KNAUER AZURA® Lab Prep HPLC System

AZURA® Preparative HPLC Customized purification

AZURA® preparative systems are the perfect solution for frequently changing separation tasks – from milligram to kilogram scale. Design your AZURA preparative system to your needs and combine flexibility and reliability.

Customaze your system

AZURA® Prep systems are tailor-made for you. Configure your system from injection to detection and choose between different materials, flow rates, valves, and detectors.

Easy scale up from compact to pilot systems

Due to the flexible design of our devices, you can easily change parts like pump heads or flow cells and integrate all components of the compact into the pilot-scale system.

Stacked injections, peak & solvent recycling

AZURA® Prep systems can be used for special separation modes like peak recycling and stacked injections. We help you to configure your system and choose the best software for you.

Flexibility and performance

KNAUER AZURA® Prep HPLC System.

AZURA® Lab Prep HPLC System

The Lab Prep LC system is designed for your more demanding semi-preparative separations. You can customize a highly flexible LC system with the freely combinable components. With a maximum flow rate of 50 ml/min, it is possible to separate up to several hundred milligrams per run.

KNAUER: AZURA® Lab Prep HPLC System .

• Lab Prep HPLC system with binary high-pressure gradient
• Up to 200 bar operating pressure
• Injection volumes up to 10 ml via sample pump or manual injection
• Injection system: Injection valve with 500 μl sample loop
• Detection system: UV/VIS detector with one variable wavelength
• Supports UVD (VWD, MWD, DAD), RID, FLD, ELSD, MS
• Column selection: Compatible with 4–20 mm column ID
• Fraction collection: Automated fraction collector
• Software:
  • Intuitive PurityChrom® for direct control and visualization
  • Enhanced preparative LC software with functional visualization
  • GMP (21 CFR part 11) compliant software and certificates for wetted parts
• User-friendly operation: Safe and easy-to-use system control

Method transfer from analysis of chamazulen to preparative scale

Chamomile plants are known for their medical properties, having among others anti-inflammatory, analgesic, and sedative effects. These are due to the various phenolic compounds, one of them being matricine, which is converted during the distillation process to chamazulene. The characteristic blue color of chamomile essential oils, known as “chamomile blue,” is due to chamazulene. It has anti-inflammatory and antioxidant activity. The present application tested preparative HPLC to purify chamazulene from commercially available “chamomile blue” oil.

KNAUER: AZURA® Lab Prep HPLC System.

Results

Fractionation/Purification

The separation of chamazulene was optimized on an analytical scale, and the two-step gradient method was transferred to a preparative scale. Chamazulene purification was performed on a C18 250×20 mm column at 25 ml/min. Fractionation of chamazulene was conducted using the threshold function of PurityChrom software.

KNAUER: AZURA® Lab Prep HPLC System: Fig. 1: Chromatogram of preparative separation of chamazulene blue, collected fraction highlighted in red, 1 ml sample injection

The collected fraction was analyzed by analytical HPLC and revealed nearly 100% purity. Chromatogram overlay of the fraction, chamazulene standard, and the sample clearly showed the successful purification of chamazulene (Fig. 2).

KNAUER: AZURA® Lab Prep HPLC System: Fig. 2: Chromatogram overlay

Furthermore, the comparison of the fraction spectra and chamazulene spectra (Fig. 3) confirmed that the purified fraction was chamazulene.

KNAUER: AZURA® Lab Prep HPLC System: Fig. 3: Spectral view of fraction (-) and of chamazulen standard (-)