a target="_blank" rel="noopener noreferrer" href="https://www.thermofisher.com/us/en/home/industrial/mass-spectrometry/liquid-chromatography-mass-spectrometry-lc-ms/lc-ms-accessories/field-asymmetric-ion-mobility-spectrometry-faims.html">FAIMS Pro Duo interface</a>, <a target="_blank" rel="noopener noreferrer" href="https://www.thermofisher.com/order/catalog/product/IQLAAEGABBFAGOMZZZ">APCI/APPI Combination</a>, <a target="_blank" rel="noopener noreferrer" href="https://www.thermofisher.com/order/catalog/product/ES081">EASY-Spray Source</a>, <a target="_blank" rel="noopener noreferrer" href="https://www.thermofisher.com/order/catalog/product/ES071">Nanospray Flex Ion Sources</a>&nbsp;, <a target="_blank" rel="noopener noreferrer" href="https://www.thermofisher.com/cz/en/home/industrial/mass-spectrometry/liquid-chromatography-mass-spectrometry-lc-ms/lc-ms-systems/orbitrap-lc-ms/orbitrap-exploris-mass-spectrometers.html#small">AP-SMALDI<sup>5</sup>&nbsp;AF source for MS Imaging</a></p><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Thermo_Orbitrap_Exploris_480_Mass_Spectrometer_2_3d91c9b5b1/Thermo-Orbitrap-Exploris-480-Mass-Spectrometer-2.jpg" alt="Thermo Orbitrap Exploris 480 Mass Spectrometer " srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Thermo_Orbitrap_Exploris_480_Mass_Spectrometer_2_3d91c9b5b1/thumbnail_Thermo-Orbitrap-Exploris-480-Mass-Spectrometer-2.jpg 235w," sizes="100vw" width="880" height="584"></p>

Waters ACQUITY QDa II Mass Detector

The ACQUITY™ QDa™ II Mass Detector enhances efficiency and productivity in separations workflows with advanced technology.

<h3><strong>Advancing the Power of Mass Detection</strong></h3><ul><li><strong>Identify Everything in Your Sample with Complete Confidence&nbsp;</strong></li></ul><h5><strong>Information-rich mass spectral data starts here&nbsp;</strong></h5><p><strong>Built on a solid foundation of groundbreaking technology, the ACQUITY™ QDa™ II Mass Detector provides even more efficiency and productivity of separations workflows, providing information-rich mass spectral data, ensuring compliance, and regulatory certainty.</strong></p><h4><strong>SEE MORE COMPOUNDS</strong></h4><p>Greater compound coverage from an extended mass range to support small molecules, large molecules and new modalities. A complementary technique to analog detection for chemicals with no chromophores, ACQUITY QDa II Mass Detector enables you to confirm the identity and purity of more compounds with the power of mass detection.</p><h4><strong>ANALYZE MORE SAMPLES</strong></h4><p>Robust, reliable and requiring no sample-specific adjustment. Simply power on to make informed decisions with the full picture of what’s in your sample. Minimal maintenance along with system performance monitoring ensures maximum uptime.&nbsp;</p><h4><strong>HAVE MORE CONFIDENCE IN THE ROBUSTNESS OF YOUR SEPARATIONS</strong></h4><p>The addition of mass detection to your method development workflow enables fast peak tracking and greater confidence in peak purity, for more robust separations. Combined with industry-leading AQbD method development software, your lab can achieve unprecedented MS peak tracking functionality with the ACQUITY QDa II Mass Detector.</p><h4><strong>STRIVE FOR MORE SUSTAINABILITY</strong></h4><p>Achieve up to a 70% reduction in energy consumption and heat output compared to other vendor’s MS systems. The ACT Environmental Impact Factor Label<strong>*</strong> -- available and published by My Green Lab – provides clear, third-party verified information about the environmental impact of laboratory products, enabling you to make the most sustainable decisions for your organization.</p><p><strong>* </strong>ACT - About (<a target="_blank" rel="noopener noreferrer" href="https://www.mygreenlab.org/">mygreenlab.org</a>)</p><h3><strong>Continuing to Separate Beyond Question</strong></h3><ul><li><strong>Ensure your laboratory is prepared for the future</strong></li></ul><h4><strong>MORE PERFORMANCE</strong></h4><p>Plug and play performance with its intuitive design, complementary to optical detection with the robustness to handle all of your analyses. Working in harmony with your chromatography, it’s pre-optimized to work with your samples, without the sample-specific or user adjustments typical of traditional mass spectrometers.</p><h4><strong>MORE EFFICIENCY</strong></h4><p>The intuitive design of the ACQUITY QDa II Mass Detector provides additional data without cost or complexity. The most information you can get out of your separation for complete characterization. In synergy with optical detection, you can significantly reduce the chance that a sample component will go undetected helping to mitigate risk and ensuring a sound return on your investment.</p><h4><strong>MORE SUSTAINABILITY</strong></h4><p>Purposeful innovation, a stackable mass detector that seamlessly integrates into existing workflows resulting in less bench space and more energy efficient. Minimal cleaning and routine maintenance ensuring maximum uptime. Compatible with green solvents ensuring reduced environmental impact.</p><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Waters_ACQUITY_Q_Da_II_Mass_Detector_3_f98f84c080.jpg" alt="Waters ACQUITY QDa II Mass Detector." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Waters_ACQUITY_Q_Da_II_Mass_Detector_3_f98f84c080.jpg 234w," sizes="100vw" width="1024" height="682"></p><ul><li>Simply power on, and you’re ready to go</li><li>Zero user set-up optimization, calibration, or adjustment</li><li>Run your analyses − it just works</li><li>When you’re done, just power-off</li></ul><h3><strong>The Perfect Partner</strong></h3><h4><strong>Designed for compatibility with your chromatography&nbsp;</strong></h4><p>Meet all your chromatography requirements with Waters chromatography systems, including our dependability and flexibility ranges of HPLC systems, performance range of UPLC systems, and our purification and specialty systems.</p><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Waters_ACQUITY_Q_Da_II_Mass_Detector_The_Perfect_Partner_Designed_for_compatibility_with_your_chromatography_1042adecb0.jpg" alt="Waters ACQUITY QDa II Mass Detector: The Perfect Partner. Designed for compatibility with your chromatography." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Waters_ACQUITY_Q_Da_II_Mass_Detector_The_Perfect_Partner_Designed_for_compatibility_with_your_chromatography_1042adecb0.jpg 189w," sizes="100vw" width="1299" height="1074"></p><h3><strong>Effortless Integration</strong></h3><h4><strong>Achieve more with flexible software solutions</strong></h4><p>Process, Interpret, visualize, and compare the most complex data, and turn it into meaningful information quickly and simply. With integrated optical and mass detector data processing workflows, you can also analyze mass spectral data in the same way as you do photo diode array (PDA) data.</p><p>The ACQUITY QDa II Mass Detector is fully compatible with Masslynx™ Software and its suite of data processing application managers. Empower™ Software, our industry-leading chromatography data software platform is also fully compatible providing the ultimate flexibility.</p><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Waters_ACQUITY_Q_Da_II_Mass_Detector_Effortless_Integration_Achieve_more_with_flexible_software_solutions_4fd6e9f0d8.jpg" alt="Waters ACQUITY QDa II Mass Detector: Effortless Integration. Achieve more with flexible software solutions." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Waters_ACQUITY_Q_Da_II_Mass_Detector_Effortless_Integration_Achieve_more_with_flexible_software_solutions_4fd6e9f0d8.jpg 245w," sizes="100vw" width="1397" height="422"></p><h4><strong>EMPOWER CHROMATOGRAPHY DATA SYSTEM (CDS)</strong></h4><p>Simplifying the way you collect, manage, and report chromatography test results is critical to your lab efficiency. With a suite of flexible functionalities that grows to meet your changing quality, business, and laboratory needs, Empower CDS has been purposefully designed to help you address your most demanding chromatographic, regulatory, and networking challenges.</p><h4><strong>EMPOWER LAB MANAGEMENT SYSTEM (LMS)</strong></h4><p>Automating your lab workflow, from sample creation to data archival, is critical for maintaining data integrity and enhancing productivity. Manage your data all from a single, simple dashboard for an integrated approach to your product lifecycle management so you can reduce transcription errors, increase efficiency, and maximize data durability.</p><h4><a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/products/1544"><strong>waters_connect™</strong></a><strong> SYSTEM MONITORING</strong></h4><p>Monitor all Empower Software-controlled chromatography systems remotely, at any time, from anywhere. Dynamically plan laboratory activities, monitor the status of your laboratory and maximize productivity. Troubleshoot and respond to errors faster, increasing system uptime.</p><h3><strong>Identify More in Released Glycan Analysis</strong></h3><h4><strong>Unlock the full potential of your QC glycan testing</strong></h4><p>While non-MS methods offer routine QC capabilities, they fall short in delivering the detailed characterization, crucial for biopharmaceutical development. The ACQUITY QDa II Mass Detector with extended mass range revolutionizes routine analysis, offering accurate QC monitoring of N-glycan species with speed and precision. Coupled with Waters chromatography systems, enjoy enhanced MS response with uniform charge state for accurate quantitation, enabling scrutiny of released N-glycan profiles in under 40 minutes per sample. Complement fluorescence (FLR) detection and embrace reproducible, quantitative separation for rapid decision-making.</p><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Waters_ACQUITY_Q_Da_II_Mass_Detector_Selected_Ion_Recording_SIR_chromatogram_of_doubly_charged_components_of_a_glycan_sample_Highlighted_additional_components_identified_s_a_result_of_the_extended_mass_range_ac0160e74d.jpg" alt="Waters ACQUITY QDa II Mass Detector: Selected Ion Recording (SIR) chromatogram of doubly charged components of a glycan sample. Highlighted additional components identified s a result of the extended mass range." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Waters_ACQUITY_Q_Da_II_Mass_Detector_Selected_Ion_Recording_SIR_chromatogram_of_doubly_charged_components_of_a_glycan_sample_Highlighted_additional_components_identified_s_a_result_of_the_extended_mass_range_ac0160e74d.jpg 238w," sizes="100vw" width="1347" height="883"></p><h3><strong>Unparalleled Robustness for More Qualitative and Quantitative Performance</strong></h3><h4><strong>Reduce the risk of genotoxic impurities formation in drug manufacturing</strong></h4><p data-start="0" data-end="296">Support risk assessment through routine monitoring of excipients and raw materials in drug manufacturing. Accessible, regulatory compliant-ready LC-MS solutions provide an efficient and cost-effective approach in mitigating the risk of impurity formation during the manufacture of drug product.</p><p data-start="298" data-end="768">Nitrite, a critical risk factor in N-Nitrosamine formation, presents as an impurity in pharmaceutical excipients, often in parts per million (ppm). The ACQUITY QDa II Mass Detector is a robust and reliable solution for routine analysis of nitrite in excipients. With automated resolution, tuning, and calibration during start-up, the ACQUITY QDa II Mass Detector eliminates the need for analyst intervention, ensuring seamless operation and precise results every time.</p><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Waters_ACQUITY_Q_Da_II_Mass_Detector_Over_29_days_of_rigorous_testing_it_flawlessly_executed_approximately_6000_injections_with_minimal_downtime_02a496f422.jpg" alt="Waters ACQUITY QDa II Mass Detector: Over 29 days of rigorous testing, it flawlessly executed approximately 6000 injections with minimal downtime." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Waters_ACQUITY_Q_Da_II_Mass_Detector_Over_29_days_of_rigorous_testing_it_flawlessly_executed_approximately_6000_injections_with_minimal_downtime_02a496f422.jpg 245w," sizes="100vw" width="1185" height="529"></p><p data-start="770" data-end="1012" data-is-last-node="" data-is-only-node="">Empower CDS reporting functionality offers simple reviewing of excipient nitrite concentration within a sample batch. This allows for fast and comprehensive decision making, while ensuring compliance and data integrity within your laboratory.</p><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Waters_ACQUITY_Q_Da_II_Mass_Detector_Example_of_Empower_CDS_generated_data_report_for_nitrite_content_in_a_maize_starch_sample_2f106193ee.jpg" alt="Waters ACQUITY QDa II Mass Detector: Example of Empower CDS generated data report for nitrite content in a maize starch sample." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Waters_ACQUITY_Q_Da_II_Mass_Detector_Example_of_Empower_CDS_generated_data_report_for_nitrite_content_in_a_maize_starch_sample_2f106193ee.jpg 245w," sizes="100vw" width="937" height="585"></p><h3><strong data-start="0" data-end="62">Improving Efficiency with a More Sustainable Mass Detector</strong></h3><h4><strong>Low operational costs and environmental footprint</strong></h4><p data-start="123" data-end="346"><strong>As a leading technology innovator in chromatography, mass spectrometry, and thermal analysis instruments and software, Waters shares a common mission with our customers: to leave the world a better place than we found it.</strong></p><p data-start="348" data-end="674" data-is-last-node="" data-is-only-node="">Many of our customers are actively working to address environmental sustainability in balance with the need to continually reduce operational costs. Waters has addressed these challenges by providing an instrument with low electricity consumption and BTU/hr output – lessening the need for air conditioning and lowering costs.</p><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Waters_ACQUITY_Q_Da_II_Mass_Detector_Improving_Efficiency_with_a_More_Sustainable_Mass_Detector_Low_operational_costs_and_environmental_footprint_a9e182ea1f.jpg" alt="Waters ACQUITY QDa II Mass Detector: Improving Efficiency with a More Sustainable Mass Detector. Low operational costs and environmental footprint." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Waters_ACQUITY_Q_Da_II_Mass_Detector_Improving_Efficiency_with_a_More_Sustainable_Mass_Detector_Low_operational_costs_and_environmental_footprint_a9e182ea1f.jpg 245w," sizes="100vw" width="1124" height="389"></p><p><strong>*</strong>From competitor A Specification Sheet.</p><p><strong>**</strong>From competitor B Site Preparation Checklist</p><h3><strong>Unrivaled Support</strong></h3><h3><strong>Ensure your success with world-renowned services</strong></h3><p data-start="77" data-end="464" data-is-last-node="" data-is-only-node="">Waters Service and Support helps maintain peak system performance, minimize downtime, address scientific challenges, and meet compliance requirements.&nbsp;</p><p>Waters award-winning consistent quality support, professional services, and education ensure your success wherever your laboratory is located in the world. With Waters service and support offerings<strong>*</strong> for your ACQUITY QDa II Mass Detector, you can</p><h4><strong data-start="0" data-end="30">GET UP AND RUNNING QUICKLY</strong></h4><p data-start="0" data-end="235">Get the onsite training and consultation you need to integrate the ACQUITY QDa II Mass Detector into your laboratory and assist with data migration and method transfer needs with Waters Professional Services.</p><h4><strong data-start="237" data-end="276">MEET YOUR LAB’S UNIQUE REQUIREMENTS</strong></h4><p data-start="237" data-end="460">Protect your instrument investment with the comprehensive portfolio of service plans from Waters FlexCHOICE™, which provides access to the most in-depth and up-to-date knowledge of the advanced science and technologies within Waters systems.</p><h4><strong data-start="462" data-end="487">FACILITATE COMPLIANCE</strong></h4><p data-start="462" data-end="635">Simplify the process of qualification, and record-keeping so that your business can focus on delivering products to market with Waters Compliance Services – so you can meet the regulations in your industry and in the countries where you conduct business.</p><h4><strong data-start="637" data-end="671">REDUCE TOTAL COST OF OWNERSHIP</strong></h4><p data-start="637" data-end="864">FlexCAPITAL™ <strong>**</strong> is designed to support continuous laboratory operation while satisfying the budgeting challenges of a constantly shifting landscape. Minimize total cost of ownership while optimizing instrument performance and protecting your organization from security risks associated with outdated software platforms.</p><h4><strong data-start="866" data-end="903">LEVERAGE YOUR EXISTING TECHNOLOGY</strong></h4><p data-start="866" data-end="1104" data-is-last-node="" data-is-only-node="">With the FlexUP™ Technology renewal program, you can upgrade your older single quadrupole mass spectrometry system to the ACQUITY QDa II Mass Detector -- improving performance, results, and productivity while controlling costs and mitigating compliance risk.</p><p><strong>*</strong> Availability of offerings varies by country. Contact Waters or your local Waters representative for details<br><strong>**</strong> FlexCAPITAL payment plan options and availability may vary by country. Other restrictions may apply.</p>

Wiley LC-HR-MS/MS Library of Drugs, Poisons and Their Metabolites

The updated MMHW LC-HR-MS/MS library offers 5,500+ spectra of drugs, poisons, and metabolites. A trusted resource for toxicology, doping control, and environmental analysis.

<h4><strong>From the Leader in Spectral Data</strong></h4><p>The <i><strong>Maurer, Meyer, Helfer, Weber: LC-HR-MS/MS Library of Drugs, Poisons, and Their Metabolites, 2nd Edition</strong> </i>(MMHW) is one of the most comprehensive LC-MS resources for clinical and forensic toxicologists. Developed by Prof. Hans H. Maurer and his team, the database emphasizes metabolites essential for accurate toxicological screening.</p><p>This edition contains <strong>over 5,500 high-quality spectra</strong>, covering <strong>2,300+ parent drugs/poisons</strong> and <strong>3,200+ metabolites or artifacts</strong> across 100+ classification groups. Substances are categorized by toxicological relevance, with accompanying methods in PDF format.</p><p>The data is <strong>carefully curated and reviewed</strong> by experts, ensuring reliable compound identification. Wiley collaborates with major MS vendors, supporting multiple instruments and formats for seamless workflow integration.</p><h4><strong>Applications</strong></h4><p>The MMHW library is trusted worldwide for compound analysis and verification in:</p><ul><li>Clinical and forensic toxicology</li><li>Doping control</li><li>Environmental and food chemistry</li><li>Pharmacokinetics and pathology</li></ul><h4><strong>What’s New</strong></h4><p>The 2nd edition adds <strong>550+ new datasets</strong>, including designer drugs, cannabinoids, opioids, stimulants, and more.</p><h4><strong>Specifications</strong></h4><ul><li><strong>Spectra: </strong>5,558</li><li><strong>Structures: </strong>4,270</li><li><strong>Unique Compounds:</strong> 2,763</li><li><strong>Metabolites/Artifacts:</strong> &gt;3,250</li></ul><p><strong>Available in KnowItAll and NIST formats</strong>, this library remains a critical resource for accurate, sensitive, and selective LC-HR-MS/MS screening.</p><p>Search compound coverage at <a target="_blank" rel="noopener noreferrer" href="https://spectrabase.com/"><strong>compoundsearch.com</strong></a>.</p>

Products focused on LC/MS - page 3

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