Microplastics and Nanomaterials: Understanding Emerging Contaminants in Environmental Testing

🎤Matthew BolinoHow do the hidden carbohydrate structures on your favorite protein powders shape the gut microbiome? &nbsp;In this episode of Concentrating on Chromatography, Matthew Bolino, M.S., from the University of Nevada, Reno, breaks down his latest research on N‑glycans from common dietary proteins (whey, egg white, soy, and pea) and how their structural diversity influences microbial fermentation and short‑chain fatty acid production.Bolino explains what N‑glycans are, why they behave like fiber in the gut, and how his team isolates and characterizes them using ethanol washes, enzymatic release (PNGase F and gut‑derived endoglycosidases), and advanced MALDI‑TOF and HILIC‑QTOF workflows. &nbsp;He also discusses his 2025 work comparing synthetic versus bovine whey N‑glycomes and mapping N‑glycan profiles across dietary protein sources, revealing how glycan architecture can reshape community diversity in in vitro fecal fermentations.Geared toward undergraduate and early‑career analytical chemists, this conversation dives into practical mass spec trade‑offs (MALDI vs QTOF vs LC/GC), real‑world troubleshooting in glycomics labs, and how microbiome‑targeted therapeutics and “symbiotic” designs may emerge from pairing specific microbes with preferred glycan structures. &nbsp;Bolino closes with career advice on building biomolecular analysis skills, understanding instrumentation fundamentals, and entering the rapidly growing field of glycomics and microbiome research.<h3>Video Transcription</h3><h4>Introduction</h4>Glycomics has emerged as a rapidly developing field within biomolecular science, expanding our understanding of post-translational modifications beyond well-known processes such as phosphorylation. Among these modifications, protein glycosylation—the covalent attachment of carbohydrate structures to proteins—plays a critical role in biological function, molecular recognition, and cellular communication.This discussion focuses on N-glycans, a class of structurally complex carbohydrates linked to proteins, and their occurrence in common dietary protein sources such as whey, egg white, soy, and pea protein. Particular emphasis is placed on their structural diversity, analytical workflows, and emerging relevance in gut microbiome research.<h4>Fundamentals of N-Glycans</h4>N-glycans are oligosaccharide structures covalently attached to proteins via nitrogen atoms, typically at asparagine residues. They represent one of the two major classes of protein glycosylation, alongside O-glycans.These glycans are characterized by:<ul><li>High structural diversity, arising from variations in monosaccharide composition and linkage patterns</li><li>Large size, typically comprising 7–15 monosaccharide units</li><li>Complex branching architectures, leading to a vast number of possible configurations</li></ul>Common monosaccharide components include:<ul><li>Mannose</li><li>Galactose</li><li>N-acetylglucosamine</li><li>N-acetylgalactosamine</li><li>Sialic acids</li></ul>Unlike free carbohydrates present in foods (e.g., glucose, lactose), N-glycans are protein-bound, which significantly influences their biological behavior.<h4>Biological Function and Dietary Relevance</h4>N-glycans play multiple roles in biological systems, including:<ul><li>Modulation of protein stability</li><li>Regulation of protein trafficking and localization</li><li>Facilitation of cell–cell communication and immune recognition</li></ul>They are particularly abundant on membrane and surface proteins, where they contribute to molecular signaling processes.From a dietary perspective, N-glycans exhibit unique behavior. Due to their size and protein-bound nature, they are not readily digested in the upper gastrointestinal tract. Instead, they can reach the colon, where they may function analogously to dietary fiber, interacting with the gut microbiota.<h4>Research Motivation</h4>The investigation of N-glycans in dietary proteins was initially driven by interest in protein nutrition and supplementation, particularly in the context of muscle growth and protein quality. However, this focus expanded upon recognition of the importance of glycosylation in biological systems.The field of glycomics offers:<ul><li>Extensive structural variability, enabling diverse biological functions</li><li>Relevance in disease mechanisms, including cancer, where glycosylation patterns can influence immune evasion</li><li>Opportunities to explore diet–microbiome interactions</li></ul>This combination of nutritional, biochemical, and biomedical relevance makes N-glycan research particularly compelling.<h4>Experimental Workflow for N-Glycan Analysis</h4>The analysis of N-glycans requires a multi-step workflow due to their covalent attachment to proteins.<h5>1. Protein Purification</h5>Dietary protein samples often contain:<ul><li>Free sugars</li><li>Lipids</li><li>Other matrix components</li></ul>To isolate glycoproteins:<ul><li>Cold ethanol precipitation is used to remove free carbohydrates</li><li>Multiple washing steps ensure removal of residual sugars</li><li>A phenol–sulfuric acid assay is applied to confirm the absence of free sugars</li></ul><h5>2. Enzymatic Release of N-Glycans</h5>N-glycans are enzymatically cleaved from proteins using:<ul><li>PNGase F (Peptide-N-Glycosidase F)</li></ul>This enzyme:<ul><li>Specifically cleaves the glycan from the protein backbone</li><li>Preserves the intact glycan structure</li></ul>Alternative approaches using gut-derived enzymes are being explored to improve accessibility and yield.<h5>3. Glycan Purification and Labeling</h5>Following release:<ul><li>Glycans are purified from the protein matrix</li><li>Chemical labeling is performed (e.g., 2-AA or procainamide tagging)</li></ul>Labeling enhances:<ul><li>Detection sensitivity</li><li>Chromatographic behavior</li></ul><h5>4. Analytical Techniques</h5>Two primary analytical platforms are employed:<ul><li>MALDI-TOF MS<ul><li>High sensitivity</li><li>Broad structural coverage</li></ul></li><li>HILIC-LC-MS<ul><li>Improved reproducibility</li><li>Enhanced chromatographic separation</li></ul></li></ul>These approaches provide semi-quantitative data, where signal intensity reflects relative abundance rather than absolute concentration.<h4>Analytical Challenges</h4>Several challenges are associated with N-glycan analysis:<h5>Low Abundance</h5><ul><li>Dietary proteins typically contain only 1–2% glycan content by weight</li><li>This limits yield and complicates downstream applications</li></ul><h5>Sample Preparation Complexity</h5><ul><li>Extensive purification is required to eliminate free sugars</li><li>Contamination risks must be carefully managed</li></ul><h5>Instrumentation Trade-Offs</h5><ul><li>Different analytical platforms offer complementary advantages</li><li>No single technique provides complete structural and quantitative information</li></ul><h4>Differences Between Plant and Animal N-Glycans</h4>Comparative analysis reveals distinct differences in glycan composition:<h5>Plant-Derived Proteins (e.g., soy, pea)</h5><ul><li>Predominantly high-mannose structures</li><li>Lower monosaccharide diversity</li><li>Simpler glycan architectures</li></ul><h5>Animal-Derived Proteins (e.g., whey, egg white)</h5><ul><li>Greater structural complexity</li><li>Inclusion of diverse monosaccharides (e.g., sialic acids)</li><li>More elaborate branching patterns</li></ul>These differences have important implications for biological function and microbial interactions.<h4>Implications for Gut Microbiome Research</h4>N-glycans represent a potential class of prebiotic substrates that can influence gut microbiota composition and activity.Key findings suggest:<ul><li>Structural diversity of glycans affects microbial utilization patterns</li><li>More complex glycans may promote greater microbiome diversity</li><li>Specific glycan structures may selectively stimulate beneficial microbes</li></ul>This has led to growing interest in:<ul><li>Synbiotic strategies, combining microbes with preferred substrates</li><li>Tailored dietary interventions to enhance metabolic outputs, such as short-chain fatty acids</li></ul><h4>Methodological Considerations</h4>A critical insight from this work is the importance of understanding analytical trade-offs:<ul><li>MALDI-TOF MS provides broader detection of glycan structures</li><li>LC-MS approaches offer greater reproducibility</li></ul>No single technique is universally optimal; instead, method selection should be guided by the specific research question.<h4>Conclusion</h4>N-glycans in dietary proteins represent a complex and biologically significant class of biomolecules with implications spanning nutrition, microbiology, and disease research. Advances in analytical methodologies continue to improve our ability to characterize these structures, despite challenges related to low abundance and structural diversity.A deeper understanding of N-glycan composition and function may enable the development of targeted nutritional strategies and microbiome-based therapeutics, highlighting the growing importance of glycomics in modern life sciences.<blockquote>This text has been automatically transcribed from a video presentation using AI technology. It may contain inaccuracies and is not guaranteed to be 100% correct.</blockquote><blockquote><h4>Concentrating on Chromatography Podcast</h4>Dive into the frontiers of chromatography, mass spectrometry, and sample preparation with host David Oliva. Each episode features candid conversations with leading researchers, industry innovators, and passionate scientists who are shaping the future of analytical chemistry. From decoding PFAS detection challenges to exploring the latest in AI-assisted liquid chromatography, this show uncovers practical workflows, sustainability breakthroughs, and the real-world impact of separation science. Whether you’re a chromatographer, lab professional, or researcher you'll discover inspiring content!You can find Concentrating on Chromatography Podcast in podcast apps:<ul><li><a target="_blank" rel="noopener noreferrer" href="https://open.spotify.com/show/1wZg67rCjoGju4yhqxI3tL">Spotify</a></li><li><a target="_blank" rel="noopener noreferrer" href="https://podcasts.apple.com/tz/podcast/concentrating-on-chromatography/id1788429185">Apple podcast</a></li><li><a target="_blank" rel="noopener noreferrer" href="https://podscan.fm/podcasts/concentrating-on-chromatography">podscan</a></li></ul>and on <a target="_blank" rel="noopener noreferrer" href="https://www.youtube.com/@ChromatographyTalk/videos">YouTube channel</a></blockquote>

N‑Glycans, Whey Protein, and the Gut Microbiome: Mass Spec Insights with Matthew Bolino

<div><div><div>Our immune system is incredibly powerful: among other things, it can recognize and destroy cancer cells. But tumors can be just as effective at avoiding detection – they build “invisibility shields” to protect themselves from attack.</div></div><div>One breakthrough in cancer therapy has been immune checkpoint inhibitors (ICIs), drugs that reactivate immune T cells and help them fight tumors again. However, only about 20–50% of patients respond well to this treatment. Researchers are therefore seeking alternative therapeutic strategies. A small and well-known molecule called adenosine plays a key role in these efforts.When tumor cells are stressed or dying, they release ATP, which is normally a signal that something is wrong in the tissue. But in the tumor microenvironment, ATP gets rapidly broken down by two enzymes, CD39 and CD73. Released adenosine acts like a chemical “calm-down” signal, binds to immune cells (T cells, NK cells, and others), and tells them to slow down, reducing their ability to kill cancer cells. Targeting CD73 therefore represents a promising strategy for developing new cancer treatments.</div><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/UOCHB_Nove_latky_mohou_podporit_imunitni_system_v_boji_s_rakovinou_88c5e82cd5.jpeg" alt="IOCB Prague: Development of potent inhibitors to boost the immune system's ability to fight cancer" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_UOCHB_Nove_latky_mohou_podporit_imunitni_system_v_boji_s_rakovinou_88c5e82cd5.jpeg 245w," sizes="100vw" width="661" height="363">At IOCB Prague, interdisciplinary teams led by Jan Řezáč, Jan Konvalinka, and Michal Hocek used a newly developed high-throughput screening method to test over 5,000 compounds, identifying two ribonucleoside derivatives as promising CD73 inhibitors.The scientists then improved the compounds’ activity by carefully modifying the ribonucleoside scaffold with bulky (het)aryl groups. Further optimization improved the pharmacokinetic properties of these compounds, yielding inhibitors with low clearance, long half-life, high solubility, and strong selectivity over related enzymes such as CD39.In cellular experiments, the compounds reduced adenosine production in cancer cells, restored T cell activation, and showed no toxicity toward human fibroblasts. The most active compound showed a single-digit picomolar inhibition constant (Ki).These findings establish a strong foundation for further in vivo efficacy and combination studies, which will explore the therapeutic potential of this novel inhibitor class.<blockquote><h4>The Original article</h4><h4><a target="_blank" rel="noopener noreferrer" href="https://pubs.acs.org/doi/10.1021/acsptsci.5c00707">Potent Competitive Inhibitors of Ecto-5′-nucleotidase (CD73) based on 6-(Het)aryl-7-deazapurine Ribonucleoside 5′-O-Bisphosphonates</a></h4>Ugnė Šinkevičiu̅tė, Magdalena Šímová, Radek Staník, Lenka Poštová Slavětínská, Kristýna Blažková, Pavel Šácha, Martin Lepšík, Jan Řezáč, Jan Konvalinka, Tereza Ormsby, Michal Tichý, Michal HocekACS Pharmacol. Transl. Sci. 2026, 9, 1, 191–213<a target="_blank" rel="noopener noreferrer" href="https://pubs.acs.org/doi/10.1021/acsptsci.5c00707">https://pubs.acs.org/doi/10.1021/acsptsci.5c00707</a><h6>licensed under <a target="_blank" rel="noopener noreferrer" href="https://creativecommons.org/licenses/by/4.0/">CC-BY 4.0</a></h6></blockquote><h4>Abstract</h4>CD73 generates immunosuppressive adenosine in the tumor microenvironment and is a promising target for cancer immunotherapy. We have designed and systematically studied diverse 2-substituted 7-deazapurine ribonucleoside 5′-O-bisphosphonates bearing a variety of (het)aryl groups at position 6 and discovered their highly potent and selective CD73 inhibition activity. The most active compounds (with single-digit picomolar Ki) contained bicyclic (het)aryl groups at position 6 in combination with chlorine at position 2. Further optimization of pharmacokinetic properties identified inhibitors with low clearance, long half-life, high solubility, and excellent selectivity over CD39 and NTPDase3. They effectively suppressed adenosine formation in MDA-MB-231 cells, rescued CD8+ T cell activation, and were nontoxic to human fibroblasts. Overall, their profile compares favorably with AB680, a CD73 inhibitor currently in phase I/II clinical trials.</div>

Development of potent inhibitors to boost the immune system's ability to fight cancer

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The Institute of Organic Chemistry and Biochemistry of the Czech Academy of Sciences (IOCB Prague) is a leading scientific institution in the Czech Republic, recognized internationally. Its primary mission is basic research in the fields of chemical biology and medicinal chemistry, organic and material oriented chemistry, chemistry of natural compounds, biochemistry and molecular biology, physical chemistry, theoretical chemistry, and analytical chemistry. 

Institute of Organic Chemistry and Biochemistry of the Czech Academy of Sciences

Organomation, founded in 1959, designs and manufactures high-quality nitrogen evaporators and extraction systems. Known for innovation and durability, their lab instruments are used globally for efficient sample preparation with strong customer support.

Organomation

Agilent Technologies

Phenomenex

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Waters Corporation

Accelerating Routine Food &amp; Beverage Testing with Optimized Methods
Explore the latest technology and methodology solutions to help you deliver results more easily and efficiently for routine Food and Beverage QC testing for : Sugars, Sugar Substitutes Additives, Vitamins and Amino Acids.
<h3>EPISODE 3 - Water and Fat Soluble Vitamines Analysis</h3>
<h3>Agenda</h3>
<h4>Develop Water and fat soluble Vitamins Analysis</h4>
<ul>
<li>Presenter: To be announced​</li>
</ul>
Abstract: Available soon
<h5>Live Q&amp;A session</h5>
<ul>
<li>Moderator: Cecile Pinto (Food &amp; Environmental Market Development Manager, South Europe - Waters)</li>
</ul>

Waters Corporation: Analysis of Water and Fat Soluble Vitamins in Food and Beverages​

Analysis of Water and Fat Soluble Vitamins in Food and Beverages​

Topics: Develop Water and fat soluble Vitamins Analysis

Water and Fat Soluble Vitamins in Food and Beverages​

Current paradigms for drug discovery rely heavily on the quick and effective separation of complex biomolecules. Specifically, a post-translational modification known as glycosylation, which involves adding sugar molecules to proteins, has the potential to significantly alter the structure and function of proteins. Historically, it has been difficult to select the appropriate platform for high-resolution analysis of complex N-linked glycans.
We will talk about current strategies for glycan examination of biotherapeutics by HPLC. The introduction of robust method best practices is planned. Method development strategies, including gradient and mobile phase optimization, will be discussed for resolving critical pairs. In addition, we will investigate strategies for increasing glycan resolution and separation.
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Presenter: Mohit Patel (Application Engineer, Agilent Technologies, Inc.)
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Mohit Patel is part of the CSD Online AE Team. His primary focus is to support SMLC, Bio-Columns, and consumables.
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Before joining Agilent, Mohit was an Assistant Professor of Chemistry at Temple University. Mohit developed his passion for science through his experiences as a faculty researcher and a mentor. Mohit actively taught, developed curriculum, and served as senior personnel, with a leadership position, on funded grants, mentored research projects, and co-coordinator of STEAM related outreach services.
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Mohit has earned a Doctorate degree in Chemistry from Temple University in 2016.
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Glycan Analysis Overview, Method Optimization, and Analysis by HPLC

We will talk about current strategies for glycan examination of biotherapeutics by HPLC.

Glycan Analysis Overview, Method Optimization, and HPLC

Critical to the development and commercialization of a biosimilar is understanding the originator and how its CQAs compare against those of the biosimilar.
Learn more about our solutions that provide high quality peptide mapping, glycan and charge variant data.
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Presenter: Andrew Argo (Strategic Marketing Lead, Waters Corporation)
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Andrew has held positions across industry with a focus on analytical method development for LC and LCMS applications for products ranging from peptides to gene therapy for almost 10 years. He is currently the strategic marketing lead for the biopharma and new modality portfolios within the chemistry organization, helping to understand these evolving therapeutic areas and customer needs.
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Waters Corporation: Chromatographic techniques for the characterization of CQAs in biosimilars 

Chromatographic techniques for the characterization of CQAs in biosimilars

Solutions that provide high quality peptide mapping, glycan and charge variant data.

Chromatographic techniques for the characterization of CQAs

Key Learning Points:
<ul>
<li>Acquire knowledge about Instrument and mobile phase bottle cleanliness
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<li>Listen in to a systematic breakdown of isolating the unknown peaks
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<li>Acknowledgment of impurity profile for each brand of Formic Acid
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</ul>
Who Should Attend:
<ul>
<li>Any beginner or intermediate HPLC/UV users, or anyone interested in learning more about HPLC systems that inherent contaminants seen in chromatograms</li>
</ul>
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Presenter: Zeshan Aqeel (Senior Application Scientist, Phenomenex)
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Zeshan Aqeel is part of the Technical Support team at Phenomenex providing problem solving, troubleshooting, and applications support to customers around the world. In this capacity, Zeshan has also assisted in new Phenomenex staff with hands‐on Lab Training and developed and presented many technical seminars as well as demonstrations globally.
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Mr. Zeshan has over 15 years of chromatography experience and is an expert in practical HPLC method development. After graduating from California State University Northridge with a degree in Biochemistry, he worked at Amgen and Baxter Bioscience as an analytical chemist optimizing LC and LC/MS methods.
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Joining Phenomenex in 2010 as part of PhenoLogix HPLC/SPE method development team, Zeshan has had a hand in many of the new marketing LC and SPE methods for flagship products such as Kinetex, Strata, Luna Omega and BioZen SEC. Recently, he has been running SEC-MALS and had data presented at 2023 ASMS in Houston.
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Phenomenex: A Formic Acid Case Study – Troubleshooting Unwanted Peaks in HPLC/UV

A Formic Acid Case Study – Troubleshooting Unwanted Peaks in HPLC/UV

Acquire knowledge about Instrument and mobile phase bottle cleanliness.

Troubleshooting Unwanted Peaks in HPLC/UV

Key Learning Points:
<ul>
<li>Understand how to obtain good resolution by utilizing selectivity
</li>
<li>Learn the basics of different stationary phase particle characteristics 
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<li>Discover how to choose different stationary phases based on compound classes
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<li>Find how mobile phase can be used to change selectivity of columns
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</ul>
Who Should Attend:
<ul>
<li>Any LC users who are looking for are looking for a basic overview of Reverse Phase Method Development by looking at different small molecule compound classes</li>
</ul>
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Presenter: Genevieve Hodson (Senior Application Scientist, Phenomenex)
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Genevieve graduated from the University of Texas with a BS in Chemistry located in her home town of Austin Texas. She spent 2 years of her undergraduate performing research in an Organic Chemistry Lab synthesizing intermediates. Out of school she spent 4 years as an Analytical Chemist in the QC and R&amp;D Department of Cerilliant (a Sigma Millipore Company). There she worked with many analytical instruments but became an expert at LC by performing purity analysis, customer specific methods, validations, qc testing and stability studies on a wide range of small molecules including pharmaceuticals, pesticides, explosives and illegal substances. From there she moved to Los Angeles California where she worked at Johnson and Johnson and then a small dietary supplement company. After Genevieve spent a year living abroad in Israel raising her first son, she moved back to LA where she got hired as a Technical Specialist at Phenomenex. At Phenomenex she enjoys continuing to learn about chromatography every day through helping customer with all their chromatography needs!
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Phenomenex: Understanding Reverse Phase Selectivity for Different Compound Classes

Understanding Reverse Phase Selectivity for Different Compound Classes

Understand how to obtain good resolution by utilizing selectivity.

Understanding Reverse Phase Selectivity

The quality by design (QbD) approach in analytical method development emphasizes risk management and comprehensive scientific approach.
Its application in analytical method lifecycle helps to increase the robustness and ruggedness of methods compared to traditional method development approaches. Furthermore, the United States Pharmacopeia (USP) Chapter &lt;1220&gt; provides the framework for the implementation of QbD principles around analytical method. USP &lt;1220&gt; emphasizes the importance of sound scientific approaches and quality risk management for the development, control, establishment and use of analytical procedures.
In this webinar, Dr. Rajagopal Karuppuswamy, Vice President of analytical servies at Nephron Pharmaceuticals Corporation and Manu Grover, business development manager of pharma markets at Agilent Technologies will discuss the concept of QbD in reference to analytical development, the steps required to implement QbD, recent regulations in this area, and the benefits of adopting QbD.
Key learning objectives
<ul>
<li>Learn about the implementation of QbD in research labs and the analytical development process
</li>
<li>Gain insight into improving method robustness and implementing risk assessment approaches during analytical development
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<li>Explore the steps required to comply with regulatory guidelines and the lifecycle management approach
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</ul>
Who should attend?
<ul>
<li>Analytical scientists
</li>
<li>Lab managers
</li>
<li>Regulatory experts
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<li>R&amp;D heads
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<li>Quality control scientists/managers
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<li>Quality assurance experts
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</ul>
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Presenter: Dr. Rajagopal Karuppuswamy (Vice President - Analytical Services, Nephron Pharmaceuticals Corporation, West Columbia, South Carolina)
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Dr. Raja Karuppusamy gained his MS in pharmaceutics and PhD in analytical chemistry from the University of Delhi, India. He has over 20 years’ experience in the pharmaceutical industry. He is currently working as the Vice President of Analytical Services with the Nephron Pharmaceuticals Corporation in South Carolina. He has extensive experience with various regulatory agencies such as the Food and Drug Administration, Medicines and Healthcare products Regulatory Agency, and the Brazilian Health Regulatory Agency (Anvisa). He has published over 20 research papers and has contributed toward multiple USP monographs.
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Presenter: Manu Grover (Business development manager – Pharma markets, Agilent Technologies)
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Manu Grover is a postgraduate in pharmacy from the Manipal Academy, India. He has 21 years of chromatography experience and has worked in pharmaceuticals to support investigational new drug applications, new drug applications and abbreviated new drug application filings. During his career, he has developed and validated analytical methods on HPLC, GC, particle sizing, DSC, pXRD and LC/MS. In his current role he works with the Agilent sales team, regulators and pharmaceutical scientists. He shares market updates, trends and expertise related to Agilent’s portfolio of columns supplies, and helps to ensure scientists select the right solutions for their lab.
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Presenter: Blake Forman (Scientific Content Creator, SelectScience)
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Blake studied chemistry at the University of Surrey, UK, and further went on to study a master’s in chemistry at the University of Southampton, UK. Blake has previously worked in the Medicines and Healthcare Products Regulatory Agency (MHRA) lab at LGC and as a technical sales specialist for Waters. As a member of the Editorial team, he now plays an integral role in shaping the content on SelectScience.
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SelectScience: Analytical method development: A quality by design (QbD) approach

Analytical method development: A quality by design (QbD) approach

Concept of QbD in reference to analytical development, the steps required to implement QbD, recent regulations in this area, and the benefits of adopting QbD.

Analytical method development: A quality by design approach

How can you maximize the productivity of your trusted Waters liquid chromatography (LC) systems?
With the easy-to-use Waters Automation Portal, you will be able to automate your ACQUITY and Arc UPLC, UHPLC, and HPLC Systems to reduce time spent on tedious manual procedures, minimize errors, and making your LC and LC-MS workflows more efficient.
In this webinar, hear from Waters partners who will explain how installing the Automation Portal onto their ACQUITY and Arc Sample Managers simplified sample transfer, allowing their scientists to focus on the science instead of tasks.
The Automation Portal is configurable with a wide range of Waters LC and LC-MS systems, reduces space needed for plate loading and transfer, enables automation scheduling with specially designed software, and conveniently works with your preferred integrator to fit into your established workflows.
Join our webinar to see how to streamline your workflows and achieve the quality and consistency your lab demands from its automation partner.
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Presenter: Darcy Shave (Principal Product Manager, Waters Corporation)
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Darcy Shave is currently a principal product manager in the Small Molecules group at Waters Corporation (Milford, MA, USA), responsible for the purification and hardware automation products. Darcy has been with Waters for over 20 years, starting as an applications chemist in the purification group. From there, he moved into a variety of business development roles before becoming a product manager for purification software about 10 years ago and more recently for the entire purification portfolio. He has a degree in Agricultural Chemistry from the University of Saskatchewan and worked in value added processing of natural products before joining Waters.
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Presenter: John Canty (Technical Trainer, Biosero)
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John Canty is the Technical Trainer at Biosero where he educates scientists, automation engineers, and developers in automation solutions. With 19 years of experience in automation, John has held engineering roles at many other companies within the industry, including HighRes Biosolutions, Labcyte, Beckman Coulter, Zymergen, and Illumina. Throughout his career, he has been responsible for the concept, design, programming, and testing of automated equipment and processes. He now uses his expertise to teach others how to master automation software and technology for their own work.
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Waters Corporation: Automate and Streamline Your Lab’s LC and LC-MS Workflows

Automate and Streamline Your Lab’s LC and LC-MS Workflows

We will explain how installing the Automation Portal onto ACQUITY and Arc Sample Managers simplified sample transfer.

The webinar is for novice and experienced LC users, who want to refresh their knowledge on the best practices of working with LC instrumentation.
Join Thermo Fisher Scientific experts on the flow path journey from sample injection to detection to learn useful tips on:
<ul>
<li>Learn what might impact your chromatographic separation and detection, before you start your work.
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<li>How to choose the right configuration and settings to achieve the best results.
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<li>How to keep your system healthy and running.
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</ul>
By attending this webinar, you will learn to optimize LC operations to achieve the best results for your LC separation.
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Presenter: Dr. Susanne Fabel (HPLC Product Marketing Manager, Thermo Fisher Scientific)
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Susanne Fabel is a Product Marketing Manager in the Chromatography and Mass Spectrometry group at Thermo Fisher Scientific. Her passion is to help develop world-class HPLC instruments to take science one step further. She has more than 15 years of experience in chromatography and holds a PhD in analytical chemistry from the Technical University of Munich.
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Presenter: Mr. Sumit Verma (Regional Program Manager, Thermo Fisher Scientific)
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Sumit Verma, is a distinguished Product Market Manager in the Asia-Pacific and Japan region. He is deeply associated with Chromatography and Mass spectrometry at Thermo Fisher Scientific, where he has amassed 18 years of rich experience in the Analytical Industry. Armed with a Post Graduation in Electronics and an Management studies from IIMA India, Sumit embarked on his professional journey as a service engineer before venturing into Sales. Now, as a Product Marketing expert, he thrives on collaborating closely with customers to drive product improvement and innovation, aiming to simplify and enrich their lives.
</blockquote>

Thermo Scientific: Back to Basics – Tips and tricks of HPLC

Back to Basics – Tips and tricks of HPLC

Learn to optimize LC operations to achieve the best results for your LC separation.

Amino Acid Analysis is required in many applications related to foods, dietary supplements and animal feeds and is a fundamental tool for monitoring product QC and compliance with nutritional labeling.
AOAC International has recently published a standardized method (AOAC Official Method 2018.06) for the analysis of total amino acids in infant formulas and adult nutritionals by UHPLC-UV. For some samples, to obtain a full amino acid profile, the samples must first be hydrolyzed. Due to the varied properties of amino acids and diversity of sample matrices, hydrolysis, separation and detection represents an analytical challenge. Learn more about effective hydrolysis, separation and detection of amino acids in dietary supplements, plant-based meats, and pet food during a 3-part webinar series with CEM and Waters.
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Presenter: Alicia Douglas Stell, Ph.D. (Market Development Manager, Applied Markets, CEM Corporation)
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Dr. Alicia Douglas Stell has worked with CEM for the past 15 years, joining after completing her PhD in Analytical Chemistry. Her extensive background in chromatographic analysis and her mechanical expertise have been integral in the development of both instrumentation and novel applications. In her role as Market Development Manager, she is continuously exploring new applications and markets for innovative technology, focused around sample preparation.
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<blockquote>
Presenter: Benedict Liu (Product Specialist, Organic Solutions, CEM Corporation)
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<blockquote>
Benedict Liu graduated from UNC Chapel Hill in 2018 with a bachelor&#39;s degree in chemistry and mathematics. He joined CEM soon after and has been with the company for 5 years serving as an applications chemist and product specialist for the Molecular Sample Preparation division. In his time at CEM, Ben has worked to develop solutions for the extraction of organic pollutants in soils and product, microwave amino acid hydrolysis protocols, and automated extraction methods for PFAS in solid and semi-solid matrices.
</blockquote>
<blockquote>
Presenter: Paula Phenix (Principal Product Marketing Manager, Waters Corporation)
</blockquote>
<blockquote>
Paula is a Principal Product Marketing Manager at Waters with a focus on products for amino acid and glycan analysis. Prior to her current role in the Chemistry and Lab Automation Group, she was the Product Marketing Manager for Specialty LC and SFC Systems in the Instruments group. Before joining Waters, she held product management, marketing and sales roles for several companies (Life Technologies, Promega, MilliporeSigma) in the molecular biology, immunology, cell culture and single-use bioprocessing areas. Paula graduated from the University of Delaware with a Bachelor’s degree in Biology and from Johns Hopkins University with a Master’s degree in Business.
</blockquote>
<blockquote>
Presenter: Ravali Alagandula, Ph.D. (Chemistry Market Development Manager, Waters Corporation)
</blockquote>
<blockquote>
Ravali is a Chemistry Market Development Manager at Waters with focus on all chemistry consumables and lab automation products. She holds a Ph. D. in Analytical Chemistry from Cleveland State University. She started her career as an LC applications scientist at Restek, developing LC-MS/MS and sample preparation methods in clinical and life science markets. Most recently, Ravali was a Chemistry Account Manager at Waters supporting key accounts and customers to promote chemistry consumable business in all market areas.
</blockquote>

Waters Corporation: Easy & Efficient Amino Acid Sample Prep followed by Accurate Detection - a look at Wet and Dry Pet Food

Easy & Efficient Amino Acid Sample Prep followed by Accurate Detection - a look at Wet and Dry Pet Food

Learn more about effective hydrolysis, separation and detection of amino acids in dietary supplements, plant-based meats, and pet food.

A look at Wet and Dry Pet Food

Waters Corporation: Easy & Efficient Amino Acid Sample Prep followed by Accurate Detection - a look at Plant-based Meats and Proteins

LCMS webinars focusing on HPLC - page 32

Back to Basics – Tips and tricks of HPLC

A Formic Acid Case Study – Troubleshooting Unwanted Peaks in HPLC/UV

Easy & Efficient Amino Acid Sample Prep followed by Accurate Detection - a look at Wet and Dry Pet Food

Analysis of Water and Fat Soluble Vitamins in Food and Beverages

Chromatographic techniques for the characterization of CQAs in biosimilars

Understanding Reverse Phase Selectivity for Different Compound Classes

Automate and Streamline Your Lab’s LC and LC-MS Workflows

Easy & Efficient Amino Acid Sample Prep followed by Accurate Detection - a look at Plant-based Meats and Proteins

Analytical method development: A quality by design (QbD) approach

Glycan Analysis Overview, Method Optimization, and Analysis by HPLC

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Easy & Efficient Amino Acid Sample Prep followed by Accurate Detection - a look at Wet and Dry Pet Food

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Analytical method development: A quality by design (QbD) approach

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