Learn how to recognize and search in-source fragmentation spectra in NIST26 using chromatogram results, Score (Unfiltered), and MS/MS Library Search for compound identification.

A practical guide to processing LC-MS/MS data in the NIST26 Chromatogram Window, from parameter setup and library searching to identification review and result export.

Discover the new NIST26 LC-MS/MS workflow with integrated chromatogram analysis, expanded MS/MS libraries, hybrid searching, and advanced tools for confident unknown compound identification.

Learn how to configure, customize, and navigate the new NIST 26 Chromatogram Window. This practical guide covers layouts, zooming, filtering, structure display, and efficient data review workflows.

This course shows how to identify unknowns in LCMS analysis employing the NIST MSMS search software. Part 5 discusses the the determination of molecular formulae with Thermo Freestyle software.

This course shows how to identify unknowns in LCMS analysis employing the NIST MSMS search software. Part 3 discusses basic settings for Thermo Freestyle software and the NIST 2023 Search.

This course shows how to identify unknowns in LCMS analysis employing the NIST MSMS search software. Part 2 discusses the alternate methods for processing the data.

Part 1A discusses the using the "Nearby Precursor" to easily display a MSMS spectrum associated with an ion in MS.

This course shows how to identify unknowns in LCMS analysis by the NIST MSMS search software. Part 4 demonstrates 3 different type searches for MSMS data within the NIST2023 search software.

This part discusses my favorite approach for processing Orbitrap LCMS/LCMSMS data for identificaiton of unknowns using the NIST2023 search and associated libraries.