<h3><a target="_blank" rel="noopener noreferrer" href="https://www.isc2026.org/">ISC 2026</a></h3>The <a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/companies/314">International Symposium on Chromatography (ISC)</a> represents the oldest conference series on separation science. ISC symposia have been organised since 1956 in each even year. ISC is one of the premier meetings series for discussion of all modes of chromatography and separation science with a broad coverage of techniques and applications.The major focus of the symposium will be on the impact of chromatography and separation science to meet the needs of the pharmaceutical, environmental, food and health industry, as well as science and medicine. The symposium programme will reflect these themes and highlight new challenges and emerging opportunities for the science and marketing of separation and detection systems and methods.As an integral part of the scientific programme of lectures and poster sessions, an international exhibition and vendor seminars on instrumentation and services for chromatography, separation science and mass spectrometry will be organised. ISC 2026 will provide the perfect forum for attendees from academia, industry and government research institutions for scientific exchange and networking.<h3>REGISTRATION</h3><blockquote>👉<a target="_blank" rel="noopener noreferrer" href="https://www.czech-in.org/cmPortalV15/Account/Login?ReturnUrl=%2FcmPortalV15%2FPortal%2FISC26%2Fnormal">Register NOW and do not miss the deadline</a><ul><li>Early-bird registration &amp; payment deadline: May 15, 2026</li><li>Payment deadline for authors of oral presentations: May 15, 2026</li></ul></blockquote><h3>TUTORIAL LECTURES</h3><h5>Bezhan Chankvetadze</h5>Bezhan Chankvetadze is Professor for Physical Chemistry and director of the Institute of Physical and Analytical Chemistry at the Tbilisi State University in Tbilisi, Georgia.He is the Editor-in-Chief of the Journal of Pharmaceutical and Biomedical Analysis and a member of the editorial boards several international journals.Chankvetadze is the recipient of “2006 Belgian Society of Pharmaceutical Science Award of Recognition”, the joint Csaba Horvath Memorial Award of the Hungarian Separation Science Society and Connecticut Separation Science Council, USA (2017) and Nernst-Tswett Award from the European Separation Science Society (EuSSS) (2024).<img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/ISC_Bezhan_Chankvetadze_b97143b6b0.jpg" alt="ISC 2026: Bezhan Chankvetadze" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_ISC_Bezhan_Chankvetadze_b97143b6b0.jpg 156w," sizes="100vw" width="480" height="480"><h5>Martin Gilar</h5>Dr. Martin Gilar received his Ph.D. in analytical chemistry from Institute of Chemical Technology in Prague (1996). He spent postdoc years in Hybridon Inc. (1996-1998) and Northeastern University in Boston (1998) developing separation methods for antisense oligonucleotides and fraction collector for DNA molecules. Since 1998 he has worked at Waters Corp. in Milford, Massachusetts, participating in column, sample preparation and instrument research.Dr. Martin Gilar is a Research Fellow in the Separations R&amp;D group at <a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/companies/325">Waters Corporation</a>. He has 30+ years of experience in the separation sciences, including chromatography, electrophoresis, and mass spectrometry. His research interest is the analysis of biopolymers, peptides, and nucleic acids. He has published over 75 peer reviewed papers. Dr. Gilar is recipient of Jaroslav Janak 2022 medal and Chromatographic Society Jubilee medal in 2023.<img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/ISC_Martin_Gilar_3545a57ff2.jpg" alt="ISC 2026: Martin Gilar" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_ISC_Martin_Gilar_3545a57ff2.jpg 156w," sizes="100vw" width="480" height="480"><h5>Václav Kašička</h5>Václav Kašička is head of Laboratory of Electromigration Methods (www.uochb.cz/kasicka) at the <a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/companies/62">Institute of Organic Chemistry and Biochemistry of the Czech Academy of Sciences</a> since 1995. He is engaged in development of electroseparation methods and their application for analysis and characterization of (bio)molecules. He is (co)author of 200 papers in impacted journals, 20 book chapters, and 160 lectures at international symposia, with 4922 citations, h-index 39. He is associated editor of the Journal of Separation Science, and member of editorial boards of Electrophoresis, J. Chromatogr. A and other journals. He is chair of Chromatography and Electrophoresis Group of the Czech Chemical Society, and member of permanent Scientific Committees of International Symposia ITP YYYY and LACE YYYY.<img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/ISC_Vaclav_Kasicka_520987cd65.jpg" alt="ISC 2026: Václav Kašička" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_ISC_Vaclav_Kasicka_520987cd65.jpg 156w," sizes="100vw" width="480" height="480"><h5>Jörg P. Kutter</h5>Professor Dr. Jörg P. Kutter holds a B.Sc. and a Ph.D. in analytical chemistry (1995) from the University of Ulm, Germany, focusing on chromatographic and electrophoretic techniques.After graduation, he was a postdoctoral fellow at Oak Ridge National Laboratory (Oak Ridge, TN, USA) developing microchip-based analytical tools. He joined the Technical University of Denmark (DTU) in 1998, and in 2006, he was appointed professor in experimental lab-on-a-chip systems.Since September 2013, Dr. Kutter is the Chair of Analytical Biosciences at the Dept. of Pharmacy at the University of Copenhagen. His research interests focus on the development of miniaturized separation and sample preparation methods.<img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/ISC_Joerg_P_Kutter_15b28eb930.jpg" alt="ISC 2026: Jörg P. Kutter" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_ISC_Joerg_P_Kutter_15b28eb930.jpg 156w," sizes="100vw" width="480" height="480"><h5>John A. McLean</h5>John A. McLean is Dean of Research Graduate Education, Stevenson Professor of Chemistry, and Director of the Center for Innovative Technologies at Vanderbilt University. McLean and colleagues have focused on the conceptualization, design, and construction of ion mobility and structural mass spectrometers, specifically targeting complex samples in systems, synthetic, and chemical biology. His group applies these strategies to forefront translational research areas in drug discovery, personalized medicine, and ‘human-on-chip’ synthetic biology platforms. He is an elected Fellow of NAI, AAAS, RCS, and ASMS and has published over 200 manuscripts and received over 30 patents in these and allied areas.<img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/ISC_John_A_Mc_Lean_62fe6879ae.jpg" alt="ISC 2026: John A. McLean" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_ISC_John_A_Mc_Lean_62fe6879ae.jpg 156w," sizes="100vw" width="480" height="480"><h5>Peter Schoenmakers</h5>Peter Schoenmakers has been a full-time professor in Analytical Chemistry at the University of Amsterdam since 2002, investigating many aspects of one- and two-dimensional chromatography, with a special interest in macromolecules and computer-assisted method development.Together with Bob Pirok, Peter Schoenmakers has recently completed a new textbook on Analytical Separation Science. He has received a number of international awards, most recently the ACS Award for Chromatography (2022) and the LCGC lifetime-achievement award (2023). He received royal recognition when he became Knight in the Order of the Dutch Lion in 2022.<img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/ISC_Peter_Schoenmakers_6962648388.jpg" alt="ISC 2026: Peter Schoenmakers" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_ISC_Peter_Schoenmakers_6962648388.jpg 156w," sizes="100vw" width="185" height="185"><h5>František Švec</h5>Frantisek Svec is working at the Faculty of Pharmacy at Charles University in Hradec Kralove, Czech Republic. He has authored over 560 publications, which have been cited more than 25,000 times, giving him an h-index of around 100. F. Svec is best known for his research on monoliths and their use in many different applications. Currently, he is focusing on developing novel formats of sorbents for sample preparation.<img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/ISC_Frantisek_Svec_9413f4a131.jpg" alt="ISC 2026: František Švec" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_ISC_Frantisek_Svec_9413f4a131.jpg 156w," sizes="100vw" width="480" height="480">

Tutorial Lectures at ISC 2026

Don't miss this unique opportunity to stand out at the upcoming HTC-19 conference by winning the HTC Tube competition! Deadline for submissions is coming up soon on Friday 15 May! The winners get 750 Euro (first prize), 500 Euro (second prize) and 250 Euro (third prize). That's a lot of Belgian Beer!<h4>Criteria for evaluation</h4><ul><li>We invite scientists to create short videos (up to 3 minutes) that showcase how your chromatography work impacts the world.&nbsp;</li><li>No need for professional editing, just grab your smartphone and start filming.&nbsp;</li><li>Best video will be selected via electronic audience poll, based on scientific content, entertainment value, creativity and technical skills.</li></ul><h4>Who can participate?</h4><ul><li>Eligibility: Scientists (academic or industrial) of any age involved in research projects related to the liquid chromatography workflow (before, during, or after).</li><li>Submissions: Both individual and group submissions are welcome. The video must be based on the presenter’s own findings or work.</li></ul><h4>Deadline and how to submit?</h4><ul><li>Deadline: Friday 15 May 2026</li><li>Please send your submission to info@htc-19.com</li></ul>

HTC Tube Competition at the 19th International Symposium on Hyphenated Techniques in Chromatography and Separation Technology (HTC-19)

<h4>Advancing Glycoproteomics with Flexible LC-MS at the University of Washington</h4>Understanding how cells communicate, migrate, and interact with the immune system often comes down to what is happening on their surface. For Nick Riley, Assistant Professor at the University of Washington, that surface is defined by glycans, complex sugar structures attached to proteins that play a critical role in health and disease.<img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Thermo_Fisher_Scientific_What_Happens_When_Glycoproteomics_Meets_Real_Time_LC_MS_Decision_Making_7f77f81741.jpeg" alt="Thermo Fisher Scientific: What Happens When Glycoproteomics Meets Real-Time LC-MS Decision Making?: Nick Riley, Assistant Professor Department of Chemistry, University of Washington, head of the Riley Group." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Thermo_Fisher_Scientific_What_Happens_When_Glycoproteomics_Meets_Real_Time_LC_MS_Decision_Making_7f77f81741.jpeg 208w," sizes="100vw" width="1024" height="768">The <a target="_blank" rel="noopener noreferrer" href="https://www.riley-research.com/">Riley Group</a> operates at the intersection of glycobiology and mass spectrometry, developing new analytical methods to answer biological questions that were previously out of reach. Based out of one of the largest mass spectrometry hubs in the United States, the lab relies on a high-performance LC-MS workflow built around the <a target="_blank" rel="noopener noreferrer" href="https://www.thermofisher.com/content/lifetech/global/en/home/industrial/mass-spectrometry/liquid-chromatography-mass-spectrometry-lc-ms/lc-ms-systems/orbitrap-lc-ms/orbitrap-tribrid-mass-spectrometers/orbitrap-ascend-tribrid-mass-spectrometer/systems/orbitrap-ascend-multiomics-tribrid-mass-spectrometer.html">Thermo Scientific Orbitrap Ascend MultiOmics Tribrid Mass Spectrometer</a> coupled with a <a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/products/398">Thermo Scientific Vanquish Neo UHPLC System</a>.Together, these platforms deliver flexible, reproducible separations and enable the integration of ETD, HCD, PTCR, and high-mass analysis within a single experiment. This flexibility is critical when working with limited or precious samples such as enriched cell surface glycoproteins, providing the sensitivity and robustness needed to characterize highly complex glycopeptides and intact glycoproteins.<h3>From forensics to glycoproteomics</h3>Riley’s scientific path began in undergraduate chemistry and psychology, where an early interest in forensics led him to mass spectrometry. Rather than focusing on applications, he became interested in the instruments themselves.That fascination took him to the University of Wisconsin-Madison, where he trained in Josh Coon’s lab and worked extensively on electron transfer dissociation. While developing new fragmentation methods, Riley encountered glycopeptides almost by accident and quickly realized their complexity and importance.A postdoctoral position in Carolyn Bertozzi’s lab at Stanford deepened his exposure to glycobiology and reinforced his interest in building tools that allow scientists to better interrogate biological systems. That philosophy now defines his research program at the University of Washington.<h3>Why flexibility matters in glycoproteomics</h3>Glycoproteomics presents unique analytical challenges. Glycans increase molecular heterogeneity, complicate ionization, and require multiple fragmentation strategies to fully characterize.“There’s no single method that can answer all of our questions,” Riley explains. “We need flexibility at every level.”For the Riley Group, that flexibility comes from advanced LC-MS platforms, particularly Tribrid instruments like the Orbitrap Ascend Mass Spectrometer. These systems allow his team to combine ETD, HCD, PTCR, and high-mass analysis within a single workflow.That versatility is essential when working with limited samples, especially in experiments involving cell surface glycoproteins or double enrichment strategies.<h3>Turning technology into biological insight</h3>One recent study highlights how instrumentation directly enables discovery. Riley’s team investigated how glycosylation affects protein corona formation using SEER nanoparticles. The workflow required two enrichment steps, leaving very little material for analysis.Using the Orbitrap Ascend Mass Spectrometer’s real-time library search capabilities, the team developed Autonomous Dissociation Type Selection, a method that uses real-time decision making to determine the optimal fragmentation method during acquisition. This approach allowed the team to extract comprehensive information from a single LC-MS injection, preserving sample while accelerating discovery.“That kind of experiment simply wouldn’t be possible without real-time decision making on the instrument,” says Riley.<img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Thermo_Fisher_Scientific_What_Happens_When_Glycoproteomics_Meets_Real_Time_LC_MS_Decision_Making_2_f5c7de51c3.jpeg" alt="Thermo Fisher Scientific: What Happens When Glycoproteomics Meets Real-Time LC-MS Decision Making?: Kayla Markuson, B.S., is one of the grad students in the Riley Group. She was recipient of the 2025 Thermo Scientific Quantitative Proteomics Research Grant Program." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Thermo_Fisher_Scientific_What_Happens_When_Glycoproteomics_Meets_Real_Time_LC_MS_Decision_Making_2_f5c7de51c3.jpeg 208w," sizes="100vw" width="1024" height="768"><h3>Lowering barriers, training scientists</h3>Beyond enabling specific experiments, Riley sees advanced instrumentation as a way to democratize complex methodologies. Techniques like ETD, once difficult to reproduce, are now robust and accessible.That shift has a direct impact on training. Students in Riley’s lab are encouraged to design experiments around unanswered biological questions rather than technical constraints.“When the instrument isn’t the bottleneck, creativity becomes the skill you’re really teaching,” he notes. “That’s how you prepare scientists for the future.”<h3>Looking ahead</h3>As instrumentation continues to evolve, Riley believes the next breakthroughs will come from increased sensitivity, richer fragmentation strategies, and smarter data analysis. The ultimate goal is not just better measurements, but deeper biological understanding.“Glycoproteomics demands multiple analytical strategies,” Riley explains. “We need to move seamlessly between different fragmentation methods, mass ranges, and separation conditions. The Ascend paired with the Vanquish Neo gives us that freedom.”“Technology should expand the questions we can ask,” he says. “That’s when real discovery happens.”&nbsp;<img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Thermo_Fisher_Scientific_What_Happens_When_Glycoproteomics_Meets_Real_Time_LC_MS_Decision_Making_3_ced9ad52e2.jpeg" alt="Thermo Fisher Scientific: What Happens When Glycoproteomics Meets Real-Time LC-MS Decision Making?: Lab members pictured left to right in front of Riley Group’s Thermo Scientific Orbitrap Ascend MultiOmics Tribrid Mass Spectrometer coupled with a Thermo Scientific Vanquish Neo UHPLC System: Nick Riley, Emmajay Sutherland, Jacob Russell, Tim Veth, Kayla Markuson." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Thermo_Fisher_Scientific_What_Happens_When_Glycoproteomics_Meets_Real_Time_LC_MS_Decision_Making_3_ced9ad52e2.jpeg 208w," sizes="100vw" width="768" height="576"><blockquote>Visit us on&nbsp;<a target="_blank" rel="noopener noreferrer" href="https://www.linkedin.com/showcase/72003132/admin/dashboard/">LinkedIn:</a>&nbsp;#Glycoproteomics #MassSpectrometry #LCMS</blockquote>

What Happens When Glycoproteomics Meets Real-Time LC-MS Decision Making?

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Thermo Fisher Scientific

The International Symposium on Chromatography (ISC) represents the oldest conference series focussing on separation science. ISC symposia have been organised since 1956 in each even year. ISC is internationally recognised as one of the premier meeting series for discussion of all modes of chromatography and separation science with a broad coverage of techniques and applications.

International Symposium on Chromatography - (ISC)

The HTC symposium series started in 1990 and is the ideal meeting place for scientists and practitioners from academia and industry and vendors to discuss and gather new insights in emerging technologies, instrumentation, workflows, and applications. Organized under the auspices of the Vrije Universiteit Brussel (VUB), the Catholic University of Leuven (KU Leuven), Ghent University (UGhent), and the Separation Science Group of the Royal Society of Chemistry (RSC), HTC-19 is expected to gather over 300 participants.

International Symposium on Hyphenated Techniques in Chromatography and Separation Technology

Agilent Technologies

PerkinElmer

SelectScience

Waters Corporation

Starting with defining the basics terms used to describe chromatographic separations and finishing with a strategy for running and interpreting a scouting gradient, in this talk we will look at the basic essentials needed to efficiently develop an HPLC method.
<blockquote>
Presenter: Paul Altiero (Application Engineer, Agilent Technologies, Inc.)
</blockquote>
<blockquote>
Paul Altiero is an Applications Engineer located at the Agilent Little Falls site in Wilmington, DE. Prior to coming to Agilent in 2009, Paul worked in the pharmaceutical industry performing LCMS bioanalysis supporting Drug Discovery DMPK. While at Agilent, Paul has been a field service engineer for LCMS as well as the integrating program manager for the RapidFire high throughput LCMS system. In his current role Paul’s responsibilities include technical support and application assistance for LCMS consumables.
</blockquote>

Agilent Technologies: Essentials of Good HPLC Method Development

Essentials of Good HPLC Method Development

In this talk we will look at the basic essentials needed to efficiently develop an HPLC method.

In this webinar we will discuss some important considerations to help maximize resolution when developing an HPLC method.
<ul>
<li>What should I consider in selecting a column for my separation goals?</li>
<li>What method parameters can I adjust to reduce the chance of method variability?</li>
<li>What role does my HPLC instrument play in optimizing a method?</li>
</ul>
Maximizing resolution with a robust and rugged method requires a strategy that addresses both chemistry and instrumentation.
<blockquote>
Presenter: Mark Powell (Application Engineer, Agilent Technologies, Inc.)
</blockquote>
<blockquote>
Mark Powell is an Applications Engineer located at Agilent’s Little Falls site in Wilmington, DE. Before joining Agilent in 2011, Mark worked in the pharmaceutical industry synthesizing, purifying and analyzing drug candidates. He provides applications assistance and technical support for Agilent’s HPLC columns and consumables.
</blockquote>

Agilent Technologies: Tips to Help Maximize Resolution

Tips to Help Maximize Resolution

In this webinar we will discuss some important considerations to help maximize resolution when developing an HPLC method.

A calibration curve or regression is always plotted with the instrument responses on the dependent vertical (y -) axis and the standard concentrations on the horizontal (x -) axis. As a linearity of calibration regression minimizes random errors of the instrument, we aim to find the best straight line to pass through these calibration points.
<ul>
<li>What are the estimated errors and confidence limits we can estimate for the slope (or gradient) and the y-intercept of the line?
</li>
<li>What is the uncertainty of the analyte concentration found on the calibration curve?
</li>
<li>What are the least concentration of the measurand can be detected with, say 95% confidence?
</li>
<li>In other words, what is the limit of detection (LOD) of this method?
</li>
</ul>
This one-day training program is designed to answer all the above questions and others related to the subjects of linear regression of instrument calibration and detection limits including IDL, MDL and LOQ.
<h3>Training Program (8:00 - 10:30 CEST)</h3>
<ul>
<li>Introduction</li>
<li>Different meanings for correlation and regression</li>
<li>Assumptions of linear regression and correlation</li>
<li>Residual analysis – what and how</li>
<li>Uncertainty estimation of linear calibration regression</li>
<li>Must your linear regression always pass through its origin (0,0)?</li>
<li>The most controversial subject in analytical chemistry – Limit of Detection LOD</li>
<li>Different definitions for LOD and its basic concepts</li>
<li>Differences between IDL, MDL, LOQ</li>
<li>Determining LOD by linear calibration model</li>
</ul>
<h5>Who should attend:</h5>
This course is designed for those who want to know more about the sampling procedures and how it affects the validity of their test results, including:
<ul>
<li>Laboratory analysts</li>
<li>Laboratory QA/QC personnel</li>
<li>Lab directors / managers</li>
<li>Personnel who carry out tasks such as method validation and uncertainty estimation</li>
</ul>
<h5>Pre-requisites</h5>
The participants are assumed to have had some basic statistical knowledge such as the calculation of mean, standard deviation, coefficient of variation, relative standard deviation, etc.
Participants are to have a laptop with Microsoft Excel installed and have some very basic knowledge of how to use the program.
<blockquote>
Presenter: Yeoh Guan Huah
</blockquote>
<blockquote>
Mr Yeoh Guan Huah has B.Sc. (Hons) and M.Sc. degrees in Chemistry and was former Managing Director of ALS Technichem Singapore (1978-2006), former East Asia Regional Manager of ALS Group of Laboratories in Hong Kong and China (2006-2016). He is now the senior partner of GLP Consulting, Singapore.
</blockquote>
<blockquote>
Currently Mr Yeoh is an appointed External Lead Assessor and Technical Assessor of SAC-SINGLAS till 31 March 2025 and had represented DSM/SIRIM Malaysia as a active member of ISO Technical Committee TC 69 (Application of Statistical Methods) and Convenor of AHG1 from 2005-2016.
</blockquote>

Agilent Technologies: Uncertainty of Linear Calibration Curve & Detection Limit

Uncertainty of Linear Calibration Curve & Detection Limit (Part 1)

We will answer all questions related to the subjects of linear regression of instrument calibration and detection limits including IDL, MDL and LOQ.

Uncertainty of Linear Calibration Curve & Detection Limit

In this webinar, join our low flow LC expert who will review micro and nano LC fundamentals, and present a selective approach to trap and column strategies to increase method performance. They will provide chromatographic examples on forward and backward trap elute strategies, trap selectivity comparisons, and micro and nano LC column selectivities, and will explain how to find the best selectivity strategy for your application.
<h5>Key learning objectives</h5>
<ul>
<li>Understand the current state of micro and nano LC and hardware advances</li>
<li>Understand trap and elute – forward and backward comparison</li>
<li>Discover trap and column selectivity strategies</li>
<li>Learn how to find the best selectivity for your application</li>
</ul>
<h5>Who should attend?</h5>
<ul>
<li>Any scientist who is interested in improving and expanding their micro and Nano LC performance and would like to increase their knowledge of current trap and column strategies and hardware advancements</li>
</ul>
<blockquote>
Presenter: Roxana Eggleston-Rangel (Senior Applications Scientist, Phenomenex)
</blockquote>
<blockquote>
Presenter: Dora Wells (SelectScience)
</blockquote>

SelectScience: What You Need to Know: Micro and Nano Low Flow LC Essentials

What You Need to Know: Micro and Nano Low Flow LC Essentials

In this webinar we will review micro and nano LC fundamentals, and present a selective approach to trap and column strategies to increase method performance.

<h3>Two-dimensional liquid chromatography (2D-LC): A tutorial focusing on best practice</h3>
In recent years, 2D-LC has evolved from a niche technology to one that is in demand, especially when it comes to the analysis of complex samples, or hidden or co-eluting compounds. Whether you are starting out with 2D-LC or want to refresh your knowledge, this two-part webinar series will give you a comprehensive introduction to the technique, including best practices.
<h4>Session 1: Key concepts of 2D-LC</h4>
Learn about the four modes of 2D-LC: heart-cutting, multiple heart-cutting, selective and full comprehensive, and find out how they relate conceptually. Plus, get advice on how to choose complementary separations for the first and second dimension (RP, IEX, HILIC, etc.), how to optimize cycle time, and how to maximize second dimension sensitivity.
<h5>Who should attend?</h5>
New and experienced 2D-LC users, scientists, and lab managers or technical decision makers in pharma, biopharma, food, energy &amp; chemicals, environmental, material, forensics, and life science research
<blockquote>
Presenter: Dr. Dwight Stoll (Professor of Chemistry, Gustavus Adolphus College. St. Peter, MN, USA)
</blockquote>
<blockquote>
Dr. Dwight Stoll completed his undergraduate work at Minnesota State University, Mankato, in plant biology and biochemistry. Upon graduation in 1999, he took a job in industry as a research technician with ZirChrom Separations, Inc. For his graduate work, he studied with Professor Peter Carr in the Chemistry Department at the University of Minnesota from 2001–2007, where he worked on the development of fast, comprehensive two-dimensional liquid chromatography.
</blockquote>
<blockquote>
In the fall of 2008, Stoll was hired as an Assistant Professor in the Chemistry Department at Gustavus Adolphus College, where he mainly teaches quantitative and instrumental analysis courses. In 2014, he was promoted to Associate Professor, and in 2018, he was promoted to Professor. Currently, he is co-chair of the Chemistry Department. Active research projects in his laboratory touch upon most aspects of multi-dimensional separation methodologies, including optimization strategies, characterization of selectivity in reversed-phase HPLC, instrument development, and applications in biopharmaceutical analysis.
</blockquote>
<blockquote>
Stoll is the author or co-author of more than 62 peer-reviewed publications and three book chapters in the area of separation science, is a named co-inventor on four granted patents, and has instructed numerous short courses in two-dimensional liquid chromatography. In 2011, he was the recipient of LCGC’s Emerging Leader in Chromatography Award. In 2014, he added to The Analytical Scientist’s list of ‘Top 40 Under 40’ analytical scientists. In 2017, he received the Georges Guiochon Faculty Fellowship and an Agilent Technologies Thought Leader Award, which will support research in his laboratory on the development of 2D-LC methodologies for biopharmaceutical analysis.
</blockquote>

Agilent Technologies: Two-dimensional liquid chromatography (2D-LC): A tutorial focusing on best practice

Key concepts of 2D-LC

Learn about the four modes of 2D-LC: heart-cutting, multiple heart-cutting, selective and full comprehensive, and find out how they relate conceptually.

High Performance Liquid Chromatography (HPLC) is an important technique used in Analytical Laboratories for the identification and quantitation of different analytes/compounds in samples. It is the most versatile and dependable chromatographic technique for quality control.
In this seminar, we will cover the complete LC workflow, starting from mobile phase to detection, the different parts involved in this flow path and the different consumables associated with each module of LC system and their importance in acquiring chromatographic results.
<h5>Please join us in this webinar to learn about:</h5>
<ul>
<li>Introduction to LC workflow</li>
<li>Sample flow path and the respective modules involved from Mobile phase to Detection.</li>
<li>Importance of small consumable parts of each module for smooth running of LC system.</li>
<li>How to enhance productivity with new innovative technology products in day-to-day chromatography analysis.</li>
</ul>
<h5>Who Should Attend:</h5>
<ul>
<li>Users of Liquid chromatography in Pharma, Academia &amp; Applied Markets</li>
<li>Analytical scientists, chemists, and lab managers trying to enhance their lab productivity.</li>
</ul>
<blockquote>
Presenter: Dr. Ramaprasad Ganni (Technical Support Specialist, Agilent Technologies)
</blockquote>
<blockquote>
Ramaprasad Ganni has 21 years of experience in the field of Chromatography and Column chemistries. Working with Agilent technologies India, since 2008 in Chemistries and Supplies division. Previously Worked for Elsevier GmbH, and Millipore India Pvt, Ltd. Responsible for Workflow solutions development, providing technical support, and trainings. Experienced in Sample prep, HPLC, GC, Mass spec and GPC Chromatography technologies .
</blockquote>

Agilent Technologies: Mobile Phase to Detector - Complete Workflow

Mobile Phase to Detector - Complete Workflow

In this seminar, we will cover the complete LC workflow, starting from mobile phase to detection

A UV-VIS spectrometer is a powerful and versatile tool for material analysis. It is capable of providing very quick information of sample composition and concentration for quality control in addition to indicating how samples change versus time or temperature.
In this webinar, we will go over what is happening when we take a UV-VIS measurement, discuss how measurement parameters can improve our data collection, and look at applications like concentration and kinetics.
<blockquote>
Presenter: Scott Melis, PhD (Application Scientist, Agilent Technologies, Inc.)
</blockquote>
<blockquote>
Scott Melis has a PHD in Physics from Georgetown University in Washington, DC and started with Agilent in 2021 as a Molecular Spectroscopy Application Scientist. In his graduate work, Scott studied nanoparticle formation and growth for a variety of applications. Projects that he worked with include developing processes to deposit and characterize semiconducting molecular nano-cocrystals for use in optoelectronic devices as well as controlling polymer nanoparticle size through rapid mixing conditions.
</blockquote>

Agilent Technologies: Understanding Your UV-Vis Measurements

Understanding Your UV-Vis Measurements

What is happening when we take a UV-VIS measurement, how measurement parameters can improve our data collection, and look at applications.

In this educational webinar series, we will go back to the basics of chromatography, mass spectrometry, data analysis and troubleshooting. Understanding the importance of the basics will enable you to be more effective in your laboratory. During each session we will cover a specific aspect of your LC or LC/MS system:
<ul>
<li>The theory of chromatography and requirements for great separations</li>
<li>The basics of your Liquid Chromatograph (LC): Pumps, injectors, and detectors</li>
<li>An Introduction to mass spectrometry (MS) systems</li>
<li>Considerations with System Suitability</li>
<li>Troubleshooting your LC and MS systems</li>
</ul>
<h3>**PART I | CHEMISTRY **</h3>
<ul>
<li>Theory of Chromatography</li>
<li>Creating Great Separation Methods</li>
</ul>
<blockquote>
Presenter: Chris Collins, Ph.D. (Chemistry Account Manager, Waters Corporation)
</blockquote>
<blockquote>
Chris Collins joined Waters in 2018 as a Chemistry Account Manager covering Eastern North Carolina, providing support to customers on all chemistry and consumable products. He has over twenty years’ experience in the pharmaceutical industry with a focus on LC/MS Method Development.
</blockquote>
<blockquote>
HOST: Kieron Faherty, Ph.D. (Separations and Mass Spectrometry Field Marketing Manager, Waters Corporation)
</blockquote>
<blockquote>
Kieron Faherty is the Separations and Mass Spectrometry Field Marketing Manager for the Americas at Waters Corporation. He received his B.Sc. (Hons) in Chemistry from the University of Reading (Reading, Berkshire, UK) and his doctorate in Chemistry from the University of Massachusetts (Amherst, MA). He has held several field-based positions at Waters, most recently as a Field Applications Specialist focused on biopharmaceutical applications using Time-of-Flight mass spectrometry instrumentation.
</blockquote>
<blockquote>
HOST: Alexandra (Allie) Ahern (Informatics Product Marketing Manager, Americas Field Marketing, Waters Corporation)
</blockquote>
<blockquote>
Allie Ahern has been with Waters for 10 years and is the Informatics Product Marketing Manager within the Americas Field Marketing Organization. Prior to Allie’s current role, she has held other roles at Waters including Service Marketing Product Manager, Implementation Project Manager, and Field Service Engineer.
</blockquote>

Waters Corporation: Fall Back to Basics - Part 1: Chemistry

Fall Back to Basics - Part 1: Chemistry

PART 1 topics: Theory of Chromatography. Creating Great Separation Methods.

Fall Back to Basics - Part 1: Chromatography chemistry 

This presentation is the first in a series that will introduce you to the most widely used analytical technique in the world – high performance liquid chromatography (HPLC). HPLC is a separation technique that allows you to separate, identify and quantitate the components in a sample. The objective of this session is to provide you with an entry level understanding of this technique. Topics to be discussed include chromatographic theory, the different modes of HPLC, a generic look at HPLC instrumentation, and where HPLC is used.
In later sessions in this series some of the items discussed under these topics (i.e., columns, detectors and troubleshooting) will be discussed in greater detail.
<blockquote>
Presenter: Pamela A. Perrone (Senior Principal LC Technical Education Specialist, Perkinelmer)
</blockquote>
<blockquote>
Pam has been with PerkinElmer for almost 39 years. She had the opportunity to work as a summer intern and was offered full-time employment after completing her Ph.D. in Analytical Chemistry at the University of Rhode Island. Currently Pam is in the role she finds most fulfilling and challenging which is as the global support and trainer for both service and customers for LC and chromatography data handling. To help someone learn and be successful in their job is very rewarding.
</blockquote>
<blockquote>
Pam has a passion for three things (besides LC, of course!): her family, the UConn women’s basketball team and Broadway. She believes there is a Broadway song that fits almost every occasion.
</blockquote>

PerkinElmer: Liquid Chromatography 2022 Learning Series

Introduction to HPLC

This presentation is the first in a series that will introduce you to the most widely used analytical technique in the world – HPLC.

One of the key features in developing a HPLC chromatographic method is the selection HPLC column. It is the focus of this presentation to review the key factors that go into HPLC column selection. These factors include column length, column diameter, particle size of the column packing media, pore size of the column packing media, column type and stationary phase of the packing media.
We explain how column selection can be a match in developing an optimized separation of analyte classes and ultimately how this selection can help ensure a robust separation method. We show how practical compromises can be made in selecting the best column for required separation.
<blockquote>
Presenter: Matthew Przybyciel, Ph.D. (Research and Development Leader, PerkinElmer)
</blockquote>
<blockquote>
Matthew has a PhD in Analytical Chemistry from the University of South Carolina. The focus of his research was the development of new stationary phases for capillary gas chromatography. In the late 1990’s he worked in ES Industries focusing its efforts exclusively on chromatography column products for HPLC and SFC where Matt was Vice President and Technical Director for chromatography products. He has successfully developed and commercialized a number of chromatographic stationary phases and columns for high performance chromatographic separations for HPLC and SFC.
</blockquote>
<blockquote>
In 2021, ES Industries became part of PerkinElmer, Inc. and Matthew now is product development leader at PerkinElmer. As part of his work, he has also published a number of articles on the development and application of new stationary phases for GC, HPLC and SFC.
</blockquote>

Introduction to LC Column Selection

One of the key features in developing a HPLC chromatographic method is the selection HPLC column.

LCMS webinars focusing on Academy/Principles - page 26