Meeting EU Drinking Water Standards: The Critical Role of Sample Prep in Trace Contaminant Analysis

<h3><strong>Charged for Action: The Hidden Secrets of AEX Oligonucleotide Analysis</strong></h3><p><strong>Oligonucleotides—short, well-defined fragments of DNA or RNA—have become indispensable tools in modern life sciences. They underpin cutting-edge applications in molecular diagnostics, gene therapy, and pharmaceutical development. Before oligonucleotides can be confidently used in research or clinical settings, however, their quality must be rigorously assessed. Purity, sequence integrity, length distribution, and chemical modifications all play a decisive role in their performance.</strong></p><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/KNAUER_Oligos_Made_Easy_Part_2_AEX_2_45eee54fa0.jpg" alt="KNAUER: Oligos Made Easy - Part 2: AEX" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_KNAUER_Oligos_Made_Easy_Part_2_AEX_2_45eee54fa0.jpg 245w," sizes="100vw" width="1534" height="711"></p><p>Among the analytical techniques available today, <strong>anion exchange chromatography (AEX)</strong> stands out as a robust and highly informative method for characterizing oligonucleotides. By exploiting their intrinsic negative charge, AEX provides detailed insight into product composition and impurity profiles that are difficult to obtain by other approaches.</p><blockquote><p><a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/article/6808"><strong>Part 1 of the Oligos series: A Practical Guide to Oligonucleotide Production – From Concept to Final Product</strong></a></p></blockquote><h4><strong>How Scientists Read Oligonucleotides Using Charge</strong></h4><p>At the core of AEX lies a simple but powerful principle: oligonucleotides carry a negatively charged phosphate backbone. This charge increases in a predictable way with strand length and, to a lesser extent, sequence composition. Anion exchange chromatography uses this property to separate oligonucleotides based on differences in charge strength and interaction with a positively charged stationary phase.</p><p>As a result, AEX has become one of the most widely used analytical tools for evaluating oligonucleotide quality. It allows scientists to distinguish full-length products from truncated species, degradation products, or closely related variants—often with single-nucleotide resolution.</p><h4><strong>Why Ion Exchange Works: Binding, Retention, and Release</strong></h4><p>Ion exchange chromatography (IEX) relies on electrostatic interactions between analytes and a charged stationary phase. In the case of oligonucleotides, <strong>anion exchange</strong> is used, where negatively charged molecules interact with positively charged functional groups on the resin—most commonly quaternary amines.</p><p>The system consists of two main components:</p><ul><li><strong>Stationary phase:</strong> A positively charged resin that binds anionic analytes.</li><li><strong>Mobile phase:</strong> An aqueous buffer system, typically combined with a salt gradient, that controls both pH and elution strength.</li></ul><p>Retention in AEX is governed primarily by charge. Molecules with weaker overall charge interact less strongly with the stationary phase and elute earlier, while more highly charged oligonucleotides bind more tightly and require stronger elution conditions.</p><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/KNAUER_Oligos_Made_Easy_Part_2_AEX_Figure_1_IEX_overview_including_elution_order_dependency_on_analyte_charge_3419ad3075.jpg" alt="KNAUER: Oligos Made Easy - Part 2 AEX: Figure 1 IEX overview including elution order dependency on analyte charge." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_KNAUER_Oligos_Made_Easy_Part_2_AEX_Figure_1_IEX_overview_including_elution_order_dependency_on_analyte_charge_3419ad3075.jpg 245w," sizes="100vw" width="1583" height="654"></p><h4><strong>The Five-Step Workflow of Ion Exchange Chromatography</strong></h4><p>AEX separations generally follow a well-established sequence of steps:</p><ol><li><strong>Column equilibration</strong><br>The column is conditioned with the starting buffer until the baseline and pH are stable. This ensures that all charged sites on the resin are ready for interaction.</li><li><strong>Sample loading</strong><br>Samples, prepared in the starting buffer, are introduced under conditions that promote binding of the target oligonucleotides while minimizing retention of unwanted impurities.</li><li><strong>Washing</strong><br>The column is washed with the starting buffer to remove uncharged or weakly interacting components. The baseline stabilizes as these species are flushed away.</li><li><strong>Elution</strong><br>Bound oligonucleotides are released using a salt or pH gradient. Increasing ionic strength introduces competing ions that displace the analytes from the resin. Weakly bound species elute first; strongly charged oligonucleotides elute later.</li><li><strong>Regeneration and re-equilibration</strong><br>A high-salt buffer removes any remaining bound material. The column is then returned to starting conditions in preparation for the next analysis.</li></ol><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/KNAUER_Oligos_Made_Easy_Part_2_AEX_Figure_2_Five_steps_of_IEX_procedure_in_blue_outlined_using_an_example_chromatogram_in_red_38dd70b1b1.jpg" alt="KNAUER: Oligos Made Easy - Part 2 AEX: Figure 2  Five steps of IEX procedure (in blue), outlined using an example chromatogram (in red)." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_KNAUER_Oligos_Made_Easy_Part_2_AEX_Figure_2_Five_steps_of_IEX_procedure_in_blue_outlined_using_an_example_chromatogram_in_red_38dd70b1b1.jpg 245w," sizes="100vw" width="1583" height="674"></p><h4><strong>Why AEX Is Especially Effective for Oligonucleotides</strong></h4><p>The repeating phosphate backbone of oligonucleotides gives rise to a strong and uniform anionic character across a wide pH range (typically pH 7–9). This makes oligonucleotides exceptionally well suited for anion exchange separations.</p><p>Key factors influencing AEX behavior include:</p><ul><li><strong>Charge density:</strong> Longer oligonucleotides carry more negative charges and therefore exhibit stronger retention.</li><li><strong>Resin chemistry:</strong> Strong and weak anion exchangers differ in functional groups and pH stability, influencing selectivity and robustness.</li><li><strong>Buffer composition:</strong> The choice of buffering ions and salt gradients directly affects elution strength and resolution.</li></ul><p>Salt gradients—commonly based on chloride ions—compete with the oligonucleotide backbone for binding sites on the resin. By carefully tuning gradient slope and composition, analysts can achieve highly reproducible and finely resolved separations.</p><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/KNAUER_Oligos_Made_Easy_Part_2_AEX_Figure_3_Ionic_interactions_and_p_H_influence_on_Anion_Exchange_Chromatography_AEX_612e231ba4.jpg" alt="KNAUER: Oligos Made Easy - Part 2 AEX: Figure 3 Ionic interactions and pH influence on Anion Exchange Chromatography (AEX)." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_KNAUER_Oligos_Made_Easy_Part_2_AEX_Figure_3_Ionic_interactions_and_p_H_influence_on_Anion_Exchange_Chromatography_AEX_612e231ba4.jpg 245w," sizes="100vw" width="1547" height="593"></p><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/KNAUER_Oligos_Made_Easy_Part_2_AEX_Figure_4_AEX_buffer_selection_and_gradient_strategy_chromatogram_with_red_buffer_gradient_curve_53fd1bb11b.jpg" alt="KNAUER: Oligos Made Easy - Part 2 AEX: Figure 4 AEX buffer selection and gradient strategy (chromatogram with red buffer gradient curve)." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_KNAUER_Oligos_Made_Easy_Part_2_AEX_Figure_4_AEX_buffer_selection_and_gradient_strategy_chromatogram_with_red_buffer_gradient_curve_53fd1bb11b.jpg 245w," sizes="100vw" width="1600" height="327"></p><h4><strong>AEX as the Gold Standard for Oligonucleotide Separation</strong></h4><p>Anion exchange chromatography has become the method of choice for both analytical and preparative oligonucleotide workflows. Its advantages include:</p><ul><li><strong>High resolving power:</strong> Closely related impurities, including n–1 or n–2 species, can often be separated and quantified.</li><li><strong>Predictable retention behavior:</strong> In many cases, retention time correlates linearly with oligonucleotide length, simplifying identification.</li><li><strong>Compatibility with modified oligonucleotides:</strong> Even chemically modified sequences retain their anionic nature, allowing AEX to resolve both products and related impurities.</li><li><strong>Excellent control via gradient elution:</strong> Salt gradients provide precise tuning of selectivity and resolution.</li></ul><p>Typically, shorter or degraded oligonucleotides elute at lower ionic strength, while full-length products with higher charge density appear later in the chromatogram.</p><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/KNAUER_Oligos_Made_Easy_Part_2_AEX_Figure_5_AEX_Advantages_and_Challenges_7835521e34.jpg" alt="KNAUER: Oligos Made Easy - Part 2 AEX: Figure 5 AEX Advantages and Challenges." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_KNAUER_Oligos_Made_Easy_Part_2_AEX_Figure_5_AEX_Advantages_and_Challenges_7835521e34.jpg 245w," sizes="100vw" width="1535" height="707"></p><h4><strong>Final Perspective: Mastering AEX with Confidence</strong></h4><p>Anion exchange chromatography may appear complex at first, but with thoughtful optimization of pH, buffer composition, and gradient conditions, it becomes a highly precise and reliable tool for oligonucleotide analysis. When properly applied, AEX delivers sharp peaks, excellent resolution, and robust performance—even for challenging sequences and modifications.</p><p>For laboratories developing or refining oligonucleotide workflows, AEX offers both analytical depth and operational confidence.</p><blockquote><p>If you would like to discuss AEX method development or optimization for oligonucleotides, feel free to contact us at <strong>sales@knauer.net</strong>. More insights into practical oligonucleotide analysis will follow in our <i>“Oligos Made Easy”</i> series.</p><p>For technical questions or in-depth discussion, the author can be reached at <strong>kindler@knauer.net</strong></p></blockquote>

Oligos Made Easy - Part 2: AEX

<p><strong>Although modern agriculture needs to be based on accurate real-time data on soil, plant, and environmental conditions, affordable, sufficiently sensitive, and robust monitoring devices for this purpose are currently missing. According to scientists, low-cost sensors based on new smart materials and printing technologies offer a suitable solution. The results of multidisciplinary research thus far and proposals for the development of these devices have been presented by researchers from Czechia, Great Britain, Spain, and the USA in a review article in the prestigious journal </strong><a target="_blank" rel="noopener noreferrer" href="https://www.nature.com/articles/s41467-026-68778-6#Abs1"><i><strong>Nature Communications</strong></i></a><strong>.</strong></p><p>“<i>Sensor technologies for monitoring plant health are still in their infancy. Only simple sensors capable of monitoring basic parameters such as pH, soil moisture, or temperature are commonly available on the market. However, if farmers are to face current challenges, including climate change, and ensure sufficient food for humanity, they need much more effective tools that will enable them to monitor, for example, various pathogens, including bacteria and fungi. In our opinion, a suitable solution is a combination of new smart materials and printing for the production of inexpensive, durable, and scalable materials</i>,” said the article’s first author, <strong>David Panáček</strong> from CATRIN, who worked on the study during his internship at Imperial College London.</p><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/CATRIN_Soucasne_senzory_nestaci_Vedci_navrhuji_novou_cestu_ke_sledovani_zdravi_rostlin_b4014952a3.jpg" alt="CATRIN: David Panáček" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_CATRIN_Soucasne_senzory_nestaci_Vedci_navrhuji_novou_cestu_ke_sledovani_zdravi_rostlin_b4014952a3.jpg 208w," sizes="100vw" width="1536" height="1152"></p><p>Sensors for monitoring plant health, for example, lag behind similar devices used in human medicine. According to the authors, advances in materials science, which will enable the development of scalable sensor technologies, play a key role in this potential shift. “<i>Printing techniques, including inkjet printing, screen printing, aerosol printing, 3D printing, and direct laser writing, offer flexible ways to produce flexible sensors, large-area sensors, and sensors integrated directly into plants. The review article summarizes recent advances in printable low-dimensional materials for agricultural sensors, analyzes their physico-chemical properties in relation to sensor performance, and discusses key challenges and future opportunities requiring interdisciplinary integration</i>,” said one of the corresponding authors, <strong>Michal Otyepka</strong>.</p><p>The research brought together materials scientists, chemists, biologists, sensing experts, and printing technology specialists. Materials research and the use of graphene-based smart materials were led by scientists from CATRIN at Palacký University and VŠB-Technical University of Ostrava. Plant biologists from CATRIN contributed their knowledge of plant development, their responses to stress conditions, and the monitoring of their properties. Researchers from Imperial College London took care of the electronics, while representatives from<a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/companies/36"><strong> CEITEC – VUT</strong></a> focused on printing technologies. The team also included leading experts in sensing from the Catalan Institute of Nanoscience and Nanotechnology in Barcelona, and Joseph Wang of the University of California San Diego, the “father of sensing and electrochemistry,” also contributed his extensive knowledge.</p><p>“<i>This is a groundbreaking study on this topic, which has managed to attract some truly big names in their respective fields. Last but not least, the study demonstrates that interdisciplinarity at CATRIN gives rise to completely new and promising research topics, such as plant sensing and its use for studying plant-environment interactions and smart agriculture,</i>” added another corresponding author, <strong>Nuria De Diego</strong>.</p>

Current sensors are not good enough. Scientists propose a new way to monitor plant health

Article

Environment

Scientific article

Science and research

Video

The Czech Advanced Technology and Research Institute (CATRIN) at Palacký University is a cutting-edge scientific hub dedicated to advancing research in the fields of nanotechnology, biotechnology, and biomedicine. At its core, CATRIN boasts outstanding scientific teams, featuring international researchers. We emphasize interdisciplinarity, foster global collaborations, and work to translate our research findings into practical applications.

CATRIN

Organomation, founded in 1959, designs and manufactures high-quality nitrogen evaporators and extraction systems. Known for innovation and durability, their lab instruments are used globally for efficient sample preparation with strong customer support.

Organomation

Based in Berlin, KNAUER is a medium-sized, owner-managed company that has been serving the sciences since 1962. We develop and manufacture scientific instruments of superior quality for liquid chromatography, including: Analytical HPLC/UHPLC, Preparative HPLC, Fast protein liquid chromatography (FPLC), Multi-column chromatography/Simulated moving bed (SMB) chromatography. High pressure dosing, in-line detection systems, and osmometry are additional business areas.

KNAUER

Agilent Technologies

Bruker

Thermo Fisher Scientific

Wiley

SCIEX

Evosep Biosystems

Waters Corporation

<p><strong>Unlock new perspectives with our accurate mass 101 webinar series.</strong></p>
<p>Accelerate your workflows and expand your knowledge around accurate mass with these informative webinars.</p>
<p>In this webinar series, SCIEX applications scientists will explain the benefits of using accurate mass, sample analysis, instrument operation and data processing in real-time.</p>
<p>Get the inside scoop and register today!</p>
<h3><strong>Accurate mass 101: non-targeted analysis</strong></h3>
<p>Join us for this webinar to see how to discover the unknown using non-targeted analysis. We will showcase workflows and strategies to drive success with suspect screening and non-target identification. Discover the ability to identify peaks of interest and confidently identify unknown compounds.</p>


SCIEX: Accurate mass 101: non-targeted analysis

Accurate mass 101: non-targeted analysis

SCIEX applications scientists will explain the benefits of using accurate mass, sample analysis, instrument operation and data processing in real-time.

<p><strong>Unleash the power of DIA and enable the exploration of complex biological systems with unprecedented depth and precision for large-scale quantitative protein analysis combined with short methods on the Evosep One.</strong></p>
<h3><strong>Global proteomics with Evosep and DIA-PASEF</strong></h3>
<p>Recent progress in liquid chromatography and mass spectrometry, as well as sample preparation and search algorithms for spectra deconvolution and identification, plays an important role in bringing proteomics to the forefront of drug discovery and development. Nanoflow liquid chromatography coupled to tandem mass spectrometry is one of the most essential elements in a proteomics workflow. The four dimensional ion detection achieved with the latest generation of Bruker’s trapped ion mobility spectrometers led to superior speed and sensitivity. Evosep One liquid chromatography platform offers speed and robustness while maintaining, even exceeding previously achieved performance level with high-performing nano LC systems. We share here some results only possible with this combination of LC-MS and dia-PASEF.</p>
<blockquote>
<p><strong>Presenter:</strong> Anh Reynolds (Scientist, Bristol Myers Squibb)</p>
</blockquote>
<h3><strong>Extending sensitivity limits with Evosep and Slice-PASEF</strong></h3>
<p>Sensitivity improvements in LC-MS proteomics have recently led to exciting new applications, such as comprehensive proteomic profiling of single cells or 2-dimensional proteomics of tissue slices. In this talk, I will describe Slice-PASEF, a new acquisition technology for timsTOF instruments, which, in combination with the DIA-NN data analysis software, provides a fold-change signal boost. When paired with the Evosep system, Slice-PASEF allows to achieve high sensitivity in robust high-throughput workflows, paving a way for accessible sensitive proteomics on a large scale.</p>
<blockquote>
<p><strong>Presenter:</strong> Vladim Demichev (Group Leader, Quantitative ProteomicS, Charité – Universitätsmedizin Berlin)</p>
</blockquote>


Pixabay/sethink: Revolutionizing proteomics with Evosep-DIA

Revolutionizing proteomics with Evosep-DIA

Global proteomics with Evosep and DIA-PASEF & Extending sensitivity limits with Evosep and Slice-PASEF.

<p><strong>LC-MS/MS is rapidly becoming the gold standard for quantifying different classes of analytes in endocrinology research.  The power of greater selectivity and analytical sensitivity using LC-MS/MS is critical in understanding the status of endocrine biochemical pathways.  The enhanced performance observed on these platforms provides increased confidence in the results, enabling you to make better decisions based on more accurate information.</strong></p>
<p>Greater analytical sensitivity is a goal that drives improvements in precision, leading to clearer insights into results down to low physiological concentrations.  Here we will discuss the latest introduction to the Waters MassTrak™ IVD system family, which is shown to be analytically sensitive and efficient, helping to reduce cost and improve the quality of your data. </p>
<p>Learning objectives: </p>
<ul>
<li><p><strong>An introduction to the latest addition to Waters MassTrak IVD System for clinical laboratories</strong></p>
</li>
<li><p><strong>The importance of selectivity in endocrinology research in obtaining high quality data</strong></p>
</li>
<li><p><strong>How the latest addition to Waters MassTrak IVD System addresses the needs of those key endocrinology research applications, including steroid hormone analysis</strong></p>
</li>
</ul>
<blockquote>
<p><strong>Presenter:</strong> Dominic Foley (Principal Scientist, Waters Corporation)</p>
</blockquote>
<blockquote>
<p>Dominic is a Principal Scientist working at Waters Mass Spectrometry Headquarters in Wilmslow, UK. Having joined the company in September 2013, he has specialized in the development of LC-MS/MS clinical research applications, with expertise in steroid hormone analysis and protein/peptide quantification. In his role, he also collaborates with clinical customers in the development and implementation of LC-MS/MS methods in their laboratories.</p>
</blockquote>
<blockquote>
<p>Dominic has worked in separation sciences for 14 years, following his ambition of improving the quality of healthcare. He started his career with ICON Development Solutions, a CRO in the north of the UK, where he worked as a LC-MS/MS method development and validation scientist supporting DMPK studies in drug discovery. In 2012, he joined Thermo Fisher Scientific to work in their Chromatography R&amp;D team as a Technical Support Specialist, working on the development of new chromatography and sample preparation products.</p>
</blockquote>
<blockquote>
<p>Dominic received his BSc (Hons) in Biochemistry from the University of Manchester in 2006. He has presented his work at conferences around the world and has several publications in both the Microchemical Journal and the Journal of Chromatography A.</p>
</blockquote>
<blockquote>
<p><strong>Presenter:</strong> David Ballantyne (Principal Product Manager, Waters Corporation)</p>
</blockquote>
<blockquote>
<p>David Ballantyne is a Principal Product Manager at Waters Corporation, working within the Global Clinical Diagnostics Portfolio Management team. Having joined the company in 2005, he has held multiple roles throughout Manufacturing and R&amp;D. David has a background in Electrical and Mechanical Engineering and began his career at Waters supporting GC-MS/MS technologies including Tandem Quadrupole and Magnetic Sector Mass Spectrometry.</p>
</blockquote>
<blockquote>
<p>In 2018 he moved to his role in Product Management where is focus changed to Clinical Diagnostics, with a particular focus on LC – MS/MS Instrumentation and Informatics solutions. Over the last 5 years David has spent much of his time focused on understanding customer problems and translating them into new solutions.</p>
</blockquote>


Waters Corporation: The Evolving Power of LC-MS/MS Technology within Endocrinology

The Evolving Power of LC-MS/MS Technology within Endocrinology

Here we will discuss the latest introduction to the Waters MassTrak™ IVD system family for clinical laboratories.

The Evolving Power of LC-MS/MS within Endocrinology

<p>Dear user and other PaSER interested customers,</p>
<p>You are hereby invited to our 2nd Virtual PaSER Workshop. A lot of new possibilities has been introduced since last time – including, but not limited to PaSER Novor for de novo sequencing of dda-PASEF® data on the PaSER Box as well as TIMS DIA-NN 2.0 -which will be demonstrated during this Workshop. Data virtualization has been a requested feature – and this is now possible with Mass Dynamics for PaSER. Not limited to above – stay tuned for even more approaching features, we will discuss as well.</p>
<h3><strong>The tentative agenda looks like this:</strong></h3>
<h5><strong>1:00 – 1:15 pm:  Introduction</strong></h5>
<ul>
<li>Willy Bjørklund</li>
</ul>
<h5><strong>1:15 – 1: 35pm:  New Applications on the timsTOF Platform</strong></h5>
<ul>
<li>Lars Kristensen (Field Application Specialist)</li>
</ul>
<h5><strong>1:35 – 1:55 pm:  PaSER as a bioinformatic platform - what’s new and what’s coming</strong></h5>
<ul>
<li>Jonathan Krieger (Software R&amp;D Department)</li>
</ul>
<h5><strong>5 minute break</strong></h5>
<h5><strong>2:00 – 2:20 pm: Live Demo/workshop of PaSER</strong></h5>
<ul>
<li>Tharan Srikumar (Software R&amp;D Department)</li>
</ul>
<h5><strong>2:20 – 2:50 pm: Mass Dynamics</strong></h5>
<ul>
<li>Mark Condina and Giuseppe Infusini</li>
</ul>
<h5><strong>2:50 – 3:10 pm: Questions and discussions</strong></h5>
<h5><strong>3:10 – 3:15 pm:  Conclusion</strong></h5>
<ul>
<li>Willy Bjørklund</li>
</ul>
<p>We aim for a lively interaction so take the timing as an indication. Please feel free to share with colleagues or others you think could benefit from attending this workshop. Looking forward to “meet”</p>


Bruker Virtual PaSER Workshop

Dear user and other PaSER interested customers, You are hereby invited to our 2nd Virtual PaSER Workshop. 

<p><strong>This webinar will focus on untargeted LC/MS Omics solutions that will provide the biological insights needed to produce meaningful results. An emphasis will be spent on an End-to-End HILIC metabolomics workflow for our LC/Q-TOF platform.</strong></p>
<p>This includes automate sample preparation for cells and plasma, a Bio LC that is fit for purpose for metal sensitive analysis, robust and transferable HILIC chromatography, the 6546 LC/Q-TOF data independent analysis of samples, with an ID workflow that includes a new HILIC PCDL with 500 core metabolism analytes with spectra and retention time annotation.</p>
<p>For unknowns not found in Agilent PCDLs, SIRIUS/CSI:FingerID which uses AI predict structure from spectra and conducts online identification search will be demonstrated. A complementary targeted multi-omics workflow will be briefly mentioned before sharing the new Ion Mobility and ExD technologies for high end LC/MS Omics investigations.</p>
<blockquote>
<p><strong>Presenter:</strong> Karen E. Yannell (Omics Workflow Specialist, Agilent Technologies)</p>
</blockquote>
<blockquote>
<p>Karen E. Yannell is an Omics Workflow Specialist at Agilent Technologies. She is an expert in small molecule high throughput analysis by triple quadrupole (TQ) and quadrupole time-of-flight (Q-TOF) mass spectrometry for applied markets. Her recent work has focused on developing targeted metabolomics methods and routine Q-TOF workflows for both expert and non-expert users alike. Previously, Karen worked on forensic, metabolomics, and biomarker discovery applications with ambient ionization sources. She received her PhD in Analytical Chemistry from Purdue University where studied under the advisement of Prof. R. Graham Cooks. </p>
</blockquote>
<blockquote>
<p><strong>Presenter:</strong> Dr. Martin Graf-Utzmann (Editor, Wiley Analytical Science, Moderator)</p>
</blockquote>
<blockquote>
<p>Dr. Martin Graf-Utzmann has a Ph.D. in Theoretical Chemistry and is an editor for Wiley Analytical Science. His interests are mainly Laboratory Automation, Digitalization, and Lab Equipment.</p>
</blockquote>


Wiley Analytical Science: Jump Start Your LC/Q-TOF Omics Investigation with Reliable Workflows

Jump Start Your LC/Q-TOF Omics Investigation with Reliable Workflows

This webinar will focus on untargeted LC/MS Omics solutions that will provide the biological insights needed to produce meaningful results.

Your LC/Q-TOF Omics Investigation with Reliable Workflows

<p><strong>This year we invite you to ignite your mind to Be Curious about what Ion chromatography (IC) can do with its versatility in many detections across the IC application spectrum to improve your workflows and solve challenges.</strong></p>
<p>During this three day virtual event, learn from a panel of experts how the latest IC applications are helping colleagues achieve breakthroughs, and walk away with valuable information on how advancements in IC can increase laboratory analytical capabilities. </p>
<p>Although each day is convenient for a specific region, you can attend any session on any day. All sessions and workshops will be available for on-demand viewing after the event concludes.</p>
<h3><strong>Day 1 Agenda | April 18 | North and South America</strong></h3>
<p>Discover IC column development, and where it’s headed with mass spectrometry, as they can be an integral detection for carbohydrate profiling and environmental metabolites characterization. Learn how standalone IC can solve industrial samples and degradation products with comprehensive IC information, all at once.</p>
<h5><strong>11:30 EDT: Opening Plenary: The Past, Present, and Future of IC Column Development</strong></h5>
<ul>
<li>Alexandra Zatirakha (Thermo Fisher Scientific, IC/SP R&amp;D, Sunnyvale)</li>
</ul>
<h5><strong>12:30 EDT: Characterizing Carbohydrate Degradation Products from Bacteroides Thetaiotaomicron to Understand Bacterial Dextran Utilization in the Gut</strong></h5>
<ul>
<li>Neil Rumachik (Thermo Fisher Scientific, IC/SP R&amp;D, Sunnyvale)</li>
<li>Fiona Cuskin (Newcastle University, UK)</li>
</ul>
<h5><strong>1:30 PM EDT: Toward Comprehensive Chemical Characterization of Metabolomic Using Orbitrap Fusion Tribrid Mass Spectrometer Coupled with Ion and Liquid Chromatography Techniques: Dissolved Organic Matter as an Example</strong></h5>
<ul>
<li>Hussain Abdulla (Department of Physical and Environmental Sciences, Texas A&amp;M University-Corpus Christi)</li>
</ul>
<h5><strong>2:30 PM EDT: The Use of Ion Chromatography in the Monitoring of Degradation Products from the Electrochemical Treatment Of Organic Compounds</strong></h5>
<ul>
<li>Emily Tossi (Federal University of Rio Grande do Norte)</li>
</ul>
<h5><strong>3:30 PM EDT: Ion Chromatography as a Tool for Industrial Problem Solving</strong></h5>
<ul>
<li>Isaac Negrete and Stephen Fosdick (Dow)</li>
</ul>
<h5><strong>4:30 PM EDT: Workshop: Everything, Everywhere, All at Once IC!</strong></h5>
<ul>
<li>Kelly Femrite (IC/SP Product Support, Sunnyvale)</li>
<li>Carl Fisher (IC/SP Product Marketing, Sunnyvale)</li>
<li>Criss Corchard-Keeler (Service Marketing Manager, CMD/MOL Thermo Fisher Scientific)</li>
</ul>


Thermo Fisher Scientific: IC Symposium 2023

IC Symposium 2023 - Day 1

Join us for the 2023 Global IC Symposium! North and South America

<p><strong>This year we invite you to ignite your mind to Be Curious about what Ion chromatography (IC) can do with its versatility in many detections across the IC application spectrum to improve your workflows and solve challenges.</strong></p>
<p>During this three day virtual event, learn from a panel of experts how the latest IC applications are helping colleagues achieve breakthroughs, and walk away with valuable information on how advancements in IC can increase laboratory analytical capabilities. </p>
<p>Although each day is convenient for a specific region, you can attend any session on any day. All sessions and workshops will be available for on-demand viewing after the event concludes.</p>
<h3><strong>Day 3 Agenda | April 20 | Asia Pacific</strong></h3>
<p>Expand the world of electrochemistry applications and IC hyphenation with high-resolution mass spectrometry and understand how Inductively Coupled Plasma Spectrometry can speciate isotopes while simplifying Pharmacopeia QC analysis and keeping your IC in top-notch condition.</p>
<h5><strong>11:00 AM SGT: Opening Plenary: Electrochemical Detection and Its Application in Ion Chromatography</strong></h5>
<ul>
<li>Yan Liu (Thermo Fisher Scientific, IC/SP R&amp;D, Sunnyvale)</li>
</ul>
<h5><strong>12:00 PM SGT: Application of Anion Chromatography Coupled High-Resolution Mass Spectrometry to Increase Cellular Metabolite Coverage</strong></h5>
<ul>
<li>Vinzenz Hofferek (McConville Lab, Department of Biochemistry and Pharmacology)</li>
</ul>
<h5><strong>1:00 PM SGT: Analysis of Anhydrosugars and Xylooligosaccharides by Ion Chromatography with Electrospray Lithium Cationisation-Triple Quadrupole Tandem Mass Spectrometry</strong></h5>
<ul>
<li>Dr. Estrella Sanz Rodriguez (Natural Products Analysis, Australian Centre for Research on Separation Science (ACROSS), University of Tasmania)</li>
</ul>
<h5><strong>2:00 PM SGT: You Gotta Keep ‘Em Separated: Novel IC Applications for Subsequent Isotopic Analyses by Rapid and/or Hyphenated Techniques</strong></h5>
<ul>
<li>Dr. Brandon Mahan (College of Science and Engineering, James Cook University)</li>
<li>Grace Manestar (IsoTropics PhD Candidate)</li>
</ul>
<h5><strong>3:00 PM SGT: Use of Ion Chromatography in Pharmaceutical Quality Control</strong></h5>
<ul>
<li>Catherine Frankis and Stella Planou (Reading Scientific Services Ltd., Reading Science Centre, Whiteknights Campus)</li>
</ul>
<h5><strong>4:00 PM SGT: Tips on Keeping Your Ion Chromatography in Top-Notch Condition</strong></h5>
<ul>
<li>Ryan Ong (Thermo Fisher Scientific, IC/SP Application Sales Support, Singapore)</li>
</ul>


IC Symposium 2023 - Day 3

Join us for the 2023 Global IC Symposium! Day 3 | Asia Pacific.

<ul>
<li><p>Wait what?! Who knew!? There are so many things that a mass spec can do.</p>
</li>
<li><p>I&#39;ve used it for years, yes this may be true.</p>
</li>
<li><p>I&#39;ve processed some samples, more than a few.</p>
</li>
<li><p>But between me and you, I had no idea all the things it could do!</p>
</li>
<li><p>From sample prep to separation, you just won&#39;t believe…</p>
</li>
<li><p>All of the tips and tricks we have up our sleeve.</p>
</li>
<li><p>So, take a few minutes, about 15 should do.</p>
</li>
<li><p>And learn all the things your mass spec can do.</p>
</li>
</ul>
<p><strong>An easy to follow guide detailing how to set MS scan parameters for an LCMS experiment will be presented.  Scan speeds for reproducible quantitation, chromatographic resolution, retention time definition, proper threshold adjustment for optimum datafile size, and how this can result in faster processing times will all be discussed.</strong></p>
<blockquote>
<p><strong>Presenter:</strong> Rebecca Glaskin, Ph.D. (LC/MS Application Scientist, Agilent Technologies, Inc.)</p>
</blockquote>
<blockquote>
<p>Rebecca Glaskin is an LC/MS Application Scientist at Agilent with a focus on BioPharma applications supporting the LC-Q/TOF and IM-QTOF platforms. Prior to joining Agilent, Rebecca received her Ph.D. in analytical chemistry from Indiana University in the lab of Professor David Clemmer. While there she designed and constructed home-built instruments, pushing the limits of the mobility resolution that can be obtained with a circular drift tube for the separation of biomolecules (peptides, proteins, carbohydrates, and metabolites). While there, she also studied hydrogen/deuterium exchange of proteins in the gas-phase as a function of time and pressure. Rebecca then went to Boston University as a Postdoctoral Associate in the lab of Professor Catherine Costello to develop a database containing collision cross section values for glycans, peptides, and glycopeptides utilizing Agilent Technologies 6560 IM-QTOF. This database can be used to determine how the collision cross section is altered with the addition of individual saccharide units. The trendlines obtained from this database will be used to predict collision cross sections for glycopeptides based on the conformation and structure of the specific glycoform.</p>
</blockquote>
<blockquote>
<p><strong>Presenter:</strong> Jim Lau, PhD (HRAMS LC/MS Application Engineer, Agilent Technologies, Inc.)</p>
</blockquote>
<blockquote>
<p>Jim Lau received his Ph.D. From the University of Colorado. There he worked in the laboratories of Peter Albersheim and Robert Shapiro. This work involved the structure elucidation of plant natural products and, in particular, elucidating the structures of oligosaccharides derived from plant cell wall polysaccharides. This work was done in the early days of LCMS and made use of DLI-LCMS and (at the time) recently invented Thermospray-LCMS.</p>
</blockquote>
<blockquote>
<p>After the completion of his Ph.D., Jim joined Hewlett-Packard to work on LCMS ionization techniques with Paul Goodley during the early days of Electrospray-LCMS. He then moved to work on field applications and collaborations with Hewlett-Packard’s LCMS customers. More than 30 years later, Jim continues to work with Agilent’s customers in High Resolution Accurate Mass applications of LCMS. While involved in many areas of HRAMS LCMS, his recent work has focused on the area of Screening/Qual&amp;Quant of small molecules and on the high throughput confirmation of oligonucleotide synthesis.</p>
</blockquote>


Agilent Technologies: How to set MS Scan Parameters for your LCMS Experiment

How to set MS Scan Parameters for your LCMS Experiment

An easy to follow guide detailing how to set MS scan parameters for an LCMS experiment will be presented.

<p>Targeted workflows are particularly interesting for identifying established biomarkers in various applications. The focus on these workflows, coupled with technological advancements, is continually expanding the boundaries of high-throughput targeted analysis.</p>
<h3><strong>Next Generation of absolute protein Quantitation in Biological Matrixes; higher multiplexicity while faster, robust and sensitive</strong></h3>
<ul>
<li><strong>Presenter:</strong> Dr. Christoph Borchers (Mcgill university)</li>
</ul>
<p>The accurate and rapid determination of protein concentrations using multiplexed multiple reaction monitoring (MRM) with isotope-labeled internal standards has the potential to revolutionize biochemistry and medical sciences. The combination of an Evosep One LC with an Agilent QQQ6495C allows a several-fold reduction of analysis time while maintaining high multiplexicity. As an example, we show the use of a multi-protein method for the absolute quantitation of 270 proteins, combined with metabolomics and Artificial Intelligence (AI), to analyze the plasma of a COVID cohort for prediction of survival. Moreover, optimization of the Evosep/6495C combination allowed adaptation of the 270-protein MRM method to a 24-minute gradient, thus doubling the throughput compared to before.</p>
<h3><strong>Development of a LC-MS/MS Method for Measurement of Fusion Sarcoma Proteins in Plasma</strong></h3>
<ul>
<li><strong>Presenter:</strong> Phd. AnThony Maus (Clinical Mass Spec. Development Laboratory, Mayo Clinic)</li>
</ul>
<p>Sarcoma is general term for a group of over 70 cancers that are classified using genomic techniques to identify characteristic gene fusions in tumor tissues. However, this technique requires relatively invasive biopsies and is not conducive to monitoring disease progression or response to treatment. Measurements of fusion protein biomarkers in plasma would overcome these limitations. We sought to develop an LC-MS/MS method capable of performing these measurements by conducting discovery experiments on cell lines with an EvoSep coupled to an Exploris 480 mass spectrometer. Using these data, we developed a targeted method and performed measurements in plasma samples.</p>


Targeted Workflows

Targeted workflows are particularly interesting for identifying established biomarkers in various applications.

Targeted LC/MS Workflows

<p>Webinar in collaboration with the Polish Society of Metabolomics.</p>
<h3><strong>14:00 - 14:20 Benefits of Trapped Ion Mobility Spectrometry (TIMS) for Metabolomics and Lipidomics</strong></h3>
<ul>
<li><strong>Presenter:</strong> Dr. Stefanie Wernisch ( Business Development Manager Metabolomics/Multiomics, Bruker Daltonics Bremen, Germany)</li>
</ul>
<p>After revolutionizing the area of proteomics, trapped ion mobility spectrometry (TIMS) is now conquering the fields of metabolomics and lipidomics. Bruker&#39;s unique timsTOF technology addresses the complexity of biological samples by adding an orthogonal dimension of separation before mass spectrometric analysis, thus reducing the complexity of MS/MS data and improving annotation confidence for untargeted analysis. The ability to determine Collisional Cross Sections (CCS) can further aid identification and outlier detection in lipidomics studies.</p>
<h3><strong>14:20 - 14:40 SpatialOMx® for in-depth 4D-Lipidomics™</strong></h3>
<ul>
<li><strong>Presenter:</strong> Dr. Janina Oetjen (Application Specialist MALDI Imaging, Bruker Daltonics Bremen, Germany)</li>
</ul>
<p>The combined possibilities of ESI and MALDI in one instrument enables powerful SpatialOMx® applications. In short, ion mobility-based annotation can connect confident LC-TIMS-MS/MS identification with meaningful spatial information from MALDI Imaging, specifically in the field of lipidomics. Further, segmentation results based on lipid imaging can define specific regions of interest for subsequent, in-depth analysis. Thus, the information content of MALDI Imaging can be enhanced with SpatialOMx® workflows on the timsTOF fleX platform.</p>
<h3><strong>14:40 - 15:00 Application Round-up: 4D-Metabolomics™ and 4D-Lipidomics™</strong></h3>
<ul>
<li><strong>Presenter:</strong> Dr. Matthias Szesny (Application Scientist Metabolomics, Bruker Daltonics Bremen, Germany)</li>
</ul>
<p>We will highlight the capabilities of the timsTOF platform and introduce new applications, method developments, and software solutions in the field of metabolomics and lipidomics. Those help scientists gain a faster and deeper understanding of diverse research areas such as health and disease as well as agriculture.</p>


Bruker: Expanding the Horizons of 4D-Metabolomics and 4D-Lipidomics

Expanding the Horizons of 4D-Metabolomics and 4D-Lipidomics

Benefits of TIMS for Metabolomics and Lipidomics. SpatialOMx for in-depth 4D-Lipidomics, Application Round-up: 4D-Metabolomics and 4D-Lipidomics.

LCMS webinars focusing on LC/MS/MS - page 61