<div>Mulching films used in agriculture gradually break down and can become a significant source of <a target="_blank" rel="noopener noreferrer" href="https://gcms.labrulez.com/library?search=microplastics">microplastics</a> in soils. A new study shows that these particles affect not only soil structure but also microbial life and nitrogen cycling.“We wanted to understand how microplastics from both conventional and biodegradable mulching films influence soil properties, microbial diversity and functions in real conditions,” explains Klára Šmídová, the study’s co-author . “That’s why we added realistic amounts of microplastics to soils in experimental plots in Finland, Germany, and Spain, monitored them over two growing seasons, and compared them with the control plots.”The results revealed that microplastics alter soil characteristics and microbial communities, although not always in the same way. “ However, a consistent finding was the decline in microbial activity associated with key processes, including nitrogen cycling, with negative effects increasing at higher microplastic concentrations and becoming more pronounced in the second year,” Šmídová adds. Because the microplastic levels used in the study reflect amounts already found in European soils, the findings highlight real risks to soil health. “We also found that the effects were stronger in southern than in northern Europe, likely due to differences in climate and soil conditions. This means that protecting soils from plastic pollution will require region-specific risk assessments,” she says.Over 60% of European soils are estimated to be unhealthy, with ongoing degradation driven by unsustainable land use, contamination, climate change, and extreme weather events. In response, the EU Parliament adopted the Soil Monitoring Law in October 2025, aiming to achieve healthy soils across Europe by 2050. “The law highlights the role of soil health in food security, water quality, and climate objectives, and establishes consistent soil monitoring across Member States. It includes measures to support farmers and foresters, while also creating a public list of potentially contaminated sites and an indicative watch list of emerging soil contaminants,” explains Klára Šmídová.The project brought together 19 research groups across Europe. “After each season, hundreds of samples were colle cted and sent to partners for analysis. This paper focuses on soil properties, microbial diversity, and greenhouse gas emissions. Two more manuscripts—on plastic additives and microplastic fate, and on soil invertebrates—are in preparation. Together, they will give us a complete picture of how agricultural microplastics affect soil ecosystems,” concludes Klára Šmídová.<img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/RECETOX_Vysledky_nove_studie_ukazaly_ze_mikroplasty_meni_vlastnosti_pudy_a_mikrobialni_spolecenstva_i_v_realnych_podminkach_6ee3413a7b.jpg" alt="RECETOX: New Study Reveals How Agricultural Plastics Impact Soil Health In Real Conditions" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_RECETOX_Vysledky_nove_studie_ukazaly_ze_mikroplasty_meni_vlastnosti_pudy_a_mikrobialni_spolecenstva_i_v_realnych_podminkach_6ee3413a7b.jpg 171w," sizes="100vw" width="738" height="674"><blockquote><h4>The Original article</h4><h4><a target="_blank" rel="noopener noreferrer" href="https://www.sciencedirect.com/science/article/pii/S0269749125015866?via%3Dihub#sec2">Conventional and biodegradable agricultural microplastics: effects on soil properties and microbial functions across a European pedoclimatic gradient</a></h4>Šmídová K., Soinne H., Kim S. W., Tirronniemi J., Meffe R., Redondo-Hasselerharm P. E., Braun M., Rillig M. C., Fritze H., Adamczyk B., Nikama J., Kaseva J., Saartama V., Amelung W., Hurley R., Hofman J., Nizzetto L., Selonen S., Velmala S.Environ. Pollut. 386 (2025) 127212<a target="_blank" rel="noopener noreferrer" href="https://doi.org/10.1016/j.envpol.2025.127212">10.1016/j.envpol.2025.127212</a><h6>licensed under <a target="_blank" rel="noopener noreferrer" href="https://creativecommons.org/licenses/by/4.0/">CC-BY 4.0</a></h6></blockquote><h4>Abstract</h4><div>Agricultural plastics like mulching films may become a major source of microplastic (MP) soil contamination during their degradation and fragmentation. This study investigates the effects of agricultural MPs from conventional (linear low-density polyethylene, PE) and biodegradable (starch-blended polybutylene adipate co-terephthalate, PBAT-BD) mulching films on soil physicochemical properties, aggregation, microbial diversity and functions, litter decomposition, and greenhouse gases emissions (GHG). For this purpose, MPs were mixed into soils at realistic MP concentrations of 0.005&nbsp;% and 0.05&nbsp;% (w/w) in 2022 on experimental plots in three EU countries representing different pedoclimatic conditions (Finland, Germany and Spain), followed by monitoring of the above-mentioned variables in the subsequent growing seasons 2022 and 2023. We found several significant MP-induced effects for soil properties, aggregation, microbial diversity, litter decomposition, and GHG, but the effect endpoints were less pronounced or varied considerably. Contrarily, microbial activity, contributing to soil functions such as nitrogen cycling, was consistently reduced by both conventional and biodegradable MPs. The reductions were more pronounced after the second season and for the higher MP treatment. As the higher MP concentration (i.e., 0.05&nbsp;% w/w) is environmentally relevant in Europe, our findings emphasize the potential effects of environmentally relevant MP concentrations on soil health. Furthermore, the effects increased from north to south, probably modulated by varying pedoclimatic conditions, inducing reflection of a need for regionally tailored risk</div></div>

New Study Reveals How Agricultural Plastics Impact Soil Health In Real Conditions

<h3>Charged for Action: The Hidden Secrets of AEX Oligonucleotide Analysis</h3>Oligonucleotides—short, well-defined fragments of DNA or RNA—have become indispensable tools in modern life sciences. They underpin cutting-edge applications in molecular diagnostics, gene therapy, and pharmaceutical development. Before oligonucleotides can be confidently used in research or clinical settings, however, their quality must be rigorously assessed. Purity, sequence integrity, length distribution, and chemical modifications all play a decisive role in their performance.<img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/KNAUER_Oligos_Made_Easy_Part_2_AEX_2_45eee54fa0.jpg" alt="KNAUER: Oligos Made Easy - Part 2: AEX" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_KNAUER_Oligos_Made_Easy_Part_2_AEX_2_45eee54fa0.jpg 245w," sizes="100vw" width="1534" height="711">Among the analytical techniques available today, anion exchange chromatography (AEX) stands out as a robust and highly informative method for characterizing oligonucleotides. By exploiting their intrinsic negative charge, AEX provides detailed insight into product composition and impurity profiles that are difficult to obtain by other approaches.<blockquote><a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/article/6808">Part 1 of the Oligos series: A Practical Guide to Oligonucleotide Production – From Concept to Final Product</a></blockquote><h4>How Scientists Read Oligonucleotides Using Charge</h4>At the core of AEX lies a simple but powerful principle: oligonucleotides carry a negatively charged phosphate backbone. This charge increases in a predictable way with strand length and, to a lesser extent, sequence composition. Anion exchange chromatography uses this property to separate oligonucleotides based on differences in charge strength and interaction with a positively charged stationary phase.As a result, AEX has become one of the most widely used analytical tools for evaluating oligonucleotide quality. It allows scientists to distinguish full-length products from truncated species, degradation products, or closely related variants—often with single-nucleotide resolution.<h4>Why Ion Exchange Works: Binding, Retention, and Release</h4>Ion exchange chromatography (IEX) relies on electrostatic interactions between analytes and a charged stationary phase. In the case of oligonucleotides, anion exchange is used, where negatively charged molecules interact with positively charged functional groups on the resin—most commonly quaternary amines.The system consists of two main components:<ul><li>Stationary phase: A positively charged resin that binds anionic analytes.</li><li>Mobile phase: An aqueous buffer system, typically combined with a salt gradient, that controls both pH and elution strength.</li></ul>Retention in AEX is governed primarily by charge. Molecules with weaker overall charge interact less strongly with the stationary phase and elute earlier, while more highly charged oligonucleotides bind more tightly and require stronger elution conditions.<img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/KNAUER_Oligos_Made_Easy_Part_2_AEX_Figure_1_IEX_overview_including_elution_order_dependency_on_analyte_charge_3419ad3075.jpg" alt="KNAUER: Oligos Made Easy - Part 2 AEX: Figure 1 IEX overview including elution order dependency on analyte charge." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_KNAUER_Oligos_Made_Easy_Part_2_AEX_Figure_1_IEX_overview_including_elution_order_dependency_on_analyte_charge_3419ad3075.jpg 245w," sizes="100vw" width="1583" height="654"><h4>The Five-Step Workflow of Ion Exchange Chromatography</h4>AEX separations generally follow a well-established sequence of steps:<ol><li>Column equilibration The column is conditioned with the starting buffer until the baseline and pH are stable. This ensures that all charged sites on the resin are ready for interaction.</li><li>Sample loading Samples, prepared in the starting buffer, are introduced under conditions that promote binding of the target oligonucleotides while minimizing retention of unwanted impurities.</li><li>Washing The column is washed with the starting buffer to remove uncharged or weakly interacting components. The baseline stabilizes as these species are flushed away.</li><li>Elution Bound oligonucleotides are released using a salt or pH gradient. Increasing ionic strength introduces competing ions that displace the analytes from the resin. Weakly bound species elute first; strongly charged oligonucleotides elute later.</li><li>Regeneration and re-equilibration A high-salt buffer removes any remaining bound material. The column is then returned to starting conditions in preparation for the next analysis.</li></ol><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/KNAUER_Oligos_Made_Easy_Part_2_AEX_Figure_2_Five_steps_of_IEX_procedure_in_blue_outlined_using_an_example_chromatogram_in_red_38dd70b1b1.jpg" alt="KNAUER: Oligos Made Easy - Part 2 AEX: Figure 2 Five steps of IEX procedure (in blue), outlined using an example chromatogram (in red)." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_KNAUER_Oligos_Made_Easy_Part_2_AEX_Figure_2_Five_steps_of_IEX_procedure_in_blue_outlined_using_an_example_chromatogram_in_red_38dd70b1b1.jpg 245w," sizes="100vw" width="1583" height="674"><h4>Why AEX Is Especially Effective for Oligonucleotides</h4>The repeating phosphate backbone of oligonucleotides gives rise to a strong and uniform anionic character across a wide pH range (typically pH 7–9). This makes oligonucleotides exceptionally well suited for anion exchange separations.Key factors influencing AEX behavior include:<ul><li>Charge density: Longer oligonucleotides carry more negative charges and therefore exhibit stronger retention.</li><li>Resin chemistry: Strong and weak anion exchangers differ in functional groups and pH stability, influencing selectivity and robustness.</li><li>Buffer composition: The choice of buffering ions and salt gradients directly affects elution strength and resolution.</li></ul>Salt gradients—commonly based on chloride ions—compete with the oligonucleotide backbone for binding sites on the resin. By carefully tuning gradient slope and composition, analysts can achieve highly reproducible and finely resolved separations.<img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/KNAUER_Oligos_Made_Easy_Part_2_AEX_Figure_3_Ionic_interactions_and_p_H_influence_on_Anion_Exchange_Chromatography_AEX_612e231ba4.jpg" alt="KNAUER: Oligos Made Easy - Part 2 AEX: Figure 3 Ionic interactions and pH influence on Anion Exchange Chromatography (AEX)." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_KNAUER_Oligos_Made_Easy_Part_2_AEX_Figure_3_Ionic_interactions_and_p_H_influence_on_Anion_Exchange_Chromatography_AEX_612e231ba4.jpg 245w," sizes="100vw" width="1547" height="593"><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/KNAUER_Oligos_Made_Easy_Part_2_AEX_Figure_4_AEX_buffer_selection_and_gradient_strategy_chromatogram_with_red_buffer_gradient_curve_53fd1bb11b.jpg" alt="KNAUER: Oligos Made Easy - Part 2 AEX: Figure 4 AEX buffer selection and gradient strategy (chromatogram with red buffer gradient curve)." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_KNAUER_Oligos_Made_Easy_Part_2_AEX_Figure_4_AEX_buffer_selection_and_gradient_strategy_chromatogram_with_red_buffer_gradient_curve_53fd1bb11b.jpg 245w," sizes="100vw" width="1600" height="327"><h4>AEX as the Gold Standard for Oligonucleotide Separation</h4>Anion exchange chromatography has become the method of choice for both analytical and preparative oligonucleotide workflows. Its advantages include:<ul><li>High resolving power: Closely related impurities, including n–1 or n–2 species, can often be separated and quantified.</li><li>Predictable retention behavior: In many cases, retention time correlates linearly with oligonucleotide length, simplifying identification.</li><li>Compatibility with modified oligonucleotides: Even chemically modified sequences retain their anionic nature, allowing AEX to resolve both products and related impurities.</li><li>Excellent control via gradient elution: Salt gradients provide precise tuning of selectivity and resolution.</li></ul>Typically, shorter or degraded oligonucleotides elute at lower ionic strength, while full-length products with higher charge density appear later in the chromatogram.<img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/KNAUER_Oligos_Made_Easy_Part_2_AEX_Figure_5_AEX_Advantages_and_Challenges_7835521e34.jpg" alt="KNAUER: Oligos Made Easy - Part 2 AEX: Figure 5 AEX Advantages and Challenges." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_KNAUER_Oligos_Made_Easy_Part_2_AEX_Figure_5_AEX_Advantages_and_Challenges_7835521e34.jpg 245w," sizes="100vw" width="1535" height="707"><h4>Final Perspective: Mastering AEX with Confidence</h4>Anion exchange chromatography may appear complex at first, but with thoughtful optimization of pH, buffer composition, and gradient conditions, it becomes a highly precise and reliable tool for oligonucleotide analysis. When properly applied, AEX delivers sharp peaks, excellent resolution, and robust performance—even for challenging sequences and modifications.For laboratories developing or refining oligonucleotide workflows, AEX offers both analytical depth and operational confidence.<blockquote>If you would like to discuss AEX method development or optimization for oligonucleotides, feel free to contact us at sales@knauer.net. More insights into practical oligonucleotide analysis will follow in our “Oligos Made Easy” series.For technical questions or in-depth discussion, the author can be reached at kindler@knauer.net</blockquote>

Oligos Made Easy - Part 2: AEX

News from LabRulezLCMS Library - Week 07, 2026

Article

Application

Plastics

Environment

Science and research

Poster

RECETOX (Research Centre for Toxic Compounds in the Environment) is an independent department of the Faculty of Science of Masaryk University in Brno, which has been established in 1983. RECETOX is focused on the research and education in cross-cutting area of Environment and Health. It addresses interactions among chemicals, environment, and biological systems, including consequences at local, regional, and global levels.

RECETOX - Centrum pro výzkum toxických látek v prostředí

Everything from the world of analytical chemistry in one place. We connect people in solving their problems. At Labrulez you will find all the necessary information easily, quickly and clearly. Stop searching and start finding.

LabRulez

Based in Berlin, KNAUER is a medium-sized, owner-managed company that has been serving the sciences since 1962. We develop and manufacture scientific instruments of superior quality for liquid chromatography, including: Analytical HPLC/UHPLC, Preparative HPLC, Fast protein liquid chromatography (FPLC), Multi-column chromatography/Simulated moving bed (SMB) chromatography. High pressure dosing, in-line detection systems, and osmometry are additional business areas.

KNAUER

Agilent Technologies

Phenomenex

Thermo Fisher Scientific

Shimadzu Corporation

Dr. Maisch High Performance LC GmbH

Waters Corporation

When we encounter unexpected things with our chromatography, we wonder why did that happen? As we look at these problems, we’ll also take a closer look at our sample and mobile phase to better understand how they may be contributing to our chromatography problems.
A better understanding of where problems can come from helps us narrow down the possibilities, solve our problem faster, and identify ways to prevent them from happening in the future.
<blockquote>
Presenter: Golnar Javadi (Application Engineer, Agilent Technologies, Inc.)
</blockquote>
<blockquote>
Golnar Javadi has a Master’s degree in Chemical Engineering with more than 20 years of experience in the field of analytical chemistry. Prior to her current role, she taught Chemistry at college level for 5 years and worked in the pharmaceutical and nutraceutical industries where she obtained firsthand experience with analytical method development and sample preparation techniques.
</blockquote>
<blockquote>
Golnar then joined Varian as a technical support specialist, providing customers with method development assistance and technical support on Sample Preparation (SPE, SLE, and Filtration), HPLC, GC, ToxiLab and DAT. After the Varian-Agilent merger, Golnar joined Agilent Technologies as an Applications Engineer. She provides technical support to customers on Sample Preparation products, Liquid Chromatography columns, and Spectroscopy supplies.
</blockquote>

WHY Did That Happen?

This webinar will look at available resources to help meet your chromatography needs like:
<ul>
<li>Who can help me with method development questions, like what column to choose?</li>
<li>How can I get help with troubleshooting?</li>
<li>Where can I find videos to help with my instrument maintenance?</li>
<li>Is there a forum where I can ask other chromatographers for their input?</li>
</ul>
We’ll talk about these and other resources to help you be successful.
<blockquote>
Presenter: Golnar Javadi (Application Engineer, Agilent Technologies, Inc.)
</blockquote>
<blockquote>
Golnar Javadi has a Master’s degree in Chemical Engineering with more than 20 years of experience in the field of analytical chemistry. Prior to her current role, she taught Chemistry at college level for 5 years and worked in the pharmaceutical and nutraceutical industries where she obtained firsthand experience with analytical method development and sample preparation techniques.
</blockquote>
<blockquote>
Golnar then joined Varian as a technical support specialist, providing customers with method development assistance and technical support on Sample Preparation (SPE, SLE, and Filtration), HPLC, GC, ToxiLab and DAT. After the Varian-Agilent merger, Golnar joined Agilent Technologies as an Applications Engineer. She provides technical support to customers on Sample Preparation products, Liquid Chromatography columns, and Spectroscopy supplies.
</blockquote>
<blockquote>
Presenter: Rita Steed (Application Engineer, Agilent Technologies, Inc.)
</blockquote>
<blockquote>
Rita Steed began supporting the LC column line for Agilent in 1999. As a Chromatography Specialist (first with Chromatography, Inc., then directly for Agilent), Rita worked on-site with researchers in Pharmaceutical and other industries presenting technical seminars and assisting researchers with troubleshooting and method development. Rita has over 20 years of Chromatography experience in the Biotechnology, Chemical, and Pharmaceutical industries. She has held positions in Research, Sales, and Technical Service. Rita has earned degrees in Microbiology and Life Sciences/Biochemistry. In her current position, she is an inside Application Engineer supporting LC columns.
</blockquote>
<blockquote>
Presenter: Mark Powell (Application Engineer, Agilent Technologies, Inc.)
</blockquote>
<blockquote>
Mark Powell is an Applications Engineer located at Agilent’s Little Falls site in Wilmington, DE. Before joining Agilent in 2011, Mark worked in the pharmaceutical industry synthesizing, purifying and analyzing drug candidates. He provides applications assistance and technical support for Agilent’s HPLC columns and consumables.
</blockquote>
<blockquote>
Presenter: Jean Lane (Application Engineer, Agilent Technologies, Inc.)
</blockquote>
<blockquote>
Jean has been employed with Agilent since Nov 2010 in technical and applications support and has a primary role supporting Agilent’s full line of LC columns and consumables, which also includes support for Agilent’s GPC/SEC product line. She came to Agilent thru acquisition of Varian and the former Polymer Laboratories. Jean has 20 plus years of technical and applications support for both HPLC and GPC consumable products. In addition to practical chromatography experience in the laboratory, she has had many, many years in her technical role involving customer support providing assistance and troubleshooting for customer chromatography issues.
</blockquote>
<blockquote>
Presenter: Paul Altiero (Application Engineer, Agilent Technologies, Inc.)
</blockquote>
<blockquote>
Paul Altiero is an Applications Engineer located at the Agilent Little Falls site in Wilmington, DE. Prior to coming to Agilent in 2009, Paul worked in the pharmaceutical industry performing LCMS bioanalysis supporting Drug Discovery DMPK. While at Agilent, Paul has been a field service engineer for LCMS as well as the integrating program manager for the RapidFire high throughput LCMS system. In his current role Paul’s responsibilities include technical support and application assistance for LCMS consumables.
</blockquote>

Agilent Technologies: WHO Do I Turn To? Chromatography Resources

WHO Do I Turn To? Chromatography Resources

Cultivation-independent sequencing techniques have revealed a tremendous diversity of microorganisms on our planet. Although, the majority of microorganisms are not isolated and do not have sequenced genomes. While this applies to all fields of environmental microbiology, it does not prevent research from progressing. Conversely, in the field of subsurface microbiology the dearth of cultured representatives heavily restricts the simplest hypothesis driven experimentation.
In this work, we discovered a novel strict anaerobic thermophilic bacterium from a monitoring borehole at a depth of 691 meters below land surface. This borehole is of particular interests as it resides within the US Great Basin, in particular the Nevada National Security Site, underlain by the Death Valley Regional Flow System. The characterized isolate was given the name Thermoanaerosceptrum fracticalcis strain DRI-13T, and the sequenced genome coupled with methylome analysis reveal interesting insights into metabolism/energy conservation, gene regulation, DNA defences, and archival of previous viral aggressions. This microorganism and others have been analysed using HPLC to track substrate utilization and waste product production in order to better understand the syntrophic interactions of subsurface life. The deep biosphere may reveal itself as an untapped resource of microbial biodiversity holding novel solutions to industrial, medical, and potential providing answer to the origins of life.
Our panel will host a Q&amp;A session after the presentation to address any questions or comments. 
<blockquote>
Presenter: Scott D. Hamilton-Brehm, Ph.D. (Assistant Professor Department of Microbiology, Southern Illinois University)
</blockquote>
<blockquote>
Scott Hamilton-Brehm received his B.S. (1997) from San Louis Obispo in San Louise Obispo, CA. where he studied bacteria inside the abdomens of prehistoric bees trapped in amber. He worked for the biotech company Promega Biosciences for 4 years before returning to academia. He received his Ph.D. in biochemistry and molecular biology (2008) from University of Georgia (UGA) in Athens, GA. At UGA he studied the model hyperthermophilic archaeon Pyrococcus furiosus and how it reacted to iron-sulfur-nitrogen based antibiotic molecules. He discovered his talent and skill of culturing thermophilic microorganisms. This skill set led him to work as a Postdoctoral Research Associate (2008-2012) at Oak Ridge National Laboratory (ORNL). At ORNL he work for the BioEnergy Science Center (BESC) whose mandate was to research the application microbial mediated consolidated bio-processing (CBP) to generate biofuels. Afterwards, Scott was part of the Division of Earth and Ecosystem Sciences (DEES) at the Desert Research Institute in Las Vegas, NV (2012-2016), where he studied and cultivated strict anaerobic thermophilic microbial communities from the deep subsurface of the Southern Nevada Desert. Now he is part of the Microbiology Department at Southern Illinois University of Carbondale (SIUC), where he is continuing his research into culturing deep subsurface microbes and bacterial driven biofuel production. During the beginning of the 2020 COVID-19 pandemic, Chicago was fighting against becoming a new hot spot for the virus, testing in Illinois slowed when the availability of viral transport medium (VTM) became strained. The governor of Illinois made an emergency call to university researchers to make VTM in an effort protect the state’s citizens. Hamilton-Brehm’s laboratory rallied, pulling support from the departments of microbiology and zoology at SIUC to answer the governor’s call. Professors and student volunteers together produced 115K vials of VTM for the state of Illinois over two months.
</blockquote>
<blockquote>
Presenter: Mathew Chaidez B.S. (Field Sales Engineer, Shimadzu)
</blockquote>
<blockquote>
<ul>
<li>4 years in the 82nd Airborne Division from 2000-04</li>
</ul>
</blockquote>
<ul>
<li>B.S. in Chemistry from Saint Louis University</li>
<li>5 years analytical experience in a radiopharmaceutical lab</li>
<li>5 years sales with Shimadzu</li>
</ul>

Shimadzu: Subsurface Microbiology: Metabolism by HPLC

Subsurface Microbiology: Metabolism by HPLC

Agilent’s MassHunter WalkUp simplifies routine assays that allows all users access to instrumentation for their samples. WalkUp is a software interface with predefined users and acquisition methods for both LC and LC/MS instrumentation.
Here we will provide an introduction to WalkUp briefly discussing the user experience from the administrator (setting up groups, users, methods, data analysis confirmation) and users’ (running samples) perspectives. We will highlight a few workflows including small molecule mass analysis to large intact protein confirmation.
<blockquote>
Presenter: Caroline Chu (LC/MS Application Scientist, Agilent Technologies, Inc.)
</blockquote>
<blockquote>
Caroline is an LC/MS Application Scientist with Agilent with over 7 years experience supporting the LC-Q/TOF, QQQ, and IM-QTOF MS and automation, including AssayMap and Rapid Fire. Her focus is on BioPharma applications including intact proteins, native MS, glycomics, high-throughput screening and life science applications including proteomics, targeted proteomics and metabolomics.
</blockquote>
<blockquote>
Prior to joining Agilent she completed her undergraduate work at San Francisco State University in Biochemistry before moving on to her Ph.D in Biophysics at the University of California, Davis, working on clinical glycomics with high resolution MS, under the guidance of Professor Carlito Lebrilla. She completed her post-doctoral work at the Institute for Systems Biology in Seattle, WA working targeted proteomics on the human proteome under the guidance of Robert Moritz and in collaboration with Lee Hood and Ruedi Abersold.
</blockquote>

Agilent Technologies: Mass Spectrometry for the Masses, Open Access LC and LC/MS  Using Agilent WalkUp

Mass Spectrometry for the Masses, Open Access LC and LC/MS  Using Agilent WalkUp

Authorities expects from all manufacturers to prevent or reduce the presence of nitrosamines in active pharmaceutical ingredients (APIs) and drug products.
On March 31st, 2021, the EMA guidance for Marketing Authorization Holders will engage a new milestone, called Step 2, and related to the process of products identified to be at risk of N-nitrosamine formation. 
To give you all decision factors on the confirmatory testing, Eurofins BioPharma Product Testing and Waters are glad to invite you to this joined webinar, going through the latest regulatory requirements for testing nitrosamines in solvents, drug substance and drug products.
<ul>
<li>Eurofins’ scientists will provide examples of screening methods performed in their facility located in Toulouse, France.
</li>
<li>Waters will explain how critical it is to combine both LC and MS aspects to quantify traces in highly concentrated matrices.
</li>
</ul>
<blockquote>
Presenter: Elodie Barrau (Site Manager, Eurofins Amatsi Analytics France)
</blockquote>
<blockquote>
Elodie Barrau is the Site Manager of Eurofins Amatsi Analytics in Toulouse area, France, with focus in chemistry, manufacturing and control, scientific support (GLP and GMP) for international preclinical, pharma, clinical development and/or registration, and implementation of analytical analysis. Elodie has a degree in Agronomist Engineering specialising in Phytochemistry Drug Development.
</blockquote>
<blockquote>
Presenter: Emmanuel Desmartin (Mass Spec and Bioanalysis Manager, Eurofins Amatsi Analytics France)
</blockquote>
<blockquote>
Emmanuel Desmartin is Mass Spec and Bioanalysis Lab Manager at Eurofins Amatsi Analytics, with 15 years of experience in bioanalysis; holding different positions in CROs as study director in development and validation of LC-MS methods, management of mass spec lab, and technical support.
</blockquote>
<blockquote>
Presenter: Arjan Timmerman (Pharmaceutical Marketing Manager EMEA Pharma, Compliance and Impurities, Waters European Head Quarters)
</blockquote>
<blockquote>
Arjan Timmerman, Experienced Pharmaceutical Marketing Manager with a demonstrated history of working in Regulated Pharmaceutical Industry, is skilled in Life Sciences, Impurities, Compliance and Data Integrity, Method Development and Validation.
</blockquote>

Waters: Nitrosamines Analysis (Step 2): Confirmatory testing

Nitrosamines Analysis (Step 2): Confirmatory testing

Enantiomers are compounds which are non-superimposable mirror image of each other and are challenging to separate without a streamlined method development strategy. Separation, identification and quantification of Chiral isomer ensures safety and efficacy. In addition, food flavors, new pesticide classes are also looked at for enantiomeric separation.
A simple method development strategy will be discussed to optimize chiral separation. Further tips and tricks on solvent switching, Normal phase, reverse phase, polar organic and SFC modes will be discussed on immobilized and coated chiral phases. In addition, scalability aspects from analytical to preparative chromatography will also be explored.
<blockquote>
Presenter: Ramkumar Dhandapani, PhD (Global Product Manager, Phenomenex)
</blockquote>

Phenomenex: Strategies, Tips and Tricks for Simplified Chiral Method Development

Strategies, Tips and Tricks for Simplified Chiral Method Development

<h3>Basics and beyond</h3>
A wide range of detection options exist for liquid chromatography, but each has limitations. Charged aerosol detection (CAD) offers an alternative, universal detection option.
<h3>Presentations:</h3>
<h5>Basics and beyond</h5>
<blockquote>
Paul Gamache and Chris Tuczemskyi - Thermo Fisher Scientific
</blockquote>
<h5>Polysorbate analysis in drug products</h5>
<blockquote>
Georg Schuster - Coriolis Pharma
</blockquote>
<h5>CAD in Pharmaceutical Analysis – Selected applications and optimization strategies</h5>
<blockquote>
Ruben Pawellek - Institut für Pharmazie und Lebensmittelchemie University of Wuerzburg 
</blockquote>
<h5>High Throughput Analysis (HTA) for small molecule libraries with the Thermo Scientific Vanquish Duo UHPLC system</h5>
<blockquote>
Thomas Dann and Matthew Gill - Charles River Laboratories
</blockquote>
<h5>Roundtable and Q and A discussion</h5>
<h3>Learning points:</h3>
<ul>
<li>Learn how charged aerosol detection works </li>
<li>What makes charged aerosol detection so powerful </li>
<li>What benefits it offers </li>
<li>What applications are ideal for charged aerosol detection?</li>
</ul>
<h3>Who should attend:</h3>
<ul>
<li>Laboratory managers and Directors</li>
<li>Technology leaders </li>
<li>Principal scientists </li>
<li>Laboratory analyst</li>
</ul>
<blockquote>
Presenter: Chris Tuczemskyi (Applications &amp; Technical Support Specialist, Thermo Fisher Scientific)
</blockquote>
<blockquote>
Chris Tuczemskyi is a chromatography specialist at Thermo Fisher Scientific, based in Cambridge, UK. After spending a number of years building up his experience with chromatography instruments in a water testing laboratory, he joined Thermo Fisher Scientific in 2014 as a chromatography service engineer for Unity Lab Services. Here he developed his passion for supporting customers using HPLC and in 2018 moved into the application specialist team. As a chromatography specialist, his role includes working with customers in developing applications to help to enable them to make the world healthier, cleaner and safer. In 2009, Chris graduated from the University of Essex in Biochemistry (Bsc Hons).
</blockquote>
<blockquote>
Presenter: Georg Schuster (Coriolis Pharma)
</blockquote>
<blockquote>
Georg Schuster is senior scientist and group leader at Coriolis Pharma, a science-driven contract research organization, where he focusses on protein characterization and analytic method development for biopharmaceuticals with LC and CE based separation techniques and polysorbate characterization via LC coupled to charged aerosol detection. Georg has received his PhD degree in analytical chemistry from the University of Vienna, Austria, in the field of HILIC stationary phase development and further worked as post-doctoral scientist at the Australian Centre for Research on Separation Science, Tasmania. Prior to working at Coriolis Pharma (2016), he was a scientist engaged in protein separation method development at Novo Nordisk, Denmark.
</blockquote>
<blockquote>
Presenter: Ruben Pawellek (PhD Student, University of Wuerzburg, Germany)
</blockquote>
<blockquote>
Ruben Pawellek received his pharmacy degree at the university of Wuerzburg in 2016, followed by one year of practical training in the quality control unit of Boehringer Ingelheim and in a public pharmacy. Since 2017, he pursues his PhD studies in pharmaceutical chemistry at the university of Wuerzburg. His research is mainly focused on the application of HPLC-CAD in pharmaceutical analysis and on the optimization of the detector’s performance. In 2020, he completed a three-year professional training in pharmaceutical analysis.
</blockquote>
<blockquote>
Presenter: Thomas Dann (Analytical Scientist, Charles River Labs)
</blockquote>
<blockquote>
Graduating from the University of East Anglia with an MChem degree, I went on to complete a PhD there focusing on electroanalytical chemistry, where I synthesised organometallic compounds, characterising them by NMR, mass spectrometry and electrochemical techniques. From this I moved into industry working in a Compound Management lab where the role included QC by LCMS for newly solubilised compounds. Building on this experience, I then progressed onto a full analytical science role at Charles River.
</blockquote>
<blockquote>
Presenter: Matthew Gill (Charles River Labs)
</blockquote>
<blockquote>
Moderator: Frank Steiner (Senior Manager of Product Applications, Thermo Fisher Scientific)
</blockquote>
<blockquote>
Frank Steiner is Senior Manager of Product Applications and coordinates HPLC based scientific collaborations in Thermo Fisher Scientific as scientific advisor. He joined Dionex Corporation in 2005, now a part of Thermo Fisher Scientific, and had been manager in various HPLC marketing functions. Frank received his PhD degree in chemistry in 1995 from Saarland University in Saarbruecken, Germany where he became assistant professor in 1997 and associate professor in 2003, after his postdoc at the nuclear research center in Saclay, France in 1996. Frank published more than 30 publications in refereed journals and more than 10 text book chapters on HPLC topics.
</blockquote>
<blockquote>
Moderator: Paul Gamache (Director Research Development, Thermo Fisher Scientific)
</blockquote>
<blockquote>
Paul is an analytical chemist who joined Thermo Fisher Scientific in 2011 through acquisition of Dionex Corporation. He is a Director of R &amp;D responsible for development of analytical technologies with primary focus on charged aerosol detection (CAD) and electrochemical detection (ECD) for LC. Prior to joining Dionex in 2009, Paul led the development of first and second generation Corona CAD products. He has published more than 50 articles and book chapters and was co-awardee of an NIH Roadmap grant for development of hyphenated EC and LC-MS technologies for metabolomics research. Paul is editor and contributing author to the book “Charged Aerosol Detection for Liquid Chromatography and Related Separation Techniques” published in 2017 by John Wiley &amp; Sons, Inc.
</blockquote>

Thermo Scientific: Charged Aerosol Detection Symposium

Charged Aerosol Detection Symposium - Session 1

<h3>Pharma and biopharma applications</h3>
With the flexibility and performance for analytical R&amp;D, and the simplicity and reproducibility needed for manufacturing QA/QC, charged aerosol detection can be used for the analysis of pharmaceuticals (large and small molecule). Learn how CAD can be applied in your work with tips, tricks, pointers, and expert advice from our guest speakers.
<h3>Presentations:</h3>
<h5>Peptide Quantification by CAD</h5>
<blockquote>
Kilian Conde Frieboes - Novo Nordisk 
</blockquote>
<h5>How CAD enabled universal quantification in a high throughput library purification platform</h5>
<blockquote>
Marcel Reck - Novartis Institute for Biomedical Research
</blockquote>
<h5>LC-Charged aerosol detector in early and late-stage pharmaceutical development: Selection of regression models at optimum power function value</h5>
<blockquote>
Imad Haidar Ahmad - Merck Research Laboratories 
</blockquote>
<h5>Roundtable and Q and A discussion</h5>
<blockquote>
With Special guest Ulrike Holzgrabe, Institut für Pharmazie und Lebensmittelchemie University of Wuerzburg 
</blockquote>
<h3>Learning points:</h3>
<ul>
<li>Understand the technology you are using and set it up correctly</li>
<li>CAD is reliable and an easy solution to quantify novel compounds without the need for compound specific calibration </li>
<li>CAD is a key technology in our high throughput library purification platform </li>
<li>Using quantitative LC-CAD in highly regulated laboratories e.g., GLP, GMP</li>
</ul>
<h3>Who should attend:</h3>
<ul>
<li>Laboratory managers and directors </li>
<li>Technology leaders </li>
<li>Principal scientists </li>
<li>Laboratory analyst</li>
</ul>
<blockquote>
Presenter: Kilian Conde-Frieboes (Principal Scientist, Novo Nordisk)
</blockquote>
<blockquote>
Studied Chemistry in Kiel/Germany.Doctor of Science in 1992 (Asymmetric Synthesis)Post docs at the University of Frankfurt and University of California, San Diego (1992-1995).Assistant at the ETH Zurich (1995-1997).Joined Novo Nordisk in 1997.
</blockquote>
<blockquote>
Presenter: Marcel Reck (Research Scientist II, Novartis Institutes for BioMedical Research (NIBR), Switzerland)
</blockquote>
<blockquote>
Before I joined Novartis in 2006, I have previously finished my apprenticeship at the Johannes Gutenberg University in Mainz in 2004 and worked at the Julius Maximilians University of Würzburg. I started my research career at Novartis in Basel as a Scientific Associate supporting Global Discovery Chemistry research projects in the area of Autoimmunity/Transplantation and Inflammation. In 2015, I took an opportunity to focus on analytical and purification research optimizing small molecule separations in the area of analytical and preparative Supercritical Fluid Chromatography and RP HPLC. My organic chemistry expertise has enabled me the opportunity to collaborate more broadly with medicinal chemistry teams and this has recently led to me speer-heading a plate based library initiative focussing on best practice and efficient automated QC, purification and quantification using the CAD detector for MilliLibraries (2-5mg scale) of MedChem research projects.
</blockquote>
<blockquote>
Presenter: Imad Haidar Ahmad, Ph.D (Associate Principal Scientist and supervisor, Merck Rahway, USA)
</blockquote>
<blockquote>
Imad A. Haidar Ahmad received his Ph.D. from Florida State University under the mentorship of Dr. André Striegel. He completed his postdoctoral research with Prof. Peter Carr at University of Minnesota. He is currently an Associate Principal Scientist and supervisor in the Analytical Chemistry Enabling Technology group within MRL’s AR&amp;D department.
</blockquote>
<blockquote>
Moderator: Frank Steiner (Senior Manager of Product Applications, Thermo Fisher Scientific)
</blockquote>
<blockquote>
Frank Steiner is Senior Manager of Product Applications and coordinates HPLC based scientific collaborations in Thermo Fisher Scientific as scientific advisor. He joined Dionex Corporation in 2005, now a part of Thermo Fisher Scientific, and had been manager in various HPLC marketing functions. Frank received his PhD degree in chemistry in 1995 from Saarland University in Saarbruecken, Germany where he became assistant professor in 1997 and associate professor in 2003, after his postdoc at the nuclear research center in Saclay, France in 1996. Frank published more than 30 publications in refereed journals and more than 10 text book chapters on HPLC topics.
</blockquote>
<blockquote>
Moderator: Paul Garnache (Director Research Development, Thermo Fisher Scientific)
</blockquote>
<blockquote>
Paul is an analytical chemist who joined Thermo Fisher Scientific in 2011 through acquisition of Dionex Corporation. He is a Director of R &amp;D responsible for development of analytical technologies with primary focus on charged aerosol detection (CAD) and electrochemical detection (ECD) for LC. Prior to joining Dionex in 2009, Paul led the development of first and second generation Corona CAD products. He has published more than 50 articles and book chapters and was co-awardee of an NIH Roadmap grant for development of hyphenated EC and LC-MS technologies for metabolomics research. Paul is editor and contributing author to the book “Charged Aerosol Detection for Liquid Chromatography and Related Separation Techniques” published in 2017 by John Wiley &amp; Sons, Inc.
</blockquote>
<blockquote>
Presenter: Prof. Dr. Ulrike Holzgrabe (University of Würzburg)
</blockquote>
<blockquote>
Prof. Dr. Ulrike Holzgrabereceived her diploma degree in Chemistry at the University of Marburg in 1979, her approbation in Pharmacy in 1982, and her PhD in Pharmaceutical Chemistry in 1983 both at the University of Kiel. After habilitation in Pharmaceutical Chemistry in Kiel 1989 she became an associate professor at the University of Bonn (1990-1999). In 1988, she was a post-doc fellow at the University of Bath, UK, with A. Casy, in 1994 a visiting professor at the University of Erlangen with J. Gasteiger, and 1995 at the University of Illinois at Chicago with A.F. Hopfinger. Since 1999 she holds a full professorship at the University of Würzburg (declining offers from the universities of Tübingen, Münster and Berlin, and the CEO position of the BfArM). She served as vice-rector of the University of Bonn from 1997-1999 and as a vice-president of the University Wuerzburg from 2018-2021. She was president of the German Pharmaceutical Society from 2004 till 2007. She is/was a member of the German and European Committee of the Pharmacopoeia (BfArM, EDQM), and a member of the scientific boards of the BfArM, BPS of FIP, and the HIPS as well as a collegiate of the DFG. In 2020, she received the “Bayerischen Verdienstorden” (Bavarian order of merit). Her research interests are the development of antiinfectives and GPCR ligands as well as quality analysis of drugs.
</blockquote>
<blockquote>
She is co-owner of Aamuthera, a company dealing with the development of anticancer drugs.
</blockquote>

Charged Aerosol Detection Symposium - Session 2

<h3>Food and beverage applications</h3>
Did you know that CAD can be used in application areas such as food and beverages, in lipid analysis for food fraud and for sugar fingerprinting? It’s also used to detect specialty chemicals and polymers, such the environmentally toxic agent polyacrylic acid.
<h3>Presentations:</h3>
<h5>CAD Tips and Tricks</h5>
<blockquote>
Paul Gamache and Chris Tuczemskyi - Thermo Fisher Scientific
</blockquote>
<h5>A UHPLC-PDA-CAD-HRMS Platform for Comprehensive Identification and Quantitation of Constituents in Complex Mixtures for Safety Risk Assessments</h5>
<blockquote>
Dr. Jason Price - Procter &amp; Gamble R&amp;D 
</blockquote>
<h5>Using UHPLC-CAD and PCA to detect olive oil adulteration via triacylglycerol analysis</h5>
<blockquote>
Hilary Green - UC Davies
</blockquote>
<h5>Roundtable and Q and A discussion</h5>
<h3>Learning points:</h3>
<ul>
<li>How food adulteration can be quickly detected</li>
<li>Predict the level of adulteration and the adulterant</li>
<li>Quantitation for analytes where analytical reference standards are not available</li>
<li>Using CAD with other detectors</li>
</ul>
<h3>Who should attend:</h3>
<ul>
<li>Laboratory managers and directors </li>
<li>Technology leaders </li>
<li>Principal scientists </li>
<li>Laboratory analyst</li>
</ul>
<blockquote>
Presenter: Jason M. Price (Principal Scientist, Procter &amp; Gamble, USA)
</blockquote>
<blockquote>
Jason Price received his Ph.D. in Chemistry from Purdue University in 2002 where he worked with Professor Hilkka Kenttämaa to understand the factors that influence the fundamental reactivity of organic radicals and biradicals via their ion-molecule reactions in Fourier transform ion cyclotron resonance mass spectrometers. After graduation, Jason joined Procter &amp; Gamble in Cincinnati, OH working in Beauty Analytical to drive upstream technologies to in-market products across Personal Cleansing, Skin Care and Hair Care categories. He developed expertise in quantifying deposition and bioavailability of actives to guide formulation development and claim support. Jason is currently a Principal Scientist in P&amp;G’s Trace Analytical Capability where he has worked since 2009. In this role, he supports all P&amp;G businesses by leading (1) the development of quantitative LC-MS/MS assays and (2) the identification of unknowns (e.g., impurities, degradation products, contaminants) in complex mixtures by high-resolution mass spectrometry.
</blockquote>
<blockquote>
Presenter: Hilary Green (Graduate student, Ph.D. Candidate, University of California, Davis USA)
</blockquote>
<blockquote>
Hilary’s is a Ph.D. candidate in the Agricultural and Environmental Chemistry group at the University of California, Davis. Her interest in research interests stem from her passions for food and the environment through the lens of analytical chemistry. Her dissertation focuses on ensuring the authenticity and quality of edible oils. She has published a faster and less wasteful method to detect olive oil adulteration. She also published a work evaluating the chemical composition of avocado oils on the US market. For the final part of her dissertation, she is working to understand the natural variance in avocado oils to support the standard development effort for this oil. In addition, she is developing a better method to detect adulteration in avocado oil. Hilary’s research is closely connected to the food industry and consumers; thus, she is broadly interested in educating the public on scientific research.
</blockquote>
<blockquote>
Presenter: Ulrike Holzgrabe (Univ of Wuerzburg)
</blockquote>
<blockquote>
Moderator: Frank Steiner (Senior Manager of Product Applications, Thermo Fisher Scientific)
</blockquote>
<blockquote>
Frank Steiner is Senior Manager of Product Applications and coordinates HPLC based scientific collaborations in Thermo Fisher Scientific as scientific advisor. He joined Dionex Corporation in 2005, now a part of Thermo Fisher Scientific, and had been manager in various HPLC marketing functions. Frank received his PhD degree in chemistry in 1995 from Saarland University in Saarbruecken, Germany where he became assistant professor in 1997 and associate professor in 2003, after his postdoc at the nuclear research center in Saclay, France in 1996. Frank published more than 30 publications in refereed journals and more than 10 text book chapters on HPLC topics.
</blockquote>
<blockquote>
Moderator: Paul Gamache (Director Research Development, Thermo Fisher Scientific)
</blockquote>
<blockquote>
Paul is an analytical chemist who joined Thermo Fisher Scientific in 2011 through acquisition of Dionex Corporation. He is a Director of R &amp;D responsible for development of analytical technologies with primary focus on charged aerosol detection (CAD) and electrochemical detection (ECD) for LC. Prior to joining Dionex in 2009, Paul led the development of first and second generation Corona CAD products. He has published more than 50 articles and book chapters and was co-awardee of an NIH Roadmap grant for development of hyphenated EC and LC-MS technologies for metabolomics research. Paul is editor and contributing author to the book “Charged Aerosol Detection for Liquid Chromatography and Related Separation Techniques” published in 2017 by John Wiley &amp; Sons, Inc.
</blockquote>

Charged Aerosol Detection Symposium - Session 3

We invite you to join our free webinar to understand the principles of chiral HPLC, different types of stationary phases and their characteristics and separation modes and to learn more about Dr. Maisch HPLC&#39;s solutions to your separation challenges.
<blockquote>
Presenter: Guido Krautz (Business Development Manager bei Dr. Maisch High Performance LC GmbH)
</blockquote>

LCMS webinars focusing on HPLC - page 61

Chiral HPLC: From Basics to the Latest and Unique Novelties