<p><strong>Professor Michaela Rumlová, head of a research group of the National Institute of Virology and Bacteriology at UCT Prague (and Vice-Rector for doctoral studies), holder of a licensed patent &nbsp;for antiretroviral testing assays, and recipient of the Czech Science Foundation’s President’s Award for HIV research, has also become the principal investigator of the bilateral INTER-EXCELLENCE II project “</strong><i><strong>Cellular factors regulating the export and packaging of unspliced ​​retroviral RNA</strong></i><strong>”, starting this year. In this interview, she explains how retroviruses such as HIV “hack” the cell in order to export their own genome from the nucleus into the cytoplasm, and then to the site where it is specifically packaged into nascent viral particles, a key step in viral replication. Her team is collaborating with the NIH (National Institutes of Health) and Rockefeller University to elucidate mechanisms that may help reveal new vulnerable points in the retrovirus replication cycle while simultaneously clarifying related cellular processes.</strong></p><h5><strong>Could you describe your project and its main objectives?</strong></h5><p>Retroviruses, such as HIV, are viruses that carry genetic information in the form of RNA; however, after entering a cell, they are able to reverse transcribe it into DNA and integrate it into the host genome. In order to replicate, they must transport their genetic information—in the form of unspliced RNA—from the nucleus into the cytosol. There, it serves both as a template for the production of viral proteins and as the genome of new viral particles. This is highly unusual, as the cell very carefully controls which RNA molecules are allowed to leave the nucleus. Normal protein-coding cellular RNAs, known as messenger RNAs (mRNAs), must undergo splicing—a processing step in which certain segments are removed—before being exported. When this process fails, mRNA that retains these segments is held back in the nucleus as part of quality control and subsequently degraded. Retroviruses have managed to outwit this cellular control machinery, allowing their unspliced RNA to pass into the cytoplasm.</p><p>Our project focuses on identifying the cellular proteins that assist retroviruses in this step and on the mechanism by which protein complexes bound to viral RNA are remodelled during RNA transport. The goal is to understand how the virus uses cellular mechanisms to its advantage and how this pathway is linked to the subsequent fate of the RNA, i.e. to its translation and packaging into new viral particles.</p><h5><strong>Why is this export of unspliced ​​RNA so important for retroviral replication?</strong></h5><p>Because this RNA has a dual role for the virus. On the one hand, it serves as a template for synthesizing viral proteins in the cytoplasm; on the other, it represents the genetic material itself, which must be packaged into new viral particles. The virus must package its RNA genome in its unspliced form, as only this RNA contains essential sequences without which further viral replication would be impossible.</p><p><strong>Simply put: </strong>the virus needs to get a complete copy of its genetic instructions out of the nucleus. Once the viral RNA is in the cytoplasm, it can be used as a template for synthesizing viral proteins; and a portion of these RNA molecules is then selected and packaged into a new particle as a genome. We are interested in how the virus forces the cell to allow viral RNA that is, from the cell’s perspective, “unfinished,” that is unspliced, to leave the nucleus. We are also investigating how the cell and the virus together - albeit unintentionally from the cell’s perspective - determine which RNA molecule will be translated into proteins and which will be packaged as the genome. This is one of the key, yet still not fully understood, steps of retroviral replication.</p><h5><strong>What do you think it was about your project that impressed the Ministry of Education, Youth and Sports’ jury the most?</strong></h5><p>I believe the decisive factor was a combination of several elements. First, the reviewers were primarily interested in the scientific problem itself. We are investigating a mechanism that is essential for retroviruses, but still not sufficiently understood, which may also have broader implications for the regulation of cellular processes. In addition, the quality of the team, our strong collaboration with the<strong> NIH </strong>and <strong>Rockefeller University</strong>, and the use of modern methods that will allow us to track viral RNA in great detail also played a role. It was also important that the project has clear objectives and a realistic plan.</p><h5><strong>How will this research contribute to the development of new treatments for retroviral infections?</strong></h5><p>It is important to say that this is primarily basic research. Rather than developing a new drug directly, we are trying to understand, at the molecular level, the step that the virus cannot do without. Only when we fully understand the mechanism of these cellular processes and identify the essential factors for the export of unspliced ​​RNA and how they function, will we be able to better determine which interactions or mechanisms represent potentially vulnerable points. Similar mechanisms associated with the export of unspliced ​​mRNA also appear in some diseases, such as cancer, so I &nbsp;see the benefit of our research mainly in that it will refine our understanding of these pathological processes in the cell, and retroviruses help us do so. Therefore, our work on retroviruses not only shows us how the virus works, but also how the cell itself works.</p><h5><strong>What responsibilities does your UCT Prague team have? How does the collaboration with your American partners work?</strong></h5><p>At UCT Prague, we are conducting the project’s primary experimental work. Our research involves cell cultures, viral models, and various biochemical techniques ranging from immunochemistry to microscopy that allow us to track viral RNA and identify its cellular partners. In collaboration with colleagues from <strong>NIH, Frederick</strong>, we are developing single-particle tracking methods to observe viral RNA movement in living cells across both space and time. Furthermore, we are working with colleagues from <strong>Rockefeller University to employ iCLIP </strong>methods for the precise mapping of protein-binding sites on RNA. Both collaborations are valuable to us, because they are not just about data sharing, but also about the transfer of expertise and detailed know-how.</p><h5><strong>Who else is collaborating on the project?</strong></h5><p>At UCT Prague, we collaborate closely with the Department of Biotechnology and the Department of Biochemistry and Microbiology. Externally, we work with colleagues from the Institute of<strong> Organic Chemistry and Biochemistry of the CAS (IOCB Prague)</strong> and the <strong>Central European Institute of Technology in Brno (CEITEC)</strong>. Our research naturally necessitates the integration of diverse fields of expertise; it requires combining virology, cell biology, and biochemistry with advanced imaging and modern approaches to study RNA-protein interactions. This is exactly what makes our project capable of addressing complex questions that a single laboratory cannot tackle on its own.</p><h5><strong>Are there plans to continue the project? Have you brought on new collaborators, and are you looking to welcome new colleagues to the team?</strong></h5><p>Yes, definitely. In basic research, it often happens that one answered question opens up several more. High-quality, properly interpreted data provide a foundation you can build on — and that is what moves scientists forward. It is already clear that the topic of retroviral RNA export and packaging has broader implications and may reveal more general principles of how RNA functions within the cell. We also hope the project will be attractive to students, as it connects classical molecular biology, biochemistry, and virology with cutting-edge biotechnological methods. Students are already involved in the project as part of their bachelor’s and master’s theses.</p><h5><strong>What was the personal turning point that motivated you to pursue a long-term investigation into retroviral particles?</strong></h5><p>I first encountered retroviruses in the early 1990s during my diploma thesis, when I was working at IOCB Prague on HIV-1 protease inhibitors. At that time, AIDS could not yet be effectively treated, and retrovirus research had a completely different dimension. From the very beginning, I was fascinated by how such a relatively small and simple virus can outsmart evolutionarily highly optimized cellular processes and turn them to its own advantage. And that has never stopped fascinating me. From a scientific point of view, retroviruses are remarkable; just consider one of their enzymes, reverse transcriptase, without which no diagnostic or research laboratory in the world could function today. Without it, it would be impossible to diagnose RNA viruses like SARS-CoV-2, influenza, or HIV using RT-PCR tests, or, for example to produce recombinant human proteins in bacterial cells.</p>

Tracking unspliced RNA: Professor Rumlová studies how retroviruses “hack” cells

News from LabRulezLCMS Library - Week 20, 2026

<p><strong>🎤Lindsay Repka&nbsp;</strong></p><p><strong>Lindsay Repka is a chemistry professor at Middlebury College whose research explores photoredox chemistry, catalyst stability, and visible-light-driven transformations. Her lab emphasizes both mechanistic insight and hands-on student training in advanced analytical instrumentation.</strong></p><p>In this episode of Concentrating on Chromatography, we sit down with Lindsay Repka to discuss how LC-MS and GC-MS transformed her lab’s approach to photoredox chemistry.</p><p>What began as a project to develop a visible-light photocrosslinking handle unexpectedly led to a major discovery: the solvent (DMF) was reacting with the photocatalyst itself. Using high-resolution LC-MS, Lindsay’s team observed multiple solvent adducts forming — sometimes with complete catalyst consumption. That discovery reshaped their research direction.</p><p>This episode is a rare deep dive into both LC-MS and GC-MS within the same research project, showing how chromatography-driven insight can turn unexpected degradation into productive new reactivity.</p><p><strong>If you work in:</strong></p><ul><li>Photoredox chemistry</li><li>Reaction optimization</li><li>Mass spectrometry method development</li><li>Catalyst screening</li><li>Academic synthetic chemistry</li></ul><p>…this conversation will resonate.</p><h4><strong>Key Topics Covered</strong></h4><ul><li>Photocatalyst stability in DMF, DCE, and MeCN</li><li>Demethylation under mild visible-light conditions</li><li>High-resolution Q-TOF LC-MS quantitation</li><li>Internal standard methodology in GC-MS</li><li>Signal-to-noise improvement using extracted ion chromatograms</li><li>Reaction reproducibility and quality control strategy</li></ul><h3><strong>Video Transcription</strong></h3><p>In this interview, Lindsay Repka discusses the role of photoredox chemistry in method development, photocrosslinking, solvent activation, catalyst stability, and the use of LC-MS and GC-MS to understand reaction pathways.</p><h4><strong>What is photoredox chemistry?</strong></h4><p>Photoredox chemistry uses light to activate a photocatalyst, which can either donate or accept an electron from a substrate. This generates radical species that can undergo new types of reactivity.</p><p>Repka explains that one of the key advantages of photoredox chemistry is its ability to operate under mild conditions, often using visible light. This makes it attractive for sensitive molecules and biological systems, including potential applications in drug release, live-cell chemistry, and drug development.</p><h4><strong>Photocrosslinking and drug-target interactions</strong></h4><p>Repka’s group initially aimed to develop visible-light photocrosslinking methods to study drug interactions with biological targets. Photocrosslinking can covalently link a drug to its target, making it easier to identify the biological target of a compound.</p><p>Traditional photocrosslinking often uses UV light, but visible light offers milder and more biologically compatible conditions.</p><h4><strong>From photocrosslinking to solvent activation</strong></h4><p>The project shifted direction when the desired photocrosslinking reactions gave very low yields. Instead of reacting with amino acids as intended, the radical intermediate mainly reacted with itself.</p><p>The team explored different solvents to stabilize the radical, but found that some solvents, such as DMF, were not inert. Instead, the solvent reacted with the photocatalyst or radical species, revealing unexpected solvent activation chemistry.</p><h4><strong>Why LC-MS was critical</strong></h4><p>LC-MS became essential because the reaction mixtures were too complex and low-yielding for NMR or GC-MS. High-resolution LC-MS allowed the team to detect minor products, identify photocatalyst modifications, and observe solvent addition products with high confidence.</p><p>Repka emphasizes that LC-MS was both a qualitative and quantitative tool. It was used quantitatively to measure how much photocatalyst remained after light exposure, and qualitatively to identify degradation products and new reactivity pathways.</p><h4><strong>Photocatalyst stability workflow</strong></h4><p>The team developed a systematic workflow to evaluate photocatalyst stability under light exposure. Because catalyst degradation was time-sensitive, reproducibility required strict control of experimental conditions.</p><p>Key steps included using a glove box, commercial deoxygenated solvents, accurate weighing on a microbalance, controlled light exposure in a photoreactor, and temperature control with a chiller. These precautions helped reduce day-to-day variability and enabled reproducible measurements of catalyst remaining.</p><h4><strong>Quantifying catalyst remaining by LC-MS</strong></h4><p>Calibration curves were prepared using pure photocatalyst solutions and LC-MS ion areas. The team worked in the nanomolar range to maintain linearity.</p><p>Instead of relying only on total ion chromatograms, they used extracted ion chromatograms (EICs) to improve signal-to-noise for the photocatalyst signal. Baseline subtraction was also applied to reduce noise and improve quantitation at low concentrations.</p><p>Calibration curves and quality control samples were prepared and measured on the same day as the reaction samples, because LC-MS signal intensity can vary significantly from day to day.</p><h4><strong>Why GC-MS was used later</strong></h4><p>For demethylation studies, the group moved to GC-MS because the substrates and products were small and volatile enough for gas chromatography. GC-MS was better suited for rapidly screening many reaction conditions, including different photocatalysts, solvents, and oxidants.</p><p>Instead of generating full calibration curves for every experiment, the team used an internal standard to streamline analysis and determine percent conversion based on relative response factors.</p><h4><strong>Practical lessons from the project</strong></h4><p>Repka highlights the importance of careful sample preparation, especially when working with very small quantities of photocatalysts. A high-quality microbalance, stable granite platform, antistatic tools, and aluminum weigh boats improved weighing accuracy and reproducibility.</p><p>She also argues that mass spectrometry should be a standard part of photoredox method development because it can reveal minor products, support reaction optimization, and provide insights that may be difficult or impossible to obtain by NMR alone.</p><h4><strong>Final perspective</strong></h4><p>Beyond the chemistry, Repka emphasizes the importance of student training and mentoring. For her, research is not only about discovering new reactivity but also about giving students practical experience, helping them develop independence, and preparing them for future scientific careers.</p><blockquote><p><strong>This text has been automatically transcribed from a video presentation using AI technology. It may contain inaccuracies and is not guaranteed to be 100% correct.</strong></p></blockquote><blockquote><h4><strong>Concentrating on Chromatography Podcast</strong></h4><p>Dive into the frontiers of chromatography, mass spectrometry, and sample preparation with host David Oliva. Each episode features candid conversations with leading researchers, industry innovators, and passionate scientists who are shaping the future of analytical chemistry. From decoding PFAS detection challenges to exploring the latest in AI-assisted liquid chromatography, this show uncovers practical workflows, sustainability breakthroughs, and the real-world impact of separation science. Whether you’re a chromatographer, lab professional, or researcher you'll discover inspiring content!</p><p>You can find <strong>Concentrating on Chromatography Podcast </strong>in podcast apps:</p><ul><li><a target="_blank" rel="noopener noreferrer" href="https://open.spotify.com/show/1wZg67rCjoGju4yhqxI3tL"><strong>Spotify</strong></a></li><li><a target="_blank" rel="noopener noreferrer" href="https://podcasts.apple.com/tz/podcast/concentrating-on-chromatography/id1788429185"><strong>Apple podcast</strong></a></li><li><a target="_blank" rel="noopener noreferrer" href="https://podscan.fm/podcasts/concentrating-on-chromatography"><strong>podscan</strong></a></li></ul><p>and on <a target="_blank" rel="noopener noreferrer" href="https://www.youtube.com/@ChromatographyTalk/videos"><strong>YouTube channel</strong></a></p></blockquote>

Using LC-MS & GC-MS to Decode Photoredox Catalyst Stability

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Science and research

The University of Chemistry and Technology, Prague is a natural centre of first-rate study and research in the area of chemistry in Czechia and is one of the country's largest educational and research institutions focused on technical chemistry, chemical and biochemical technologies, material and chemical engineering, food chemistry, and environmental studies.

UCT Prague

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LabRulez

Organomation, founded in 1959, designs and manufactures high-quality nitrogen evaporators and extraction systems. Known for innovation and durability, their lab instruments are used globally for efficient sample preparation with strong customer support.

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Waters Corporation

<p><strong>Questions that are answered by structure elucidation and &amp; verification are integral parts of chemical R&amp;D workflows from discovery to development and manufacturing. But as scientists constantly strive to do their work faster and better, they must be careful to ensure that efficiency does not come at the cost of confidence in the determined structure.</strong></p>
<p>Join us as we bring together experts from some of the industry’s leading organizations to share how they leverage software to make the most of their NMR, LC/MS, and GC/MS data and boost their structure elucidation and verification workflows.</p>
<h5>Who won’t want to miss this symposium?</h5>
<ul>
<li><p>Synthetic and medicinal chemists responsible for elucidating and verifying the structures of their reaction products and by-products<br/>Natural products researchers</p>
</li>
<li><p>Members of structure elucidation groups</p>
</li>
<li><p>Decision makers at institutions where chemical structures need to be identified quickly and confidently</p>
</li>
</ul>
<h3><strong>Event Agenda</strong></h3>
<h5><strong>15:30-16:10 (CEST) Novartis CSI: Detectives in the Chemistry Lab</strong></h5>
<ul>
<li><strong>Dorina Kotoni</strong> (Associate Director/Chemical Structure Investigation Group Lead, Novartis)</li>
</ul>
<h5><strong>16:15-16:50 (CEST) Structure Elucidation at Pfizer Sandwich</strong></h5>
<ul>
<li><strong>John Baker</strong> (Principal Scientist, Pfizer)</li>
</ul>
<h5><strong>16:55-17:30 (CEST) A Chemical Characterization Workflow to Speed up Identification of Unknowns</strong></h5>
<ul>
<li><strong>Cindy Roberson</strong> (Principal Scientist, Medtronic)</li>
<li><strong>Joe Buchman</strong> (Senior Chemist, Medtronic)</li>
</ul>


ACD/Labs: What's That Peak? Structure Elucidation & Verification Virtual Symposium 

What's That Peak? Structure Elucidation & Verification Virtual Symposium

What’s in my sample? Did I make what I think I made? What’s that peak?

What's That Peak? Structure Elucidation & Verification

<p><strong>Questions that are answered by structure elucidation and &amp; verification are integral parts of chemical R&amp;D workflows from discovery to development and manufacturing. But as scientists constantly strive to do their work faster and better, they must be careful to ensure that efficiency does not come at the cost of confidence in the determined structure.</strong></p>
<p>Join us as we bring together experts from some of the industry’s leading organizations to share how they leverage software to make the most of their NMR, LC/MS, and GC/MS data and boost their structure elucidation and verification workflows.</p>
<h5>Who won’t want to miss this symposium?</h5>
<ul>
<li><p>Synthetic and medicinal chemists responsible for elucidating and verifying the structures of their reaction products and by-products<br/>Natural products researchers</p>
</li>
<li><p>Members of structure elucidation groups</p>
</li>
<li><p>Decision makers at institutions where chemical structures need to be identified quickly and confidently</p>
</li>
</ul>
<h3><strong>Event Agenda</strong></h3>
<h5><strong>15:30-16:10 (CEST) Combined LC/MS and NMR for Automated Verification of Chemical Structures</strong></h5>
<ul>
<li><strong>Celine Nguyen-Van-Dau</strong> (Head of Integrated Drug Discovery (IDD) Structural Analysis team, Sanofi)</li>
</ul>
<h5><strong>16:15-16:50 (CEST) Pharmaceutical Discovery and Development Analytical Workflows</strong></h5>
<ul>
<li><strong>Kevin Robbins</strong> (Associate Principle Scientist, AstraZeneca)</li>
</ul>
<h5><strong>16:55-17:30 (CEST) Conformational Analysis of Peptidomimetic Drug Leads by NMR</strong></h5>
<ul>
<li><strong>Purnima Khandelwal</strong> (Senior Principal Scientist, Small Molecule Drug Discovery, Bristol Myers Squibb)</li>
</ul>
<h5><strong>Hosted By</strong></h5>
<ul>
<li><strong>Sanji Bhal</strong> (Director, Marketing Communications (ACD/Labs))</li>
<li><strong>Sarah Srokosz</strong> (Marketing Communications Specialist (ACD/Labs))</li>
</ul>


<p><strong>In this educational webinar series, we will go back to the basics of  chromatography, mass spectrometry, data analysis and troubleshooting. Understanding the importance of the basics will enable you to be more effective in your laboratory. During each session we will cover a specific aspect of your LC or LC/MS system:</strong></p>
<ul>
<li>The theory of chromatography and requirements for great separations</li>
<li>The basics of your Liquid Chromatograph (LC): Pumps, injectors, and detectors</li>
<li>An Introduction to mass spectrometry (MS) systems</li>
<li>Considerations with System Suitability</li>
<li>Troubleshooting your LC and MS systems</li>
</ul>
<h3><strong>PART 4  |  INFORMATICS</strong></h3>
<ul>
<li><strong>What is System Suitability and Why is it Important?</strong></li>
<li><strong>Why Should we Prove a System is Suitable for Use?</strong></li>
</ul>
<blockquote>
<p><strong>Presenter:</strong> Lissa Wang (Informatics Business Development Specialist, Waters Corporation)</p>
</blockquote>
<blockquote>
<p>Lissa Aspiras Wang has been with Waters Corporation since 1998 when she started to be a Millennium/Empower Trainer in the Customer Education department. She has also held roles in Technical Service and Global Service and Support for Informatics. Currently, she is part of the Informatics Business Development team supporting the Informatics sales team for Empower and NuGenesis informatics portfolio. Prior to joining Waters, Lissa worked as an analytical chemist in the pharmaceutical, biotechnology and medical device industries for 8 years across the US and the globe.</p>
</blockquote>
<blockquote>
<p><strong>HOST:</strong> Alexandra (Allie) Ahern (Informatics Product Marketing Manager, Americas Field Marketing, Waters Corporation)</p>
</blockquote>
<blockquote>
<p>Allie Ahern has been with Waters for 10 years and is the Informatics Product Marketing Manager within the Americas Field Marketing Organization. Prior to Allie’s current role, she has held other roles at Waters including Service Marketing Product Manager, Implementation Project Manager, and Field Service Engineer.</p>
</blockquote>
<blockquote>
<p><strong>Q&amp;A Panelist:</strong> Neil Lander (Principal Product Manager, Waters Corporation)</p>
</blockquote>
<blockquote>
<p>Neil has worked for Waters Corporation since 1994 and has held a variety of positions including Technical Support Specialist, Technical Trainer, Manager Customer Education and currently, Principal Marketing Manager in Waters in the Informatics group. Neil provides internal support for Empower™ CDS software and is focused on developing laboratory software solutions that help organizations achieve their scientific and operational objectives. Prior to joining Waters Corporation, he was an analytical chemist where he gained experience in both the cosmetics and specialty chemical industries.</p>
</blockquote>
<blockquote>
<p><strong>HOST:</strong> Kieron Faherty, Ph.D. (Separations and Mass Spectrometry Field Marketing Manager, Waters Corporation)</p>
</blockquote>
<blockquote>
<p>Kieron Faherty is the Separations and Mass Spectrometry Field Marketing Manager for the Americas at Waters Corporation. He received his B.Sc. (Hons) in Chemistry from the University of Reading (Reading, Berkshire, UK) and his doctorate in Chemistry from the University of Massachusetts (Amherst, MA). He has held several field-based positions at Waters, most recently as a Field Applications Specialist focused on biopharmaceutical applications using Time-of-Flight mass spectrometry instrumentation.</p>
</blockquote>


Waters Corporation: Fall Back to Basics - Part 4: Informatics

Fall Back to Basics - Part 4: Informatics

PART 4 topics: What is System Suitability and Why is it Important? Why Should we Prove a System is Suitable for Use?

<p><strong>According to the PIC/S guidance complex or open-ended processes that lead to subjective interpretation, can result in an increase in data failures.  One way to reduce this risk is with a simplified, well-defined process.  Empower software enables you with tools to create structure in your chromatographic processes and workflow.  Leveraging a well-defined workflow along with a clear SOP that details important steps, such as how to work thru reprocessing of data, will reduce the risk of failures.  More complexity added to a workflow can result in an increase in data review requirements and subsequently the associated audit trails.</strong></p>
<p>In this workshop we will define a chromatographic workflow with Empower software to support data integrity goals.  Starting with method development, method validation, and routine usage to data acquisition, data processing, reporting results, and using control charts to track and trend data.  </p>
<p>Please join us for this live discussion to learn more and bring your Empower software questions for this exclusive opportunity to ask our expert, Neil Lander, anything about Empower CDS!</p>
<blockquote>
<p><strong>Presenter:</strong> Neil Lander (Principal Product Manager, Waters Corporation)</p>
</blockquote>
<blockquote>
<p>Neil has worked for Waters Corporation since 1994 and has held a variety of positions including Technical Support Specialist, Technical Trainer, Manager Customer Education and currently, Principal Marketing Manager focusing on Informatics solutions. He provides internal support for Empower Chromatography Data System and is focused on developing laboratory software solutions that help organizations achieve their scientific and operational objectives. Prior to joining Waters, he was an analytical chemist where he gained experience in both the cosmetics and specialty chemical industries.</p>
</blockquote>


Waters Corporation: Empower Tips: Ask Me Anything About Creating Efficiency in Your Routine Workflow with Simple Automation Steps

Empower Tips: Ask Me Anything About Creating Efficiency in Your Routine Workflow with Simple Automation Steps

In this workshop we will define a chromatographic workflow with Empower software to support data integrity goals.

Empower Tips: Simple Automation Steps

<p><strong>Event Overview: Pesticide analysis can feel like an uphill battle: an ever-expanding list of target compounds, increasingly stringent regulations that drive lower detection limits, and constantly evolving sample matrices make it challenging to keep up. Agilent&#39;s multi-class, multi-residue methods based on LC/MS and GC/MS, give you a leg up on routine monitoring, enabling high throughput, sensitive detection levels, and rapid quantitative analysis for hundreds of pesticides in a single sample.</strong></p>
<p>Bring all your questions to be answered during our “Ask the Experts” panel. Join Agilent application scientists and engineers who will be available to provide guidance and expertise for all your hardware, software, and application related questions. </p>
<h5>This day will be dedicated to pesticide work using LCMS. Some topics to be discussed are:</h5>
<ul>
<li>Sample clean up in complex matrices: QuEChERS and beyond</li>
<li>Analyte enrichment out of aqueous matrices</li>
<li>New features in MH 12</li>
<li>Tips and tricks for streamlining data review</li>
<li>Facing challenging requirements on selectivity and sensitivity for pesticides analysis</li>
<li>Decision tree for pesticides screening and quantitation</li>
</ul>
<blockquote>
<p><strong>Presenter:</strong> Tina Chambers (Sample Prep Application Scientist, Agilent Technologies, Inc.)</p>
</blockquote>
<blockquote>
<p>Tina started her career in Analytical Chromatography with Varian in 2002 and spent the first five years providing technical support for their LC columns, GC columns and sample preparation portfolios. After three years in sales, she returned to her technical roots with Agilent in 2010, consulting with customers on efficient and optimized sample preparation strategies.</p>
</blockquote>
<blockquote>
<p><strong>Presenter:</strong> Emily Parry, PhD (LC/MS Applications Scientist, Agilent Technologies, Inc.)</p>
</blockquote>
<blockquote>
<p>Emily has been with Agilent for four years. Prior to joining Agilent, Emily developed methods for environmental contaminants in a variety of human and environmental matrices using both targeted and untargeted mass spectrometry techniques. She worked as a researcher at the Environmental Protection Agency (EPA) and Public Health Institute. She received her Ph.D. degree in Environmental Chemistry from the University of California, Davis.</p>
</blockquote>
<blockquote>
<p><strong>Presenter:</strong> Hui Zhao (LCMS Application Scientist, Agilent Technologies, Inc.)</p>
</blockquote>
<blockquote>
<p>Hui has extensive industry experience in developing and validating analytical methods for food/feed nutrition, food safety, dietary supplements and botanicals testing using a variety of analytical techniques and a breadth of sample preparation methodologies. She worked as a Research Scientist at Monsanto, EPL-Bioanalytical Services, Tate &amp; Lyle, Inc. and Lead Staff Scientist at Covance Food Solutions. She holds a Ph.D. in Analytical Chemistry from the University of Missouri. Hui currently works as an LCMS application scientist in Agilent.</p>
</blockquote>


Agilent Technologies: Ask the Experts Panel: Pesticides by LC/MS

Ask the Experts Panel: Pesticides by LC/MS

Join Agilent application scientists who will be available to provide guidance and expertise for all your hardware, software, and application related questions.

<p><strong>Over the last two and a half years, we’ve all had to learn to communicate and work in new ways. With the help of technology, we’ve connected with colleagues across the globe in ways we never thought possible.  The ability to work remotely has ensured continued quality management of products consumed daily. For an analytical chemist or lab manager, quality management may include reviewing and approving data from the safety of your office whether in the lab or at home. No matter where the data is reviewed, completeness, accuracy and traceability must be maintained. This can be accomplished with a secure and efficient software platform.</strong></p>
<p>During this webinar, Kerri-Ann Blake, Product Manager for Titration, and Daniel Schmidt, IT Systems Engineer, will introduce you to the OMNIS data management platform. They will discuss how you can securely implement the software, review and sign data. You’ll learn how to track it all via audit trail from anywhere in the world - giving you peace-of-mind that all your data is being kept safe. </p>
<h5>Key Learning Objectives</h5>
<ul>
<li><strong>Introduce OMNIS software’s seamless networking capability and user management system</strong></li>
<li><strong>Demonstrate convenient audit trail features with next level compliance and data integrity</strong></li>
<li><strong>Discuss system security and ease of implementation</strong></li>
</ul>
<h5>Who Should Attend</h5>
<ul>
<li>QC/QA, R&amp;D and Process Development Lab Managers and Users </li>
<li>Anyone performing titrations in a 21 CFR Part 11 Compliant Environment </li>
<li>Those interested in data security and integrity</li>
</ul>
<blockquote>
<p><strong>Presenter:</strong> Kerri-Ann Blake (Titration Product Manager, Metrohm USA)</p>
</blockquote>
<blockquote>
<p><strong>Presenter:</strong> Daniel Schmidt (IT Systems Engineer, Metrohm USA)</p>
</blockquote>
<blockquote>
<p><strong>Presenter:</strong> Catherine Dold (Health &amp; Environment Writer, C&amp;EN Media Group)</p>
</blockquote>


C&EN: Peace-of-Mind with Secure Data Management

Peace-of-Mind with Secure Data Management

Kerri-Ann Blake, Product Manager for Titration, and Daniel Schmidt, IT Systems Engineer, will introduce you to the OMNIS data management platform.

<p><strong>The field of mass spectrometry has advanced tremendously over the last decade on both the hardware as well as the software side. Nevertheless, we still do not use the full capacity of the acquired proteomic data and several layers of information get lost in typical label-free quantification data-dependent acquisition proteomic datasets. A major source of this information loss is the presence of chimeric spectra, which contain fragment ions from multiple peptides, and the inability to fully interpret them.</strong></p>
<p>In this webinar, we explore how the CHIMERYS intelligent search algorithm gives us a handle to elucidate the hidden traces within chimeric spectra. We’ll also discuss how CHIMERYS increases instrument efficiency and protein identification rates through deconvolution of chimeric spectra, enabling new data acquisition strategies with wide-window acquisition.</p>
<h5>Attend this webinar to:</h5>
<ul>
<li>Learn how to interpret proteomics data from mass spectrometers more fully</li>
<li>Understand how CHIMERYS elucidates chimeric spectra</li>
<li>Deploy new wide-window acquisition methods to increase instrument efficiency for proteomics</li>
</ul>
<blockquote>
<p><strong>Presenter:</strong> Dr. Johanna Tüshaus (Postdoctoral Researcher, Technical University Munich)</p>
</blockquote>


Technology Networks: Hidden Traces – Using Chimeric Spectra To Boost Protein Identification Rates

Hidden Traces – Using Chimeric Spectra To Boost Protein Identification Rates

In this webinar, we explore how the CHIMERYS intelligent search algorithm gives us a handle to elucidate the hidden traces within chimeric spectra.

Using Chimeric Spectra To Boost Protein Identification Rates

<p><strong>Having comprehensive documentation of chemical analyses is crucial for any pharma QC laboratory. Capturing the entire analytical process – from sample preparation to crucial system components such as sensors and even the chemicals used for the analysis – requires the right data management solution. These solutions should also provide all the features needed for secure and efficient data management in networked processes within large, integrated organizations.</strong></p>
<p>In this upcoming webinar, we will discuss the latest advances in data management solutions for the pharmaceutical QC laboratory.</p>
<h5>Register now and join the discussion to learn:</h5>
<ul>
<li>How digitalization facilitates the integration of all relevant data – for seamless compliance and superior data integrity according to the ALCOA+ principles</li>
<li>How to create secure workflows and manage them efficiently within your organization</li>
<li>About the power of the One Click Audit Trail, integration of smart chemicals, and the next level of compliance and data integrity</li>
</ul>
<blockquote>
<p><strong>Presenter:</strong> Simon Böhm, PhD (Product Manager Software, Metrohm International Headquarters)</p>
</blockquote>
<blockquote>
<p>Simon Böhm is Product Manager of the OMNIS Software platform at the Competence Centre Software/Workflow/Platforms at Metrohm International Headquarters in Herisau, Switzerland. Simon started working for Metrohm in 2017, when he joined Metrohm Germany. In 2020 he moved on to join Metrohm International Headquarters. Simon studied chemistry at the University of Constance and earned his PhD from the ETH Zurich in physical chemistry.</p>
</blockquote>


Metrohm: Facing Pharma Lab Audits with Confidence

Facing Pharma Lab Audits with Confidence

In this upcoming webinar, we will discuss the latest advances in data management solutions for the pharmaceutical QC laboratory.

<p><strong>How do you know if your peak is “pure”? How do you know if your sample conforms to your gold standard? OpenLab CDS provides the tools to answer these questions. This seminar will show you how.</strong></p>
<blockquote>
<p><strong>Presenter:</strong> Richard Mutkoski (Laboratory Informatics Application Engineer, Agilent Technologies, Inc.)</p>
</blockquote>
<blockquote>
<p>Richard Mutkoski has been a field representative for over 27 years for both hardware and software. In his current role as an Informatics Application Engineer, Rich presents technical solutions of the OpenLab suite of products to customers worldwide. Prior to his tenure at Agilent Rich spent 15 years in a variety of Pharma QA/QC and R&amp;D positions providing him a unique understanding of those workflows, bottlenecks and challenges.</p>
</blockquote>


Agilent Technologies: Save Time Using the New Integration Optimizer in OpenLab CDS

Save Time Using the New Integration Optimizer in OpenLab CDS

How do you know if your peak is “pure”? How do you know if your sample conforms to your gold standard?

<h3><strong>Agenda</strong></h3>
<ul>
<li><p>9:55 Welcome and introduction</p>
</li>
<li><p><strong>10:00 SCIEX 7500 system hardware overview</strong></p>
</li>
<li><p><strong>10:30 SCIEX OS software overview — acquisition</strong></p>
</li>
<li><p><strong>11:00 Lab session part 1 – set up method and data acquisition (pre-recorded)</strong></p>
</li>
<li><p>11:45 Break</p>
</li>
<li><p><strong>12:15 Lab session part 2 - data review (pre-recorded)</strong></p>
</li>
<li><p><strong>13:00 SCIEX OS software overview - data processing</strong></p>
</li>
<li><p><strong>13:30 Question and answer</strong></p>
</li>
<li><p>14:00 Closing</p>
</li>
</ul>
<h3><strong>Your hosts for the day</strong></h3>
<h5>Dr Jianru Stahl-Zeng (Sr Technical Marketing Manager)</h5>
<h5>Jack Steed (Technical Specialist)</h5>


SCIEX: SCIEX 7500 System Virtual Tech Day

SCIEX 7500 System Virtual Tech Day

You will learn and experiment from our experts on how to acquire, review and process your data using SCIEX OS software and the SCIEX 7500 system.

LCMS webinars focusing on Software - page 38

Hidden Traces – Using Chimeric Spectra To Boost Protein Identification Rates

Facing Pharma Lab Audits with Confidence

Save Time Using the New Integration Optimizer in OpenLab CDS

SCIEX 7500 System Virtual Tech Day

Ask the Experts Panel: Pesticides by LC/MS

Peace-of-Mind with Secure Data Management

Empower Tips: Ask Me Anything About Creating Efficiency in Your Routine Workflow with Simple Automation Steps

What's That Peak? Structure Elucidation & Verification Virtual Symposium

What's That Peak? Structure Elucidation & Verification Virtual Symposium

Fall Back to Basics - Part 4: Informatics

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