Join us for a fascinating conversation with Dr. Subhoja Chakraborty from the University of Central Florida, whose cutting-edge research targets the global challenge of malaria drug resistance. Dr. Chakraborty shares her journey from structure-based drug design during her PhD to her recent collaborative work repurposing human kinase inhibitors as rapid, selective antimalarials against drug-resistant Plasmodium falciparum strains.<h4>In this episode, you'll learn:</h4><ul><li>How structure-guided approaches reveal potential drug targets, including cysteine proteases like falcipain-2, and kinases implicated in malaria's complex life cycle.</li><li>The power of repurposing kinase inhibitors originally developed for cancer and other diseases, focusing on compound 12, which blocks hemozoin formation and protein kinase 6—key steps in parasite survival.</li><li>Lab techniques such as affinity, gel filtration, and size exclusion chromatography for recombinant protein purification—and how troubleshooting is crucial for high-yield, stable complexes.</li><li>Advances in mass spectrometry for characterizing protein interactions, validating drug targets, and mapping molecular pathways pivotal to malaria therapeutics.</li><li>Tips and lessons for new researchers facing the challenges of sample preparation, complex protocols, and working as part of a collaborative team.</li></ul>Dr. Chakraborty’s insights bridge the gap between basic science and translational drug discovery, offering hope for more effective, targeted malaria treatments in a world where resistance to frontline therapies threatens millions.<h4>Referenced Papers:</h4><ul><li>"Plasmodium falciparum protein kinase 6 and hemozoin formation are inhibited by a type II human kinase inhibitor exhibiting antimalarial activity" (Cell Chemical Biology, 2025)</li><li>"New insights of falcipain 2 structure from Plasmodium falciparum 3D7 strain" (BBRC, 2022)</li><li>"Structure-Based Optimization of Protease−Inhibitor Interactions to Enhance Specificity of Human Stefin‑A against Falcipain‑2 from the Plasmodium falciparum 3D7 Strain" (Biochemistry, 2023)</li></ul>If you’re a student, scientist, or simply passionate about advances in infectious disease research, this episode offers actionable insights, inspiring advice, and a front-row seat to innovation in combating malaria.<h3>Video Transcription</h3><h4>From Structural Biology to Drug Discovery: Chromatography and Mass Spectrometry in Malaria Research</h4><h5>Introduction</h5>In this discussion, Subhoja shares insights into her research journey, focusing on malaria drug discovery and the role of modern analytical techniques in biological and biomedical research. The conversation is aimed primarily at undergraduate students and early-career researchers interested in chemistry, biotechnology, and life sciences.<h5>Research Focus: Malaria Drug Discovery and Kinase Targets</h5>Subhoja is currently involved in malaria drug discovery research at the University of Central Florida, where she has been working since beginning her postdoctoral position two years ago. Her earlier PhD research focused on structure-based drug design in malaria, using a combination of in silico approaches, recombinant protein expression, and X-ray crystallography to determine precise three-dimensional structures of target proteins and their inhibitors.This structural perspective provided a foundation for her current work, which centers on drug discovery through compound screening and repurposing. A recent publication from her laboratory explores the use of repurposed mammalian CDK-like kinase inhibitors as potential antimalarial agents. While such kinase inhibitors are well known in cancer and cardiovascular research, their application to malaria targets represents a novel and promising approach.Kinases are attractive drug targets because of their central role in cell signaling and growth pathways. By targeting specific kinases involved in different stages of the malaria parasite life cycle—whether asexual growth, gametocytogenesis, or differentiation—it may be possible to significantly inhibit parasite growth and progression.<h5>Chromatography in Protein Expression and Purification Workflows</h5>Chromatography plays a critical role throughout Subhoja’s research. Her experience spans from basic chromatography techniques during her early training to advanced protein purification workflows using affinity chromatography, gel filtration (size exclusion chromatography), and ion exchange chromatography.Recombinant proteins are routinely purified using affinity resins such as nickel or cobalt, followed by further purification to remove contaminants. Fast Protein Liquid Chromatography (FPLC) enables compact, stepwise purification workflows suitable for producing proteins for in vitro assays.A recurring challenge in this process is the expression of high-molecular-weight proteins, which often accumulate in insoluble fractions or inclusion bodies. Careful selection of expression vectors, host systems, and induction conditions is therefore essential to maximize yield and solubility.<h5>Integrating Size Exclusion Chromatography into the Workflow</h5>Size exclusion chromatography (SEC) is typically used after affinity purification to further improve protein purity. Following elution with imidazole or glutathione, desalting is required to prevent buffer components from interfering with downstream assays. This is often achieved using desalting columns such as G-25 or PD-10.To remove lower-molecular-weight contaminants, finer SEC media—such as Superdex or Sephacryl columns—are employed. Concentration steps using molecular-weight cut-off filters are then applied to obtain the desired protein concentration for functional studies.These iterative purification and troubleshooting steps are a routine part of protein biochemistry and reflect the importance of methodical optimization and careful validation.<h5>The Role of Mass Spectrometry in Biomolecular Characterization</h5>Mass spectrometry is a key analytical tool in Subhoja’s research pipeline, particularly for target identification and validation. In both malaria and cancer research, MS is used alongside chromatography, immunoprecipitation, and pull-down assays to study protein interactions and molecular recognition events.Affinity-based mass spectrometry enables the identification of binding partners and interactors, whether studying proteins, long non-coding RNAs, or protein complexes. Techniques such as native MS, tandem affinity purification, and proximity labeling (e.g., TurboID-based workflows) provide deeper insights into molecular networks and signaling pathways.These approaches are essential for understanding how candidate drug targets function within complex biological systems.<h5>Sample Preparation Challenges in Biological Research</h5>Sample preparation remains one of the most challenging aspects of biological experimentation. Whole-cell lysates contain proteases, nucleases, and other components that can rapidly degrade target molecules. Preventing degradation requires minimizing preparation time, maintaining low temperatures, and using appropriate inhibitor cocktails.Reproducibility is also critical: biological experiments require multiple replicates, and variability is often unavoidable. Careful handling, protocol optimization, and continuous troubleshooting are essential to ensure reliable results, whether preparing samples for chromatography, mass spectrometry, sequencing, or immunoprecipitation.<h5>Advances in Chromatography and MS for Malaria Research</h5>Modern advances in chromatography and mass spectrometry significantly enhance malaria research by enabling precise target identification, quantification, and functional characterization. Drug discovery workflows begin with construct design and recombinant expression, followed by purification and interaction studies.In malaria research, stage-specific parasite biology requires careful extraction and analysis of proteins from different life cycle stages. Tandem affinity MS, immunoprecipitation, and in vivo validation studies help confirm target relevance and therapeutic potential.Recent work on repurposed kinase inhibitors has shown promising results, including inhibition of hemozoin formation—a key detoxification pathway for the malaria parasite—highlighting the potential of such compounds for further development.<h5>Malaria, Drug Resistance, and Global Impact</h5>Malaria remains a major global health burden, with over 600,000 deaths annually and widespread drug resistance complicating treatment. Artemisinin resistance, particularly associated with mutations in the K13 propeller domain, has driven the search for new targets and therapies.During her PhD, Subhoja focused on falcipain-2, a cysteine protease involved in hemoglobin degradation within the parasite. This pathway is critical for parasite survival and has emerged as a promising drug target. Her work revealed that falcipain-2 operates within a larger protein complex responsible for hemoglobin processing and amino acid release.Understanding these molecular mechanisms is essential for developing robust, stage-specific antimalarial strategies.<h5>Advice for Early-Career Researchers</h5>For researchers just starting out, Subhoja emphasizes the importance of hands-on experience. Working directly with cells, lysates, and analytical tools is essential for truly understanding experimental systems. Failure is an inherent part of research, and perseverance is key.Motivation, teamwork, careful sample handling, and thorough preparation all contribute to long-term success. Reading extensively, critically evaluating one’s own work, and appreciating incremental progress are vital aspects of a sustainable research career.Every successful experiment—no matter how small—represents meaningful progress and contributes to the broader goals of science and medicine.<h5>Closing Remarks</h5>The discussion highlights how interdisciplinary approaches combining chromatography, mass spectrometry, structural biology, and biochemistry are driving advances in malaria drug discovery. Through careful experimental design, analytical rigor, and collaborative teamwork, researchers can address complex biological challenges and contribute to global health solutions.<blockquote>This text has been automatically transcribed from a video presentation using AI technology. It may contain inaccuracies and is not guaranteed to be 100% correct.</blockquote><blockquote><h4>Concentrating on Chromatography Podcast</h4>Dive into the frontiers of chromatography, mass spectrometry, and sample preparation with host David Oliva. Each episode features candid conversations with leading researchers, industry innovators, and passionate scientists who are shaping the future of analytical chemistry. From decoding PFAS detection challenges to exploring the latest in AI-assisted liquid chromatography, this show uncovers practical workflows, sustainability breakthroughs, and the real-world impact of separation science. Whether you’re a chromatographer, lab professional, or researcher you'll discover inspiring content!You can find Concentrating on Chromatography Podcast in podcast apps:<ul><li><a target="_blank" rel="noopener noreferrer" href="https://open.spotify.com/show/1wZg67rCjoGju4yhqxI3tL">Spotify</a></li><li><a target="_blank" rel="noopener noreferrer" href="https://podcasts.apple.com/tz/podcast/concentrating-on-chromatography/id1788429185">Apple podcast</a></li><li><a target="_blank" rel="noopener noreferrer" href="https://podscan.fm/podcasts/concentrating-on-chromatography">podscan</a></li></ul>and on <a target="_blank" rel="noopener noreferrer" href="https://www.youtube.com/@ChromatographyTalk/videos">YouTube channel</a></blockquote>

Affinity, Gel Filtration, Size Exclusion Chromatography: Repurposing Kinase Inhibitors for Malaria

In the week of February 9, the following webinars await you in the field of liquid phase and especially HPLC and LC/MS.<blockquote><h4><a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/webinars">👉 DETAILS AND REGISTRATION IN THE WEBINARS SECTION</a></h4>👉 Search and filter in the largest global database with over 5 000 GC, LC, MS and spectroscopy webinars.</blockquote><h5>1. SelectScience: High-Throughput Stability Screening Strategies for Antibody Formulation</h5><ul><li>Tu, 10.2.2026 17:00 CET</li><li><a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/webinar/5807">Registration</a></li></ul>Learn how high-throughput DLS/SLS and rapid-screening DSC accelerate stability assessment of high-concentration antibody formulations, enabling faster, data-driven biologics development.<h5>2. Phenomenex: Big Scale, Big Wins – Prep Chromatography Explained</h5><ul><li>We, 11.2.2026 10:00 CET</li><li><a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/webinar/5793">Registration</a></li></ul>Learn how to scale analytical methods to preparative HPLC, optimize loading capacity, and understand the limits of preparative columns for pharmaceutical applications.<h5>3. ALS Czech Republic: PFAS in Food &amp; Food Contact Material</h5><ul><li>We, 11.2.2026 15:00 CET</li><li><a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/webinar/5816">Registration</a></li></ul>Get a practical overview of PFAS in food and food contact materials, including EU and US regulations, key analytical challenges, and reliable PFAS testing strategies.<h5>4. New Food Magazine: The Science of Next-Gen Ingredients – Fermentation, Formulation and Function</h5><ul><li>We, 11.2.2026 16:00 CET</li><li><a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/webinar/5720">Registration</a></li></ul>Explore how fermentation-driven innovation is transforming functional food ingredients and how advanced analytics optimize flavour, functionality, and stability.<h5>5. SCIEX: Accurate Mass Fundamentals – Interpreting and Applying Your Data</h5><ul><li>We, 11.2.2026 19:00 CET</li><li><a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/webinar/5772">Registration</a></li></ul>Session 3 focuses on interpreting and applying accurate mass data to confirm compound identity, move beyond basic quantitation, and troubleshoot results for confident reporting.<h5>6. Agilent Technologies: Process Analytical Techniques – Online HPLC for Real-Time Monitoring and Control of Bioprocesses</h5><ul><li>Th, 12.2.2026 06:30 CET</li><li><a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/webinar/5834">Registration</a></li></ul>Discover how PAT HPLC enables near-real-time monitoring of critical quality attributes in bioprocessing, with a case study on charge-variant control by cation-exchange chromatography.<h5>7. Thermo Fisher Scientific: Introducing the Orbitrap Excedion Pro Hybrid MS</h5><ul><li>Th, 12.2.2026 11:00 CET</li><li><a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/webinar/5889">Registration</a></li></ul>Discover the new Orbitrap Exploris Pro with expanded ETD/EThcD capabilities for confident biopharmaceutical characterization, including intact proteins, PTMs, ADCs, and large biomolecules.<h5>8. Waters Corporation: Planning Your NuGenesis Upgrade – Insights and Best Practices for a Smooth Transition</h5><ul><li>Th, 12.2.2026 19:00 CET</li><li><a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/webinar/5833">Registration</a></li></ul>Learn how to plan a successful NuGenesis upgrade, transition to Windows 11, and unlock new capabilities such as dashboards for real-time operational insights.<h5>9. Thermo Fisher Scientific: Comprehensive Analytical Strategies for Peptide Therapeutics and GLP-1 Agonists with Agilent</h5><ul><li>Th, 12.2.2026 19:00 CET</li><li><a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/webinar/5814">Registration</a></li></ul>Explore analytical workflows for peptide therapeutics, including method development, peptide mapping, GLP-1 QC, and LC-MS strategies across Agilent platforms.<h5>10. Agilent Technologies: Moving from R&amp;D and Limited QC on a UV-Vis-NIR Instrument to Large-Scale QC Using an Autosampler</h5><ul><li>Th, 12.2.2026 20:00 CET</li><li><a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/webinar/2104">Registration</a></li></ul>This webinar covers practical aspects of adding and using an autosampler to maximize UV-Vis-NIR QC workflows at scale.<h5>11. Agilent Technologies: LC Method Transfer – Hidden Challenges &amp; Absolute Solution</h5><ul><li>Fr, 13.2.2026 06:30 CET</li><li><a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/webinar/5787">Registration</a></li></ul>Discover why LC method transfer between legacy and modern systems is challenging, which hidden parameters affect retention and peak shape, and how to achieve robust, reliable results.

Webinars LabRulezLCMS Week 07/2026

Charge detection mass spectrometry (CDMS) allows direct mass determination of individual ions and is increasingly used to study heterogeneous biological samples. This work demonstrates the use of Orbitrap-based CDMS/Direct Mass Technology (DMT) for quantitative analysis of transthyretin (TTR) tetramer samples below 100 kDa, extending CDMS beyond its traditional qualitative role.DMT resolved wild-type and C-terminally modified TTR homotetramers, as well as hybrid tetramers, enabling kinetic analysis of subunit exchange and stability. The approach also quantified thyroxine binding to TTR variants, revealing differences in binding affinity linked to C-terminal modification. By separating overlapping m/z signals through charge measurement, CDMS enables accurate mass and abundance determination, highlighting its potential for quantitative analysis of complex protein systems.<blockquote><h4>The original article</h4><h4><a target="_blank" rel="noopener noreferrer" href="https://pubs.acs.org/doi/10.1021/acs.analchem.5c05771">Dissecting Heterogeneous Populations of Protein-Complex Samples Using Direct Mass Technology</a></h4>Robert L. Rider, Jared Hampton, Zhenyu Xi, Carter Lantz, Sangho D. Yun, Weijing Liu, Rosa Viner, Arthur Laganowsky, and David H. Russell*Anal. Chem. 2025, 97, 49, 27057–27063<a target="_blank" rel="noopener noreferrer" href="https://doi.org/10.1021/acs.analchem.5c05771">https://doi.org/10.1021/acs.analchem.5c05771</a><h6>licensed under <a target="_blank" rel="noopener noreferrer" href="https://creativecommons.org/licenses/by/4.0/">CC-BY 4.0</a></h6></blockquote>Selected sections from the article follow. Formats and hyperlinks were adapted from the original.Transthyretin (TTR) is a homotetrameric transport protein that circulates in plasma and cerebrospinal fluid, delivering thyroxine (T4) and retinol-binding protein–vitamin A complexes. <a href="javascript:void(0);">(1)</a> The stability of the TTR tetramer is central to its physiological role because dissociation into monomers can initiate misfolding and amyloid formation, leading to systemic or hereditary transthyretin amyloidosis. <a href="javascript:void(0);">(2)</a> Subunit exchange between wild-type (WT) and variant TTR proteoforms, including disease-associated mutations such as V30M and V122I, have been implicated in amyloid progression by generating hybrid tetramers (tetramers containing WT and mutant subunits) with altered stabilities. <a href="javascript:void(0);">(3,4)</a> Ligand binding, particularly at the two thyroxine-binding sites located at the dimer–dimer interface, can kinetically stabilize the tetramer and modulate amyloidogenicity. <a href="javascript:void(0);">(5,6)</a> Accurate measurement of both subunit exchange kinetics and ligand binding stoichiometry is therefore critical for elucidating the TTR dynamics and stability.Affinity or sequence tags are widely employed in protein expression, purification, or detection. <a href="javascript:void(0);">(7,8)</a> While these tags are often assumed to be inert, their influence on protein structure and dynamics are rarely examined in detail. <a href="javascript:void(0);">(9,10)</a> Protein tags at the N- and C-terminus of TTR have been shown to have differing gas phase stabilities from those of WT-TTR. <a href="javascript:void(0);">(11)</a> The C-terminus is of particular interest because this region participates in the dimer–dimer interface that governs tetramer stability. <a href="javascript:void(0);">(11,12)</a> Characterizing how the C-terminal tag (CT) (-ASGENLFYQ) affects TTR’s solution stability and ligand binding is not only relevant for understanding TTR dynamics but also provides an example of how expression tags can alter protein dynamics. <a href="javascript:void(0);">(13)</a>Native mass spectrometry (nMS) has been invaluable for characterizing protein complexes, <a href="javascript:void(0);">(14)</a> yet conventional nMS often struggles with heterogeneous samples where overlapping charge state distributions and adduct heterogeneity limit resolution and quantitation. <a href="javascript:void(0);">(15)</a> Charge detection mass spectrometry (CDMS) addresses these limitations by independently measuring the charge and m/z of individual ions, enabling the direct determination of molecular mass and abundance. Development of CDMS has largely focused on achieving higher mass resolution <a href="javascript:void(0);">(16)</a> and extending measurements toward assemblies into the GDa range. <a href="javascript:void(0);">(17,18)</a> However, there is untapped utility for CDMS experiments other than mass and proteoform determination. Jordan et al. showed the capability to assess conformational heterogeneity of mAb aggregates using the added charge dimension that CDMS provides. <a href="javascript:void(0);">(19)</a> The current work demonstrates that CDMS can separate proteoforms with overlapping m/z values based on charge and quantify their relative abundances in complex mixtures. While nMS is already a highly quantitative technique, our results establish CDMS as an equally powerful tool for quantitative analysis in heterogeneous systems. <a href="javascript:void(0);">(20,21)</a>Here, we apply Orbitrap-based CDMS/Direct Mass Technology (DMT) using the selective temporal overview of resonant ions (STORI) approach <a href="javascript:void(0);">(22,23)</a> to quantitatively compare WT-TTR and CT-TTR, focusing on two biologically relevant properties: tetramer stability assessed via subunit exchange kinetics and ligand-binding affinity. Conventional nMS cannot fully resolve the overlapping charge states of WT- and CT-TTR or their ligand-bound states. However, DMT can resolve these species by directly measuring the mass (m/z × charge), enabling quantitation of all proteoforms. The data reveal that the CT tag alters tetramer stability and thyroxine-binding affinity, demonstrating the structural sensitivity of TTR to modifications at its C-terminus. More broadly, this work demonstrates the impact that protein tags can have on native protein interactions and highlights the broader utility of quantitative CDMS for probing heterogeneous biological samples.<h4>Experimental Section</h4><h5>Data Collection</h5>All experiments were conducted on a <a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/products/560">Thermo Scientific Q-Exactive UHMR</a> with added DMT capabilities (Bremen, Germany). Before DMT, mass spectra were collected at various resolving powers (6–200k) for each sample, with the instrument parameters reported in the Supporting Information (<a target="_blank" rel="noopener noreferrer" href="https://pubs.acs.org/doi/suppl/10.1021/acs.analchem.5c05771/suppl_file/ac5c05771_si_001.pdf">Table S1</a>). For DMT experiments, the trapping gas pressure in the HCD cell was reduced to 0.3–0.8 to attenuate the signal intensity to a single ion level, as well as decreasing the UHV pressure to assist ion retention through the entire transient of Orbitrap analysis. We observed no significant difference between mass spectra collected at various HCD cell pressures (Supporting Information, <a target="_blank" rel="noopener noreferrer" href="https://pubs.acs.org/doi/suppl/10.1021/acs.analchem.5c05771/suppl_file/ac5c05771_si_001.pdf">Figure S1</a>). For DMT collection, automated ion control (AIC) and 100% target density were used with 200k resolving power.<h4>Results and Discussion</h4>CDMS has emerged as a powerful tool for analyzing heterogeneous biomolecular samples, enabling accurate, direct mass determination by simultaneously measuring the charge and m/z for individual ions. While CDMS has been widely applied for qualitative analyses, its potential for quantitative and less than MDa applications remains underexplored. <a target="_blank" rel="noopener noreferrer" href="https://pubs.acs.org/doi/10.1021/acs.analchem.5c05771#fig1">Figure 1</a>A shows the theoretical mass spectrum of WT-TTR (14+ to 16+) and CT-TTR (15+ to 17+) homotetramers, where the two proteoforms become increasingly resolved as the charge states increase (&lt;3900 m/z). <a target="_blank" rel="noopener noreferrer" href="https://pubs.acs.org/doi/10.1021/acs.analchem.5c05771#fig1">Figure 1</a>B zooms in on the 14+ (WT) and 15+ (CT) charge states, highlighting their strong overlap, which prevents accurate deconvolution of the proteoforms by conventional methods. <a target="_blank" rel="noopener noreferrer" href="https://pubs.acs.org/doi/10.1021/acs.analchem.5c05771#fig1">Figure 1</a>C displays theoretical distributions for 0, 1, and 2 T4 bound states of both WT- and CT-TTR tetramers, where &lt;3900 m/z has observable separation between all proteoforms. However, for the 14+ and 15+ charge states of WT- and CT-TTR, respectively, the 0 T4- and 1 T4-bound populations overlap (<a target="_blank" rel="noopener noreferrer" href="https://pubs.acs.org/doi/10.1021/acs.analchem.5c05771#fig1">Figure 1</a>D). Furthermore, commonly adducted species (Na+, K+, and Zn2+) observed in experimental spectra broaden these distributions, making it increasingly difficult to distinguish and deconvolute the proteoforms (see <a target="_blank" rel="noopener noreferrer" href="https://pubs.acs.org/doi/suppl/10.1021/acs.analchem.5c05771/suppl_file/ac5c05771_si_001.pdf">Figure S2</a>). Here, we employ Orbitrap-based CDMS/DMT to overcome this m/z overlap to resolve and quantify hybrid tetramer formation and T4 binding.<img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Anal_Chem_2025_97_49_27057_27063_Figure_1_A_Theoretical_mass_spectra_of_WT_TTR_and_CT_TTR_homotetramers_47232849d9.jpg" alt="Anal. Chem. 2025, 97, 49, 27057–27063: Figure 1. (A) Theoretical mass spectra of WT-TTR and CT-TTR homotetramers. (B) Zoomed-in view of the 14+ WT-TTR and 15+ CT-TTR distributions to show the large overlap of the proteoforms. (C) Theoretical mass spectra of WT-TTR and CT-TTR homotetramers with thyroxine (T4) binding. (D) Zoomed-in view of the 14+ WT-TTR and 15+ CT-TTR distributions where the 0 and 1 T4 have overlapping distributions. These theoretical distributions do not contain any commonly adducted species (Na+, K+, Zn2+), which broaden the distributions and create more overlap even for the proteoforms in the range 3300–3900 m/z (see Figure S2)." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Anal_Chem_2025_97_49_27057_27063_Figure_1_A_Theoretical_mass_spectra_of_WT_TTR_and_CT_TTR_homotetramers_47232849d9.jpg 187w," sizes="100vw" width="1417" height="1182"><h5>Thyroxine Binding to TTR Homotetramers</h5>Thyroxine is the less active form of a thyroid hormone important for metabolism that binds to TTR for transport throughout plasma and cerebral spinal fluid. <a href="javascript:void(0);">(31)</a> Turnover of T4 into T3 (active) occurs after dissociation, where it can then modulate gene expression via binding to nuclear receptors. <a href="javascript:void(0);">(32)</a> The TTR tetramer gains substantial stability as it binds up to 2 T4 at the tetramer interface, decreasing tetramer dissociation and SUE. <a href="javascript:void(0);">(33)</a> This has been shown previously to decrease the amyloidogenic rate of TTR, which led to tafamidis (similar in structure to T4) to be designed and is currently the only drug that is approved for clinical treatment of TTR amyloidosis. <a href="javascript:void(0);">(34)</a> Measuring the difference in binding affinity between TTR proteoforms may provide insight into regions of TTR or TTR intermolecular/intramolecular interactions that are important for binding of small molecules such as tafamidis and T4.DMT was further applied to quantify T4 binding to WT- and CT-TTR homotetramers, highlighting the technique’s utility in probing protein–ligand interactions. <a target="_blank" rel="noopener noreferrer" href="https://pubs.acs.org/doi/10.1021/acs.analchem.5c05771#fig3">Figure 3</a>A shows a representative mass spectrum of TTR homotetramers in the presence of T4. While ligand-bound species can be observed, homotetramers cannot be confidently resolved across the entire 3000–5000 m/z range. By leveraging the charge-resolving capabilities of DMT, we achieve separation of the homotetramers (<a target="_blank" rel="noopener noreferrer" href="https://pubs.acs.org/doi/10.1021/acs.analchem.5c05771#fig3">Figure 3</a>B,C) and the 0, 1, and 2 ligand-bound states. The 2D plot clearly shows the gain in resolution achieved when charge is simultaneously measured with m/z by separating the 14+ and 15+ charge states for WT and CT, respectively, in the range 3900–4200 m/z. Additionally, using ion mobility does not provide the same kind of 2D resolution due to the similarity in arrival times for the homotetramer proteoforms, as evidenced by <a target="_blank" rel="noopener noreferrer" href="https://pubs.acs.org/doi/suppl/10.1021/acs.analchem.5c05771/suppl_file/ac5c05771_si_001.pdf">Figures S4 and S5</a> (see the Supporting Information). These data provide evidence that when ion mobility is able to separate out species that overlap in m/z yet differ in mobility, CDMS could be an alternative or used complementarily since the species likely also differ in charge.<img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Anal_Chem_2025_97_49_27057_27063_Figure_3_A_Mass_spectra_of_WT_TTR_and_CT_TTR_homotetramers_with_thyroxine_binding_61b1e98a94.jpg" alt="Anal. Chem. 2025, 97, 49, 27057–27063: Figure 3. (A) Mass spectra of WT-TTR and CT-TTR homotetramers with thyroxine binding. Ligand binding can be resolved, but homotetramers cannot be confidently identified. Charge states labeled are for WT; CT is always one charge state higher at the same m/z range. (B) DMT heatmap resolving WT-TTR and CT-TTR homotetramers with the additional measurement of charge. (C) Deconvoluted mass spectrum from DMT analysis. Both homotetramers and their ligand binding are resolved, and fit for analysis is shown in red. (D) Mole fraction of each homotetramer and their thyroxine binding species. Error bars represent the standard deviation of replicates (n = 3). The significance for each ligand state is in respect to the left adjacent state. *p &lt; 0.05." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Anal_Chem_2025_97_49_27057_27063_Figure_3_A_Mass_spectra_of_WT_TTR_and_CT_TTR_homotetramers_with_thyroxine_binding_61b1e98a94.jpg 181w," sizes="100vw" width="1266" height="1094"><h4>Conclusion</h4>This work demonstrates that quantitatively, Orbitrap-based CDMS/DMT can determine differences in protein-complex stability and ligand binding. By directly comparing WT- with CT-TTR, we show that the CT tag accelerates tetramer dissociation while enhancing T4 binding affinity. These results indicate that the C-terminus, located at the dimer–dimer interface, plays a more significant role in TTR stability and ligand recognition than previously appreciated. <a href="javascript:void(0);">(12)</a> Even the addition of a short peptide sequence perturbs noncovalent interactions at the interface, likely altering solvent accessibility, and the dynamics of the thyroxine binding channel. <a href="javascript:void(0);">(37,54)</a> Such findings underscore the importance of evaluating how seemingly minor sequence modifications, such as affinity tags, can influence fundamental biophysical properties.Beyond TTR, this work positions quantitative CDMS as a broadly applicable strategy for analyzing heterogeneous protein systems, where charge domain separation overcomes the resolution limits of m/z domain measurements in traditional nMS experiments. While much of the field has emphasized extending CDMS to ultrahigh masses and maximal resolution, <a href="javascript:void(0);">(16,18)</a> our results highlight the underexplored potential of applying the method in the 1000–10,000 m/z range common to nMS experiments. This capability is directly relevant to heterogeneous membrane protein complexes, which often display broad distributions of oligomeric states, PTMs, lipid, and ligand adducts that complicate spectra. <a href="javascript:void(0);">(48,55)</a> Similarly, mAbs can oligomerize and exhibit mass heterogeneity from variable glycosylation, affecting pharmacokinetics, effector function, and stability. <a href="javascript:void(0);">(19)</a> CDMS may resolve and quantify such proteoforms in a single experiment without the need for charge reduction or chemical derivatization. <a href="javascript:void(0);">(48,56,57)</a>

Dissecting Heterogeneous Populations of Protein-Complex Samples Using Direct Mass Technology

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<h4>Virtual event: Pharma conclave series 2024</h4>
SCIEX offers the platform for the experts to take the stage and drive technological progress
Welcome to Pharma conclave series 2024 – where our customers’ expertise meets innovation, transforming your knowledge and empowering you to revolutionize your work processes. Join us in discovering techniques that will enhance your skills to accelerate your daily operations in the development of new drugs.
From the research of new markers to the drug development moving into non and GLP bioanalysis. Confidently plan your next lab expansion with the knowledge gained from industry leaders and pioneers.
<ul>
<li>May 16 Event 1: Pharma omics</li>
<li>September 17 Event 2: Impurities</li>
<li>October 23-24 Event 3: Pharma</li>
</ul>
10:00 a.m.–12:00 p.m. CET
<h3>Event 2: Impurities</h3>
Impurities in the pharmaceutical industry. What&#39;s next?
While the regulatory landscape is changing faster than ever, having the right information on impurities available to you at every stage of the drug development is vital to bring safe and effective therapies to the market. Whether you want to optimize your lab’s current capabilities or scale up to meet your future demands, this event creates a platform to share the customer’s expertise.
<blockquote>
Presenter: Bruno Manadas, PhD
</blockquote>
<blockquote>
Dr Manadas is a prominent Cell Biology researcher at CNC-UC since 2017. With expertise in biochemistry, psychology, and biotechnology, he&#39;s authored 130+ publications and led multiple projects. As head of CNC&#39;s Mass Spectrometry node and Associate Editor for Frontiers, he&#39;s contributed significantly to neuroscience and psychiatry. Bruno&#39;s research focuses on cell biology mechanisms, translational biomarker research, and MS-based approaches for studying protein oxidation. He played a pivotal role in establishing the hospital biobank and teaches at the Portuguese Catholic University.
</blockquote>
<blockquote>
Presenter: Dorte Bekker-Jensen
</blockquote>
<blockquote>
Dorte is heading the Applications Development team in Proteomics Research at Evosep. She has long-term experience within proteomics and has focused on technology development by developing simple and automated workflows for high-throughput applications.
</blockquote>
<blockquote>
Presenter: Stefan Ledinger
</blockquote>
<blockquote>
Stefan has 20 years of experience in the life sciences and diagnostic industries. For the last dozen years, he has been serving various functions within biocrates life sciences ag. Most recently, he has been responsible for the market development for metabolomics solutions in the pharmaceutical and biotech industries. He currently serves as Global Head of Sales. Stefan holds degrees in business administration and bioengineering, combining commercial know-how with a passion for science.
</blockquote>
<blockquote>
Presenter: Markus Langsdorf
</blockquote>
<blockquote>
Markus has 14 years of experience in life sciences industries. As he joined biocrates in 2010, he worked for 11 years as application support specialist for biocrates’ metabolomics profiling solutions. 3 years ago he changed his position withing the company to his new function as product manager. Markus studied chemistry and graduated with a PhD in Analytical Chemistry.
</blockquote>

Pharma conclave series 2024: Impurities

<h4>Live from the Lab hosted by NIBRT: Solutions for biotherapeutic analysis</h4>
By your side to accelerate your biopharmaceutical development journey
Join us for the second Live from the Lab of 2024, taking place on September 17th, an exciting biopharma hybrid event featuring the latest advancements in separation technology and mass spectrometry. Focusing on all stages of biotherapeutics development, from discovery and research, all the way to routine and QC analysis, this event will give you valuable insights to accelerate your biopharmaceutical development journey.
The following speakers will talk at this event at the NIBRT research facility which will be streamed live all over Europe:
<ul>
<li>Dr. Jonathan Bones, NIBRT</li>
<li>Prof. Mark Dickman, University of Sheffield</li>
<li>Dr. Dan Bach Kristensen, Symphogen</li>
<li>Dr. Mark Hilliard, Pfizer</li>
<li>Dr. Ken Cook, Thermo Fisher Scientific</li>
<li>Jon Bardsley, Thermo Fisher Scientific</li>
</ul>
Whether you are a researcher, scientist, or industry professional in our region, this biopharma event is not to be missed. Expand your knowledge, network with experts, and discover how the Thermo Scientific solutions will stand by your side to accelerate your biopharmaceutical development journey.
Mark your calendars for September 17th and join us for this impactful hybrid event foster collaboration within the scientific community.
Participation is free of charge, registration is required.
For any questions about this event or regarding your registration, please email CMDEvents@thermofisher.com.
<h4>How to attend?</h4>
3 ways of attending:
<h5>Online attendees (remote):</h5>
Unable to travel to a satellite? Not a problem! You can register to attend conveniently online from your desk or anywhere else through the live stream from NIBRT.
<ul>
<li><a href="https://assets.thermofisher.com/TFS-Assets/CMD/Flyers/fl-003119-pb-live-from-the-lab-ms-biotherapeutics-agenda-online-fl003119-em-en.pdf">Agenda</a></li>
</ul>
<h5>NIBRT Research Facility (in-person event):</h5>
Join the heart of the event where the main agenda will be hosted and live streamed across our region to our satellite events and our online audience. Attendance through VIP invitation only!
<h5>Join a ‘Satellite’ location (in-person event):</h5>
Across Europe, our in-person satellite events will be broadcasting the main agenda live streamed from NIBRT, Dublin and supplement the day with local speakers/lab tours pending on the satellite. Take this opportunity to network with fellow scientists and meet your Thermo Fisher representatives from technical, commercial, and service functions. Check out the closest location to you and their individual agenda!
<ul>
<li>Søborg, Denmark (AGC Biologics)</li>
<li>Dreieich, Germany (Thermo Fisher Scientific)</li>
<li>Segrate, Italy (MI) (Thermo Fisher Scientific S.P.A.)</li>
<li>Leiden, Netherlands (Janssen Biologics BV)</li>
<li>Ljubljana, Slovenia (Four Points by Sheraton Ljubljana Mons)</li>
<li>Reinach, Switzerland (Thermo Fisher Scientific)</li>
<li>Hemel Hempstead, UK (Thermo Fisher Scientific)</li>
</ul>

Thermo Fisher Scientific: Live from the Lab: Solutions for biotherapeutics

Live from the Lab: Solutions for biotherapeutics

An exciting biopharma hybrid event featuring the latest advancements in separation technology and mass spectrometry.

MALDI Top-Down Sequencing (MALDI-TDS) delivers N- and C-terminal sequence readout for intact proteins or protein subunits within minutes.
Leverage the novel neofleX™ MALDI-TDS workflow for verification of therapeutic antibodies, antigens and other protein expression products regarding sequence, terminal status and modifications and, thereby, enable instant decision making in strain development and production in biopharma or other branches of industry.
During this webinar, you will learn:
<ul>
<li>Overview of neofleX technology and its benefits to life sciences applications
</li>
<li>neofleX top-down workflow: Protein sequence verification with shortest time-to-result
</li>
<li>OmniScape™ software: From data to sequencing results with just a few mouse clicks
</li>
</ul>
Who should attend:
<ul>
<li>Characterization team leaders in biopharma companies and CROs
</li>
<li>Mass spectrometry core lab managers
</li>
<li>Researchers who are interested in fast protein characterization methods
</li>
<li>Anyone who needs to deliver instant sequence verification for protein expression products
</li>
<li>Anyone who would like to learn about the latest MALDI-TOF/TOF technology
</li>
</ul>
<blockquote>
Presenter: Jan Hendrik Kobarg, Ph.D. (Product Manager, MALDI-TOF, Bruker Daltonics, Bremen, Germany)
</blockquote>
<blockquote>
Jan Kobarg, Ph.D., is the product manager for the MALDI-TOF instrument portfolio at Bruker. The latest addition to the portfolio is the neofleX™ MALDI-TOF Benchtop instrument.
</blockquote>
<blockquote>
Presenter: Arndt Asperger, Ph.D. (Senior Applications Scientist, Bruker Daltonics, Bremen, Germany)
</blockquote>
<blockquote>
Arndt is analytical chemist by training and got his Ph.D. from Leipzig University. He joined Bruker in 2002 and has been working in various application related positions with a focus on pharma/biopharma.
</blockquote>

Bruker: LabCast: Simple and fast top-down protein characterization using neofleX benchtop MALDI-TOF/TOF

LabCast: Simple and fast top-down protein characterization using neofleX benchtop MALDI-TOF/TOF

Overview of neofleX technology and its benefits to life sciences applications.

LabCast: Simple and fast top-down protein characterization

The sample throughput of high-sensitivity liquid chromatography-mass spectrometry (LC-MS) based proteomics analysis is restricted by the pressure limitations of LC and column hardware, with some methods wasting as much as 50% of the method cycle time outside of MS acquisition.
Traditional routes for boosting throughput include shorter columns and gradients, higher flow rates, and using trap-and-elute workflows – all of which introduce trade-offs in sensitivity and proteome depth.
The Thermo Scientific™ Vanquish™ Neo tandem direct injection workflow leverages hardware innovations and software intelligence to eliminate method overhead while maintaining data quality across nano, capillary, and micro-flow LC-MS.
In this webinar experts from Evotec and Bayer will showcase how they implemented the tandem direct injection workflow for their proteomics work. Dr. Nagarjuna Nagaraj will present on how Evotec is using tandem direct injection with nano-LC columns packed in-house to boost sample throughput of their chemoproteomics screening pipeline, while Dr. Alexander Boychenko from Bayer will discuss how his lab is using a micro-flow tandem direct injection approach to analyze plasma samples for translational proteomics.
Learning Objectives
<ul>
<li>See how minimizing LC-MS method overhead can accelerate sample throughput while maintaining proteome depth
</li>
<li>Discover how labs are using tandem direct injection for chemoproteomics and translational proteomics applications
</li>
<li>Learn how to implement tandem direct injection in your proteomics workflow
</li>
</ul>
<blockquote>
Presenter: Dr. Nagarjuna Nagaraj (Group Leader High Sensitivity Proteomics, Evotec)
</blockquote>
<blockquote>
Dr. Nagarjuna Nagaraj is a proteomics group leader at Evotec focusing on high sensitivity and high throughput proteomics applications with more than 18 years of experience in proteomics and mass spectrometry. Nagarjuna obtained his PhD from the Matthias Mann lab focusing on sample preparation and MS data acquisition strategies for clinical applications. After a postdoc he directed the mass spectrometry core facility at the Max-Planck-Institute of Biochemistry working on a wide range of protein and peptide mass spectrometry projects such as bottom-up proteomics, structural mass spectrometry including HDX, native and crosslinking MS, intact protein and small molecule analysis.
</blockquote>
<blockquote>
Presenter: Dr. Alexander Boychenko (Head of R&amp;D Laboratory Precision Medicine Markers, Bayer)
</blockquote>
<blockquote>
Alexander Boychenko is leading Omics Research within the Translational Sciences team of the Pharmaceuticals division at Bayer Group. He is focused on implementing innovative analytical technologies and developing novel methodologies for proteomics, metabolomics, and ligand-binding assays (LBA) to comprehensively characterize human and animal model phenotypes, enabling disease understanding and novel biomarker discovery. He holds a Ph.D. in Analytical Chemistry from Kharkiv National University, Ukraine. During his post-doctoral work at the University of Groningen, The Netherlands, he combined LC-MS and immunoassays to discover and validate protein biomarkers in biofluids for cancer screening. Before joining Research and Early Development at Bayer AG, he developed and introduced new liquid chromatography-mass spectrometry products at Thermo Fisher Scientific. Alexander is a subject matter expert in LC-MS, biological data interpretation, and precision medicine. He utilizes his passion for applying technical expertise to develop new medicines.
</blockquote>
<blockquote>
Presenter: Dr. Alec Valenta (Product Marketing Manager, Thermo Fisher Scientific)
</blockquote>
<blockquote>
Alec is the product marketing manager for the Vanquish Neo UHPLC system and low-flow LC columns. Prior to joining Thermo Fisher Scientific he earned his PhD in analytical chemistry from the University of Michigan where he helped advance microdialysis sampling of neurochemicals and developed LC-MS methods to quantify small molecule neurotransmitters, neuropeptides, and metabolites in a variety of complex biological samples.
</blockquote>

Thermo Scientific: A Tandem LC Workflow for Maximizing Mass Spectrometer Utilization in Proteomics

A Tandem LC Workflow for Maximizing Mass Spectrometer Utilization in Proteomics

How experts from Evotec and Bayer implemented the tandem direct injection workflow for their proteomics work.

A Tandem LC Workflow for Maximizing Mass Spectrometer

Today, tomorrow, and forever – PFAS, the “forever chemical”, have emerged as one of the greatest public health concerns of our time, infiltrating our environment and food supply. Herein, join us on Digital Classrooms for an in-depth exploration of PFAS, tracing their origins in everyday consumer products and examining their potential health implications.Our experts, Dr Yap Siew Qi and Elton Goh, will guide you through this critical topic, exploring the regulatory landscape with insights into EFSA and FDA guidelines. They will also introduce Shimadzu's innovative LC-MS/MS solutions, featuring the latest LCMS-TQ RX series. Discover how their robustness, sensitivity, and accuracy can enable effective detection of PFAS in complex food and environmental samples.Let’s work together to overcome global PFAS challenges. Register now to stay informed on this pressing issue!<h5>Learning Objectives:</h5><ul><li>Understand the implications of PFAS presence in food and environment &nbsp;</li><li>Navigate the evolving regulatory landscape for PFAS &nbsp;</li><li>Discover the capabilities of Shimadzu LCMS-TQ RX to detect PFAS in food and environmental samples</li></ul><blockquote>Presenter: Goh Wan Yu Elton (Market Specialist Food, Shimadzu (Asia Pacific) Pte Ltd)Elton is a dedicated and experienced Food Market Specialist at Shimadzu, bringing over nine years of expertise in food safety and analytical chemistry to his role. His career began at the Singapore Food Regulatory Board, where he spent seven years honing his skills and developing a profound understanding of regulatory standards and testing protocols. With a passion for knowledge and innovation, Elton transitioned to the private sector, contributing significantly to the advancement of analytical techniques and the development of novel food safety solutions during his two-year tenure at a research and development centre.Now at Shimadzu, Elton continues to excel, leveraging his expertise to develop cutting-edge analytical methods for detecting and quantifying various compounds in food products. His commitment to food science is evident in his efforts to enhance safety standards, build consumer trust, and make meaningful contributions to the global food industry.Presenter:&nbsp;Yap Siew Qi (LCMS Product Specialist, Shimadzu (Asia Pacific) Pte Ltd)Yap Siew Qi is an experienced professional in analytical chemistry, with a strong focus on liquid chromatography-mass spectrometry (LCMS). Her career commenced as a Research Assistant at the NUS Life Sciences Institute, where she acquired substantial experience in scientific research and laboratory techniques. This role was pivotal in developing her comprehensive understanding of life sciences, setting the stage for her future contributions to analytical chemistry and instrumentation.In 2022, her journey continued at Shimadzu (Asia Pacific), where she joined as an Application Chemist, focusing on LC/LCMS. In this capacity, she refined her expertise in LCMS technology, delivering prompt application support and technical solutions for the customers. Her exceptional skills and knowledge in LCMS soon led to her advancement to the position of Product Specialist. In this role, she continues to provide specialized knowledge and support, demonstrating her dedication to the progression of analytical chemistry and her proficiency in applying innovative technology within the field.Presenter:&nbsp;Mr. Ramaswamy Parthiban (Assistant Vice President, TUV SUD South Asia)Mr. R Parthiban has a robust background in operations management and quality assurance within the testing, inspection, and certification industry. He currently serves as AVP at TUV SUD South Asia, a position he has held since March 2022. Prior to this, he was the National Head at Prime Certification &amp; Inspection from April 2020 to June 2021. His experience also includes a significant role as Director of Operations at ALS Testing Services India Pvt Ltd from July 2018 to March 2020, where he led strategic initiatives and operational excellence.Earlier in his career, Mr. Parthiban held leadership roles including VP Operations at Merieux NutriSciences and Laboratory Manager for Chemistry at Exova Bahrain/KSA. His extensive experience in the industry is complemented by his earlier positions as Senior Manager at Coca-Cola and Laboratory Manager at SGS India Private Ltd. He began his career as a Research Associate at the John F. Welch Technology Center and ITC R&amp;D Center, eventually progressing to an Executive role in Quality Assurance at Sanmar Speciality Chemicals Ltd. Mr. Parthiban's career is marked by his expertise in quality systems, operational management, and strategic leadership in both national and international contexts.</blockquote>

Shimadzu: Discover The Latest LC-MS/MS Innovations For PFAS Challenges

Discover The Latest LC-MS/MS Innovations For PFAS Challenges

Discover how their robustness, sensitivity, and accuracy can enable effective detection of PFAS in complex food and environmental samples.

Per- and Polyfluoroalkyl Substances (PFAS) are exceptionally versatile chemicals and have seemingly endless applications that include more than 12,000 different compounds.Due to the persistent nature of these compounds, as well as their ability to be easily transported through the environment, there is a significant concern for the presence of PFAS in the human body. A LC-MS/MS method utilizing microelution SPE for sample cleanup, presented for analysis of seven PFAS, identified significant by National Academies of Sciences, Engineering, and Medicine for clinical research and biomonitoring in public health labs.&nbsp;<h4>Who Should Attend:</h4><ul><li>Any scientists interested in LC-MS/MS and sample preparation or anyone curious about PFAS from biological samples</li></ul><h4>Key Learning Points:</h4><ul><li>Learn key steps to LC-MS/MS and sample preparation of biological samples</li><li>Understand potential Challenges when extracting PFAS from biological samples</li><li>Find advantages of using Micro elution SPE sorbent technology</li></ul><blockquote>Presenter: Shahana W. Huq (Senior Application Scientist, Phenomenex)Shahana is a Senior Application Scientist focusing on emerging technologies and solutions within Clinical Research and Sample Preparation, at the Phenomenex Applications Laboratory since the year 2000. She holds an MS in Biochemistry from California State University, Los Angeles. Her work involves experimental design, analytical method development, optimization, and troubleshooting. &nbsp;Furthermore, she has extensive expertise in R&amp;D product innovation and application development, as well as hands-on experience in sample preparation and LC-MS/MS. Shahana’s drive, and vision of the clinical field has motivated her to participate in various scientific publications and even as a coauthor of Phenomenex patent on sample preparation products. Currently, Shahana works closely with global customers in the applied market, providing technical support and consultation services as well as onsite seminars and webinars to promote peer development. &nbsp;Presenter: Richard Jack, PhD (Global Market Development Manager, Phenomenex)Richard is the global market development manager for the environmental and food markets at Phenomenex. He has over 18 years of experience with chromatography and mass spectrometry for the environmental, food, cannabis, semiconductor, chemical, and pharmaceutical industries. Jack has collaborated with global regulatory agencies to develop validated methods through new applications, instrumentation, column chemistries, and software. He is a former EPA scientific advisor for the EPA’s panel on hydraulic fracturing and a coauthor for EPA 557 and 557.1, ASTM D8001, and updates to D4327 and D6919 methods. He is currently the second vice chairman for the ASTM D19 subcommittee on water analysis. Previously, Jack was a vertical marketing manager with Thermo Fisher Scientific, Dionex, and Hitachi High Technologies, where he designed analytical instrumentation, including IC and HPLC systems, pumps, autosamplers, and a variety of detectors. He received his PhD in biochemistry and anaerobic microbiology from Virginia Tech in Blacksburg, Virginia. He received his master’s degree in ecology from the University of Tennessee in Knoxville.</blockquote>

Phenomenex: LC-MS/MS Analysis of PFAS in Bovine Serum for Clinical Research Using Microelution SPE

LC-MS/MS Analysis of PFAS in Bovine Serum for Clinical Research Using Microelution SPE

A LC-MS/MS method utilizing microelution SPE for sample cleanup, presented for analysis of seven PFAS.

LC-MS/MS Analysis of PFAS in Bovine Serum

Understanding disease at a biological level requires deep, precise analysis. Multi-omics assays provide this depth, offering critical insights into human health and disease progression. However, these advanced methods come with challenges—especially when it comes to standardization and reproducibility.<h4>Join us for an informative webinar and panel discussion where we will:</h4><ul><li>Explore Quantitative Metabolomics: Discover how this powerful approach distinguishes health from disease and unlocks new insights into biological mechanisms.&nbsp;</li><li>Learn About Automated Sample Preparation: Get up to speed on the latest advancements in automated workflows, including the use of kit-based LC-MS/MS for reliable metabolomics profiling.Enhance Your Research Efficiency: Understand how integrating advanced technologies can streamline the verification and monitoring of potential biomarkers, leading to more cohesive analytical workflows.</li></ul><h4>Featured Presentations</h4><ul><li>Hear from experts at the University of Minnesota and University of Surrey as they showcase real-world applications and validations of these workflows. You'll gain exclusive insights from leaders in the field who are at the forefront of metabolomics research.</li></ul><h4>Key Learning Points</h4><ul><li>Unlock the Power of Quantitative Metabolomics: Learn how this approach helps differentiate health from disease and provides crucial insights into biological processes.</li><li>Streamline Your Research: Discover how comprehensive analytical workflows can speed up the verification and monitoring of lipid and small molecule biomarkers.</li><li>Dive into Advanced Automation: Explore cutting-edge sample preparation with the Waters™ Andrew+ automated liquid handler and the biocrates MxP® Quant 500 kit, transforming targeted metabolomics studies.</li><li>Master LC-MS/MS Workflows: Gain insights into the expanded biocrates MxP® Quant 500 XL, optimized for use with the Waters Xevo™ TQ Absolute MS, for robust lipidomics and metabolomics data.</li><li>Engage with Leading Experts: Take advantage of the opportunity to interact with experts from the University of Minnesota, University of Surrey, Waters Corporation, and Biocrates.</li></ul><blockquote>Presenter: Dr Holly-May Lewis (Senior Laboratory Technician, University of Surrey, UK)Dr. Holly-May Lewis is a Senior Laboratory Technician and specialist in Liquid Chromatography Mass Spectrometry (UPLC-MS/MS) at the University of Surrey's Chronobiology Section. With a PhD in Chemistry from the University of Surrey, she brings deep expertise in analytical chemistry to her current work, where she focuses on cutting-edge UPLC-MS/MS metabolomics of human samples.Presenter:&nbsp;Professor Debra Skene PhD (Professor of Neuroendocrinology, University of Surrey, UK)Debra J. Skene, PhD, FMedSci is a distinguished Professor of Neuroendocrinology in the Chronobiology Section at the University of Surrey, UK. With over 30 years of research experience and more than 200 peer-reviewed publications, her work focuses on the human circadian timing system and related sleep/wake disorders, particularly in blind individuals, shift workers, and older adults. Currently, her research explores the links between circadian clocks, sleep, and metabolism in both health and disease, utilizing advanced metabolomics techniquesPresenter:&nbsp;Dr Zachary J Mayer (Research Scientist at the Center for Metabolomics and Proteomics (CMSP), University of Minnesota)Dr Zachary Mayer is a Research Scientist at the Center for Metabolomics and Proteomics (CMSP) at the University of Minnesota, specializing in high-throughput targeted metabolomics by Liquid Chromatography Mass Spectrometry (LC-MS), sample preparation, and laboratory automation.</blockquote>

Waters Corporation: LC-MS/MS Workflows for Kit Based Quantitative Metabolic Profiling

LC-MS/MS Workflows for Kit Based Quantitative Metabolic Profiling

Multi-omics assays provide this depth, offering critical insights into human health and disease progression.

LC-MS/MS Workflows for Kit Based Quantitative Metabolic

The presence of per- and polyfluoroalkyl substances (PFAS) in food supply chains is a growing concern due to their environmental persistence and potential health risks.As awareness increases, the demand for effective regulations and advanced analytical methods for detecting PFAS in food is critical. In this webinar hosted by Agilent in association with AOAC India Section, we will explore the current PFAS contamination landscape, regulatory frameworks, and the latest analytical solutions.The session will cover PFAS characteristics and the challenges they present to food safety. We will examine global and regional regulations, with a focus on evolving standards in India and the South Asia Pacific region. Also, the webinar will highlight advanced analytical approaches for different food matrices like seafood, eggs, meat, infant formula, packaging material, including sample preparation, instrumentation, and reporting essential for effective PFAS detection. Agilent's robust LC/MS and GC/MS PFAS testing solutions will be showcased along with the Captiva EMR sample preparation kit, demonstrating how they can help food manufacturers, exporters, and regulatory bodies ensure compliance with global standards.<h4>Learning outcomes:</h4><ul><li>Comprehensive understanding of PFAS characteristics and the regulatory landscape across global and regional markets, including India and the South Asia Pacific.</li><li>Advanced analytical techniques for PFAS detection in food, covering sample preparation, instrumentation, and reporting methodologies.</li><li>Practical insights into implementing PFAS testing solutions to ensure compliance with international food safety standards.</li></ul><h4>Who should attend:</h4><ul><li>Food scientists, analytical chemists, regulatory authorities, government lab personnel, industry professionals in food manufacturing, packaging, exporting, R&amp;D scientists, toxicologists, QA/QC analysts and managers, academicians, and researchers focused on PFAS analysis and food safety.</li></ul><blockquote>Presenter: Dr. Nick Birse (Lecturer, School of Biological Sciences, Queen's University, Belfast, Northern Ireland, UK)Nick Birse is a Lecturer in Mass Spectrometry at Queen’s University Belfast, where he focuses on the use of mass spectrometry to solve key food integrity challenges, such as the measurement of chemical contaminants and natural toxins, fingerprinting foods to determine geographic origin and help detect fraud, or simply to measure key quality, flavour and taste attributes, all of which makes foods tastier, healthier and safer for consumers. Nick previously served as a Research Fellow within Prof Chris Elliott’s research group, assisting in the execution of Chris’s 2021 Agilent Thought Leader Award. He holds a PhD in Analytical Chemistry from Queen’s University Belfast.Presenter:&nbsp;Dr. Niladri Sekhar Chatterjee (Senior Scientist and DST Inspire Fellow, ICAR-Central Institute of Fisheries Technology, India)Dr. Niladri has more than 10 years of experience in precision measurement of small molecules in food, biological, and environmental matrices. His doctoral research is on pesticide metabolism, and environmental fate in crop, soil, and water. His postdoctoral research is on seafood authentication using high resolution mass spectrometry-based metabolomics and molecular spectroscopy fingerprinting. He currently works as a Senior Scientist in the Biochemistry and Nutrition Division at ICAR Central Institute of Fisheries in Kochi. At CIFT, he has been instrumental in establishing a state-of-the-art analytical chemistry laboratory from scratch which is today one of the National Reference Laboratories. He has received external grants from the Science and Engineering Research Board (SERB) India, National Agricultural Science Fund, and FSSAI. In the NRL, he has developed and validated precision measurement methods for multiclass analysis of pesticides, antibiotic residues, and endocrine disruptors and is actively managing the ISO 17025 accreditation of the laboratory. His research work has resulted in several peer reviewed publications.Presenter:&nbsp;Dr. Lorna De-Leoz (Global Food Segment Director, Agilent Technologies, USA)Lorna De Leoz has nearly 20 years of experience in the food industry. As the Global Food Segment Director, she collaborates with scientists to help develop accurate and reliable testing for food safety, quality, and authenticity. Lorna worked at the National Institute of Standards and Technology in the Mass Spectrometry Data Center for seven years. She completed her Ph.D. in Chemistry at University of California, Davis on milk oligosaccharides and glycan biomarkers analyses.</blockquote>

Agilent Technologies: PFAS in Food Supply Chains: Regulations and Analytical Approaches: in association with AOAC India Section

PFAS in Food Supply Chains: Regulations and Analytical Approaches: in association with AOAC India Section

PFAS characteristics and the challenges they present to food safety.

PFAS in Food Supply Chains: Regulations and Analytical

<h4>The Waters 2024 Virtual Summer School - An Educational Journey to Master Food &amp; Environmental Analysis</h4>Unlock the full potential of LC-MS techniques with our immersive and educational sessions at the Waters Virtual F&amp;E Late Summer School 2024. From September 24th to 26th, engage with leading experts and discover cutting-edge methods for environmental and food analysis.<h5>Why attend?</h5><ul><li>Comprehensive Learning: Dive deep into advanced LC-MS techniques and gain practical knowledge to enhance your laboratory's analytical capabilities.</li><li>Expert Insights: Learn from industry leaders and gain firsthand experience through detailed modules on chromatography, mass spectrometry, and sample preparation.</li><li>Regulatory Compliance: Stay ahead with the latest insights on EU regulations for PFAS analysis in food and water, ensuring your lab meets stringent standards.</li></ul><h3>Episode #2: Tandem Quadrupole MS and MS-Method Development | F&amp;E Summer School - Day 1 - Module 2</h3>In this episode it’s all about the tandem quadrupole mass specs and the setup of MS-methods. We will demonstrate, using actual hardware, how simple it is to prevent contamination or clean a system, regardless of whether it’s at the low or high end of the sensitivity spectrum. You will learn valuable tips and tricks about UniSpray, a unique ion source and alternative to ESI.With our new waters_connect for Quantitation software, we will guide you through the different stages of MS-method development. You will learn how straightforward automated compound optimization can be, and how intuitively the new Method Acquisition Editor can be utilized by operators providing a clear structured all in one view of compounds and parameters. Search options make it easy to navigate through large multi-residue methods. And in the end, you are just one click away from the processing method that will be the topic of Episode 4.<h5>What you'll learn:</h5><ul><li>Easy operation and maintenance of Waters TQ-MS systems</li><li>Usage and benefits of UniSpray ion source</li><li>Step by step MS-method development with waters_connect for Quantitation</li></ul><blockquote>Presenter:&nbsp;Benjamin Wuyts, PhD (Senior Solution Support Specialist Europe, Waters Corporation)Benjamin initially joined Waters as an application chemist working in the Belgian demo lab, performing liquid chromatography-mass spectrometry demonstrations, support and training. In 2016 Benjamin became a European application chemist focusing on projects to support emerging applications. Benjamin has been focused over several years in establishing the Waters solution to the analysis of polar anionic pesticides, utilising novel column phases and LC-MS/MS technologies.</blockquote>

Waters Corporation: The Waters 2024 Virtual Summer School

Tandem Quadrupole MS and MS-Method Development

In this episode it’s all about the tandem quadrupole mass specs and the setup of MS-methods.

PFAS testing has become one of the most important topics facing food and environmental laboratories over the last two years.&nbsp;Phenomenex and SCIEX have worked with collaborators to overcome the significant challenges of such analyses, and now offer extremely sensitive and robust LC-MS/MS and sample preparation solutions, with methods and software which are ready to implement.<h4>Key Learning Points</h4><ul><li>Background to PFAS and their regulations</li><li>System Settings and requirements</li><li>Correct HPLC column choice</li><li>Sample Preparation strategies from various matrices</li><li>PFAS as ubiquitous contaminants - Inerting the system</li><li>A sensitive and robust method applicable to water and food sample</li><li>Some insight about designed for PFAS products</li></ul><h4>Who should attend</h4><ul><li>LC/MS or Sample Prep Users, working with several matrices that want to get tips on PFAS method development or on improving their method.</li></ul><blockquote>Presenter: Jianru Stahl-Zeng, Dr.&nbsp; (Senior Technical Marketing Manager EMEAI, SCIEX)Jianru has been part of SCIEX since 2001. She initially was part of our application Support team and covered not only proteomics but also small molecules applications. Jianru moved to technical marketing and focused on Food and Environmental applications.Since 2018, she is managing the EMEAI technical marketing team and projects. Jianru is also the Global Technical Leader for Food and &nbsp;Environmental application.Presenter:&nbsp;Duilio Romanello (Senior Technical Specialist, Phenomenex)Duilio has been working with Phenomenex since 2008. Since 2010 he has been Product Specialist for the GC and SPE lines and then Account Manager for the South of Italy. Since November 2023 he is covering the role of Senior Technical Specialist.He has acquired experience in the Food and Environmental sectors by collaborating with important companies in the industry and providing technical support for the development of method optimization, guidance on applications and troubleshooting, assistance in choosing columns for HPLC, Gas Chromatography (GC), SFC and SPE.</blockquote>

Phenomenex: Advanced strategies in LC/MS/MS for the clean-up and the analysis of per- and polyfluoroalkyl substances (PFAS) in various matrices

Advanced strategies in LC/MS/MS for the clean-up and the analysis of per- and polyfluoroalkyl substances (PFAS) in various matrices

Extremely sensitive and robust LC-MS/MS and sample preparation solutions, with methods and software which are ready to implement.

LCMS webinars focusing on LC/MS/MS - page 34